×

WIPF1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol WIPF1
Entrez Gene ID 7456
Full Name WAS/WASL interacting protein family member 1
Synonyms PRPL-2,WAS2,WASPIP,WIP
General protein information
Preferred Names

WAS/WASL interacting protein family member 1

Names

WAS/WASL-interacting protein family member 1
WASP-interacting protein
Wiskott-Aldrich syndrome protein interacting protein
protein PRPL-2
testicular tissue protein Li 226

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

2

2q31.1

Summary This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

602357


mRNA and Protein(s)

mRNA Protein Name
XM_011511780.2 XP_011510082.1 WAS/WASL-interacting protein family member 1 isoform X1
NM_001077269.1 NP_001070737.1 WAS/WASL-interacting protein family member 1
NM_003387.4 NP_003378.3 WAS/WASL-interacting protein family member 1



Gallus gallus (chicken) WIPF1 NP_001012935.1
Mus musculus (house mouse) Wipf1 NP_694778.1
Macaca mulatta (rhesus monkey) WIPF1 XP_001090880.1
Homo sapiens (human) WIPF1 NP_001070737.1
Rattus norvegicus (Norway rat) Wipf1 NP_476540.2
Canis lupus familiaris (dog) WIPF1 XP_545531.3
Pan troglodytes (chimpanzee) WIPF1 XP_001150625.1
Bos taurus (cattle) WIPF1 NP_001070391.1


Related articles in PubMed

Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages.
Vijayakumar V, Monypenny J, Chen XJ, Machesky LM, Lilla S, Thrasher AJ, Antón IM, Calle Y, Jones GE
Journal of cell science128(2)251-65(2015 Jan)

Triple-color FRET analysis reveals conformational changes in the WIP-WASp actin-regulating complex.
Fried S, Reicher B, Pauker MH, Eliyahu S, Matalon O, Noy E, Chill J, Barda-Saad M
Science signaling7(331)ra60(2014 Jun)

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
Blandin G, Marchand S, Charton K, Danièle N, Gicquel E, Boucheteil JB, Bentaib A, Barrault L, Stockholm D, Bartoli M, Richard I
Skeletal muscle3(1)3(2013 Feb)

WIP remodeling actin behind the scenes: how WIP reshapes immune and other functions.
Noy E, Fried S, Matalon O, Barda-Saad M
International journal of molecular sciences13(6)7629-47(2012)

A novel primary human immunodeficiency due to deficiency in the WASP-interacting protein WIP.
Lanzi G, Moratto D, Vairo D, Masneri S, Delmonte O, Paganini T, Parolini S, Tabellini G, Mazza C, Savoldi G, Montin D, Martino S, Tovo P, Pessach IM, Massaad MJ, Ramesh N, Porta F, Plebani A, Notarangelo LD, Geha RS, Giliani S
The Journal of experimental medicine209(1)29-34(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knocking down WIP expression in A549 cells significantly reduced RhoA levels and WIP was found to interact with RhoA suggesting that WIP might be executing its function by regulating RhoA.
Title: WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels.

Study provides evidence that WIP and WIRE contribute to breast cancer cell invasiveness through coordinated roles. WIP seems necessary for the assembly of invasive protrusions, whereas WIRE regulates their maturation, which leads to matrix degradation.
Title: WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion.

conclude that tyrosine phosphorylation of WIP is a crucial regulator of WASP stability and function as an actin-nucleation-promoting factor
Title: Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages.

Data indicate the WASp-interacting protein (WIP)-Wiskott-Aldrich syndrome protein (WASp) interaction in the regulation of actin-dependent processes.
Title: Triple-color FRET analysis reveals conformational changes in the WIP-WASp actin-regulating complex.

WIP was shown to interact with various binding partners, including the signaling proteins Nck, CrkL and cortactin.
Title: WIP remodeling actin behind the scenes: how WIP reshapes immune and other functions.

The following WIPF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WIPF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu03312 NM_003387.4
Latest version!
Homo sapiens WAS/WASL interacting protein family member 1 (WIPF1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $300.30
$429.00
OHu03312 NM_001077269.1
Latest version!
Homo sapiens WAS/WASL interacting protein family member 1 (WIPF1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $300.30
$429.00
OHu03312 XM_011511780.2
Latest version!
Homo sapiens WAS/WASL interacting protein family member 1 (WIPF1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $300.30
$429.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu03312
Clone ID Related Accession (Same CDS sequence) XM_011511780.2 , NM_001077269.1 , NM_003387.4
Accession Version NM_001077269.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-25
Organism Homo sapiens(Human)
Product WAS/WASL-interacting protein family member 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640870.1, BC110288.1, AL529597.3, BC002914.2, AL832275.1, BM475558.1, AA903457.1 and BC045584.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005539.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCTGTCC CTCCCCCTCC AGCACCCCCG CCGCCCCCGA CGTTTGCACT GGCCAATACA 
GAGAAGCCTA CCTTGAATAA GACAGAGCAG GCTGGGAGAA ATGCTCTCCT TTCTGATATC
AGCAAAGGGA AGAAACTAAA GAAGACGGTC ACCAATGACA GAAGTGCACC AATACTGGAC
AAACCTAAAG GAGCTGGTGC TGGAGGCGGT GGTGGTGGCT TTGGTGGAGG CGGCGGATTT
GGCGGAGGAG GTGGTGGCGG AGGCGGTGGA AGTTTTGGAG GGGGCGGACC TCCAGGTCTG
GGAGGATTGT TCCAGGCTGG AATGCCGAAG CTGAGATCCA CGGCCAACAG GGATAATGAT
TCTGGAGGAA GCCGACCACC ATTGTTGCCA CCGGGAGGAA GATCCACATC TGCGAAACCC
TTTTCACCCC CAAGTGGCCC AGGGAGGTTT CCTGTGCCTT CTCCAGGCCA CAGAAGTGGT
CCCCCAGAGC CTCAGAGGAA CCGAATGCCG CCCCCAAGGC CCGACGTGGG CTCAAAGCCT
GATAGCATTC CTCCTCCAGT ACCTAGTACT CCAAGACCCA TTCAATCAAG TCCGCACAAC
CGGGGGTCCC CACCAGTGCC CGGAGGCCCC AGGCAGCCCA GCCCCGGGCC CACTCCTCCC
CCTTTCCCTG GAAACCGCGG CACTGCTTTG GGAGGAGGCT CAATACGTCA GTCCCCCTTG
AGCTCCTCCT CGCCCTTCTC CAACCGGCCT CCCCTGCCGC CTACCCCCAG CAGGGCCTTG
GATGACAAAC CCCCTCCACC ACCTCCTCCA GTGGGCAACA GGCCCTCCAT CCACAGGGAA
GCGGTTCCCC CTCCTCCTCC TCAGAACAAC AAGCCTCCAG TGCCTTCCAC TCCGCGGCCT
TCGGCCTCCT CACAGGCCCC ACCTCCGCCG CCACCTCCCA GCAGGCCCGG GCCGCCTCCT
CTGCCTCCAA GTTCCAGCGG CAATGACGAA ACCCCAAGAC TCCCACAGCG GAATCTGTCC
CTCAGTTCGT CCACGCCCCC GTTACCTTCG CCAGGACGTT CAGGTCCTCT TCCTCCCCCG
CCCAGTGAGA GACCCCCACC TCCAGTGAGG GACCCGCCAG GCCGATCAGG CCCCCTCCCA
CCACCTCCTC CAGTAAGCAG AAACGGCAGC ACATCTCGGG CCCTGCCTGC TACCCCTCAG
TTGCCATCCA GGAGTGGAGT AGACAGTCCC AGGAGTGGAC CCAGGCCTCC CCTTCCTCCT
GATAGGCCCA GTGCTGGGGC ACCTCCCCCA CCTCCACCAT CAACATCTAT TAGAAATGGC
TTCCAAGACT CTCCATGTGA AGATGAGTGG GAAAGCAGAT TCTACTTCCA TCCGATTTCC
GATTTGCCAC CTCCAGAGCC ATATGTACAA ACGACCAAAA GTTATCCCAG CAAACTGGCA
AGAAACGAAA GCCGGAGTGG ATCCAACCGA AGAGAAAGGG GTGCTCCACC ACTCCCTCCC
ATCCCGAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001070737.1
CDS165..1676
Misc Feature(1)153..155
Misc Feature(2)255..329
Misc Feature(3)297..308
Misc Feature(4)588..590
Misc Feature(5)864..866
Misc Feature(6)1182..1184
Misc Feature(7)1197..1199
Misc Feature(8)1212..1214
Misc Feature(9)1218..1247
Misc Feature(10)1284..1313
Misc Feature(11)1392..1421
Exon (1)1..126
Exon (2)127..215
Exon (3)216..345
Exon (4)346..522
Exon (5)523..1293
Exon (6)1294..1506
Exon (7)1507..1620
Exon (8)1621..4649
Translation

Target ORF information:

RefSeq Version NM_001077269.1
Organism Homo sapiens(Human)
Definition Homo sapiens WAS/WASL interacting protein family member 1 (WIPF1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001077269.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCTGTCC CTCCCCCTCC AGCACCCCCG CCGCCCCCGA CGTTTGCACT GGCCAATACA 
GAGAAGCCTA CCTTGAATAA GACAGAGCAG GCTGGGAGAA ATGCTCTCCT TTCTGATATC
AGCAAAGGGA AGAAACTAAA GAAGACGGTC ACCAATGACA GAAGTGCACC AATACTGGAC
AAACCTAAAG GAGCTGGTGC TGGAGGCGGT GGTGGTGGCT TTGGTGGAGG CGGCGGATTT
GGCGGAGGAG GTGGTGGCGG AGGCGGTGGA AGTTTTGGAG GGGGCGGACC TCCAGGTCTG
GGAGGATTGT TCCAGGCTGG AATGCCGAAG CTGAGATCCA CGGCCAACAG GGATAATGAT
TCTGGAGGAA GCCGACCACC ATTGTTGCCA CCGGGAGGAA GATCCACATC TGCGAAACCC
TTTTCACCCC CAAGTGGCCC AGGGAGGTTT CCTGTGCCTT CTCCAGGCCA CAGAAGTGGT
CCCCCAGAGC CTCAGAGGAA CCGAATGCCG CCCCCAAGGC CCGACGTGGG CTCAAAGCCT
GATAGCATTC CTCCTCCAGT ACCTAGTACT CCAAGACCCA TTCAATCAAG TCCGCACAAC
CGGGGGTCCC CACCAGTGCC CGGAGGCCCC AGGCAGCCCA GCCCCGGGCC CACTCCTCCC
CCTTTCCCTG GAAACCGCGG CACTGCTTTG GGAGGAGGCT CAATACGTCA GTCCCCCTTG
AGCTCCTCCT CGCCCTTCTC CAACCGGCCT CCCCTGCCGC CTACCCCCAG CAGGGCCTTG
GATGACAAAC CCCCTCCACC ACCTCCTCCA GTGGGCAACA GGCCCTCCAT CCACAGGGAA
GCGGTTCCCC CTCCTCCTCC TCAGAACAAC AAGCCTCCAG TGCCTTCCAC TCCGCGGCCT
TCGGCCTCCT CACAGGCCCC ACCTCCGCCG CCACCTCCCA GCAGGCCCGG GCCGCCTCCT
CTGCCTCCAA GTTCCAGCGG CAATGACGAA ACCCCAAGAC TCCCACAGCG GAATCTGTCC
CTCAGTTCGT CCACGCCCCC GTTACCTTCG CCAGGACGTT CAGGTCCTCT TCCTCCCCCG
CCCAGTGAGA GACCCCCACC TCCAGTGAGG GACCCGCCAG GCCGATCAGG CCCCCTCCCA
CCACCTCCTC CAGTAAGCAG AAACGGCAGC ACATCTCGGG CCCTGCCTGC TACCCCTCAG
TTGCCATCCA GGAGTGGAGT AGACAGTCCC AGGAGTGGAC CCAGGCCTCC CCTTCCTCCT
GATAGGCCCA GTGCTGGGGC ACCTCCCCCA CCTCCACCAT CAACATCTAT TAGAAATGGC
TTCCAAGACT CTCCATGTGA AGATGAGTGG GAAAGCAGAT TCTACTTCCA TCCGATTTCC
GATTTGCCAC CTCCAGAGCC ATATGTACAA ACGACCAAAA GTTATCCCAG CAAACTGGCA
AGAAACGAAA GCCGGAGTGG ATCCAACCGA AGAGAAAGGG GTGCTCCACC ACTCCCTCCC
ATCCCGAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03312
Clone ID Related Accession (Same CDS sequence) XM_011511780.2 , NM_001077269.1 , NM_003387.4
Accession Version NM_003387.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-25
Organism Homo sapiens(Human)
Product WAS/WASL-interacting protein family member 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA674627.1, BX640870.1, BC110288.1, AL529597.3, BC002914.2, AL832275.1, BM475558.1, AA903457.1 and BC045584.1. On Oct 19, 2006 this sequence version replaced NM_003387.3. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF031588.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCTGTCC CTCCCCCTCC AGCACCCCCG CCGCCCCCGA CGTTTGCACT GGCCAATACA 
GAGAAGCCTA CCTTGAATAA GACAGAGCAG GCTGGGAGAA ATGCTCTCCT TTCTGATATC
AGCAAAGGGA AGAAACTAAA GAAGACGGTC ACCAATGACA GAAGTGCACC AATACTGGAC
AAACCTAAAG GAGCTGGTGC TGGAGGCGGT GGTGGTGGCT TTGGTGGAGG CGGCGGATTT
GGCGGAGGAG GTGGTGGCGG AGGCGGTGGA AGTTTTGGAG GGGGCGGACC TCCAGGTCTG
GGAGGATTGT TCCAGGCTGG AATGCCGAAG CTGAGATCCA CGGCCAACAG GGATAATGAT
TCTGGAGGAA GCCGACCACC ATTGTTGCCA CCGGGAGGAA GATCCACATC TGCGAAACCC
TTTTCACCCC CAAGTGGCCC AGGGAGGTTT CCTGTGCCTT CTCCAGGCCA CAGAAGTGGT
CCCCCAGAGC CTCAGAGGAA CCGAATGCCG CCCCCAAGGC CCGACGTGGG CTCAAAGCCT
GATAGCATTC CTCCTCCAGT ACCTAGTACT CCAAGACCCA TTCAATCAAG TCCGCACAAC
CGGGGGTCCC CACCAGTGCC CGGAGGCCCC AGGCAGCCCA GCCCCGGGCC CACTCCTCCC
CCTTTCCCTG GAAACCGCGG CACTGCTTTG GGAGGAGGCT CAATACGTCA GTCCCCCTTG
AGCTCCTCCT CGCCCTTCTC CAACCGGCCT CCCCTGCCGC CTACCCCCAG CAGGGCCTTG
GATGACAAAC CCCCTCCACC ACCTCCTCCA GTGGGCAACA GGCCCTCCAT CCACAGGGAA
GCGGTTCCCC CTCCTCCTCC TCAGAACAAC AAGCCTCCAG TGCCTTCCAC TCCGCGGCCT
TCGGCCTCCT CACAGGCCCC ACCTCCGCCG CCACCTCCCA GCAGGCCCGG GCCGCCTCCT
CTGCCTCCAA GTTCCAGCGG CAATGACGAA ACCCCAAGAC TCCCACAGCG GAATCTGTCC
CTCAGTTCGT CCACGCCCCC GTTACCTTCG CCAGGACGTT CAGGTCCTCT TCCTCCCCCG
CCCAGTGAGA GACCCCCACC TCCAGTGAGG GACCCGCCAG GCCGATCAGG CCCCCTCCCA
CCACCTCCTC CAGTAAGCAG AAACGGCAGC ACATCTCGGG CCCTGCCTGC TACCCCTCAG
TTGCCATCCA GGAGTGGAGT AGACAGTCCC AGGAGTGGAC CCAGGCCTCC CCTTCCTCCT
GATAGGCCCA GTGCTGGGGC ACCTCCCCCA CCTCCACCAT CAACATCTAT TAGAAATGGC
TTCCAAGACT CTCCATGTGA AGATGAGTGG GAAAGCAGAT TCTACTTCCA TCCGATTTCC
GATTTGCCAC CTCCAGAGCC ATATGTACAA ACGACCAAAA GTTATCCCAG CAAACTGGCA
AGAAACGAAA GCCGGAGTGG ATCCAACCGA AGAGAAAGGG GTGCTCCACC ACTCCCTCCC
ATCCCGAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003378.3
CDS101..1612
Misc Feature(1)89..91
Misc Feature(2)191..265
Misc Feature(3)233..244
Misc Feature(4)524..526
Misc Feature(5)800..802
Misc Feature(6)1118..1120
Misc Feature(7)1133..1135
Misc Feature(8)1148..1150
Misc Feature(9)1154..1183
Misc Feature(10)1220..1249
Misc Feature(11)1328..1357
Misc Feature(12)1562..1564
Exon (1)1..62
Exon (2)63..151
Exon (3)152..281
Exon (4)282..458
Exon (5)459..1229
Exon (6)1230..1442
Exon (7)1443..1556
Exon (8)1557..4585
Translation

Target ORF information:

RefSeq Version NM_003387.4
Organism Homo sapiens(Human)
Definition Homo sapiens WAS/WASL interacting protein family member 1 (WIPF1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003387.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCTGTCC CTCCCCCTCC AGCACCCCCG CCGCCCCCGA CGTTTGCACT GGCCAATACA 
GAGAAGCCTA CCTTGAATAA GACAGAGCAG GCTGGGAGAA ATGCTCTCCT TTCTGATATC
AGCAAAGGGA AGAAACTAAA GAAGACGGTC ACCAATGACA GAAGTGCACC AATACTGGAC
AAACCTAAAG GAGCTGGTGC TGGAGGCGGT GGTGGTGGCT TTGGTGGAGG CGGCGGATTT
GGCGGAGGAG GTGGTGGCGG AGGCGGTGGA AGTTTTGGAG GGGGCGGACC TCCAGGTCTG
GGAGGATTGT TCCAGGCTGG AATGCCGAAG CTGAGATCCA CGGCCAACAG GGATAATGAT
TCTGGAGGAA GCCGACCACC ATTGTTGCCA CCGGGAGGAA GATCCACATC TGCGAAACCC
TTTTCACCCC CAAGTGGCCC AGGGAGGTTT CCTGTGCCTT CTCCAGGCCA CAGAAGTGGT
CCCCCAGAGC CTCAGAGGAA CCGAATGCCG CCCCCAAGGC CCGACGTGGG CTCAAAGCCT
GATAGCATTC CTCCTCCAGT ACCTAGTACT CCAAGACCCA TTCAATCAAG TCCGCACAAC
CGGGGGTCCC CACCAGTGCC CGGAGGCCCC AGGCAGCCCA GCCCCGGGCC CACTCCTCCC
CCTTTCCCTG GAAACCGCGG CACTGCTTTG GGAGGAGGCT CAATACGTCA GTCCCCCTTG
AGCTCCTCCT CGCCCTTCTC CAACCGGCCT CCCCTGCCGC CTACCCCCAG CAGGGCCTTG
GATGACAAAC CCCCTCCACC ACCTCCTCCA GTGGGCAACA GGCCCTCCAT CCACAGGGAA
GCGGTTCCCC CTCCTCCTCC TCAGAACAAC AAGCCTCCAG TGCCTTCCAC TCCGCGGCCT
TCGGCCTCCT CACAGGCCCC ACCTCCGCCG CCACCTCCCA GCAGGCCCGG GCCGCCTCCT
CTGCCTCCAA GTTCCAGCGG CAATGACGAA ACCCCAAGAC TCCCACAGCG GAATCTGTCC
CTCAGTTCGT CCACGCCCCC GTTACCTTCG CCAGGACGTT CAGGTCCTCT TCCTCCCCCG
CCCAGTGAGA GACCCCCACC TCCAGTGAGG GACCCGCCAG GCCGATCAGG CCCCCTCCCA
CCACCTCCTC CAGTAAGCAG AAACGGCAGC ACATCTCGGG CCCTGCCTGC TACCCCTCAG
TTGCCATCCA GGAGTGGAGT AGACAGTCCC AGGAGTGGAC CCAGGCCTCC CCTTCCTCCT
GATAGGCCCA GTGCTGGGGC ACCTCCCCCA CCTCCACCAT CAACATCTAT TAGAAATGGC
TTCCAAGACT CTCCATGTGA AGATGAGTGG GAAAGCAGAT TCTACTTCCA TCCGATTTCC
GATTTGCCAC CTCCAGAGCC ATATGTACAA ACGACCAAAA GTTATCCCAG CAAACTGGCA
AGAAACGAAA GCCGGAGTGG ATCCAACCGA AGAGAAAGGG GTGCTCCACC ACTCCCTCCC
ATCCCGAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03312
Clone ID Related Accession (Same CDS sequence) XM_011511780.2 , NM_001077269.1 , NM_003387.4
Accession Version XM_011511780.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1512bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product WAS/WASL-interacting protein family member 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCTGTCC CTCCCCCTCC AGCACCCCCG CCGCCCCCGA CGTTTGCACT GGCCAATACA 
GAGAAGCCTA CCTTGAATAA GACAGAGCAG GCTGGGAGAA ATGCTCTCCT TTCTGATATC
AGCAAAGGGA AGAAACTAAA GAAGACGGTC ACCAATGACA GAAGTGCACC AATACTGGAC
AAACCTAAAG GAGCTGGTGC TGGAGGCGGT GGTGGTGGCT TTGGTGGAGG CGGCGGATTT
GGCGGAGGAG GTGGTGGCGG AGGCGGTGGA AGTTTTGGAG GGGGCGGACC TCCAGGTCTG
GGAGGATTGT TCCAGGCTGG AATGCCGAAG CTGAGATCCA CGGCCAACAG GGATAATGAT
TCTGGAGGAA GCCGACCACC ATTGTTGCCA CCGGGAGGAA GATCCACATC TGCGAAACCC
TTTTCACCCC CAAGTGGCCC AGGGAGGTTT CCTGTGCCTT CTCCAGGCCA CAGAAGTGGT
CCCCCAGAGC CTCAGAGGAA CCGAATGCCG CCCCCAAGGC CCGACGTGGG CTCAAAGCCT
GATAGCATTC CTCCTCCAGT ACCTAGTACT CCAAGACCCA TTCAATCAAG TCCGCACAAC
CGGGGGTCCC CACCAGTGCC CGGAGGCCCC AGGCAGCCCA GCCCCGGGCC CACTCCTCCC
CCTTTCCCTG GAAACCGCGG CACTGCTTTG GGAGGAGGCT CAATACGTCA GTCCCCCTTG
AGCTCCTCCT CGCCCTTCTC CAACCGGCCT CCCCTGCCGC CTACCCCCAG CAGGGCCTTG
GATGACAAAC CCCCTCCACC ACCTCCTCCA GTGGGCAACA GGCCCTCCAT CCACAGGGAA
GCGGTTCCCC CTCCTCCTCC TCAGAACAAC AAGCCTCCAG TGCCTTCCAC TCCGCGGCCT
TCGGCCTCCT CACAGGCCCC ACCTCCGCCG CCACCTCCCA GCAGGCCCGG GCCGCCTCCT
CTGCCTCCAA GTTCCAGCGG CAATGACGAA ACCCCAAGAC TCCCACAGCG GAATCTGTCC
CTCAGTTCGT CCACGCCCCC GTTACCTTCG CCAGGACGTT CAGGTCCTCT TCCTCCCCCG
CCCAGTGAGA GACCCCCACC TCCAGTGAGG GACCCGCCAG GCCGATCAGG CCCCCTCCCA
CCACCTCCTC CAGTAAGCAG AAACGGCAGC ACATCTCGGG CCCTGCCTGC TACCCCTCAG
TTGCCATCCA GGAGTGGAGT AGACAGTCCC AGGAGTGGAC CCAGGCCTCC CCTTCCTCCT
GATAGGCCCA GTGCTGGGGC ACCTCCCCCA CCTCCACCAT CAACATCTAT TAGAAATGGC
TTCCAAGACT CTCCATGTGA AGATGAGTGG GAAAGCAGAT TCTACTTCCA TCCGATTTCC
GATTTGCCAC CTCCAGAGCC ATATGTACAA ACGACCAAAA GTTATCCCAG CAAACTGGCA
AGAAACGAAA GCCGGAGTGG ATCCAACCGA AGAGAAAGGG GTGCTCCACC ACTCCCTCCC
ATCCCGAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510082.1
CDS287..1798
Misc Feature(1)377..451
Translation

Target ORF information:

RefSeq Version XM_011511780.2
Organism Homo sapiens(Human)
Definition Homo sapiens WAS/WASL interacting protein family member 1 (WIPF1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011511780.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCTGTCC CTCCCCCTCC AGCACCCCCG CCGCCCCCGA CGTTTGCACT GGCCAATACA 
GAGAAGCCTA CCTTGAATAA GACAGAGCAG GCTGGGAGAA ATGCTCTCCT TTCTGATATC
AGCAAAGGGA AGAAACTAAA GAAGACGGTC ACCAATGACA GAAGTGCACC AATACTGGAC
AAACCTAAAG GAGCTGGTGC TGGAGGCGGT GGTGGTGGCT TTGGTGGAGG CGGCGGATTT
GGCGGAGGAG GTGGTGGCGG AGGCGGTGGA AGTTTTGGAG GGGGCGGACC TCCAGGTCTG
GGAGGATTGT TCCAGGCTGG AATGCCGAAG CTGAGATCCA CGGCCAACAG GGATAATGAT
TCTGGAGGAA GCCGACCACC ATTGTTGCCA CCGGGAGGAA GATCCACATC TGCGAAACCC
TTTTCACCCC CAAGTGGCCC AGGGAGGTTT CCTGTGCCTT CTCCAGGCCA CAGAAGTGGT
CCCCCAGAGC CTCAGAGGAA CCGAATGCCG CCCCCAAGGC CCGACGTGGG CTCAAAGCCT
GATAGCATTC CTCCTCCAGT ACCTAGTACT CCAAGACCCA TTCAATCAAG TCCGCACAAC
CGGGGGTCCC CACCAGTGCC CGGAGGCCCC AGGCAGCCCA GCCCCGGGCC CACTCCTCCC
CCTTTCCCTG GAAACCGCGG CACTGCTTTG GGAGGAGGCT CAATACGTCA GTCCCCCTTG
AGCTCCTCCT CGCCCTTCTC CAACCGGCCT CCCCTGCCGC CTACCCCCAG CAGGGCCTTG
GATGACAAAC CCCCTCCACC ACCTCCTCCA GTGGGCAACA GGCCCTCCAT CCACAGGGAA
GCGGTTCCCC CTCCTCCTCC TCAGAACAAC AAGCCTCCAG TGCCTTCCAC TCCGCGGCCT
TCGGCCTCCT CACAGGCCCC ACCTCCGCCG CCACCTCCCA GCAGGCCCGG GCCGCCTCCT
CTGCCTCCAA GTTCCAGCGG CAATGACGAA ACCCCAAGAC TCCCACAGCG GAATCTGTCC
CTCAGTTCGT CCACGCCCCC GTTACCTTCG CCAGGACGTT CAGGTCCTCT TCCTCCCCCG
CCCAGTGAGA GACCCCCACC TCCAGTGAGG GACCCGCCAG GCCGATCAGG CCCCCTCCCA
CCACCTCCTC CAGTAAGCAG AAACGGCAGC ACATCTCGGG CCCTGCCTGC TACCCCTCAG
TTGCCATCCA GGAGTGGAGT AGACAGTCCC AGGAGTGGAC CCAGGCCTCC CCTTCCTCCT
GATAGGCCCA GTGCTGGGGC ACCTCCCCCA CCTCCACCAT CAACATCTAT TAGAAATGGC
TTCCAAGACT CTCCATGTGA AGATGAGTGG GAAAGCAGAT TCTACTTCCA TCCGATTTCC
GATTTGCCAC CTCCAGAGCC ATATGTACAA ACGACCAAAA GTTATCCCAG CAAACTGGCA
AGAAACGAAA GCCGGAGTGG ATCCAACCGA AGAGAAAGGG GTGCTCCACC ACTCCCTCCC
ATCCCGAGGT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages.
Journal of cell science128(2)251-65(2015 Jan)
Vijayakumar V,Monypenny J,Chen XJ,Machesky LM,Lilla S,Thrasher AJ,Antón IM,Calle Y,Jones GE


book

Triple-color FRET analysis reveals conformational changes in the WIP-WASp actin-regulating complex.
Science signaling7(331)ra60(2014 Jun)
Fried S,Reicher B,Pauker MH,Eliyahu S,Matalon O,Noy E,Chill J,Barda-Saad M


book

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.
Skeletal muscle3(1)3(2013 Feb)
Blandin G,Marchand S,Charton K,Danièle N,Gicquel E,Boucheteil JB,Bentaib A,Barrault L,Stockholm D,Bartoli M,Richard I


book

WIP remodeling actin behind the scenes: how WIP reshapes immune and other functions.
International journal of molecular sciences13(6)7629-47(2012)
Noy E,Fried S,Matalon O,Barda-Saad M


book

A novel primary human immunodeficiency due to deficiency in the WASP-interacting protein WIP.
The Journal of experimental medicine209(1)29-34(2012 Jan)
Lanzi G,Moratto D,Vairo D,Masneri S,Delmonte O,Paganini T,Parolini S,Tabellini G,Mazza C,Savoldi G,Montin D,Martino S,Tovo P,Pessach IM,Massaad MJ,Ramesh N,Porta F,Plebani A,Notarangelo LD,Geha RS,Giliani S


 
*
*
*
*