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ZP2 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol ZP2
Entrez Gene ID 7783
Full Name zona pellucida glycoprotein 2
Synonyms ZPA,Zp-2
General protein information
Preferred Names

zona pellucida glycoprotein 2

Names

zona pellucida sperm-binding protein 2
zona pellucida glycoprotein 2 (sperm receptor)
zona pellucida glycoprotein ZP2
zona pellucida protein A
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

16

16p12.3-p12.2
Summary The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014].
Disorder MIM:

182888


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001376232.1 NP_001363161.1 zona pellucida sperm-binding protein 2 isoform 1 preproprotein
NM_001290104.1 NP_001277033.1 zona pellucida sperm-binding protein 2 isoform 2 preproprotein
XM_011545942.3 XP_011544244.2 zona pellucida sperm-binding protein 2 isoform X1
NM_001376231.1 NP_001363160.1 zona pellucida sperm-binding protein 2 isoform 2 preproprotein
XM_011545942.2 XP_011544244.1 zona pellucida sperm-binding protein 2 isoform X1
NM_003460.2 NP_003451.1 zona pellucida sperm-binding protein 2 isoform 1 preproprotein
NM_001376233.1 NP_001363162.1 zona pellucida sperm-binding protein 2 isoform 3 preproprotein

Pathways from BioSystems


Homologies


ZP2 XP_001091147.2
Canis lupus familiaris (dog) ZP2 NP_001003304.1
Gallus gallus (chicken) ZP2 NP_001034187.1
Xenopus tropicalis (tropical clawed frog) zp2 NP_988855.1
Pan troglodytes (chimpanzee) ZP2 XP_510869.3
Mus musculus (house mouse) Zp2 NP_035905.1
Homo sapiens (human) ZP2 NP_003451.1
Bos taurus (cattle) ZP2 NP_776398.1
Rattus norvegicus (Norway rat) Zp2 NP_112412.1

Gene Ontology


Bibliography

Related articles in PubMed

A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility.
Chen T, Bian Y, Liu X, Zhao S, Wu K, Yan L, Li M, Yang Z, Liu H, Zhao H, Chen ZJ
American journal of human genetics101(3)459-465(2017 Sep)

Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility.
Liu W, Li K, Bai D, Yin J, Tang Y, Chi F, Zhang L, Wang Y, Pan J, Liang S, Guo Y, Ruan J, Kou X, Zhao Y, Wang H, Chen J, Teng X, Gao S
Human genetics136(8)975-985(2017 Aug)

A common 'aggregation-prone' interface possibly participates in the self-assembly of human zona pellucida proteins.
Louros NN, Chrysina ED, Baltatzis GE, Patsouris ES, Hamodrakas SJ, Iconomidou VA
FEBS letters590(5)619-30(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in ZP2 and ZP3 have dosage effects which can cause female infertility in humans.
Title: Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility.

ZP2(51-149) sperm-binding domain is necessary for human gamete recognition and penetration through the zona pellucida.
Title: A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans.

observed sequence variations in exons of ZP2 gene in women with infertility of unknown origin who exhibit abnormal zona pellucida; sperm-ovum interactions appear relatively normal in these patients [CASE REPORTS]
Title: Genetic and physiological study of morphologically abnormal human zona pellucida.

Studies indicate that eggs from mice lacking an ortholog of ZP2 do not contain a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts.
Title: Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility.

ZP proteins were detected in both the oocyte and the granulosa cells as early as the primordial follicle stage in the human. The detection of ZP proteins in the quiescent primordial follicle suggests that these proteins have been present since oogenesis.
Title: Detection of zona pellucida proteins during human folliculogenesis.

The following ZP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28445 NM_003460.2
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $524.30
$749.00
OHu28459 NM_001290104.1
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu61480 XM_011545942.2
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu28445 NM_001376232.1
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu28459 NM_001376231.1
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu61480 NM_001376233.1
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu104202 XM_011545942.3
Latest version!		 Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu28445
Clone ID Related Accession (Same CDS sequence) NM_001376232.1 , NM_003460.2
Accession Version NM_003460.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-16
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform 1 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310454.1 and AF001550.1. On Feb 26, 2014 this sequence version replaced NM_003460.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGCCA CAGGGGCCAC TGAAGCAGAG AAAATGACAG TCAGCCTCCC AGGACCCATT 
CTCCTGTTGT CAGATGACTC CTCATTCAGA GGTGTCGGCT CATCTGATCT AAAAGCAAGT 
GGGAGCAGTG GGGAGAAGAG TAGGAGTGAA ACAGGGGAGG AGGTTGGCTC ACGAGGTGCT 
ATGGACACCA AAGGGCACAA GACTGCTGGA GATGTTGGTT CCAAAGCTGT GGCTGCTGTG 
GCTGCCTTTG CAGGTGTGGT GGCAACTCTA GGCTTCATCT ACTACCTGTA CGAGAAAAGG 
ACTGTGTCAA ATCACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003451.1
CDS484..2721
Translation

Target ORF information:

RefSeq Version NM_003460.2
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003460.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGCCA CAGGGGCCAC TGAAGCAGAG AAAATGACAG TCAGCCTCCC AGGACCCATT 
CTCCTGTTGT CAGATGACTC CTCATTCAGA GGTGTCGGCT CATCTGATCT AAAAGCAAGT 
GGGAGCAGTG GGGAGAAGAG TAGGAGTGAA ACAGGGGAGG AGGTTGGCTC ACGAGGTGCT 
ATGGACACCA AAGGGCACAA GACTGCTGGA GATGTTGGTT CCAAAGCTGT GGCTGCTGTG 
GCTGCCTTTG CAGGTGTGGT GGCAACTCTA GGCTTCATCT ACTACCTGTA CGAGAAAAGG 
ACTGTGTCAA ATCACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28459
Clone ID Related Accession (Same CDS sequence) NM_001290104.1 , NM_001376231.1
Accession Version NM_001290104.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-27
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform 2 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310454.1, AF001550.1 and BC096305.3. On Feb 26, 2014 this sequence version replaced XM_006721090.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC096305.3 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGG 
AATGACATGC TACTAAACAT CAACGTTGAA AGCCTTACTC CTCCAGTGGC CTCAGTGAAG 
TTGGGTCCAT TTACCTTGAT CCTGCAAAGC TACCCAGATA ATTCCTACCA ACAACCTTAT 
GGGGAAAACG AGTACCCTCT AGTGAGATTC CTCCGCCAAC CAATTTACAT GGAAGTGAGA 
GTCCTAAACA GGGATGACCC CAACATCAAG CTGGTCTTAG ATGACTGCTG GGCGACGTCC 
ACCATGGATC CAGACTCTTT CCCCCAGTGG AACGTTGTCG TGGATGGCTG TGCATATGAC 
CTGGACAACT ACCAGACCAC CTTCCATCCA GTCGGCTCCT CTGTGACCCA TCCTGATCAC 
TATCAGAGGT TTGACATGAA GGCTTTTGCC TTTGTATCAG AAGCCCACGT GCTCTCTAGC 
CTGGTCTACT TCCACTGCAG TGCCTTAATC TGTAATCGAC TCTCCCCTGA CTCCCCACTG 
TGTTCTGTGA CCTGCCCTGT GTCCTCTAGG CACAGGCGAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001277033.1
CDS484..2694
Translation

Target ORF information:

RefSeq Version NM_001290104.1
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001290104.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGG 
AATGACATGC TACTAAACAT CAACGTTGAA AGCCTTACTC CTCCAGTGGC CTCAGTGAAG 
TTGGGTCCAT TTACCTTGAT CCTGCAAAGC TACCCAGATA ATTCCTACCA ACAACCTTAT 
GGGGAAAACG AGTACCCTCT AGTGAGATTC CTCCGCCAAC CAATTTACAT GGAAGTGAGA 
GTCCTAAACA GGGATGACCC CAACATCAAG CTGGTCTTAG ATGACTGCTG GGCGACGTCC 
ACCATGGATC CAGACTCTTT CCCCCAGTGG AACGTTGTCG TGGATGGCTG TGCATATGAC 
CTGGACAACT ACCAGACCAC CTTCCATCCA GTCGGCTCCT CTGTGACCCA TCCTGATCAC 
TATCAGAGGT TTGACATGAA GGCTTTTGCC TTTGTATCAG AAGCCCACGT GCTCTCTAGC 
CTGGTCTACT TCCACTGCAG TGCCTTAATC TGTAATCGAC TCTCCCCTGA CTCCCCACTG 
TGTTCTGTGA CCTGCCCTGT GTCCTCTAGG CACAGGCGAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61480
Clone ID Related Accession (Same CDS sequence) XM_011545942.2 , NM_001376233.1
Accession Version XM_011545942.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGAGG CCAAACACAA ACTTGATCAT CTGTCTCCAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011544244.1
CDS13..2283
Translation

Target ORF information:

RefSeq Version XM_011545942.2
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011545942.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGAGG CCAAACACAA ACTTGATCAT CTGTCTCCAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28445
Clone ID Related Accession (Same CDS sequence) NM_001376232.1 , NM_003460.2
Accession Version NM_001376232.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2238bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-19
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform 1 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF001550.1 and AK313003.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M90366.1, AK313003.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGCCA CAGGGGCCAC TGAAGCAGAG AAAATGACAG TCAGCCTCCC AGGACCCATT 
CTCCTGTTGT CAGATGACTC CTCATTCAGA GGTGTCGGCT CATCTGATCT AAAAGCAAGT 
GGGAGCAGTG GGGAGAAGAG TAGGAGTGAA ACAGGGGAGG AGGTTGGCTC ACGAGGTGCT 
ATGGACACCA AAGGGCACAA GACTGCTGGA GATGTTGGTT CCAAAGCTGT GGCTGCTGTG 
GCTGCCTTTG CAGGTGTGGT GGCAACTCTA GGCTTCATCT ACTACCTGTA CGAGAAAAGG 
ACTGTGTCAA ATCACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001363161.1
CDS28..2265
Translation

Target ORF information:

RefSeq Version NM_001376232.1
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001376232.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGCCA CAGGGGCCAC TGAAGCAGAG AAAATGACAG TCAGCCTCCC AGGACCCATT 
CTCCTGTTGT CAGATGACTC CTCATTCAGA GGTGTCGGCT CATCTGATCT AAAAGCAAGT 
GGGAGCAGTG GGGAGAAGAG TAGGAGTGAA ACAGGGGAGG AGGTTGGCTC ACGAGGTGCT 
ATGGACACCA AAGGGCACAA GACTGCTGGA GATGTTGGTT CCAAAGCTGT GGCTGCTGTG 
GCTGCCTTTG CAGGTGTGGT GGCAACTCTA GGCTTCATCT ACTACCTGTA CGAGAAAAGG 
ACTGTGTCAA ATCACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28459
Clone ID Related Accession (Same CDS sequence) NM_001290104.1 , NM_001376231.1
Accession Version NM_001376231.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-19
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform 2 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF001550.1 and BC096305.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC096305.3 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGG 
AATGACATGC TACTAAACAT CAACGTTGAA AGCCTTACTC CTCCAGTGGC CTCAGTGAAG 
TTGGGTCCAT TTACCTTGAT CCTGCAAAGC TACCCAGATA ATTCCTACCA ACAACCTTAT 
GGGGAAAACG AGTACCCTCT AGTGAGATTC CTCCGCCAAC CAATTTACAT GGAAGTGAGA 
GTCCTAAACA GGGATGACCC CAACATCAAG CTGGTCTTAG ATGACTGCTG GGCGACGTCC 
ACCATGGATC CAGACTCTTT CCCCCAGTGG AACGTTGTCG TGGATGGCTG TGCATATGAC 
CTGGACAACT ACCAGACCAC CTTCCATCCA GTCGGCTCCT CTGTGACCCA TCCTGATCAC 
TATCAGAGGT TTGACATGAA GGCTTTTGCC TTTGTATCAG AAGCCCACGT GCTCTCTAGC 
CTGGTCTACT TCCACTGCAG TGCCTTAATC TGTAATCGAC TCTCCCCTGA CTCCCCACTG 
TGTTCTGTGA CCTGCCCTGT GTCCTCTAGG CACAGGCGAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001363160.1
CDS28..2238
Translation

Target ORF information:

RefSeq Version NM_001376231.1
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001376231.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGG 
AATGACATGC TACTAAACAT CAACGTTGAA AGCCTTACTC CTCCAGTGGC CTCAGTGAAG 
TTGGGTCCAT TTACCTTGAT CCTGCAAAGC TACCCAGATA ATTCCTACCA ACAACCTTAT 
GGGGAAAACG AGTACCCTCT AGTGAGATTC CTCCGCCAAC CAATTTACAT GGAAGTGAGA 
GTCCTAAACA GGGATGACCC CAACATCAAG CTGGTCTTAG ATGACTGCTG GGCGACGTCC 
ACCATGGATC CAGACTCTTT CCCCCAGTGG AACGTTGTCG TGGATGGCTG TGCATATGAC 
CTGGACAACT ACCAGACCAC CTTCCATCCA GTCGGCTCCT CTGTGACCCA TCCTGATCAC 
TATCAGAGGT TTGACATGAA GGCTTTTGCC TTTGTATCAG AAGCCCACGT GCTCTCTAGC 
CTGGTCTACT TCCACTGCAG TGCCTTAATC TGTAATCGAC TCTCCCCTGA CTCCCCACTG 
TGTTCTGTGA CCTGCCCTGT GTCCTCTAGG CACAGGCGAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu61480
Clone ID Related Accession (Same CDS sequence) XM_011545942.2 , NM_001376233.1
Accession Version NM_001376233.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-17
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform 3 preproprotein
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF001550.1. On Nov 12, 2019 this sequence version replaced XM_011545942.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.204459.1, SRR1803611.104943.1 [ECO:0000332] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGAGG CCAAACACAA ACTTGATCAT CTGTCTCCAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001363162.1
CDS28..2298
Translation

Target ORF information:

RefSeq Version NM_001376233.1
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001376233.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCGTGCA GGCAGAGAGG AGGCTCTTGG AGTCCCTCAG GCTGGTTCAA TGCAGGCTGG 
AGCACCTACA GGTCGATTTC TCTCTTCTTC GCCCTTGTGA CTTCAGGGAA CTCCATAGAT 
GTTTCTCAGT TGGTAAATCC TGCCTTTCCA GGCACTGTCA CTTGCGATGA AAGGGAAATA 
ACAGTGGAGT TCCCAAGCAG TCCTGGCACC AAGAAATGGC ATGCATCTGT GGTGGATCCT 
CTTGGTCTCG ACATGCCGAA CTGCACTTAC ATCCTGGACC CAGAAAAGCT CACCCTGAGG 
GCTACCTATG ATAACTGTAC CAGGAGAGTG CATGGTGGAC ACCAGATGAC CATCAGAGTC 
ATGAACAACA GTGCTGCCTT AAGACACGGA GCTGTCATGT ATCAGTTCTT CTGTCCAGCT 
ATGCAAGTAG AAGAGACCCA GGGGCTTTCA GCATCTACAA TCTGCCAGAA GGATTTCATG 
TCTTTTTCCT TGCCACGGGT CTTCTCTGGC TTGGCTGACG ACAGTAAGGG GACCAAAGTT 
CAGATGGGAT GGAGCATTGA GGTTGGTGAT GGTGCAAGAG CCAAAACTCT GACCCTGCCA 
GAGGCCATGA AGGAAGGCTT CAGCCTCTTG ATTGACAACC ACAGGATGAC CTTCCATGTG 
CCATTCAATG CCACTGGAGT GACTCACTAT GTGCAAGGTA ACAGTCATCT CTACATGGTG 
TCTCTGAAGC TTACATTTAT ATCTCCTGGA CAGAAGGTGA TCTTCTCTTC ACAAGCTATT 
TGTGCACCAG ATCCTGTGAC CTGCAATGCC ACACACATGA CTCTCACCAT ACCAGAGTTT 
CCTGGGAAGC TTAAGTCTGT GAGCTTTGAA AACCAGAACA TTGATGTGAG CCAGCTGCAT 
GACAATGGAA TTGATCTAGA AGCAACAAAT GGCATGAAAT TGCATTTCAG CAAAACTCTG 
CTCAAAACGA AATTATCTGA AAAATGCCTA CTCCATCAGT TCTACTTAGC TTCACTCAAG 
CTGACCTTTC TCCTTCGGCC AGAGACAGTA TCCATGGTGA TCTATCCTGA GTGTCTCTGT 
GAGTCACCCG TTTCTATAGT TACAGGGGAG CTGTGCACCC AGGATGGGTT TATGGACGTC 
GAGGTCTACA GCTACCAAAC ACAACCAGCT CTTGACCTGG GTACTCTGAG GGTGGGAAAC 
TCATCCTGCC AGCCTGTCTT TGAGGCTCAG TCTCAGGGGC TGGTACGGTT CCACATACCC 
CTGAATGGAT GTGGAACGAG ATATAAGTTC GAAGATGATA AAGTCGTCTA TGAAAACGAA 
ATACATGCTC TCTGGACGGA TTTTCCTCCA AGCAAAATAT CTAGAGACAG TGAGTTCAGA 
ATGACAGTGA AGTGTTCTTA TAGCAGGAAT GACATGCTAC TAAACATCAA CGTTGAAAGC 
CTTACTCCTC CAGTGGCCTC AGTGAAGTTG GGTCCATTTA CCTTGATCCT GCAAAGCTAC 
CCAGATAATT CCTACCAACA ACCTTATGGG GAAAACGAGT ACCCTCTAGT GAGATTCCTC 
CGCCAACCAA TTTACATGGA AGTGAGAGTC CTAAACAGGG ATGACCCCAA CATCAAGCTG 
GTCTTAGATG ACTGCTGGGC GACGTCCACC ATGGATCCAG ACTCTTTCCC CCAGTGGAAC 
GTTGTCGTGG ATGGCTGTGC ATATGACCTG GACAACTACC AGACCACCTT CCATCCAGTC 
GGCTCCTCTG TGACCCATCC TGATCACTAT CAGAGGTTTG ACATGAAGGC TTTTGCCTTT 
GTATCAGAAG CCCACGTGCT CTCTAGCCTG GTCTACTTCC ACTGCAGTGC CTTAATCTGT 
AATCGACTCT CCCCTGACTC CCCACTGTGT TCTGTGACCT GCCCTGTGTC CTCTAGGCAC 
AGGCGAGAGG CCAAACACAA ACTTGATCAT CTGTCTCCAG CCACAGGGGC CACTGAAGCA 
GAGAAAATGA CAGTCAGCCT CCCAGGACCC ATTCTCCTGT TGTCAGATGA CTCCTCATTC 
AGAGGTGTCG GCTCATCTGA TCTAAAAGCA AGTGGGAGCA GTGGGGAGAA GAGTAGGAGT 
GAAACAGGGG AGGAGGTTGG CTCACGAGGT GCTATGGACA CCAAAGGGCA CAAGACTGCT 
GGAGATGTTG GTTCCAAAGC TGTGGCTGCT GTGGCTGCCT TTGCAGGTGT GGTGGCAACT 
CTAGGCTTCA TCTACTACCT GTACGAGAAA AGGACTGTGT CAAATCACTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu104202
Clone ID Related Accession (Same CDS sequence) XM_011545942.3
Accession Version XM_011545942.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-12
Organism Homo sapiens(human)
Product zona pellucida sperm-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545942.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGACAAG ATTCAGGTGC CCCCAATGGG GAGAGGGTGT GGATGCGGCT GGCTTCCTGC 
TATAAAAACA CAGAGCAGCC TACCCTCTAC CTGGTTGATC TGGTAGTGTC TTCTGCTATG 
GCGTGCAGGC AGAGAGGAGG CTCTTGGAGT CCCTCAGGCT GGTTCAATGC AGGCTGGAGC 
ACCTACAGGT CGATTTCTCT CTTCTTCGCC CTTGTGACTT CAGGGAACTC CATAGATGTT 
TCTCAGTTGG TAAATCCTGC CTTTCCAGGC ACTGTCACTT GCGATGAAAG GGAAATAACA 
GTGGAGTTCC CAAGCAGTCC TGGCACCAAG AAATGGCATG CATCTGTGGT GGATCCTCTT 
GGTCTCGACA TGCCGAACTG CACTTACATC CTGGACCCAG AAAAGCTCAC CCTGAGGGCT 
ACCTATGATA ACTGTACCAG GAGAGTGCAT GGTGGACACC AGATGACCAT CAGAGTCATG 
AACAACAGTG CTGCCTTAAG ACACGGAGCT GTCATGTATC AGTTCTTCTG TCCAGCTATG 
CAAGTAGAAG AGACCCAGGG GCTTTCAGCA TCTACAATCT GCCAGAAGGA TTTCATGTCT 
TTTTCCTTGC CACGGGTCTT CTCTGGCTTG GCTGACGACA GTAAGGGGAC CAAAGTTCAG 
ATGGGATGGA GCATTGAGGT TGGTGATGGT GCAAGAGCCA AAACTCTGAC CCTGCCAGAG 
GCCATGAAGG AAGGCTTCAG CCTCTTGATT GACAACCACA GGATGACCTT CCATGTGCCA 
TTCAATGCCA CTGGAGTGAC TCACTATGTG CAAGGTAACA GTCATCTCTA CATGGTGTCT 
CTGAAGCTTA CATTTATATC TCCTGGACAG AAGGTGATCT TCTCTTCACA AGCTATTTGT 
GCACCAGATC CTGTGACCTG CAATGCCACA CACATGACTC TCACCATACC AGAGTTTCCT 
GGGAAGCTTA AGTCTGTGAG CTTTGAAAAC CAGAACATTG ATGTGAGCCA GCTGCATGAC 
AATGGAATTG ATCTAGAAGC AACAAATGGC ATGAAATTGC ATTTCAGCAA AACTCTGCTC 
AAAACGAAAT TATCTGAAAA ATGCCTACTC CATCAGTTCT ACTTAGCTTC ACTCAAGCTG 
ACCTTTCTCC TTCGGCCAGA GACAGTATCC ATGGTGATCT ATCCTGAGTG TCTCTGTGAG 
TCACCCGTTT CTATAGTTAC AGGGGAGCTG TGCACCCAGG ATGGGTTTAT GGACGTCGAG 
GTCTACAGCT ACCAAACACA ACCAGCTCTT GACCTGGGTA CTCTGAGGGT GGGAAACTCA 
TCCTGCCAGC CTGTCTTTGA GGCTCAGTCT CAGGGGCTGG TACGGTTCCA CATACCCCTG 
AATGGATGTG GAACGAGATA TAAGTTCGAA GATGATAAAG TCGTCTATGA AAACGAAATA 
CATGCTCTCT GGACGGATTT TCCTCCAAGC AAAATATCTA GAGACAGTGA GTTCAGAATG 
ACAGTGAAGT GTTCTTATAG CAGGAATGAC ATGCTACTAA ACATCAACGT TGAAAGCCTT 
ACTCCTCCAG TGGCCTCAGT GAAGTTGGGT CCATTTACCT TGATCCTGCA AAGCTACCCA 
GATAATTCCT ACCAACAACC TTATGGGGAA AACGAGTACC CTCTAGTGAG ATTCCTCCGC 
CAACCAATTT ACATGGAAGT GAGAGTCCTA AACAGGGATG ACCCCAACAT CAAGCTGGTC 
TTAGATGACT GCTGGGCGAC GTCCACCATG GATCCAGACT CTTTCCCCCA GTGGAACGTT 
GTCGTGGATG GCTGTGCATA TGACCTGGAC AACTACCAGA CCACCTTCCA TCCAGTCGGC 
TCCTCTGTGA CCCATCCTGA TCACTATCAG AGGTTTGACA TGAAGGCTTT TGCCTTTGTA 
TCAGAAGCCC ACGTGCTCTC TAGCCTGGTC TACTTCCACT GCAGTGCCTT AATCTGTAAT 
CGACTCTCCC CTGACTCCCC ACTGTGTTCT GTGACCTGCC CTGTGTCCTC TAGGCACAGG 
CGAGAGGCCA AACACAAACT TGATCATCTG TCTCCAGCCA CAGGGGCCAC TGAAGCAGAG 
AAAATGACAG TCAGCCTCCC AGGACCCATT CTCCTGTTGT CAGATGACTC CTCATTCAGA 
GGTGTCGGCT CATCTGATCT AAAAGCAAGT GGGAGCAGTG GGGAGAAGAG TAGGAGTGAA 
ACAGGGGAGG AGGTTGGCTC ACGAGGTGCT ATGGACACCA AAGGGCACAA GACTGCTGGA 
GATGTTGGTT CCAAAGCTGT GGCTGCTGTG GCTGCCTTTG CAGGTGTGGT GGCAACTCTA 
GGCTTCATCT ACTACCTGTA CGAGAAAAGG ACTGTGTCAA ATCACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011544244.2
CDS78..2465
Translation

Target ORF information:

RefSeq Version XM_011545942.3
Organism Homo sapiens(human)
Definition Homo sapiens zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011545942.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGGACAAG ATTCAGGTGC CCCCAATGGG GAGAGGGTGT GGATGCGGCT GGCTTCCTGC 
TATAAAAACA CAGAGCAGCC TACCCTCTAC CTGGTTGATC TGGTAGTGTC TTCTGCTATG 
GCGTGCAGGC AGAGAGGAGG CTCTTGGAGT CCCTCAGGCT GGTTCAATGC AGGCTGGAGC 
ACCTACAGGT CGATTTCTCT CTTCTTCGCC CTTGTGACTT CAGGGAACTC CATAGATGTT 
TCTCAGTTGG TAAATCCTGC CTTTCCAGGC ACTGTCACTT GCGATGAAAG GGAAATAACA 
GTGGAGTTCC CAAGCAGTCC TGGCACCAAG AAATGGCATG CATCTGTGGT GGATCCTCTT 
GGTCTCGACA TGCCGAACTG CACTTACATC CTGGACCCAG AAAAGCTCAC CCTGAGGGCT 
ACCTATGATA ACTGTACCAG GAGAGTGCAT GGTGGACACC AGATGACCAT CAGAGTCATG 
AACAACAGTG CTGCCTTAAG ACACGGAGCT GTCATGTATC AGTTCTTCTG TCCAGCTATG 
CAAGTAGAAG AGACCCAGGG GCTTTCAGCA TCTACAATCT GCCAGAAGGA TTTCATGTCT 
TTTTCCTTGC CACGGGTCTT CTCTGGCTTG GCTGACGACA GTAAGGGGAC CAAAGTTCAG 
ATGGGATGGA GCATTGAGGT TGGTGATGGT GCAAGAGCCA AAACTCTGAC CCTGCCAGAG 
GCCATGAAGG AAGGCTTCAG CCTCTTGATT GACAACCACA GGATGACCTT CCATGTGCCA 
TTCAATGCCA CTGGAGTGAC TCACTATGTG CAAGGTAACA GTCATCTCTA CATGGTGTCT 
CTGAAGCTTA CATTTATATC TCCTGGACAG AAGGTGATCT TCTCTTCACA AGCTATTTGT 
GCACCAGATC CTGTGACCTG CAATGCCACA CACATGACTC TCACCATACC AGAGTTTCCT 
GGGAAGCTTA AGTCTGTGAG CTTTGAAAAC CAGAACATTG ATGTGAGCCA GCTGCATGAC 
AATGGAATTG ATCTAGAAGC AACAAATGGC ATGAAATTGC ATTTCAGCAA AACTCTGCTC 
AAAACGAAAT TATCTGAAAA ATGCCTACTC CATCAGTTCT ACTTAGCTTC ACTCAAGCTG 
ACCTTTCTCC TTCGGCCAGA GACAGTATCC ATGGTGATCT ATCCTGAGTG TCTCTGTGAG 
TCACCCGTTT CTATAGTTAC AGGGGAGCTG TGCACCCAGG ATGGGTTTAT GGACGTCGAG 
GTCTACAGCT ACCAAACACA ACCAGCTCTT GACCTGGGTA CTCTGAGGGT GGGAAACTCA 
TCCTGCCAGC CTGTCTTTGA GGCTCAGTCT CAGGGGCTGG TACGGTTCCA CATACCCCTG 
AATGGATGTG GAACGAGATA TAAGTTCGAA GATGATAAAG TCGTCTATGA AAACGAAATA 
CATGCTCTCT GGACGGATTT TCCTCCAAGC AAAATATCTA GAGACAGTGA GTTCAGAATG 
ACAGTGAAGT GTTCTTATAG CAGGAATGAC ATGCTACTAA ACATCAACGT TGAAAGCCTT 
ACTCCTCCAG TGGCCTCAGT GAAGTTGGGT CCATTTACCT TGATCCTGCA AAGCTACCCA 
GATAATTCCT ACCAACAACC TTATGGGGAA AACGAGTACC CTCTAGTGAG ATTCCTCCGC 
CAACCAATTT ACATGGAAGT GAGAGTCCTA AACAGGGATG ACCCCAACAT CAAGCTGGTC 
TTAGATGACT GCTGGGCGAC GTCCACCATG GATCCAGACT CTTTCCCCCA GTGGAACGTT 
GTCGTGGATG GCTGTGCATA TGACCTGGAC AACTACCAGA CCACCTTCCA TCCAGTCGGC 
TCCTCTGTGA CCCATCCTGA TCACTATCAG AGGTTTGACA TGAAGGCTTT TGCCTTTGTA 
TCAGAAGCCC ACGTGCTCTC TAGCCTGGTC TACTTCCACT GCAGTGCCTT AATCTGTAAT 
CGACTCTCCC CTGACTCCCC ACTGTGTTCT GTGACCTGCC CTGTGTCCTC TAGGCACAGG 
CGAGAGGCCA AACACAAACT TGATCATCTG TCTCCAGCCA CAGGGGCCAC TGAAGCAGAG 
AAAATGACAG TCAGCCTCCC AGGACCCATT CTCCTGTTGT CAGATGACTC CTCATTCAGA 
GGTGTCGGCT CATCTGATCT AAAAGCAAGT GGGAGCAGTG GGGAGAAGAG TAGGAGTGAA 
ACAGGGGAGG AGGTTGGCTC ACGAGGTGCT ATGGACACCA AAGGGCACAA GACTGCTGGA 
GATGTTGGTT CCAAAGCTGT GGCTGCTGTG GCTGCCTTTG CAGGTGTGGT GGCAACTCTA 
GGCTTCATCT ACTACCTGTA CGAGAAAAGG ACTGTGTCAA ATCACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility.
American journal of human genetics101(3)459-465(2017 Sep)
Chen T,Bian Y,Liu X,Zhao S,Wu K,Yan L,Li M,Yang Z,Liu H,Zhao H,Chen ZJ


book

Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility.
Human genetics136(8)975-985(2017 Aug)
Liu W,Li K,Bai D,Yin J,Tang Y,Chi F,Zhang L,Wang Y,Pan J,Liang S,Guo Y,Ruan J,Kou X,Zhao Y,Wang H,Chen J,Teng X,Gao S


book

A common 'aggregation-prone' interface possibly participates in the self-assembly of human zona pellucida proteins.
FEBS letters590(5)619-30(2016 Mar)
Louros NN,Chrysina ED,Baltatzis GE,Patsouris ES,Hamodrakas SJ,Iconomidou VA