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TNNT2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol TNNT2
Entrez Gene ID 7139
Full Name troponin T type 2 (cardiac)
Synonyms CMD1D, CMH2, CMPD2, LVNC6, RCM3, TnTC, cTnT
General protein information
Preferred Names
troponin T, cardiac muscle
Names
troponin T, cardiac muscle
troponin T2, cardiac
cardiomyopathy, hypertrophic 2
cardiomyopathy, dilated 1D (autosomal dominant)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1q32

Summary The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

191045

Disorder Html: Cardiomyopathy, familial hypertrophic, 2, 115195 (3); Cardiomyopathy,

mRNA and Protein(s)

mRNA Protein Name
NM_000364 NP_000355 troponin T, cardiac muscle isoform 1
NM_001276347 NP_001263276 troponin T, cardiac muscle isoform 2
NM_001276346 NP_001263275 troponin T, cardiac muscle isoform 6
NM_001276345 NP_001263274 troponin T, cardiac muscle isoform 5
NM_001001432 NP_001001432 troponin T, cardiac muscle isoform 4
NM_001001430 NP_001001430 troponin T, cardiac muscle isoform 2
NM_001001431 NP_001001431 troponin T, cardiac muscle isoform 3
XM_011509938 XP_011508240 troponin T, cardiac muscle isoform X2
XM_011509939 XP_011508241 troponin T, cardiac muscle isoform X3
XM_011509940 XP_011508242 troponin T, cardiac muscle isoform X4
XM_011509941 XP_011508243 troponin T, cardiac muscle isoform X5
XM_006711508 XP_006711571 troponin T, cardiac muscle isoform X1
XM_006711509 XP_006711572 troponin T, cardiac muscle isoform X6
XM_011509942 XP_011508244 troponin T, cardiac muscle isoform X7
XM_011509943 XP_011508245 troponin T, cardiac muscle isoform X7
XM_011509944 XP_011508246 troponin T, cardiac muscle isoform X8
XM_011509945 XP_011508247 troponin T, cardiac muscle isoform X9
XM_011509946 XP_011508248 troponin T, cardiac muscle isoform X10



Homo sapiens (human) TNNT2 NP_001001432.1
Pan troglodytes (chimpanzee) TNNT2 XP_001145598.1
Macaca mulatta (Rhesus monkey) TNNT2 XP_002802032.1
Canis lupus familiaris (dog) TNNT2 NP_001003012.1
Bos taurus (cattle) TNNT2 NP_777196.1
Mus musculus (house mouse) Tnnt2 NP_001123650.1
Rattus norvegicus (Norway rat) Tnnt2 NP_036808.1
Gallus gallus (chicken) TNNT2 NP_990780.1
Danio rerio (zebrafish) tnnt2a NP_690853.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC100493699 XP_004919786.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TNNT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu24970
NM_000364 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu24695
NM_001276347 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25008 NM_001276346 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu25054 NM_001276345 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu24894 NM_001001432 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu24695
NM_001001430 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu24914
NM_001001431 Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25054 XM_011509938 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61071 XM_011509939 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61072 XM_011509940 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61073 XM_011509941 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu24695
XM_006711508 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu34665 XM_006711509 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu34668 XM_011509942 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu34668 XM_011509943 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu34669 XM_011509944 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61074 XM_011509945 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu61075 XM_011509946 PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24970
Clone ID Related Accession (Same CDS sequence) NM_000364
Accession Version NM_000364.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79855.1, BC002653.2 and AA865519.1. On Feb 5, 2013 this sequence version replaced gi:48255876. Transcript Variant: This variant (1) lacks an in-frame exon in the 3' coding region, compared to variant 5. It encodes a shorter isoform (1) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000355.2
CDS93..980
Misc Feature(1)75..77(+)
Misc Feature(2)96..98(+)
Misc Feature(3)96..98(+)
Misc Feature(4)399..782(+)
Misc Feature(5)693..695(+)
Misc Feature(6)693..695(+)
Misc Feature(7)705..707(+)
Misc Feature(8)705..707(+)
Misc Feature(9)720..722(+)
Misc Feature(10)720..722(+)
Misc Feature(11)963..965(+)
Misc Feature(12)963..965(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..189
Gene:TNNT2
Gene Synonym:
Exon (6)190..255
Gene:TNNT2
Gene Synonym:
Exon (7)256..291
Gene:TNNT2
Gene Synonym:
Exon (8)292..325
Gene:TNNT2
Gene Synonym:
Exon (9)326..386
Gene:TNNT2
Gene Synonym:
Exon (10)387..503
Gene:TNNT2
Gene Synonym:
Exon (11)504..581
Gene:TNNT2
Gene Synonym:
Exon (12)582..692
Gene:TNNT2
Gene Synonym:
Exon (13)693..802
Gene:TNNT2
Gene Synonym:
Exon (14)803..893
Gene:TNNT2
Gene Synonym:
Exon (15)894..934
Gene:TNNT2
Gene Synonym:
Exon (16)935..1182
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000364
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000364

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24695
Clone ID Related Accession (Same CDS sequence) NM_001001430 , NM_001276347 , XM_006711508
Accession Version NM_001276347.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290621.1, BC002653.2 and AA865519.1. Transcript Variant: This variant (7) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region compared to variant 5. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 5. Variants 2 and 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263276.1
CDS226..1092
Misc Feature(1)190..192(+)
Misc Feature(2)502..894(+)
Misc Feature(3)793..>1035(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..266
Gene:TNNT2
Gene Synonym:
Exon (3)267..277
Gene:TNNT2
Gene Synonym:
Exon (4)278..292
Gene:TNNT2
Gene Synonym:
Exon (5)293..358
Gene:TNNT2
Gene Synonym:
Exon (6)359..394
Gene:TNNT2
Gene Synonym:
Exon (7)395..428
Gene:TNNT2
Gene Synonym:
Exon (8)429..489
Gene:TNNT2
Gene Synonym:
Exon (9)490..606
Gene:TNNT2
Gene Synonym:
Exon (10)607..684
Gene:TNNT2
Gene Synonym:
Exon (11)685..795
Gene:TNNT2
Gene Synonym:
Exon (12)796..804
Gene:TNNT2
Gene Synonym:
Exon (13)805..914
Gene:TNNT2
Gene Synonym:
Exon (14)915..1005
Gene:TNNT2
Gene Synonym:
Exon (15)1006..1046
Gene:TNNT2
Gene Synonym:
Exon (16)1047..1294
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276347
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276347

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25008
Clone ID Related Accession (Same CDS sequence) NM_001276346
Accession Version NM_001276346.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 768bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79857.1 and AA865519.1. Transcript Variant: This variant (6) lacks multiple in-frame exons and uses an alternate in-frame splice site in the 5' coding region, compared to variant 5. It encodes a shorter isoform (6) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263275.1
CDS93..860
Misc Feature(1)75..77(+)
Misc Feature(2)603..>803(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..189
Gene:TNNT2
Gene Synonym:
Exon (6)190..255
Gene:TNNT2
Gene Synonym:
Exon (7)256..288
Gene:TNNT2
Gene Synonym:
Exon (8)289..322
Gene:TNNT2
Gene Synonym:
Exon (9)323..383
Gene:TNNT2
Gene Synonym:
Exon (10)384..461
Gene:TNNT2
Gene Synonym:
Exon (11)462..572
Gene:TNNT2
Gene Synonym:
Exon (12)573..682
Gene:TNNT2
Gene Synonym:
Exon (13)683..773
Gene:TNNT2
Gene Synonym:
Exon (14)774..814
Gene:TNNT2
Gene Synonym:
Exon (15)815..1062
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276346
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276346

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
ACAGAGCGGA AAAGTGGGAA GAGGCAGACT GAGCGGGAAA AGAAGAAGAA GATTCTGGCT
GAGAGGAGGA AGGTGCTGGC CATTGACCAC CTGAATGAAG ATCAGCTGAG GGAGAAGGCC
AAGGAGCTGT GGCAGAGCAT CTATAACTTG GAGGCAGAGA AGTTCGACCT GCAGGAGAAG
TTCAAGCAGC AGAAATATGA GATCAATGTT CTCCGAAACA GGATCAACGA TAACCAGAAA
GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25054
Clone ID Related Accession (Same CDS sequence) NM_001276345 , XM_011509938
Accession Version NM_001276345.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79855.1, BC002653.2, L40162.1 and AA865519.1. Transcript Variant: This variant (5) encodes the longest isoform (5). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 12093807, 9689598 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG AGGCAGACTG AGCGGGAAAA GAAGAAGAAG
ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC ATTGACCACC TGAATGAAGA TCAGCTGAGG
GAGAAGGCCA AGGAGCTGTG GCAGAGCATC TATAACTTGG AGGCAGAGAA GTTCGACCTG
CAGGAGAAGT TCAAGCAGCA GAAATATGAG ATCAATGTTC TCCGAAACAG GATCAACGAT
AACCAGAAAG TCTCCAAGAC CCGCGGGAAG GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001263274.1
CDS93..989
Misc Feature(1)75..77(+)
Misc Feature(2)96..98(+)
Misc Feature(3)96..98(+)
Misc Feature(4)399..791(+)
Misc Feature(5)702..704(+)
Misc Feature(6)714..716(+)
Misc Feature(7)729..731(+)
Misc Feature(8)972..974(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..189
Gene:TNNT2
Gene Synonym:
Exon (6)190..255
Gene:TNNT2
Gene Synonym:
Exon (7)256..291
Gene:TNNT2
Gene Synonym:
Exon (8)292..325
Gene:TNNT2
Gene Synonym:
Exon (9)326..386
Gene:TNNT2
Gene Synonym:
Exon (10)387..503
Gene:TNNT2
Gene Synonym:
Exon (11)504..581
Gene:TNNT2
Gene Synonym:
Exon (12)582..692
Gene:TNNT2
Gene Synonym:
Exon (13)693..701
Gene:TNNT2
Gene Synonym:
Exon (14)702..811
Gene:TNNT2
Gene Synonym:
Exon (15)812..902
Gene:TNNT2
Gene Synonym:
Exon (16)903..943
Gene:TNNT2
Gene Synonym:
Exon (17)944..1191
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001276345
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001276345

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG AGGCAGACTG AGCGGGAAAA GAAGAAGAAG
ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC ATTGACCACC TGAATGAAGA TCAGCTGAGG
GAGAAGGCCA AGGAGCTGTG GCAGAGCATC TATAACTTGG AGGCAGAGAA GTTCGACCTG
CAGGAGAAGT TCAAGCAGCA GAAATATGAG ATCAATGTTC TCCGAAACAG GATCAACGAT
AACCAGAAAG TCTCCAAGAC CCGCGGGAAG GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24894
Clone ID Related Accession (Same CDS sequence) NM_001001432
Accession Version NM_001001432.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 849bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, X79861.1, BC002653.2 and AA865519.1. On Feb 5, 2013 this sequence version replaced gi:48255882. Transcript Variant: This variant (4) lacks multiple in-frame exons, compared to variant 5. It encodes a shorter isoform (4) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGGCCCA GACAGAGCGG AAAAGTGGGA AGAGGCAGAC TGAGCGGGAA
AAGAAGAAGA AGATTCTGGC TGAGAGGAGG AAGGTGCTGG CCATTGACCA CCTGAATGAA
GATCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAGCA TCTATAACTT GGAGGCAGAG
AAGTTCGACC TGCAGGAGAA GTTCAAGCAG CAGAAATATG AGATCAATGT TCTCCGAAAC
AGGATCAACG ATAACCAGAA AGTCTCCAAG ACCCGCGGGA AGGCTAAAGT CACCGGGCGC
TGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001001432.1
CDS93..941
Misc Feature(1)75..77(+)
Misc Feature(2)354..743(+)
Misc Feature(3)642..>884(+)
Misc Feature(4)654..656(+)
Misc Feature(5)666..668(+)
Misc Feature(6)681..683(+)
Misc Feature(7)924..926(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..210
Gene:TNNT2
Gene Synonym:
Exon (5)211..246
Gene:TNNT2
Gene Synonym:
Exon (6)247..280
Gene:TNNT2
Gene Synonym:
Exon (7)281..341
Gene:TNNT2
Gene Synonym:
Exon (8)342..458
Gene:TNNT2
Gene Synonym:
Exon (9)459..536
Gene:TNNT2
Gene Synonym:
Exon (10)537..647
Gene:TNNT2
Gene Synonym:
Exon (11)648..653
Gene:TNNT2
Gene Synonym:
Exon (12)654..763
Gene:TNNT2
Gene Synonym:
Exon (13)764..854
Gene:TNNT2
Gene Synonym:
Exon (14)855..895
Gene:TNNT2
Gene Synonym:
Exon (15)896..1143
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001001432
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001432

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGGCCCA GACAGAGCGG AAAAGTGGGA AGAGGCAGAC TGAGCGGGAA
AAGAAGAAGA AGATTCTGGC TGAGAGGAGG AAGGTGCTGG CCATTGACCA CCTGAATGAA
GATCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAGCA TCTATAACTT GGAGGCAGAG
AAGTTCGACC TGCAGGAGAA GTTCAAGCAG CAGAAATATG AGATCAATGT TCTCCGAAAC
AGGATCAACG ATAACCAGAA AGTCTCCAAG ACCCGCGGGA AGGCTAAAGT CACCGGGCGC
TGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24695
Clone ID Related Accession (Same CDS sequence) NM_001001430 , NM_001276347 , XM_006711508
Accession Version NM_001001430.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, BC002653.2, L40162.1 and AA865519.1. On Feb 5, 2013 this sequence version replaced gi:48255878. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 5. It encodes a shorter isoform (2) compared to isoform 5. Variants 2 and 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L40162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001001430.1
CDS93..959
Misc Feature(1)75..77(+)
Misc Feature(2)369..761(+)
Misc Feature(3)660..>902(+)
Misc Feature(4)672..674(+)
Misc Feature(5)684..686(+)
Misc Feature(6)699..701(+)
Misc Feature(7)942..944(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..225
Gene:TNNT2
Gene Synonym:
Exon (6)226..261
Gene:TNNT2
Gene Synonym:
Exon (7)262..295
Gene:TNNT2
Gene Synonym:
Exon (8)296..356
Gene:TNNT2
Gene Synonym:
Exon (9)357..473
Gene:TNNT2
Gene Synonym:
Exon (10)474..551
Gene:TNNT2
Gene Synonym:
Exon (11)552..662
Gene:TNNT2
Gene Synonym:
Exon (12)663..671
Gene:TNNT2
Gene Synonym:
Exon (13)672..781
Gene:TNNT2
Gene Synonym:
Exon (14)782..872
Gene:TNNT2
Gene Synonym:
Exon (15)873..913
Gene:TNNT2
Gene Synonym:
Exon (16)914..1161
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001001430
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001430

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24914
Clone ID Related Accession (Same CDS sequence) NM_001001431
Accession Version NM_001001431.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 858bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 04-AUG-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ709280.1, BC002653.2 and AA865519.1. On or before Aug 31, 2013 this sequence version replaced gi:530365340, gi:48255880. Transcript Variant: This variant (3) lacks multiple in-frame exons, compared to variant 5. It encodes a shorter isoform (3) compared to isoform 5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002653.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2155550, SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG ACAGAGCGGA AAAGTGGGAA GAGGCAGACT
GAGCGGGAAA AGAAGAAGAA GATTCTGGCT GAGAGGAGGA AGGTGCTGGC CATTGACCAC
CTGAATGAAG ATCAGCTGAG GGAGAAGGCC AAGGAGCTGT GGCAGAGCAT CTATAACTTG
GAGGCAGAGA AGTTCGACCT GCAGGAGAAG TTCAAGCAGC AGAAATATGA GATCAATGTT
CTCCGAAACA GGATCAACGA TAACCAGAAA GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC
ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001001431.1
CDS93..950
Misc Feature(1)75..77(+)
Misc Feature(2)369..752(+)
Misc Feature(3)663..665(+)
Misc Feature(4)675..677(+)
Misc Feature(5)690..692(+)
Misc Feature(6)933..935(+)
Exon (1)1..78
Gene:TNNT2
Gene Synonym:
Exon (2)79..133
Gene:TNNT2
Gene Synonym:
Exon (3)134..144
Gene:TNNT2
Gene Synonym:
Exon (4)145..159
Gene:TNNT2
Gene Synonym:
Exon (5)160..225
Gene:TNNT2
Gene Synonym:
Exon (6)226..261
Gene:TNNT2
Gene Synonym:
Exon (7)262..295
Gene:TNNT2
Gene Synonym:
Exon (8)296..356
Gene:TNNT2
Gene Synonym:
Exon (9)357..473
Gene:TNNT2
Gene Synonym:
Exon (10)474..551
Gene:TNNT2
Gene Synonym:
Exon (11)552..662
Gene:TNNT2
Gene Synonym:
Exon (12)663..772
Gene:TNNT2
Gene Synonym:
Exon (13)773..863
Gene:TNNT2
Gene Synonym:
Exon (14)864..904
Gene:TNNT2
Gene Synonym:
Exon (15)905..1152
Gene:TNNT2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001001431
Organism Homo sapiens (human)
Definition Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001001431

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG ACAGAGCGGA AAAGTGGGAA GAGGCAGACT
GAGCGGGAAA AGAAGAAGAA GATTCTGGCT GAGAGGAGGA AGGTGCTGGC CATTGACCAC
CTGAATGAAG ATCAGCTGAG GGAGAAGGCC AAGGAGCTGT GGCAGAGCAT CTATAACTTG
GAGGCAGAGA AGTTCGACCT GCAGGAGAAG TTCAAGCAGC AGAAATATGA GATCAATGTT
CTCCGAAACA GGATCAACGA TAACCAGAAA GTCTCCAAGA CCCGCGGGAA GGCTAAAGTC
ACCGGGCGCT GGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25054
Clone ID Related Accession (Same CDS sequence) NM_001276345 , XM_011509938
Accession Version XM_011509938.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 897bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG AGGCAGACTG AGCGGGAAAA GAAGAAGAAG
ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC ATTGACCACC TGAATGAAGA TCAGCTGAGG
GAGAAGGCCA AGGAGCTGTG GCAGAGCATC TATAACTTGG AGGCAGAGAA GTTCGACCTG
CAGGAGAAGT TCAAGCAGCA GAAATATGAG ATCAATGTTC TCCGAAACAG GATCAACGAT
AACCAGAAAG TCTCCAAGAC CCGCGGGAAG GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508240.1
CDS131..1027
Misc Feature(1)437..829(+)
Translation

Target ORF information:

RefSeq Version XM_011509938
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509938

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG AGGCAGACTG AGCGGGAAAA GAAGAAGAAG
ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC ATTGACCACC TGAATGAAGA TCAGCTGAGG
GAGAAGGCCA AGGAGCTGTG GCAGAGCATC TATAACTTGG AGGCAGAGAA GTTCGACCTG
CAGGAGAAGT TCAAGCAGCA GAAATATGAG ATCAATGTTC TCCGAAACAG GATCAACGAT
AACCAGAAAG TCTCCAAGAC CCGCGGGAAG GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61071
Clone ID Related Accession (Same CDS sequence) XM_011509939
Accession Version XM_011509939.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGACATCCAC
CGGAAGCGCA TGGAGAAGGA CCTGAATGAG TTGCAGGCGC TGATCGAGGC TCACTTTGAG
AACAGGAAGA AAGAGGAGGA GGAGCTCGTT TCTCTCAAAG ACAGGATCGA GAGACGTCGG
GCAGAGCGGG CCGAGCAGCA GCGCATCCGG AATGAGCGGG AGAAGGAGCG GCAGAACCGC
CTGGCTGAAG AGAGGGCTCG ACGAGAGGAG GAGGAGAACA GGAGGAAGGC TGAGGATGAG
GCCCGGAAGA AGAAGGCTTT GTCCAACATG ATGCATTTTG GGGGTTACAT CCAGAAGCAG
GCCCAGACAG AGCGGAAAAG TGGGAAGAGG CAGACTGAGC GGGAAAAGAA GAAGAAGATT
CTGGCTGAGA GGAGGAAGGT GCTGGCCATT GACCACCTGA ATGAAGATCA GCTGAGGGAG
AAGGCCAAGG AGCTGTGGCA GAGCATCTAT AACTTGGAGG CAGAGAAGTT CGACCTGCAG
GAGAAGTTCA AGCAGCAGAA ATATGAGATC AATGTTCTCC GAAACAGGAT CAACGATAAC
CAGAAAGTCT CCAAGACCCG CGGGAAGGCT AAAGTCACCG GGCGCTGGAA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508241.1
CDS140..1033
Misc Feature(1)443..835(+)
Misc Feature(2)734..>976(+)
Translation

Target ORF information:

RefSeq Version XM_011509939
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509939

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGACATCCAC
CGGAAGCGCA TGGAGAAGGA CCTGAATGAG TTGCAGGCGC TGATCGAGGC TCACTTTGAG
AACAGGAAGA AAGAGGAGGA GGAGCTCGTT TCTCTCAAAG ACAGGATCGA GAGACGTCGG
GCAGAGCGGG CCGAGCAGCA GCGCATCCGG AATGAGCGGG AGAAGGAGCG GCAGAACCGC
CTGGCTGAAG AGAGGGCTCG ACGAGAGGAG GAGGAGAACA GGAGGAAGGC TGAGGATGAG
GCCCGGAAGA AGAAGGCTTT GTCCAACATG ATGCATTTTG GGGGTTACAT CCAGAAGCAG
GCCCAGACAG AGCGGAAAAG TGGGAAGAGG CAGACTGAGC GGGAAAAGAA GAAGAAGATT
CTGGCTGAGA GGAGGAAGGT GCTGGCCATT GACCACCTGA ATGAAGATCA GCTGAGGGAG
AAGGCCAAGG AGCTGTGGCA GAGCATCTAT AACTTGGAGG CAGAGAAGTT CGACCTGCAG
GAGAAGTTCA AGCAGCAGAA ATATGAGATC AATGTTCTCC GAAACAGGAT CAACGATAAC
CAGAAAGTCT CCAAGACCCG CGGGAAGGCT AAAGTCACCG GGCGCTGGAA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61072
Clone ID Related Accession (Same CDS sequence) XM_011509940
Accession Version XM_011509940.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 894bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
GCCCAGACAG AGCGGAAAAG TGGGAAGAGG CAGACTGAGC GGGAAAAGAA GAAGAAGATT
CTGGCTGAGA GGAGGAAGGT GCTGGCCATT GACCACCTGA ATGAAGATCA GCTGAGGGAG
AAGGCCAAGG AGCTGTGGCA GAGCATCTAT AACTTGGAGG CAGAGAAGTT CGACCTGCAG
GAGAAGTTCA AGCAGCAGAA ATATGAGATC AATGTTCTCC GAAACAGGAT CAACGATAAC
CAGAAAGTCT CCAAGACCCG CGGGAAGGCT AAAGTCACCG GGCGCTGGAA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508242.1
CDS143..1036
Misc Feature(1)449..838(+)
Misc Feature(2)737..>979(+)
Translation

Target ORF information:

RefSeq Version XM_011509940
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509940

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTG AAGAGAGGGC TCGACGAGAG GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT
GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC ATGATGCATT TTGGGGGTTA CATCCAGAAG
GCCCAGACAG AGCGGAAAAG TGGGAAGAGG CAGACTGAGC GGGAAAAGAA GAAGAAGATT
CTGGCTGAGA GGAGGAAGGT GCTGGCCATT GACCACCTGA ATGAAGATCA GCTGAGGGAG
AAGGCCAAGG AGCTGTGGCA GAGCATCTAT AACTTGGAGG CAGAGAAGTT CGACCTGCAG
GAGAAGTTCA AGCAGCAGAA ATATGAGATC AATGTTCTCC GAAACAGGAT CAACGATAAC
CAGAAAGTCT CCAAGACCCG CGGGAAGGCT AAAGTCACCG GGCGCTGGAA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61073
Clone ID Related Accession (Same CDS sequence) XM_011509941
Accession Version XM_011509941.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 891bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGACATCCAC
CGGAAGCGCA TGGAGAAGGA CCTGAATGAG TTGCAGGCGC TGATCGAGGC TCACTTTGAG
AACAGGAAGA AAGAGGAGGA GGAGCTCGTT TCTCTCAAAG ACAGGATCGA GAGACGTCGG
GCAGAGCGGG CCGAGCAGCA GCGCATCCGG AATGAGCGGG AGAAGGAGCG GCAGAACCGC
CTGGCTGAAG AGAGGGCTCG ACGAGAGGAG GAGGAGAACA GGAGGAAGGC TGAGGATGAG
GCCCGGAAGA AGAAGGCTTT GTCCAACATG ATGCATTTTG GGGGTTACAT CCAGAAGGCC
CAGACAGAGC GGAAAAGTGG GAAGAGGCAG ACTGAGCGGG AAAAGAAGAA GAAGATTCTG
GCTGAGAGGA GGAAGGTGCT GGCCATTGAC CACCTGAATG AAGATCAGCT GAGGGAGAAG
GCCAAGGAGC TGTGGCAGAG CATCTATAAC TTGGAGGCAG AGAAGTTCGA CCTGCAGGAG
AAGTTCAAGC AGCAGAAATA TGAGATCAAT GTTCTCCGAA ACAGGATCAA CGATAACCAG
AAAGTCTCCA AGACCCGCGG GAAGGCTAAA GTCACCGGGC GCTGGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508243.1
CDS145..1035
Misc Feature(1)448..837(+)
Misc Feature(2)736..>978(+)
Translation

Target ORF information:

RefSeq Version XM_011509941
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509941

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGATGAAGA AGAAGAGGAA
GCAAAGGAGG CTGAAGATGG CCCAATGGAG GAGTCCAAAC CAAAGCCCAG GTCGTTCATG
CCCAACTTGG TGCCTCCCAA GATCCCCGAT GGAGAGAGAG TGGACTTTGA TGACATCCAC
CGGAAGCGCA TGGAGAAGGA CCTGAATGAG TTGCAGGCGC TGATCGAGGC TCACTTTGAG
AACAGGAAGA AAGAGGAGGA GGAGCTCGTT TCTCTCAAAG ACAGGATCGA GAGACGTCGG
GCAGAGCGGG CCGAGCAGCA GCGCATCCGG AATGAGCGGG AGAAGGAGCG GCAGAACCGC
CTGGCTGAAG AGAGGGCTCG ACGAGAGGAG GAGGAGAACA GGAGGAAGGC TGAGGATGAG
GCCCGGAAGA AGAAGGCTTT GTCCAACATG ATGCATTTTG GGGGTTACAT CCAGAAGGCC
CAGACAGAGC GGAAAAGTGG GAAGAGGCAG ACTGAGCGGG AAAAGAAGAA GAAGATTCTG
GCTGAGAGGA GGAAGGTGCT GGCCATTGAC CACCTGAATG AAGATCAGCT GAGGGAGAAG
GCCAAGGAGC TGTGGCAGAG CATCTATAAC TTGGAGGCAG AGAAGTTCGA CCTGCAGGAG
AAGTTCAAGC AGCAGAAATA TGAGATCAAT GTTCTCCGAA ACAGGATCAA CGATAACCAG
AAAGTCTCCA AGACCCGCGG GAAGGCTAAA GTCACCGGGC GCTGGAAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24695
Clone ID Related Accession (Same CDS sequence) NM_001001430 , NM_001276347 , XM_006711508
Accession Version XM_006711508.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578801422. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006711571.1
CDS129..995
Misc Feature(1)405..797(+)
Misc Feature(2)696..>938(+)
Translation

Target ORF information:

RefSeq Version XM_006711508
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006711508

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGAAGATGA AGAAGAAGAG GAAGCAAAGG AGGCTGAAGA TGGCCCAATG
GAGGAGTCCA AACCAAAGCC CAGGTCGTTC ATGCCCAACT TGGTGCCTCC CAAGATCCCC
GATGGAGAGA GAGTGGACTT TGATGACATC CACCGGAAGC GCATGGAGAA GGACCTGAAT
GAGTTGCAGG CGCTGATCGA GGCTCACTTT GAGAACAGGA AGAAAGAGGA GGAGGAGCTC
GTTTCTCTCA AAGACAGGAT CGAGAGACGT CGGGCAGAGC GGGCCGAGCA GCAGCGCATC
CGGAATGAGC GGGAGAAGGA GCGGCAGAAC CGCCTGGCTG AAGAGAGGGC TCGACGAGAG
GAGGAGGAGA ACAGGAGGAA GGCTGAGGAT GAGGCCCGGA AGAAGAAGGC TTTGTCCAAC
ATGATGCATT TTGGGGGTTA CATCCAGAAG CAGGCCCAGA CAGAGCGGAA AAGTGGGAAG
AGGCAGACTG AGCGGGAAAA GAAGAAGAAG ATTCTGGCTG AGAGGAGGAA GGTGCTGGCC
ATTGACCACC TGAATGAAGA TCAGCTGAGG GAGAAGGCCA AGGAGCTGTG GCAGAGCATC
TATAACTTGG AGGCAGAGAA GTTCGACCTG CAGGAGAAGT TCAAGCAGCA GAAATATGAG
ATCAATGTTC TCCGAAACAG GATCAACGAT AACCAGAAAG TCTCCAAGAC CCGCGGGAAG
GCTAAAGTCA CCGGGCGCTG GAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34665
Clone ID Related Accession (Same CDS sequence) XM_006711509
Accession Version XM_006711509.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 864bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578801424. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGATGAAGA AGAAGAGGAA GCAAAGGAGG CTGAAGATGG CCCAATGGAG
GAGTCCAAAC CAAAGCCCAG GTCGTTCATG CCCAACTTGG TGCCTCCCAA GATCCCCGAT
GGAGAGAGAG TGGACTTTGA TGACATCCAC CGGAAGCGCA TGGAGAAGGA CCTGAATGAG
TTGCAGGCGC TGATCGAGGC TCACTTTGAG AACAGGAAGA AAGAGGAGGA GGAGCTCGTT
TCTCTCAAAG ACAGGATCGA GAGACGTCGG GCAGAGCGGG CCGAGCAGCA GCGCATCCGG
AATGAGCGGG AGAAGGAGCG GCAGAACCGC CTGGCTGAAG AGAGGGCTCG ACGAGAGGAG
GAGGAGAACA GGAGGAAGGC TGAGGATGAG GCCCGGAAGA AGAAGGCTTT GTCCAACATG
ATGCATTTTG GGGGTTACAT CCAGAAGCAG GCCCAGACAG AGCGGAAAAG TGGGAAGAGG
CAGACTGAGC GGGAAAAGAA GAAGAAGATT CTGGCTGAGA GGAGGAAGGT GCTGGCCATT
GACCACCTGA ATGAAGATCA GCTGAGGGAG AAGGCCAAGG AGCTGTGGCA GAGCATCTAT
AACTTGGAGG CAGAGAAGTT CGACCTGCAG GAGAAGTTCA AGCAGCAGAA ATATGAGATC
AATGTTCTCC GAAACAGGAT CAACGATAAC CAGAAAGTCT CCAAGACCCG CGGGAAGGCT
AAAGTCACCG GGCGCTGGAA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006711572.1
CDS139..1002
Misc Feature(1)412..804(+)
Misc Feature(2)703..>945(+)
Translation

Target ORF information:

RefSeq Version XM_006711509
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006711509

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAGC AGGAGGAGGC AGCGGAAGAG GATGCTGAAG CAGAGGCTGA GACCGAGGAG
ACCAGGGCAG AAGATGAAGA AGAAGAGGAA GCAAAGGAGG CTGAAGATGG CCCAATGGAG
GAGTCCAAAC CAAAGCCCAG GTCGTTCATG CCCAACTTGG TGCCTCCCAA GATCCCCGAT
GGAGAGAGAG TGGACTTTGA TGACATCCAC CGGAAGCGCA TGGAGAAGGA CCTGAATGAG
TTGCAGGCGC TGATCGAGGC TCACTTTGAG AACAGGAAGA AAGAGGAGGA GGAGCTCGTT
TCTCTCAAAG ACAGGATCGA GAGACGTCGG GCAGAGCGGG CCGAGCAGCA GCGCATCCGG
AATGAGCGGG AGAAGGAGCG GCAGAACCGC CTGGCTGAAG AGAGGGCTCG ACGAGAGGAG
GAGGAGAACA GGAGGAAGGC TGAGGATGAG GCCCGGAAGA AGAAGGCTTT GTCCAACATG
ATGCATTTTG GGGGTTACAT CCAGAAGCAG GCCCAGACAG AGCGGAAAAG TGGGAAGAGG
CAGACTGAGC GGGAAAAGAA GAAGAAGATT CTGGCTGAGA GGAGGAAGGT GCTGGCCATT
GACCACCTGA ATGAAGATCA GCTGAGGGAG AAGGCCAAGG AGCTGTGGCA GAGCATCTAT
AACTTGGAGG CAGAGAAGTT CGACCTGCAG GAGAAGTTCA AGCAGCAGAA ATATGAGATC
AATGTTCTCC GAAACAGGAT CAACGATAAC CAGAAAGTCT CCAAGACCCG CGGGAAGGCT
AAAGTCACCG GGCGCTGGAA ATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34668
Clone ID Related Accession (Same CDS sequence) XM_011509942 , XM_011509943
Accession Version XM_011509942.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 852bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGCAGGC CCAGACAGAG CGGAAAAGTG GGAAGAGGCA GACTGAGCGG
GAAAAGAAGA AGAAGATTCT GGCTGAGAGG AGGAAGGTGC TGGCCATTGA CCACCTGAAT
GAAGATCAGC TGAGGGAGAA GGCCAAGGAG CTGTGGCAGA GCATCTATAA CTTGGAGGCA
GAGAAGTTCG ACCTGCAGGA GAAGTTCAAG CAGCAGAAAT ATGAGATCAA TGTTCTCCGA
AACAGGATCA ACGATAACCA GAAAGTCTCC AAGACCCGCG GGAAGGCTAA AGTCACCGGG
CGCTGGAAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508244.1
CDS138..989
Misc Feature(1)399..791(+)
Misc Feature(2)690..>932(+)
Translation

Target ORF information:

RefSeq Version XM_011509942
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509942

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGCAGGC CCAGACAGAG CGGAAAAGTG GGAAGAGGCA GACTGAGCGG
GAAAAGAAGA AGAAGATTCT GGCTGAGAGG AGGAAGGTGC TGGCCATTGA CCACCTGAAT
GAAGATCAGC TGAGGGAGAA GGCCAAGGAG CTGTGGCAGA GCATCTATAA CTTGGAGGCA
GAGAAGTTCG ACCTGCAGGA GAAGTTCAAG CAGCAGAAAT ATGAGATCAA TGTTCTCCGA
AACAGGATCA ACGATAACCA GAAAGTCTCC AAGACCCGCG GGAAGGCTAA AGTCACCGGG
CGCTGGAAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34668
Clone ID Related Accession (Same CDS sequence) XM_011509942 , XM_011509943
Accession Version XM_011509943.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 852bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGCAGGC CCAGACAGAG CGGAAAAGTG GGAAGAGGCA GACTGAGCGG
GAAAAGAAGA AGAAGATTCT GGCTGAGAGG AGGAAGGTGC TGGCCATTGA CCACCTGAAT
GAAGATCAGC TGAGGGAGAA GGCCAAGGAG CTGTGGCAGA GCATCTATAA CTTGGAGGCA
GAGAAGTTCG ACCTGCAGGA GAAGTTCAAG CAGCAGAAAT ATGAGATCAA TGTTCTCCGA
AACAGGATCA ACGATAACCA GAAAGTCTCC AAGACCCGCG GGAAGGCTAA AGTCACCGGG
CGCTGGAAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508245.1
CDS129..980
Misc Feature(1)390..782(+)
Misc Feature(2)681..>923(+)
Translation

Target ORF information:

RefSeq Version XM_011509943
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509943

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAA
GATGAAGAAG AAGAGGAAGC AAAGGAGGCT GAAGATGGCC CAATGGAGGA GTCCAAACCA
AAGCCCAGGT CGTTCATGCC CAACTTGGTG CCTCCCAAGA TCCCCGATGG AGAGAGAGTG
GACTTTGATG ACATCCACCG GAAGCGCATG GAGAAGGACC TGAATGAGTT GCAGGCGCTG
ATCGAGGCTC ACTTTGAGAA CAGGAAGAAA GAGGAGGAGG AGCTCGTTTC TCTCAAAGAC
AGGATCGAGA GACGTCGGGC AGAGCGGGCC GAGCAGCAGC GCATCCGGAA TGAGCGGGAG
AAGGAGCGGC AGAACCGCCT GGCTGAAGAG AGGGCTCGAC GAGAGGAGGA GGAGAACAGG
AGGAAGGCTG AGGATGAGGC CCGGAAGAAG AAGGCTTTGT CCAACATGAT GCATTTTGGG
GGTTACATCC AGAAGCAGGC CCAGACAGAG CGGAAAAGTG GGAAGAGGCA GACTGAGCGG
GAAAAGAAGA AGAAGATTCT GGCTGAGAGG AGGAAGGTGC TGGCCATTGA CCACCTGAAT
GAAGATCAGC TGAGGGAGAA GGCCAAGGAG CTGTGGCAGA GCATCTATAA CTTGGAGGCA
GAGAAGTTCG ACCTGCAGGA GAAGTTCAAG CAGCAGAAAT ATGAGATCAA TGTTCTCCGA
AACAGGATCA ACGATAACCA GAAAGTCTCC AAGACCCGCG GGAAGGCTAA AGTCACCGGG
CGCTGGAAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu34669
Clone ID Related Accession (Same CDS sequence) XM_011509944
Accession Version XM_011509944.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 849bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAT
GAAGAAGAAG AGGAAGCAAA GGAGGCTGAA GATGGCCCAA TGGAGGAGTC CAAACCAAAG
CCCAGGTCGT TCATGCCCAA CTTGGTGCCT CCCAAGATCC CCGATGGAGA GAGAGTGGAC
TTTGATGACA TCCACCGGAA GCGCATGGAG AAGGACCTGA ATGAGTTGCA GGCGCTGATC
GAGGCTCACT TTGAGAACAG GAAGAAAGAG GAGGAGGAGC TCGTTTCTCT CAAAGACAGG
ATCGAGAGAC GTCGGGCAGA GCGGGCCGAG CAGCAGCGCA TCCGGAATGA GCGGGAGAAG
GAGCGGCAGA ACCGCCTGGC TGAAGAGAGG GCTCGACGAG AGGAGGAGGA GAACAGGAGG
AAGGCTGAGG ATGAGGCCCG GAAGAAGAAG GCTTTGTCCA ACATGATGCA TTTTGGGGGT
TACATCCAGA AGCAGGCCCA GACAGAGCGG AAAAGTGGGA AGAGGCAGAC TGAGCGGGAA
AAGAAGAAGA AGATTCTGGC TGAGAGGAGG AAGGTGCTGG CCATTGACCA CCTGAATGAA
GATCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAGCA TCTATAACTT GGAGGCAGAG
AAGTTCGACC TGCAGGAGAA GTTCAAGCAG CAGAAATATG AGATCAATGT TCTCCGAAAC
AGGATCAACG ATAACCAGAA AGTCTCCAAG ACCCGCGGGA AGGCTAAAGT CACCGGGCGC
TGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508246.1
CDS139..987
Misc Feature(1)397..789(+)
Misc Feature(2)688..>930(+)
Translation

Target ORF information:

RefSeq Version XM_011509944
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509944

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAGCAGGAG 
GAGGCAGCGG AAGAGGATGC TGAAGCAGAG GCTGAGACCG AGGAGACCAG GGCAGAAGAT
GAAGAAGAAG AGGAAGCAAA GGAGGCTGAA GATGGCCCAA TGGAGGAGTC CAAACCAAAG
CCCAGGTCGT TCATGCCCAA CTTGGTGCCT CCCAAGATCC CCGATGGAGA GAGAGTGGAC
TTTGATGACA TCCACCGGAA GCGCATGGAG AAGGACCTGA ATGAGTTGCA GGCGCTGATC
GAGGCTCACT TTGAGAACAG GAAGAAAGAG GAGGAGGAGC TCGTTTCTCT CAAAGACAGG
ATCGAGAGAC GTCGGGCAGA GCGGGCCGAG CAGCAGCGCA TCCGGAATGA GCGGGAGAAG
GAGCGGCAGA ACCGCCTGGC TGAAGAGAGG GCTCGACGAG AGGAGGAGGA GAACAGGAGG
AAGGCTGAGG ATGAGGCCCG GAAGAAGAAG GCTTTGTCCA ACATGATGCA TTTTGGGGGT
TACATCCAGA AGCAGGCCCA GACAGAGCGG AAAAGTGGGA AGAGGCAGAC TGAGCGGGAA
AAGAAGAAGA AGATTCTGGC TGAGAGGAGG AAGGTGCTGG CCATTGACCA CCTGAATGAA
GATCAGCTGA GGGAGAAGGC CAAGGAGCTG TGGCAGAGCA TCTATAACTT GGAGGCAGAG
AAGTTCGACC TGCAGGAGAA GTTCAAGCAG CAGAAATATG AGATCAATGT TCTCCGAAAC
AGGATCAACG ATAACCAGAA AGTCTCCAAG ACCCGCGGGA AGGCTAAAGT CACCGGGCGC
TGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61074
Clone ID Related Accession (Same CDS sequence) XM_011509945
Accession Version XM_011509945.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 732bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTT GCAGACTTCG TGGGAACCGA AGGCCCCGTA GCTCTCTTCC TGTGCAGCTG
CTGAAGGCAT TTCCAGAGCA ACAGGTCTTC CAGGGGCACT CCCAGCTTAA GGGGATGACG
AGACCTCACA TTTGGGAATC AGGACCTCAT TCAATCACGC AGTCATTTCT CAGTCAGTGG
ACTCCTGTGG GCCAGCACTG TGCTGGGAGC TACCCTCTCA GAAAGCTCCT TGCTGAGCGG
AGAGAAAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508247.1
CDS158..889
Misc Feature(1)464..>646(+)
Translation

Target ORF information:

RefSeq Version XM_011509945
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509945

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGTCTGACA TAGAAGAGGT GGTGGAAGAG TACGAGGAGG AGGAGCAGGA AGAAGCAGCT 
GTTGAAGAAG AGGAGGACTG GAGAGAGGAC GAAGACGAGC AGGAGGAGGC AGCGGAAGAG
GATGCTGAAG CAGAGGCTGA GACCGAGGAG ACCAGGGCAG AAGAAGATGA AGAAGAAGAG
GAAGCAAAGG AGGCTGAAGA TGGCCCAATG GAGGAGTCCA AACCAAAGCC CAGGTCGTTC
ATGCCCAACT TGGTGCCTCC CAAGATCCCC GATGGAGAGA GAGTGGACTT TGATGACATC
CACCGGAAGC GCATGGAGAA GGACCTGAAT GAGTTGCAGG CGCTGATCGA GGCTCACTTT
GAGAACAGGA AGAAAGAGGA GGAGGAGCTC GTTTCTCTCA AAGACAGGAT CGAGAGACGT
CGGGCAGAGC GGGCCGAGCA GCAGCGCATC CGGAATGAGC GGGAGAAGGA GCGGCAGAAC
CGCCTGGCTT GCAGACTTCG TGGGAACCGA AGGCCCCGTA GCTCTCTTCC TGTGCAGCTG
CTGAAGGCAT TTCCAGAGCA ACAGGTCTTC CAGGGGCACT CCCAGCTTAA GGGGATGACG
AGACCTCACA TTTGGGAATC AGGACCTCAT TCAATCACGC AGTCATTTCT CAGTCAGTGG
ACTCCTGTGG GCCAGCACTG TGCTGGGAGC TACCCTCTCA GAAAGCTCCT TGCTGAGCGG
AGAGAAAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61075
Clone ID Related Accession (Same CDS sequence) XM_011509946
Accession Version XM_011509946.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 690bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product troponin T, cardiac muscle isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_004487.20) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGAGT CCAAACCAAA GCCCAGGTCG TTCATGCCCA ACTTGGTGCC TCCCAAGATC 
CCCGATGGAG AGAGAGTGGA CTTTGATGAC ATCCACCGGA AGCGCATGGA GAAGGACCTG
AATGAGTTGC AGGCGCTGAT CGAGGCTCAC TTTGAGAACA GGAAGAAAGA GGAGGAGGAG
CTCGTTTCTC TCAAAGACAG GATCGAGAGA CGTCGGGCAG AGCGGGCCGA GCAGCAGCGC
ATCCGGAATG AGCGGGAGAA GGAGCGGCAG AACCGCCTGG CTGAAGAGAG GGCTCGACGA
GAGGAGGAGG AGAACAGGAG GAAGGCTGAG GATGAGGCCC GGAAGAAGAA GGCTTTGTCC
AACATGATGC ATTTTGGGGG TTACATCCAG AAGCAGGCCC AGACAGAGCG GAAAAGTGGG
AAGAGGCAGA CTGAGCGGGA AAAGAAGAAG AAGATTCTGG CTGAGAGGAG GAAGGTGCTG
GCCATTGACC ACCTGAATGA AGATCAGCTG AGGGAGAAGG CCAAGGAGCT GTGGCAGAGC
ATCTATAACT TGGAGGCAGA GAAGTTCGAC CTGCAGGAGA AGTTCAAGCA GCAGAAATAT
GAGATCAATG TTCTCCGAAA CAGGATCAAC GATAACCAGA AAGTCTCCAA GACCCGCGGG
AAGGCTAAAG TCACCGGGCG CTGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508248.1
CDS373..1062
Misc Feature(1)472..864(+)
Translation

Target ORF information:

RefSeq Version XM_011509946
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011509946

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGGAGT CCAAACCAAA GCCCAGGTCG TTCATGCCCA ACTTGGTGCC TCCCAAGATC 
CCCGATGGAG AGAGAGTGGA CTTTGATGAC ATCCACCGGA AGCGCATGGA GAAGGACCTG
AATGAGTTGC AGGCGCTGAT CGAGGCTCAC TTTGAGAACA GGAAGAAAGA GGAGGAGGAG
CTCGTTTCTC TCAAAGACAG GATCGAGAGA CGTCGGGCAG AGCGGGCCGA GCAGCAGCGC
ATCCGGAATG AGCGGGAGAA GGAGCGGCAG AACCGCCTGG CTGAAGAGAG GGCTCGACGA
GAGGAGGAGG AGAACAGGAG GAAGGCTGAG GATGAGGCCC GGAAGAAGAA GGCTTTGTCC
AACATGATGC ATTTTGGGGG TTACATCCAG AAGCAGGCCC AGACAGAGCG GAAAAGTGGG
AAGAGGCAGA CTGAGCGGGA AAAGAAGAAG AAGATTCTGG CTGAGAGGAG GAAGGTGCTG
GCCATTGACC ACCTGAATGA AGATCAGCTG AGGGAGAAGG CCAAGGAGCT GTGGCAGAGC
ATCTATAACT TGGAGGCAGA GAAGTTCGAC CTGCAGGAGA AGTTCAAGCA GCAGAAATAT
GAGATCAATG TTCTCCGAAA CAGGATCAAC GATAACCAGA AAGTCTCCAA GACCCGCGGG
AAGGCTAAAG TCACCGGGCG CTGGAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Soluble suppression of tumorigenicity 2 (sST2), but not galactin-3, adds to prognostication in patients with systemic AL amyloidosis independent of NT-proBNP and troponin T
Am. J. Hematol. 90 (6), 524-528 (2015)
Dispenzieri A, Gertz MA, Saenger A, Kumar SK, Lacy MQ, Buadi FK, Dingli D, Leung N, Zeldenrust S, Hayman SR, Kapoor P, Grogan M, Hwa L, Russell SJ, Go RS, Rajkumar SV, Kyle RA and Jaffe A.


book

NH2-terminal truncations of cardiac troponin I and cardiac troponin T produce distinct effects on contractility and calcium homeostasis in adult cardiomyocytes
Am. J. Physiol., Cell Physiol. 308 (5), C397-C404 (2015)
Wei H and Jin JP.


book

Markers of cardiac dysfunction in cognitive impairment and dementia
Medicine (Baltimore) 94 (1), E297 (2015)
Hilal S, Chai YL, Ikram MK, Elangovan S, Yeow TB, Xin X, Chong JY, Venketasubramanian N, Richards AM, Chong JP, Lai MK and Chen C.


book

Clinical phenotype and outcome of hypertrophic cardiomyopathy associated with thin-filament gene mutations
J. Am. Coll. Cardiol. 64 (24), 2589-2600 (2014)
Coppini R, Ho CY, Ashley E, Day S, Ferrantini C, Girolami F, Tomberli B, Bardi S, Torricelli F, Cecchi F, Mugelli A, Poggesi C, Tardiff J and Olivotto I.


book

Hypertrophic Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Cirino,A.L. and Ho,C.


book

Dilated Cardiomyopathy Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Hershberger,R.E. and Morales,A.


book

Identification of sites phosphorylated in bovine cardiac troponin I and troponin T by protein kinase C and comparative substrate activity of synthetic peptides containing the phosphorylation sites
J. Biol. Chem. 264 (34), 20778-20785 (1989)
Noland TA Jr, Raynor RL and Kuo JF.


book

Ca2+, pH and the regulation of cardiac myofilament force and ATPase activity
Mol. Cell. Biochem. 89 (2), 163-167 (1989)
Solaro RJ, el-Saleh SC and Kentish JC.


book

Developmentally induced, muscle-specific trans factors control the differential splicing of alternative and constitutive troponin T exons
Cell 49 (6), 793-803 (1987)
Breitbart,R.E. and Nadal-Ginard,B.


book

A novel mechanism of alternative RNA splicing for the developmentally regulated generation of troponin T isoforms from a single gene
Cell 38 (2), 409-421 (1984)
Medford,R.M., Nguyen,H.T., Destree,A.T., Summers,E. and Nadal-Ginard,B.


 
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