• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

NKX2-1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol NKX2-1
Entrez Gene ID 7080
Full Name NK2 homeobox 1
Synonyms BCH,BHC,NK-2,NKX2.1,NKX2A,NMTC1,T/EBP,TEBP,TITF1,TTF-1,TTF1
General protein information
Preferred Names

NK2 homeobox 1

Names

homeobox protein Nkx-2.1
NK-2 homolog A
homeobox protein NK-2 homolog A
thyroid nuclear factor 1
thyroid transcription factor 1
thyroid-specific enhancer-binding protein
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

14

14q13.3
Summary This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014].
Disorder MIM:

600635

Disorder Html: {Thyroid cancer, nonmedullary, 1}, 188550 (3); Chorea, hereditary benign, 118700 (3); Choreoathetosis, hypothyroidism, and neonatal respiratory distress, 610978 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001079668.3 NP_001073136.1 homeobox protein Nkx-2.1 isoform 1
NM_001079668.2 NP_001073136.1 homeobox protein Nkx-2.1 isoform 1
NM_003317.4 NP_003308.1 homeobox protein Nkx-2.1 isoform 2
NM_003317.3 NP_003308.1 homeobox protein Nkx-2.1 isoform 2

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Nkx2-1 NP_037225.1
Danio rerio (zebrafish) nkx2.1b NP_571851.1
Xenopus tropicalis (tropical clawed frog) nkx2-1 XP_002935383.1
Homo sapiens (human) NKX2-1 NP_001073136.1
Pan troglodytes (chimpanzee) NKX2-1 XP_509911.2
NKX2-1 XP_001089773.1
Bos taurus (cattle) LOC613713 NP_001178387.1
Mus musculus (house mouse) Nkx2-1 NP_001139670.1
Gallus gallus (chicken) NKX2-1 NP_989947.1

Gene Ontology

The following NKX2-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKX2-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16274 NM_003317.4
Latest version!		 Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu22673 NM_001079668.3
Latest version!		 Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
View Old Accession Versions >>
OHu22673 NM_001079668.2 Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu16274 NM_003317.3 Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu16274
Clone ID Related Accession (Same CDS sequence) NM_003317.4 , NM_003317.3
Accession Version NM_003317.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-27
Organism Homo sapiens(human)
Product homeobox protein Nkx-2.1 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U33749.1, BC006221.2 and BM970838.1. On Jun 2, 2019 this sequence version replaced NM_003317.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279835.7804.1, DRR138524.267689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG 
TACAGGCAGG GCCAGGCGGC ACCGCCAACA GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGAGGAACAG CGCCTCTGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC AAGCGCGCCG 
CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG GCGCAGGTGT ACGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC GGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACGCCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAAATGAA GCGCCAGGCC 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG GGGCGGCGGG 
GGCACCGGGT GCCCGCAGCA GCAACAGGCT CAGCAGCAGT CGCCGCGACG CGTGGCGGTG 
CCGGTCCTGG TGAAAGACGG CAAACCGTGC CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC 
AGCCTACAAG GCCACGCGCA GCAGCAGGCG CAGCACCAGG CGCAGGCCGC GCAGGCGGCG 
GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC GCCGGCCTTG GCGCACACCC GGGCCACCAG 
CCAGGCAGCG CAGGCCAGTC TCCGGACCTG GCGCACCACG CCGCCAGCCC CGCGGCGCTG 
CAGGGCCAGG TATCCAGCCT GTCCCACCTG AACTCCTCGG GCTCGGACTA CGGCACCATG 
TCCTGCTCCA CCTTGCTATA CGGTCGGACC TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003308.1
CDS200..1315
Translation

Target ORF information:

RefSeq Version NM_003317.4
Organism Homo sapiens(human)
Definition Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003317.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG 
TACAGGCAGG GCCAGGCGGC ACCGCCAACA GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGAGGAACAG CGCCTCTGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC AAGCGCGCCG 
CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG GCGCAGGTGT ACGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC GGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACGCCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAAATGAA GCGCCAGGCC 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG GGGCGGCGGG 
GGCACCGGGT GCCCGCAGCA GCAACAGGCT CAGCAGCAGT CGCCGCGACG CGTGGCGGTG 
CCGGTCCTGG TGAAAGACGG CAAACCGTGC CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC 
AGCCTACAAG GCCACGCGCA GCAGCAGGCG CAGCACCAGG CGCAGGCCGC GCAGGCGGCG 
GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC GCCGGCCTTG GCGCACACCC GGGCCACCAG 
CCAGGCAGCG CAGGCCAGTC TCCGGACCTG GCGCACCACG CCGCCAGCCC CGCGGCGCTG 
CAGGGCCAGG TATCCAGCCT GTCCCACCTG AACTCCTCGG GCTCGGACTA CGGCACCATG 
TCCTGCTCCA CCTTGCTATA CGGTCGGACC TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16274
Clone ID Related Accession (Same CDS sequence) NM_003317.4 , NM_003317.3
Accession Version NM_003317.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-03-11
Organism Homo sapiens(human)
Product homeobox protein Nkx-2.1 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U33749.1, BC006221.2 and BM970838.1. On or before Jul 8, 2009 this sequence version replaced XM_002344771.1, NM_003317.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U33749.1, ERR279873.1315.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG 
TACAGGCAGG GCCAGGCGGC ACCGCCAACA GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGAGGAACAG CGCCTCTGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC AAGCGCGCCG 
CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG GCGCAGGTGT ACGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC GGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACGCCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAAATGAA GCGCCAGGCC 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG GGGCGGCGGG 
GGCACCGGGT GCCCGCAGCA GCAACAGGCT CAGCAGCAGT CGCCGCGACG CGTGGCGGTG 
CCGGTCCTGG TGAAAGACGG CAAACCGTGC CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC 
AGCCTACAAG GCCACGCGCA GCAGCAGGCG CAGCACCAGG CGCAGGCCGC GCAGGCGGCG 
GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC GCCGGCCTTG GCGCACACCC GGGCCACCAG 
CCAGGCAGCG CAGGCCAGTC TCCGGACCTG GCGCACCACG CCGCCAGCCC CGCGGCGCTG 
CAGGGCCAGG TATCCAGCCT GTCCCACCTG AACTCCTCGG GCTCGGACTA CGGCACCATG 
TCCTGCTCCA CCTTGCTATA CGGTCGGACC TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003308.1
CDS342..1457
Translation

Target ORF information:

RefSeq Version NM_003317.3
Organism Homo sapiens(human)
Definition Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003317.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGTCGATGA GTCCAAAGCA CACGACTCCG TTCTCAGTGT CTGACATCTT GAGTCCCCTG 
GAGGAAAGCT ACAAGAAAGT GGGCATGGAG GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG 
TACAGGCAGG GCCAGGCGGC ACCGCCAACA GCGGCCATGC AGCAGCACGC CGTGGGGCAC 
CACGGCGCCG TCACCGCCGC CTACCACATG ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC 
TCCGCCGTGG GGGGCTACTG CAACGGCAAC CTGGGCAACA TGAGCGAGCT GCCGCCGTAC 
CAGGACACCA TGAGGAACAG CGCCTCTGGC CCCGGATGGT ACGGCGCCAA CCCAGACCCG 
CGCTTCCCCG CCATCTCCCG CTTCATGGGC CCGGCGAGCG GCATGAACAT GAGCGGCATG 
GGCGGCCTGG GCTCGCTGGG GGACGTGAGC AAGAACATGG CCCCGCTGCC AAGCGCGCCG 
CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG GCGCAGGTGT ACGAGCTGGA GCGACGCTTC 
AAGCAACAGA AGTACCTGTC GGCGCCGGAG CGCGAGCACC TGGCCAGCAT GATCCACCTG 
ACGCCCACGC AGGTCAAGAT CTGGTTCCAG AACCACCGCT ACAAAATGAA GCGCCAGGCC 
AAGGACAAGG CGGCGCAGCA GCAACTGCAG CAGGACAGCG GCGGCGGCGG GGGCGGCGGG 
GGCACCGGGT GCCCGCAGCA GCAACAGGCT CAGCAGCAGT CGCCGCGACG CGTGGCGGTG 
CCGGTCCTGG TGAAAGACGG CAAACCGTGC CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC 
AGCCTACAAG GCCACGCGCA GCAGCAGGCG CAGCACCAGG CGCAGGCCGC GCAGGCGGCG 
GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC GCCGGCCTTG GCGCACACCC GGGCCACCAG 
CCAGGCAGCG CAGGCCAGTC TCCGGACCTG GCGCACCACG CCGCCAGCCC CGCGGCGCTG 
CAGGGCCAGG TATCCAGCCT GTCCCACCTG AACTCCTCGG GCTCGGACTA CGGCACCATG 
TCCTGCTCCA CCTTGCTATA CGGTCGGACC TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22673
Clone ID Related Accession (Same CDS sequence) NM_001079668.3 , NM_001079668.2
Accession Version NM_001079668.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1206bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-27
Organism Homo sapiens(human)
Product homeobox protein Nkx-2.1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL132857.4, U43203.1 and BM970287.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001079668.2. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006221.2, U43203.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
AGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG TACAGGCAGG GCCAGGCGGC ACCGCCAACA 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCCG TCACCGCCGC CTACCACATG 
ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGAGGAACAG CGCCTCTGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCGCTGGG GGACGTGAGC 
AAGAACATGG CCCCGCTGCC AAGCGCGCCG CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG 
GCGCAGGTGT ACGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC GGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACGCCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAAATGAA GCGCCAGGCC AAGGACAAGG CGGCGCAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG GGGCGGCGGG GGCACCGGGT GCCCGCAGCA GCAACAGGCT 
CAGCAGCAGT CGCCGCGACG CGTGGCGGTG CCGGTCCTGG TGAAAGACGG CAAACCGTGC 
CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC AGCCTACAAG GCCACGCGCA GCAGCAGGCG 
CAGCACCAGG CGCAGGCCGC GCAGGCGGCG GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC 
GCCGGCCTTG GCGCACACCC GGGCCACCAG CCAGGCAGCG CAGGCCAGTC TCCGGACCTG 
GCGCACCACG CCGCCAGCCC CGCGGCGCTG CAGGGCCAGG TATCCAGCCT GTCCCACCTG 
AACTCCTCGG GCTCGGACTA CGGCACCATG TCCTGCTCCA CCTTGCTATA CGGTCGGACC 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001073136.1
CDS104..1309
Translation

Target ORF information:

RefSeq Version NM_001079668.3
Organism Homo sapiens(human)
Definition Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001079668.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
AGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG TACAGGCAGG GCCAGGCGGC ACCGCCAACA 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCCG TCACCGCCGC CTACCACATG 
ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGAGGAACAG CGCCTCTGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCGCTGGG GGACGTGAGC 
AAGAACATGG CCCCGCTGCC AAGCGCGCCG CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG 
GCGCAGGTGT ACGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC GGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACGCCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAAATGAA GCGCCAGGCC AAGGACAAGG CGGCGCAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG GGGCGGCGGG GGCACCGGGT GCCCGCAGCA GCAACAGGCT 
CAGCAGCAGT CGCCGCGACG CGTGGCGGTG CCGGTCCTGG TGAAAGACGG CAAACCGTGC 
CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC AGCCTACAAG GCCACGCGCA GCAGCAGGCG 
CAGCACCAGG CGCAGGCCGC GCAGGCGGCG GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC 
GCCGGCCTTG GCGCACACCC GGGCCACCAG CCAGGCAGCG CAGGCCAGTC TCCGGACCTG 
GCGCACCACG CCGCCAGCCC CGCGGCGCTG CAGGGCCAGG TATCCAGCCT GTCCCACCTG 
AACTCCTCGG GCTCGGACTA CGGCACCATG TCCTGCTCCA CCTTGCTATA CGGTCGGACC 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22673
Clone ID Related Accession (Same CDS sequence) NM_001079668.3 , NM_001079668.2
Accession Version NM_001079668.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1206bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-03-11
Organism Homo sapiens(human)
Product homeobox protein Nkx-2.1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL132857.4, U43203.1 and BM970287.1. This sequence is a reference standard in the RefSeqGene project. On Oct 10, 2009 this sequence version replaced NM_001079668.1. Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U43203.1, BT009773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2155770 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
AGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG TACAGGCAGG GCCAGGCGGC ACCGCCAACA 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCCG TCACCGCCGC CTACCACATG 
ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGAGGAACAG CGCCTCTGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCGCTGGG GGACGTGAGC 
AAGAACATGG CCCCGCTGCC AAGCGCGCCG CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG 
GCGCAGGTGT ACGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC GGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACGCCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAAATGAA GCGCCAGGCC AAGGACAAGG CGGCGCAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG GGGCGGCGGG GGCACCGGGT GCCCGCAGCA GCAACAGGCT 
CAGCAGCAGT CGCCGCGACG CGTGGCGGTG CCGGTCCTGG TGAAAGACGG CAAACCGTGC 
CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC AGCCTACAAG GCCACGCGCA GCAGCAGGCG 
CAGCACCAGG CGCAGGCCGC GCAGGCGGCG GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC 
GCCGGCCTTG GCGCACACCC GGGCCACCAG CCAGGCAGCG CAGGCCAGTC TCCGGACCTG 
GCGCACCACG CCGCCAGCCC CGCGGCGCTG CAGGGCCAGG TATCCAGCCT GTCCCACCTG 
AACTCCTCGG GCTCGGACTA CGGCACCATG TCCTGCTCCA CCTTGCTATA CGGTCGGACC 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001073136.1
CDS97..1302
Translation

Target ORF information:

RefSeq Version NM_001079668.2
Organism Homo sapiens(human)
Definition Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001079668.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTGGTCCG GAGGCAGTGG GAAGGCGCGG GGCTGGGAGG CCGCGGCGGG AGGGAGGAGC 
AGCCCCGGCA GGCTCAGCCG CCGCCGAATC ATGTCGATGA GTCCAAAGCA CACGACTCCG 
TTCTCAGTGT CTGACATCTT GAGTCCCCTG GAGGAAAGCT ACAAGAAAGT GGGCATGGAG 
GGCGGCGGCC TCGGGGCTCC GCTGGCGGCG TACAGGCAGG GCCAGGCGGC ACCGCCAACA 
GCGGCCATGC AGCAGCACGC CGTGGGGCAC CACGGCGCCG TCACCGCCGC CTACCACATG 
ACGGCGGCGG GGGTGCCCCA GCTCTCGCAC TCCGCCGTGG GGGGCTACTG CAACGGCAAC 
CTGGGCAACA TGAGCGAGCT GCCGCCGTAC CAGGACACCA TGAGGAACAG CGCCTCTGGC 
CCCGGATGGT ACGGCGCCAA CCCAGACCCG CGCTTCCCCG CCATCTCCCG CTTCATGGGC 
CCGGCGAGCG GCATGAACAT GAGCGGCATG GGCGGCCTGG GCTCGCTGGG GGACGTGAGC 
AAGAACATGG CCCCGCTGCC AAGCGCGCCG CGCAGGAAGC GCCGGGTGCT CTTCTCGCAG 
GCGCAGGTGT ACGAGCTGGA GCGACGCTTC AAGCAACAGA AGTACCTGTC GGCGCCGGAG 
CGCGAGCACC TGGCCAGCAT GATCCACCTG ACGCCCACGC AGGTCAAGAT CTGGTTCCAG 
AACCACCGCT ACAAAATGAA GCGCCAGGCC AAGGACAAGG CGGCGCAGCA GCAACTGCAG 
CAGGACAGCG GCGGCGGCGG GGGCGGCGGG GGCACCGGGT GCCCGCAGCA GCAACAGGCT 
CAGCAGCAGT CGCCGCGACG CGTGGCGGTG CCGGTCCTGG TGAAAGACGG CAAACCGTGC 
CAGGCGGGTG CCCCCGCGCC GGGCGCCGCC AGCCTACAAG GCCACGCGCA GCAGCAGGCG 
CAGCACCAGG CGCAGGCCGC GCAGGCGGCG GCAGCGGCCA TCTCCGTGGG CAGCGGTGGC 
GCCGGCCTTG GCGCACACCC GGGCCACCAG CCAGGCAGCG CAGGCCAGTC TCCGGACCTG 
GCGCACCACG CCGCCAGCCC CGCGGCGCTG CAGGGCCAGG TATCCAGCCT GTCCCACCTG 
AACTCCTCGG GCTCGGACTA CGGCACCATG TCCTGCTCCA CCTTGCTATA CGGTCGGACC 
TGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed