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SPAG16 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SPAG16
Entrez Gene ID 79582
Full Name sperm associated antigen 16
Synonyms PF20,WDR29
General protein information
Preferred Names

sperm associated antigen 16

Names

sperm-associated antigen 16 protein
WD repeat domain 29
pf20 protein homolog
sperm-associated WD repeat protein
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2q34
Summary Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008].
Disorder MIM:

612173


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011511821.2 XP_011510123.1 sperm-associated antigen 16 protein isoform X8
XM_011511829.1 XP_011510131.1 sperm-associated antigen 16 protein isoform X15
XM_017004899.1 XP_016860388.1 sperm-associated antigen 16 protein isoform X18
XM_011511814.1 XP_011510116.1 sperm-associated antigen 16 protein isoform X1
XM_011511824.1 XP_011510126.1 sperm-associated antigen 16 protein isoform X10
XM_011511828.1 XP_011510130.1 sperm-associated antigen 16 protein isoform X14
NM_024532.4 NP_078808.3 sperm-associated antigen 16 protein isoform 1
XM_011511822.1 XP_011510124.1 sperm-associated antigen 16 protein isoform X9
XM_017004896.1 XP_016860385.1 sperm-associated antigen 16 protein isoform X11
XM_017004897.1 XP_016860386.1 sperm-associated antigen 16 protein isoform X11
XM_011511832.1 XP_011510134.1 sperm-associated antigen 16 protein isoform X17
XM_006712746.2 XP_006712809.1 sperm-associated antigen 16 protein isoform X19
XM_017004901.1 XP_016860390.1 sperm-associated antigen 16 protein isoform X21
XM_011511837.2 XP_011510139.1 sperm-associated antigen 16 protein isoform X22
XM_011511819.2 XP_011510121.1 sperm-associated antigen 16 protein isoform X6
XM_011511817.1 XP_011510119.1 sperm-associated antigen 16 protein isoform X4
XM_011511815.1 XP_011510117.1 sperm-associated antigen 16 protein isoform X2
XM_011511818.1 XP_011510120.1 sperm-associated antigen 16 protein isoform X5
XM_011511820.1 XP_011510122.1 sperm-associated antigen 16 protein isoform X7
XM_011511823.2 XP_011510125.1 sperm-associated antigen 16 protein isoform X9
XM_017004898.1 XP_016860387.1 sperm-associated antigen 16 protein isoform X12
XM_017004902.1 XP_016860391.1 sperm-associated antigen 16 protein isoform X20
NM_001025436.2 NP_001020607.1 sperm-associated antigen 16 protein isoform 2
XM_011511816.2 XP_011510118.1 sperm-associated antigen 16 protein isoform X3
XM_011511827.1 XP_011510129.1 sperm-associated antigen 16 protein isoform X13
XM_011511831.1 XP_011510133.1 sperm-associated antigen 16 protein isoform X16
XM_011511835.2 XP_011510137.1 sperm-associated antigen 16 protein isoform X20
XM_017004900.1 XP_016860389.1 sperm-associated antigen 16 protein isoform X20

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) SPAG16 XP_536057.3
Danio rerio (zebrafish) LOC100536264 XP_003201529.2
Xenopus tropicalis (tropical clawed frog) spag16 XP_002932039.2
Rattus norvegicus (Norway rat) Spag16 NP_001128200.1
Pan troglodytes (chimpanzee) SPAG16 XP_001148592.1
LOC693900 XP_001083799.1
Bos taurus (cattle) SPAG16 XP_002685607.1
Homo sapiens (human) SPAG16 NP_078808.3
Mus musculus (house mouse) Spag16 NP_083436.2
Gallus gallus (chicken) SPAG16 XP_421865.4

Gene Ontology


Bibliography

Related articles in PubMed

Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.
Zhang L, Liu Y, Li W, Zhang Q, Li Y, Liu J, Min J, Shuang C, Song S, Zhang Z
BMC molecular biology18(1)2(2017 01)

Identification of a genetic variant for joint damage progression in autoantibody-positive rheumatoid arthritis.
Knevel R, Klein K, Somers K, Ospelt C, Houwing-Duistermaat JJ, van Nies JA, de Rooy DP, de Bock L, Kurreeman FA, Schonkeren J, Stoeken-Rijsbergen G, Helmer Q, van der Linden MP, Kern M, Manjarrez-Orduno N, Rodriguez-Rodriquez L, Stinissen P, Huizinga TW, Toes RE, Gay S, Gregersen PK, Somers V, van der Helm-van Mil AH
Annals of the rheumatic diseases73(11)2038-46(2014 Nov)

Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis.
de Bock L, Somers K, Fraussen J, Hendriks JJ, van Horssen J, Rouwette M, Hellings N, Villar LM, Alvarez-Cerme?o JC, Espi?o M, Hupperts R, Jongen P, Damoiseaux J, Verbeek MM, De Deyn PP, D'hooghe M, Van Wijmeersch B, Stinissen P, Somers V
Journal of immunology (Baltimore, Md. : 1950)193(5)2147-56(2014 Sep)

Variants in the 1q21 risk region are associated with a visual endophenotype of autism and schizophrenia.
Goodbourn PT, Bosten JM, Bargary G, Hogg RE, Lawrance-Owen AJ, Mollon JD
Genes, brain, and behavior13(2)144-51(2014 Feb)

Genome-wide contribution of genotype by environment interaction to variation of diabetes-related traits.
Zheng JS, Arnett DK, Lee YC, Shen J, Parnell LD, Smith CE, Richardson K, Li D, Borecki IB, Ordov?s JM, Lai CQ
PloS one8(10)e77442(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Short-SOX5 regulates transcription of human SPAG16L gene via directly binding to the promoter of SPAG16L.
Title: Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.

SPAG16 influences MMP-3 regulation and protects against joint destruction in autoantibody-positive rheumatoid arthritis.
Title: Identification of a genetic variant for joint damage progression in autoantibody-positive rheumatoid arthritis.

SPAG16 is a novel autoantibody target in a subgroup of MS patients and in combination with other diagnostic criteria, elevated levels of anti-SPAG16 Abs could be used as a biomarker for diagnosis.
Title: Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis.

Analysis of SPAG16 regions encoding conserved WD repeats revealed no evidence for association of mutations or genetic variation with sperm motility in infertile males.
Title: Genetic variation in SPAG16 regions encoding the WD40 repeats is not associated with reduced sperm motility and axonemal defects in a population of infertile males.

Observational study of gene-disease association. (HuGE Navigator)
Title: Multistudy fine mapping of chromosome 2q identifies XRCC5 as a chronic obstructive pulmonary disease susceptibility gene.

The following SPAG16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25048 NM_001025436.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu24849 NM_024532.4
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu36609 XM_006712746.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X21, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu71881 XM_011511814.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71882 XM_011511815.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71883 XM_011511816.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71884 XM_011511817.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71885 XM_011511818.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71886 XM_011511819.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71887 XM_011511820.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71888 XM_011511821.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71889 XM_011511823.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71889 XM_011511822.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71890 XM_011511824.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71891 XM_017004896.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71891 XM_017004897.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X13, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu71893 XM_011511827.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71894 XM_011511828.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X16, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71895 XM_011511829.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X17, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71897 XM_011511831.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71897 XM_011511832.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X19, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu71898 XM_017004900.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X22, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu71898 XM_017004902.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X25, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu71898 XM_011511835.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X24, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu71899 XM_011511837.2
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X26, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu89900 XM_017004898.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu89901 XM_017004899.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X20, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu89902 XM_017004901.1
Latest version!		 Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X23, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu25048
Clone ID Related Accession (Same CDS sequence) NM_001025436.2
Accession Version NM_001025436.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 552bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-10-03
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC025379.1, BC067756.1 and CA313710.1. On Apr 26, 2012 this sequence version replaced NM_001025436.1. Transcript Variant: This variant (2) differs in the 3' UTR and lacks a large portion of the 3' coding region, compared to variant 1. The encoded isoform (2) is significantly shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC067756.1, SRR1660805.46245.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGAGTAT 
GTTATTTTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001020607.1
CDS106..657
Misc Feature(1)58..60
Exon (1)1..241
Exon (2)242..288
Exon (3)289..384
Exon (4)385..503
Exon (5)504..1270
Translation

Target ORF information:

RefSeq Version NM_001025436.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001025436.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGAGTAT 
GTTATTTTTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24849
Clone ID Related Accession (Same CDS sequence) NM_024532.4
Accession Version NM_024532.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-10-03
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC025379.1, AF310672.1, AK302351.1 and AF490390.1. On Apr 25, 2012 this sequence version replaced NM_024532.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF490390.1, AF310672.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG GTCGAGTTTT AGCTCAGGCA 
AGTGGCAATG GTGTTATCCA TTTGCTAGAT CTTAAATCTG GGGAGATTCA CAAATTGATG 
GGCCACGAAA ACGAGGCACA CACGGTTGTG TTTTCTCACG ACGGGGAGAT TCTCTTTTCT 
GGAGGCTCTG ACGGCACAGT TCGAACGTGG TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_078808.3
CDS106..2001
Misc Feature(1)58..60
Misc Feature(2)583..918
Misc Feature(3)<1126..1998
Misc Feature(4)1135..1998
Misc Feature(5)1153..1272
Misc Feature(6)order(1156..1158,1210..1212,1222..1224,1240..1245,1279..1284,1336..1338,1348..1350,1366..1371,1405..1410,1459..1461,1474..1476,1492..1497,1534..1536,1585..1587,1600..1602,1618..1623,1657..1662,1714..1716,1726..1728,1744..1749,1783..1788,1837..1839,1852..1854,1870..1875,1906..1911,1963..1965,1975..1977,1993..1998)
Misc Feature(7)1168..1281
Misc Feature(8)1279..1398
Misc Feature(9)1297..1407
Misc Feature(10)1405..1524
Misc Feature(11)1420..1530
Misc Feature(12)1531..1650
Misc Feature(13)1549..1653
Misc Feature(14)1657..1776
Misc Feature(15)1672..1782
Misc Feature(16)1783..1905
Misc Feature(17)1798..1905
Misc Feature(18)1906..1995
Misc Feature(19)1924..1995
Exon (1)1..241
Exon (2)242..288
Exon (3)289..384
Exon (4)385..503
Exon (5)504..641
Exon (6)642..749
Exon (7)750..867
Exon (8)868..937
Exon (9)938..1047
Exon (10)1048..1175
Exon (11)1176..1319
Exon (12)1320..1505
Exon (13)1506..1632
Exon (14)1633..1698
Exon (15)1699..1825
Exon (16)1826..2187
Translation

Target ORF information:

RefSeq Version NM_024532.4
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_024532.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG GTCGAGTTTT AGCTCAGGCA 
AGTGGCAATG GTGTTATCCA TTTGCTAGAT CTTAAATCTG GGGAGATTCA CAAATTGATG 
GGCCACGAAA ACGAGGCACA CACGGTTGTG TTTTCTCACG ACGGGGAGAT TCTCTTTTCT 
GGAGGCTCTG ACGGCACAGT TCGAACGTGG TCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu36609
Clone ID Related Accession (Same CDS sequence) XM_006712746.2
Accession Version XM_006712746.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 978bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X19
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGATTATCGT ATGTGAGGTA 
GACTCACATC AGATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006712809.1
CDS57..1034
Translation

Target ORF information:

RefSeq Version XM_006712746.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X21, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006712746.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGATTATCGT ATGTGAGGTA 
GACTCACATC AGATTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71881
Clone ID Related Accession (Same CDS sequence) XM_011511814.1
Accession Version XM_011511814.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1803bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGGTGTTG GGACGAAGGC GAGGGCCTCC CACTGGCGGC GCCTTTTGAG CCTTGGGGCC 
ACTCCGGAGG AGCCTCTGTT GGACTTCAAG ATACCAGATG ACAATTTTAG CATCCCAGAA 
GGTGAAGAAG ATCTGGCAAA AGCAATTCAG ATGGCCCAAG AACAGGCTAC AGATACTGAA 
ATTTTGGAAC GGAAAACAGT TCTTCCTTCA AAGCATGCAG TACCTGAAGT AATAGAAGAC 
TTTCTCTGCA ATTTCTTGAT CAAAATGGGA ATGACCAGAA CTCTTGATTG CTTTCAGTCT 
GAATGGTATG AGTTAATACA GAAAGGAGTG ACTGAACTTA GAACTGTTGG GAATGTTCCA 
GATGTCTACA CCCAGATTAT GCTTTTGGAA AATGAGAACA AAAATTTAAA GAAAGATTTG 
AAGCACTACA AACAAGCAGC TGACAAAGCT AGAGAAGATT TGCTGAAAAT TCAGAAAGAA 
CGTGATTTTC ATCGAATGCA TCATAAGCGA ATAGTCCAGG AAAAAAACAA ATTAATTAAT 
GACCTCAAAG GGTTGAAGTT ACATTATGCA TCTTATGAAC CGACTATAAG GGTGTTACAT 
GAGAAACACC ACACTTTACT GAAGGAGAAA ATGCTGACCT CCTTGGAAAG AGACAAAGTA 
GTTGGGCAGA TTTCTGGACT TCAAGAAACA TTGAAGAAAC TGCAAAGAGG ACATAGTTAC 
CATGGTCCTC AAATTAAAGT TGATCATAGT CGTGAAAAAG AAAATGCACC AGAAGGTCCT 
ACTCAGAAAG GTCTTCGTGA AGCCAGGGAA CAAAACAAAT GTAAAACAAA GATGAAAGGC 
AATACAAAGG ATTCAGAATT TCCCATAGAT ATGCAACCAA ATCCAAACCT GAATGTTTCT 
AAAGAAAGTC TTTCTCCAGC AAAATTTGAC TACAAGCTGA AAAACATTTT TAGACTCCAT 
GAACTTCCAG TGAGCTGTGT CTCCATGCAA CCCCACAAAG ACATCCTAGT CTCCTGTGGC 
GAGGACCGAC TCTGGAAGGT GTTGGGCCTT CCAAAATGCA ATGTGCTTCT CACGGGATTT 
GGCCACACTG ACTGGCTTTC AGACTGCTGC TTCCATCCCA GTGGCGACAA ATTGGCTACT 
TCAAGTGGTG ACACTACAGT TAAATTATGG GATCTATGTA AAGGCGATTG CATTTTGACC 
TTTGAAGGAC ACAGCCGCGC AGTGTGGTCC TGCACATGGC ACTCCTGCGG CAATTTTGTG 
GCTTCCTCCT CACTGGATAA AACTAGCAAA ATTTGGGATG TTAATAGTGA AAGATGCAGA 
TGTACTTTGT ATGGACATAC AGATTCTGTG AACAGCATTG AGTTTTTTCC TTTCTCCAAT 
ACTCTTCTCA CAAGCTCTGC AGACAAGACC CTGTCTATAT GGGATGCAAG AACAGGTATA 
TGTGAGCAGT CACTTTATGG TCACATGCAT TCTATCAATG ATGCCATTTT TGATCCCAGG 
GGTCACATGA TAGCATCCTG TGATGCCTGT GGGGTTACAA AGCTGTGGGA CTTTCGGAAG 
CTGTTACCAA TTGTGTCCAT CGATATAGGT CCAAGTCCTG GCAATGAGGT GAATTTTGAT 
TCATCAGGTC GAGTTTTAGC TCAGGCAAGT GGCAATGGTG TTATCCATTT GCTAGATCTT 
AAATCTGGGG AGATTCACAA ATTGATGGGC CACGAAAACG AGGCACACAC GGTTGTGTTT 
TCTCACGACG GGGAGATTCT CTTTTCTGGA GGCTCTGACG GCACAGTTCG AACGTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510116.1
CDS247..2049
Misc Feature(1)631..966
Misc Feature(2)<1174..2046
Misc Feature(3)1183..2046
Misc Feature(4)order(1204..1206,1258..1260,1270..1272,1288..1293,1327..1332,1384..1386,1396..1398,1414..1419,1453..1458,1507..1509,1522..1524,1540..1545,1582..1584,1633..1635,1648..1650,1666..1671,1705..1710,1762..1764,1774..1776,1792..1797,1831..1836,1885..1887,1900..1902,1918..1923,1954..1959,2011..2013,2023..2025,2041..2046)
Misc Feature(5)1216..1329
Misc Feature(6)1345..1455
Misc Feature(7)1468..1578
Misc Feature(8)1597..1701
Misc Feature(9)1720..1830
Misc Feature(10)1846..1953
Misc Feature(11)1972..2043
Translation

Target ORF information:

RefSeq Version XM_011511814.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511814.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
ATGGGTGTTG GGACGAAGGC GAGGGCCTCC CACTGGCGGC GCCTTTTGAG CCTTGGGGCC 
ACTCCGGAGG AGCCTCTGTT GGACTTCAAG ATACCAGATG ACAATTTTAG CATCCCAGAA 
GGTGAAGAAG ATCTGGCAAA AGCAATTCAG ATGGCCCAAG AACAGGCTAC AGATACTGAA 
ATTTTGGAAC GGAAAACAGT TCTTCCTTCA AAGCATGCAG TACCTGAAGT AATAGAAGAC 
TTTCTCTGCA ATTTCTTGAT CAAAATGGGA ATGACCAGAA CTCTTGATTG CTTTCAGTCT 
GAATGGTATG AGTTAATACA GAAAGGAGTG ACTGAACTTA GAACTGTTGG GAATGTTCCA 
GATGTCTACA CCCAGATTAT GCTTTTGGAA AATGAGAACA AAAATTTAAA GAAAGATTTG 
AAGCACTACA AACAAGCAGC TGACAAAGCT AGAGAAGATT TGCTGAAAAT TCAGAAAGAA 
CGTGATTTTC ATCGAATGCA TCATAAGCGA ATAGTCCAGG AAAAAAACAA ATTAATTAAT 
GACCTCAAAG GGTTGAAGTT ACATTATGCA TCTTATGAAC CGACTATAAG GGTGTTACAT 
GAGAAACACC ACACTTTACT GAAGGAGAAA ATGCTGACCT CCTTGGAAAG AGACAAAGTA 
GTTGGGCAGA TTTCTGGACT TCAAGAAACA TTGAAGAAAC TGCAAAGAGG ACATAGTTAC 
CATGGTCCTC AAATTAAAGT TGATCATAGT CGTGAAAAAG AAAATGCACC AGAAGGTCCT 
ACTCAGAAAG GTCTTCGTGA AGCCAGGGAA CAAAACAAAT GTAAAACAAA GATGAAAGGC 
AATACAAAGG ATTCAGAATT TCCCATAGAT ATGCAACCAA ATCCAAACCT GAATGTTTCT 
AAAGAAAGTC TTTCTCCAGC AAAATTTGAC TACAAGCTGA AAAACATTTT TAGACTCCAT 
GAACTTCCAG TGAGCTGTGT CTCCATGCAA CCCCACAAAG ACATCCTAGT CTCCTGTGGC 
GAGGACCGAC TCTGGAAGGT GTTGGGCCTT CCAAAATGCA ATGTGCTTCT CACGGGATTT 
GGCCACACTG ACTGGCTTTC AGACTGCTGC TTCCATCCCA GTGGCGACAA ATTGGCTACT 
TCAAGTGGTG ACACTACAGT TAAATTATGG GATCTATGTA AAGGCGATTG CATTTTGACC 
TTTGAAGGAC ACAGCCGCGC AGTGTGGTCC TGCACATGGC ACTCCTGCGG CAATTTTGTG 
GCTTCCTCCT CACTGGATAA AACTAGCAAA ATTTGGGATG TTAATAGTGA AAGATGCAGA 
TGTACTTTGT ATGGACATAC AGATTCTGTG AACAGCATTG AGTTTTTTCC TTTCTCCAAT 
ACTCTTCTCA CAAGCTCTGC AGACAAGACC CTGTCTATAT GGGATGCAAG AACAGGTATA 
TGTGAGCAGT CACTTTATGG TCACATGCAT TCTATCAATG ATGCCATTTT TGATCCCAGG 
GGTCACATGA TAGCATCCTG TGATGCCTGT GGGGTTACAA AGCTGTGGGA CTTTCGGAAG 
CTGTTACCAA TTGTGTCCAT CGATATAGGT CCAAGTCCTG GCAATGAGGT GAATTTTGAT 
TCATCAGGTC GAGTTTTAGC TCAGGCAAGT GGCAATGGTG TTATCCATTT GCTAGATCTT 
AAATCTGGGG AGATTCACAA ATTGATGGGC CACGAAAACG AGGCACACAC GGTTGTGTTT 
TCTCACGACG GGGAGATTCT CTTTTCTGGA GGCTCTGACG GCACAGTTCG AACGTGGTCT 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71882
Clone ID Related Accession (Same CDS sequence) XM_011511815.1
Accession Version XM_011511815.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG AAAATACCAC GTATCTTTAT 
AGACTAGGAG CTCAGATATA TTATTTCCAG CTTTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510117.1
CDS57..1835
Misc Feature(1)534..869
Misc Feature(2)<1080..1787
Misc Feature(3)1098..1796
Misc Feature(4)1122..1232
Misc Feature(5)1245..1355
Misc Feature(6)1374..1481
Misc Feature(7)1497..1607
Misc Feature(8)1623..1703
Translation

Target ORF information:

RefSeq Version XM_011511815.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511815.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG AAAATACCAC GTATCTTTAT 
AGACTAGGAG CTCAGATATA TTATTTCCAG CTTTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71883
Clone ID Related Accession (Same CDS sequence) XM_011511816.2
Accession Version XM_011511816.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG GTCAGTGTGA TTTTAAGGCC 
CTTGGTACAT CAAACCTATT TTTTTCTCTC CATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510118.1
CDS57..1835
Misc Feature(1)534..869
Misc Feature(2)<1083..>1811
Misc Feature(3)1098..1709
Misc Feature(4)1122..1232
Misc Feature(5)1245..1355
Misc Feature(6)1374..1481
Misc Feature(7)1497..1607
Misc Feature(8)1623..1703
Translation

Target ORF information:

RefSeq Version XM_011511816.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511816.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG GTCAGTGTGA TTTTAAGGCC 
CTTGGTACAT CAAACCTATT TTTTTCTCTC CATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71884
Clone ID Related Accession (Same CDS sequence) XM_011511817.1
Accession Version XM_011511817.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1749bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG AAAAAAATGG AGATTTGTTT 
CTTCTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510119.1
CDS57..1805
Misc Feature(1)<1077..>1739
Misc Feature(2)1098..1709
Misc Feature(3)1122..1232
Misc Feature(4)1245..1355
Misc Feature(5)1374..1481
Misc Feature(6)1497..1607
Misc Feature(7)1623..1703
Translation

Target ORF information:

RefSeq Version XM_011511817.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511817.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG AAAAAAATGG AGATTTGTTT 
CTTCTATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71885
Clone ID Related Accession (Same CDS sequence) XM_011511818.1
Accession Version XM_011511818.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1734bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG TCCAGAAAGT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510120.1
CDS57..1790
Misc Feature(1)534..869
Misc Feature(2)<1077..>1739
Misc Feature(3)1098..1709
Misc Feature(4)1122..1232
Misc Feature(5)1245..1355
Misc Feature(6)1374..1481
Misc Feature(7)1497..1607
Misc Feature(8)1623..1703
Translation

Target ORF information:

RefSeq Version XM_011511818.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511818.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACAGGT ATATGTGAGC AGTCACTTTA TGGTCACATG 
CATTCTATCA ATGATGCCAT TTTTGATCCC AGGGGTCACA TGATAGCATC CTGTGATGCC 
TGTGGGGTTA CAAAGCTGTG GGACTTTCGG AAGCTGTTAC CAATTGTGTC CATCGATATA 
GGTCCAAGTC CTGGCAATGA GGTGAATTTT GATTCATCAG TCCAGAAAGT ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71886
Clone ID Related Accession (Same CDS sequence) XM_011511819.2
Accession Version XM_011511819.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1734bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGATCTTC AGAATGACAT GATACCAGAT GACAATTTTA GCATCCCAGA AGGTGAAGAA 
GATCTGGCAA AAGCAATTCA GATGGCCCAA GAACAGGCTA CAGATACTGA AATTTTGGAA 
CGGAAAACAG TTCTTCCTTC AAAGCATGCA GTACCTGAAG TAATAGAAGA CTTTCTCTGC 
AATTTCTTGA TCAAAATGGG AATGACCAGA ACTCTTGATT GCTTTCAGTC TGAATGGTAT 
GAGTTAATAC AGAAAGGAGT GACTGAACTT AGAACTGTTG GGAATGTTCC AGATGTCTAC 
ACCCAGATTA TGCTTTTGGA AAATGAGAAC AAAAATTTAA AGAAAGATTT GAAGCACTAC 
AAACAAGCAG CTGACAAAGC TAGAGAAGAT TTGCTGAAAA TTCAGAAAGA ACGTGATTTT 
CATCGAATGC ATCATAAGCG AATAGTCCAG GAAAAAAACA AATTAATTAA TGACCTCAAA 
GGGTTGAAGT TACATTATGC ATCTTATGAA CCGACTATAA GGGTGTTACA TGAGAAACAC 
CACACTTTAC TGAAGGAGAA AATGCTGACC TCCTTGGAAA GAGACAAAGT AGTTGGGCAG 
ATTTCTGGAC TTCAAGAAAC ATTGAAGAAA CTGCAAAGAG GACATAGTTA CCATGGTCCT 
CAAATTAAAG TTGATCATAG TCGTGAAAAA GAAAATGCAC CAGAAGGTCC TACTCAGAAA 
GGTCTTCGTG AAGCCAGGGA ACAAAACAAA TGTAAAACAA AGATGAAAGG CAATACAAAG 
GATTCAGAAT TTCCCATAGA TATGCAACCA AATCCAAACC TGAATGTTTC TAAAGAAAGT 
CTTTCTCCAG CAAAATTTGA CTACAAGCTG AAAAACATTT TTAGACTCCA TGAACTTCCA 
GTGAGCTGTG TCTCCATGCA ACCCCACAAA GACATCCTAG TCTCCTGTGG CGAGGACCGA 
CTCTGGAAGG TGTTGGGCCT TCCAAAATGC AATGTGCTTC TCACGGGATT TGGCCACACT 
GACTGGCTTT CAGACTGCTG CTTCCATCCC AGTGGCGACA AATTGGCTAC TTCAAGTGGT 
GACACTACAG TTAAATTATG GGATCTATGT AAAGGCGATT GCATTTTGAC CTTTGAAGGA 
CACAGCCGCG CAGTGTGGTC CTGCACATGG CACTCCTGCG GCAATTTTGT GGCTTCCTCC 
TCACTGGATA AAACTAGCAA AATTTGGGAT GTTAATAGTG AAAGATGCAG ATGTACTTTG 
TATGGACATA CAGATTCTGT GAACAGCATT GAGTTTTTTC CTTTCTCCAA TACTCTTCTC 
ACAAGCTCTG CAGACAAGAC CCTGTCTATA TGGGATGCAA GAACAGGTAT ATGTGAGCAG 
TCACTTTATG GTCACATGCA TTCTATCAAT GATGCCATTT TTGATCCCAG GGGTCACATG 
ATAGCATCCT GTGATGCCTG TGGGGTTACA AAGCTGTGGG ACTTTCGGAA GCTGTTACCA 
ATTGTGTCCA TCGATATAGG TCCAAGTCCT GGCAATGAGG TGAATTTTGA TTCATCAGGT 
CGAGTTTTAG CTCAGGCAAG TGGCAATGGT GTTATCCATT TGCTAGATCT TAAATCTGGG 
GAGATTCACA AATTGATGGG CCACGAAAAC GAGGCACACA CGGTTGTGTT TTCTCACGAC 
GGGGAGATTC TCTTTTCTGG AGGCTCTGAC GGCACAGTTC GAACGTGGTC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510121.1
CDS967..2700
Misc Feature(1)1282..1617
Misc Feature(2)<1825..2697
Misc Feature(3)1834..2697
Misc Feature(4)order(1855..1857,1909..1911,1921..1923,1939..1944,1978..1983,2035..2037,2047..2049,2065..2070,2104..2109,2158..2160,2173..2175,2191..2196,2233..2235,2284..2286,2299..2301,2317..2322,2356..2361,2413..2415,2425..2427,2443..2448,2482..2487,2536..2538,2551..2553,2569..2574,2605..2610,2662..2664,2674..2676,2692..2697)
Misc Feature(5)1867..1980
Misc Feature(6)1996..2106
Misc Feature(7)2119..2229
Misc Feature(8)2248..2352
Misc Feature(9)2371..2481
Misc Feature(10)2497..2604
Misc Feature(11)2623..2694
Translation

Target ORF information:

RefSeq Version XM_011511819.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511819.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGATCTTC AGAATGACAT GATACCAGAT GACAATTTTA GCATCCCAGA AGGTGAAGAA 
GATCTGGCAA AAGCAATTCA GATGGCCCAA GAACAGGCTA CAGATACTGA AATTTTGGAA 
CGGAAAACAG TTCTTCCTTC AAAGCATGCA GTACCTGAAG TAATAGAAGA CTTTCTCTGC 
AATTTCTTGA TCAAAATGGG AATGACCAGA ACTCTTGATT GCTTTCAGTC TGAATGGTAT 
GAGTTAATAC AGAAAGGAGT GACTGAACTT AGAACTGTTG GGAATGTTCC AGATGTCTAC 
ACCCAGATTA TGCTTTTGGA AAATGAGAAC AAAAATTTAA AGAAAGATTT GAAGCACTAC 
AAACAAGCAG CTGACAAAGC TAGAGAAGAT TTGCTGAAAA TTCAGAAAGA ACGTGATTTT 
CATCGAATGC ATCATAAGCG AATAGTCCAG GAAAAAAACA AATTAATTAA TGACCTCAAA 
GGGTTGAAGT TACATTATGC ATCTTATGAA CCGACTATAA GGGTGTTACA TGAGAAACAC 
CACACTTTAC TGAAGGAGAA AATGCTGACC TCCTTGGAAA GAGACAAAGT AGTTGGGCAG 
ATTTCTGGAC TTCAAGAAAC ATTGAAGAAA CTGCAAAGAG GACATAGTTA CCATGGTCCT 
CAAATTAAAG TTGATCATAG TCGTGAAAAA GAAAATGCAC CAGAAGGTCC TACTCAGAAA 
GGTCTTCGTG AAGCCAGGGA ACAAAACAAA TGTAAAACAA AGATGAAAGG CAATACAAAG 
GATTCAGAAT TTCCCATAGA TATGCAACCA AATCCAAACC TGAATGTTTC TAAAGAAAGT 
CTTTCTCCAG CAAAATTTGA CTACAAGCTG AAAAACATTT TTAGACTCCA TGAACTTCCA 
GTGAGCTGTG TCTCCATGCA ACCCCACAAA GACATCCTAG TCTCCTGTGG CGAGGACCGA 
CTCTGGAAGG TGTTGGGCCT TCCAAAATGC AATGTGCTTC TCACGGGATT TGGCCACACT 
GACTGGCTTT CAGACTGCTG CTTCCATCCC AGTGGCGACA AATTGGCTAC TTCAAGTGGT 
GACACTACAG TTAAATTATG GGATCTATGT AAAGGCGATT GCATTTTGAC CTTTGAAGGA 
CACAGCCGCG CAGTGTGGTC CTGCACATGG CACTCCTGCG GCAATTTTGT GGCTTCCTCC 
TCACTGGATA AAACTAGCAA AATTTGGGAT GTTAATAGTG AAAGATGCAG ATGTACTTTG 
TATGGACATA CAGATTCTGT GAACAGCATT GAGTTTTTTC CTTTCTCCAA TACTCTTCTC 
ACAAGCTCTG CAGACAAGAC CCTGTCTATA TGGGATGCAA GAACAGGTAT ATGTGAGCAG 
TCACTTTATG GTCACATGCA TTCTATCAAT GATGCCATTT TTGATCCCAG GGGTCACATG 
ATAGCATCCT GTGATGCCTG TGGGGTTACA AAGCTGTGGG ACTTTCGGAA GCTGTTACCA 
ATTGTGTCCA TCGATATAGG TCCAAGTCCT GGCAATGAGG TGAATTTTGA TTCATCAGGT 
CGAGTTTTAG CTCAGGCAAG TGGCAATGGT GTTATCCATT TGCTAGATCT TAAATCTGGG 
GAGATTCACA AATTGATGGG CCACGAAAAC GAGGCACACA CGGTTGTGTT TTCTCACGAC 
GGGGAGATTC TCTTTTCTGG AGGCTCTGAC GGCACAGTTC GAACGTGGTC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71887
Clone ID Related Accession (Same CDS sequence) XM_011511820.1
Accession Version XM_011511820.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1710bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X7
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGAAAG ATGCAGATGT ACTTTGTATG GACATACAGA TTCTGTGAAC 
AGCATTGAGT TTTTTCCTTT CTCCAATACT CTTCTCACAA GCTCTGCAGA CAAGACCCTG 
TCTATATGGG ATGCAAGAAC AGGTATATGT GAGCAGTCAC TTTATGGTCA CATGCATTCT 
ATCAATGATG CCATTTTTGA TCCCAGGGGT CACATGATAG CATCCTGTGA TGCCTGTGGG 
GTTACAAAGC TGTGGGACTT TCGGAAGCTG TTACCAATTG TGTCCATCGA TATAGGTCCA 
AGTCCTGGCA ATGAGGTGAA TTTTGATTCA TCAGGTCGAG TTTTAGCTCA GGCAAGTGGC 
AATGGTGTTA TCCATTTGCT AGATCTTAAA TCTGGGGAGA TTCACAAATT GATGGGCCAC 
GAAAACGAGG CACACACGGT TGTGTTTTCT CACGACGGGG AGATTCTCTT TTCTGGAGGC 
TCTGACGGCA CAGTTCGAAC GTGGTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510122.1
CDS57..1766
Misc Feature(1)534..869
Misc Feature(2)<960..1763
Misc Feature(3)1086..1763
Misc Feature(4)1119..1232
Misc Feature(5)1311..1421
Misc Feature(6)1440..1547
Misc Feature(7)1563..1670
Translation

Target ORF information:

RefSeq Version XM_011511820.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511820.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGAAAG ATGCAGATGT ACTTTGTATG GACATACAGA TTCTGTGAAC 
AGCATTGAGT TTTTTCCTTT CTCCAATACT CTTCTCACAA GCTCTGCAGA CAAGACCCTG 
TCTATATGGG ATGCAAGAAC AGGTATATGT GAGCAGTCAC TTTATGGTCA CATGCATTCT 
ATCAATGATG CCATTTTTGA TCCCAGGGGT CACATGATAG CATCCTGTGA TGCCTGTGGG 
GTTACAAAGC TGTGGGACTT TCGGAAGCTG TTACCAATTG TGTCCATCGA TATAGGTCCA 
AGTCCTGGCA ATGAGGTGAA TTTTGATTCA TCAGGTCGAG TTTTAGCTCA GGCAAGTGGC 
AATGGTGTTA TCCATTTGCT AGATCTTAAA TCTGGGGAGA TTCACAAATT GATGGGCCAC 
GAAAACGAGG CACACACGGT TGTGTTTTCT CACGACGGGG AGATTCTCTT TTCTGGAGGC 
TCTGACGGCA CAGTTCGAAC GTGGTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71888
Clone ID Related Accession (Same CDS sequence) XM_011511821.2
Accession Version XM_011511821.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1614bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X8
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGACTATGA ATATGAAGAG ATACCAGATG ACAATTTTAG CATCCCAGAA GGTGAAGAAG 
ATCTGGCAAA AGCAATTCAG ATGGCCCAAG AACAGGCTAC AGATACTGAA ATTTTGGTAT 
GAGTTAATAC AGAAAGGAGT GACTGAACTT AGAACTGTTG GGAATGTTCC AGATGTCTAC 
ACCCAGATTA TGCTTTTGGA AAATGAGAAC AAAAATTTAA AGAAAGATTT GAAGCACTAC 
AAACAAGCAG CTGACAAAGC TAGAGAAGAT TTGCTGAAAA TTCAGAAAGA ACGTGATTTT 
CATCGAATGC ATCATAAGCG AATAGTCCAG GAAAAAAACA AATTAATTAA TGACCTCAAA 
GGGTTGAAGT TACATTATGC ATCTTATGAA CCGACTATAA GGGTGTTACA TGAGAAACAC 
CACACTTTAC TGAAGGAGAA AATGCTGACC TCCTTGGAAA GAGACAAAGT AGTTGGGCAG 
ATTTCTGGAC TTCAAGAAAC ATTGAAGAAA CTGCAAAGAG GACATAGTTA CCATGGTCCT 
CAAATTAAAG TTGATCATAG TCGTGAAAAA GAAAATGCAC CAGAAGGTCC TACTCAGAAA 
GGTCTTCGTG AAGCCAGGGA ACAAAACAAA TGTAAAACAA AGATGAAAGG CAATACAAAG 
GATTCAGAAT TTCCCATAGA TATGCAACCA AATCCAAACC TGAATGTTTC TAAAGAAAGT 
CTTTCTCCAG CAAAATTTGA CTACAAGCTG AAAAACATTT TTAGACTCCA TGAACTTCCA 
GTGAGCTGTG TCTCCATGCA ACCCCACAAA GACATCCTAG TCTCCTGTGG CGAGGACCGA 
CTCTGGAAGG TGTTGGGCCT TCCAAAATGC AATGTGCTTC TCACGGGATT TGGCCACACT 
GACTGGCTTT CAGACTGCTG CTTCCATCCC AGTGGCGACA AATTGGCTAC TTCAAGTGGT 
GACACTACAG TTAAATTATG GGATCTATGT AAAGGCGATT GCATTTTGAC CTTTGAAGGA 
CACAGCCGCG CAGTGTGGTC CTGCACATGG CACTCCTGCG GCAATTTTGT GGCTTCCTCC 
TCACTGGATA AAACTAGCAA AATTTGGGAT GTTAATAGTG AAAGATGCAG ATGTACTTTG 
TATGGACATA CAGATTCTGT GAACAGCATT GAGTTTTTTC CTTTCTCCAA TACTCTTCTC 
ACAAGCTCTG CAGACAAGAC CCTGTCTATA TGGGATGCAA GAACAGGTAT ATGTGAGCAG 
TCACTTTATG GTCACATGCA TTCTATCAAT GATGCCATTT TTGATCCCAG GGGTCACATG 
ATAGCATCCT GTGATGCCTG TGGGGTTACA AAGCTGTGGG ACTTTCGGAA GCTGTTACCA 
ATTGTGTCCA TCGATATAGG TCCAAGTCCT GGCAATGAGG TGAATTTTGA TTCATCAGGT 
CGAGTTTTAG CTCAGGCAAG TGGCAATGGT GTTATCCATT TGCTAGATCT TAAATCTGGG 
GAGATTCACA AATTGATGGG CCACGAAAAC GAGGCACACA CGGTTGTGTT TTCTCACGAC 
GGGGAGATTC TCTTTTCTGG AGGCTCTGAC GGCACAGTTC GAACGTGGTC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510123.1
CDS1213..2826
Misc Feature(1)1408..1743
Misc Feature(2)<1951..2823
Misc Feature(3)1960..2823
Misc Feature(4)order(1981..1983,2035..2037,2047..2049,2065..2070,2104..2109,2161..2163,2173..2175,2191..2196,2230..2235,2284..2286,2299..2301,2317..2322,2359..2361,2410..2412,2425..2427,2443..2448,2482..2487,2539..2541,2551..2553,2569..2574,2608..2613,2662..2664,2677..2679,2695..2700,2731..2736,2788..2790,2800..2802,2818..2823)
Misc Feature(5)1993..2106
Misc Feature(6)2122..2232
Misc Feature(7)2245..2355
Misc Feature(8)2374..2478
Misc Feature(9)2497..2607
Misc Feature(10)2623..2730
Misc Feature(11)2749..2820
Translation

Target ORF information:

RefSeq Version XM_011511821.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511821.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGACTATGA ATATGAAGAG ATACCAGATG ACAATTTTAG CATCCCAGAA GGTGAAGAAG 
ATCTGGCAAA AGCAATTCAG ATGGCCCAAG AACAGGCTAC AGATACTGAA ATTTTGGTAT 
GAGTTAATAC AGAAAGGAGT GACTGAACTT AGAACTGTTG GGAATGTTCC AGATGTCTAC 
ACCCAGATTA TGCTTTTGGA AAATGAGAAC AAAAATTTAA AGAAAGATTT GAAGCACTAC 
AAACAAGCAG CTGACAAAGC TAGAGAAGAT TTGCTGAAAA TTCAGAAAGA ACGTGATTTT 
CATCGAATGC ATCATAAGCG AATAGTCCAG GAAAAAAACA AATTAATTAA TGACCTCAAA 
GGGTTGAAGT TACATTATGC ATCTTATGAA CCGACTATAA GGGTGTTACA TGAGAAACAC 
CACACTTTAC TGAAGGAGAA AATGCTGACC TCCTTGGAAA GAGACAAAGT AGTTGGGCAG 
ATTTCTGGAC TTCAAGAAAC ATTGAAGAAA CTGCAAAGAG GACATAGTTA CCATGGTCCT 
CAAATTAAAG TTGATCATAG TCGTGAAAAA GAAAATGCAC CAGAAGGTCC TACTCAGAAA 
GGTCTTCGTG AAGCCAGGGA ACAAAACAAA TGTAAAACAA AGATGAAAGG CAATACAAAG 
GATTCAGAAT TTCCCATAGA TATGCAACCA AATCCAAACC TGAATGTTTC TAAAGAAAGT 
CTTTCTCCAG CAAAATTTGA CTACAAGCTG AAAAACATTT TTAGACTCCA TGAACTTCCA 
GTGAGCTGTG TCTCCATGCA ACCCCACAAA GACATCCTAG TCTCCTGTGG CGAGGACCGA 
CTCTGGAAGG TGTTGGGCCT TCCAAAATGC AATGTGCTTC TCACGGGATT TGGCCACACT 
GACTGGCTTT CAGACTGCTG CTTCCATCCC AGTGGCGACA AATTGGCTAC TTCAAGTGGT 
GACACTACAG TTAAATTATG GGATCTATGT AAAGGCGATT GCATTTTGAC CTTTGAAGGA 
CACAGCCGCG CAGTGTGGTC CTGCACATGG CACTCCTGCG GCAATTTTGT GGCTTCCTCC 
TCACTGGATA AAACTAGCAA AATTTGGGAT GTTAATAGTG AAAGATGCAG ATGTACTTTG 
TATGGACATA CAGATTCTGT GAACAGCATT GAGTTTTTTC CTTTCTCCAA TACTCTTCTC 
ACAAGCTCTG CAGACAAGAC CCTGTCTATA TGGGATGCAA GAACAGGTAT ATGTGAGCAG 
TCACTTTATG GTCACATGCA TTCTATCAAT GATGCCATTT TTGATCCCAG GGGTCACATG 
ATAGCATCCT GTGATGCCTG TGGGGTTACA AAGCTGTGGG ACTTTCGGAA GCTGTTACCA 
ATTGTGTCCA TCGATATAGG TCCAAGTCCT GGCAATGAGG TGAATTTTGA TTCATCAGGT 
CGAGTTTTAG CTCAGGCAAG TGGCAATGGT GTTATCCATT TGCTAGATCT TAAATCTGGG 
GAGATTCACA AATTGATGGG CCACGAAAAC GAGGCACACA CGGTTGTGTT TTCTCACGAC 
GGGGAGATTC TCTTTTCTGG AGGCTCTGAC GGCACAGTTC GAACGTGGTC TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71889
Clone ID Related Accession (Same CDS sequence) XM_011511822.1 , XM_011511823.2
Accession Version XM_011511822.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1587bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X9
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT CAGATGGCCC 
AAGAACAGGC TACAGATACT GAAATTTTGG TATGAGTTAA TACAGAAAGG AGTGACTGAA 
CTTAGAACTG TTGGGAATGT TCCAGATGTC TACACCCAGA TTATGCTTTT GGAAAATGAG 
AACAAAAATT TAAAGAAAGA TTTGAAGCAC TACAAACAAG CAGCTGACAA AGCTAGAGAA 
GATTTGCTGA AAATTCAGAA AGAACGTGAT TTTCATCGAA TGCATCATAA GCGAATAGTC 
CAGGAAAAAA ACAAATTAAT TAATGACCTC AAAGGGTTGA AGTTACATTA TGCATCTTAT 
GAACCGACTA TAAGGGTGTT ACATGAGAAA CACCACACTT TACTGAAGGA GAAAATGCTG 
ACCTCCTTGG AAAGAGACAA AGTAGTTGGG CAGATTTCTG GACTTCAAGA AACATTGAAG 
AAACTGCAAA GAGGACATAG TTACCATGGT CCTCAAATTA AAGTTGATCA TAGTCGTGAA 
AAAGAAAATG CACCAGAAGG TCCTACTCAG AAAGGTCTTC GTGAAGCCAG GGAACAAAAC 
AAATGTAAAA CAAAGATGAA AGGCAATACA AAGGATTCAG AATTTCCCAT AGATATGCAA 
CCAAATCCAA ACCTGAATGT TTCTAAAGAA AGTCTTTCTC CAGCAAAATT TGACTACAAG 
CTGAAAAACA TTTTTAGACT CCATGAACTT CCAGTGAGCT GTGTCTCCAT GCAACCCCAC 
AAAGACATCC TAGTCTCCTG TGGCGAGGAC CGACTCTGGA AGGTGTTGGG CCTTCCAAAA 
TGCAATGTGC TTCTCACGGG ATTTGGCCAC ACTGACTGGC TTTCAGACTG CTGCTTCCAT 
CCCAGTGGCG ACAAATTGGC TACTTCAAGT GGTGACACTA CAGTTAAATT ATGGGATCTA 
TGTAAAGGCG ATTGCATTTT GACCTTTGAA GGACACAGCC GCGCAGTGTG GTCCTGCACA 
TGGCACTCCT GCGGCAATTT TGTGGCTTCC TCCTCACTGG ATAAAACTAG CAAAATTTGG 
GATGTTAATA GTGAAAGATG CAGATGTACT TTGTATGGAC ATACAGATTC TGTGAACAGC 
ATTGAGTTTT TTCCTTTCTC CAATACTCTT CTCACAAGCT CTGCAGACAA GACCCTGTCT 
ATATGGGATG CAAGAACAGG TATATGTGAG CAGTCACTTT ATGGTCACAT GCATTCTATC 
AATGATGCCA TTTTTGATCC CAGGGGTCAC ATGATAGCAT CCTGTGATGC CTGTGGGGTT 
ACAAAGCTGT GGGACTTTCG GAAGCTGTTA CCAATTGTGT CCATCGATAT AGGTCCAAGT 
CCTGGCAATG AGGTGAATTT TGATTCATCA GGTCGAGTTT TAGCTCAGGC AAGTGGCAAT 
GGTGTTATCC ATTTGCTAGA TCTTAAATCT GGGGAGATTC ACAAATTGAT GGGCCACGAA 
AACGAGGCAC ACACGGTTGT GTTTTCTCAC GACGGGGAGA TTCTCTTTTC TGGAGGCTCT 
GACGGCACAG TTCGAACGTG GTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510124.1
CDS96..1682
Misc Feature(1)264..599
Misc Feature(2)<807..1679
Misc Feature(3)816..1679
Misc Feature(4)order(837..839,891..893,903..905,921..926,960..965,1017..1019,1029..1031,1047..1052,1086..1091,1140..1142,1155..1157,1173..1178,1215..1217,1266..1268,1281..1283,1299..1304,1338..1343,1395..1397,1407..1409,1425..1430,1464..1469,1518..1520,1533..1535,1551..1556,1587..1592,1644..1646,1656..1658,1674..1679)
Misc Feature(5)849..962
Misc Feature(6)978..1088
Misc Feature(7)1101..1211
Misc Feature(8)1230..1334
Misc Feature(9)1353..1463
Misc Feature(10)1479..1586
Misc Feature(11)1605..1676
Translation

Target ORF information:

RefSeq Version XM_011511822.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511822.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT CAGATGGCCC 
AAGAACAGGC TACAGATACT GAAATTTTGG TATGAGTTAA TACAGAAAGG AGTGACTGAA 
CTTAGAACTG TTGGGAATGT TCCAGATGTC TACACCCAGA TTATGCTTTT GGAAAATGAG 
AACAAAAATT TAAAGAAAGA TTTGAAGCAC TACAAACAAG CAGCTGACAA AGCTAGAGAA 
GATTTGCTGA AAATTCAGAA AGAACGTGAT TTTCATCGAA TGCATCATAA GCGAATAGTC 
CAGGAAAAAA ACAAATTAAT TAATGACCTC AAAGGGTTGA AGTTACATTA TGCATCTTAT 
GAACCGACTA TAAGGGTGTT ACATGAGAAA CACCACACTT TACTGAAGGA GAAAATGCTG 
ACCTCCTTGG AAAGAGACAA AGTAGTTGGG CAGATTTCTG GACTTCAAGA AACATTGAAG 
AAACTGCAAA GAGGACATAG TTACCATGGT CCTCAAATTA AAGTTGATCA TAGTCGTGAA 
AAAGAAAATG CACCAGAAGG TCCTACTCAG AAAGGTCTTC GTGAAGCCAG GGAACAAAAC 
AAATGTAAAA CAAAGATGAA AGGCAATACA AAGGATTCAG AATTTCCCAT AGATATGCAA 
CCAAATCCAA ACCTGAATGT TTCTAAAGAA AGTCTTTCTC CAGCAAAATT TGACTACAAG 
CTGAAAAACA TTTTTAGACT CCATGAACTT CCAGTGAGCT GTGTCTCCAT GCAACCCCAC 
AAAGACATCC TAGTCTCCTG TGGCGAGGAC CGACTCTGGA AGGTGTTGGG CCTTCCAAAA 
TGCAATGTGC TTCTCACGGG ATTTGGCCAC ACTGACTGGC TTTCAGACTG CTGCTTCCAT 
CCCAGTGGCG ACAAATTGGC TACTTCAAGT GGTGACACTA CAGTTAAATT ATGGGATCTA 
TGTAAAGGCG ATTGCATTTT GACCTTTGAA GGACACAGCC GCGCAGTGTG GTCCTGCACA 
TGGCACTCCT GCGGCAATTT TGTGGCTTCC TCCTCACTGG ATAAAACTAG CAAAATTTGG 
GATGTTAATA GTGAAAGATG CAGATGTACT TTGTATGGAC ATACAGATTC TGTGAACAGC 
ATTGAGTTTT TTCCTTTCTC CAATACTCTT CTCACAAGCT CTGCAGACAA GACCCTGTCT 
ATATGGGATG CAAGAACAGG TATATGTGAG CAGTCACTTT ATGGTCACAT GCATTCTATC 
AATGATGCCA TTTTTGATCC CAGGGGTCAC ATGATAGCAT CCTGTGATGC CTGTGGGGTT 
ACAAAGCTGT GGGACTTTCG GAAGCTGTTA CCAATTGTGT CCATCGATAT AGGTCCAAGT 
CCTGGCAATG AGGTGAATTT TGATTCATCA GGTCGAGTTT TAGCTCAGGC AAGTGGCAAT 
GGTGTTATCC ATTTGCTAGA TCTTAAATCT GGGGAGATTC ACAAATTGAT GGGCCACGAA 
AACGAGGCAC ACACGGTTGT GTTTTCTCAC GACGGGGAGA TTCTCTTTTC TGGAGGCTCT 
GACGGCACAG TTCGAACGTG GTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71889
Clone ID Related Accession (Same CDS sequence) XM_011511822.1 , XM_011511823.2
Accession Version XM_011511823.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1587bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X9
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT CAGATGGCCC 
AAGAACAGGC TACAGATACT GAAATTTTGG TATGAGTTAA TACAGAAAGG AGTGACTGAA 
CTTAGAACTG TTGGGAATGT TCCAGATGTC TACACCCAGA TTATGCTTTT GGAAAATGAG 
AACAAAAATT TAAAGAAAGA TTTGAAGCAC TACAAACAAG CAGCTGACAA AGCTAGAGAA 
GATTTGCTGA AAATTCAGAA AGAACGTGAT TTTCATCGAA TGCATCATAA GCGAATAGTC 
CAGGAAAAAA ACAAATTAAT TAATGACCTC AAAGGGTTGA AGTTACATTA TGCATCTTAT 
GAACCGACTA TAAGGGTGTT ACATGAGAAA CACCACACTT TACTGAAGGA GAAAATGCTG 
ACCTCCTTGG AAAGAGACAA AGTAGTTGGG CAGATTTCTG GACTTCAAGA AACATTGAAG 
AAACTGCAAA GAGGACATAG TTACCATGGT CCTCAAATTA AAGTTGATCA TAGTCGTGAA 
AAAGAAAATG CACCAGAAGG TCCTACTCAG AAAGGTCTTC GTGAAGCCAG GGAACAAAAC 
AAATGTAAAA CAAAGATGAA AGGCAATACA AAGGATTCAG AATTTCCCAT AGATATGCAA 
CCAAATCCAA ACCTGAATGT TTCTAAAGAA AGTCTTTCTC CAGCAAAATT TGACTACAAG 
CTGAAAAACA TTTTTAGACT CCATGAACTT CCAGTGAGCT GTGTCTCCAT GCAACCCCAC 
AAAGACATCC TAGTCTCCTG TGGCGAGGAC CGACTCTGGA AGGTGTTGGG CCTTCCAAAA 
TGCAATGTGC TTCTCACGGG ATTTGGCCAC ACTGACTGGC TTTCAGACTG CTGCTTCCAT 
CCCAGTGGCG ACAAATTGGC TACTTCAAGT GGTGACACTA CAGTTAAATT ATGGGATCTA 
TGTAAAGGCG ATTGCATTTT GACCTTTGAA GGACACAGCC GCGCAGTGTG GTCCTGCACA 
TGGCACTCCT GCGGCAATTT TGTGGCTTCC TCCTCACTGG ATAAAACTAG CAAAATTTGG 
GATGTTAATA GTGAAAGATG CAGATGTACT TTGTATGGAC ATACAGATTC TGTGAACAGC 
ATTGAGTTTT TTCCTTTCTC CAATACTCTT CTCACAAGCT CTGCAGACAA GACCCTGTCT 
ATATGGGATG CAAGAACAGG TATATGTGAG CAGTCACTTT ATGGTCACAT GCATTCTATC 
AATGATGCCA TTTTTGATCC CAGGGGTCAC ATGATAGCAT CCTGTGATGC CTGTGGGGTT 
ACAAAGCTGT GGGACTTTCG GAAGCTGTTA CCAATTGTGT CCATCGATAT AGGTCCAAGT 
CCTGGCAATG AGGTGAATTT TGATTCATCA GGTCGAGTTT TAGCTCAGGC AAGTGGCAAT 
GGTGTTATCC ATTTGCTAGA TCTTAAATCT GGGGAGATTC ACAAATTGAT GGGCCACGAA 
AACGAGGCAC ACACGGTTGT GTTTTCTCAC GACGGGGAGA TTCTCTTTTC TGGAGGCTCT 
GACGGCACAG TTCGAACGTG GTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510125.1
CDS1018..2604
Misc Feature(1)1186..1521
Misc Feature(2)<1729..2601
Misc Feature(3)1738..2601
Misc Feature(4)order(1759..1761,1813..1815,1825..1827,1843..1848,1882..1887,1939..1941,1951..1953,1969..1974,2008..2013,2062..2064,2077..2079,2095..2100,2137..2139,2188..2190,2203..2205,2221..2226,2260..2265,2317..2319,2329..2331,2347..2352,2386..2391,2440..2442,2455..2457,2473..2478,2509..2514,2566..2568,2578..2580,2596..2601)
Misc Feature(5)1771..1884
Misc Feature(6)1900..2010
Misc Feature(7)2023..2133
Misc Feature(8)2152..2256
Misc Feature(9)2275..2385
Misc Feature(10)2401..2508
Misc Feature(11)2527..2598
Translation

Target ORF information:

RefSeq Version XM_011511823.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511823.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT CAGATGGCCC 
AAGAACAGGC TACAGATACT GAAATTTTGG TATGAGTTAA TACAGAAAGG AGTGACTGAA 
CTTAGAACTG TTGGGAATGT TCCAGATGTC TACACCCAGA TTATGCTTTT GGAAAATGAG 
AACAAAAATT TAAAGAAAGA TTTGAAGCAC TACAAACAAG CAGCTGACAA AGCTAGAGAA 
GATTTGCTGA AAATTCAGAA AGAACGTGAT TTTCATCGAA TGCATCATAA GCGAATAGTC 
CAGGAAAAAA ACAAATTAAT TAATGACCTC AAAGGGTTGA AGTTACATTA TGCATCTTAT 
GAACCGACTA TAAGGGTGTT ACATGAGAAA CACCACACTT TACTGAAGGA GAAAATGCTG 
ACCTCCTTGG AAAGAGACAA AGTAGTTGGG CAGATTTCTG GACTTCAAGA AACATTGAAG 
AAACTGCAAA GAGGACATAG TTACCATGGT CCTCAAATTA AAGTTGATCA TAGTCGTGAA 
AAAGAAAATG CACCAGAAGG TCCTACTCAG AAAGGTCTTC GTGAAGCCAG GGAACAAAAC 
AAATGTAAAA CAAAGATGAA AGGCAATACA AAGGATTCAG AATTTCCCAT AGATATGCAA 
CCAAATCCAA ACCTGAATGT TTCTAAAGAA AGTCTTTCTC CAGCAAAATT TGACTACAAG 
CTGAAAAACA TTTTTAGACT CCATGAACTT CCAGTGAGCT GTGTCTCCAT GCAACCCCAC 
AAAGACATCC TAGTCTCCTG TGGCGAGGAC CGACTCTGGA AGGTGTTGGG CCTTCCAAAA 
TGCAATGTGC TTCTCACGGG ATTTGGCCAC ACTGACTGGC TTTCAGACTG CTGCTTCCAT 
CCCAGTGGCG ACAAATTGGC TACTTCAAGT GGTGACACTA CAGTTAAATT ATGGGATCTA 
TGTAAAGGCG ATTGCATTTT GACCTTTGAA GGACACAGCC GCGCAGTGTG GTCCTGCACA 
TGGCACTCCT GCGGCAATTT TGTGGCTTCC TCCTCACTGG ATAAAACTAG CAAAATTTGG 
GATGTTAATA GTGAAAGATG CAGATGTACT TTGTATGGAC ATACAGATTC TGTGAACAGC 
ATTGAGTTTT TTCCTTTCTC CAATACTCTT CTCACAAGCT CTGCAGACAA GACCCTGTCT 
ATATGGGATG CAAGAACAGG TATATGTGAG CAGTCACTTT ATGGTCACAT GCATTCTATC 
AATGATGCCA TTTTTGATCC CAGGGGTCAC ATGATAGCAT CCTGTGATGC CTGTGGGGTT 
ACAAAGCTGT GGGACTTTCG GAAGCTGTTA CCAATTGTGT CCATCGATAT AGGTCCAAGT 
CCTGGCAATG AGGTGAATTT TGATTCATCA GGTCGAGTTT TAGCTCAGGC AAGTGGCAAT 
GGTGTTATCC ATTTGCTAGA TCTTAAATCT GGGGAGATTC ACAAATTGAT GGGCCACGAA 
AACGAGGCAC ACACGGTTGT GTTTTCTCAC GACGGGGAGA TTCTCTTTTC TGGAGGCTCT 
GACGGCACAG TTCGAACGTG GTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71890
Clone ID Related Accession (Same CDS sequence) XM_011511824.1
Accession Version XM_011511824.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X10
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACATCT CTGCTCTCTT ATAGTTTAAT CTCGACATGG 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510126.1
CDS57..1619
Misc Feature(1)<1077..>1604
Misc Feature(2)1098..>1610
Misc Feature(3)1122..1232
Misc Feature(4)1245..1355
Misc Feature(5)1374..1481
Translation

Target ORF information:

RefSeq Version XM_011511824.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511824.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGTCTCCATG 
CAACCCCACA AAGACATCCT AGTCTCCTGT GGCGAGGACC GACTCTGGAA GGTGTTGGGC 
CTTCCAAAAT GCAATGTGCT TCTCACGGGA TTTGGCCACA CTGACTGGCT TTCAGACTGC 
TGCTTCCATC CCAGTGGCGA CAAATTGGCT ACTTCAAGTG GTGACACTAC AGTTAAATTA 
TGGGATCTAT GTAAAGGCGA TTGCATTTTG ACCTTTGAAG GACACAGCCG CGCAGTGTGG 
TCCTGCACAT GGCACTCCTG CGGCAATTTT GTGGCTTCCT CCTCACTGGA TAAAACTAGC 
AAAATTTGGG ATGTTAATAG TGAAAGATGC AGATGTACTT TGTATGGACA TACAGATTCT 
GTGAACAGCA TTGAGTTTTT TCCTTTCTCC AATACTCTTC TCACAAGCTC TGCAGACAAG 
ACCCTGTCTA TATGGGATGC AAGAACATCT CTGCTCTCTT ATAGTTTAAT CTCGACATGG 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71891
Clone ID Related Accession (Same CDS sequence) XM_017004896.1 , XM_017004897.1
Accession Version XM_017004896.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1539bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X11
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGAATGA CCAGAACTCT TGATTGCTTT CAGTCTGAAT GGTATGAGTT AATACAGAAA 
GGAGTGACTG AACTTAGAAC TGTTGGGAAT GTTCCAGATG TCTACACCCA GATTATGCTT 
TTGGAAAATG AGAACAAAAA TTTAAAGAAA GATTTGAAGC ACTACAAACA AGCAGCTGAC 
AAAGCTAGAG AAGATTTGCT GAAAATTCAG AAAGAACGTG ATTTTCATCG AATGCATCAT 
AAGCGAATAG TCCAGGAAAA AAACAAATTA ATTAATGACC TCAAAGGGTT GAAGTTACAT 
TATGCATCTT ATGAACCGAC TATAAGGGTG TTACATGAGA AACACCACAC TTTACTGAAG 
GAGAAAATGC TGACCTCCTT GGAAAGAGAC AAAGTAGTTG GGCAGATTTC TGGACTTCAA 
GAAACATTGA AGAAACTGCA AAGAGGACAT AGTTACCATG GTCCTCAAAT TAAAGTTGAT 
CATAGTCGTG AAAAAGAAAA TGCACCAGAA GGTCCTACTC AGAAAGGTCT TCGTGAAGCC 
AGGGAACAAA ACAAATGTAA AACAAAGATG AAAGGCAATA CAAAGGATTC AGAATTTCCC 
ATAGATATGC AACCAAATCC AAACCTGAAT GTTTCTAAAG AAAGTCTTTC TCCAGCAAAA 
TTTGACTACA AGCTGAAAAA CATTTTTAGA CTCCATGAAC TTCCAGTGAG CTGTGTCTCC 
ATGCAACCCC ACAAAGACAT CCTAGTCTCC TGTGGCGAGG ACCGACTCTG GAAGGTGTTG 
GGCCTTCCAA AATGCAATGT GCTTCTCACG GGATTTGGCC ACACTGACTG GCTTTCAGAC 
TGCTGCTTCC ATCCCAGTGG CGACAAATTG GCTACTTCAA GTGGTGACAC TACAGTTAAA 
TTATGGGATC TATGTAAAGG CGATTGCATT TTGACCTTTG AAGGACACAG CCGCGCAGTG 
TGGTCCTGCA CATGGCACTC CTGCGGCAAT TTTGTGGCTT CCTCCTCACT GGATAAAACT 
AGCAAAATTT GGGATGTTAA TAGTGAAAGA TGCAGATGTA CTTTGTATGG ACATACAGAT 
TCTGTGAACA GCATTGAGTT TTTTCCTTTC TCCAATACTC TTCTCACAAG CTCTGCAGAC 
AAGACCCTGT CTATATGGGA TGCAAGAACA GGTATATGTG AGCAGTCACT TTATGGTCAC 
ATGCATTCTA TCAATGATGC CATTTTTGAT CCCAGGGGTC ACATGATAGC ATCCTGTGAT 
GCCTGTGGGG TTACAAAGCT GTGGGACTTT CGGAAGCTGT TACCAATTGT GTCCATCGAT 
ATAGGTCCAA GTCCTGGCAA TGAGGTGAAT TTTGATTCAT CAGGTCGAGT TTTAGCTCAG 
GCAAGTGGCA ATGGTGTTAT CCATTTGCTA GATCTTAAAT CTGGGGAGAT TCACAAATTG 
ATGGGCCACG AAAACGAGGC ACACACGGTT GTGTTTTCTC ACGACGGGGA GATTCTCTTT 
TCTGGAGGCT CTGACGGCAC AGTTCGAACG TGGTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860385.1
CDS543..2081
Misc Feature(1)663..998
Misc Feature(2)<1206..2078
Misc Feature(3)1215..2078
Misc Feature(4)order(1236..1238,1290..1292,1302..1304,1320..1325,1359..1364,1416..1418,1428..1430,1446..1451,1485..1490,1539..1541,1554..1556,1572..1577,1614..1616,1665..1667,1680..1682,1698..1703,1737..1742,1794..1796,1806..1808,1824..1829,1863..1868,1917..1919,1932..1934,1950..1955,1986..1991,2043..2045,2055..2057,2073..2078)
Misc Feature(5)1248..1361
Misc Feature(6)1377..1487
Misc Feature(7)1500..1610
Misc Feature(8)1629..1733
Misc Feature(9)1752..1862
Misc Feature(10)1878..1985
Misc Feature(11)2004..2075
Translation

Target ORF information:

RefSeq Version XM_017004896.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004896.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGAATGA CCAGAACTCT TGATTGCTTT CAGTCTGAAT GGTATGAGTT AATACAGAAA 
GGAGTGACTG AACTTAGAAC TGTTGGGAAT GTTCCAGATG TCTACACCCA GATTATGCTT 
TTGGAAAATG AGAACAAAAA TTTAAAGAAA GATTTGAAGC ACTACAAACA AGCAGCTGAC 
AAAGCTAGAG AAGATTTGCT GAAAATTCAG AAAGAACGTG ATTTTCATCG AATGCATCAT 
AAGCGAATAG TCCAGGAAAA AAACAAATTA ATTAATGACC TCAAAGGGTT GAAGTTACAT 
TATGCATCTT ATGAACCGAC TATAAGGGTG TTACATGAGA AACACCACAC TTTACTGAAG 
GAGAAAATGC TGACCTCCTT GGAAAGAGAC AAAGTAGTTG GGCAGATTTC TGGACTTCAA 
GAAACATTGA AGAAACTGCA AAGAGGACAT AGTTACCATG GTCCTCAAAT TAAAGTTGAT 
CATAGTCGTG AAAAAGAAAA TGCACCAGAA GGTCCTACTC AGAAAGGTCT TCGTGAAGCC 
AGGGAACAAA ACAAATGTAA AACAAAGATG AAAGGCAATA CAAAGGATTC AGAATTTCCC 
ATAGATATGC AACCAAATCC AAACCTGAAT GTTTCTAAAG AAAGTCTTTC TCCAGCAAAA 
TTTGACTACA AGCTGAAAAA CATTTTTAGA CTCCATGAAC TTCCAGTGAG CTGTGTCTCC 
ATGCAACCCC ACAAAGACAT CCTAGTCTCC TGTGGCGAGG ACCGACTCTG GAAGGTGTTG 
GGCCTTCCAA AATGCAATGT GCTTCTCACG GGATTTGGCC ACACTGACTG GCTTTCAGAC 
TGCTGCTTCC ATCCCAGTGG CGACAAATTG GCTACTTCAA GTGGTGACAC TACAGTTAAA 
TTATGGGATC TATGTAAAGG CGATTGCATT TTGACCTTTG AAGGACACAG CCGCGCAGTG 
TGGTCCTGCA CATGGCACTC CTGCGGCAAT TTTGTGGCTT CCTCCTCACT GGATAAAACT 
AGCAAAATTT GGGATGTTAA TAGTGAAAGA TGCAGATGTA CTTTGTATGG ACATACAGAT 
TCTGTGAACA GCATTGAGTT TTTTCCTTTC TCCAATACTC TTCTCACAAG CTCTGCAGAC 
AAGACCCTGT CTATATGGGA TGCAAGAACA GGTATATGTG AGCAGTCACT TTATGGTCAC 
ATGCATTCTA TCAATGATGC CATTTTTGAT CCCAGGGGTC ACATGATAGC ATCCTGTGAT 
GCCTGTGGGG TTACAAAGCT GTGGGACTTT CGGAAGCTGT TACCAATTGT GTCCATCGAT 
ATAGGTCCAA GTCCTGGCAA TGAGGTGAAT TTTGATTCAT CAGGTCGAGT TTTAGCTCAG 
GCAAGTGGCA ATGGTGTTAT CCATTTGCTA GATCTTAAAT CTGGGGAGAT TCACAAATTG 
ATGGGCCACG AAAACGAGGC ACACACGGTT GTGTTTTCTC ACGACGGGGA GATTCTCTTT 
TCTGGAGGCT CTGACGGCAC AGTTCGAACG TGGTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71891
Clone ID Related Accession (Same CDS sequence) XM_017004896.1 , XM_017004897.1
Accession Version XM_017004897.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1539bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X11
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGAATGA CCAGAACTCT TGATTGCTTT CAGTCTGAAT GGTATGAGTT AATACAGAAA 
GGAGTGACTG AACTTAGAAC TGTTGGGAAT GTTCCAGATG TCTACACCCA GATTATGCTT 
TTGGAAAATG AGAACAAAAA TTTAAAGAAA GATTTGAAGC ACTACAAACA AGCAGCTGAC 
AAAGCTAGAG AAGATTTGCT GAAAATTCAG AAAGAACGTG ATTTTCATCG AATGCATCAT 
AAGCGAATAG TCCAGGAAAA AAACAAATTA ATTAATGACC TCAAAGGGTT GAAGTTACAT 
TATGCATCTT ATGAACCGAC TATAAGGGTG TTACATGAGA AACACCACAC TTTACTGAAG 
GAGAAAATGC TGACCTCCTT GGAAAGAGAC AAAGTAGTTG GGCAGATTTC TGGACTTCAA 
GAAACATTGA AGAAACTGCA AAGAGGACAT AGTTACCATG GTCCTCAAAT TAAAGTTGAT 
CATAGTCGTG AAAAAGAAAA TGCACCAGAA GGTCCTACTC AGAAAGGTCT TCGTGAAGCC 
AGGGAACAAA ACAAATGTAA AACAAAGATG AAAGGCAATA CAAAGGATTC AGAATTTCCC 
ATAGATATGC AACCAAATCC AAACCTGAAT GTTTCTAAAG AAAGTCTTTC TCCAGCAAAA 
TTTGACTACA AGCTGAAAAA CATTTTTAGA CTCCATGAAC TTCCAGTGAG CTGTGTCTCC 
ATGCAACCCC ACAAAGACAT CCTAGTCTCC TGTGGCGAGG ACCGACTCTG GAAGGTGTTG 
GGCCTTCCAA AATGCAATGT GCTTCTCACG GGATTTGGCC ACACTGACTG GCTTTCAGAC 
TGCTGCTTCC ATCCCAGTGG CGACAAATTG GCTACTTCAA GTGGTGACAC TACAGTTAAA 
TTATGGGATC TATGTAAAGG CGATTGCATT TTGACCTTTG AAGGACACAG CCGCGCAGTG 
TGGTCCTGCA CATGGCACTC CTGCGGCAAT TTTGTGGCTT CCTCCTCACT GGATAAAACT 
AGCAAAATTT GGGATGTTAA TAGTGAAAGA TGCAGATGTA CTTTGTATGG ACATACAGAT 
TCTGTGAACA GCATTGAGTT TTTTCCTTTC TCCAATACTC TTCTCACAAG CTCTGCAGAC 
AAGACCCTGT CTATATGGGA TGCAAGAACA GGTATATGTG AGCAGTCACT TTATGGTCAC 
ATGCATTCTA TCAATGATGC CATTTTTGAT CCCAGGGGTC ACATGATAGC ATCCTGTGAT 
GCCTGTGGGG TTACAAAGCT GTGGGACTTT CGGAAGCTGT TACCAATTGT GTCCATCGAT 
ATAGGTCCAA GTCCTGGCAA TGAGGTGAAT TTTGATTCAT CAGGTCGAGT TTTAGCTCAG 
GCAAGTGGCA ATGGTGTTAT CCATTTGCTA GATCTTAAAT CTGGGGAGAT TCACAAATTG 
ATGGGCCACG AAAACGAGGC ACACACGGTT GTGTTTTCTC ACGACGGGGA GATTCTCTTT 
TCTGGAGGCT CTGACGGCAC AGTTCGAACG TGGTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860386.1
CDS3160..4698
Misc Feature(1)3280..3615
Misc Feature(2)<3823..4695
Misc Feature(3)3832..4695
Misc Feature(4)order(3853..3855,3907..3909,3919..3921,3937..3942,3976..3981,4033..4035,4045..4047,4063..4068,4102..4107,4156..4158,4171..4173,4189..4194,4231..4233,4282..4284,4297..4299,4315..4320,4354..4359,4411..4413,4423..4425,4441..4446,4480..4485,4534..4536,4549..4551,4567..4572,4603..4608,4660..4662,4672..4674,4690..4695)
Misc Feature(5)3865..3978
Misc Feature(6)3994..4104
Misc Feature(7)4117..4227
Misc Feature(8)4246..4350
Misc Feature(9)4369..4479
Misc Feature(10)4495..4602
Misc Feature(11)4621..4692
Translation

Target ORF information:

RefSeq Version XM_017004897.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X13, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004897.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGGGAATGA CCAGAACTCT TGATTGCTTT CAGTCTGAAT GGTATGAGTT AATACAGAAA 
GGAGTGACTG AACTTAGAAC TGTTGGGAAT GTTCCAGATG TCTACACCCA GATTATGCTT 
TTGGAAAATG AGAACAAAAA TTTAAAGAAA GATTTGAAGC ACTACAAACA AGCAGCTGAC 
AAAGCTAGAG AAGATTTGCT GAAAATTCAG AAAGAACGTG ATTTTCATCG AATGCATCAT 
AAGCGAATAG TCCAGGAAAA AAACAAATTA ATTAATGACC TCAAAGGGTT GAAGTTACAT 
TATGCATCTT ATGAACCGAC TATAAGGGTG TTACATGAGA AACACCACAC TTTACTGAAG 
GAGAAAATGC TGACCTCCTT GGAAAGAGAC AAAGTAGTTG GGCAGATTTC TGGACTTCAA 
GAAACATTGA AGAAACTGCA AAGAGGACAT AGTTACCATG GTCCTCAAAT TAAAGTTGAT 
CATAGTCGTG AAAAAGAAAA TGCACCAGAA GGTCCTACTC AGAAAGGTCT TCGTGAAGCC 
AGGGAACAAA ACAAATGTAA AACAAAGATG AAAGGCAATA CAAAGGATTC AGAATTTCCC 
ATAGATATGC AACCAAATCC AAACCTGAAT GTTTCTAAAG AAAGTCTTTC TCCAGCAAAA 
TTTGACTACA AGCTGAAAAA CATTTTTAGA CTCCATGAAC TTCCAGTGAG CTGTGTCTCC 
ATGCAACCCC ACAAAGACAT CCTAGTCTCC TGTGGCGAGG ACCGACTCTG GAAGGTGTTG 
GGCCTTCCAA AATGCAATGT GCTTCTCACG GGATTTGGCC ACACTGACTG GCTTTCAGAC 
TGCTGCTTCC ATCCCAGTGG CGACAAATTG GCTACTTCAA GTGGTGACAC TACAGTTAAA 
TTATGGGATC TATGTAAAGG CGATTGCATT TTGACCTTTG AAGGACACAG CCGCGCAGTG 
TGGTCCTGCA CATGGCACTC CTGCGGCAAT TTTGTGGCTT CCTCCTCACT GGATAAAACT 
AGCAAAATTT GGGATGTTAA TAGTGAAAGA TGCAGATGTA CTTTGTATGG ACATACAGAT 
TCTGTGAACA GCATTGAGTT TTTTCCTTTC TCCAATACTC TTCTCACAAG CTCTGCAGAC 
AAGACCCTGT CTATATGGGA TGCAAGAACA GGTATATGTG AGCAGTCACT TTATGGTCAC 
ATGCATTCTA TCAATGATGC CATTTTTGAT CCCAGGGGTC ACATGATAGC ATCCTGTGAT 
GCCTGTGGGG TTACAAAGCT GTGGGACTTT CGGAAGCTGT TACCAATTGT GTCCATCGAT 
ATAGGTCCAA GTCCTGGCAA TGAGGTGAAT TTTGATTCAT CAGGTCGAGT TTTAGCTCAG 
GCAAGTGGCA ATGGTGTTAT CCATTTGCTA GATCTTAAAT CTGGGGAGAT TCACAAATTG 
ATGGGCCACG AAAACGAGGC ACACACGGTT GTGTTTTCTC ACGACGGGGA GATTCTCTTT 
TCTGGAGGCT CTGACGGCAC AGTTCGAACG TGGTCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71893
Clone ID Related Accession (Same CDS sequence) XM_011511827.1
Accession Version XM_011511827.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X13
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGCCTCCTT CAACAGAAAT 
CAAGAGAAAG CTTATGAGCT CAGTGCGAGA ACAGCCCTCT GTCTAACTCC ATGCCTCCAT 
CAGCAGCAGT GGACAGGGAA CTCTCAAACT TGGAATAAGA CCGTACCTCT GCAGGACACT 
ATTGGCAGGA GACAAGAGAA CAACAGTGCA CACCAGCACA TCGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510129.1
CDS57..1184
Translation

Target ORF information:

RefSeq Version XM_011511827.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511827.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGCCTCCTT CAACAGAAAT 
CAAGAGAAAG CTTATGAGCT CAGTGCGAGA ACAGCCCTCT GTCTAACTCC ATGCCTCCAT 
CAGCAGCAGT GGACAGGGAA CTCTCAAACT TGGAATAAGA CCGTACCTCT GCAGGACACT 
ATTGGCAGGA GACAAGAGAA CAACAGTGCA CACCAGCACA TCGACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71894
Clone ID Related Accession (Same CDS sequence) XM_011511828.1
Accession Version XM_011511828.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1098bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X14
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG GAATCTGGAT 
GAATACCATA CTATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510130.1
CDS57..1154
Translation

Target ORF information:

RefSeq Version XM_011511828.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X16, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511828.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG GAATCTGGAT 
GAATACCATA CTATTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71895
Clone ID Related Accession (Same CDS sequence) XM_011511829.1
Accession Version XM_011511829.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X15
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGCTTCCTTT 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510131.1
CDS57..1139
Translation

Target ORF information:

RefSeq Version XM_011511829.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X17, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511829.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG TGCTTCCTTT 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71897
Clone ID Related Accession (Same CDS sequence) XM_011511832.1 , XM_011511831.1
Accession Version XM_011511832.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X17
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510134.1
CDS57..1127
Translation

Target ORF information:

RefSeq Version XM_011511832.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X19, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511832.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71897
Clone ID Related Accession (Same CDS sequence) XM_011511832.1 , XM_011511831.1
Accession Version XM_011511831.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1071bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X16
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510133.1
CDS57..1127
Translation

Target ORF information:

RefSeq Version XM_011511831.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511831.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGGATTCAGA ATTTCCCATA 
GATATGCAAC CAAATCCAAA CCTGAATGTT TCTAAAGAAA GTCTTTCTCC AGCAAAATTT 
GACTACAAGC TGAAAAACAT TTTTAGACTC CATGAACTTC CAGTGAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71898
Clone ID Related Accession (Same CDS sequence) XM_017004902.1 , XM_011511835.2 , XM_017004900.1
Accession Version XM_017004902.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X20
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTCCTCA AATTAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860391.1
CDS180..1151
Misc Feature(1)<276..1148
Misc Feature(2)285..1148
Misc Feature(3)order(306..308,360..362,372..374,390..395,429..434,486..488,498..500,516..521,555..560,609..611,624..626,642..647,684..686,735..737,750..752,768..773,807..812,864..866,876..878,894..899,933..938,987..989,1002..1004,1020..1025,1056..1061,1113..1115,1125..1127,1143..1148)
Misc Feature(4)318..431
Misc Feature(5)447..557
Misc Feature(6)570..680
Misc Feature(7)699..803
Misc Feature(8)822..932
Misc Feature(9)948..1055
Misc Feature(10)1074..1145
Translation

Target ORF information:

RefSeq Version XM_017004902.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X25, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004902.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTCCTCA AATTAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71898
Clone ID Related Accession (Same CDS sequence) XM_017004902.1 , XM_011511835.2 , XM_017004900.1
Accession Version XM_011511835.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X20
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTCCTCA AATTAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510137.1
CDS963..1934
Misc Feature(1)<1059..1931
Misc Feature(2)1068..1931
Misc Feature(3)order(1089..1091,1143..1145,1155..1157,1173..1178,1212..1217,1269..1271,1281..1283,1299..1304,1338..1343,1392..1394,1407..1409,1425..1430,1467..1469,1518..1520,1533..1535,1551..1556,1590..1595,1647..1649,1659..1661,1677..1682,1716..1721,1770..1772,1785..1787,1803..1808,1839..1844,1896..1898,1908..1910,1926..1931)
Misc Feature(4)1101..1214
Misc Feature(5)1230..1340
Misc Feature(6)1353..1463
Misc Feature(7)1482..1586
Misc Feature(8)1605..1715
Misc Feature(9)1731..1838
Misc Feature(10)1857..1928
Translation

Target ORF information:

RefSeq Version XM_011511835.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X24, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511835.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTCCTCA AATTAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71898
Clone ID Related Accession (Same CDS sequence) XM_017004902.1 , XM_011511835.2 , XM_017004900.1
Accession Version XM_017004900.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X20
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTCCTCA AATTAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860389.1
CDS862..1833
Misc Feature(1)<958..1830
Misc Feature(2)967..1830
Misc Feature(3)order(988..990,1042..1044,1054..1056,1072..1077,1111..1116,1168..1170,1180..1182,1198..1203,1237..1242,1291..1293,1306..1308,1324..1329,1366..1368,1417..1419,1432..1434,1450..1455,1489..1494,1546..1548,1558..1560,1576..1581,1615..1620,1669..1671,1684..1686,1702..1707,1738..1743,1795..1797,1807..1809,1825..1830)
Misc Feature(4)1000..1113
Misc Feature(5)1129..1239
Misc Feature(6)1252..1362
Misc Feature(7)1381..1485
Misc Feature(8)1504..1614
Misc Feature(9)1630..1737
Misc Feature(10)1756..1827
Translation

Target ORF information:

RefSeq Version XM_017004900.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X22, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004900.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGTCCTCA AATTAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu71899
Clone ID Related Accession (Same CDS sequence) XM_011511837.2
Accession Version XM_011511837.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 963bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X22
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGAAATCAAG AGAAAGCTTA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011510139.1
CDS57..1019
Translation

Target ORF information:

RefSeq Version XM_011511837.2
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X26, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011511837.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGATCAT 
AGTCGTGAAA AAGAAAATGC ACCAGAAGGT CCTACTCAGA AAGGTCTTCG TGAAGCCAGG 
GAACAAAACA AATGTAAAAC AAAGATGAAA GGCAATACAA AGAAATCAAG AGAAAGCTTA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89900
Clone ID Related Accession (Same CDS sequence) XM_017004898.1
Accession Version XM_017004898.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1173bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X12
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTGACCT CCTTGGAAAG AGACAAAGTA GTTGGGCAGA TTTCTGGACT TCAAGAAACA 
TTGAAGAAAC TGCAAAGAGG ACATAGTTAC CATGGTCCTC AAATTAAAGT TGATCATAGT 
CGTGAAAAAG AAAATGCACC AGAAGGTCCT ACTCAGAAAG GTCTTCGTGA AGCCAGGGAA 
CAAAACAAAT GTAAAACAAA GATGAAAGGC AATACAAAGG ATTCAGAATT TCCCATAGAT 
ATGCAACCAA ATCCAAACCT GAATGTTTCT AAAGAAAGTC TTTCTCCAGC AAAATTTGAC 
TACAAGCTGA AAAACATTTT TAGACTCCAT GAACTTCCAG TGAGCTGTGT CTCCATGCAA 
CCCCACAAAG ACATCCTAGT CTCCTGTGGC GAGGACCGAC TCTGGAAGGT GTTGGGCCTT 
CCAAAATGCA ATGTGCTTCT CACGGGATTT GGCCACACTG ACTGGCTTTC AGACTGCTGC 
TTCCATCCCA GTGGCGACAA ATTGGCTACT TCAAGTGGTG ACACTACAGT TAAATTATGG 
GATCTATGTA AAGGCGATTG CATTTTGACC TTTGAAGGAC ACAGCCGCGC AGTGTGGTCC 
TGCACATGGC ACTCCTGCGG CAATTTTGTG GCTTCCTCCT CACTGGATAA AACTAGCAAA 
ATTTGGGATG TTAATAGTGA AAGATGCAGA TGTACTTTGT ATGGACATAC AGATTCTGTG 
AACAGCATTG AGTTTTTTCC TTTCTCCAAT ACTCTTCTCA CAAGCTCTGC AGACAAGACC 
CTGTCTATAT GGGATGCAAG AACAGGTATA TGTGAGCAGT CACTTTATGG TCACATGCAT 
TCTATCAATG ATGCCATTTT TGATCCCAGG GGTCACATGA TAGCATCCTG TGATGCCTGT 
GGGGTTACAA AGCTGTGGGA CTTTCGGAAG CTGTTACCAA TTGTGTCCAT CGATATAGGT 
CCAAGTCCTG GCAATGAGGT GAATTTTGAT TCATCAGGTC GAGTTTTAGC TCAGGCAAGT 
GGCAATGGTG TTATCCATTT GCTAGATCTT AAATCTGGGG AGATTCACAA ATTGATGGGC 
CACGAAAACG AGGCACACAC GGTTGTGTTT TCTCACGACG GGGAGATTCT CTTTTCTGGA 
GGCTCTGACG GCACAGTTCG AACGTGGTCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860387.1
CDS258..1430
Translation

Target ORF information:

RefSeq Version XM_017004898.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004898.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCTGACCT CCTTGGAAAG AGACAAAGTA GTTGGGCAGA TTTCTGGACT TCAAGAAACA 
TTGAAGAAAC TGCAAAGAGG ACATAGTTAC CATGGTCCTC AAATTAAAGT TGATCATAGT 
CGTGAAAAAG AAAATGCACC AGAAGGTCCT ACTCAGAAAG GTCTTCGTGA AGCCAGGGAA 
CAAAACAAAT GTAAAACAAA GATGAAAGGC AATACAAAGG ATTCAGAATT TCCCATAGAT 
ATGCAACCAA ATCCAAACCT GAATGTTTCT AAAGAAAGTC TTTCTCCAGC AAAATTTGAC 
TACAAGCTGA AAAACATTTT TAGACTCCAT GAACTTCCAG TGAGCTGTGT CTCCATGCAA 
CCCCACAAAG ACATCCTAGT CTCCTGTGGC GAGGACCGAC TCTGGAAGGT GTTGGGCCTT 
CCAAAATGCA ATGTGCTTCT CACGGGATTT GGCCACACTG ACTGGCTTTC AGACTGCTGC 
TTCCATCCCA GTGGCGACAA ATTGGCTACT TCAAGTGGTG ACACTACAGT TAAATTATGG 
GATCTATGTA AAGGCGATTG CATTTTGACC TTTGAAGGAC ACAGCCGCGC AGTGTGGTCC 
TGCACATGGC ACTCCTGCGG CAATTTTGTG GCTTCCTCCT CACTGGATAA AACTAGCAAA 
ATTTGGGATG TTAATAGTGA AAGATGCAGA TGTACTTTGT ATGGACATAC AGATTCTGTG 
AACAGCATTG AGTTTTTTCC TTTCTCCAAT ACTCTTCTCA CAAGCTCTGC AGACAAGACC 
CTGTCTATAT GGGATGCAAG AACAGGTATA TGTGAGCAGT CACTTTATGG TCACATGCAT 
TCTATCAATG ATGCCATTTT TGATCCCAGG GGTCACATGA TAGCATCCTG TGATGCCTGT 
GGGGTTACAA AGCTGTGGGA CTTTCGGAAG CTGTTACCAA TTGTGTCCAT CGATATAGGT 
CCAAGTCCTG GCAATGAGGT GAATTTTGAT TCATCAGGTC GAGTTTTAGC TCAGGCAAGT 
GGCAATGGTG TTATCCATTT GCTAGATCTT AAATCTGGGG AGATTCACAA ATTGATGGGC 
CACGAAAACG AGGCACACAC GGTTGTGTTT TCTCACGACG GGGAGATTCT CTTTTCTGGA 
GGCTCTGACG GCACAGTTCG AACGTGGTCT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89901
Clone ID Related Accession (Same CDS sequence) XM_017004899.1
Accession Version XM_017004899.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X18
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGCTTCA 
AAGAGAATAA AATACCTAAG AATCCAACTT ACAAAGGATG TGAAGGACGT CTTCAAGGAG 
AACTACAAAC CACTGCTCAA TGAAGTAAAA GAGGACACAA ACAAATGGAA GAACATTCCA 
TGCTCATGGA TAGGAAGAAT CAATATCATG ATAATGGCCA TACTGCGCGA GAAAATCTTG 
AAAGCAATAA TACAAATACA GGCTGATTTC TCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860388.1
CDS57..1112
Translation

Target ORF information:

RefSeq Version XM_017004899.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X20, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004899.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGCTC AGCGAGGGAT GCCCAGCTCC GCCGTGAGGG TCCTGGAAGA GGCGTTGGGC 
ATGGGTTTGA CGGCAGCCGG GGACGCGAGG GACACGGCGG ACGCGGTGGC GGCTGAGGGC 
GCCTACTACC TGGAACAGGT CACCATAACT GAAGCATCTG AAGATGACTA TGAATATGAA 
GAGATACCAG ATGACAATTT TAGCATCCCA GAAGGTGAAG AAGATCTGGC AAAAGCAATT 
CAGATGGCCC AAGAACAGGC TACAGATACT GAAATTTTGG AACGGAAAAC AGTTCTTCCT 
TCAAAGCATG CAGTACCTGA AGTAATAGAA GACTTTCTCT GCAATTTCTT GATCAAAATG 
GGAATGACCA GAACTCTTGA TTGCTTTCAG TCTGAATGGT ATGAGTTAAT ACAGAAAGGA 
GTGACTGAAC TTAGAACTGT TGGGAATGTT CCAGATGTCT ACACCCAGAT TATGCTTTTG 
GAAAATGAGA ACAAAAATTT AAAGAAAGAT TTGAAGCACT ACAAACAAGC AGCTGACAAA 
GCTAGAGAAG ATTTGCTGAA AATTCAGAAA GAACGTGATT TTCATCGAAT GCATCATAAG 
CGAATAGTCC AGGAAAAAAA CAAATTAATT AATGACCTCA AAGGGTTGAA GTTACATTAT 
GCATCTTATG AACCGACTAT AAGGGTGTTA CATGAGAAAC ACCACACTTT ACTGAAGGAG 
AAAATGCTGA CCTCCTTGGA AAGAGACAAA GTAGTTGGGC AGATTTCTGG ACTTCAAGAA 
ACATTGAAGA AACTGCAAAG AGGACATAGT TACCATGGTC CTCAAATTAA AGTTGCTTCA 
AAGAGAATAA AATACCTAAG AATCCAACTT ACAAAGGATG TGAAGGACGT CTTCAAGGAG 
AACTACAAAC CACTGCTCAA TGAAGTAAAA GAGGACACAA ACAAATGGAA GAACATTCCA 
TGCTCATGGA TAGGAAGAAT CAATATCATG ATAATGGCCA TACTGCGCGA GAAAATCTTG 
AAAGCAATAA TACAAATACA GGCTGATTTC TCATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu89902
Clone ID Related Accession (Same CDS sequence) XM_017004901.1
Accession Version XM_017004901.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 972bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product sperm-associated antigen 16 protein isoform X21
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAAAGGCA ATACAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016860390.1
CDS844..1815
Translation

Target ORF information:

RefSeq Version XM_017004901.1
Organism Homo sapiens(human)
Definition Homo sapiens sperm associated antigen 16 (SPAG16), transcript variant X23, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017004901.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGAAAGGCA ATACAAAGGA TTCAGAATTT CCCATAGATA TGCAACCAAA TCCAAACCTG 
AATGTTTCTA AAGAAAGTCT TTCTCCAGCA AAATTTGACT ACAAGCTGAA AAACATTTTT 
AGACTCCATG AACTTCCAGT GAGCTGTGTC TCCATGCAAC CCCACAAAGA CATCCTAGTC 
TCCTGTGGCG AGGACCGACT CTGGAAGGTG TTGGGCCTTC CAAAATGCAA TGTGCTTCTC 
ACGGGATTTG GCCACACTGA CTGGCTTTCA GACTGCTGCT TCCATCCCAG TGGCGACAAA 
TTGGCTACTT CAAGTGGTGA CACTACAGTT AAATTATGGG ATCTATGTAA AGGCGATTGC 
ATTTTGACCT TTGAAGGACA CAGCCGCGCA GTGTGGTCCT GCACATGGCA CTCCTGCGGC 
AATTTTGTGG CTTCCTCCTC ACTGGATAAA ACTAGCAAAA TTTGGGATGT TAATAGTGAA 
AGATGCAGAT GTACTTTGTA TGGACATACA GATTCTGTGA ACAGCATTGA GTTTTTTCCT 
TTCTCCAATA CTCTTCTCAC AAGCTCTGCA GACAAGACCC TGTCTATATG GGATGCAAGA 
ACAGGTATAT GTGAGCAGTC ACTTTATGGT CACATGCATT CTATCAATGA TGCCATTTTT 
GATCCCAGGG GTCACATGAT AGCATCCTGT GATGCCTGTG GGGTTACAAA GCTGTGGGAC 
TTTCGGAAGC TGTTACCAAT TGTGTCCATC GATATAGGTC CAAGTCCTGG CAATGAGGTG 
AATTTTGATT CATCAGGTCG AGTTTTAGCT CAGGCAAGTG GCAATGGTGT TATCCATTTG 
CTAGATCTTA AATCTGGGGA GATTCACAAA TTGATGGGCC ACGAAAACGA GGCACACACG 
GTTGTGTTTT CTCACGACGG GGAGATTCTC TTTTCTGGAG GCTCTGACGG CACAGTTCGA 
ACGTGGTCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.
BMC molecular biology18(1)2(2017 01)
Zhang L,Liu Y,Li W,Zhang Q,Li Y,Liu J,Min J,Shuang C,Song S,Zhang Z


book

Identification of a genetic variant for joint damage progression in autoantibody-positive rheumatoid arthritis.
Annals of the rheumatic diseases73(11)2038-46(2014 Nov)
Knevel R,Klein K,Somers K,Ospelt C,Houwing-Duistermaat JJ,van Nies JA,de Rooy DP,de Bock L,Kurreeman FA,Schonkeren J,Stoeken-Rijsbergen G,Helmer Q,van der Linden MP,Kern M,Manjarrez-Orduno N,Rodriguez-Rodriquez L,Stinissen P,Huizinga TW,Toes RE,Gay S,Gregersen PK,Somers V,van der Helm-van Mil AH


book

Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis.
Journal of immunology (Baltimore, Md. : 1950)193(5)2147-56(2014 Sep)
de Bock L,Somers K,Fraussen J,Hendriks JJ,van Horssen J,Rouwette M,Hellings N,Villar LM,Alvarez-Cerme?o JC,Espi?o M,Hupperts R,Jongen P,Damoiseaux J,Verbeek MM,De Deyn PP,D'hooghe M,Van Wijmeersch B,Stinissen P,Somers V


book

Variants in the 1q21 risk region are associated with a visual endophenotype of autism and schizophrenia.
Genes, brain, and behavior13(2)144-51(2014 Feb)
Goodbourn PT,Bosten JM,Bargary G,Hogg RE,Lawrance-Owen AJ,Mollon JD


book

Genome-wide contribution of genotype by environment interaction to variation of diabetes-related traits.
PloS one8(10)e77442(2013)
Zheng JS,Arnett DK,Lee YC,Shen J,Parnell LD,Smith CE,Richardson K,Li D,Borecki IB,Ordov?s JM,Lai CQ