×

TUBA4A cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TUBA4A
Entrez Gene ID 7277
Full Name tubulin alpha 4a
Synonyms ALS22,H2-ALPHA,TUBA1
General protein information
Preferred Names

tubulin alpha 4a

Names

tubulin alpha-4A chain
tubulin H2-alpha
tubulin alpha-1 chain
tubulin, alpha 1 (testis specific)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2q35

Summary Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013].
Disorder MIM:

191110


mRNA and Protein(s)

mRNA Protein Name
NM_006000.2 NP_005991.1 tubulin alpha-4A chain isoform 1
XM_017004824.1 XP_016860313.1 tubulin alpha-4A chain isoform X1
NM_001278552.1 NP_001265481.1 tubulin alpha-4A chain isoform 2
XM_005246832.1 XP_005246889.1 tubulin alpha-4A chain isoform X1



Homo sapiens (human) TUBA4A NP_005991.1
Danio rerio (zebrafish) tuba8l4 NP_956479.1
Macaca mulatta (rhesus monkey) TUBA4A XP_001101117.1
Bos taurus (cattle) TUBA4A NP_001071626.1
Gallus gallus (chicken) LOC100857214 XP_003641691.2
Danio rerio (zebrafish) tuba8l3 NP_001003558.1
Magnaporthe oryzae (rice blast fungus) MGG_11412 XP_003713450.1
Canis lupus familiaris (dog) TUBA4A XP_536077.2
Rattus norvegicus (Norway rat) Tuba4a NP_001007005.1
Xenopus tropicalis (tropical clawed frog) tuba4b NP_001016694.1
Mus musculus (house mouse) Tuba4a NP_033473.1
Neurospora crassa NCU09132 XP_958904.2
Pan troglodytes (chimpanzee) TUBA4A XP_003309539.1


Related articles in PubMed

Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.
Joo Y, Schumacher B, Landrieu I, Bartel M, Smet-Nocca C, Jang A, Choi HS, Jeon NL, Chang KA, Kim HS, Ottmann C, Suh YH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(10)4133-44(2015 Oct)

TUBA4A gene analysis in sporadic amyotrophic lateral sclerosis: identification of novel mutations.
Pensato V, Tiloca C, Corrado L, Bertolin C, Sardone V, Del Bo R, Calini D, Mandrioli J, Lauria G, Mazzini L, Querin G, Ceroni M, Cantello R, Corti S, Castellotti B, Soldà G, Duga S, Comi GP, Cereda C, Sorarù G, D'Alfonso S, Taroni F, Shaw CE, Landers JE, Ticozzi N, Ratti A, Gellera C, Silani V,
Journal of neurology262(5)1376-8(2015 May)

Exome-wide rare variant analysis identifies TUBA4A mutations associated with familial ALS.
Smith BN, Ticozzi N, Fallini C, Gkazi AS, Topp S, Kenna KP, Scotter EL, Kost J, Keagle P, Miller JW, Calini D, Vance C, Danielson EW, Troakes C, Tiloca C, Al-Sarraj S, Lewis EA, King A, Colombrita C, Pensato V, Castellotti B, de Belleroche J, Baas F, ten Asbroek AL, Sapp PC, McKenna-Yasek D, McLaughlin RL, Polak M, Asress S, Esteban-Pérez J, Muñoz-Blanco JL, Simpson M, , van Rheenen W, Diekstra FP, Lauria G, Duga S, Corti S, Cereda C, Corrado L, Sorarù G, Morrison KE, Williams KL, Nicholson GA, Blair IP, Dion PA, Leblond CS, Rouleau GA, Hardiman O, Veldink JH, van den Berg LH, Al-Chalabi A, Pall H, Shaw PJ, Turner MR, Talbot K, Taroni F, García-Redondo A, Wu Z, Glass JD, Gellera C, Ratti A, Brown RH Jr, Silani V, Shaw CE, Landers JE
Neuron84(2)324-31(2014 Oct)

Acetylated α-tubulin is reduced in individuals with poor sperm motility.
Bhagwat S, Dalvi V, Chandrasekhar D, Matthew T, Acharya K, Gajbhiye R, Kulkarni V, Sonawane S, Ghosalkar M, Parte P
Fertility and sterility101(1)95-104.e3(2014 Jan)

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.
Martins-de-Souza D, Guest PC, Mann DM, Roeber S, Rahmoune H, Bauder C, Kretzschmar H, Volk B, Baborie A, Bahn S
Journal of proteome research11(4)2533-43(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TUBA4A mutations are not associated with FTD.
Title: Assessing the role of TUBA4A gene in frontotemporal degeneration.

Data show that overexpression of the 14-3-3sigma isoform resulted in a disruption of the tubulin cytoskeleton mediated by binding Tau protein.
Title: Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.

This study mutational screening revealed the presence of four novel heterozygous variants of TUBA4A mutation in four sporadic amyotrophic lateral sclerosis.
Title: TUBA4A gene analysis in sporadic amyotrophic lateral sclerosis: identification of novel mutations.

In conclusion, our current results did not find an association between TUBA4A and ALS in Chinese patients, and further studies will be required.
Title: TUBA4A may not be a significant genetic factor in Chinese ALS patients.

TUBA4A mutants destabilize the microtubule network, diminishing its repolymerization capability
Title: Exome-wide rare variant analysis identifies TUBA4A mutations associated with familial ALS.

The following TUBA4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUBA4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu09800 NM_001278552.1
Latest version!
Homo sapiens tubulin alpha 4a (TUBA4A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu21787 NM_006000.2
Latest version!
Homo sapiens tubulin alpha 4a (TUBA4A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu09800 XM_005246832.1
Latest version!
Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu89852 XM_017004824.1
Latest version!
Homo sapiens tubulin alpha 4a (TUBA4A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu09800
Clone ID Related Accession (Same CDS sequence) NM_001278552.1 , XM_005246832.1
Accession Version NM_001278552.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-26
Organism Homo sapiens(Human)
Product tubulin alpha-4A chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299958.1 and AK054731.1. Transcript Variant: This variant (2) contains an alternate 5' exon, which results in translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK299958.1, BE741651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001265481.1
CDS557..1858
Misc Feature(1)101..103
Misc Feature(2)557..1855
Misc Feature(3)557..1816
Misc Feature(4)order(725..730,809..817,824..826,1052..1054,1058..1060,1181..1183)
Misc Feature(5)order(1106..1108,1268..1273,1277..1285,1289..1294,1487..1489,1565..1567)
Exon (1)1..514
Exon (2)515..737
Exon (3)738..886
Exon (4)887..2499
Translation

Target ORF information:

RefSeq Version NM_001278552.1
Organism Homo sapiens(Human)
Definition Homo sapiens tubulin alpha 4a (TUBA4A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001278552.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21787
Clone ID Related Accession (Same CDS sequence) NM_006000.2
Accession Version NM_006000.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-26
Organism Homo sapiens(Human)
Product tubulin alpha-4A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP346611.1 and AK054731.1. This sequence is a reference standard in the RefSeqGene project. On Jun 21, 2013 this sequence version replaced NM_006000.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK054731.1, BC009238.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAAT GCATCTCAGT CCACGTGGGG CAGGCAGGTG TCCAGATGGG CAATGCCTGC 
TGGGAGCTCT ATTGCTTGGA ACATGGGATT CAGCCTGATG GGCAGATGCC CAGTGACAAG
ACCATTGGTG GAGGGGACGA CTCCTTCACC ACCTTCTTCT GTGAAACTGG TGCTGGAAAA
CACGTACCCC GGGCAGTTTT TGTGGATCTG GAGCCTACGG TCATTGATGA GATCCGAAAT
GGCCCATACC GACAGCTCTT CCACCCAGAG CAGCTCATCA CTGGGAAAGA GGATGCTGCC
AACAACTATG CCCGTGGTCA CTATACCATT GGCAAGGAGA TCATTGACCC AGTGCTGGAT
CGGATCCGCA AGCTGTCTGA CCAGTGCACA GGACTTCAGG GCTTCCTGGT GTTCCACAGC
TTTGGTGGGG GCACTGGCTC TGGCTTCACC TCACTCCTGA TGGAGCGGCT CTCTGTTGAC
TATGGCAAGA AATCCAAGCT GGAATTCTCC ATCTACCCAG CCCCCCAGGT GTCTACAGCC
GTGGTCGAGC CCTACAACTC TATCCTGACC ACCCACACCA CCCTGGAGCA CTCAGACTGT
GCCTTCATGG TGGACAACGA AGCAATCTAT GACATCTGCC GCCGCAACCT AGACATCGAG
CGCCCAACCT ACACCAACCT CAATCGCCTC ATTAGCCAAA TTGTCTCCTC CATCACAGCT
TCTCTGCGCT TTGACGGGGC CCTCAATGTG GACCTGACAG AGTTCCAGAC CAACCTGGTG
CCCTACCCTC GCATCCACTT CCCCCTGGCC ACCTATGCAC CAGTCATCTC TGCAGAAAAG
GCATACCACG AGCAGCTGTC GGTGGCAGAG ATCACCAATG CCTGCTTTGA GCCTGCCAAC
CAGATGGTAA AGTGTGATCC CCGGCACGGC AAGTACATGG CCTGCTGCCT GCTGTACCGT
GGAGATGTGG TGCCCAAGGA TGTCAACGCT GCCATTGCCG CCATCAAGAC CAAGCGCAGC
ATTCAGTTTG TGGACTGGTG CCCCACAGGC TTCAAGGTTG GTATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGGGGTGA CCTGGCCAAG GTGCAGCGTG CCGTGTGCAT GCTGAGCAAC
ACGACCGCCA TCGCCGAGGC CTGGGCCCGC CTGGACCACA AGTTCGACCT GATGTATGCC
AAGAGGGCGT TTGTGCACTG GTATGTGGGT GAGGGCATGG AGGAGGGTGA GTTCTCCGAG
GCCCGTGAGG ATATGGCTGC CCTGGAGAAG GATTATGAGG AGGTGGGCAT CGACTCCTAT
GAGGACGAGG ATGAGGGAGA AGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005991.1
CDS89..1435
Misc Feature(1)65..67
Misc Feature(2)89..1432
Misc Feature(3)92..1393
Misc Feature(4)order(92..94,683..685,845..850,854..862,866..871,1064..1066,1142..1144)
Misc Feature(5)order(119..124,131..133,299..301,389..391,515..526,599..601,704..706,758..760,770..772)
Misc Feature(6)206..208
Misc Feature(7)206..208
Misc Feature(8)230..232
Misc Feature(9)230..232
Misc Feature(10)order(302..307,386..394,401..403,629..631,635..637,758..760)
Misc Feature(11)335..337
Misc Feature(12)902..904
Misc Feature(13)1157..1159
Misc Feature(14)1382..1384
Misc Feature(15)1382..1384
Misc Feature(16)1403..1405
Exon (1)1..91
Exon (2)92..314
Exon (3)315..463
Exon (4)464..2076
Translation

Target ORF information:

RefSeq Version NM_006000.2
Organism Homo sapiens(Human)
Definition Homo sapiens tubulin alpha 4a (TUBA4A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006000.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCGTGAAT GCATCTCAGT CCACGTGGGG CAGGCAGGTG TCCAGATGGG CAATGCCTGC 
TGGGAGCTCT ATTGCTTGGA ACATGGGATT CAGCCTGATG GGCAGATGCC CAGTGACAAG
ACCATTGGTG GAGGGGACGA CTCCTTCACC ACCTTCTTCT GTGAAACTGG TGCTGGAAAA
CACGTACCCC GGGCAGTTTT TGTGGATCTG GAGCCTACGG TCATTGATGA GATCCGAAAT
GGCCCATACC GACAGCTCTT CCACCCAGAG CAGCTCATCA CTGGGAAAGA GGATGCTGCC
AACAACTATG CCCGTGGTCA CTATACCATT GGCAAGGAGA TCATTGACCC AGTGCTGGAT
CGGATCCGCA AGCTGTCTGA CCAGTGCACA GGACTTCAGG GCTTCCTGGT GTTCCACAGC
TTTGGTGGGG GCACTGGCTC TGGCTTCACC TCACTCCTGA TGGAGCGGCT CTCTGTTGAC
TATGGCAAGA AATCCAAGCT GGAATTCTCC ATCTACCCAG CCCCCCAGGT GTCTACAGCC
GTGGTCGAGC CCTACAACTC TATCCTGACC ACCCACACCA CCCTGGAGCA CTCAGACTGT
GCCTTCATGG TGGACAACGA AGCAATCTAT GACATCTGCC GCCGCAACCT AGACATCGAG
CGCCCAACCT ACACCAACCT CAATCGCCTC ATTAGCCAAA TTGTCTCCTC CATCACAGCT
TCTCTGCGCT TTGACGGGGC CCTCAATGTG GACCTGACAG AGTTCCAGAC CAACCTGGTG
CCCTACCCTC GCATCCACTT CCCCCTGGCC ACCTATGCAC CAGTCATCTC TGCAGAAAAG
GCATACCACG AGCAGCTGTC GGTGGCAGAG ATCACCAATG CCTGCTTTGA GCCTGCCAAC
CAGATGGTAA AGTGTGATCC CCGGCACGGC AAGTACATGG CCTGCTGCCT GCTGTACCGT
GGAGATGTGG TGCCCAAGGA TGTCAACGCT GCCATTGCCG CCATCAAGAC CAAGCGCAGC
ATTCAGTTTG TGGACTGGTG CCCCACAGGC TTCAAGGTTG GTATCAACTA CCAGCCTCCC
ACTGTGGTGC CTGGGGGTGA CCTGGCCAAG GTGCAGCGTG CCGTGTGCAT GCTGAGCAAC
ACGACCGCCA TCGCCGAGGC CTGGGCCCGC CTGGACCACA AGTTCGACCT GATGTATGCC
AAGAGGGCGT TTGTGCACTG GTATGTGGGT GAGGGCATGG AGGAGGGTGA GTTCTCCGAG
GCCCGTGAGG ATATGGCTGC CCTGGAGAAG GATTATGAGG AGGTGGGCAT CGACTCCTAT
GAGGACGAGG ATGAGGGAGA AGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09800
Clone ID Related Accession (Same CDS sequence) NM_001278552.1 , XM_005246832.1
Accession Version XM_005246832.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1302bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product tubulin alpha-4A chain isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246889.1
Translation

Target ORF information:

RefSeq Version XM_005246832.1
Organism Homo sapiens(Human)
Definition Homo sapiens tubulin, alpha 4a (TUBA4A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005246832.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGGCAATG CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG 
ATGCCCAGTG ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA
ACTGGTGCTG GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT
GATGAGATCC GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG
AAAGAGGATG CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT
GACCCAGTGC TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC
CTGGTGTTCC ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG
CGGCTCTCTG TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC
CAGGTGTCTA CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG
GAGCACTCAG ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC
AACCTAGACA TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC
TCCTCCATCA CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC
CAGACCAACC TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC
ATCTCTGCAG AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC
TTTGAGCCTG CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC
TGCCTGCTGT ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC
AAGACCAAGC GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC
AACTACCAGC CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG
TGCATGCTGA GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC
GACCTGATGT ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG
GGTGAGTTCT CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG
GGCATCGACT CCTATGAGGA CGAGGATGAG GGAGAAGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89852
Clone ID Related Accession (Same CDS sequence) XM_017004824.1
Accession Version XM_017004824.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product tubulin alpha-4A chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGCGGC TCTCTGTTGA CTATGGCAAG AAATCCAAGC TGGAATTCTC CATCTACCCA 
GCCCCCCAGG TGTCTACAGC CGTGGTCGAG CCCTACAACT CTATCCTGAC CACCCACACC
ACCCTGGAGC ACTCAGACTG TGCCTTCATG GTGGACAACG AAGCAATCTA TGACATCTGC
CGCCGCAACC TAGACATCGA GCGCCCAACC TACACCAACC TCAATCGCCT CATTAGCCAA
ATTGTCTCCT CCATCACAGC TTCTCTGCGC TTTGACGGGG CCCTCAATGT GGACCTGACA
GAGTTCCAGA CCAACCTGGT GCCCTACCCT CGCATCCACT TCCCCCTGGC CACCTATGCA
CCAGTCATCT CTGCAGAAAA GGCATACCAC GAGCAGCTGT CGGTGGCAGA GATCACCAAT
GCCTGCTTTG AGCCTGCCAA CCAGATGGTA AAGTGTGATC CCCGGCACGG CAAGTACATG
GCCTGCTGCC TGCTGTACCG TGGAGATGTG GTGCCCAAGG ATGTCAACGC TGCCATTGCC
GCCATCAAGA CCAAGCGCAG CATTCAGTTT GTGGACTGGT GCCCCACAGG CTTCAAGGTT
GGTATCAACT ACCAGCCTCC CACTGTGGTG CCTGGGGGTG ACCTGGCCAA GGTGCAGCGT
GCCGTGTGCA TGCTGAGCAA CACGACCGCC ATCGCCGAGG CCTGGGCCCG CCTGGACCAC
AAGTTCGACC TGATGTATGC CAAGAGGGCG TTTGTGCACT GGTATGTGGG TGAGGGCATG
GAGGAGGGTG AGTTCTCCGA GGCCCGTGAG GATATGGCTG CCCTGGAGAA GGATTATGAG
GAGGTGGGCA TCGACTCCTA TGAGGACGAG GATGAGGGAG AAGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860313.1
CDS295..1182
Misc Feature(1)<295..1140
Misc Feature(2)order(430..432,592..597,601..609,613..618,811..813,889..891)
Translation

Target ORF information:

RefSeq Version XM_017004824.1
Organism Homo sapiens(Human)
Definition Homo sapiens tubulin alpha 4a (TUBA4A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017004824.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGCGGC TCTCTGTTGA CTATGGCAAG AAATCCAAGC TGGAATTCTC CATCTACCCA 
GCCCCCCAGG TGTCTACAGC CGTGGTCGAG CCCTACAACT CTATCCTGAC CACCCACACC
ACCCTGGAGC ACTCAGACTG TGCCTTCATG GTGGACAACG AAGCAATCTA TGACATCTGC
CGCCGCAACC TAGACATCGA GCGCCCAACC TACACCAACC TCAATCGCCT CATTAGCCAA
ATTGTCTCCT CCATCACAGC TTCTCTGCGC TTTGACGGGG CCCTCAATGT GGACCTGACA
GAGTTCCAGA CCAACCTGGT GCCCTACCCT CGCATCCACT TCCCCCTGGC CACCTATGCA
CCAGTCATCT CTGCAGAAAA GGCATACCAC GAGCAGCTGT CGGTGGCAGA GATCACCAAT
GCCTGCTTTG AGCCTGCCAA CCAGATGGTA AAGTGTGATC CCCGGCACGG CAAGTACATG
GCCTGCTGCC TGCTGTACCG TGGAGATGTG GTGCCCAAGG ATGTCAACGC TGCCATTGCC
GCCATCAAGA CCAAGCGCAG CATTCAGTTT GTGGACTGGT GCCCCACAGG CTTCAAGGTT
GGTATCAACT ACCAGCCTCC CACTGTGGTG CCTGGGGGTG ACCTGGCCAA GGTGCAGCGT
GCCGTGTGCA TGCTGAGCAA CACGACCGCC ATCGCCGAGG CCTGGGCCCG CCTGGACCAC
AAGTTCGACC TGATGTATGC CAAGAGGGCG TTTGTGCACT GGTATGTGGG TGAGGGCATG
GAGGAGGGTG AGTTCTCCGA GGCCCGTGAG GATATGGCTG CCCTGGAGAA GGATTATGAG
GAGGTGGGCA TCGACTCCTA TGAGGACGAG GATGAGGGAG AAGAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Involvement of 14-3-3 in tubulin instability and impaired axon development is mediated by Tau.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology29(10)4133-44(2015 Oct)
Joo Y,Schumacher B,Landrieu I,Bartel M,Smet-Nocca C,Jang A,Choi HS,Jeon NL,Chang KA,Kim HS,Ottmann C,Suh YH


book

TUBA4A gene analysis in sporadic amyotrophic lateral sclerosis: identification of novel mutations.
Journal of neurology262(5)1376-8(2015 May)
Pensato V,Tiloca C,Corrado L,Bertolin C,Sardone V,Del Bo R,Calini D,Mandrioli J,Lauria G,Mazzini L,Querin G,Ceroni M,Cantello R,Corti S,Castellotti B,Soldà G,Duga S,Comi GP,Cereda C,Sorarù G,D'Alfonso S,Taroni F,Shaw CE,Landers JE,Ticozzi N,Ratti A,Gellera C,Silani V,


book

Exome-wide rare variant analysis identifies TUBA4A mutations associated with familial ALS.
Neuron84(2)324-31(2014 Oct)
Smith BN,Ticozzi N,Fallini C,Gkazi AS,Topp S,Kenna KP,Scotter EL,Kost J,Keagle P,Miller JW,Calini D,Vance C,Danielson EW,Troakes C,Tiloca C,Al-Sarraj S,Lewis EA,King A,Colombrita C,Pensato V,Castellotti B,de Belleroche J,Baas F,ten Asbroek AL,Sapp PC,McKenna-Yasek D,McLaughlin RL,Polak M,Asress S,Esteban-Pérez J,Muñoz-Blanco JL,Simpson M,,van Rheenen W,Diekstra FP,Lauria G,Duga S,Corti S,Cereda C,Corrado L,Sorarù G,Morrison KE,Williams KL,Nicholson GA,Blair IP,Dion PA,Leblond CS,Rouleau GA,Hardiman O,Veldink JH,van den Berg LH,Al-Chalabi A,Pall H,Shaw PJ,Turner MR,Talbot K,Taroni F,García-Redondo A,Wu Z,Glass JD,Gellera C,Ratti A,Brown RH Jr,Silani V,Shaw CE,Landers JE


book

Acetylated α-tubulin is reduced in individuals with poor sperm motility.
Fertility and sterility101(1)95-104.e3(2014 Jan)
Bhagwat S,Dalvi V,Chandrasekhar D,Matthew T,Acharya K,Gajbhiye R,Kulkarni V,Sonawane S,Ghosalkar M,Parte P


book

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.
Journal of proteome research11(4)2533-43(2012 Apr)
Martins-de-Souza D,Guest PC,Mann DM,Roeber S,Rahmoune H,Bauder C,Kretzschmar H,Volk B,Baborie A,Bahn S


 
*
*
*
*