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TRPC6 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TRPC6
Entrez Gene ID 7225
Full Name transient receptor potential cation channel subfamily C member 6
Synonyms FSGS2,TRP6
General protein information
Preferred Names

transient receptor potential cation channel subfamily C member 6

Names

short transient receptor potential channel 6
TRP-6
focal segmental glomerulosclerosis 2
transient receptor protein 6

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q22.1

Summary The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq, Mar 2009].
Disorder MIM:

603652

Disorder Html: Glomerulosclerosis, focal segmental, 2, 603965 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_004621.5 NP_004612.2 short transient receptor potential channel 6
XM_011542968.2 XP_011541270.1 short transient receptor potential channel 6 isoform X1
XM_017018221.1 XP_016873710.1 short transient receptor potential channel 6 isoform X2
XM_017018222.1 XP_016873711.1 short transient receptor potential channel 6 isoform X3



Bos taurus (cattle) TRPC6 NP_001160044.1
Caenorhabditis elegans (roundworm) trp-1 NP_001040889.1
Xenopus tropicalis (tropical clawed frog) trpc6 XP_002935616.2
Macaca mulatta (rhesus monkey) TRPM2 XP_002808133.1
Canis lupus familiaris (dog) TRPC6 XP_546553.3
Danio rerio (zebrafish) trpc6a NP_001025453.1
Gallus gallus (chicken) TRPC6 XP_003640636.1
Danio rerio (zebrafish) LOC100333090 XP_002665445.2
Mus musculus (house mouse) Trpc6 NP_038866.2
Homo sapiens (human) TRPC6 NP_004612.2
Pan troglodytes (chimpanzee) TRPC6 XP_001150152.1
Rattus norvegicus (Norway rat) Trpc6 NP_446011.1


Related articles in PubMed

Membrane translocation of TRPC6 channels and endothelial migration are regulated by calmodulin and PI3 kinase activation.
Chaudhuri P, Rosenbaum MA, Sinharoy P, Damron DS, Birnbaumer L, Graham LM
Proceedings of the National Academy of Sciences of the United States of America113(8)2110-5(2016 Feb)

TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production.
Wang J, Lu R, Yang J, Li H, He Z, Jing N, Wang X, Wang Y
Nature communications68876(2015 Nov)

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.
Riazanski V, Gabdoulkhakova AG, Boynton LS, Eguchi RR, Deriy LV, Hogarth DK, Loaëc N, Oumata N, Galons H, Brown ME, Shevchenko P, Gallan AJ, Yoo SG, Naren AP, Villereal ML, Beacham DW, Bindokas VP, Birnbaumer L, Meijer L, Nelson DJ
Proceedings of the National Academy of Sciences of the United States of America112(47)E6486-95(2015 Nov)

TRPC6 gene variants and neuropsychiatric lupus.
Ramirez GA, Lanzani C, Bozzolo EP, Citterio L, Zagato L, Casamassima N, Canti V, Sabbadini MG, Rovere-Querini P, Manunta P, Manfredi AA
Journal of neuroimmunology28821-4(2015 Nov)

TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response.
Weber EW, Han F, Tauseef M, Birnbaumer L, Mehta D, Muller WA
The Journal of experimental medicine212(11)1883-99(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

n response to stretching (20%), ATP was released only from the foremost cells at the wound edge; it then diffused to the cells behind the wound edge and activated the P2Y receptors, which caused propagating Ca(2+) waves via TRPC6
Title: Hyperforin/HP-

SARAF modulates TRPC1, but not TRPC6, channel function in a STIM1-independent manner
Title: SARAF modulates TRPC1, but not TRPC6, channel function in a STIM1-independent manner.

Functional interaction of upregulated CaSR and upregulated TRPC6 in pulmonary artery smooth muscle cells from idiopathic pulmonary arterial hypertension patients may play an important role in the development and progression of sustained pulmonary vasoconstriction and pulmonary vascular remodeling.
Title: Pathogenic role of calcium-sensing receptors in the development and progression of pulmonary hypertension.

study demonstrated that the various mechanisms regulating MDR in HCC cells are calcium dependent through the TRPC6/calcium/STAT3 pathway. We propose that targeting TRPC6 in HCC may be a novel antineoplastic strategy, especially combined with chemotherapy
Title: Regulation of Multi-drug Resistance in hepatocellular carcinoma cells is TRPC6/Calcium Dependent.

potential implications of transient receptor potential (TRP) channels in the pathogenesis of intestinal fibrosis, since they are known to act as cellular stress sensors/transducers affecting intracellular Ca(2+) homeostasis/dynamics, and are involved in a broad spectrum of cell pathophysiology including inflammation and tissue remodeling.
Title: Significant contribution of TRPC6 channel-mediated Ca

The following TRPC6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPC6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23592
NM_004621.5
Latest version!
Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu92470 XM_017018222.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 10-12 $475.30
$679.00
OHu61147 XM_011542968.2
Latest version!
Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu92469 XM_017018221.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23592
Clone ID Related Accession (Same CDS sequence) NM_004621.5
Accession Version NM_004621.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-03
Organism Homo sapiens(Human)
Product short transient receptor potential channel 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ006276.1 and AK001960.1. This sequence is a reference standard in the RefSeqGene project. On Mar 15, 2008 this sequence version replaced NM_004621.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.174836.1, AJ006276.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGAGCCAGA GCCCGGCGTT CGGGCCCCGG AGGGGCAGTT CTCCCCGGGG CGCTGCCGGA 
GCCGCTGCGC GGCGCAACGA GAGCCAGGAC TATCTGCTCA TGGACTCGGA GCTGGGAGAA
GACGGCTGCC CGCAAGCCCC GCTGCCTTGC TACGGCTACT ACCCCTGCTT CCGGGGATCT
GACAACAGAC TGGCTCACCG GCGGCAGACA GTTCTCCGTG AGAAGGGGAG AAGGTTAGCT
AATCGAGGAC CAGCATACAT GTTTAGTGAT CGCTCCACAA GCCTATCTAT AGAGGAGGAA
CGCTTTTTGG ATGCAGCTGA ATATGGTAAC ATCCCAGTGG TGCGGAAGAT GTTAGAAGAA
TGCCACTCAC TCAACGTTAA CTGTGTGGAT TACATGGGCC AGAATGCCCT ACAGTTGGCA
GTGGCCAATG AGCATCTGGA AATTACAGAA CTTCTTCTCA AGAAAGAAAA CCTCTCTCGA
GTTGGGGATG CTTTGCTTCT AGCTATTAGT AAAGGTTATG TTCGGATTGT GGAAGCAATT
CTCAGTCATC CGGCTTTTGC TGAAGGCAAG AGGTTAGCAA CCAGCCCTAG CCAGTCTGAA
CTCCAGCAAG ATGATTTTTA TGCCTATGAT GAAGATGGGA CACGGTTCTC CCATGATGTG
ACTCCAATCA TTCTGGCTGC CCACTGCCAG GAATATGAAA TTGTGCATAC CCTCCTGCGG
AAGGGTGCTA GGATTGAACG GCCTCATGAT TATTTCTGCA AGTGCAATGA CTGCAACCAG
AAACAGAAGC ATGACTCGTT TAGCCACTCC AGATCTAGGA TTAATGCCTA TAAAGGCCTG
GCAAGTCCGG CTTACCTGTC ATTGTCTAGT GAAGATCCAG TCATGACGGC TTTAGAACTT
AGCAATGAAC TGGCAGTTCT GGCCAATATT GAGAAAGAGT TCAAGAATGA CTACAAAAAA
CTGTCAATGC AGTGCAAAGA CTTTGTTGTT GGACTCCTTG ATCTGTGCAG AAACACTGAA
GAAGTCGAGG CCATTCTGAA TGGGGATGTT GAAACGCTCC AGAGTGGTGA TCACGGTCGC
CCAAATCTCA GCCGTTTAAA ACTTGCCATT AAATATGAAG TAAAAAAATT TGTAGCTCAT
CCAAACTGCC AACAGCAACT TCTCTCCATT TGGTATGAGA ATCTTTCTGG TTTACGACAG
CAGACAATGG CGGTCAAGTT CCTTGTGGTC CTTGCTGTTG CCATTGGACT GCCCTTCCTG
GCTCTCATTT ACTGGTTTGC TCCATGCAGC AAGATGGGGA AGATAATGCG TGGACCATTC
ATGAAGTTTG TAGCACACGC AGCCTCCTTC ACCATTTTTC TGGGACTGCT AGTCATGAAT
GCAGCTGACA GATTTGAAGG CACAAAACTC CTTCCTAATG AAACCAGCAC AGATAATGCA
AAACAGCTGT TCAGGATGAA AACATCCTGC TTCTCATGGA TGGAGATGCT CATTATATCC
TGGGTAATAG GCATGATATG GGCTGAATGT AAAGAAATCT GGACTCAGGG CCCCAAGGAA
TATTTGTTTG AGTTGTGGAA CATGCTTGAT TTTGGTATGT TAGCAATTTT CGCAGCATCA
TTCATTGCGA GATTCATGGC ATTTTGGCAT GCTTCCAAAG CCCAGAGCAT CATTGACGCA
AATGATACTT TGAAGGACTT GACGAAAGTA ACATTGGGAG ACAATGTGAA ATACTACAAT
TTGGCCAGGA TAAAGTGGGA CCCCTCTGAT CCTCAAATAA TATCTGAAGG TCTTTATGCA
ATTGCTGTAG TTTTAAGTTT CTCTAGGATA GCTTATATTT TACCAGCAAA TGAAAGCTTT
GGACCTCTGC AGATATCACT TGGAAGAACA GTCAAAGACA TCTTCAAGTT CATGGTCATA
TTCATTATGG TGTTTGTGGC CTTTATGATT GGAATGTTCA ATCTCTACTC CTACTACATT
GGTGCAAAAC AAAATGAAGC CTTCACAACA GTTGAAGAGA GTTTTAAGAC ACTGTTCTGG
GCTATATTTG GACTTTCTGA AGTGAAATCA GTGGTCATCA ACTATAACCA CAAATTCATT
GAAAACATTG GTTACGTTCT TTATGGAGTC TATAATGTTA CGATGGTCAT TGTTTTGCTA
AATATGTTAA TTGCCATGAT CAACAGTTCA TTCCAGGAAA TTGAGGATGA CGCTGATGTG
GAGTGGAAAT TTGCAAGGGC CAAACTCTGG TTTTCCTACT TTGAGGAGGG CAGAACACTT
CCTGTACCCT TCAATCTGGT GCCGAGTCCA AAGTCCCTGT TTTATCTCTT ACTGAAGCTT
AAAAAATGGA TTTCTGAGCT GTTCCAGGGC CATAAAAAAG GTTTCCAGGA AGATGCAGAG
ATGAACAAGA TAAATGAAGA AAAGAAACTT GGAATTTTAG GAAGTCATGA AGACCTTTCA
AAATTATCAC TTGACAAAAA ACAGGTTGGG CACAATAAAC AACCAAGTAT AAGGAGCTCA
GAAGATTTCC ATCTAAATAG TTTCAATAAT CCTCCAAGAC AATATCAGAA AATAATGAAA
AGGCTCATTA AAAGATATGT ACTGCAGGCC CAGATAGATA AGGAGAGTGA TGAAGTGAAC
GAAGGGGAAC TGAAGGAAAT TAAGCAGGAC ATCTCAAGTC TCCGCTATGA ACTCCTTGAA
GAAAAATCTC AGAATACAGA AGACCTAGCA GAACTTATTA GAGAACTTGG AGAGAAATTA
TCCATGGAAC CAAATCAAGA GGAAACCAAT AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004612.2
CDS426..3221
Misc Feature(1)231..233
Misc Feature(2)669..3116
Misc Feature(3)714..803
Misc Feature(4)738..1142
Misc Feature(5)order(744..752,756..761,771..773,780..782,813..815,819..821,825..827,837..842,849..857,861..866,876..878,885..887,900..902,906..908,912..914,921..926,933..941,945..950,960..962,969..971)
Misc Feature(6)819..908
Misc Feature(7)819..902
Misc Feature(8)906..980
Misc Feature(9)912..992
Misc Feature(10)1059..1166
Misc Feature(11)1077..1166
Misc Feature(12)1182..1361
Misc Feature(13)1740..1802
Misc Feature(14)1887..1949
Misc Feature(15)1989..2051
Misc Feature(16)2202..2264
Misc Feature(17)2334..2396
Misc Feature(18)2544..2606
Misc Feature(19)2868..2870
Exon (1)1..595
Exon (2)596..1370
Exon (3)1371..1553
Exon (4)1554..1718
Exon (5)1719..1935
Exon (6)1936..2169
Exon (7)2170..2434
Exon (8)2435..2630
Exon (9)2631..2834
Exon (10)2835..2909
Exon (11)2910..2993
Exon (12)2994..3069
Exon (13)3070..4612
Translation

Target ORF information:

RefSeq Version NM_004621.5
Organism Homo sapiens(Human)
Definition Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004621.5

ORF Insert Sequence:

1
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181
241
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361
421
481
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601
661
721
781
841
901
961
1021
1081
1141
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1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGAGCCAGA GCCCGGCGTT CGGGCCCCGG AGGGGCAGTT CTCCCCGGGG CGCTGCCGGA 
GCCGCTGCGC GGCGCAACGA GAGCCAGGAC TATCTGCTCA TGGACTCGGA GCTGGGAGAA
GACGGCTGCC CGCAAGCCCC GCTGCCTTGC TACGGCTACT ACCCCTGCTT CCGGGGATCT
GACAACAGAC TGGCTCACCG GCGGCAGACA GTTCTCCGTG AGAAGGGGAG AAGGTTAGCT
AATCGAGGAC CAGCATACAT GTTTAGTGAT CGCTCCACAA GCCTATCTAT AGAGGAGGAA
CGCTTTTTGG ATGCAGCTGA ATATGGTAAC ATCCCAGTGG TGCGGAAGAT GTTAGAAGAA
TGCCACTCAC TCAACGTTAA CTGTGTGGAT TACATGGGCC AGAATGCCCT ACAGTTGGCA
GTGGCCAATG AGCATCTGGA AATTACAGAA CTTCTTCTCA AGAAAGAAAA CCTCTCTCGA
GTTGGGGATG CTTTGCTTCT AGCTATTAGT AAAGGTTATG TTCGGATTGT GGAAGCAATT
CTCAGTCATC CGGCTTTTGC TGAAGGCAAG AGGTTAGCAA CCAGCCCTAG CCAGTCTGAA
CTCCAGCAAG ATGATTTTTA TGCCTATGAT GAAGATGGGA CACGGTTCTC CCATGATGTG
ACTCCAATCA TTCTGGCTGC CCACTGCCAG GAATATGAAA TTGTGCATAC CCTCCTGCGG
AAGGGTGCTA GGATTGAACG GCCTCATGAT TATTTCTGCA AGTGCAATGA CTGCAACCAG
AAACAGAAGC ATGACTCGTT TAGCCACTCC AGATCTAGGA TTAATGCCTA TAAAGGCCTG
GCAAGTCCGG CTTACCTGTC ATTGTCTAGT GAAGATCCAG TCATGACGGC TTTAGAACTT
AGCAATGAAC TGGCAGTTCT GGCCAATATT GAGAAAGAGT TCAAGAATGA CTACAAAAAA
CTGTCAATGC AGTGCAAAGA CTTTGTTGTT GGACTCCTTG ATCTGTGCAG AAACACTGAA
GAAGTCGAGG CCATTCTGAA TGGGGATGTT GAAACGCTCC AGAGTGGTGA TCACGGTCGC
CCAAATCTCA GCCGTTTAAA ACTTGCCATT AAATATGAAG TAAAAAAATT TGTAGCTCAT
CCAAACTGCC AACAGCAACT TCTCTCCATT TGGTATGAGA ATCTTTCTGG TTTACGACAG
CAGACAATGG CGGTCAAGTT CCTTGTGGTC CTTGCTGTTG CCATTGGACT GCCCTTCCTG
GCTCTCATTT ACTGGTTTGC TCCATGCAGC AAGATGGGGA AGATAATGCG TGGACCATTC
ATGAAGTTTG TAGCACACGC AGCCTCCTTC ACCATTTTTC TGGGACTGCT AGTCATGAAT
GCAGCTGACA GATTTGAAGG CACAAAACTC CTTCCTAATG AAACCAGCAC AGATAATGCA
AAACAGCTGT TCAGGATGAA AACATCCTGC TTCTCATGGA TGGAGATGCT CATTATATCC
TGGGTAATAG GCATGATATG GGCTGAATGT AAAGAAATCT GGACTCAGGG CCCCAAGGAA
TATTTGTTTG AGTTGTGGAA CATGCTTGAT TTTGGTATGT TAGCAATTTT CGCAGCATCA
TTCATTGCGA GATTCATGGC ATTTTGGCAT GCTTCCAAAG CCCAGAGCAT CATTGACGCA
AATGATACTT TGAAGGACTT GACGAAAGTA ACATTGGGAG ACAATGTGAA ATACTACAAT
TTGGCCAGGA TAAAGTGGGA CCCCTCTGAT CCTCAAATAA TATCTGAAGG TCTTTATGCA
ATTGCTGTAG TTTTAAGTTT CTCTAGGATA GCTTATATTT TACCAGCAAA TGAAAGCTTT
GGACCTCTGC AGATATCACT TGGAAGAACA GTCAAAGACA TCTTCAAGTT CATGGTCATA
TTCATTATGG TGTTTGTGGC CTTTATGATT GGAATGTTCA ATCTCTACTC CTACTACATT
GGTGCAAAAC AAAATGAAGC CTTCACAACA GTTGAAGAGA GTTTTAAGAC ACTGTTCTGG
GCTATATTTG GACTTTCTGA AGTGAAATCA GTGGTCATCA ACTATAACCA CAAATTCATT
GAAAACATTG GTTACGTTCT TTATGGAGTC TATAATGTTA CGATGGTCAT TGTTTTGCTA
AATATGTTAA TTGCCATGAT CAACAGTTCA TTCCAGGAAA TTGAGGATGA CGCTGATGTG
GAGTGGAAAT TTGCAAGGGC CAAACTCTGG TTTTCCTACT TTGAGGAGGG CAGAACACTT
CCTGTACCCT TCAATCTGGT GCCGAGTCCA AAGTCCCTGT TTTATCTCTT ACTGAAGCTT
AAAAAATGGA TTTCTGAGCT GTTCCAGGGC CATAAAAAAG GTTTCCAGGA AGATGCAGAG
ATGAACAAGA TAAATGAAGA AAAGAAACTT GGAATTTTAG GAAGTCATGA AGACCTTTCA
AAATTATCAC TTGACAAAAA ACAGGTTGGG CACAATAAAC AACCAAGTAT AAGGAGCTCA
GAAGATTTCC ATCTAAATAG TTTCAATAAT CCTCCAAGAC AATATCAGAA AATAATGAAA
AGGCTCATTA AAAGATATGT ACTGCAGGCC CAGATAGATA AGGAGAGTGA TGAAGTGAAC
GAAGGGGAAC TGAAGGAAAT TAAGCAGGAC ATCTCAAGTC TCCGCTATGA ACTCCTTGAA
GAAAAATCTC AGAATACAGA AGACCTAGCA GAACTTATTA GAGAACTTGG AGAGAAATTA
TCCATGGAAC CAAATCAAGA GGAAACCAAT AGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92470
Clone ID Related Accession (Same CDS sequence) XM_017018222.1
Accession Version XM_017018222.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product short transient receptor potential channel 6 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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1621
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1981
2041
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2341
2401
2461
2521
ATGTTTAGTG ATCGCTCCAC AAGCCTATCT ATAGAGGAGG AACGCTTTTT GGATGCAGCT 
GAATATGGTA ACATCCCAGT GGTGCGGAAG ATGTTAGAAG AATGCCACTC ACTCAACGTT
AACTGTGTGG ATTACATGGG CCAGAATGCC CTACAGTTGG CAGTGGCCAA TGAGCATCTG
GAAATTACAG AACTTCTTCT CAAGAAAGAA AACCTCTCTC GAGTTGGGGA TGCTTTGCTT
CTAGCTATTA GTAAAGGTTA TGTTCGGATT GTGGAAGCAA TTCTCAGTCA TCCGGCTTTT
GCTGAAGGCA AGAGGTTAGC AACCAGCCCT AGCCAGTCTG AACTCCAGCA AGATGATTTT
TATGCCTATG ATGAAGATGG GACACGGTTC TCCCATGATG TGACTCCAAT CATTCTGGCT
GCCCACTGCC AGGAATATGA AATTGTGCAT ACCCTCCTGC GGAAGGGTGC TAGGATTGAA
CGGCCTCATG ATTATTTCTG CAAGTGCAAT GACTGCAACC AGAAACAGAA GCATGACTCG
TTTAGCCACT CCAGATCTAG GATTAATGCC TATAAAGGCC TGGCAAGTCC GGCTTACCTG
TCATTGTCTA GTGAAGATCC AGTCATGACG GCTTTAGAAC TTAGCAATGA ACTGGCAGTT
CTGGCCAATA TTGAGAAAGA GTTCAAGAAT GACTACAAAA AACTGTCAAT GCAGTGCAAA
GACTTTGTTG TTGGACTCCT TGATCTGTGC AGAAACACTG AAGAAGTCGA GGCCATTCTG
AATGGGGATG TTGAAACGCT CCAGAGTGGT GATCACGGTC GCCCAAATCT CAGCCGTTTA
AAACTTGCCA TTAAATATGA AGTAAAAAAA TTTGTAGCTC ATCCAAACTG CCAACAGCAA
CTTCTCTCCA TTTGGTATGA GAATCTTTCT GGTTTACGAC AGCAGACAAT GGCGGTCAAG
TTCCTTGTGG TCCTTGCTGT TGCCATTGGA CTGCCCTTCC TGGCTCTCAT TTACTGGTTT
GCTCCATGCA GCAAGATGGG GAAGATAATG CGTGGACCAT TCATGAAGTT TGTAGCACAC
GCAGCCTCCT TCACCATTTT TCTGGGACTG CTAGTCATGA ATGCAGCTGA CAGATTTGAA
GGCACAAAAC TCCTTCCTAA TGAAACCAGC ACAGATAATG CAAAACAGCT GTTCAGGATG
AAAACATCCT GCTTCTCATG GATGGAGATG CTCATTATAT CCTGGGTAAT AGGCATGATA
TGGGCTGAAT GTAAAGAAAT CTGGACTCAG GGCCCCAAGG AATATTTGTT TGAGTTGTGG
AACATGCTTG ATTTTGGTAT GTTAGCAATT TTCGCAGCAT CATTCATTGC GAGATTCATG
GCATTTTGGC ATGCTTCCAA AGCCCAGAGC ATCATTGACG CAAATGATAC TTTGAAGGAC
TTGACGAAAG TAACATTGGG AGACAATGTG AAATACTACA ATTTGGCCAG GATAAAGTGG
GACCCCTCTG ATCCTCAAAT AATATCTGAA GGTCTTTATG CAATTGCTGT AGTTTTAAGT
TTCTCTAGGA TAGCTTATAT TTTACCAGCA AATGAAAGCT TTGGACCTCT GCAGATATCA
CTTGGAAGAA CAGTCAAAGA CATCTTCAAG TTCATGGTCA TATTCATTAT GGTGTTTGTG
GCCTTTATGA TTGGAATGTT CAATCTCTAC TCCTACTACA TTGGTGCAAA ACAAAATGAA
GCCTTCACAA CAGTTGAAGA GAGTTTTAAG ACACTGTTCT GGGCTATATT TGGACTTTCT
GAAGTGAAAT CAGTGGTCAT CAACTATAAC CACAAATTCA TTGAAAACAT TGGTTACGTT
CTTTATGGAG TCTATAATGT TACGATGGTC ATTGTTTTGC TAAATATGTT AATTGCCATG
ATCAACAGTT CATTCCAGGA AATTGAGGAT GACGCTGATG TGGAGTGGAA ATTTGCAAGG
GCCAAACTCT GGTTTTCCTA CTTTGAGGAG GGCAGAACAC TTCCTGTACC CTTCAATCTG
GTGCCGAGTC CAAAGTCCCT GTTTTATCTC TTACTGAAGC TTAAAAAATG GATTTCTGAG
CTGTTCCAGG GCCATAAAAA AGGTTTCCAG GAAGATGCAG AGATGAACAA GATAAATGAA
GAAAAGAAAC TTGGAATTTT AGGAAGTCAT GAAGACCTTT CAAAATTATC ACTTGACAAA
AAACAGGTTG GGCACAATAA ACAACCAAGT ATAAGGAGCT CAGAAGATTT CCATCTAAAT
AGTTTCAATA ATCCTCCAAG ACAATATCAG AAAATAATGA AAAGGCTCAT TAAAAGATAT
GTACTGCAGG CCCAGATAGA TAAGGAGAGT GATGAAGTGA ACGAAGGGGA ACTGAAGGAA
ATTAAGCAGG ACATCTCAAG TCTCCGCTAT GAACTCCTTG AAGAAAAATC TCAGAATACA
GAAGACCTAG CAGAACTTAT TAGAGAACTT GGAGAGAAAT TATCCATGGA ACCAAATCAA
GAGGAAACCA ATAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873711.1
CDS186..2723
Translation

Target ORF information:

RefSeq Version XM_017018222.1
Organism Homo sapiens(Human)
Definition Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017018222.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGTTTAGTG ATCGCTCCAC AAGCCTATCT ATAGAGGAGG AACGCTTTTT GGATGCAGCT 
GAATATGGTA ACATCCCAGT GGTGCGGAAG ATGTTAGAAG AATGCCACTC ACTCAACGTT
AACTGTGTGG ATTACATGGG CCAGAATGCC CTACAGTTGG CAGTGGCCAA TGAGCATCTG
GAAATTACAG AACTTCTTCT CAAGAAAGAA AACCTCTCTC GAGTTGGGGA TGCTTTGCTT
CTAGCTATTA GTAAAGGTTA TGTTCGGATT GTGGAAGCAA TTCTCAGTCA TCCGGCTTTT
GCTGAAGGCA AGAGGTTAGC AACCAGCCCT AGCCAGTCTG AACTCCAGCA AGATGATTTT
TATGCCTATG ATGAAGATGG GACACGGTTC TCCCATGATG TGACTCCAAT CATTCTGGCT
GCCCACTGCC AGGAATATGA AATTGTGCAT ACCCTCCTGC GGAAGGGTGC TAGGATTGAA
CGGCCTCATG ATTATTTCTG CAAGTGCAAT GACTGCAACC AGAAACAGAA GCATGACTCG
TTTAGCCACT CCAGATCTAG GATTAATGCC TATAAAGGCC TGGCAAGTCC GGCTTACCTG
TCATTGTCTA GTGAAGATCC AGTCATGACG GCTTTAGAAC TTAGCAATGA ACTGGCAGTT
CTGGCCAATA TTGAGAAAGA GTTCAAGAAT GACTACAAAA AACTGTCAAT GCAGTGCAAA
GACTTTGTTG TTGGACTCCT TGATCTGTGC AGAAACACTG AAGAAGTCGA GGCCATTCTG
AATGGGGATG TTGAAACGCT CCAGAGTGGT GATCACGGTC GCCCAAATCT CAGCCGTTTA
AAACTTGCCA TTAAATATGA AGTAAAAAAA TTTGTAGCTC ATCCAAACTG CCAACAGCAA
CTTCTCTCCA TTTGGTATGA GAATCTTTCT GGTTTACGAC AGCAGACAAT GGCGGTCAAG
TTCCTTGTGG TCCTTGCTGT TGCCATTGGA CTGCCCTTCC TGGCTCTCAT TTACTGGTTT
GCTCCATGCA GCAAGATGGG GAAGATAATG CGTGGACCAT TCATGAAGTT TGTAGCACAC
GCAGCCTCCT TCACCATTTT TCTGGGACTG CTAGTCATGA ATGCAGCTGA CAGATTTGAA
GGCACAAAAC TCCTTCCTAA TGAAACCAGC ACAGATAATG CAAAACAGCT GTTCAGGATG
AAAACATCCT GCTTCTCATG GATGGAGATG CTCATTATAT CCTGGGTAAT AGGCATGATA
TGGGCTGAAT GTAAAGAAAT CTGGACTCAG GGCCCCAAGG AATATTTGTT TGAGTTGTGG
AACATGCTTG ATTTTGGTAT GTTAGCAATT TTCGCAGCAT CATTCATTGC GAGATTCATG
GCATTTTGGC ATGCTTCCAA AGCCCAGAGC ATCATTGACG CAAATGATAC TTTGAAGGAC
TTGACGAAAG TAACATTGGG AGACAATGTG AAATACTACA ATTTGGCCAG GATAAAGTGG
GACCCCTCTG ATCCTCAAAT AATATCTGAA GGTCTTTATG CAATTGCTGT AGTTTTAAGT
TTCTCTAGGA TAGCTTATAT TTTACCAGCA AATGAAAGCT TTGGACCTCT GCAGATATCA
CTTGGAAGAA CAGTCAAAGA CATCTTCAAG TTCATGGTCA TATTCATTAT GGTGTTTGTG
GCCTTTATGA TTGGAATGTT CAATCTCTAC TCCTACTACA TTGGTGCAAA ACAAAATGAA
GCCTTCACAA CAGTTGAAGA GAGTTTTAAG ACACTGTTCT GGGCTATATT TGGACTTTCT
GAAGTGAAAT CAGTGGTCAT CAACTATAAC CACAAATTCA TTGAAAACAT TGGTTACGTT
CTTTATGGAG TCTATAATGT TACGATGGTC ATTGTTTTGC TAAATATGTT AATTGCCATG
ATCAACAGTT CATTCCAGGA AATTGAGGAT GACGCTGATG TGGAGTGGAA ATTTGCAAGG
GCCAAACTCT GGTTTTCCTA CTTTGAGGAG GGCAGAACAC TTCCTGTACC CTTCAATCTG
GTGCCGAGTC CAAAGTCCCT GTTTTATCTC TTACTGAAGC TTAAAAAATG GATTTCTGAG
CTGTTCCAGG GCCATAAAAA AGGTTTCCAG GAAGATGCAG AGATGAACAA GATAAATGAA
GAAAAGAAAC TTGGAATTTT AGGAAGTCAT GAAGACCTTT CAAAATTATC ACTTGACAAA
AAACAGGTTG GGCACAATAA ACAACCAAGT ATAAGGAGCT CAGAAGATTT CCATCTAAAT
AGTTTCAATA ATCCTCCAAG ACAATATCAG AAAATAATGA AAAGGCTCAT TAAAAGATAT
GTACTGCAGG CCCAGATAGA TAAGGAGAGT GATGAAGTGA ACGAAGGGGA ACTGAAGGAA
ATTAAGCAGG ACATCTCAAG TCTCCGCTAT GAACTCCTTG AAGAAAAATC TCAGAATACA
GAAGACCTAG CAGAACTTAT TAGAGAACTT GGAGAGAAAT TATCCATGGA ACCAAATCAA
GAGGAAACCA ATAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61147
Clone ID Related Accession (Same CDS sequence) XM_011542968.2
Accession Version XM_011542968.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product short transient receptor potential channel 6 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAGCCGGG GATCTGACAA CAGACTGGCT CACCGGCGGC AGACAGTTCT CCGTGAGAAG 
GGGAGAAGGT TAGCTAATCG AGGACCAGCA TACATGTTTA GTGATCGCTC CACAAGCCTA
TCTATAGAGG AGGAACGCTT TTTGGATGCA GCTGAATATG GTAACATCCC AGTGGTGCGG
AAGATGTTAG AAGAATGCCA CTCACTCAAC GTTAACTGTG TGGATTACAT GGGCCAGAAT
GCCCTACAGT TGGCAGTGGC CAATGAGCAT CTGGAAATTA CAGAACTTCT TCTCAAGAAA
GAAAACCTCT CTCGAGTTGG GGATGCTTTG CTTCTAGCTA TTAGTAAAGG TTATGTTCGG
ATTGTGGAAG CAATTCTCAG TCATCCGGCT TTTGCTGAAG GCAAGAGGTT AGCAACCAGC
CCTAGCCAGT CTGAACTCCA GCAAGATGAT TTTTATGCCT ATGATGAAGA TGGGACACGG
TTCTCCCATG ATGTGACTCC AATCATTCTG GCTGCCCACT GCCAGGAATA TGAAATTGTG
CATACCCTCC TGCGGAAGGG TGCTAGGATT GAACGGCCTC ATGATTATTT CTGCAAGTGC
AATGACTGCA ACCAGAAACA GAAGCATGAC TCGTTTAGCC ACTCCAGATC TAGGATTAAT
GCCTATAAAG GCCTGGCAAG TCCGGCTTAC CTGTCATTGT CTAGTGAAGA TCCAGTCATG
ACGGCTTTAG AACTTAGCAA TGAACTGGCA GTTCTGGCCA ATATTGAGAA AGAGTTCAAG
AATGACTACA AAAAACTGTC AATGCAGTGC AAAGACTTTG TTGTTGGACT CCTTGATCTG
TGCAGAAACA CTGAAGAAGT CGAGGCCATT CTGAATGGGG ATGTTGAAAC GCTCCAGAGT
GGTGATCACG GTCGCCCAAA TCTCAGCCGT TTAAAACTTG CCATTAAATA TGAAGTAAAA
AAATTTGTAG CTCATCCAAA CTGCCAACAG CAACTTCTCT CCATTTGGTA TGAGAATCTT
TCTGGTTTAC GACAGCAGAC AATGGCGGTC AAGTTCCTTG TGGTCCTTGC TGTTGCCATT
GGACTGCCCT TCCTGGCTCT CATTTACTGG TTTGCTCCAT GCAGCAAGAT GGGGAAGATA
ATGCGTGGAC CATTCATGAA GTTTGTAGCA CACGCAGCCT CCTTCACCAT TTTTCTGGGA
CTGCTAGTCA TGAATGCAGC TGACAGATTT GAAGGCACAA AACTCCTTCC TAATGAAACC
AGCACAGATA ATGCAAAACA GCTGTTCAGG ATGAAAACAT CCTGCTTCTC ATGGATGGAG
ATGCTCATTA TATCCTGGGT AATAGGCATG ATATGGGCTG AATGTAAAGA AATCTGGACT
CAGGGCCCCA AGGAATATTT GTTTGAGTTG TGGAACATGC TTGATTTTGG TATGTTAGCA
ATTTTCGCAG CATCATTCAT TGCGAGATTC ATGGCATTTT GGCATGCTTC CAAAGCCCAG
AGCATCATTG ACGCAAATGA TACTTTGAAG GACTTGACGA AAGTAACATT GGGAGACAAT
GTGAAATACT ACAATTTGGC CAGGATAAAG TGGGACCCCT CTGATCCTCA AATAATATCT
GAAGGTCTTT ATGCAATTGC TGTAGTTTTA AGTTTCTCTA GGATAGCTTA TATTTTACCA
GCAAATGAAA GCTTTGGACC TCTGCAGATA TCACTTGGAA GAACAGTCAA AGACATCTTC
AAGTTCATGG TCATATTCAT TATGGTGTTT GTGGCCTTTA TGATTGGAAT GTTCAATCTC
TACTCCTACT ACATTGGTGC AAAACAAAAT GAAGCCTTCA CAACAGTTGA AGAGAGTTTT
AAGACACTGT TCTGGGCTAT ATTTGGACTT TCTGAAGTGA AATCAGTGGT CATCAACTAT
AACCACAAAT TCATTGAAAA CATTGGTTAC GTTCTTTATG GAGTCTATAA TGTTACGATG
GTCATTGTTT TGCTAAATAT GTTAATTGCC ATGATCAACA GTTCATTCCA GGAAATTGAG
GATGACGCTG ATGTGGAGTG GAAATTTGCA AGGGCCAAAC TCTGGTTTTC CTACTTTGAG
GAGGGCAGAA CACTTCCTGT ACCCTTCAAT CTGGTGCCGA GTCCAAAGTC CCTGTTTTAT
CTCTTACTGA AGCTTAAAAA ATGGATTTCT GAGCTGTTCC AGGGCCATAA AAAAGGTTTC
CAGGAAGATG CAGAGATGAA CAAGATAAAT GAAGAAAAGA AACTTGGAAT TTTAGGAAGT
CATGAAGACC TTTCAAAATT ATCACTTGAC AAAAAACAGG TTGGGCACAA TAAACAACCA
AGTATAAGGA GCTCAGAAGA TTTCCATCTA AATAGTTTCA ATAATCCTCC AAGACAATAT
CAGAAAATAA TGAAAAGGCT CATTAAAAGA TATGTACTGC AGGCCCAGAT AGATAAGGAG
AGTGATGAAG TGAACGAAGG GGAACTGAAG GAAATTAAGC AGGACATCTC AAGTCTCCGC
TATGAACTCC TTGAAGAAAA ATCTCAGAAT ACAGAAGACC TAGCAGAACT TATTAGAGAA
CTTGGAGAGA AATTATCCAT GGAACCAAAT CAAGAGGAAA CCAATAGATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541270.1
CDS198..2828
Misc Feature(1)276..2723
Misc Feature(2)345..749
Misc Feature(3)order(351..359,363..368,378..380,387..389,420..422,426..428,432..434,444..449,456..464,468..473,483..485,492..494,507..509,513..515,519..521,528..533,540..548,552..557,567..569,576..578)
Misc Feature(4)426..509
Misc Feature(5)513..587
Misc Feature(6)666..773
Misc Feature(7)789..968
Translation

Target ORF information:

RefSeq Version XM_011542968.2
Organism Homo sapiens(Human)
Definition Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542968.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGAGCCGGG GATCTGACAA CAGACTGGCT CACCGGCGGC AGACAGTTCT CCGTGAGAAG 
GGGAGAAGGT TAGCTAATCG AGGACCAGCA TACATGTTTA GTGATCGCTC CACAAGCCTA
TCTATAGAGG AGGAACGCTT TTTGGATGCA GCTGAATATG GTAACATCCC AGTGGTGCGG
AAGATGTTAG AAGAATGCCA CTCACTCAAC GTTAACTGTG TGGATTACAT GGGCCAGAAT
GCCCTACAGT TGGCAGTGGC CAATGAGCAT CTGGAAATTA CAGAACTTCT TCTCAAGAAA
GAAAACCTCT CTCGAGTTGG GGATGCTTTG CTTCTAGCTA TTAGTAAAGG TTATGTTCGG
ATTGTGGAAG CAATTCTCAG TCATCCGGCT TTTGCTGAAG GCAAGAGGTT AGCAACCAGC
CCTAGCCAGT CTGAACTCCA GCAAGATGAT TTTTATGCCT ATGATGAAGA TGGGACACGG
TTCTCCCATG ATGTGACTCC AATCATTCTG GCTGCCCACT GCCAGGAATA TGAAATTGTG
CATACCCTCC TGCGGAAGGG TGCTAGGATT GAACGGCCTC ATGATTATTT CTGCAAGTGC
AATGACTGCA ACCAGAAACA GAAGCATGAC TCGTTTAGCC ACTCCAGATC TAGGATTAAT
GCCTATAAAG GCCTGGCAAG TCCGGCTTAC CTGTCATTGT CTAGTGAAGA TCCAGTCATG
ACGGCTTTAG AACTTAGCAA TGAACTGGCA GTTCTGGCCA ATATTGAGAA AGAGTTCAAG
AATGACTACA AAAAACTGTC AATGCAGTGC AAAGACTTTG TTGTTGGACT CCTTGATCTG
TGCAGAAACA CTGAAGAAGT CGAGGCCATT CTGAATGGGG ATGTTGAAAC GCTCCAGAGT
GGTGATCACG GTCGCCCAAA TCTCAGCCGT TTAAAACTTG CCATTAAATA TGAAGTAAAA
AAATTTGTAG CTCATCCAAA CTGCCAACAG CAACTTCTCT CCATTTGGTA TGAGAATCTT
TCTGGTTTAC GACAGCAGAC AATGGCGGTC AAGTTCCTTG TGGTCCTTGC TGTTGCCATT
GGACTGCCCT TCCTGGCTCT CATTTACTGG TTTGCTCCAT GCAGCAAGAT GGGGAAGATA
ATGCGTGGAC CATTCATGAA GTTTGTAGCA CACGCAGCCT CCTTCACCAT TTTTCTGGGA
CTGCTAGTCA TGAATGCAGC TGACAGATTT GAAGGCACAA AACTCCTTCC TAATGAAACC
AGCACAGATA ATGCAAAACA GCTGTTCAGG ATGAAAACAT CCTGCTTCTC ATGGATGGAG
ATGCTCATTA TATCCTGGGT AATAGGCATG ATATGGGCTG AATGTAAAGA AATCTGGACT
CAGGGCCCCA AGGAATATTT GTTTGAGTTG TGGAACATGC TTGATTTTGG TATGTTAGCA
ATTTTCGCAG CATCATTCAT TGCGAGATTC ATGGCATTTT GGCATGCTTC CAAAGCCCAG
AGCATCATTG ACGCAAATGA TACTTTGAAG GACTTGACGA AAGTAACATT GGGAGACAAT
GTGAAATACT ACAATTTGGC CAGGATAAAG TGGGACCCCT CTGATCCTCA AATAATATCT
GAAGGTCTTT ATGCAATTGC TGTAGTTTTA AGTTTCTCTA GGATAGCTTA TATTTTACCA
GCAAATGAAA GCTTTGGACC TCTGCAGATA TCACTTGGAA GAACAGTCAA AGACATCTTC
AAGTTCATGG TCATATTCAT TATGGTGTTT GTGGCCTTTA TGATTGGAAT GTTCAATCTC
TACTCCTACT ACATTGGTGC AAAACAAAAT GAAGCCTTCA CAACAGTTGA AGAGAGTTTT
AAGACACTGT TCTGGGCTAT ATTTGGACTT TCTGAAGTGA AATCAGTGGT CATCAACTAT
AACCACAAAT TCATTGAAAA CATTGGTTAC GTTCTTTATG GAGTCTATAA TGTTACGATG
GTCATTGTTT TGCTAAATAT GTTAATTGCC ATGATCAACA GTTCATTCCA GGAAATTGAG
GATGACGCTG ATGTGGAGTG GAAATTTGCA AGGGCCAAAC TCTGGTTTTC CTACTTTGAG
GAGGGCAGAA CACTTCCTGT ACCCTTCAAT CTGGTGCCGA GTCCAAAGTC CCTGTTTTAT
CTCTTACTGA AGCTTAAAAA ATGGATTTCT GAGCTGTTCC AGGGCCATAA AAAAGGTTTC
CAGGAAGATG CAGAGATGAA CAAGATAAAT GAAGAAAAGA AACTTGGAAT TTTAGGAAGT
CATGAAGACC TTTCAAAATT ATCACTTGAC AAAAAACAGG TTGGGCACAA TAAACAACCA
AGTATAAGGA GCTCAGAAGA TTTCCATCTA AATAGTTTCA ATAATCCTCC AAGACAATAT
CAGAAAATAA TGAAAAGGCT CATTAAAAGA TATGTACTGC AGGCCCAGAT AGATAAGGAG
AGTGATGAAG TGAACGAAGG GGAACTGAAG GAAATTAAGC AGGACATCTC AAGTCTCCGC
TATGAACTCC TTGAAGAAAA ATCTCAGAAT ACAGAAGACC TAGCAGAACT TATTAGAGAA
CTTGGAGAGA AATTATCCAT GGAACCAAAT CAAGAGGAAA CCAATAGATA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92469
Clone ID Related Accession (Same CDS sequence) XM_017018221.1
Accession Version XM_017018221.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2448bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product short transient receptor potential channel 6 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAGCCAGA GCCCGGCGTT CGGGCCCCGG AGGGGCAGTT CTCCCCGGGG CGCTGCCGGA 
GCCGCTGCGC GGCGCAACGA GAGCCAGGAC TATCTGCTCA TGGACTCGGA GCTGGGAGAA
GACGGCTGCC CGCAAGCCCC GCTGCCTTGC TACGGCTACT ACCCCTGCTT CCGGGGATCT
GACAACAGAC TGGCTCACCG GCGGCAGACA GTTCTCCGTG AGAAGGGGAG AAGGTTAGCT
AATCGAGGAC CAGCATACAT GTTTAGTGAT CGCTCCACAA GCCTATCTAT AGAGGAGGAA
CGCTTTTTGG ATGCAGCTGA ATATGGTAAC ATCCCAGTGG TGCGGAAGAT GTTAGAAGAA
TGCCACTCAC TCAACGTTAA CTGTGTGGAT TACATGGGCC AGAATGCCCT ACAGTTGGCA
GTGGCCAATG AGCATCTGGA AATTACAGAA CTTCTTCTCA AGAAAGAAAA CCTCTCTCGA
GTTGGGGATG CTTTGCTTCT AGCTATTAGT AAAGGTTATG TTCGGATTGT GGAAGCAATT
CTCAGTCATC CGGCTTTTGC TGAAGGCAAG AGGTTAGCAA CCAGCCCTAG CCAGTCTGAA
CTCCAGCAAG ATGATTTTTA TGCCTATGAT GAAGATGGGA CACGGTTCTC CCATGATGTG
ACTCCAATCA TTCTGGCTGC CCACTGCCAG GAATATGAAA TTGTGCATAC CCTCCTGCGG
AAGGGTGCTA GGATTGAACG GCCTCATGAT TATTTCTGCA AGTGCAATGA CTGCAACCAG
AAACAGAAGC ATGACTCGTT TAGCCACTCC AGATCTAGGA TTAATGCCTA TAAAGGCCTG
GCAAGTCCGG CTTACCTGTC ATTGTCTAGT GAAGATCCAG TCATGACGGC TTTAGAACTT
AGCAATGAAC TGGCAGTTCT GGCCAATATT GAGAAAGAGT TCAAGATGGG GAAGATAATG
CGTGGACCAT TCATGAAGTT TGTAGCACAC GCAGCCTCCT TCACCATTTT TCTGGGACTG
CTAGTCATGA ATGCAGCTGA CAGATTTGAA GGCACAAAAC TCCTTCCTAA TGAAACCAGC
ACAGATAATG CAAAACAGCT GTTCAGGATG AAAACATCCT GCTTCTCATG GATGGAGATG
CTCATTATAT CCTGGGTAAT AGGCATGATA TGGGCTGAAT GTAAAGAAAT CTGGACTCAG
GGCCCCAAGG AATATTTGTT TGAGTTGTGG AACATGCTTG ATTTTGGTAT GTTAGCAATT
TTCGCAGCAT CATTCATTGC GAGATTCATG GCATTTTGGC ATGCTTCCAA AGCCCAGAGC
ATCATTGACG CAAATGATAC TTTGAAGGAC TTGACGAAAG TAACATTGGG AGACAATGTG
AAATACTACA ATTTGGCCAG GATAAAGTGG GACCCCTCTG ATCCTCAAAT AATATCTGAA
GGTCTTTATG CAATTGCTGT AGTTTTAAGT TTCTCTAGGA TAGCTTATAT TTTACCAGCA
AATGAAAGCT TTGGACCTCT GCAGATATCA CTTGGAAGAA CAGTCAAAGA CATCTTCAAG
TTCATGGTCA TATTCATTAT GGTGTTTGTG GCCTTTATGA TTGGAATGTT CAATCTCTAC
TCCTACTACA TTGGTGCAAA ACAAAATGAA GCCTTCACAA CAGTTGAAGA GAGTTTTAAG
ACACTGTTCT GGGCTATATT TGGACTTTCT GAAGTGAAAT CAGTGGTCAT CAACTATAAC
CACAAATTCA TTGAAAACAT TGGTTACGTT CTTTATGGAG TCTATAATGT TACGATGGTC
ATTGTTTTGC TAAATATGTT AATTGCCATG ATCAACAGTT CATTCCAGGA AATTGAGGAT
GACGCTGATG TGGAGTGGAA ATTTGCAAGG GCCAAACTCT GGTTTTCCTA CTTTGAGGAG
GGCAGAACAC TTCCTGTACC CTTCAATCTG GTGCCGAGTC CAAAGTCCCT GTTTTATCTC
TTACTGAAGC TTAAAAAATG GATTTCTGAG CTGTTCCAGG GCCATAAAAA AGGTTTCCAG
GAAGATGCAG AGATGAACAA GATAAATGAA GAAAAGAAAC TTGGAATTTT AGGAAGTCAT
GAAGACCTTT CAAAATTATC ACTTGACAAA AAACAGGTTG GGCACAATAA ACAACCAAGT
ATAAGGAGCT CAGAAGATTT CCATCTAAAT AGTTTCAATA ATCCTCCAAG ACAATATCAG
AAAATAATGA AAAGGCTCAT TAAAAGATAT GTACTGCAGG CCCAGATAGA TAAGGAGAGT
GATGAAGTGA ACGAAGGGGA ACTGAAGGAA ATTAAGCAGG ACATCTCAAG TCTCCGCTAT
GAACTCCTTG AAGAAAAATC TCAGAATACA GAAGACCTAG CAGAACTTAT TAGAGAACTT
GGAGAGAAAT TATCCATGGA ACCAAATCAA GAGGAAACCA ATAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873710.1
CDS516..2963
Misc Feature(1)759..2858
Misc Feature(2)828..1232
Misc Feature(3)order(834..842,846..851,861..863,870..872,903..905,909..911,915..917,927..932,939..947,951..956,966..968,975..977,990..992,996..998,1002..1004,1011..1016,1023..1031,1035..1040,1050..1052,1059..1061)
Misc Feature(4)909..992
Misc Feature(5)996..1070
Misc Feature(6)1149..1256
Misc Feature(7)1272..1451
Translation

Target ORF information:

RefSeq Version XM_017018221.1
Organism Homo sapiens(Human)
Definition Homo sapiens transient receptor potential cation channel subfamily C member 6 (TRPC6), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017018221.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGAGCCAGA GCCCGGCGTT CGGGCCCCGG AGGGGCAGTT CTCCCCGGGG CGCTGCCGGA 
GCCGCTGCGC GGCGCAACGA GAGCCAGGAC TATCTGCTCA TGGACTCGGA GCTGGGAGAA
GACGGCTGCC CGCAAGCCCC GCTGCCTTGC TACGGCTACT ACCCCTGCTT CCGGGGATCT
GACAACAGAC TGGCTCACCG GCGGCAGACA GTTCTCCGTG AGAAGGGGAG AAGGTTAGCT
AATCGAGGAC CAGCATACAT GTTTAGTGAT CGCTCCACAA GCCTATCTAT AGAGGAGGAA
CGCTTTTTGG ATGCAGCTGA ATATGGTAAC ATCCCAGTGG TGCGGAAGAT GTTAGAAGAA
TGCCACTCAC TCAACGTTAA CTGTGTGGAT TACATGGGCC AGAATGCCCT ACAGTTGGCA
GTGGCCAATG AGCATCTGGA AATTACAGAA CTTCTTCTCA AGAAAGAAAA CCTCTCTCGA
GTTGGGGATG CTTTGCTTCT AGCTATTAGT AAAGGTTATG TTCGGATTGT GGAAGCAATT
CTCAGTCATC CGGCTTTTGC TGAAGGCAAG AGGTTAGCAA CCAGCCCTAG CCAGTCTGAA
CTCCAGCAAG ATGATTTTTA TGCCTATGAT GAAGATGGGA CACGGTTCTC CCATGATGTG
ACTCCAATCA TTCTGGCTGC CCACTGCCAG GAATATGAAA TTGTGCATAC CCTCCTGCGG
AAGGGTGCTA GGATTGAACG GCCTCATGAT TATTTCTGCA AGTGCAATGA CTGCAACCAG
AAACAGAAGC ATGACTCGTT TAGCCACTCC AGATCTAGGA TTAATGCCTA TAAAGGCCTG
GCAAGTCCGG CTTACCTGTC ATTGTCTAGT GAAGATCCAG TCATGACGGC TTTAGAACTT
AGCAATGAAC TGGCAGTTCT GGCCAATATT GAGAAAGAGT TCAAGATGGG GAAGATAATG
CGTGGACCAT TCATGAAGTT TGTAGCACAC GCAGCCTCCT TCACCATTTT TCTGGGACTG
CTAGTCATGA ATGCAGCTGA CAGATTTGAA GGCACAAAAC TCCTTCCTAA TGAAACCAGC
ACAGATAATG CAAAACAGCT GTTCAGGATG AAAACATCCT GCTTCTCATG GATGGAGATG
CTCATTATAT CCTGGGTAAT AGGCATGATA TGGGCTGAAT GTAAAGAAAT CTGGACTCAG
GGCCCCAAGG AATATTTGTT TGAGTTGTGG AACATGCTTG ATTTTGGTAT GTTAGCAATT
TTCGCAGCAT CATTCATTGC GAGATTCATG GCATTTTGGC ATGCTTCCAA AGCCCAGAGC
ATCATTGACG CAAATGATAC TTTGAAGGAC TTGACGAAAG TAACATTGGG AGACAATGTG
AAATACTACA ATTTGGCCAG GATAAAGTGG GACCCCTCTG ATCCTCAAAT AATATCTGAA
GGTCTTTATG CAATTGCTGT AGTTTTAAGT TTCTCTAGGA TAGCTTATAT TTTACCAGCA
AATGAAAGCT TTGGACCTCT GCAGATATCA CTTGGAAGAA CAGTCAAAGA CATCTTCAAG
TTCATGGTCA TATTCATTAT GGTGTTTGTG GCCTTTATGA TTGGAATGTT CAATCTCTAC
TCCTACTACA TTGGTGCAAA ACAAAATGAA GCCTTCACAA CAGTTGAAGA GAGTTTTAAG
ACACTGTTCT GGGCTATATT TGGACTTTCT GAAGTGAAAT CAGTGGTCAT CAACTATAAC
CACAAATTCA TTGAAAACAT TGGTTACGTT CTTTATGGAG TCTATAATGT TACGATGGTC
ATTGTTTTGC TAAATATGTT AATTGCCATG ATCAACAGTT CATTCCAGGA AATTGAGGAT
GACGCTGATG TGGAGTGGAA ATTTGCAAGG GCCAAACTCT GGTTTTCCTA CTTTGAGGAG
GGCAGAACAC TTCCTGTACC CTTCAATCTG GTGCCGAGTC CAAAGTCCCT GTTTTATCTC
TTACTGAAGC TTAAAAAATG GATTTCTGAG CTGTTCCAGG GCCATAAAAA AGGTTTCCAG
GAAGATGCAG AGATGAACAA GATAAATGAA GAAAAGAAAC TTGGAATTTT AGGAAGTCAT
GAAGACCTTT CAAAATTATC ACTTGACAAA AAACAGGTTG GGCACAATAA ACAACCAAGT
ATAAGGAGCT CAGAAGATTT CCATCTAAAT AGTTTCAATA ATCCTCCAAG ACAATATCAG
AAAATAATGA AAAGGCTCAT TAAAAGATAT GTACTGCAGG CCCAGATAGA TAAGGAGAGT
GATGAAGTGA ACGAAGGGGA ACTGAAGGAA ATTAAGCAGG ACATCTCAAG TCTCCGCTAT
GAACTCCTTG AAGAAAAATC TCAGAATACA GAAGACCTAG CAGAACTTAT TAGAGAACTT
GGAGAGAAAT TATCCATGGA ACCAAATCAA GAGGAAACCA ATAGATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Membrane translocation of TRPC6 channels and endothelial migration are regulated by calmodulin and PI3 kinase activation.
Proceedings of the National Academy of Sciences of the United States of America113(8)2110-5(2016 Feb)
Chaudhuri P,Rosenbaum MA,Sinharoy P,Damron DS,Birnbaumer L,Graham LM


book

TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production.
Nature communications68876(2015 Nov)
Wang J,Lu R,Yang J,Li H,He Z,Jing N,Wang X,Wang Y


book

TRPC6 channel translocation into phagosomal membrane augments phagosomal function.
Proceedings of the National Academy of Sciences of the United States of America112(47)E6486-95(2015 Nov)
Riazanski V,Gabdoulkhakova AG,Boynton LS,Eguchi RR,Deriy LV,Hogarth DK,Loaëc N,Oumata N,Galons H,Brown ME,Shevchenko P,Gallan AJ,Yoo SG,Naren AP,Villereal ML,Beacham DW,Bindokas VP,Birnbaumer L,Meijer L,Nelson DJ


book

TRPC6 gene variants and neuropsychiatric lupus.
Journal of neuroimmunology28821-4(2015 Nov)
Ramirez GA,Lanzani C,Bozzolo EP,Citterio L,Zagato L,Casamassima N,Canti V,Sabbadini MG,Rovere-Querini P,Manunta P,Manfredi AA


book

TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response.
The Journal of experimental medicine212(11)1883-99(2015 Oct)
Weber EW,Han F,Tauseef M,Birnbaumer L,Mehta D,Muller WA


 
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