×

WAS cDNA ORF clone, Homo sapiens (human)

Gene Symbol WAS
Entrez Gene ID 7454
Full Name Wiskott-Aldrich syndrome
Synonyms IMD2, SCNX, THC, THC1, WASP, WASPA
General protein information
Preferred Names
wiskott-Aldrich syndrome protein
Names
wiskott-Aldrich syndrome protein
eczema-thrombocytopenia
thrombocytopenia 1 (X-linked)
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.4-p11.21

Summary The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

300392

Disorder Html: Wiskott-Aldrich syndrome, 301000 (3);

mRNA and Protein(s)

mRNA Protein Name
XM_011543977 XP_011542279 wiskott-Aldrich syndrome protein isoform X1
NM_000377 NP_000368 wiskott-Aldrich syndrome protein



Homo sapiens (human) WAS NP_000368.1
Macaca mulatta (Rhesus monkey) LOC714474 XP_001104246.2
Canis lupus familiaris (dog) WAS XP_548986.2
Mus musculus (house mouse) Was NP_033541.1
Rattus norvegicus (Norway rat) Was NP_001101718.1
Danio rerio (zebrafish) wasa XP_699919.5


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Free ORF Clone
for Every Scientist
More Detail >>

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu61316 XM_011543977 PREDICTED: Homo sapiens Wiskott-Aldrich syndrome (WAS), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu19178
NM_000377 Homo sapiens Wiskott-Aldrich syndrome (WAS), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu61316
Clone ID Related Accession (Same CDS sequence) XM_011543977
Accession Version XM_011543977.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1353bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product wiskott-Aldrich syndrome protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTGGGG GCCCAATGGG AGGAAGGCCC GGGGGCCGAG GAGCACCAGC GGTTCAGCAG 
AACATACCCT CCACCCTCCT CCAGGACCAC GAGAACCAGC GACTCTTTGA GATGCTTGGA
CGAAAATGCT TGACGCTGGC CACTGCAGTT GTTCAGCTGT ACCTGGCGCT GCCCCCTGGA
GCTGAGCACT GGACCAAGGA GCATTGTGGG GCTGTGTGCT TCGTGAAGGA TAACCCCCAG
AAGTCCTACT TCATCCGCCT TTACGGCCTT CAGGCTGGTC GGCTGCTCTG GGAACAGGAG
CTGTACTCAC AGCTTGTCTA CTCCACCCCC ACCCCCTTCT TCCACACCTT CGCTGGAGAT
GACTGCCAAG CGGGGCTGAA CTTTGCAGAC GAGGACGAGG CCCAGGCCTT CCGGGCCCTC
GTGCAGGAGA AGATACAAAA AAGGAATCAG AGGCAAAGTG GAGACAGACG CCAGCTACCC
CCACCACCAA CACCAGCCAA TGAAGAGAGA AGAGGAGGGC TCCCACCCCT GCCCCTGCAT
CCAGGTGGAG ACCAAGGAGG CCCTCCAGTG GGTCCGCTCT CCCTGGGGCT GGCGACAGTG
GACATCCAGA ACCCTGACAT CACGAGTTCA CGATACCGTG GGCTCCCAGC ACCTGGACCT
AGCCCAGCTG ATAAGAAACG CTCAGGGAAG AAGAAGATCA GCAAAGCTGA TATTGGTGCA
CCCAGTGGAT TCAAGCATGT CAGCCACGTG GGGTGGGACC CCCAGAATGG ATTTGACGTG
AACAACCTCG ACCCAGATCT GCGGAGTCTG TTCTCCAGGG CAGGAATCAG CGAGGCCCAG
CTCACCGACG CCGAGACCTC TAAACTTATC TACGACTTCA TTGAGGACCA GGGTGGGCTG
GAGGCTGTGC GGCAGGAGAT GAGGCGCCAG GGCCGAGGGG GCCCTCCACC ACCACCCCCT
CCAGCTACTG GACGTTCTGG ACCACTGCCC CCTCCACCCC CTGGAGCTGG TGGGCCACCC
ATGCCACCAC CACCGCCACC ACCGCCACCG CCGCCCAGCT CCGGGAATGG ACCAGCCCCT
CCCCCACTCC CTCCTGCTCT GGTGCCTGCC GGGGGCCTGG CCCCTGGTGG GGGTCGGGGA
GCGCTTTTGG ATCAAATCCG GCAGGGAATT CAGCTGAACA AGACCCCTGG GGCCCCAGAG
AGCTCAGCGC TGCAGCCACC ACCTCAGAGC TCAGAGGGAC TGGTGGGGGC CCTGATGCAC
GTGATGCAGA AGAGAAGCAG AGCCATCCAC TCCTCCGACG AAGGGGAGGA CCAGGCTGGC
GATGAAGATG AAGATGATGA ATGGGATGAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542279.1
CDS65..1417
Misc Feature(1)197..499(+)
Misc Feature(2)197..439(+)
Misc Feature(3)224..412(+)
Misc Feature(4)770..898(+)
Misc Feature(5)776..877(+)
Translation

Target ORF information:

RefSeq Version XM_011543977
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Wiskott-Aldrich syndrome (WAS), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543977

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGAGTGGGG GCCCAATGGG AGGAAGGCCC GGGGGCCGAG GAGCACCAGC GGTTCAGCAG 
AACATACCCT CCACCCTCCT CCAGGACCAC GAGAACCAGC GACTCTTTGA GATGCTTGGA
CGAAAATGCT TGACGCTGGC CACTGCAGTT GTTCAGCTGT ACCTGGCGCT GCCCCCTGGA
GCTGAGCACT GGACCAAGGA GCATTGTGGG GCTGTGTGCT TCGTGAAGGA TAACCCCCAG
AAGTCCTACT TCATCCGCCT TTACGGCCTT CAGGCTGGTC GGCTGCTCTG GGAACAGGAG
CTGTACTCAC AGCTTGTCTA CTCCACCCCC ACCCCCTTCT TCCACACCTT CGCTGGAGAT
GACTGCCAAG CGGGGCTGAA CTTTGCAGAC GAGGACGAGG CCCAGGCCTT CCGGGCCCTC
GTGCAGGAGA AGATACAAAA AAGGAATCAG AGGCAAAGTG GAGACAGACG CCAGCTACCC
CCACCACCAA CACCAGCCAA TGAAGAGAGA AGAGGAGGGC TCCCACCCCT GCCCCTGCAT
CCAGGTGGAG ACCAAGGAGG CCCTCCAGTG GGTCCGCTCT CCCTGGGGCT GGCGACAGTG
GACATCCAGA ACCCTGACAT CACGAGTTCA CGATACCGTG GGCTCCCAGC ACCTGGACCT
AGCCCAGCTG ATAAGAAACG CTCAGGGAAG AAGAAGATCA GCAAAGCTGA TATTGGTGCA
CCCAGTGGAT TCAAGCATGT CAGCCACGTG GGGTGGGACC CCCAGAATGG ATTTGACGTG
AACAACCTCG ACCCAGATCT GCGGAGTCTG TTCTCCAGGG CAGGAATCAG CGAGGCCCAG
CTCACCGACG CCGAGACCTC TAAACTTATC TACGACTTCA TTGAGGACCA GGGTGGGCTG
GAGGCTGTGC GGCAGGAGAT GAGGCGCCAG GGCCGAGGGG GCCCTCCACC ACCACCCCCT
CCAGCTACTG GACGTTCTGG ACCACTGCCC CCTCCACCCC CTGGAGCTGG TGGGCCACCC
ATGCCACCAC CACCGCCACC ACCGCCACCG CCGCCCAGCT CCGGGAATGG ACCAGCCCCT
CCCCCACTCC CTCCTGCTCT GGTGCCTGCC GGGGGCCTGG CCCCTGGTGG GGGTCGGGGA
GCGCTTTTGG ATCAAATCCG GCAGGGAATT CAGCTGAACA AGACCCCTGG GGCCCCAGAG
AGCTCAGCGC TGCAGCCACC ACCTCAGAGC TCAGAGGGAC TGGTGGGGGC CCTGATGCAC
GTGATGCAGA AGAGAAGCAG AGCCATCCAC TCCTCCGACG AAGGGGAGGA CCAGGCTGGC
GATGAAGATG AAGATGATGA ATGGGATGAC TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19178
Clone ID Related Accession (Same CDS sequence) NM_000377
Accession Version NM_000377.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1509bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 28-APR-2015
Organism Homo sapiens (human)
Product wiskott-Aldrich syndrome protein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI910072.1, U19927.1 and CF529565.1. This sequence is a reference standard in the RefSeqGene project. On May 7, 2008 this sequence version replaced gi:4507908. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002961.1, U12707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTGGGG GCCCAATGGG AGGAAGGCCC GGGGGCCGAG GAGCACCAGC GGTTCAGCAG 
AACATACCCT CCACCCTCCT CCAGGACCAC GAGAACCAGC GACTCTTTGA GATGCTTGGA
CGAAAATGCT TGACGCTGGC CACTGCAGTT GTTCAGCTGT ACCTGGCGCT GCCCCCTGGA
GCTGAGCACT GGACCAAGGA GCATTGTGGG GCTGTGTGCT TCGTGAAGGA TAACCCCCAG
AAGTCCTACT TCATCCGCCT TTACGGCCTT CAGGCTGGTC GGCTGCTCTG GGAACAGGAG
CTGTACTCAC AGCTTGTCTA CTCCACCCCC ACCCCCTTCT TCCACACCTT CGCTGGAGAT
GACTGCCAAG CGGGGCTGAA CTTTGCAGAC GAGGACGAGG CCCAGGCCTT CCGGGCCCTC
GTGCAGGAGA AGATACAAAA AAGGAATCAG AGGCAAAGTG GAGACAGACG CCAGCTACCC
CCACCACCAA CACCAGCCAA TGAAGAGAGA AGAGGAGGGC TCCCACCCCT GCCCCTGCAT
CCAGGTGGAG ACCAAGGAGG CCCTCCAGTG GGTCCGCTCT CCCTGGGGCT GGCGACAGTG
GACATCCAGA ACCCTGACAT CACGAGTTCA CGATACCGTG GGCTCCCAGC ACCTGGACCT
AGCCCAGCTG ATAAGAAACG CTCAGGGAAG AAGAAGATCA GCAAAGCTGA TATTGGTGCA
CCCAGTGGAT TCAAGCATGT CAGCCACGTG GGGTGGGACC CCCAGAATGG ATTTGACGTG
AACAACCTCG ACCCAGATCT GCGGAGTCTG TTCTCCAGGG CAGGAATCAG CGAGGCCCAG
CTCACCGACG CCGAGACCTC TAAACTTATC TACGACTTCA TTGAGGACCA GGGTGGGCTG
GAGGCTGTGC GGCAGGAGAT GAGGCGCCAG GAGCCACTTC CGCCGCCCCC ACCGCCATCT
CGAGGAGGGA ACCAGCTCCC CCGGCCCCCT ATTGTGGGGG GTAACAAGGG TCGTTCTGGT
CCACTGCCCC CTGTACCTTT GGGGATTGCC CCACCCCCAC CAACACCCCG GGGACCCCCA
CCCCCAGGCC GAGGGGGCCC TCCACCACCA CCCCCTCCAG CTACTGGACG TTCTGGACCA
CTGCCCCCTC CACCCCCTGG AGCTGGTGGG CCACCCATGC CACCACCACC GCCACCACCG
CCACCGCCGC CCAGCTCCGG GAATGGACCA GCCCCTCCCC CACTCCCTCC TGCTCTGGTG
CCTGCCGGGG GCCTGGCCCC TGGTGGGGGT CGGGGAGCGC TTTTGGATCA AATCCGGCAG
GGAATTCAGC TGAACAAGAC CCCTGGGGCC CCAGAGAGCT CAGCGCTGCA GCCACCACCT
CAGAGCTCAG AGGGACTGGT GGGGGCCCTG ATGCACGTGA TGCAGAAGAG AAGCAGAGCC
ATCCACTCCT CCGACGAAGG GGAGGACCAG GCTGGCGATG AAGATGAAGA TGATGAATGG
GATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000368.1
CDS58..1566
Misc Feature(1)190..492(+)
Misc Feature(2)190..432(+)
Misc Feature(3)217..405(+)
Misc Feature(4)763..891(+)
Misc Feature(5)769..870(+)
Misc Feature(6)928..930(+)
Misc Feature(7)928..930(+)
Misc Feature(8)928..930(+)
Misc Feature(9)928..930(+)
Misc Feature(10)1066..1095(+)
Misc Feature(11)1183..1212(+)
Misc Feature(12)1504..1506(+)
Misc Feature(13)1504..1506(+)
Misc Feature(14)1504..1506(+)
Misc Feature(15)1507..1509(+)
Misc Feature(16)1507..1509(+)
Misc Feature(17)1507..1509(+)
Exon (1)1..189
Gene:WAS
Gene Synonym:
Exon (2)190..330
Gene:WAS
Gene Synonym:
Exon (3)331..417
Gene:WAS
Gene Synonym:
Exon (4)418..520
Gene:WAS
Gene Synonym:
Exon (5)521..562
Gene:WAS
Gene Synonym:
Exon (6)563..616
Gene:WAS
Gene Synonym:
Exon (7)617..791
Gene:WAS
Gene Synonym:
Exon (8)792..834
Gene:WAS
Gene Synonym:
Exon (9)835..988
Gene:WAS
Gene Synonym:
Exon (10)989..1395
Gene:WAS
Gene Synonym:
Exon (11)1396..1510
Gene:WAS
Gene Synonym:
Exon (12)1511..1831
Gene:WAS
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000377
Organism Homo sapiens (human)
Definition Homo sapiens Wiskott-Aldrich syndrome (WAS), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000377

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGTGGGG GCCCAATGGG AGGAAGGCCC GGGGGCCGAG GAGCACCAGC GGTTCAGCAG 
AACATACCCT CCACCCTCCT CCAGGACCAC GAGAACCAGC GACTCTTTGA GATGCTTGGA
CGAAAATGCT TGACGCTGGC CACTGCAGTT GTTCAGCTGT ACCTGGCGCT GCCCCCTGGA
GCTGAGCACT GGACCAAGGA GCATTGTGGG GCTGTGTGCT TCGTGAAGGA TAACCCCCAG
AAGTCCTACT TCATCCGCCT TTACGGCCTT CAGGCTGGTC GGCTGCTCTG GGAACAGGAG
CTGTACTCAC AGCTTGTCTA CTCCACCCCC ACCCCCTTCT TCCACACCTT CGCTGGAGAT
GACTGCCAAG CGGGGCTGAA CTTTGCAGAC GAGGACGAGG CCCAGGCCTT CCGGGCCCTC
GTGCAGGAGA AGATACAAAA AAGGAATCAG AGGCAAAGTG GAGACAGACG CCAGCTACCC
CCACCACCAA CACCAGCCAA TGAAGAGAGA AGAGGAGGGC TCCCACCCCT GCCCCTGCAT
CCAGGTGGAG ACCAAGGAGG CCCTCCAGTG GGTCCGCTCT CCCTGGGGCT GGCGACAGTG
GACATCCAGA ACCCTGACAT CACGAGTTCA CGATACCGTG GGCTCCCAGC ACCTGGACCT
AGCCCAGCTG ATAAGAAACG CTCAGGGAAG AAGAAGATCA GCAAAGCTGA TATTGGTGCA
CCCAGTGGAT TCAAGCATGT CAGCCACGTG GGGTGGGACC CCCAGAATGG ATTTGACGTG
AACAACCTCG ACCCAGATCT GCGGAGTCTG TTCTCCAGGG CAGGAATCAG CGAGGCCCAG
CTCACCGACG CCGAGACCTC TAAACTTATC TACGACTTCA TTGAGGACCA GGGTGGGCTG
GAGGCTGTGC GGCAGGAGAT GAGGCGCCAG GAGCCACTTC CGCCGCCCCC ACCGCCATCT
CGAGGAGGGA ACCAGCTCCC CCGGCCCCCT ATTGTGGGGG GTAACAAGGG TCGTTCTGGT
CCACTGCCCC CTGTACCTTT GGGGATTGCC CCACCCCCAC CAACACCCCG GGGACCCCCA
CCCCCAGGCC GAGGGGGCCC TCCACCACCA CCCCCTCCAG CTACTGGACG TTCTGGACCA
CTGCCCCCTC CACCCCCTGG AGCTGGTGGG CCACCCATGC CACCACCACC GCCACCACCG
CCACCGCCGC CCAGCTCCGG GAATGGACCA GCCCCTCCCC CACTCCCTCC TGCTCTGGTG
CCTGCCGGGG GCCTGGCCCC TGGTGGGGGT CGGGGAGCGC TTTTGGATCA AATCCGGCAG
GGAATTCAGC TGAACAAGAC CCCTGGGGCC CCAGAGAGCT CAGCGCTGCA GCCACCACCT
CAGAGCTCAG AGGGACTGGT GGGGGCCCTG ATGCACGTGA TGCAGAAGAG AAGCAGAGCC
ATCCACTCCT CCGACGAAGG GGAGGACCAG GCTGGCGATG AAGATGAAGA TGATGAATGG
GATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
*
*
*
*