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ZP3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ZP3
Entrez Gene ID 7784
Full Name zona pellucida glycoprotein 3
Synonyms ZP3A,ZP3B,ZPC,Zp-3
General protein information
Preferred Names

zona pellucida glycoprotein 3

Names

zona pellucida sperm-binding protein 3
ZP3A/ZP3B
sperm receptor
zona pellucida glycoprotein 3B
zona pellucida glycoprotein ZP3
zona pellucida protein C

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

7

7q11.23

Summary The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

182889


mRNA and Protein(s)

mRNA Protein Name
XM_017012586.1 XP_016868075.1 zona pellucida sperm-binding protein 3 isoform X1
NM_001110354.1 NP_001103824.1 zona pellucida sperm-binding protein 3 isoform 1 precursor
NM_007155.5 NP_009086.4 zona pellucida sperm-binding protein 3 isoform 2



Macaca mulatta (rhesus monkey) ZP3 XP_002803282.1
Bos taurus (cattle) ZP3 NP_776399.1
Gallus gallus (chicken) ZP3L2 XP_413701.4
Mus musculus (house mouse) Zp3 NP_035906.1
Rattus norvegicus (Norway rat) Zp3 NP_446214.1
Xenopus tropicalis (tropical clawed frog) LOC100495051 XP_004913254.1
Canis lupus familiaris (dog) ZP3 NP_001003224.1
Gallus gallus (chicken) ZP3 NP_989720.3
Danio rerio (zebrafish) LOC100536860 XP_003200340.1
Danio rerio (zebrafish) zgc:173556 NP_001103212.1
Danio rerio (zebrafish) zp3 NP_571406.1
Xenopus tropicalis (tropical clawed frog) LOC394444 NP_988853.1
Homo sapiens (human) ZP3 NP_001103824.1
Pan troglodytes (chimpanzee) ZP3 XP_528035.3
Danio rerio (zebrafish) zp3a.1 NP_001013289.1
Danio rerio (zebrafish) zp3a.2 NP_001025291.1
Gallus gallus (chicken) ZP3L1 XP_413693.2
Danio rerio (zebrafish) LOC100148225 XP_005169843.1
Danio rerio (zebrafish) zp3.2 NP_001020357.1
Danio rerio (zebrafish) zgc:171779 NP_001103303.1


Related articles in PubMed

Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3.
Maldera JA, Weigel Muñoz M, Chirinos M, Busso D, G E Raffo F, Battistone MA, Blaquier JA, Larrea F, Cuasnicu PS
Molecular human reproduction20(4)341-9(2014 Apr)

Genetic and physiological study of morphologically abnormal human zona pellucida.
Margalit M, Paz G, Yavetz H, Yogev L, Amit A, Hevlin-Schwartz T, Gupta SK, Kleiman SE
European journal of obstetrics, gynecology, and reproductive biology165(1)70-6(2012 Nov)

Recombinant human ZP3-induced sperm acrosome reaction: evidence for the involvement of T- and L-type voltage-gated calcium channels.
José O, Hernández-Hernández O, Chirinos M, González-González ME, Larrea F, Almanza A, Felix R, Darszon A, Treviño CL
Biochemical and biophysical research communications395(4)530-4(2010 May)

New genetic associations detected in a host response study to hepatitis B vaccine.
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M
Genes and immunity11(3)232-8(2010 Apr)

Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system.
Naz RK, Dhandapani L
Journal of reproductive immunology84(1)24-31(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in ZP2 and ZP3 have dosage effects which can cause female infertility in humans.
Title: Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility.

Epididymal CRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3.
Title: Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3.

observed sequence variations in exons of ZP3 gene in women with infertility of unknown origin who exhibit abnormal zona pellucida; sperm-ovum interactions appear relatively normal in these patients [CASE REPORTS]
Title: Genetic and physiological study of morphologically abnormal human zona pellucida.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

The functional activity of human ZP3 resides in its C-terminal domain.
Title: Functional activity of human ZP3 primary sperm receptor resides toward its C-terminus.

The following ZP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18037 NM_007155.5
Latest version!
Homo sapiens zona pellucida glycoprotein 3 (ZP3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu31830 NM_001110354.1
Latest version!
Homo sapiens zona pellucida glycoprotein 3 (ZP3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu97484 XM_017012586.1
Latest version!
Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18037
Clone ID Related Accession (Same CDS sequence) NM_007155.5
Accession Version NM_007155.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product zona pellucida sperm-binding protein 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292763.1, DB328313.1 and BC113949.1. This sequence is a reference standard in the RefSeqGene project. On Nov 16, 2007 this sequence version replaced NM_007155.4. Transcript Variant: This variant (2) differs in the 5' UTR and 5' CDS compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292763.1, BC113949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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721
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841
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961
1021
1081
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGAT ATACATCACC TGCCACCTGA AGGTCACCCT AGCTGAGCAG
GACCCAGATG AACTCAACAA GGCCTGTTCC TTCAGCAAGC CTTCCAACAG CTGGTTCCCA
GTGGAAGGCT CGGCTGACAT CTGTCAATGC TGTAACAAAG GTGACTGTGG CACTCCAAGC
CATTCCAGGA GGCAGCCTCA TGTCATGAGC CAGTGGTCCA GGTCTGCTTC CCGTAACCGC
AGGCATGTGA CAGAAGAAGC AGATGTCACC GTGGGGCCAC TGATCTTCCT GGACAGGAGG
GGTGACCATG AAGTAGAGCA GTGGGCTTTG CCTTCTGACA CCTCAGTGGT GCTGCTGGGC
GTAGGCCTGG CTGTGGTGGT GTCCCTGACT CTGACTGCTG TTATCCTGGT TCTCACCAGG
AGGTGTCGCA CTGCCTCCCA CCCTGTGTCT GCTTCCGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_009086.4
CDS341..1462
Misc Feature(1)344..1096
Misc Feature(2)<746..1090
Misc Feature(3)1241..1243
Exon (1)1..274
Exon (2)275..499
Exon (3)500..618
Exon (4)619..722
Exon (5)723..900
Exon (6)901..1018
Exon (7)1019..1110
Exon (8)1111..1247
Exon (9)1248..1477
Translation

Target ORF information:

RefSeq Version NM_007155.5
Organism Homo sapiens(Human)
Definition Homo sapiens zona pellucida glycoprotein 3 (ZP3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007155.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGAT ATACATCACC TGCCACCTGA AGGTCACCCT AGCTGAGCAG
GACCCAGATG AACTCAACAA GGCCTGTTCC TTCAGCAAGC CTTCCAACAG CTGGTTCCCA
GTGGAAGGCT CGGCTGACAT CTGTCAATGC TGTAACAAAG GTGACTGTGG CACTCCAAGC
CATTCCAGGA GGCAGCCTCA TGTCATGAGC CAGTGGTCCA GGTCTGCTTC CCGTAACCGC
AGGCATGTGA CAGAAGAAGC AGATGTCACC GTGGGGCCAC TGATCTTCCT GGACAGGAGG
GGTGACCATG AAGTAGAGCA GTGGGCTTTG CCTTCTGACA CCTCAGTGGT GCTGCTGGGC
GTAGGCCTGG CTGTGGTGGT GTCCCTGACT CTGACTGCTG TTATCCTGGT TCTCACCAGG
AGGTGTCGCA CTGCCTCCCA CCCTGTGTCT GCTTCCGAAT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31830
Clone ID Related Accession (Same CDS sequence) NM_001110354.1
Accession Version NM_001110354.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1275bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product zona pellucida sperm-binding protein 3 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M60504.1, DB328313.1 and BC113949.1. On Nov 17, 2007 this sequence version replaced XM_001130779.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M60504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGCTGA GCTATAGGCT CTTCATCTGC CTCCTGCTCT GGGGTAGTAC TGAGCTGTGC 
TACCCCCAAC CCCTCTGGCT CTTGCAGGGT GGAGCCAGCC ATCCTGAGAC GTCCGTACAG
CCCGTACTGG TGGAGTGTCA GGAGGCCACT CTGATGGTCA TGGTCAGCAA AGACCTTTTT
GGCACCGGGA AGCTCATCAG GGCTGCTGAC CTCACCTTGG GCCCAGAGGC CTGTGAGCCT
CTGGTCTCCA TGGACACAGA AGATGTGGTC AGGTTTGAGG TTGGACTCCA CGAGTGTGGC
AACAGCATGC AGGTAACTGA CGATGCCCTG GTGTACAGCA CCTTCCTGCT CCATGACCCC
CGCCCCGTGG GAAACCTGTC CATCGTGAGG ACTAACCGCG CAGAGATTCC CATCGAGTGC
CGCTACCCCA GGCAGGGCAA TGTGAGCAGC CAGGCCATCC TGCCCACCTG GTTGCCCTTC
AGGACCACGG TGTTCTCAGA GGAGAAGCTG ACTTTCTCTC TGCGTCTGAT GGAGGAGAAC
TGGAACGCTG AGAAGAGGTC CCCCACCTTC CACCTGGGAG ATGCAGCCCA CCTCCAGGCA
GAAATCCACA CTGGCAGCCA CGTGCCACTG CGGTTGTTTG TGGACCACTG CGTGGCCACA
CCGACACCAG ACCAGAATGC CTCCCCTTAT CACACCATCG TGGACTTCCA TGGCTGTCTT
GTCGACGGTC TCACTGATGC CTCTTCTGCA TTCAAAGTTC CTCGACCCGG GCCAGATACA
CTCCAGTTCA CAGTGGATGT CTTCCACTTT GCTAATGACT CCAGAAACAT GATATACATC
ACCTGCCACC TGAAGGTCAC CCTAGCTGAG CAGGACCCAG ATGAACTCAA CAAGGCCTGT
TCCTTCAGCA AGCCTTCCAA CAGCTGGTTC CCAGTGGAAG GCTCGGCTGA CATCTGTCAA
TGCTGTAACA AAGGTGACTG TGGCACTCCA AGCCATTCCA GGAGGCAGCC TCATGTCATG
AGCCAGTGGT CCAGGTCTGC TTCCCGTAAC CGCAGGCATG TGACAGAAGA AGCAGATGTC
ACCGTGGGGC CACTGATCTT CCTGGACAGG AGGGGTGACC ATGAAGTAGA GCAGTGGGCT
TTGCCTTCTG ACACCTCAGT GGTGCTGCTG GGCGTAGGCC TGGCTGTGGT GGTGTCCCTG
ACTCTGACTG CTGTTATCCT GGTTCTCACC AGGAGGTGTC GCACTGCCTC CCACCCTGTG
TCTGCTTCCG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001103824.1
CDS11..1285
Exon (1)1..322
Translation

Target ORF information:

RefSeq Version NM_001110354.1
Organism Homo sapiens(Human)
Definition Homo sapiens zona pellucida glycoprotein 3 (ZP3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001110354.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGCTGA GCTATAGGCT CTTCATCTGC CTCCTGCTCT GGGGTAGTAC TGAGCTGTGC 
TACCCCCAAC CCCTCTGGCT CTTGCAGGGT GGAGCCAGCC ATCCTGAGAC GTCCGTACAG
CCCGTACTGG TGGAGTGTCA GGAGGCCACT CTGATGGTCA TGGTCAGCAA AGACCTTTTT
GGCACCGGGA AGCTCATCAG GGCTGCTGAC CTCACCTTGG GCCCAGAGGC CTGTGAGCCT
CTGGTCTCCA TGGACACAGA AGATGTGGTC AGGTTTGAGG TTGGACTCCA CGAGTGTGGC
AACAGCATGC AGGTAACTGA CGATGCCCTG GTGTACAGCA CCTTCCTGCT CCATGACCCC
CGCCCCGTGG GAAACCTGTC CATCGTGAGG ACTAACCGCG CAGAGATTCC CATCGAGTGC
CGCTACCCCA GGCAGGGCAA TGTGAGCAGC CAGGCCATCC TGCCCACCTG GTTGCCCTTC
AGGACCACGG TGTTCTCAGA GGAGAAGCTG ACTTTCTCTC TGCGTCTGAT GGAGGAGAAC
TGGAACGCTG AGAAGAGGTC CCCCACCTTC CACCTGGGAG ATGCAGCCCA CCTCCAGGCA
GAAATCCACA CTGGCAGCCA CGTGCCACTG CGGTTGTTTG TGGACCACTG CGTGGCCACA
CCGACACCAG ACCAGAATGC CTCCCCTTAT CACACCATCG TGGACTTCCA TGGCTGTCTT
GTCGACGGTC TCACTGATGC CTCTTCTGCA TTCAAAGTTC CTCGACCCGG GCCAGATACA
CTCCAGTTCA CAGTGGATGT CTTCCACTTT GCTAATGACT CCAGAAACAT GATATACATC
ACCTGCCACC TGAAGGTCAC CCTAGCTGAG CAGGACCCAG ATGAACTCAA CAAGGCCTGT
TCCTTCAGCA AGCCTTCCAA CAGCTGGTTC CCAGTGGAAG GCTCGGCTGA CATCTGTCAA
TGCTGTAACA AAGGTGACTG TGGCACTCCA AGCCATTCCA GGAGGCAGCC TCATGTCATG
AGCCAGTGGT CCAGGTCTGC TTCCCGTAAC CGCAGGCATG TGACAGAAGA AGCAGATGTC
ACCGTGGGGC CACTGATCTT CCTGGACAGG AGGGGTGACC ATGAAGTAGA GCAGTGGGCT
TTGCCTTCTG ACACCTCAGT GGTGCTGCTG GGCGTAGGCC TGGCTGTGGT GGTGTCCCTG
ACTCTGACTG CTGTTATCCT GGTTCTCACC AGGAGGTGTC GCACTGCCTC CCACCCTGTG
TCTGCTTCCG AATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97484
Clone ID Related Accession (Same CDS sequence) XM_017012586.1
Accession Version XM_017012586.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 681bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product zona pellucida sperm-binding protein 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016868075.1
CDS310..990
Translation

Target ORF information:

RefSeq Version XM_017012586.1
Organism Homo sapiens(Human)
Definition Homo sapiens zona pellucida glycoprotein 3 (sperm receptor) (ZP3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017012586.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGTCATGG TCAGCAAAGA CCTTTTTGGC ACCGGGAAGC TCATCAGGGC TGCTGACCTC 
ACCTTGGGCC CAGAGGCCTG TGAGCCTCTG GTCTCCATGG ACACAGAAGA TGTGGTCAGG
TTTGAGGTTG GACTCCACGA GTGTGGCAAC AGCATGCAGG TAACTGACGA TGCCCTGGTG
TACAGCACCT TCCTGCTCCA TGACCCCCGC CCCGTGGGAA ACCTGTCCAT CGTGAGGACT
AACCGCGCAG AGATTCCCAT CGAGTGCCGC TACCCCAGGC AGGGCAATGT GAGCAGCCAG
GCCATCCTGC CCACCTGGTT GCCCTTCAGG ACCACGGTGT TCTCAGAGGA GAAGCTGACT
TTCTCTCTGC GTCTGATGGA GGAGAACTGG AACGCTGAGA AGAGGTCCCC CACCTTCCAC
CTGGGAGATG CAGCCCACCT CCAGGCAGAA ATCCACACTG GCAGCCACGT GCCACTGCGG
TTGTTTGTGG ACCACTGCGT GGCCACACCG ACACCAGACC AGAATGCCTC CCCTTATCAC
ACCATCGTGG ACTTCCATGG CTGTCTTGTC GACGGTCTCA CTGATGCCTC TTCTGCATTC
AAAGTTCCTC GACCCGGGCC AGATACACTC CAGTTCACAG TGGATGTCTT CCACTTTGCT
AATGACTCCA GAAACATGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human fertilization: epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3.
Molecular human reproduction20(4)341-9(2014 Apr)
Maldera JA,Weigel Muñoz M,Chirinos M,Busso D,G E Raffo F,Battistone MA,Blaquier JA,Larrea F,Cuasnicu PS


book

Genetic and physiological study of morphologically abnormal human zona pellucida.
European journal of obstetrics, gynecology, and reproductive biology165(1)70-6(2012 Nov)
Margalit M,Paz G,Yavetz H,Yogev L,Amit A,Hevlin-Schwartz T,Gupta SK,Kleiman SE


book

Recombinant human ZP3-induced sperm acrosome reaction: evidence for the involvement of T- and L-type voltage-gated calcium channels.
Biochemical and biophysical research communications395(4)530-4(2010 May)
José O,Hernández-Hernández O,Chirinos M,González-González ME,Larrea F,Almanza A,Felix R,Darszon A,Treviño CL


book

New genetic associations detected in a host response study to hepatitis B vaccine.
Genes and immunity11(3)232-8(2010 Apr)
Davila S,Froeling FE,Tan A,Bonnard C,Boland GJ,Snippe H,Hibberd ML,Seielstad M


book

Identification of human sperm proteins that interact with human zona pellucida3 (ZP3) using yeast two-hybrid system.
Journal of reproductive immunology84(1)24-31(2010 Jan)
Naz RK,Dhandapani L


 
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