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TNNI3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TNNI3
Entrez Gene ID 7137
Full Name troponin I3, cardiac type
Synonyms CMD1FF,CMD2A,CMH7,RCM1,TNNC1,cTnI
General protein information
Preferred Names

troponin I3, cardiac type

Names

troponin I, cardiac muscle
cardiomyopathy, dilated 2A (autosomal recessive)
troponin I type 3 (cardiac)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19q13.42

Summary Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008].
Disorder MIM:

191044

Disorder Html: Cardiomyopathy, familial hypertrophic, 7, 613690 (3); Cardiomyopathy,

mRNA and Protein(s)

mRNA Protein Name
NM_000363.4 NP_000354.4 troponin I, cardiac muscle



Macaca mulatta (rhesus monkey) TNNI3 XP_001085820.1
Mus musculus (house mouse) Tnni3 NP_033432.1
Rattus norvegicus (Norway rat) Tnni3 NP_058840.1
Pan troglodytes (chimpanzee) TNNI3 XP_001134934.2
Bos taurus (cattle) LOC511094 NP_001035607.1
Xenopus tropicalis (tropical clawed frog) tnni3 NP_001011410.1
Homo sapiens (human) TNNI3 NP_000354.4
Canis lupus familiaris (dog) TNNI3 NP_001003041.1


Related articles in PubMed

High-sensitivity cardiac troponin I and NT-proBNP as predictors of incident dementia and Alzheimer's disease: the FINRISK Study.
Tynkkynen J, Hernesniemi JA, Laatikainen T, Havulinna AS, Salo P, Blankenberg S, Zeller T, Salomaa V
Journal of neurology264(3)503-511(2017 Mar)

Factors independently associated with cardiac troponin I levels in young and healthy adults from the general population.
Bossard M, Thériault S, Aeschbacher S, Schoen T, Kunz S, von Rotz M, Estis J, Todd J, Risch M, Mueller C, Risch L, Paré G, Conen D
Clinical research in cardiology : official journal of the German Cardiac Society106(2)96-104(2017 Feb)

Antihuman Cardiac Troponin I (hcTn I) Monoclonal Antibody.

Analysis of the Indicating Value of Cardiac Troponin I, Tumor Necrosis Factor-α, Interleukin-18, Mir-1 and Mir-146b for Viral Myocarditis among Children.
Wang D, Li T, Cui H, Zhang Y
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(6)1325-1333(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Plasma troponin C1 (cTnI) is biomarker of choice for diagnosing acute myocardial infarction (AMI) because of its high specificity as biomarker for damage to myocardial tissue. Data suggest the "best cut-off" for plasma cTnI is 0.014 micrograms/L in AMI. These studies were conducted in emergency department of a university hospital in Italy using point-of-care testing in patients presenting with chest pain, ages 18-101.
Title: Clinical performance of cardiac Troponin I: A comparison between the POCT AQT90 FLEX and the Dimension Vista analyzer in an emergency setting.

Serial measurement of troponin I revealed a persistent elevation in patients with diabetes mellitus type 2.
Title: Serial Measurement of High-Sensitivity Troponin I and Cardiovascular Outcomes in Patients With Type 2 Diabetes Mellitus in the EXAMINE Trial (Examination of Cardiovascular Outcomes With Alogliptin Versus Standard of Care).

Study found that N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-sensitivity cardiac troponin I are independently associated with incident dementia and NT-proBNP with incident Alzheimer's disease
Title: High-sensitivity cardiac troponin I and NT-proBNP as predictors of incident dementia and Alzheimer's disease: the FINRISK Study.

value of cTnI level assessed 24 hours post-surgery was a reliable predictor of death following liver transplantation with optimal cut-off value of 0.215 ng/mL. The surgery time was the most important predictor of cTnI elevation.
Title: Prognostic value of perioperative assessment of plasma cardiac troponin I in patients undergoing liver transplantation.

83 preterm infants with Bronchopulmonary dysplasia born <28-wk gestation and still inpatients at 36-wk corrected age received an echocardiogram and blood tests of B-type natriuretic peptide (BNP), troponin I, and YKL-40.
Title: BNP, troponin I, and YKL-40 as screening markers in extremely preterm infants at risk for pulmonary hypertension associated with bronchopulmonary dysplasia.

The following TNNI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25717 NM_000363.4
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Homo sapiens troponin I3, cardiac type (TNNI3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25717
Clone ID Related Accession (Same CDS sequence) NM_000363.4
Accession Version NM_000363.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 633bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product troponin I, cardiac muscle
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK223451.1. This sequence is a reference standard in the RefSeqGene project. On Jul 5, 2007 this sequence version replaced NM_000363.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223451.1, EU747720.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGGATG GGAGCAGCGA TGCGGCTAGG GAACCTCGCC CTGCACCAGC CCCAATCAGA 
CGCCGCTCCT CCAACTACCG CGCTTATGCC ACGGAGCCGC ACGCCAAGAA AAAATCTAAG
ATCTCCGCCT CGAGAAAATT GCAGCTGAAG ACTCTGCTGC TGCAGATTGC AAAGCAAGAG
CTGGAGCGAG AGGCGGAGGA GCGGCGCGGA GAGAAGGGGC GCGCTCTGAG CACCCGCTGC
CAGCCGCTGG AGTTGGCCGG GCTGGGCTTC GCGGAGCTGC AGGACTTGTG CCGACAGCTC
CACGCCCGTG TGGACAAGGT GGATGAAGAG AGATACGACA TAGAGGCAAA AGTCACCAAG
AACATCACGG AGATTGCAGA TCTGACTCAG AAGATCTTTG ACCTTCGAGG CAAGTTTAAG
CGGCCCACCC TGCGGAGAGT GAGGATCTCT GCAGATGCCA TGATGCAGGC GCTGCTGGGG
GCCCGGGCTA AGGAGTCCCT GGACCTGCGG GCCCACCTCA AGCAGGTGAA GAAGGAGGAC
ACCGAGAAGG AAAACCGGGA GGTGGGAGAC TGGCGCAAGA ACATCGATGC ACTGAGTGGA
ATGGAGGGCC GCAAGAAAAA GTTTGAGAGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000354.4
CDS144..776
Misc Feature(1)144..236
Misc Feature(2)147..149
Misc Feature(3)156..158
Misc Feature(4)159..161
Misc Feature(5)210..212
Misc Feature(6)213..215
Misc Feature(7)219..221
Misc Feature(8)234..236
Misc Feature(9)237..380
Misc Feature(10)267..269
Misc Feature(11)273..275
Misc Feature(12)294..296
Misc Feature(13)357..674
Misc Feature(14)372..374
Misc Feature(15)375..377
Misc Feature(16)381..383
Misc Feature(17)432..434
Misc Feature(18)528..590
Misc Feature(19)528..530
Misc Feature(20)570..572
Misc Feature(21)591..593
Misc Feature(22)639..641
Misc Feature(23)684..686
Misc Feature(24)738..740
Exon (1)1..154
Exon (2)155..167
Exon (3)168..251
Exon (4)252..293
Exon (5)294..425
Exon (6)426..515
Exon (7)516..692
Exon (8)693..843
Translation

Target ORF information:

RefSeq Version NM_000363.4
Organism Homo sapiens(Human)
Definition Homo sapiens troponin I3, cardiac type (TNNI3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000363.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGGATG GGAGCAGCGA TGCGGCTAGG GAACCTCGCC CTGCACCAGC CCCAATCAGA 
CGCCGCTCCT CCAACTACCG CGCTTATGCC ACGGAGCCGC ACGCCAAGAA AAAATCTAAG
ATCTCCGCCT CGAGAAAATT GCAGCTGAAG ACTCTGCTGC TGCAGATTGC AAAGCAAGAG
CTGGAGCGAG AGGCGGAGGA GCGGCGCGGA GAGAAGGGGC GCGCTCTGAG CACCCGCTGC
CAGCCGCTGG AGTTGGCCGG GCTGGGCTTC GCGGAGCTGC AGGACTTGTG CCGACAGCTC
CACGCCCGTG TGGACAAGGT GGATGAAGAG AGATACGACA TAGAGGCAAA AGTCACCAAG
AACATCACGG AGATTGCAGA TCTGACTCAG AAGATCTTTG ACCTTCGAGG CAAGTTTAAG
CGGCCCACCC TGCGGAGAGT GAGGATCTCT GCAGATGCCA TGATGCAGGC GCTGCTGGGG
GCCCGGGCTA AGGAGTCCCT GGACCTGCGG GCCCACCTCA AGCAGGTGAA GAAGGAGGAC
ACCGAGAAGG AAAACCGGGA GGTGGGAGAC TGGCGCAAGA ACATCGATGC ACTGAGTGGA
ATGGAGGGCC GCAAGAAAAA GTTTGAGAGC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

High-sensitivity cardiac troponin I and NT-proBNP as predictors of incident dementia and Alzheimer's disease: the FINRISK Study.
Journal of neurology264(3)503-511(2017 Mar)
Tynkkynen J,Hernesniemi JA,Laatikainen T,Havulinna AS,Salo P,Blankenberg S,Zeller T,Salomaa V


book

Factors independently associated with cardiac troponin I levels in young and healthy adults from the general population.
Clinical research in cardiology : official journal of the German Cardiac Society106(2)96-104(2017 Feb)
Bossard M,Thériault S,Aeschbacher S,Schoen T,Kunz S,von Rotz M,Estis J,Todd J,Risch M,Mueller C,Risch L,Paré G,Conen D


book

Antihuman Cardiac Troponin I (hcTn I) Monoclonal Antibody.
Monoclonal antibodies in immunodiagnosis and immunotherapy35(4)236(2016 Aug)


book

Analysis of the Indicating Value of Cardiac Troponin I, Tumor Necrosis Factor-α, Interleukin-18, Mir-1 and Mir-146b for Viral Myocarditis among Children.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology40(6)1325-1333(2016)
Wang D,Li T,Cui H,Zhang Y


 
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