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TGFBI cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TGFBI
Entrez Gene ID 7045
Full Name transforming growth factor beta induced
Synonyms BIGH3,CDB1,CDG2,CDGG1,CSD,CSD1,CSD2,CSD3,EBMD,LCD1
General protein information
Preferred Names

transforming growth factor beta induced

Names

transforming growth factor-beta-induced protein ig-h3
RGD-CAP
RGD-containing collagen-associated protein
beta ig-h3
betaig-h3
kerato-epithelin
transforming growth factor beta-induced 68kDa
transforming growth factor, beta-induced, 68kD

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q31.1

Summary This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008].
Disorder MIM:

601692

Disorder Html: Corneal dystrophy, Groenouw type I, 121900 (3); Corneal dystrophy,

mRNA and Protein(s)

mRNA Protein Name
NM_000358.2 NP_000349.1 transforming growth factor-beta-induced protein ig-h3 precursor



Pan troglodytes (chimpanzee) TGFBI XP_001169327.1
Gallus gallus (chicken) TGFBI NP_990367.1
Danio rerio (zebrafish) tgfbi NP_878282.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004428 XP_313713.5
Drosophila melanogaster (fruit fly) mfas NP_788650.1
Macaca mulatta (rhesus monkey) TGFBI XP_001111447.1
Homo sapiens (human) TGFBI NP_000349.1
Bos taurus (cattle) TGFBI NP_001192331.1
Mus musculus (house mouse) Tgfbi NP_033395.1
Canis lupus familiaris (dog) TGFBI XP_538640.3
Rattus norvegicus (Norway rat) Tgfbi NP_446254.1
Xenopus tropicalis (tropical clawed frog) tgfbi NP_001072329.1


Related articles in PubMed

Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis.
Kontostathi G, Zoidakis J, Makridakis M, Lygirou V, Mermelekas G, Papadopoulos T, Vougas K, Vlamis-Gardikas A, Drakakis P, Loutradis D, Vlahou A, Anagnou NP, Pappa KI
BioMed research international20174180703(2017)

Delayed Onset of Lattice Corneal Dystrophy Type IIIA Due to a Novel T621P Mutation in TGFBI.
Lee J, Ji YW, Park SY, Seo KY, Kim TI, Kim EK
Journal of refractive surgery (Thorofare, N.J. : 1995)32(5)356(2016 May)

TGF-β regulates TGFBIp expression in corneal fibroblasts via miR-21, miR-181a, and Smad signaling.
Choi SI, Jin JY, Maeng YS, Kim TI, Kim EK
Biochemical and biophysical research communications472(1)150-5(2016 Mar)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

LASIK surgery of granular corneal dystrophy type 2 patients leads to accumulation and differential proteolytic processing of transforming growth factor beta-induced protein (TGFBIp).
Poulsen ET, Nielsen NS, Jensen MM, Nielsen E, Hjortdal J, Kim EK, Enghild JJ
Proteomics16(3)539-43(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The p.(Val113Ile) mutation results in an alteration of the atypical LCD phenotype associated with the p.(Leu558Pro) mutation. This represents only the second report of the alteration of the phenotype of a TGFBI dystrophy by a second, non-homozygous pathogenic mutation, and thus provides insight into the phenotype-genotype correlation of the TGFBI dystrophies.
Title: Variant lattice corneal dystrophy associated with compound heterozygous mutations in the

Our findings demonstrate that granular corneal dystrophy type 1 may present with a vortex pattern of anterior stromal deposits. Although this pattern of dystrophic deposits is not recognized by clinicians as a typical phenotype of GCD1, it is consistent with the production of the majority of the TGFBI protein by the corneal epithelium.
Title: Vortex Pattern of Corneal Deposits in Granular Corneal Dystrophy Associated With the p.(Arg555Trp) Mutation in TGFBI.

ig-h3, Peroxiredoxin-2, and NRF2 have roles in cervical carcinogenesis
Title: Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis.

The right cornea showed a higher number of lattice lines than the left cornea. Molecular analysis involving bidirectional complete sequencing demonstrated a novel T621P mutation in exon 14 of TGFBI.
Title: Delayed Onset of Lattice Corneal Dystrophy Type IIIA Due to a Novel T621P Mutation in TGFBI.

TGFBIp expression is positively regulated by TGF-beta1 in corneal fibroblasts, whereas TGF-beta1-induced miR-21 and miR-181a negatively regulate TGFBIp expression.
Title: TGF-β regulates TGFBIp expression in corneal fibroblasts via miR-21, miR-181a, and Smad signaling.

The following TGFBI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGFBI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26539 NM_000358.2
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Homo sapiens transforming growth factor beta induced (TGFBI), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26539
Clone ID Related Accession (Same CDS sequence) NM_000358.2
Accession Version NM_000358.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-03
Organism Homo sapiens(Human)
Product transforming growth factor-beta-induced protein ig-h3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA051240.1, BC000097.2 and M77349.1. This sequence is a reference standard in the RefSeqGene project. On Mar 21, 2008 this sequence version replaced NM_000358.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000097.2, SRR1803614.146778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCGCTCT TCGTGCGGCT GCTGGCTCTC GCCCTGGCTC TGGCCCTGGG CCCCGCCGCG 
ACCCTGGCGG GTCCCGCCAA GTCGCCCTAC CAGCTGGTGC TGCAGCACAG CAGGCTCCGG
GGCCGCCAGC ACGGCCCCAA CGTGTGTGCT GTGCAGAAGG TTATTGGCAC TAATAGGAAG
TACTTCACCA ACTGCAAGCA GTGGTACCAA AGGAAAATCT GTGGCAAATC AACAGTCATC
AGCTACGAGT GCTGTCCTGG ATATGAAAAG GTCCCTGGGG AGAAGGGCTG TCCAGCAGCC
CTACCACTCT CAAACCTTTA CGAGACCCTG GGAGTCGTTG GATCCACCAC CACTCAGCTG
TACACGGACC GCACGGAGAA GCTGAGGCCT GAGATGGAGG GGCCCGGCAG CTTCACCATC
TTCGCCCCTA GCAACGAGGC CTGGGCCTCC TTGCCAGCTG AAGTGCTGGA CTCCCTGGTC
AGCAATGTCA ACATTGAGCT GCTCAATGCC CTCCGCTACC ATATGGTGGG CAGGCGAGTC
CTGACTGATG AGCTGAAACA CGGCATGACC CTCACCTCTA TGTACCAGAA TTCCAACATC
CAGATCCACC ACTATCCTAA TGGGATTGTA ACTGTGAACT GTGCCCGGCT GCTGAAAGCC
GACCACCATG CAACCAACGG GGTGGTGCAC CTCATCGATA AGGTCATCTC CACCATCACC
AACAACATCC AGCAGATCAT TGAGATCGAG GACACCTTTG AGACCCTTCG GGCTGCTGTG
GCTGCATCAG GGCTCAACAC GATGCTTGAA GGTAACGGCC AGTACACGCT TTTGGCCCCG
ACCAATGAGG CCTTCGAGAA GATCCCTAGT GAGACTTTGA ACCGTATCCT GGGCGACCCA
GAAGCCCTGA GAGACCTGCT GAACAACCAC ATCTTGAAGT CAGCTATGTG TGCTGAAGCC
ATCGTTGCGG GGCTGTCTGT AGAGACCCTG GAGGGCACGA CACTGGAGGT GGGCTGCAGC
GGGGACATGC TCACTATCAA CGGGAAGGCG ATCATCTCCA ATAAAGACAT CCTAGCCACC
AACGGGGTGA TCCACTACAT TGATGAGCTA CTCATCCCAG ACTCAGCCAA GACACTATTT
GAATTGGCTG CAGAGTCTGA TGTGTCCACA GCCATTGACC TTTTCAGACA AGCCGGCCTC
GGCAATCATC TCTCTGGAAG TGAGCGGTTG ACCCTCCTGG CTCCCCTGAA TTCTGTATTC
AAAGATGGAA CCCCTCCAAT TGATGCCCAT ACAAGGAATT TGCTTCGGAA CCACATAATT
AAAGACCAGC TGGCCTCTAA GTATCTGTAC CATGGACAGA CCCTGGAAAC TCTGGGCGGC
AAAAAACTGA GAGTTTTTGT TTATCGTAAT AGCCTCTGCA TTGAGAACAG CTGCATCGCG
GCCCACGACA AGAGGGGGAG GTACGGGACC CTGTTCACGA TGGACCGGGT GCTGACCCCC
CCAATGGGGA CTGTCATGGA TGTCCTGAAG GGAGACAATC GCTTTAGCAT GCTGGTAGCT
GCCATCCAGT CTGCAGGACT GACGGAGACC CTCAACCGGG AAGGAGTCTA CACAGTCTTT
GCTCCCACAA ATGAAGCCTT CCGAGCCCTG CCACCAAGAG AACGGAGCAG ACTCTTGGGA
GATGCCAAGG AACTTGCCAA CATCCTGAAA TACCACATTG GTGATGAAAT CCTGGTTAGC
GGAGGCATCG GGGCCCTGGT GCGGCTAAAG TCTCTCCAAG GTGACAAGCT GGAAGTCAGC
TTGAAAAACA ATGTGGTGAG TGTCAACAAG GAGCCTGTTG CCGAGCCTGA CATCATGGCC
ACAAATGGCG TGGTCCATGT CATCACCAAT GTTCTGCAGC CTCCAGCCAA CAGACCTCAG
GAAAGAGGGG ATGAACTTGC AGACTCTGCG CTTGAGATCT TCAAACAAGC ATCAGCGTTT
TCCAGGGCTT CCCAGAGGTC TGTGCGACTA GCCCCTGTCT ATCAAAAGTT ATTAGAGAGG
ATGAAGCATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000349.1
CDS162..2213
Misc Feature(1)132..134
Misc Feature(2)270..272
Misc Feature(3)354..356
Misc Feature(4)558..869
Misc Feature(5)918..1280
Misc Feature(6)1317..1661
Misc Feature(7)1701..2063
Misc Feature(8)2085..2093
Exon (1)1..295
Exon (2)296..394
Exon (3)395..459
Exon (4)460..620
Exon (5)621..785
Exon (6)786..932
Exon (7)933..1074
Exon (8)1075..1287
Exon (9)1288..1425
Exon (10)1426..1571
Exon (11)1572..1708
Exon (12)1709..1839
Exon (13)1840..1964
Exon (14)1965..2067
Exon (15)2068..2147
Exon (16)2148..2172
Exon (17)2173..2805
Translation

Target ORF information:

RefSeq Version NM_000358.2
Organism Homo sapiens(Human)
Definition Homo sapiens transforming growth factor beta induced (TGFBI), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000358.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCGCTCT TCGTGCGGCT GCTGGCTCTC GCCCTGGCTC TGGCCCTGGG CCCCGCCGCG 
ACCCTGGCGG GTCCCGCCAA GTCGCCCTAC CAGCTGGTGC TGCAGCACAG CAGGCTCCGG
GGCCGCCAGC ACGGCCCCAA CGTGTGTGCT GTGCAGAAGG TTATTGGCAC TAATAGGAAG
TACTTCACCA ACTGCAAGCA GTGGTACCAA AGGAAAATCT GTGGCAAATC AACAGTCATC
AGCTACGAGT GCTGTCCTGG ATATGAAAAG GTCCCTGGGG AGAAGGGCTG TCCAGCAGCC
CTACCACTCT CAAACCTTTA CGAGACCCTG GGAGTCGTTG GATCCACCAC CACTCAGCTG
TACACGGACC GCACGGAGAA GCTGAGGCCT GAGATGGAGG GGCCCGGCAG CTTCACCATC
TTCGCCCCTA GCAACGAGGC CTGGGCCTCC TTGCCAGCTG AAGTGCTGGA CTCCCTGGTC
AGCAATGTCA ACATTGAGCT GCTCAATGCC CTCCGCTACC ATATGGTGGG CAGGCGAGTC
CTGACTGATG AGCTGAAACA CGGCATGACC CTCACCTCTA TGTACCAGAA TTCCAACATC
CAGATCCACC ACTATCCTAA TGGGATTGTA ACTGTGAACT GTGCCCGGCT GCTGAAAGCC
GACCACCATG CAACCAACGG GGTGGTGCAC CTCATCGATA AGGTCATCTC CACCATCACC
AACAACATCC AGCAGATCAT TGAGATCGAG GACACCTTTG AGACCCTTCG GGCTGCTGTG
GCTGCATCAG GGCTCAACAC GATGCTTGAA GGTAACGGCC AGTACACGCT TTTGGCCCCG
ACCAATGAGG CCTTCGAGAA GATCCCTAGT GAGACTTTGA ACCGTATCCT GGGCGACCCA
GAAGCCCTGA GAGACCTGCT GAACAACCAC ATCTTGAAGT CAGCTATGTG TGCTGAAGCC
ATCGTTGCGG GGCTGTCTGT AGAGACCCTG GAGGGCACGA CACTGGAGGT GGGCTGCAGC
GGGGACATGC TCACTATCAA CGGGAAGGCG ATCATCTCCA ATAAAGACAT CCTAGCCACC
AACGGGGTGA TCCACTACAT TGATGAGCTA CTCATCCCAG ACTCAGCCAA GACACTATTT
GAATTGGCTG CAGAGTCTGA TGTGTCCACA GCCATTGACC TTTTCAGACA AGCCGGCCTC
GGCAATCATC TCTCTGGAAG TGAGCGGTTG ACCCTCCTGG CTCCCCTGAA TTCTGTATTC
AAAGATGGAA CCCCTCCAAT TGATGCCCAT ACAAGGAATT TGCTTCGGAA CCACATAATT
AAAGACCAGC TGGCCTCTAA GTATCTGTAC CATGGACAGA CCCTGGAAAC TCTGGGCGGC
AAAAAACTGA GAGTTTTTGT TTATCGTAAT AGCCTCTGCA TTGAGAACAG CTGCATCGCG
GCCCACGACA AGAGGGGGAG GTACGGGACC CTGTTCACGA TGGACCGGGT GCTGACCCCC
CCAATGGGGA CTGTCATGGA TGTCCTGAAG GGAGACAATC GCTTTAGCAT GCTGGTAGCT
GCCATCCAGT CTGCAGGACT GACGGAGACC CTCAACCGGG AAGGAGTCTA CACAGTCTTT
GCTCCCACAA ATGAAGCCTT CCGAGCCCTG CCACCAAGAG AACGGAGCAG ACTCTTGGGA
GATGCCAAGG AACTTGCCAA CATCCTGAAA TACCACATTG GTGATGAAAT CCTGGTTAGC
GGAGGCATCG GGGCCCTGGT GCGGCTAAAG TCTCTCCAAG GTGACAAGCT GGAAGTCAGC
TTGAAAAACA ATGTGGTGAG TGTCAACAAG GAGCCTGTTG CCGAGCCTGA CATCATGGCC
ACAAATGGCG TGGTCCATGT CATCACCAAT GTTCTGCAGC CTCCAGCCAA CAGACCTCAG
GAAAGAGGGG ATGAACTTGC AGACTCTGCG CTTGAGATCT TCAAACAAGC ATCAGCGTTT
TCCAGGGCTT CCCAGAGGTC TGTGCGACTA GCCCCTGTCT ATCAAAAGTT ATTAGAGAGG
ATGAAGCATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis.
BioMed research international20174180703(2017)
Kontostathi G,Zoidakis J,Makridakis M,Lygirou V,Mermelekas G,Papadopoulos T,Vougas K,Vlamis-Gardikas A,Drakakis P,Loutradis D,Vlahou A,Anagnou NP,Pappa KI


book

Delayed Onset of Lattice Corneal Dystrophy Type IIIA Due to a Novel T621P Mutation in TGFBI.
Journal of refractive surgery (Thorofare, N.J. : 1995)32(5)356(2016 May)
Lee J,Ji YW,Park SY,Seo KY,Kim TI,Kim EK


book

TGF-β regulates TGFBIp expression in corneal fibroblasts via miR-21, miR-181a, and Smad signaling.
Biochemical and biophysical research communications472(1)150-5(2016 Mar)
Choi SI,Jin JY,Maeng YS,Kim TI,Kim EK


book

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M


book

LASIK surgery of granular corneal dystrophy type 2 patients leads to accumulation and differential proteolytic processing of transforming growth factor beta-induced protein (TGFBIp).
Proteomics16(3)539-43(2016 Feb)
Poulsen ET,Nielsen NS,Jensen MM,Nielsen E,Hjortdal J,Kim EK,Enghild JJ


 
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