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WRN cDNA ORF clone, Homo sapiens (human)

Gene Symbol WRN
Entrez Gene ID 7486
Full Name Werner syndrome, RecQ helicase-like
Synonyms RECQ3, RECQL2, RECQL3
General protein information
Preferred Names
Werner syndrome ATP-dependent helicase
Names
Werner syndrome ATP-dependent helicase
exonuclease WRN
recQ protein-like 2
DNA helicase, RecQ-like type 3
NP_000544.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

8

8p12

Summary This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604611

Disorder Html: Werner syndrome, 277700 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011544639 XP_011542941 Werner syndrome ATP-dependent helicase isoform X1
XM_011544640 XP_011542942 Werner syndrome ATP-dependent helicase isoform X2
NM_000553 NP_000544 Werner syndrome ATP-dependent helicase



Homo sapiens (human) WRN NP_000544.2
Pan troglodytes (chimpanzee) WRN XP_528104.2
Macaca mulatta (Rhesus monkey) WRN XP_001085031.1
Canis lupus familiaris (dog) WRN XP_539984.4
Bos taurus (cattle) WRN NP_001137329.2
Mus musculus (house mouse) Wrn NP_035851.3
Rattus norvegicus (Norway rat) RGD1564788 XP_006253317.1
Gallus gallus (chicken) WRN XP_001235033.2
Danio rerio (zebrafish) wrn XP_697980.5
Caenorhabditis elegans wrn-1 NP_495324.2
Xenopus (Silurana) tropicalis (western clawed frog) wrn XP_002939304.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following WRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu61339 XM_011544639 PREDICTED: Homo sapiens Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu61340 XM_011544640 PREDICTED: Homo sapiens Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu18809 NM_000553 Homo sapiens Werner syndrome, RecQ helicase-like (WRN), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $727.30
$1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu61339
Clone ID Related Accession (Same CDS sequence) XM_011544639
Accession Version XM_011544639.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Werner syndrome ATP-dependent helicase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTCGGAA GAGTGTTTTT
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA CGATGCTAGT
GATTGCTCTT TCCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACG TTTCTTCCAT GTCAGTTTTT
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGCACAGAGA CCTGGAGCCT TAACAGTCTG
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AATTGGAAAA CCCACGGAGG
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT
GAAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT
CACGTTGAAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA
GATGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT
GAACTCCAAA TTTTGGAACA GCAGTCTCAG GAAGAATATC TTAGTGATAT TGCTTATAAA
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTCAATAG TGGCACGGTA
GAACCAACTC ATTCTAAATG CTTAAAAATG GAAAGAAATC TGGGTCTTCC TACTAAAGAA
GAAGAAGAAG ATGATGAAAA TGAAGCTAAT GAAGGGGAAG AAGATGATGA TAAGGACTTT
TTGTGGCCAG CACCCAATGA AGAGCAAGTT ACTTGCCTCA AGATGTACTT TGGCCATTCC
AGTTTTAAAC CAGTTCAGTG GAAAGTGATT CATTCAGTAT TAGAAGAAAG AAGAGATAAT
GTTGCTGTCA TGGCAACTGG ATATGGAAAG AGTTTGTGCT TCCAGTATCC ACCTGTTTAT
GTAGGCAAGA TTGGCCTTGT TATCTCTCCC CTTATTTCTC TGATGGAAGA CCAAGTGCTA
CAGCTTAAAA TGTCCAACAT CCCAGCTTGC TTCCTTGGAT CAGCACAGTC AGAAAATGTT
CTAACAGATA TTAAATTAGG TAAATACCGG ATTGTATACG TAACTCCAGA ATACTGTTCA
GGTAACATGG GCCTGCTCCA GCAACTTGAG GCTGATATTG GTATCACGCT CATTGCTGTG
GATGAGGCTC ACTGTATTTC TGAGTGGGGG CATGATTTTA GGGATTCATT CAGGAAGTTG
GGCTCCCTAA AGACAGCACT GCCAATGGTT CCAATCGTTG CACTTACTGC TACTGCAAGT
TCTTCAATCC GGGAAGACAT TGTACGTTGC TTAAATCTGA GAAATCCTCA GATCACCTGT
ACTGGTTTTG ATCGACCAAA CCTGTATTTA GAAGTTAGGC GAAAAACAGG GAATATCCTT
CAGGATCTGC AGCCATTTCT TGTCAAAACA AGTTCCCACT GGGAATTTGA AGGTCCAACA
ATCATCTACT GTCCTTCTAG AAAAATGACA CAACAAGTTA CAGGTGAACT TAGGAAACTG
AATCTATCCT GTGGAACATA CCATGCGGGC ATGAGTTTTA GCACAAGGAA AGACATTCAT
CATAGGTTTG TAAGAGATGA AATTCAGTGT GTCATAGCTA CCATAGCTTT TGGAATGGGC
ATTAATAAAG CTGACATTCG CCAAGTCATT CATTACGGTG CTCCTAAGGA CATGGAATCA
TATTATCAGG AGATTGGTAG AGCTGGTCGT GATGGACTTC AAAGTTCTTG TCACGTCCTC
TGGGCTCCTG CAGACATTAA CTTAAATAGG CACCTTCTTA CTGAGATACG TAATGAGAAG
TTTCGATTAT ACAAATTAAA GATGATGGCA AAGATGGAAA AATATCTTCA TTCTAGCAGA
TGTAGGAGAC AAATCATCTT GTCTCATTTT GAGGACAAAC AAGTACAAAA AGCCTCCTTG
GGAATTATGG GAACTGAAAA ATGCTGTGAT AATTGCAGGT CCAGATTGGA TCATTGCTAT
TCCATGGATG ACTCAGAGGA TACATCCTGG GACTTTGGTC CACAAGCATT TAAGCTTTTG
TCTGCTGTGG ACATCTTAGG CGAAAAATTT GGAATTGGGC TTCCAATTTT ATTTCTCCGA
GGATCTAATT CTCAGCGTCT TGCCGATCAA TATCGCAGGC ACAGTTTATT TGGCACTGGC
AAGGATCAAA CAGAGAGTTG GTGGAAGGCT TTTTCCCGTC AGCTGATCAC TGAGGGATTC
TTGGTAGAAG TTTCTCGGTA TAACAAATTT ATGAAGATTT GCGCCCTTAC GAAAAAGGGT
AGAAATTGGC TTCATAAAGC TAATACAGAA TCTCAGAGCC TCATCCTTCA AGCTAATGAA
GAATTGTGTC CAAAGAAGTT GCTTCTGCCT AGTTCGAAAA CTGTATCTTC GGGCACCAAA
GAGCATTGTT ATAATCAAGT ACCAGTTGAA TTAAGTACAG AGAAGAAGTC TAACTTGGAG
AAGTTATATT CTTATAAACC ATGTGATAAG ATTTCTTCTG GGAGTAACAT TTCTAAAAAA
AGTATCATGG TACAGTCACC AGAAAAAGCT TACAGTTCCT CACAGCCTGT TATTTCGGCA
CAAGAGCAGG AGACTCAGAT TGTGTTATAT GGCAAATTGG TAGAAGCTAG GCAGAAACAT
GCCAATAAAA TGGATGTTCC CCCAGCTATT CTGGCAACAA ACAAGATACT GGTGGATATG
GCCAAAATGA GACCAACTAC GGTTGAAAAC GTAAAAAGGA TTGATGGTGT TTCTGAAGGC
AAAGCTGCCA TGTTGGCCCC TCTGTTGGAA GTCATCAAAC ATTTCTGCCA AACAAATAGT
GTTCAGACAG ACCTCTTTTC AAGTACAAAA CCTCAAGAAG AACAGAAGAC GAGTCTGGTA
GCAAAAAATA AAATATGCAC ACTTTCACAG TCTATGGCCA TCACATACTC TTTATTCCAA
GAAAAGAAGA TGCCTTTGAA GAGCATAGCT GAGAGCAGGA TTCTGCCTCT CATGACAATT
GGCATGCACT TATCCCAAGC GGTGAAAGCT GGCTGCCCCC TTGATTTGGA GCGAGCAGGC
CTGACTCCAG AGGTTCAGAA GATTATTGCT GATGTTATCC GAAACCCTCC CGTCAACTCA
GATATGAGTA AAATTAGCCT AATCAGAATG TTAGTTCCTG AAAACATTGA CACGTACCTT
ATCCACATGG CAATTGAGAT CCTTAAACAT GGTCCTGACA GCGGACTTCA ACCTTCATGT
GATGTCAACA AAAGGAGATG TTTTCCCGGT TCTGAAGAGA TCTGTTCAAG TTCTAAGAGA
AGCAAGGAAG AAGTAGGCAT CAATACTGAG ACTTCATCTG CAGAGAGAAA GAGACGATTA
CCTGTGTGGT TTGCCAAAGG AAGTGATACC AGCAAGAAAT TAATGGACAA AACGAAAAGG
GGAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542941.1
CDS273..4490
Misc Feature(1)453..959(+)
Misc Feature(2)516..920(+)
Misc Feature(3)516..920(+)
Misc Feature(4)519..920(+)
Misc Feature(5)1803..3221(+)
Misc Feature(6)1884..2306(+)
Misc Feature(7)1911..1925(+)
Misc Feature(8)2193..2204(+)
Misc Feature(9)2466..2792(+)
Misc Feature(10)2505..2660(+)
Misc Feature(11)2676..2762(+)
Misc Feature(12)3063..3344(+)
Misc Feature(13)3657..3878(+)
Misc Feature(14)3960..4247(+)
Translation

Target ORF information:

RefSeq Version XM_011544639
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544639

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTCGGAA GAGTGTTTTT
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA CGATGCTAGT
GATTGCTCTT TCCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACG TTTCTTCCAT GTCAGTTTTT
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGCACAGAGA CCTGGAGCCT TAACAGTCTG
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AATTGGAAAA CCCACGGAGG
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT
GAAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT
CACGTTGAAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA
GATGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT
GAACTCCAAA TTTTGGAACA GCAGTCTCAG GAAGAATATC TTAGTGATAT TGCTTATAAA
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT
GATGAAGATT TAGAAATGGA GATGCTTAAG TCTTTAGAAA ACCTCAATAG TGGCACGGTA
GAACCAACTC ATTCTAAATG CTTAAAAATG GAAAGAAATC TGGGTCTTCC TACTAAAGAA
GAAGAAGAAG ATGATGAAAA TGAAGCTAAT GAAGGGGAAG AAGATGATGA TAAGGACTTT
TTGTGGCCAG CACCCAATGA AGAGCAAGTT ACTTGCCTCA AGATGTACTT TGGCCATTCC
AGTTTTAAAC CAGTTCAGTG GAAAGTGATT CATTCAGTAT TAGAAGAAAG AAGAGATAAT
GTTGCTGTCA TGGCAACTGG ATATGGAAAG AGTTTGTGCT TCCAGTATCC ACCTGTTTAT
GTAGGCAAGA TTGGCCTTGT TATCTCTCCC CTTATTTCTC TGATGGAAGA CCAAGTGCTA
CAGCTTAAAA TGTCCAACAT CCCAGCTTGC TTCCTTGGAT CAGCACAGTC AGAAAATGTT
CTAACAGATA TTAAATTAGG TAAATACCGG ATTGTATACG TAACTCCAGA ATACTGTTCA
GGTAACATGG GCCTGCTCCA GCAACTTGAG GCTGATATTG GTATCACGCT CATTGCTGTG
GATGAGGCTC ACTGTATTTC TGAGTGGGGG CATGATTTTA GGGATTCATT CAGGAAGTTG
GGCTCCCTAA AGACAGCACT GCCAATGGTT CCAATCGTTG CACTTACTGC TACTGCAAGT
TCTTCAATCC GGGAAGACAT TGTACGTTGC TTAAATCTGA GAAATCCTCA GATCACCTGT
ACTGGTTTTG ATCGACCAAA CCTGTATTTA GAAGTTAGGC GAAAAACAGG GAATATCCTT
CAGGATCTGC AGCCATTTCT TGTCAAAACA AGTTCCCACT GGGAATTTGA AGGTCCAACA
ATCATCTACT GTCCTTCTAG AAAAATGACA CAACAAGTTA CAGGTGAACT TAGGAAACTG
AATCTATCCT GTGGAACATA CCATGCGGGC ATGAGTTTTA GCACAAGGAA AGACATTCAT
CATAGGTTTG TAAGAGATGA AATTCAGTGT GTCATAGCTA CCATAGCTTT TGGAATGGGC
ATTAATAAAG CTGACATTCG CCAAGTCATT CATTACGGTG CTCCTAAGGA CATGGAATCA
TATTATCAGG AGATTGGTAG AGCTGGTCGT GATGGACTTC AAAGTTCTTG TCACGTCCTC
TGGGCTCCTG CAGACATTAA CTTAAATAGG CACCTTCTTA CTGAGATACG TAATGAGAAG
TTTCGATTAT ACAAATTAAA GATGATGGCA AAGATGGAAA AATATCTTCA TTCTAGCAGA
TGTAGGAGAC AAATCATCTT GTCTCATTTT GAGGACAAAC AAGTACAAAA AGCCTCCTTG
GGAATTATGG GAACTGAAAA ATGCTGTGAT AATTGCAGGT CCAGATTGGA TCATTGCTAT
TCCATGGATG ACTCAGAGGA TACATCCTGG GACTTTGGTC CACAAGCATT TAAGCTTTTG
TCTGCTGTGG ACATCTTAGG CGAAAAATTT GGAATTGGGC TTCCAATTTT ATTTCTCCGA
GGATCTAATT CTCAGCGTCT TGCCGATCAA TATCGCAGGC ACAGTTTATT TGGCACTGGC
AAGGATCAAA CAGAGAGTTG GTGGAAGGCT TTTTCCCGTC AGCTGATCAC TGAGGGATTC
TTGGTAGAAG TTTCTCGGTA TAACAAATTT ATGAAGATTT GCGCCCTTAC GAAAAAGGGT
AGAAATTGGC TTCATAAAGC TAATACAGAA TCTCAGAGCC TCATCCTTCA AGCTAATGAA
GAATTGTGTC CAAAGAAGTT GCTTCTGCCT AGTTCGAAAA CTGTATCTTC GGGCACCAAA
GAGCATTGTT ATAATCAAGT ACCAGTTGAA TTAAGTACAG AGAAGAAGTC TAACTTGGAG
AAGTTATATT CTTATAAACC ATGTGATAAG ATTTCTTCTG GGAGTAACAT TTCTAAAAAA
AGTATCATGG TACAGTCACC AGAAAAAGCT TACAGTTCCT CACAGCCTGT TATTTCGGCA
CAAGAGCAGG AGACTCAGAT TGTGTTATAT GGCAAATTGG TAGAAGCTAG GCAGAAACAT
GCCAATAAAA TGGATGTTCC CCCAGCTATT CTGGCAACAA ACAAGATACT GGTGGATATG
GCCAAAATGA GACCAACTAC GGTTGAAAAC GTAAAAAGGA TTGATGGTGT TTCTGAAGGC
AAAGCTGCCA TGTTGGCCCC TCTGTTGGAA GTCATCAAAC ATTTCTGCCA AACAAATAGT
GTTCAGACAG ACCTCTTTTC AAGTACAAAA CCTCAAGAAG AACAGAAGAC GAGTCTGGTA
GCAAAAAATA AAATATGCAC ACTTTCACAG TCTATGGCCA TCACATACTC TTTATTCCAA
GAAAAGAAGA TGCCTTTGAA GAGCATAGCT GAGAGCAGGA TTCTGCCTCT CATGACAATT
GGCATGCACT TATCCCAAGC GGTGAAAGCT GGCTGCCCCC TTGATTTGGA GCGAGCAGGC
CTGACTCCAG AGGTTCAGAA GATTATTGCT GATGTTATCC GAAACCCTCC CGTCAACTCA
GATATGAGTA AAATTAGCCT AATCAGAATG TTAGTTCCTG AAAACATTGA CACGTACCTT
ATCCACATGG CAATTGAGAT CCTTAAACAT GGTCCTGACA GCGGACTTCA ACCTTCATGT
GATGTCAACA AAAGGAGATG TTTTCCCGGT TCTGAAGAGA TCTGTTCAAG TTCTAAGAGA
AGCAAGGAAG AAGTAGGCAT CAATACTGAG ACTTCATCTG CAGAGAGAAA GAGACGATTA
CCTGTGTGGT TTGCCAAAGG AAGTGATACC AGCAAGAAAT TAATGGACAA AACGAAAAGG
GGAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61340
Clone ID Related Accession (Same CDS sequence) XM_011544640
Accession Version XM_011544640.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2700bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Werner syndrome ATP-dependent helicase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167187.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGATGAAAA TGAAGCTAAT GAAGGGGAAG AAGATGATGA TAAGGACTTT TTGTGGCCAG 
CACCCAATGA AGAGCAAGTT ACTTGCCTCA AGATGTACTT TGGCCATTCC AGTTTTAAAC
CGATATGGAA AGAGTTTGTG CTTCCAGTAT CCACCTGTTT ATGTAGGCAA GATTGGCCTT
GTTATCTCTC CCCTTATTTC TCTGATGGAA GACCAAGTGC TACAGCTTAA AATGTCCAAC
ATCCCAGCTT GCTTCCTTGG ATCAGCACAG TCAGAAAATG TTCTAACAGA TATTAAATTA
GGTAAATACC GGATTGTATA CGTAACTCCA GAATACTGTT CAGGTAACAT GGGCCTGCTC
CAGCAACTTG AGGCTGATAT TGGTATCACG CTCATTGCTG TGGATGAGGC TCACTGTATT
TCTGAGTGGG GGCATGATTT TAGGGATTCA TTCAGGAAGT TGGGCTCCCT AAAGACAGCA
CTGCCAATGG TTCCAATCGT TGCACTTACT GCTACTGCAA GTTCTTCAAT CCGGGAAGAC
ATTGTACGTT GCTTAAATCT GAGAAATCCT CAGATCACCT GTACTGGTTT TGATCGACCA
AACCTGTATT TAGAAGTTAG GCGAAAAACA GGGAATATCC TTCAGGATCT GCAGCCATTT
CTTGTCAAAA CAAGTTCCCA CTGGGAATTT GAAGGTCCAA CAATCATCTA CTGTCCTTCT
AGAAAAATGA CACAACAAGT TACAGGTGAA CTTAGGAAAC TGAATCTATC CTGTGGAACA
TACCATGCGG GCATGAGTTT TAGCACAAGG AAAGACATTC ATCATAGGTT TGTAAGAGAT
GAAATTCAGT GTGTCATAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT
CGCCAAGTCA TTCATTACGG TGCTCCTAAG GACATGGAAT CATATTATCA GGAGATTGGT
AGAGCTGGTC GTGATGGACT TCAAAGTTCT TGTCACGTCC TCTGGGCTCC TGCAGACATT
AACTTAAATA GGCACCTTCT TACTGAGATA CGTAATGAGA AGTTTCGATT ATACAAATTA
AAGATGATGG CAAAGATGGA AAAATATCTT CATTCTAGCA GATGTAGGAG ACAAATCATC
TTGTCTCATT TTGAGGACAA ACAAGTACAA AAAGCCTCCT TGGGAATTAT GGGAACTGAA
AAATGCTGTG ATAATTGCAG GTCCAGATTG GATCATTGCT ATTCCATGGA TGACTCAGAG
GATACATCCT GGGACTTTGG TCCACAAGCA TTTAAGCTTT TGTCTGCTGT GGACATCTTA
GGCGAAAAAT TTGGAATTGG GCTTCCAATT TTATTTCTCC GAGGATCTAA TTCTCAGCGT
CTTGCCGATC AATATCGCAG GCACAGTTTA TTTGGCACTG GCAAGGATCA AACAGAGAGT
TGGTGGAAGG CTTTTTCCCG TCAGCTGATC ACTGAGGGAT TCTTGGTAGA AGTTTCTCGG
TATAACAAAT TTATGAAGAT TTGCGCCCTT ACGAAAAAGG GTAGAAATTG GCTTCATAAA
GCTAATACAG AATCTCAGAG CCTCATCCTT CAAGCTAATG AAGAATTGTG TCCAAAGAAG
TTGCTTCTGC CTAGTTCGAA AACTGTATCT TCGGGCACCA AAGAGCATTG TTATAATCAA
GTACCAGTTG AATTAAGTAC AGAGAAGAAG TCTAACTTGG AGAAGTTATA TTCTTATAAA
CCATGTGATA AGATTTCTTC TGGGAGTAAC ATTTCTAAAA AAAGTATCAT GGTACAGTCA
CCAGAAAAAG CTTACAGTTC CTCACAGCCT GTTATTTCGG CACAAGAGCA GGAGACTCAG
ATTGTGTTAT ATGGCAAATT GGTAGAAGCT AGGCAGAAAC ATGCCAATAA AATGGATGTT
CCCCCAGCTA TTCTGGCAAC AAACAAGATA CTGGTGGATA TGGCCAAAAT GAGACCAACT
ACGGTTGAAA ACGTAAAAAG GATTGATGGT GTTTCTGAAG GCAAAGCTGC CATGTTGGCC
CCTCTGTTGG AAGTCATCAA ACATTTCTGC CAAACAAATA GTGTTCAGAC AGACCTCTTT
TCAAGTACAA AACCTCAAGA AGAACAGAAG ACGAGTCTGG TAGCAAAAAA TAAAATATGC
ACACTTTCAC AGTCTATGGC CATCACATAC TCTTTATTCC AAGAAAAGAA GATGCCTTTG
AAGAGCATAG CTGAGAGCAG GATTCTGCCT CTCATGACAA TTGGCATGCA CTTATCCCAA
GCGGTGAAAG CTGGCTGCCC CCTTGATTTG GAGCGAGCAG GCCTGACTCC AGAGGTTCAG
AAGATTATTG CTGATGTTAT CCGAAACCCT CCCGTCAACT CAGATATGAG TAAAATTAGC
CTAATCAGAA TGTTAGTTCC TGAAAACATT GACACGTACC TTATCCACAT GGCAATTGAG
ATCCTTAAAC ATGGTCCTGA CAGCGGACTT CAACCTTCAT GTGATGTCAA CAAAAGGAGA
TGTTTTCCCG GTTCTGAAGA GATCTGTTCA AGTTCTAAGA GAAGCAAGGA AGAAGTAGGC
ATCAATACTG AGACTTCATC TGCAGAGAGA AAGAGACGAT TACCTGTGTG GTTTGCCAAA
GGAAGTGATA CCAGCAAGAA ATTAATGGAC AAAACGAAAA GGGGAGGTCT TTTTAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542942.1
CDS101..2800
Misc Feature(1)224..1531(+)
Misc Feature(2)227..616(+)
Misc Feature(3)227..235(+)
Misc Feature(4)503..514(+)
Misc Feature(5)776..1102(+)
Misc Feature(6)815..970(+)
Misc Feature(7)986..1072(+)
Misc Feature(8)1373..1654(+)
Misc Feature(9)1967..2188(+)
Misc Feature(10)2270..2557(+)
Translation

Target ORF information:

RefSeq Version XM_011544640
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544640

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGATGAAAA TGAAGCTAAT GAAGGGGAAG AAGATGATGA TAAGGACTTT TTGTGGCCAG 
CACCCAATGA AGAGCAAGTT ACTTGCCTCA AGATGTACTT TGGCCATTCC AGTTTTAAAC
CGATATGGAA AGAGTTTGTG CTTCCAGTAT CCACCTGTTT ATGTAGGCAA GATTGGCCTT
GTTATCTCTC CCCTTATTTC TCTGATGGAA GACCAAGTGC TACAGCTTAA AATGTCCAAC
ATCCCAGCTT GCTTCCTTGG ATCAGCACAG TCAGAAAATG TTCTAACAGA TATTAAATTA
GGTAAATACC GGATTGTATA CGTAACTCCA GAATACTGTT CAGGTAACAT GGGCCTGCTC
CAGCAACTTG AGGCTGATAT TGGTATCACG CTCATTGCTG TGGATGAGGC TCACTGTATT
TCTGAGTGGG GGCATGATTT TAGGGATTCA TTCAGGAAGT TGGGCTCCCT AAAGACAGCA
CTGCCAATGG TTCCAATCGT TGCACTTACT GCTACTGCAA GTTCTTCAAT CCGGGAAGAC
ATTGTACGTT GCTTAAATCT GAGAAATCCT CAGATCACCT GTACTGGTTT TGATCGACCA
AACCTGTATT TAGAAGTTAG GCGAAAAACA GGGAATATCC TTCAGGATCT GCAGCCATTT
CTTGTCAAAA CAAGTTCCCA CTGGGAATTT GAAGGTCCAA CAATCATCTA CTGTCCTTCT
AGAAAAATGA CACAACAAGT TACAGGTGAA CTTAGGAAAC TGAATCTATC CTGTGGAACA
TACCATGCGG GCATGAGTTT TAGCACAAGG AAAGACATTC ATCATAGGTT TGTAAGAGAT
GAAATTCAGT GTGTCATAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT
CGCCAAGTCA TTCATTACGG TGCTCCTAAG GACATGGAAT CATATTATCA GGAGATTGGT
AGAGCTGGTC GTGATGGACT TCAAAGTTCT TGTCACGTCC TCTGGGCTCC TGCAGACATT
AACTTAAATA GGCACCTTCT TACTGAGATA CGTAATGAGA AGTTTCGATT ATACAAATTA
AAGATGATGG CAAAGATGGA AAAATATCTT CATTCTAGCA GATGTAGGAG ACAAATCATC
TTGTCTCATT TTGAGGACAA ACAAGTACAA AAAGCCTCCT TGGGAATTAT GGGAACTGAA
AAATGCTGTG ATAATTGCAG GTCCAGATTG GATCATTGCT ATTCCATGGA TGACTCAGAG
GATACATCCT GGGACTTTGG TCCACAAGCA TTTAAGCTTT TGTCTGCTGT GGACATCTTA
GGCGAAAAAT TTGGAATTGG GCTTCCAATT TTATTTCTCC GAGGATCTAA TTCTCAGCGT
CTTGCCGATC AATATCGCAG GCACAGTTTA TTTGGCACTG GCAAGGATCA AACAGAGAGT
TGGTGGAAGG CTTTTTCCCG TCAGCTGATC ACTGAGGGAT TCTTGGTAGA AGTTTCTCGG
TATAACAAAT TTATGAAGAT TTGCGCCCTT ACGAAAAAGG GTAGAAATTG GCTTCATAAA
GCTAATACAG AATCTCAGAG CCTCATCCTT CAAGCTAATG AAGAATTGTG TCCAAAGAAG
TTGCTTCTGC CTAGTTCGAA AACTGTATCT TCGGGCACCA AAGAGCATTG TTATAATCAA
GTACCAGTTG AATTAAGTAC AGAGAAGAAG TCTAACTTGG AGAAGTTATA TTCTTATAAA
CCATGTGATA AGATTTCTTC TGGGAGTAAC ATTTCTAAAA AAAGTATCAT GGTACAGTCA
CCAGAAAAAG CTTACAGTTC CTCACAGCCT GTTATTTCGG CACAAGAGCA GGAGACTCAG
ATTGTGTTAT ATGGCAAATT GGTAGAAGCT AGGCAGAAAC ATGCCAATAA AATGGATGTT
CCCCCAGCTA TTCTGGCAAC AAACAAGATA CTGGTGGATA TGGCCAAAAT GAGACCAACT
ACGGTTGAAA ACGTAAAAAG GATTGATGGT GTTTCTGAAG GCAAAGCTGC CATGTTGGCC
CCTCTGTTGG AAGTCATCAA ACATTTCTGC CAAACAAATA GTGTTCAGAC AGACCTCTTT
TCAAGTACAA AACCTCAAGA AGAACAGAAG ACGAGTCTGG TAGCAAAAAA TAAAATATGC
ACACTTTCAC AGTCTATGGC CATCACATAC TCTTTATTCC AAGAAAAGAA GATGCCTTTG
AAGAGCATAG CTGAGAGCAG GATTCTGCCT CTCATGACAA TTGGCATGCA CTTATCCCAA
GCGGTGAAAG CTGGCTGCCC CCTTGATTTG GAGCGAGCAG GCCTGACTCC AGAGGTTCAG
AAGATTATTG CTGATGTTAT CCGAAACCCT CCCGTCAACT CAGATATGAG TAAAATTAGC
CTAATCAGAA TGTTAGTTCC TGAAAACATT GACACGTACC TTATCCACAT GGCAATTGAG
ATCCTTAAAC ATGGTCCTGA CAGCGGACTT CAACCTTCAT GTGATGTCAA CAAAAGGAGA
TGTTTTCCCG GTTCTGAAGA GATCTGTTCA AGTTCTAAGA GAAGCAAGGA AGAAGTAGGC
ATCAATACTG AGACTTCATC TGCAGAGAGA AAGAGACGAT TACCTGTGTG GTTTGCCAAA
GGAAGTGATA CCAGCAAGAA ATTAATGGAC AAAACGAAAA GGGGAGGTCT TTTTAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18809
Clone ID Related Accession (Same CDS sequence) NM_000553
Accession Version NM_000553.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Werner syndrome ATP-dependent helicase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084736.5, BP279291.1, AF091214.1 and AB209652.1. This sequence is a reference standard in the RefSeqGene project. On Apr 11, 2008 this sequence version replaced gi:110735438. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTCGGAA GAGTGTTTTT
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA CGATGCTAGT
GATTGCTCTT TCCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACG TTTCTTCCAT GTCAGTTTTT
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGCACAGAGA CCTGGAGCCT TAACAGTCTG
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AATTGGAAAA CCCACGGAGG
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT
GAAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT
CACGTTGAAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA
GATGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT
GAACTCCAAA TTTTGGAACA GCAGTCTCAG GAAGAATATC TTAGTGATAT TGCTTATAAA
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT
GATGAAGATT TAGAAATGGA GATGCTTAAG CATTTATCTC CCAATGATAA TGAAAACGAT
ACGTCCTATG TAATTGAGAG TGATGAAGAT TTAGAAATGG AGATGCTTAA GTCTTTAGAA
AACCTCAATA GTGGCACGGT AGAACCAACT CATTCTAAAT GCTTAAAAAT GGAAAGAAAT
CTGGGTCTTC CTACTAAAGA AGAAGAAGAA GATGATGAAA ATGAAGCTAA TGAAGGGGAA
GAAGATGATG ATAAGGACTT TTTGTGGCCA GCACCCAATG AAGAGCAAGT TACTTGCCTC
AAGATGTACT TTGGCCATTC CAGTTTTAAA CCAGTTCAGT GGAAAGTGAT TCATTCAGTA
TTAGAAGAAA GAAGAGATAA TGTTGCTGTC ATGGCAACTG GATATGGAAA GAGTTTGTGC
TTCCAGTATC CACCTGTTTA TGTAGGCAAG ATTGGCCTTG TTATCTCTCC CCTTATTTCT
CTGATGGAAG ACCAAGTGCT ACAGCTTAAA ATGTCCAACA TCCCAGCTTG CTTCCTTGGA
TCAGCACAGT CAGAAAATGT TCTAACAGAT ATTAAATTAG GTAAATACCG GATTGTATAC
GTAACTCCAG AATACTGTTC AGGTAACATG GGCCTGCTCC AGCAACTTGA GGCTGATATT
GGTATCACGC TCATTGCTGT GGATGAGGCT CACTGTATTT CTGAGTGGGG GCATGATTTT
AGGGATTCAT TCAGGAAGTT GGGCTCCCTA AAGACAGCAC TGCCAATGGT TCCAATCGTT
GCACTTACTG CTACTGCAAG TTCTTCAATC CGGGAAGACA TTGTACGTTG CTTAAATCTG
AGAAATCCTC AGATCACCTG TACTGGTTTT GATCGACCAA ACCTGTATTT AGAAGTTAGG
CGAAAAACAG GGAATATCCT TCAGGATCTG CAGCCATTTC TTGTCAAAAC AAGTTCCCAC
TGGGAATTTG AAGGTCCAAC AATCATCTAC TGTCCTTCTA GAAAAATGAC ACAACAAGTT
ACAGGTGAAC TTAGGAAACT GAATCTATCC TGTGGAACAT ACCATGCGGG CATGAGTTTT
AGCACAAGGA AAGACATTCA TCATAGGTTT GTAAGAGATG AAATTCAGTG TGTCATAGCT
ACCATAGCTT TTGGAATGGG CATTAATAAA GCTGACATTC GCCAAGTCAT TCATTACGGT
GCTCCTAAGG ACATGGAATC ATATTATCAG GAGATTGGTA GAGCTGGTCG TGATGGACTT
CAAAGTTCTT GTCACGTCCT CTGGGCTCCT GCAGACATTA ACTTAAATAG GCACCTTCTT
ACTGAGATAC GTAATGAGAA GTTTCGATTA TACAAATTAA AGATGATGGC AAAGATGGAA
AAATATCTTC ATTCTAGCAG ATGTAGGAGA CAAATCATCT TGTCTCATTT TGAGGACAAA
CAAGTACAAA AAGCCTCCTT GGGAATTATG GGAACTGAAA AATGCTGTGA TAATTGCAGG
TCCAGATTGG ATCATTGCTA TTCCATGGAT GACTCAGAGG ATACATCCTG GGACTTTGGT
CCACAAGCAT TTAAGCTTTT GTCTGCTGTG GACATCTTAG GCGAAAAATT TGGAATTGGG
CTTCCAATTT TATTTCTCCG AGGATCTAAT TCTCAGCGTC TTGCCGATCA ATATCGCAGG
CACAGTTTAT TTGGCACTGG CAAGGATCAA ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT
CAGCTGATCA CTGAGGGATT CTTGGTAGAA GTTTCTCGGT ATAACAAATT TATGAAGATT
TGCGCCCTTA CGAAAAAGGG TAGAAATTGG CTTCATAAAG CTAATACAGA ATCTCAGAGC
CTCATCCTTC AAGCTAATGA AGAATTGTGT CCAAAGAAGT TGCTTCTGCC TAGTTCGAAA
ACTGTATCTT CGGGCACCAA AGAGCATTGT TATAATCAAG TACCAGTTGA ATTAAGTACA
GAGAAGAAGT CTAACTTGGA GAAGTTATAT TCTTATAAAC CATGTGATAA GATTTCTTCT
GGGAGTAACA TTTCTAAAAA AAGTATCATG GTACAGTCAC CAGAAAAAGC TTACAGTTCC
TCACAGCCTG TTATTTCGGC ACAAGAGCAG GAGACTCAGA TTGTGTTATA TGGCAAATTG
GTAGAAGCTA GGCAGAAACA TGCCAATAAA ATGGATGTTC CCCCAGCTAT TCTGGCAACA
AACAAGATAC TGGTGGATAT GGCCAAAATG AGACCAACTA CGGTTGAAAA CGTAAAAAGG
ATTGATGGTG TTTCTGAAGG CAAAGCTGCC ATGTTGGCCC CTCTGTTGGA AGTCATCAAA
CATTTCTGCC AAACAAATAG TGTTCAGACA GACCTCTTTT CAAGTACAAA ACCTCAAGAA
GAACAGAAGA CGAGTCTGGT AGCAAAAAAT AAAATATGCA CACTTTCACA GTCTATGGCC
ATCACATACT CTTTATTCCA AGAAAAGAAG ATGCCTTTGA AGAGCATAGC TGAGAGCAGG
ATTCTGCCTC TCATGACAAT TGGCATGCAC TTATCCCAAG CGGTGAAAGC TGGCTGCCCC
CTTGATTTGG AGCGAGCAGG CCTGACTCCA GAGGTTCAGA AGATTATTGC TGATGTTATC
CGAAACCCTC CCGTCAACTC AGATATGAGT AAAATTAGCC TAATCAGAAT GTTAGTTCCT
GAAAACATTG ACACGTACCT TATCCACATG GCAATTGAGA TCCTTAAACA TGGTCCTGAC
AGCGGACTTC AACCTTCATG TGATGTCAAC AAAAGGAGAT GTTTTCCCGG TTCTGAAGAG
ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA GAAGTAGGCA TCAATACTGA GACTTCATCT
GCAGAGAGAA AGAGACGATT ACCTGTGTGG TTTGCCAAAG GAAGTGATAC CAGCAAGAAA
TTAATGGACA AAACGAAAAG GGGAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000544.2
CDS789..5087
Misc Feature(1)774..776(+)
Misc Feature(2)792..1619(+)
Misc Feature(3)792..794(+)
Misc Feature(4)969..1475(+)
Misc Feature(5)1032..1436(+)
Misc Feature(6)1032..1436(+)
Misc Feature(7)1035..1436(+)
Misc Feature(8)1221..1223(+)
Misc Feature(9)2058..2219(+)
Misc Feature(10)2064..2066(+)
Misc Feature(11)2106..2108(+)
Misc Feature(12)2145..2147(+)
Misc Feature(13)2187..2189(+)
Misc Feature(14)2400..3818(+)
Misc Feature(15)2481..2903(+)
Misc Feature(16)2508..2522(+)
Misc Feature(17)2790..2801(+)
Misc Feature(18)2790..2801(+)
Misc Feature(19)3063..3389(+)
Misc Feature(20)3102..3257(+)
Misc Feature(21)3273..3359(+)
Misc Feature(22)3660..3941(+)
Misc Feature(23)3747..3767(+)
Misc Feature(24)3897..3899(+)
Misc Feature(25)3960..3962(+)
Misc Feature(26)4185..4187(+)
Misc Feature(27)4209..4211(+)
Misc Feature(28)4254..4475(+)
Misc Feature(29)4557..4844(+)
Misc Feature(30)4662..4664(+)
Exon (1)1..712
Gene:WRN
Gene Synonym:
Exon (2)713..884
Gene:WRN
Gene Synonym:
Exon (3)885..997
Gene:WRN
Gene Synonym:
Exon (4)998..1143
Gene:WRN
Gene Synonym:
Exon (5)1144..1292
Gene:WRN
Gene Synonym:
Exon (6)1293..1442
Gene:WRN
Gene Synonym:
Exon (7)1443..1512
Gene:WRN
Gene Synonym:
Exon (8)1513..1627
Gene:WRN
Gene Synonym:
Exon (9)1628..2057
Gene:WRN
Gene Synonym:
Exon (10)2058..2138
Gene:WRN
Gene Synonym:
Exon (11)2139..2219
Gene:WRN
Gene Synonym:
Exon (12)2220..2364
Gene:WRN
Gene Synonym:
Exon (13)2365..2440
Gene:WRN
Gene Synonym:
Exon (14)2441..2508
Gene:WRN
Gene Synonym:
Exon (15)2509..2617
Gene:WRN
Gene Synonym:
Exon (16)2618..2686
Gene:WRN
Gene Synonym:
Exon (17)2687..2769
Gene:WRN
Gene Synonym:
Exon (18)2770..2876
Gene:WRN
Gene Synonym:
Exon (19)2877..3061
Gene:WRN
Gene Synonym:
Exon (20)3062..3236
Gene:WRN
Gene Synonym:
Exon (21)3237..3418
Gene:WRN
Gene Synonym:
Exon (22)3419..3520
Gene:WRN
Gene Synonym:
Exon (23)3521..3613
Gene:WRN
Gene Synonym:
Exon (24)3614..3755
Gene:WRN
Gene Synonym:
Exon (25)3756..3926
Gene:WRN
Gene Synonym:
Exon (26)3927..4021
Gene:WRN
Gene Synonym:
Exon (27)4022..4097
Gene:WRN
Gene Synonym:
Exon (28)4098..4171
Gene:WRN
Gene Synonym:
Exon (29)4172..4247
Gene:WRN
Gene Synonym:
Exon (30)4248..4360
Gene:WRN
Gene Synonym:
Exon (31)4361..4475
Gene:WRN
Gene Synonym:
Exon (32)4476..4607
Gene:WRN
Gene Synonym:
Exon (33)4608..4770
Gene:WRN
Gene Synonym:
Exon (34)4771..4979
Gene:WRN
Gene Synonym:
Exon (35)4980..5746
Gene:WRN
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000553
Organism Homo sapiens (human)
Definition Homo sapiens Werner syndrome, RecQ helicase-like (WRN), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000553

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGAGTGAAA AAAAATTGGA AACAACTGCA CAGCAGCGGA AATGTCCTGA ATGGATGAAT 
GTGCAGAATA AAAGATGTGC TGTAGAAGAA AGAAAGGCAT GTGTTCGGAA GAGTGTTTTT
GAAGATGACC TCCCCTTCTT AGAATTCACT GGATCCATTG TGTATAGTTA CGATGCTAGT
GATTGCTCTT TCCTGTCAGA AGATATTAGC ATGAGTCTAT CAGATGGGGA TGTGGTGGGA
TTTGACATGG AGTGGCCACC ATTATACAAT AGAGGGAAAC TTGGCAAAGT TGCACTAATT
CAGTTGTGTG TTTCTGAGAG CAAATGTTAC TTGTTCCACG TTTCTTCCAT GTCAGTTTTT
CCCCAGGGAT TAAAAATGTT GCTTGAAAAT AAAGCAGTTA AAAAGGCAGG TGTAGGAATT
GAAGGAGATC AGTGGAAACT TCTACGTGAC TTTGATATCA AATTGAAGAA TTTTGTGGAG
TTGACAGATG TTGCCAATAA AAAGCTGAAA TGCACAGAGA CCTGGAGCCT TAACAGTCTG
GTTAAACACC TCTTAGGTAA ACAGCTCCTG AAAGACAAGT CTATCCGCTG TAGCAATTGG
AGTAAATTTC CTCTCACTGA GGACCAGAAA CTGTATGCAG CCACTGATGC TTATGCTGGT
TTTATTATTT ACCGAAATTT AGAGATTTTG GATGATACTG TGCAAAGGTT TGCTATAAAT
AAAGAGGAAG AAATCCTACT TAGCGACATG AACAAACAGT TGACTTCAAT CTCTGAGGAA
GTGATGGATC TGGCTAAGCA TCTTCCTCAT GCTTTCAGTA AATTGGAAAA CCCACGGAGG
GTTTCTATCT TACTAAAGGA TATTTCAGAA AATCTATATT CACTGAGGAG GATGATAATT
GGGTCTACTA ACATTGAGAC TGAACTGAGG CCCAGCAATA ATTTAAACTT ATTATCCTTT
GAAGATTCAA CTACTGGGGG AGTACAACAG AAACAAATTA GAGAACATGA AGTTTTAATT
CACGTTGAAG ATGAAACATG GGACCCAACA CTTGATCATT TAGCTAAACA TGATGGAGAA
GATGTACTTG GAAATAAAGT GGAACGAAAA GAAGATGGAT TTGAAGATGG AGTAGAAGAC
AACAAATTGA AAGAGAATAT GGAAAGAGCT TGTTTGATGT CGTTAGATAT TACAGAACAT
GAACTCCAAA TTTTGGAACA GCAGTCTCAG GAAGAATATC TTAGTGATAT TGCTTATAAA
TCTACTGAGC ATTTATCTCC CAATGATAAT GAAAACGATA CGTCCTATGT AATTGAGAGT
GATGAAGATT TAGAAATGGA GATGCTTAAG CATTTATCTC CCAATGATAA TGAAAACGAT
ACGTCCTATG TAATTGAGAG TGATGAAGAT TTAGAAATGG AGATGCTTAA GTCTTTAGAA
AACCTCAATA GTGGCACGGT AGAACCAACT CATTCTAAAT GCTTAAAAAT GGAAAGAAAT
CTGGGTCTTC CTACTAAAGA AGAAGAAGAA GATGATGAAA ATGAAGCTAA TGAAGGGGAA
GAAGATGATG ATAAGGACTT TTTGTGGCCA GCACCCAATG AAGAGCAAGT TACTTGCCTC
AAGATGTACT TTGGCCATTC CAGTTTTAAA CCAGTTCAGT GGAAAGTGAT TCATTCAGTA
TTAGAAGAAA GAAGAGATAA TGTTGCTGTC ATGGCAACTG GATATGGAAA GAGTTTGTGC
TTCCAGTATC CACCTGTTTA TGTAGGCAAG ATTGGCCTTG TTATCTCTCC CCTTATTTCT
CTGATGGAAG ACCAAGTGCT ACAGCTTAAA ATGTCCAACA TCCCAGCTTG CTTCCTTGGA
TCAGCACAGT CAGAAAATGT TCTAACAGAT ATTAAATTAG GTAAATACCG GATTGTATAC
GTAACTCCAG AATACTGTTC AGGTAACATG GGCCTGCTCC AGCAACTTGA GGCTGATATT
GGTATCACGC TCATTGCTGT GGATGAGGCT CACTGTATTT CTGAGTGGGG GCATGATTTT
AGGGATTCAT TCAGGAAGTT GGGCTCCCTA AAGACAGCAC TGCCAATGGT TCCAATCGTT
GCACTTACTG CTACTGCAAG TTCTTCAATC CGGGAAGACA TTGTACGTTG CTTAAATCTG
AGAAATCCTC AGATCACCTG TACTGGTTTT GATCGACCAA ACCTGTATTT AGAAGTTAGG
CGAAAAACAG GGAATATCCT TCAGGATCTG CAGCCATTTC TTGTCAAAAC AAGTTCCCAC
TGGGAATTTG AAGGTCCAAC AATCATCTAC TGTCCTTCTA GAAAAATGAC ACAACAAGTT
ACAGGTGAAC TTAGGAAACT GAATCTATCC TGTGGAACAT ACCATGCGGG CATGAGTTTT
AGCACAAGGA AAGACATTCA TCATAGGTTT GTAAGAGATG AAATTCAGTG TGTCATAGCT
ACCATAGCTT TTGGAATGGG CATTAATAAA GCTGACATTC GCCAAGTCAT TCATTACGGT
GCTCCTAAGG ACATGGAATC ATATTATCAG GAGATTGGTA GAGCTGGTCG TGATGGACTT
CAAAGTTCTT GTCACGTCCT CTGGGCTCCT GCAGACATTA ACTTAAATAG GCACCTTCTT
ACTGAGATAC GTAATGAGAA GTTTCGATTA TACAAATTAA AGATGATGGC AAAGATGGAA
AAATATCTTC ATTCTAGCAG ATGTAGGAGA CAAATCATCT TGTCTCATTT TGAGGACAAA
CAAGTACAAA AAGCCTCCTT GGGAATTATG GGAACTGAAA AATGCTGTGA TAATTGCAGG
TCCAGATTGG ATCATTGCTA TTCCATGGAT GACTCAGAGG ATACATCCTG GGACTTTGGT
CCACAAGCAT TTAAGCTTTT GTCTGCTGTG GACATCTTAG GCGAAAAATT TGGAATTGGG
CTTCCAATTT TATTTCTCCG AGGATCTAAT TCTCAGCGTC TTGCCGATCA ATATCGCAGG
CACAGTTTAT TTGGCACTGG CAAGGATCAA ACAGAGAGTT GGTGGAAGGC TTTTTCCCGT
CAGCTGATCA CTGAGGGATT CTTGGTAGAA GTTTCTCGGT ATAACAAATT TATGAAGATT
TGCGCCCTTA CGAAAAAGGG TAGAAATTGG CTTCATAAAG CTAATACAGA ATCTCAGAGC
CTCATCCTTC AAGCTAATGA AGAATTGTGT CCAAAGAAGT TGCTTCTGCC TAGTTCGAAA
ACTGTATCTT CGGGCACCAA AGAGCATTGT TATAATCAAG TACCAGTTGA ATTAAGTACA
GAGAAGAAGT CTAACTTGGA GAAGTTATAT TCTTATAAAC CATGTGATAA GATTTCTTCT
GGGAGTAACA TTTCTAAAAA AAGTATCATG GTACAGTCAC CAGAAAAAGC TTACAGTTCC
TCACAGCCTG TTATTTCGGC ACAAGAGCAG GAGACTCAGA TTGTGTTATA TGGCAAATTG
GTAGAAGCTA GGCAGAAACA TGCCAATAAA ATGGATGTTC CCCCAGCTAT TCTGGCAACA
AACAAGATAC TGGTGGATAT GGCCAAAATG AGACCAACTA CGGTTGAAAA CGTAAAAAGG
ATTGATGGTG TTTCTGAAGG CAAAGCTGCC ATGTTGGCCC CTCTGTTGGA AGTCATCAAA
CATTTCTGCC AAACAAATAG TGTTCAGACA GACCTCTTTT CAAGTACAAA ACCTCAAGAA
GAACAGAAGA CGAGTCTGGT AGCAAAAAAT AAAATATGCA CACTTTCACA GTCTATGGCC
ATCACATACT CTTTATTCCA AGAAAAGAAG ATGCCTTTGA AGAGCATAGC TGAGAGCAGG
ATTCTGCCTC TCATGACAAT TGGCATGCAC TTATCCCAAG CGGTGAAAGC TGGCTGCCCC
CTTGATTTGG AGCGAGCAGG CCTGACTCCA GAGGTTCAGA AGATTATTGC TGATGTTATC
CGAAACCCTC CCGTCAACTC AGATATGAGT AAAATTAGCC TAATCAGAAT GTTAGTTCCT
GAAAACATTG ACACGTACCT TATCCACATG GCAATTGAGA TCCTTAAACA TGGTCCTGAC
AGCGGACTTC AACCTTCATG TGATGTCAAC AAAAGGAGAT GTTTTCCCGG TTCTGAAGAG
ATCTGTTCAA GTTCTAAGAG AAGCAAGGAA GAAGTAGGCA TCAATACTGA GACTTCATCT
GCAGAGAGAA AGAGACGATT ACCTGTGTGG TTTGCCAAAG GAAGTGATAC CAGCAAGAAA
TTAATGGACA AAACGAAAAG GGGAGGTCTT TTTAGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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