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TSC1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TSC1
Entrez Gene ID 7248
Full Name TSC complex subunit 1
Synonyms LAM,TSC
General protein information
Preferred Names

TSC complex subunit 1

Names

hamartin
tuberous sclerosis 1 protein
tumor suppressor

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

9

9q34.13

Summary This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009].
Disorder MIM:

605284

Disorder Html: Tuberous sclerosis-1, 191100 (3); Lymphangioleiomyomatosis, 606690

mRNA and Protein(s)

mRNA Protein Name
XM_006717271.1 XP_006717334.1 hamartin isoform X1
XM_017015099.1 XP_016870588.1 hamartin isoform X3
NM_001162427.1 NP_001155899.1 hamartin isoform 4
XM_017015101.1 XP_016870590.1 hamartin isoform X4
XM_011518979.1 XP_011517281.1 hamartin isoform X1
NM_001162426.1 NP_001155898.1 hamartin isoform 3
XM_017015096.1 XP_016870585.1 hamartin isoform X1
XM_017015098.1 XP_016870587.1 hamartin isoform X2
XM_005272211.1 XP_005272268.1 hamartin isoform X1
XM_011518979.2 XP_011517281.1 hamartin isoform X1
XM_017015097.1 XP_016870586.1 hamartin isoform X1
NM_000368.4 NP_000359.1 hamartin isoform 1
XM_017015100.1 XP_016870589.1 hamartin isoform X3



Homo sapiens (human) TSC1 NP_000359.1
Bos taurus (cattle) TSC1 XP_005213508.1
Mus musculus (house mouse) Tsc1 NP_075025.2
Xenopus tropicalis (tropical clawed frog) tsc1 XP_002944681.2
Macaca mulatta (rhesus monkey) TSC1 XP_001103846.2
Pan troglodytes (chimpanzee) TSC1 XP_001168992.1
Gallus gallus (chicken) TSC1 XP_415449.2
Canis lupus familiaris (dog) TSC1 XP_005625233.1
Rattus norvegicus (Norway rat) Tsc1 NP_068626.1
Danio rerio (zebrafish) tsc1a NP_956346.1


Related articles in PubMed

Repression of TSC1/TSC2 mediated by MeCP2 regulates human embryo lung fibroblast cell differentiation and proliferation.
Wang Y, Chen C, Deng Z, Bian E, Huang C, Lei T, Lv X, Liu L, Li J
International journal of biological macromolecules96578-588(2017 Mar)

Novel TSC1 and TSC2 gene mutations in Chinese patients with tuberous sclerosis complex.
Yu T, He Y, Li N, Zhou Y, Wang Z, Fu Q, Wang J, Wang J
Clinical neurology and neurosurgery154104-108(2017 Mar)

Tuberous sclerosis complex protein 1 expression is affected by VHL Gene alterations and HIF-1α production in sporadic clear-cell renal cell carcinoma.
Damjanovic SS, Ilic BB, Beleslin Cokic BB, Antic JA, Bankovic JZ, Milicevic IT, Rodic GS, Ilic DS, Todorovic VN, Puskas N, Tulic CD
Experimental and molecular pathology101(3)323-331(2016 Dec)

Tuberous Sclerosis Complex Associated with Vascular Anomalies or Overgrowth.
Jenkins D, McCuaig C, Drolet BA, Siegel D, Adams S, Lawson JA, Wargon O
Pediatric dermatology33(5)536-42(2016 Sep)

[Detection of TSC1/TSC2 gene mutations among patients with tuberous sclerosis complex by Ion Torrent semiconductor sequencing].
Wang Y, Lin Y, Luo C, Liang D, Ji X, Jiang T, Ma D, Xu Z
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics33(2)169-72(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings suggest the significance of previously undocumented mutation-dependent mTOR hyperactivation and frequent TSC1/2 mutations in HBV-associated HCCs. They define a molecular subset of HCC having genetic aberrations in mTOR signalling, with potential significance of effective specific drug therapy.

TSC1 mutations leading to tuberous sclerosis in Chinese children.
Title: Phenotypic and genotypic characterization of Chinese children diagnosed with tuberous sclerosis complex.

brain somatic mutations in TSC1 and TSC2 cause focal cortical dysplasia
Title: Somatic Mutations in TSC1 and TSC2 Cause Focal Cortical Dysplasia.

Repression of TSC1/TSC2 mediated by MeCP2 regulates human embryo lung fibroblast cell differentiation and proliferation.
Title: Repression of TSC1/TSC2 mediated by MeCP2 regulates human embryo lung fibroblast cell differentiation and proliferation.

Novel TSC1 mutations in Chinese patients with tuberous sclerosis.
Title: Novel TSC1 and TSC2 gene mutations in Chinese patients with tuberous sclerosis complex.

The following TSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18443 NM_000368.4
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443 XM_011518979.2
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443 XM_005272211.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443 XM_017015097.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443 XM_006717271.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu18443 XM_017015096.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu98884 XM_017015100.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 15-17 $559.30
$799.00
OHu98884 XM_017015099.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 15-17 $559.30
$799.00
OHu98885 XM_017015101.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu17591 NM_001162427.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $790.30
$1129.00
OHu17622 NM_001162426.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $825.30
$1179.00
OHu17622 XM_017015098.1
Latest version!
Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $825.30
$1179.00
View Old Accession Versions >>
OHu18443 XM_011518979.1 Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version NM_000368.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product hamartin isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070032.1, AF013168.1, D87683.1 and N63914.1. This sequence is a reference standard in the RefSeqGene project. On Jun 23, 2009 this sequence version replaced NM_000368.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013168.1, SRR1803611.75508.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000359.1
CDS235..3729
Misc Feature(1)172..174
Misc Feature(2)253..2391
Misc Feature(3)1483..1485
Misc Feature(4)1693..1695
Misc Feature(5)1747..1749
Misc Feature(6)1747..1749
Misc Feature(7)1747..1749
Misc Feature(8)1765..1767
Misc Feature(9)1795..1797
Misc Feature(10)1984..1986
Misc Feature(11)2026..2028
Misc Feature(12)2530..3030
Misc Feature(13)2569..>2877
Misc Feature(14)2821..>3105
Misc Feature(15)3373..3375
Misc Feature(16)3532..3534
Misc Feature(17)3538..3540
Exon (1)1..91
Exon (2)92..154
Exon (3)155..340
Exon (4)341..444
Exon (5)445..597
Exon (6)598..742
Exon (7)743..897
Exon (8)898..971
Exon (9)972..1147
Exon (10)1148..1263
Exon (11)1264..1375
Exon (12)1376..1497
Exon (13)1498..1567
Exon (14)1568..1672
Exon (15)1673..2231
Exon (16)2232..2275
Exon (17)2276..2442
Exon (18)2443..2625
Exon (19)2626..2736
Exon (20)2737..2859
Exon (21)2860..3047
Exon (22)3048..3209
Exon (23)3210..8616
Translation

Target ORF information:

RefSeq Version NM_000368.4
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000368.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version XM_006717271.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006717334.1
CDS215..3709
Misc Feature(1)233..2371
Misc Feature(2)2510..3010
Misc Feature(3)2549..>2857
Misc Feature(4)2801..>3085
Translation

Target ORF information:

RefSeq Version XM_006717271.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006717271.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version XM_011518979.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product hamartin isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517281.1
Translation

Target ORF information:

RefSeq Version XM_011518979.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011518979.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version XM_017015096.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870585.1
CDS694..4188
Misc Feature(1)712..2850
Misc Feature(2)2989..3489
Misc Feature(3)3028..>3336
Misc Feature(4)3280..>3564
Translation

Target ORF information:

RefSeq Version XM_017015096.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015096.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version XM_005272211.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272268.1
CDS280..3774
Misc Feature(1)298..2436
Misc Feature(2)2575..3075
Misc Feature(3)2614..>2922
Misc Feature(4)2866..>3150
Translation

Target ORF information:

RefSeq Version XM_005272211.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272211.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version XM_011518979.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011518979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517281.1
CDS229..3723
Misc Feature(1)247..2385
Misc Feature(2)2524..3024
Misc Feature(3)2563..>2871
Misc Feature(4)2815..>3099
Translation

Target ORF information:

RefSeq Version XM_011518979.2
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011518979.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18443
Clone ID Related Accession (Same CDS sequence) XM_006717271.1 , XM_011518979.1 , XM_017015096.1 , XM_005272211.1 , XM_011518979.2 , XM_017015097.1 , NM_000368.4
Accession Version XM_017015097.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870586.1
CDS665..4159
Misc Feature(1)683..2821
Misc Feature(2)2960..3460
Misc Feature(3)2999..>3307
Misc Feature(4)3251..>3535
Translation

Target ORF information:

RefSeq Version XM_017015097.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015097.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GCAGGTGGAA AAGGAACTCC TCTGGGAACC CCAGCAACCT CTCCTCCTCC AGCCCCACTC
TGTCATTCGG ATGACTACGT GCACATTTCA CTCCCCCAGG CCACAGTCAC ACCCCCCAGG
AAGGAAGAGA GAATGGATTC TGCAAGACCA TGTCTACACA GACAACACCA TCTTCTGAAT
GACAGAGGAT CAGAAGAGCC ACCTGGCAGC AAAGGTTCTG TCACTCTAAG TGATCTTCCA
GGGTTTTTAG GTGATCTGGC CTCTGAAGAA GATAGTATTG AAAAAGATAA AGAAGAAGCT
GCAATATCTA GAGAACTTTC TGAGATCACC ACAGCAGAGG CAGAGCCTGT GGTTCCTCGA
GGAGGCTTTG ACTCTCCCTT TTACCGAGAC AGTCTCCCAG GTTCTCAGCG GAAGACCCAC
TCGGCAGCCT CCAGTTCTCA GGGCGCCAGC GTGAACCCTG AGCCTTTACA CTCCTCCCTG
GACAAGCTTG GGCCTGACAC ACCAAAGCAA GCCTTTACTC CCATAGACCT GCCCTGCGGC
AGTGCTGATG AAAGCCCTGC GGGAGACAGG GAATGCCAGA CTTCTTTGGA GACCAGTATC
TTCACTCCCA GTCCTTGTAA AATTCCACCT CCGACGAGAG TGGGCTTTGG AAGCGGGCAG
CCTCCCCCGT ATGATCATCT TTTTGAGGTG GCATTGCCAA AGACAGCCCA TCATTTTGTC
ATCAGGAAGA CTGAGGAGCT GTTAAAGAAA GCAAAAGGAA ACACAGAGGA AGATGGTGTG
CCCTCTACCT CCCCAATGGA AGTGCTGGAC AGACTGATAC AGCAGGGAGC AGACGCGCAC
AGCAAGGAGC TGAACAAGTT GCCTTTACCC AGCAAGTCTG TCGACTGGAC CCACTTTGGA
GGCTCTCCTC CTTCAGATGA GATCCGCACC CTCCGAGACC AGTTGCTTTT ACTGCACAAC
CAGTTACTCT ATGAGCGTTT TAAGAGGCAG CAGCATGCCC TCCGGAACAG GCGGCTCCTC
CGCAAGGTGA TCAAAGCAGC AGCTCTGGAG GAACATAATG CTGCCATGAA AGATCAGTTG
AAGTTACAAG AGAAGGACAT CCAGATGTGG AAGGTTAGTC TGCAGAAAGA ACAAGCTAGA
TACAATCAGC TCCAGGAGCA GCGTGACACT ATGGTAACCA AGCTCCACAG CCAGATCAGA
CAGCTGCAGC ATGACCGAGA GGAATTCTAC AACCAGAGCC AGGAATTACA GACGAAGCTG
GAGGACTGCA GGAACATGAT TGCGGAGCTG CGGATAGAAC TGAAGAAGGC CAACAACAAG
GTGTGTCACA CTGAGCTGCT GCTCAGTCAG GTTTCCCAAA AGCTCTCAAA CAGTGAGTCG
GTCCAGCAGC AGATGGAGTT CTTGAACAGG CAGCTGTTGG TTCTTGGGGA GGTCAACGAG
CTCTATTTGG AACAACTGCA GAACAAGCAC TCAGATACCA CAAAGGAAGT AGAAATGATG
AAAGCCGCCT ATCGGAAAGA GCTAGAAAAA AACAGAAGCC ATGTTCTCCA GCAGACTCAG
AGGCTTGATA CCTCCCAAAA ACGGATTTTG GAACTGGAAT CTCACCTGGC CAAGAAAGAC
CACCTTCTTT TGGAACAGAA GAAATATCTA GAGGATGTCA AACTCCAGGC AAGAGGACAG
CTGCAGGCCG CAGAGAGCAG GTATGAGGCT CAGAAAAGGA TAACCCAGGT GTTTGAATTG
GAGATCTTAG ATTTATATGG CAGGTTGGAG AAAGATGGCC TCCTGAAAAA ACTTGAAGAA
GAAAAAGCAG AAGCAGCTGA AGCAGCAGAA GAAAGGCTTG ACTGTTGTAA TGACGGGTGC
TCAGATTCCA TGGTAGGGCA CAATGAAGAG GCATCTGGCC ACAACGGTGA GACCAAGACC
CCCAGGCCCA GCAGCGCCCG GGGCAGTAGT GGAAGCAGAG GTGGTGGAGG CAGCAGCAGC
AGCAGCAGCG AGCTTTCTAC CCCAGAGAAA CCCCCACACC AGAGGGCAGG CCCATTCAGC
AGTCGGTGGG AGACGACTAT GGGAGAAGCG TCTGCCAGCA TCCCCACCAC TGTGGGCTCA
CTTCCCAGTT CAAAAAGCTT CCTGGGTATG AAGGCTCGAG AGTTATTTCG TAATAAGAGC
GAGAGCCAGT GTGATGAGGA CGGCATGACC AGTAGCCTTT CTGAGAGCCT AAAGACAGAA
CTGGGCAAAG ACTTGGGTGT GGAAGCCAAG ATTCCCCTGA ACCTAGATGG CCCTCACCCG
TCTCCCCCGA CCCCGGACAG TGTTGGACAG CTACATATCA TGGACTACAA TGAGACTCAT
CATGAACACA GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98884
Clone ID Related Accession (Same CDS sequence) XM_017015099.1 , XM_017015100.1
Accession Version XM_017015099.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870588.1
CDS736..3867
Translation

Target ORF information:

RefSeq Version XM_017015099.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015099.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98884
Clone ID Related Accession (Same CDS sequence) XM_017015099.1 , XM_017015100.1
Accession Version XM_017015100.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870589.1
CDS707..3838
Translation

Target ORF information:

RefSeq Version XM_017015100.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015100.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGCA
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98885
Clone ID Related Accession (Same CDS sequence) XM_017015101.1
Accession Version XM_017015101.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGGT
GGAAAAGGAA CTCCTCTGGG AACCCCAGCA ACCTCTCCTC CTCCAGCCCC ACTCTGTCAT
TCGGATGACT ACGTGCACAT TTCACTCCCC CAGGCCACAG TCACACCCCC CAGGAAGGAA
GAGAGAATGG ATTCTGCAAG ACCATGTCTA CACAGACAAC ACCATCTTCT GAATGACAGA
GGATCAGAAG AGCCACCTGG CAGCAAAGGT TCTGTCACTC TAAGTGATCT TCCAGGGTTT
TTAGGTGATC TGGCCTCTGA AGAAGATAGT ATTGAAAAAG ATAAAGAAGA AGCTGCAATA
TCTAGAGAAC TTTCTGAGAT CACCACAGCA GAGGCAGAGC CTGTGGTTCC TCGAGGAGGC
TTTGACTCTC CCTTTTACCG AGACAGTCTC CCAGGTTCTC AGCGGAAGAC CCACTCGGCA
GCCTCCAGTT CTCAGGGCGC CAGCGTGAAC CCTGAGCCTT TACACTCCTC CCTGGACAAG
CTTGGGCCTG ACACACCAAA GCAAGCCTTT ACTCCCATAG ACCTGCCCTG CGGCAGTGCT
GATGAAAGCC CTGCGGGAGA CAGGGAATGC CAGACTTCTT TGGAGACCAG TATCTTCACT
CCCAGTCCTT GTAAAATTCC ACCTCCGACG AGAGTGGGCT TTGGAAGCGG GCAGCCTCCC
CCGTATGATC ATCTTTTTGA GGTGGCATTG CCAAAGACAG CCCATCATTT TGTCATCAGG
AAGACTGAGG AGCTGTTAAA GAAAGCAAAA GGAAACACAG AGGAAGATGG TGTGCCCTCT
ACCTCCCCAA TGGAAGTGCT GGACAGACTG ATACAGCAGG GAGCAGACGC GCACAGCAAG
GAGCTGAACA AGTTGCCTTT ACCCAGCAAG TCTGTCGACT GGACCCACTT TGGAGGCTCT
CCTCCTTCAG ATGAGATCCG CACCCTCCGA GACCAGTTGC TTTTACTGCA CAACCAGTTA
CTCTATGAGC GTTTTAAGAG GCAGCAGCAT GCCCTCCGGA ACAGGCGGCT CCTCCGCAAG
GTGATCAAAG CAGCAGCTCT GGAGGAACAT AATGCTGCCA TGAAAGATCA GTTGAAGTTA
CAAGAGAAGG ACATCCAGAT GTGGAAGGTT AGTCTGCAGA AAGAACAAGC TAGATACAAT
CAGCTCCAGG AGCAGCGTGA CACTATGGTA ACCAAGCTCC ACAGCCAGAT CAGACAGCTG
CAGCATGACC GAGAGGAATT CTACAACCAG AGCCAGGAAT TACAGACGAA GCTGGAGGAC
TGCAGGAACA TGATTGCGGA GCTGCGGATA GAACTGAAGA AGGCCAACAA CAAGGTGTGT
CACACTGAGC TGCTGCTCAG TCAGGTTTCC CAAAAGCTCT CAAACAGTGA GTCGGTCCAG
CAGCAGATGG AGTTCTTGAA CAGGCAGCTG TTGGTTCTTG GGGAGGTCAA CGAGCTCTAT
TTGGAACAAC TGCAGAACAA GCACTCAGAT ACCACAAAGG AAGTAGAAAT GATGAAAGCC
GCCTATCGGA AAGAGCTAGA AAAAAACAGA AGCCATGTTC TCCAGCAGAC TCAGAGGCTT
GATACCTCCC AAAAACGGAT TTTGGAACTG GAATCTCACC TGGCCAAGAA AGACCACCTT
CTTTTGGAAC AGAAGAAATA TCTAGAGGAT GTCAAACTCC AGGCAAGAGG ACAGCTGCAG
GCCGCAGAGA GCAGGTATGA GGCTCAGAAA AGGATAACCC AGGTGTTTGA ATTGGAGATC
TTAGATTTAT ATGGCAGGTT GGAGAAAGAT GGCCTCCTGA AAAAACTTGA AGAAGAAAAA
GCAGAAGCAG CTGAAGCAGC AGAAGAAAGG CTTGACTGTT GTAATGACGG GTGCTCAGAT
TCCATGGTAG GGCACAATGA AGAGGCATCT GGCCACAACG GTGAGACCAA GACCCCCAGG
CCCAGCAGCG CCCGGGGCAG TAGTGGAAGC AGAGGTGGTG GAGGCAGCAG CAGCAGCAGC
AGCGAGCTTT CTACCCCAGA GAAACCCCCA CACCAGAGGG CAGGCCCATT CAGCAGTCGG
TGGGAGACGA CTATGGGAGA AGCGTCTGCC AGCATCCCCA CCACTGTGGG CTCACTTCCC
AGTTCAAAAA GCTTCCTGGG TATGAAGGCT CGAGAGTTAT TTCGTAATAA GAGCGAGAGC
CAGTGTGATG AGGACGGCAT GACCAGTAGC CTTTCTGAGA GCCTAAAGAC AGAACTGGGC
AAAGACTTGG GTGTGGAAGC CAAGATTCCC CTGAACCTAG ATGGCCCTCA CCCGTCTCCC
CCGACCCCGG ACAGTGTTGG ACAGCTACAT ATCATGGACT ACAATGAGAC TCATCATGAA
CACAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870590.1
CDS737..3865
Translation

Target ORF information:

RefSeq Version XM_017015101.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015101.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGGACACTG ACGTCGTTGT CCTCACAACA GGCGTCTTGG TGTTGATAAC CATGCTACCA 
ATGATTCCAC AGTCTGGGAA ACAGCATCTT CTTGATTTCT TTGACATTTT TGGCCGTCTG
TCATCATGGT GCCTGAAGAA ACCAGGCCAC GTGGCGGAAG TCTATCTCGT CCATCTCCAT
GCCAGTGTGT ACGCACTCTT TCATCGCCTT TATGGAATGT ACCCTTGCAA CTTCGTCTCC
TTTTTGCGTT CTCATTACAG TATGAAAGAA AACCTGGAGA CTTTTGAAGA AGTGGTCAAG
CCAATGATGG AGCATGTGCG AATTCATCCG GAATTAGTGA CTGGATCCAA GGACCATGAA
CTGGACCCTC GAAGGTGGAA GAGATTAGAA ACTCATGATG TTGTGATCGA GTGTGCCAAA
ATCTCTCTGG ATCCCACAGA AGCCTCATAT GAAGATGGCT ATTCTGTGTC TCACCAAATC
TCAGCCCGCT TTCCTCATCG TTCAGCCGAT GTCACCACCA GCCCTTATGC TGACACACAG
AATAGCTATG GGTGTGCTAC TTCTACCCCT TACTCCACGT CTCGGCTGAT GTTGTTAAAT
ATGCCAGGGC AGCTACCTCA GACTCTGAGT TCCCCATCGA CACGGCTGAT AACTGAACCA
CCACAAGCTA CTCTTTGGAG CCCATCTATG GTTTGTGGTA TGACCACTCC TCCAACTTCT
CCTGGAAATG TCCCACCTGA TCTGTCACAC CCTTACAGTA AAGTCTTTGG TACAACTGGT
GGAAAAGGAA CTCCTCTGGG AACCCCAGCA ACCTCTCCTC CTCCAGCCCC ACTCTGTCAT
TCGGATGACT ACGTGCACAT TTCACTCCCC CAGGCCACAG TCACACCCCC CAGGAAGGAA
GAGAGAATGG ATTCTGCAAG ACCATGTCTA CACAGACAAC ACCATCTTCT GAATGACAGA
GGATCAGAAG AGCCACCTGG CAGCAAAGGT TCTGTCACTC TAAGTGATCT TCCAGGGTTT
TTAGGTGATC TGGCCTCTGA AGAAGATAGT ATTGAAAAAG ATAAAGAAGA AGCTGCAATA
TCTAGAGAAC TTTCTGAGAT CACCACAGCA GAGGCAGAGC CTGTGGTTCC TCGAGGAGGC
TTTGACTCTC CCTTTTACCG AGACAGTCTC CCAGGTTCTC AGCGGAAGAC CCACTCGGCA
GCCTCCAGTT CTCAGGGCGC CAGCGTGAAC CCTGAGCCTT TACACTCCTC CCTGGACAAG
CTTGGGCCTG ACACACCAAA GCAAGCCTTT ACTCCCATAG ACCTGCCCTG CGGCAGTGCT
GATGAAAGCC CTGCGGGAGA CAGGGAATGC CAGACTTCTT TGGAGACCAG TATCTTCACT
CCCAGTCCTT GTAAAATTCC ACCTCCGACG AGAGTGGGCT TTGGAAGCGG GCAGCCTCCC
CCGTATGATC ATCTTTTTGA GGTGGCATTG CCAAAGACAG CCCATCATTT TGTCATCAGG
AAGACTGAGG AGCTGTTAAA GAAAGCAAAA GGAAACACAG AGGAAGATGG TGTGCCCTCT
ACCTCCCCAA TGGAAGTGCT GGACAGACTG ATACAGCAGG GAGCAGACGC GCACAGCAAG
GAGCTGAACA AGTTGCCTTT ACCCAGCAAG TCTGTCGACT GGACCCACTT TGGAGGCTCT
CCTCCTTCAG ATGAGATCCG CACCCTCCGA GACCAGTTGC TTTTACTGCA CAACCAGTTA
CTCTATGAGC GTTTTAAGAG GCAGCAGCAT GCCCTCCGGA ACAGGCGGCT CCTCCGCAAG
GTGATCAAAG CAGCAGCTCT GGAGGAACAT AATGCTGCCA TGAAAGATCA GTTGAAGTTA
CAAGAGAAGG ACATCCAGAT GTGGAAGGTT AGTCTGCAGA AAGAACAAGC TAGATACAAT
CAGCTCCAGG AGCAGCGTGA CACTATGGTA ACCAAGCTCC ACAGCCAGAT CAGACAGCTG
CAGCATGACC GAGAGGAATT CTACAACCAG AGCCAGGAAT TACAGACGAA GCTGGAGGAC
TGCAGGAACA TGATTGCGGA GCTGCGGATA GAACTGAAGA AGGCCAACAA CAAGGTGTGT
CACACTGAGC TGCTGCTCAG TCAGGTTTCC CAAAAGCTCT CAAACAGTGA GTCGGTCCAG
CAGCAGATGG AGTTCTTGAA CAGGCAGCTG TTGGTTCTTG GGGAGGTCAA CGAGCTCTAT
TTGGAACAAC TGCAGAACAA GCACTCAGAT ACCACAAAGG AAGTAGAAAT GATGAAAGCC
GCCTATCGGA AAGAGCTAGA AAAAAACAGA AGCCATGTTC TCCAGCAGAC TCAGAGGCTT
GATACCTCCC AAAAACGGAT TTTGGAACTG GAATCTCACC TGGCCAAGAA AGACCACCTT
CTTTTGGAAC AGAAGAAATA TCTAGAGGAT GTCAAACTCC AGGCAAGAGG ACAGCTGCAG
GCCGCAGAGA GCAGGTATGA GGCTCAGAAA AGGATAACCC AGGTGTTTGA ATTGGAGATC
TTAGATTTAT ATGGCAGGTT GGAGAAAGAT GGCCTCCTGA AAAAACTTGA AGAAGAAAAA
GCAGAAGCAG CTGAAGCAGC AGAAGAAAGG CTTGACTGTT GTAATGACGG GTGCTCAGAT
TCCATGGTAG GGCACAATGA AGAGGCATCT GGCCACAACG GTGAGACCAA GACCCCCAGG
CCCAGCAGCG CCCGGGGCAG TAGTGGAAGC AGAGGTGGTG GAGGCAGCAG CAGCAGCAGC
AGCGAGCTTT CTACCCCAGA GAAACCCCCA CACCAGAGGG CAGGCCCATT CAGCAGTCGG
TGGGAGACGA CTATGGGAGA AGCGTCTGCC AGCATCCCCA CCACTGTGGG CTCACTTCCC
AGTTCAAAAA GCTTCCTGGG TATGAAGGCT CGAGAGTTAT TTCGTAATAA GAGCGAGAGC
CAGTGTGATG AGGACGGCAT GACCAGTAGC CTTTCTGAGA GCCTAAAGAC AGAACTGGGC
AAAGACTTGG GTGTGGAAGC CAAGATTCCC CTGAACCTAG ATGGCCCTCA CCCGTCTCCC
CCGACCCCGG ACAGTGTTGG ACAGCTACAT ATCATGGACT ACAATGAGAC TCATCATGAA
CACAGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17591
Clone ID Related Accession (Same CDS sequence) NM_001162427.1
Accession Version NM_001162427.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3342bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product hamartin isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI463204.1, AK303030.1, AF013168.1, D87683.1 and N63914.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001155899.1
CDS235..3576
Misc Feature(1)172..174
Misc Feature(2)253..2238
Misc Feature(3)2377..2877
Misc Feature(4)2416..>2724
Misc Feature(5)2668..>2952
Exon (1)1..91
Exon (2)92..154
Exon (3)155..340
Exon (4)341..444
Exon (5)445..589
Exon (6)590..744
Exon (7)745..818
Exon (8)819..994
Exon (9)995..1110
Exon (10)1111..1222
Exon (11)1223..1344
Exon (12)1345..1414
Exon (13)1415..1519
Exon (14)1520..2078
Exon (15)2079..2122
Exon (16)2123..2289
Exon (17)2290..2472
Exon (18)2473..2583
Exon (19)2584..2706
Exon (20)2707..2894
Exon (21)2895..3056
Exon (22)3057..8463
Translation

Target ORF information:

RefSeq Version NM_001162427.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001162427.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG ATGGACACTG ACGTCGTTGT CCTCACAACA
GGCGTCTTGG TGTTGATAAC CATGCTACCA ATGATTCCAC AGTCTGGGAA ACAGCATCTT
CTTGATTTCT TTGACATTTT TGGCCGTCTG TCATCATGGT GCCTGAAGAA ACCAGGCCAC
GTGGCGGAAG TCTATCTCGT CCATCTCCAT GCCAGTGTGT ACGCACTCTT TCATCGCCTT
TATGGAATGT ACCCTTGCAA CTTCGTCTCC TTTTTGCGTT CTCATTACAG TATGAAAGAA
AACCTGGAGA CTTTTGAAGA AGTGGTCAAG CCAATGATGG AGCATGTGCG AATTCATCCG
GAATTAGTGA CTGGATCCAA GGACCATGAA CTGGACCCTC GAAGGTGGAA GAGATTAGAA
ACTCATGATG TTGTGATCGA GTGTGCCAAA ATCTCTCTGG ATCCCACAGA AGCCTCATAT
GAAGATGGCT ATTCTGTGTC TCACCAAATC TCAGCCCGCT TTCCTCATCG TTCAGCCGAT
GTCACCACCA GCCCTTATGC TGACACACAG AATAGCTATG GGTGTGCTAC TTCTACCCCT
TACTCCACGT CTCGGCTGAT GTTGTTAAAT ATGCCAGGGC AGCTACCTCA GACTCTGAGT
TCCCCATCGA CACGGCTGAT AACTGAACCA CCACAAGCTA CTCTTTGGAG CCCATCTATG
GTTTGTGGTA TGACCACTCC TCCAACTTCT CCTGGAAATG TCCCACCTGA TCTGTCACAC
CCTTACAGTA AAGTCTTTGG TACAACTGCA GGTGGAAAAG GAACTCCTCT GGGAACCCCA
GCAACCTCTC CTCCTCCAGC CCCACTCTGT CATTCGGATG ACTACGTGCA CATTTCACTC
CCCCAGGCCA CAGTCACACC CCCCAGGAAG GAAGAGAGAA TGGATTCTGC AAGACCATGT
CTACACAGAC AACACCATCT TCTGAATGAC AGAGGATCAG AAGAGCCACC TGGCAGCAAA
GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT
AGTATTGAAA AAGATAAAGA AGAAGCTGCA ATATCTAGAG AACTTTCTGA GATCACCACA
GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA GGCTTTGACT CTCCCTTTTA CCGAGACAGT
CTCCCAGGTT CTCAGCGGAA GACCCACTCG GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG
AACCCTGAGC CTTTACACTC CTCCCTGGAC AAGCTTGGGC CTGACACACC AAAGCAAGCC
TTTACTCCCA TAGACCTGCC CTGCGGCAGT GCTGATGAAA GCCCTGCGGG AGACAGGGAA
TGCCAGACTT CTTTGGAGAC CAGTATCTTC ACTCCCAGTC CTTGTAAAAT TCCACCTCCG
ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT CCCCCGTATG ATCATCTTTT TGAGGTGGCA
TTGCCAAAGA CAGCCCATCA TTTTGTCATC AGGAAGACTG AGGAGCTGTT AAAGAAAGCA
AAAGGAAACA CAGAGGAAGA TGGTGTGCCC TCTACCTCCC CAATGGAAGT GCTGGACAGA
CTGATACAGC AGGGAGCAGA CGCGCACAGC AAGGAGCTGA ACAAGTTGCC TTTACCCAGC
AAGTCTGTCG ACTGGACCCA CTTTGGAGGC TCTCCTCCTT CAGATGAGAT CCGCACCCTC
CGAGACCAGT TGCTTTTACT GCACAACCAG TTACTCTATG AGCGTTTTAA GAGGCAGCAG
CATGCCCTCC GGAACAGGCG GCTCCTCCGC AAGGTGATCA AAGCAGCAGC TCTGGAGGAA
CATAATGCTG CCATGAAAGA TCAGTTGAAG TTACAAGAGA AGGACATCCA GATGTGGAAG
GTTAGTCTGC AGAAAGAACA AGCTAGATAC AATCAGCTCC AGGAGCAGCG TGACACTATG
GTAACCAAGC TCCACAGCCA GATCAGACAG CTGCAGCATG ACCGAGAGGA ATTCTACAAC
CAGAGCCAGG AATTACAGAC GAAGCTGGAG GACTGCAGGA ACATGATTGC GGAGCTGCGG
ATAGAACTGA AGAAGGCCAA CAACAAGGTG TGTCACACTG AGCTGCTGCT CAGTCAGGTT
TCCCAAAAGC TCTCAAACAG TGAGTCGGTC CAGCAGCAGA TGGAGTTCTT GAACAGGCAG
CTGTTGGTTC TTGGGGAGGT CAACGAGCTC TATTTGGAAC AACTGCAGAA CAAGCACTCA
GATACCACAA AGGAAGTAGA AATGATGAAA GCCGCCTATC GGAAAGAGCT AGAAAAAAAC
AGAAGCCATG TTCTCCAGCA GACTCAGAGG CTTGATACCT CCCAAAAACG GATTTTGGAA
CTGGAATCTC ACCTGGCCAA GAAAGACCAC CTTCTTTTGG AACAGAAGAA ATATCTAGAG
GATGTCAAAC TCCAGGCAAG AGGACAGCTG CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG
AAAAGGATAA CCCAGGTGTT TGAATTGGAG ATCTTAGATT TATATGGCAG GTTGGAGAAA
GATGGCCTCC TGAAAAAACT TGAAGAAGAA AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA
AGGCTTGACT GTTGTAATGA CGGGTGCTCA GATTCCATGG TAGGGCACAA TGAAGAGGCA
TCTGGCCACA ACGGTGAGAC CAAGACCCCC AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA
AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC AGCAGCGAGC TTTCTACCCC AGAGAAACCC
CCACACCAGA GGGCAGGCCC ATTCAGCAGT CGGTGGGAGA CGACTATGGG AGAAGCGTCT
GCCAGCATCC CCACCACTGT GGGCTCACTT CCCAGTTCAA AAAGCTTCCT GGGTATGAAG
GCTCGAGAGT TATTTCGTAA TAAGAGCGAG AGCCAGTGTG ATGAGGACGG CATGACCAGT
AGCCTTTCTG AGAGCCTAAA GACAGAACTG GGCAAAGACT TGGGTGTGGA AGCCAAGATT
CCCCTGAACC TAGATGGCCC TCACCCGTCT CCCCCGACCC CGGACAGTGT TGGACAGCTA
CATATCATGG ACTACAATGA GACTCATCAT GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17622
Clone ID Related Accession (Same CDS sequence) NM_001162426.1 , XM_017015098.1
Accession Version NM_001162426.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product hamartin isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA726552.1, BC108668.1, AF013168.1, D87683.1 and N63914.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.281943.1, SRR1803611.132989.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001155898.1
CDS235..3726
Misc Feature(1)172..174
Misc Feature(2)253..2388
Misc Feature(3)1690..1692
Misc Feature(4)1744..1746
Misc Feature(5)1762..1764
Misc Feature(6)1792..1794
Misc Feature(7)2023..2025
Misc Feature(8)2527..3027
Misc Feature(9)2566..>2874
Misc Feature(10)2818..>3102
Misc Feature(11)3529..3531
Exon (1)1..91
Exon (2)92..154
Exon (3)155..340
Exon (4)341..444
Exon (5)445..597
Exon (6)598..742
Exon (7)743..897
Exon (8)898..971
Exon (9)972..1147
Exon (10)1148..1263
Exon (11)1264..1375
Exon (12)1376..1494
Exon (13)1495..1564
Exon (14)1565..1669
Exon (15)1670..2228
Exon (16)2229..2272
Exon (17)2273..2439
Exon (18)2440..2622
Exon (19)2623..2733
Exon (20)2734..2856
Exon (21)2857..3044
Exon (22)3045..3206
Exon (23)3207..8613
Translation

Target ORF information:

RefSeq Version NM_001162426.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001162426.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17622
Clone ID Related Accession (Same CDS sequence) NM_001162426.1 , XM_017015098.1
Accession Version XM_017015098.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product hamartin isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016870587.1
CDS280..3771
Misc Feature(1)298..2433
Misc Feature(2)2572..3072
Misc Feature(3)2611..>2919
Misc Feature(4)2863..>3147
Translation

Target ORF information:

RefSeq Version XM_017015098.1
Organism Homo sapiens(Human)
Definition Homo sapiens tuberous sclerosis 1 (TSC1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017015098.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGGCCCAAC AAGCAAATGT CGGGGAGCTT CTTGCCATGC TGGACTCCCC CATGCTGGGT 
GTGCGGGACG ACGTGACAGC TGTCTTTAAA GAGAACCTCA ATTCTGACCG TGGCCCTATG
CTTGTAAACA CCTTGGTGGA TTATTACCTG GAAACCAGCT CTCAGCCGGC ATTGCACATC
CTGACCACCT TGCAAGAGCC ACATGACAAG CACCTCTTGG ACAGGATTAA CGAATATGTG
GGCAAAGCCG CCACTCGTTT ATCCATCCTC TCGTTACTGG GTCATGTCAT AAGACTGCAG
CCATCTTGGA AGCATAAGCT CTCTCAAGCA CCTCTTTTGC CTTCTTTACT AAAATGTCTC
AAGATGGACA CTGACGTCGT TGTCCTCACA ACAGGCGTCT TGGTGTTGAT AACCATGCTA
CCAATGATTC CACAGTCTGG GAAACAGCAT CTTCTTGATT TCTTTGACAT TTTTGGCCGT
CTGTCATCAT GGTGCCTGAA GAAACCAGGC CACGTGGCGG AAGTCTATCT CGTCCATCTC
CATGCCAGTG TGTACGCACT CTTTCATCGC CTTTATGGAA TGTACCCTTG CAACTTCGTC
TCCTTTTTGC GTTCTCATTA CAGTATGAAA GAAAACCTGG AGACTTTTGA AGAAGTGGTC
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCGGAATTAG TGACTGGATC CAAGGACCAT
GAACTGGACC CTCGAAGGTG GAAGAGATTA GAAACTCATG ATGTTGTGAT CGAGTGTGCC
AAAATCTCTC TGGATCCCAC AGAAGCCTCA TATGAAGATG GCTATTCTGT GTCTCACCAA
ATCTCAGCCC GCTTTCCTCA TCGTTCAGCC GATGTCACCA CCAGCCCTTA TGCTGACACA
CAGAATAGCT ATGGGTGTGC TACTTCTACC CCTTACTCCA CGTCTCGGCT GATGTTGTTA
AATATGCCAG GGCAGCTACC TCAGACTCTG AGTTCCCCAT CGACACGGCT GATAACTGAA
CCACCACAAG CTACTCTTTG GAGCCCATCT ATGGTTTGTG GTATGACCAC TCCTCCAACT
TCTCCTGGAA ATGTCCCACC TGATCTGTCA CACCCTTACA GTAAAGTCTT TGGTACAACT
GGTGGAAAAG GAACTCCTCT GGGAACCCCA GCAACCTCTC CTCCTCCAGC CCCACTCTGT
CATTCGGATG ACTACGTGCA CATTTCACTC CCCCAGGCCA CAGTCACACC CCCCAGGAAG
GAAGAGAGAA TGGATTCTGC AAGACCATGT CTACACAGAC AACACCATCT TCTGAATGAC
AGAGGATCAG AAGAGCCACC TGGCAGCAAA GGTTCTGTCA CTCTAAGTGA TCTTCCAGGG
TTTTTAGGTG ATCTGGCCTC TGAAGAAGAT AGTATTGAAA AAGATAAAGA AGAAGCTGCA
ATATCTAGAG AACTTTCTGA GATCACCACA GCAGAGGCAG AGCCTGTGGT TCCTCGAGGA
GGCTTTGACT CTCCCTTTTA CCGAGACAGT CTCCCAGGTT CTCAGCGGAA GACCCACTCG
GCAGCCTCCA GTTCTCAGGG CGCCAGCGTG AACCCTGAGC CTTTACACTC CTCCCTGGAC
AAGCTTGGGC CTGACACACC AAAGCAAGCC TTTACTCCCA TAGACCTGCC CTGCGGCAGT
GCTGATGAAA GCCCTGCGGG AGACAGGGAA TGCCAGACTT CTTTGGAGAC CAGTATCTTC
ACTCCCAGTC CTTGTAAAAT TCCACCTCCG ACGAGAGTGG GCTTTGGAAG CGGGCAGCCT
CCCCCGTATG ATCATCTTTT TGAGGTGGCA TTGCCAAAGA CAGCCCATCA TTTTGTCATC
AGGAAGACTG AGGAGCTGTT AAAGAAAGCA AAAGGAAACA CAGAGGAAGA TGGTGTGCCC
TCTACCTCCC CAATGGAAGT GCTGGACAGA CTGATACAGC AGGGAGCAGA CGCGCACAGC
AAGGAGCTGA ACAAGTTGCC TTTACCCAGC AAGTCTGTCG ACTGGACCCA CTTTGGAGGC
TCTCCTCCTT CAGATGAGAT CCGCACCCTC CGAGACCAGT TGCTTTTACT GCACAACCAG
TTACTCTATG AGCGTTTTAA GAGGCAGCAG CATGCCCTCC GGAACAGGCG GCTCCTCCGC
AAGGTGATCA AAGCAGCAGC TCTGGAGGAA CATAATGCTG CCATGAAAGA TCAGTTGAAG
TTACAAGAGA AGGACATCCA GATGTGGAAG GTTAGTCTGC AGAAAGAACA AGCTAGATAC
AATCAGCTCC AGGAGCAGCG TGACACTATG GTAACCAAGC TCCACAGCCA GATCAGACAG
CTGCAGCATG ACCGAGAGGA ATTCTACAAC CAGAGCCAGG AATTACAGAC GAAGCTGGAG
GACTGCAGGA ACATGATTGC GGAGCTGCGG ATAGAACTGA AGAAGGCCAA CAACAAGGTG
TGTCACACTG AGCTGCTGCT CAGTCAGGTT TCCCAAAAGC TCTCAAACAG TGAGTCGGTC
CAGCAGCAGA TGGAGTTCTT GAACAGGCAG CTGTTGGTTC TTGGGGAGGT CAACGAGCTC
TATTTGGAAC AACTGCAGAA CAAGCACTCA GATACCACAA AGGAAGTAGA AATGATGAAA
GCCGCCTATC GGAAAGAGCT AGAAAAAAAC AGAAGCCATG TTCTCCAGCA GACTCAGAGG
CTTGATACCT CCCAAAAACG GATTTTGGAA CTGGAATCTC ACCTGGCCAA GAAAGACCAC
CTTCTTTTGG AACAGAAGAA ATATCTAGAG GATGTCAAAC TCCAGGCAAG AGGACAGCTG
CAGGCCGCAG AGAGCAGGTA TGAGGCTCAG AAAAGGATAA CCCAGGTGTT TGAATTGGAG
ATCTTAGATT TATATGGCAG GTTGGAGAAA GATGGCCTCC TGAAAAAACT TGAAGAAGAA
AAAGCAGAAG CAGCTGAAGC AGCAGAAGAA AGGCTTGACT GTTGTAATGA CGGGTGCTCA
GATTCCATGG TAGGGCACAA TGAAGAGGCA TCTGGCCACA ACGGTGAGAC CAAGACCCCC
AGGCCCAGCA GCGCCCGGGG CAGTAGTGGA AGCAGAGGTG GTGGAGGCAG CAGCAGCAGC
AGCAGCGAGC TTTCTACCCC AGAGAAACCC CCACACCAGA GGGCAGGCCC ATTCAGCAGT
CGGTGGGAGA CGACTATGGG AGAAGCGTCT GCCAGCATCC CCACCACTGT GGGCTCACTT
CCCAGTTCAA AAAGCTTCCT GGGTATGAAG GCTCGAGAGT TATTTCGTAA TAAGAGCGAG
AGCCAGTGTG ATGAGGACGG CATGACCAGT AGCCTTTCTG AGAGCCTAAA GACAGAACTG
GGCAAAGACT TGGGTGTGGA AGCCAAGATT CCCCTGAACC TAGATGGCCC TCACCCGTCT
CCCCCGACCC CGGACAGTGT TGGACAGCTA CATATCATGG ACTACAATGA GACTCATCAT
GAACACAGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Repression of TSC1/TSC2 mediated by MeCP2 regulates human embryo lung fibroblast cell differentiation and proliferation.
International journal of biological macromolecules96578-588(2017 Mar)
Wang Y,Chen C,Deng Z,Bian E,Huang C,Lei T,Lv X,Liu L,Li J


book

Novel TSC1 and TSC2 gene mutations in Chinese patients with tuberous sclerosis complex.
Clinical neurology and neurosurgery154104-108(2017 Mar)
Yu T,He Y,Li N,Zhou Y,Wang Z,Fu Q,Wang J,Wang J


book

Tuberous sclerosis complex protein 1 expression is affected by VHL Gene alterations and HIF-1α production in sporadic clear-cell renal cell carcinoma.
Experimental and molecular pathology101(3)323-331(2016 Dec)
Damjanovic SS,Ilic BB,Beleslin Cokic BB,Antic JA,Bankovic JZ,Milicevic IT,Rodic GS,Ilic DS,Todorovic VN,Puskas N,Tulic CD


book

Tuberous Sclerosis Complex Associated with Vascular Anomalies or Overgrowth.
Pediatric dermatology33(5)536-42(2016 Sep)
Jenkins D,McCuaig C,Drolet BA,Siegel D,Adams S,Lawson JA,Wargon O


book

[Detection of TSC1/TSC2 gene mutations among patients with tuberous sclerosis complex by Ion Torrent semiconductor sequencing].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics33(2)169-72(2016 Apr)
Wang Y,Lin Y,Luo C,Liang D,Ji X,Jiang T,Ma D,Xu Z


 
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