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LRRK1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol LRRK1
Entrez Gene ID 79705
Full Name leucine rich repeat kinase 1
Synonyms RIPK6,Roco1
General protein information
Preferred Names

leucine rich repeat kinase 1

Names

leucine-rich repeat serine/threonine-protein kinase 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

15

15q26.3

Summary This gene encodes a multi-domain protein that is a leucine-rich repeat kinase and a GDP/GTP binding protein. The encoded protein is thought to play a role in the regulation of bone mass. Mice lacking a similar gene showed severe osteopetrosis, increased bone mineralization and decreased bone resorption. [provided by RefSeq, Jan 2017].
Disorder MIM:

610986


mRNA and Protein(s)

mRNA Protein Name
XM_017022570.1 XP_016878059.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X5
XM_011522012.2 XP_011520314.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_011522016.2 XP_011520318.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X4
XM_005254979.3 XP_005255036.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X6
XM_011522018.2 XP_011520320.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X6
NM_024652.5 NP_078928.3 leucine-rich repeat serine/threonine-protein kinase 1
XM_011522015.2 XP_011520317.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X3
XM_011522019.2 XP_011520321.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X7
XM_011522013.2 XP_011520315.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_011522014.2 XP_011520316.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
XM_011522017.2 XP_011520319.1 leucine-rich repeat serine/threonine-protein kinase 1 isoform X6



Danio rerio (zebrafish) lrrk1 XP_002667476.3
Bos taurus (cattle) LRRK1 NP_001192703.1
Xenopus tropicalis (tropical clawed frog) lrrk1 XP_002939309.2
Homo sapiens (human) LRRK1 NP_078928.3
Pan troglodytes (chimpanzee) LRRK1 XP_510623.3
Rattus norvegicus (Norway rat) Lrrk1 NP_001178553.1
Canis lupus familiaris (dog) LRRK1 XP_005618338.1
Macaca mulatta (rhesus monkey) LRRK1 XP_001084079.1
Mus musculus (house mouse) Lrrk1 NP_666303.3
Gallus gallus (chicken) LRRK1 XP_003641870.2


Related articles in PubMed

Identification of biallelic LRRK1 mutations in osteosclerotic metaphyseal dysplasia and evidence for locus heterogeneity.
Iida A, Xing W, Docx MK, Nakashima T, Wang Z, Kimizuka M, Van Hul W, Rating D, Spranger J, Ohashi H, Miyake N, Matsumoto N, Mohan S, Nishimura G, Mortier G, Ikegawa S
Journal of medical genetics53(8)568-74(2016 Aug)

PLK1-dependent activation of LRRK1 regulates spindle orientation by phosphorylating CDK5RAP2.
Hanafusa H, Kedashiro S, Tezuka M, Funatsu M, Usami S, Toyoshima F, Matsumoto K
Nature cell biology17(8)1024-35(2015 Aug)

LRRK1-phosphorylated CLIP-170 regulates EGFR trafficking by recruiting p150Glued to microtubule plus ends.
Kedashiro S, Pastuhov SI, Nishioka T, Watanabe T, Kaibuchi K, Matsumoto K, Hanafusa H
Journal of cell science128(2)385-96(2015 Jan)

Differential protein-protein interactions of LRRK1 and LRRK2 indicate roles in distinct cellular signaling pathways.
Reyniers L, Del Giudice MG, Civiero L, Belluzzi E, Lobbestael E, Beilina A, Arrigoni G, Derua R, Waelkens E, Li Y, Crosio C, Iaccarino C, Cookson MR, Baekelandt V, Greggio E, Taymans JM
Journal of neurochemistry131(2)239-50(2014 Oct)

Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
Beilina A, Rudenko IN, Kaganovich A, Civiero L, Chau H, Kalia SK, Kalia LV, Lobbestael E, Chia R, Ndukwe K, Ding J, Nalls MA, , , Olszewski M, Hauser DN, Kumaran R, Lozano AM, Baekelandt V, Greene LE, Taymans JM, Greggio E, Cookson MR
Proceedings of the National Academy of Sciences of the United States of America111(7)2626-31(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel LRRK1 mutation has been identified in two siblings in an Indian family with osteosclerotic metaphyseal dysplasia.
Title: Identification of a novel LRRK1 mutation in a family with osteosclerotic metaphyseal dysplasia.

LRRK1 regulates mitotic spindle orientation downstream of PLK1 through CDK5RAP2-dependent centrosome maturation.
Title: PLK1-dependent activation of LRRK1 regulates spindle orientation by phosphorylating CDK5RAP2.

We find that LRRK1-mediated phosphorylation of CLIP-170 causes the accumulation of p150(Glued) (also known as DCTN1) a subunit of dynactin, at microtubule plus ends, thereby facilitating the migration of EGFR-containing endosomes.
Title: LRRK1-phosphorylated CLIP-170 regulates EGFR trafficking by recruiting p150Glued to microtubule plus ends.

LRRK1 carries out distinct functions from LRRK2 by interacting with different cellular proteins.
Title: Differential protein-protein interactions of LRRK1 and LRRK2 indicate roles in distinct cellular signaling pathways.

LRRK1 mutations may have a role in Parkinson's disease
Title: Rare variants in LRRK1 and Parkinson's disease.

The following LRRK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu48565 XM_011522017.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu48565 XM_011522018.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu48565 XM_005254979.3
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu72035 XM_011522019.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu85662 XM_017022570.1
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 24-26 $811.30
$1159.00
OHu72032 XM_011522014.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu72033 XM_011522015.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu72034 XM_011522016.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $951.30
$1359.00
OHu72031 NM_024652.5
Latest version!
Homo sapiens leucine rich repeat kinase 1 (LRRK1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu72031 XM_011522012.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00
OHu72031 XM_011522013.2
Latest version!
Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 30 $923.30
$1319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu48565
Clone ID Related Accession (Same CDS sequence) XM_005254979.3 , XM_011522018.2 , XM_011522017.2
Accession Version XM_005254979.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGCCACTG TCAACCAGTG CTTCTTCACG GACAAGGCCC TGTACGTGGT GGTCTGGAAC 
CTGGCGCTGG GGGAGGAGGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATCGAGGCC
AAGGCCCCAA ACGCCGTGGT GCTGGTGGTC GGGACGCACC TGGATTTAAT TGAAGCCAAG
TTCCGTGTGG AAAGGATTGC AACGCTGCGT GCCTATGTGC TGGCACTCTG CCGCTCCCCC
TCCGGCTCCA GGGCCACAGG CTTCCCAGAC ATCACCTTCA AACACTTACA TGAGATTTCC
TGCAAGAGCC TGGAAGGTCA GGAAGGGCTG CGACAGCTGA TTTTCCACGT CACGTGCAGC
ATGAAGGACG TGGGCAGCAC CATCGGCTGC CAGCGACTGG CAGGGCGGCT GATCCCCAGG
AGCTACCTGA GCCTGCAGGA GGCCGTGCTG GCAGAGCAGC AGCGCCGCAG CCGGGACGAC
GACGTGCAGT ACCTGACGGA CAGGCAGCTG GAGCAGCTGG TGGAGCAGAC GCCCGACAAC
GACATCAAGG ACTACGAGGA CCTGCAGTCA GCCATCAGCT TCCTCATAGA AACCGGCACC
CTGCTCCATT TCCCGGACAC CAGCCACGGC CTGAGGAACC TCTACTTCCT CGACCCTATT
TGGCTCTCCG AATGTCTGCA GAGGATCTTT AATATTAAGG GCTCTCGGTC AGTGGCCAAG
AATGGGGTGA TCAGAGCAGA AGACCTCAGG ATGCTGCTGG TGGGGACTGG CTTCACGCAG
CAGACGGAAG AGCAGTACTT CCAGTTCCTG GCCAAGTTTG AGATCGCCCT GCCCGTCGCC
AATGACAGCT ACCTCCTGCC CCATCTCCTT CCATCTAAAC CTGGCCTGGA CACCCACGGT
ATGCGGCACC CCACAGCCAA CACCATTCAG AGGGTATTTA AGATGAGCTT CGTTCCCGTT
GGCTTCTGGC AAAGGTTTAT AGCACGGATG CTGATCAGCC TGGCGGAGAT GGACCTGCAG
CTTTTTGAAA ACAAGAAGAA TACTAAAAGC AGGAACAGGA AAGTCACCAT TTACAGTTTT
ACAGGAAACC AGAGAAATCG CTGTAGCACA TTCAGAGTGA AAAGAAATCA GACCATCTAT
TGGCAGGAAG GGCTCCTGGT CACTTTTGAT GGGGGCTACC TCAGTGTGGA ATCTTCCGAC
GTGAACTGGA AAAAGAAGAA AAGCGGAGGA ATGAAAATTG TTTGCCAATC AGAAGTGAGG
GACTTCTCAG CCATGGCTTT CATCACGGAC CACGTCAATT CCTTGATTGA TCAGTGGTTT
CCCGCCCTGA CAGCCACAGA GAGCGACGGG ACGCCACTCA TGGAGCAGTA CGTGCCCTGC
CCGGTCTGCG AGACAGCCTG GGCCCAGCAC ACGGACCCCA GTGAGAAATC AGAGGATGTG
CAGTACTTCG ACATGGAAGA CTGTGTCCTG ACGGCCATCG AGCGGGACTT CATCTCCTGC
CCCAGACACC CGGACCTCCC CGTGCCGCTG CAGGAGCTGG TCCCTGAACT GTTCATGACC
GACTTCCCGG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACAGCGA GGACGAGGGC
AGCGTCCTGG GCCAGGGCGG CAGTGGCACC GTCATCTACC GGGCCCGGTA CCAGGGCCAG
CCTGTGGCCG TCAAGCGCTT CCACATCAAA AAATTCAAGA ACTTTGCTAA CGTACCGGCA
GACACCATGC TGAGGCACCT GCGGGCCACC GATGCCATGA AGAACTTCTC CGAGTTCCGG
CAGGAGGCCA GCATGCTGCA CGCGCTGCAG CACCCCTGCA TCGTGGCGCT CATCGGCATC
AGCATCCACC CGCTCTGCTT CGCCCTGGAG CTCGCGCCGC TCAGCAGCCT CAACACCGTG
CTGTCCGAGA ACGCCAGAGA TTCTTCCTTT ATACCCCTGG GACACATGCT CACCCAAAAA
ATAGCCTACC AGATCGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT
GACCTGAAGT CGGACAACAT TCTGGTGTGG TCCCTTGACG TCAAGGAGCA CATCAACATC
AAGCTATCTG ACTACGGGAT TTCGAGGCAG TCATTCCATG AGGGCGCCCT AGGCGTGGAG
GGCACTCCTG GCTACCAGGC CCCAGAGATC AGGCCTCGCA TTGTATATGA TGAGAAGGTA
GATATGTTCT CCTATGGAAT GGTGCTCTAC GAGTTGCTGT CAGGACAGCG CCCTGCACTG
GGCCACCACC AGCTCCAGAT TGCCAAGAAG CTGTCCAAGG GCATCCGCCC GGTTCTGGGG
CAGCCGGAGG AAGTGCAGTT CCGGCGACTG CAGGCGCTCA TGATGGAGTG CTGGGACACT
AAGCCAGAGA AGCGACCGCT GGCCCTGTCG GTGGTGAGCC AGATGAAGGA CCCGACTTTT
GCCACCTTCA TGTATGAACT GTGCTGTGGG AAGCAGACAG CCTTCTTCTC ATCCCAGGGC
CAGGAGTACA CCGTGGTGTT TTGGGATGGA AAAGAGGAGT CCAGGAACTA CACGGTGGTG
AACACAGAGA AGGGCCTCAT GGAGGTGCAG AGGATGTGCT GCCCTGGGAT GAAGGTGAGC
TGCCAGCTCC AGGTCCAGAG ATCCCTGTGG ACAGCCACCG AGGACCAGAA AATCTACATC
TACACCCTCA AGGGCATGTG CCCCTTAAAC ACACCCCAAC AGGCCTTGGA TACTCCAGCT
GTCGTCACCT GCTTCTTGGC CGTGCCTGTT ATTAAAAAGA ATTCCTACCT GGTCTTAGCG
GGCCTCGCCG ATGGGCTTGT GGCTGTGTTT CCCGTGGTGC GGGGCACCCC AAAGGACAGC
TGCTCCTACC TGTGCTCACA CACAGCCAAC AGGTCCAAGT TCAGCATCGC GGATGAAGAC
GCACGGCAGA ACCCCTACCC AGTGAAGGCC ATGGAGGTGG TCAACAGCGG CTCTGAGGTC
TGGTACAGCA ATGGGCCGGG CCTCCTTGTC ATCGACTGTG CCTCCCTGGA GATCTGCAGG
CGGCTGGAGC CCTACATGGC CCCCTCCATG GTTACGTCAG TCGTGTGCAG CTCTGAGGGC
AGAGGGGAGG AGGTCGTCTG GTGCCTGGAT GACAAGGCCA ACTCCTTGGT GATGTACCAC
TCCACCACCT ACCAGCTGTG TGCCCGGTAC TTCTGCGGGG TCCCCAGCCC CCTCAGGGAC
ATGTTTCCCG TGCGGCCCTT GGACACGGAA CCCCCGGCAG CCAGCCACAC GGCCAACCCA
AAGGTGCCTG AGGGGGACTC CATCGCGGAC GTGAGCATCA TGTACAGTGA GGAGCTGGGC
ACGCAGATCC TGATCCACCA GGAATCACTC ACTGACTACT GCTCCATGTC CTCCTACTCC
TCATCCCCAC CCCGCCAGGC TGCCAGGTCC CCCTCAAGCC TCCCCAGCTC CCCAGCAAGT
TCTTCCAGTG TGCCTTTCTC CACCGACTGC GAGGACTCAG ACATGCTACA TACGCCCGGT
GCTGCCTCCG ACAGGTCTGA GCATGACCTG ACCCCCATGG ACGGGGAGAC CTTCAGCCAG
CACCTGCAGG CCGTGAAGAT CCTCGCCGTC AGAGACCTCA TTTGGGTCCC CAGGCGCGGT
GGAGATGTTA TCGTCATTGG CCTGGAGAAG GATTCTGGCG CCCAGCGGGG CCGAGTCATT
GCCGTCTTAA AAGCCCGAGA GCTGACTCCG CATGGGGTGC TGGTGGATGC TGCCGTGGTG
GCAAAGGACA CTGTTGTGTG CACCTTTGAA AATGAAAACA CAGAGTGGTG CCTGGCCGTC
TGGAGGGGCT GGGGCGCCAG GGAGTTCGAC ATTTTCTACC AGTCCTACGA GGAGCTGGGC
CGGCTGGAGG CTTGCACTCG CAAGAGAAGG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005255036.1
CDS133..4065
Misc Feature(1)<148..480
Misc Feature(2)286..297
Misc Feature(3)430..438
Misc Feature(4)547..1125
Misc Feature(5)1750..2550
Misc Feature(6)1759..2586
Misc Feature(7)order(1759..1773,1783..1785,1819..1821,1825..1827,1975..1977,2017..2028,2038..2040,2044..2046,2173..2175,2179..2181,2185..2190,2194..2196,2242..2244,2251..2253,2293..2304)
Misc Feature(8)order(1759..1773,1783..1785,1819..1821,1825..1827,1975..1977,2017..2028,2038..2040,2173..2175,2179..2181,2185..2190,2194..2196,2242..2244)
Misc Feature(9)order(1771..1773,2038..2040,2044..2046,2173..2175,2179..2181,2185..2187,2251..2253,2293..2304)
Misc Feature(10)2239..2304
Translation

Target ORF information:

RefSeq Version XM_005254979.3
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005254979.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGCCACTG TCAACCAGTG CTTCTTCACG GACAAGGCCC TGTACGTGGT GGTCTGGAAC 
CTGGCGCTGG GGGAGGAGGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATCGAGGCC
AAGGCCCCAA ACGCCGTGGT GCTGGTGGTC GGGACGCACC TGGATTTAAT TGAAGCCAAG
TTCCGTGTGG AAAGGATTGC AACGCTGCGT GCCTATGTGC TGGCACTCTG CCGCTCCCCC
TCCGGCTCCA GGGCCACAGG CTTCCCAGAC ATCACCTTCA AACACTTACA TGAGATTTCC
TGCAAGAGCC TGGAAGGTCA GGAAGGGCTG CGACAGCTGA TTTTCCACGT CACGTGCAGC
ATGAAGGACG TGGGCAGCAC CATCGGCTGC CAGCGACTGG CAGGGCGGCT GATCCCCAGG
AGCTACCTGA GCCTGCAGGA GGCCGTGCTG GCAGAGCAGC AGCGCCGCAG CCGGGACGAC
GACGTGCAGT ACCTGACGGA CAGGCAGCTG GAGCAGCTGG TGGAGCAGAC GCCCGACAAC
GACATCAAGG ACTACGAGGA CCTGCAGTCA GCCATCAGCT TCCTCATAGA AACCGGCACC
CTGCTCCATT TCCCGGACAC CAGCCACGGC CTGAGGAACC TCTACTTCCT CGACCCTATT
TGGCTCTCCG AATGTCTGCA GAGGATCTTT AATATTAAGG GCTCTCGGTC AGTGGCCAAG
AATGGGGTGA TCAGAGCAGA AGACCTCAGG ATGCTGCTGG TGGGGACTGG CTTCACGCAG
CAGACGGAAG AGCAGTACTT CCAGTTCCTG GCCAAGTTTG AGATCGCCCT GCCCGTCGCC
AATGACAGCT ACCTCCTGCC CCATCTCCTT CCATCTAAAC CTGGCCTGGA CACCCACGGT
ATGCGGCACC CCACAGCCAA CACCATTCAG AGGGTATTTA AGATGAGCTT CGTTCCCGTT
GGCTTCTGGC AAAGGTTTAT AGCACGGATG CTGATCAGCC TGGCGGAGAT GGACCTGCAG
CTTTTTGAAA ACAAGAAGAA TACTAAAAGC AGGAACAGGA AAGTCACCAT TTACAGTTTT
ACAGGAAACC AGAGAAATCG CTGTAGCACA TTCAGAGTGA AAAGAAATCA GACCATCTAT
TGGCAGGAAG GGCTCCTGGT CACTTTTGAT GGGGGCTACC TCAGTGTGGA ATCTTCCGAC
GTGAACTGGA AAAAGAAGAA AAGCGGAGGA ATGAAAATTG TTTGCCAATC AGAAGTGAGG
GACTTCTCAG CCATGGCTTT CATCACGGAC CACGTCAATT CCTTGATTGA TCAGTGGTTT
CCCGCCCTGA CAGCCACAGA GAGCGACGGG ACGCCACTCA TGGAGCAGTA CGTGCCCTGC
CCGGTCTGCG AGACAGCCTG GGCCCAGCAC ACGGACCCCA GTGAGAAATC AGAGGATGTG
CAGTACTTCG ACATGGAAGA CTGTGTCCTG ACGGCCATCG AGCGGGACTT CATCTCCTGC
CCCAGACACC CGGACCTCCC CGTGCCGCTG CAGGAGCTGG TCCCTGAACT GTTCATGACC
GACTTCCCGG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACAGCGA GGACGAGGGC
AGCGTCCTGG GCCAGGGCGG CAGTGGCACC GTCATCTACC GGGCCCGGTA CCAGGGCCAG
CCTGTGGCCG TCAAGCGCTT CCACATCAAA AAATTCAAGA ACTTTGCTAA CGTACCGGCA
GACACCATGC TGAGGCACCT GCGGGCCACC GATGCCATGA AGAACTTCTC CGAGTTCCGG
CAGGAGGCCA GCATGCTGCA CGCGCTGCAG CACCCCTGCA TCGTGGCGCT CATCGGCATC
AGCATCCACC CGCTCTGCTT CGCCCTGGAG CTCGCGCCGC TCAGCAGCCT CAACACCGTG
CTGTCCGAGA ACGCCAGAGA TTCTTCCTTT ATACCCCTGG GACACATGCT CACCCAAAAA
ATAGCCTACC AGATCGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT
GACCTGAAGT CGGACAACAT TCTGGTGTGG TCCCTTGACG TCAAGGAGCA CATCAACATC
AAGCTATCTG ACTACGGGAT TTCGAGGCAG TCATTCCATG AGGGCGCCCT AGGCGTGGAG
GGCACTCCTG GCTACCAGGC CCCAGAGATC AGGCCTCGCA TTGTATATGA TGAGAAGGTA
GATATGTTCT CCTATGGAAT GGTGCTCTAC GAGTTGCTGT CAGGACAGCG CCCTGCACTG
GGCCACCACC AGCTCCAGAT TGCCAAGAAG CTGTCCAAGG GCATCCGCCC GGTTCTGGGG
CAGCCGGAGG AAGTGCAGTT CCGGCGACTG CAGGCGCTCA TGATGGAGTG CTGGGACACT
AAGCCAGAGA AGCGACCGCT GGCCCTGTCG GTGGTGAGCC AGATGAAGGA CCCGACTTTT
GCCACCTTCA TGTATGAACT GTGCTGTGGG AAGCAGACAG CCTTCTTCTC ATCCCAGGGC
CAGGAGTACA CCGTGGTGTT TTGGGATGGA AAAGAGGAGT CCAGGAACTA CACGGTGGTG
AACACAGAGA AGGGCCTCAT GGAGGTGCAG AGGATGTGCT GCCCTGGGAT GAAGGTGAGC
TGCCAGCTCC AGGTCCAGAG ATCCCTGTGG ACAGCCACCG AGGACCAGAA AATCTACATC
TACACCCTCA AGGGCATGTG CCCCTTAAAC ACACCCCAAC AGGCCTTGGA TACTCCAGCT
GTCGTCACCT GCTTCTTGGC CGTGCCTGTT ATTAAAAAGA ATTCCTACCT GGTCTTAGCG
GGCCTCGCCG ATGGGCTTGT GGCTGTGTTT CCCGTGGTGC GGGGCACCCC AAAGGACAGC
TGCTCCTACC TGTGCTCACA CACAGCCAAC AGGTCCAAGT TCAGCATCGC GGATGAAGAC
GCACGGCAGA ACCCCTACCC AGTGAAGGCC ATGGAGGTGG TCAACAGCGG CTCTGAGGTC
TGGTACAGCA ATGGGCCGGG CCTCCTTGTC ATCGACTGTG CCTCCCTGGA GATCTGCAGG
CGGCTGGAGC CCTACATGGC CCCCTCCATG GTTACGTCAG TCGTGTGCAG CTCTGAGGGC
AGAGGGGAGG AGGTCGTCTG GTGCCTGGAT GACAAGGCCA ACTCCTTGGT GATGTACCAC
TCCACCACCT ACCAGCTGTG TGCCCGGTAC TTCTGCGGGG TCCCCAGCCC CCTCAGGGAC
ATGTTTCCCG TGCGGCCCTT GGACACGGAA CCCCCGGCAG CCAGCCACAC GGCCAACCCA
AAGGTGCCTG AGGGGGACTC CATCGCGGAC GTGAGCATCA TGTACAGTGA GGAGCTGGGC
ACGCAGATCC TGATCCACCA GGAATCACTC ACTGACTACT GCTCCATGTC CTCCTACTCC
TCATCCCCAC CCCGCCAGGC TGCCAGGTCC CCCTCAAGCC TCCCCAGCTC CCCAGCAAGT
TCTTCCAGTG TGCCTTTCTC CACCGACTGC GAGGACTCAG ACATGCTACA TACGCCCGGT
GCTGCCTCCG ACAGGTCTGA GCATGACCTG ACCCCCATGG ACGGGGAGAC CTTCAGCCAG
CACCTGCAGG CCGTGAAGAT CCTCGCCGTC AGAGACCTCA TTTGGGTCCC CAGGCGCGGT
GGAGATGTTA TCGTCATTGG CCTGGAGAAG GATTCTGGCG CCCAGCGGGG CCGAGTCATT
GCCGTCTTAA AAGCCCGAGA GCTGACTCCG CATGGGGTGC TGGTGGATGC TGCCGTGGTG
GCAAAGGACA CTGTTGTGTG CACCTTTGAA AATGAAAACA CAGAGTGGTG CCTGGCCGTC
TGGAGGGGCT GGGGCGCCAG GGAGTTCGAC ATTTTCTACC AGTCCTACGA GGAGCTGGGC
CGGCTGGAGG CTTGCACTCG CAAGAGAAGG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48565
Clone ID Related Accession (Same CDS sequence) XM_005254979.3 , XM_011522018.2 , XM_011522017.2
Accession Version XM_011522018.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGCCACTG TCAACCAGTG CTTCTTCACG GACAAGGCCC TGTACGTGGT GGTCTGGAAC 
CTGGCGCTGG GGGAGGAGGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATCGAGGCC
AAGGCCCCAA ACGCCGTGGT GCTGGTGGTC GGGACGCACC TGGATTTAAT TGAAGCCAAG
TTCCGTGTGG AAAGGATTGC AACGCTGCGT GCCTATGTGC TGGCACTCTG CCGCTCCCCC
TCCGGCTCCA GGGCCACAGG CTTCCCAGAC ATCACCTTCA AACACTTACA TGAGATTTCC
TGCAAGAGCC TGGAAGGTCA GGAAGGGCTG CGACAGCTGA TTTTCCACGT CACGTGCAGC
ATGAAGGACG TGGGCAGCAC CATCGGCTGC CAGCGACTGG CAGGGCGGCT GATCCCCAGG
AGCTACCTGA GCCTGCAGGA GGCCGTGCTG GCAGAGCAGC AGCGCCGCAG CCGGGACGAC
GACGTGCAGT ACCTGACGGA CAGGCAGCTG GAGCAGCTGG TGGAGCAGAC GCCCGACAAC
GACATCAAGG ACTACGAGGA CCTGCAGTCA GCCATCAGCT TCCTCATAGA AACCGGCACC
CTGCTCCATT TCCCGGACAC CAGCCACGGC CTGAGGAACC TCTACTTCCT CGACCCTATT
TGGCTCTCCG AATGTCTGCA GAGGATCTTT AATATTAAGG GCTCTCGGTC AGTGGCCAAG
AATGGGGTGA TCAGAGCAGA AGACCTCAGG ATGCTGCTGG TGGGGACTGG CTTCACGCAG
CAGACGGAAG AGCAGTACTT CCAGTTCCTG GCCAAGTTTG AGATCGCCCT GCCCGTCGCC
AATGACAGCT ACCTCCTGCC CCATCTCCTT CCATCTAAAC CTGGCCTGGA CACCCACGGT
ATGCGGCACC CCACAGCCAA CACCATTCAG AGGGTATTTA AGATGAGCTT CGTTCCCGTT
GGCTTCTGGC AAAGGTTTAT AGCACGGATG CTGATCAGCC TGGCGGAGAT GGACCTGCAG
CTTTTTGAAA ACAAGAAGAA TACTAAAAGC AGGAACAGGA AAGTCACCAT TTACAGTTTT
ACAGGAAACC AGAGAAATCG CTGTAGCACA TTCAGAGTGA AAAGAAATCA GACCATCTAT
TGGCAGGAAG GGCTCCTGGT CACTTTTGAT GGGGGCTACC TCAGTGTGGA ATCTTCCGAC
GTGAACTGGA AAAAGAAGAA AAGCGGAGGA ATGAAAATTG TTTGCCAATC AGAAGTGAGG
GACTTCTCAG CCATGGCTTT CATCACGGAC CACGTCAATT CCTTGATTGA TCAGTGGTTT
CCCGCCCTGA CAGCCACAGA GAGCGACGGG ACGCCACTCA TGGAGCAGTA CGTGCCCTGC
CCGGTCTGCG AGACAGCCTG GGCCCAGCAC ACGGACCCCA GTGAGAAATC AGAGGATGTG
CAGTACTTCG ACATGGAAGA CTGTGTCCTG ACGGCCATCG AGCGGGACTT CATCTCCTGC
CCCAGACACC CGGACCTCCC CGTGCCGCTG CAGGAGCTGG TCCCTGAACT GTTCATGACC
GACTTCCCGG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACAGCGA GGACGAGGGC
AGCGTCCTGG GCCAGGGCGG CAGTGGCACC GTCATCTACC GGGCCCGGTA CCAGGGCCAG
CCTGTGGCCG TCAAGCGCTT CCACATCAAA AAATTCAAGA ACTTTGCTAA CGTACCGGCA
GACACCATGC TGAGGCACCT GCGGGCCACC GATGCCATGA AGAACTTCTC CGAGTTCCGG
CAGGAGGCCA GCATGCTGCA CGCGCTGCAG CACCCCTGCA TCGTGGCGCT CATCGGCATC
AGCATCCACC CGCTCTGCTT CGCCCTGGAG CTCGCGCCGC TCAGCAGCCT CAACACCGTG
CTGTCCGAGA ACGCCAGAGA TTCTTCCTTT ATACCCCTGG GACACATGCT CACCCAAAAA
ATAGCCTACC AGATCGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT
GACCTGAAGT CGGACAACAT TCTGGTGTGG TCCCTTGACG TCAAGGAGCA CATCAACATC
AAGCTATCTG ACTACGGGAT TTCGAGGCAG TCATTCCATG AGGGCGCCCT AGGCGTGGAG
GGCACTCCTG GCTACCAGGC CCCAGAGATC AGGCCTCGCA TTGTATATGA TGAGAAGGTA
GATATGTTCT CCTATGGAAT GGTGCTCTAC GAGTTGCTGT CAGGACAGCG CCCTGCACTG
GGCCACCACC AGCTCCAGAT TGCCAAGAAG CTGTCCAAGG GCATCCGCCC GGTTCTGGGG
CAGCCGGAGG AAGTGCAGTT CCGGCGACTG CAGGCGCTCA TGATGGAGTG CTGGGACACT
AAGCCAGAGA AGCGACCGCT GGCCCTGTCG GTGGTGAGCC AGATGAAGGA CCCGACTTTT
GCCACCTTCA TGTATGAACT GTGCTGTGGG AAGCAGACAG CCTTCTTCTC ATCCCAGGGC
CAGGAGTACA CCGTGGTGTT TTGGGATGGA AAAGAGGAGT CCAGGAACTA CACGGTGGTG
AACACAGAGA AGGGCCTCAT GGAGGTGCAG AGGATGTGCT GCCCTGGGAT GAAGGTGAGC
TGCCAGCTCC AGGTCCAGAG ATCCCTGTGG ACAGCCACCG AGGACCAGAA AATCTACATC
TACACCCTCA AGGGCATGTG CCCCTTAAAC ACACCCCAAC AGGCCTTGGA TACTCCAGCT
GTCGTCACCT GCTTCTTGGC CGTGCCTGTT ATTAAAAAGA ATTCCTACCT GGTCTTAGCG
GGCCTCGCCG ATGGGCTTGT GGCTGTGTTT CCCGTGGTGC GGGGCACCCC AAAGGACAGC
TGCTCCTACC TGTGCTCACA CACAGCCAAC AGGTCCAAGT TCAGCATCGC GGATGAAGAC
GCACGGCAGA ACCCCTACCC AGTGAAGGCC ATGGAGGTGG TCAACAGCGG CTCTGAGGTC
TGGTACAGCA ATGGGCCGGG CCTCCTTGTC ATCGACTGTG CCTCCCTGGA GATCTGCAGG
CGGCTGGAGC CCTACATGGC CCCCTCCATG GTTACGTCAG TCGTGTGCAG CTCTGAGGGC
AGAGGGGAGG AGGTCGTCTG GTGCCTGGAT GACAAGGCCA ACTCCTTGGT GATGTACCAC
TCCACCACCT ACCAGCTGTG TGCCCGGTAC TTCTGCGGGG TCCCCAGCCC CCTCAGGGAC
ATGTTTCCCG TGCGGCCCTT GGACACGGAA CCCCCGGCAG CCAGCCACAC GGCCAACCCA
AAGGTGCCTG AGGGGGACTC CATCGCGGAC GTGAGCATCA TGTACAGTGA GGAGCTGGGC
ACGCAGATCC TGATCCACCA GGAATCACTC ACTGACTACT GCTCCATGTC CTCCTACTCC
TCATCCCCAC CCCGCCAGGC TGCCAGGTCC CCCTCAAGCC TCCCCAGCTC CCCAGCAAGT
TCTTCCAGTG TGCCTTTCTC CACCGACTGC GAGGACTCAG ACATGCTACA TACGCCCGGT
GCTGCCTCCG ACAGGTCTGA GCATGACCTG ACCCCCATGG ACGGGGAGAC CTTCAGCCAG
CACCTGCAGG CCGTGAAGAT CCTCGCCGTC AGAGACCTCA TTTGGGTCCC CAGGCGCGGT
GGAGATGTTA TCGTCATTGG CCTGGAGAAG GATTCTGGCG CCCAGCGGGG CCGAGTCATT
GCCGTCTTAA AAGCCCGAGA GCTGACTCCG CATGGGGTGC TGGTGGATGC TGCCGTGGTG
GCAAAGGACA CTGTTGTGTG CACCTTTGAA AATGAAAACA CAGAGTGGTG CCTGGCCGTC
TGGAGGGGCT GGGGCGCCAG GGAGTTCGAC ATTTTCTACC AGTCCTACGA GGAGCTGGGC
CGGCTGGAGG CTTGCACTCG CAAGAGAAGG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520320.1
CDS135..4067
Misc Feature(1)<150..482
Misc Feature(2)288..299
Misc Feature(3)432..440
Misc Feature(4)549..1127
Misc Feature(5)1752..2552
Misc Feature(6)1761..2588
Misc Feature(7)order(1761..1775,1785..1787,1821..1823,1827..1829,1977..1979,2019..2030,2040..2042,2046..2048,2175..2177,2181..2183,2187..2192,2196..2198,2244..2246,2253..2255,2295..2306)
Misc Feature(8)order(1761..1775,1785..1787,1821..1823,1827..1829,1977..1979,2019..2030,2040..2042,2175..2177,2181..2183,2187..2192,2196..2198,2244..2246)
Misc Feature(9)order(1773..1775,2040..2042,2046..2048,2175..2177,2181..2183,2187..2189,2253..2255,2295..2306)
Misc Feature(10)2241..2306
Translation

Target ORF information:

RefSeq Version XM_011522018.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522018.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGCCACTG TCAACCAGTG CTTCTTCACG GACAAGGCCC TGTACGTGGT GGTCTGGAAC 
CTGGCGCTGG GGGAGGAGGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATCGAGGCC
AAGGCCCCAA ACGCCGTGGT GCTGGTGGTC GGGACGCACC TGGATTTAAT TGAAGCCAAG
TTCCGTGTGG AAAGGATTGC AACGCTGCGT GCCTATGTGC TGGCACTCTG CCGCTCCCCC
TCCGGCTCCA GGGCCACAGG CTTCCCAGAC ATCACCTTCA AACACTTACA TGAGATTTCC
TGCAAGAGCC TGGAAGGTCA GGAAGGGCTG CGACAGCTGA TTTTCCACGT CACGTGCAGC
ATGAAGGACG TGGGCAGCAC CATCGGCTGC CAGCGACTGG CAGGGCGGCT GATCCCCAGG
AGCTACCTGA GCCTGCAGGA GGCCGTGCTG GCAGAGCAGC AGCGCCGCAG CCGGGACGAC
GACGTGCAGT ACCTGACGGA CAGGCAGCTG GAGCAGCTGG TGGAGCAGAC GCCCGACAAC
GACATCAAGG ACTACGAGGA CCTGCAGTCA GCCATCAGCT TCCTCATAGA AACCGGCACC
CTGCTCCATT TCCCGGACAC CAGCCACGGC CTGAGGAACC TCTACTTCCT CGACCCTATT
TGGCTCTCCG AATGTCTGCA GAGGATCTTT AATATTAAGG GCTCTCGGTC AGTGGCCAAG
AATGGGGTGA TCAGAGCAGA AGACCTCAGG ATGCTGCTGG TGGGGACTGG CTTCACGCAG
CAGACGGAAG AGCAGTACTT CCAGTTCCTG GCCAAGTTTG AGATCGCCCT GCCCGTCGCC
AATGACAGCT ACCTCCTGCC CCATCTCCTT CCATCTAAAC CTGGCCTGGA CACCCACGGT
ATGCGGCACC CCACAGCCAA CACCATTCAG AGGGTATTTA AGATGAGCTT CGTTCCCGTT
GGCTTCTGGC AAAGGTTTAT AGCACGGATG CTGATCAGCC TGGCGGAGAT GGACCTGCAG
CTTTTTGAAA ACAAGAAGAA TACTAAAAGC AGGAACAGGA AAGTCACCAT TTACAGTTTT
ACAGGAAACC AGAGAAATCG CTGTAGCACA TTCAGAGTGA AAAGAAATCA GACCATCTAT
TGGCAGGAAG GGCTCCTGGT CACTTTTGAT GGGGGCTACC TCAGTGTGGA ATCTTCCGAC
GTGAACTGGA AAAAGAAGAA AAGCGGAGGA ATGAAAATTG TTTGCCAATC AGAAGTGAGG
GACTTCTCAG CCATGGCTTT CATCACGGAC CACGTCAATT CCTTGATTGA TCAGTGGTTT
CCCGCCCTGA CAGCCACAGA GAGCGACGGG ACGCCACTCA TGGAGCAGTA CGTGCCCTGC
CCGGTCTGCG AGACAGCCTG GGCCCAGCAC ACGGACCCCA GTGAGAAATC AGAGGATGTG
CAGTACTTCG ACATGGAAGA CTGTGTCCTG ACGGCCATCG AGCGGGACTT CATCTCCTGC
CCCAGACACC CGGACCTCCC CGTGCCGCTG CAGGAGCTGG TCCCTGAACT GTTCATGACC
GACTTCCCGG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACAGCGA GGACGAGGGC
AGCGTCCTGG GCCAGGGCGG CAGTGGCACC GTCATCTACC GGGCCCGGTA CCAGGGCCAG
CCTGTGGCCG TCAAGCGCTT CCACATCAAA AAATTCAAGA ACTTTGCTAA CGTACCGGCA
GACACCATGC TGAGGCACCT GCGGGCCACC GATGCCATGA AGAACTTCTC CGAGTTCCGG
CAGGAGGCCA GCATGCTGCA CGCGCTGCAG CACCCCTGCA TCGTGGCGCT CATCGGCATC
AGCATCCACC CGCTCTGCTT CGCCCTGGAG CTCGCGCCGC TCAGCAGCCT CAACACCGTG
CTGTCCGAGA ACGCCAGAGA TTCTTCCTTT ATACCCCTGG GACACATGCT CACCCAAAAA
ATAGCCTACC AGATCGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT
GACCTGAAGT CGGACAACAT TCTGGTGTGG TCCCTTGACG TCAAGGAGCA CATCAACATC
AAGCTATCTG ACTACGGGAT TTCGAGGCAG TCATTCCATG AGGGCGCCCT AGGCGTGGAG
GGCACTCCTG GCTACCAGGC CCCAGAGATC AGGCCTCGCA TTGTATATGA TGAGAAGGTA
GATATGTTCT CCTATGGAAT GGTGCTCTAC GAGTTGCTGT CAGGACAGCG CCCTGCACTG
GGCCACCACC AGCTCCAGAT TGCCAAGAAG CTGTCCAAGG GCATCCGCCC GGTTCTGGGG
CAGCCGGAGG AAGTGCAGTT CCGGCGACTG CAGGCGCTCA TGATGGAGTG CTGGGACACT
AAGCCAGAGA AGCGACCGCT GGCCCTGTCG GTGGTGAGCC AGATGAAGGA CCCGACTTTT
GCCACCTTCA TGTATGAACT GTGCTGTGGG AAGCAGACAG CCTTCTTCTC ATCCCAGGGC
CAGGAGTACA CCGTGGTGTT TTGGGATGGA AAAGAGGAGT CCAGGAACTA CACGGTGGTG
AACACAGAGA AGGGCCTCAT GGAGGTGCAG AGGATGTGCT GCCCTGGGAT GAAGGTGAGC
TGCCAGCTCC AGGTCCAGAG ATCCCTGTGG ACAGCCACCG AGGACCAGAA AATCTACATC
TACACCCTCA AGGGCATGTG CCCCTTAAAC ACACCCCAAC AGGCCTTGGA TACTCCAGCT
GTCGTCACCT GCTTCTTGGC CGTGCCTGTT ATTAAAAAGA ATTCCTACCT GGTCTTAGCG
GGCCTCGCCG ATGGGCTTGT GGCTGTGTTT CCCGTGGTGC GGGGCACCCC AAAGGACAGC
TGCTCCTACC TGTGCTCACA CACAGCCAAC AGGTCCAAGT TCAGCATCGC GGATGAAGAC
GCACGGCAGA ACCCCTACCC AGTGAAGGCC ATGGAGGTGG TCAACAGCGG CTCTGAGGTC
TGGTACAGCA ATGGGCCGGG CCTCCTTGTC ATCGACTGTG CCTCCCTGGA GATCTGCAGG
CGGCTGGAGC CCTACATGGC CCCCTCCATG GTTACGTCAG TCGTGTGCAG CTCTGAGGGC
AGAGGGGAGG AGGTCGTCTG GTGCCTGGAT GACAAGGCCA ACTCCTTGGT GATGTACCAC
TCCACCACCT ACCAGCTGTG TGCCCGGTAC TTCTGCGGGG TCCCCAGCCC CCTCAGGGAC
ATGTTTCCCG TGCGGCCCTT GGACACGGAA CCCCCGGCAG CCAGCCACAC GGCCAACCCA
AAGGTGCCTG AGGGGGACTC CATCGCGGAC GTGAGCATCA TGTACAGTGA GGAGCTGGGC
ACGCAGATCC TGATCCACCA GGAATCACTC ACTGACTACT GCTCCATGTC CTCCTACTCC
TCATCCCCAC CCCGCCAGGC TGCCAGGTCC CCCTCAAGCC TCCCCAGCTC CCCAGCAAGT
TCTTCCAGTG TGCCTTTCTC CACCGACTGC GAGGACTCAG ACATGCTACA TACGCCCGGT
GCTGCCTCCG ACAGGTCTGA GCATGACCTG ACCCCCATGG ACGGGGAGAC CTTCAGCCAG
CACCTGCAGG CCGTGAAGAT CCTCGCCGTC AGAGACCTCA TTTGGGTCCC CAGGCGCGGT
GGAGATGTTA TCGTCATTGG CCTGGAGAAG GATTCTGGCG CCCAGCGGGG CCGAGTCATT
GCCGTCTTAA AAGCCCGAGA GCTGACTCCG CATGGGGTGC TGGTGGATGC TGCCGTGGTG
GCAAAGGACA CTGTTGTGTG CACCTTTGAA AATGAAAACA CAGAGTGGTG CCTGGCCGTC
TGGAGGGGCT GGGGCGCCAG GGAGTTCGAC ATTTTCTACC AGTCCTACGA GGAGCTGGGC
CGGCTGGAGG CTTGCACTCG CAAGAGAAGG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu48565
Clone ID Related Accession (Same CDS sequence) XM_005254979.3 , XM_011522018.2 , XM_011522017.2
Accession Version XM_011522017.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGCCACTG TCAACCAGTG CTTCTTCACG GACAAGGCCC TGTACGTGGT GGTCTGGAAC 
CTGGCGCTGG GGGAGGAGGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATCGAGGCC
AAGGCCCCAA ACGCCGTGGT GCTGGTGGTC GGGACGCACC TGGATTTAAT TGAAGCCAAG
TTCCGTGTGG AAAGGATTGC AACGCTGCGT GCCTATGTGC TGGCACTCTG CCGCTCCCCC
TCCGGCTCCA GGGCCACAGG CTTCCCAGAC ATCACCTTCA AACACTTACA TGAGATTTCC
TGCAAGAGCC TGGAAGGTCA GGAAGGGCTG CGACAGCTGA TTTTCCACGT CACGTGCAGC
ATGAAGGACG TGGGCAGCAC CATCGGCTGC CAGCGACTGG CAGGGCGGCT GATCCCCAGG
AGCTACCTGA GCCTGCAGGA GGCCGTGCTG GCAGAGCAGC AGCGCCGCAG CCGGGACGAC
GACGTGCAGT ACCTGACGGA CAGGCAGCTG GAGCAGCTGG TGGAGCAGAC GCCCGACAAC
GACATCAAGG ACTACGAGGA CCTGCAGTCA GCCATCAGCT TCCTCATAGA AACCGGCACC
CTGCTCCATT TCCCGGACAC CAGCCACGGC CTGAGGAACC TCTACTTCCT CGACCCTATT
TGGCTCTCCG AATGTCTGCA GAGGATCTTT AATATTAAGG GCTCTCGGTC AGTGGCCAAG
AATGGGGTGA TCAGAGCAGA AGACCTCAGG ATGCTGCTGG TGGGGACTGG CTTCACGCAG
CAGACGGAAG AGCAGTACTT CCAGTTCCTG GCCAAGTTTG AGATCGCCCT GCCCGTCGCC
AATGACAGCT ACCTCCTGCC CCATCTCCTT CCATCTAAAC CTGGCCTGGA CACCCACGGT
ATGCGGCACC CCACAGCCAA CACCATTCAG AGGGTATTTA AGATGAGCTT CGTTCCCGTT
GGCTTCTGGC AAAGGTTTAT AGCACGGATG CTGATCAGCC TGGCGGAGAT GGACCTGCAG
CTTTTTGAAA ACAAGAAGAA TACTAAAAGC AGGAACAGGA AAGTCACCAT TTACAGTTTT
ACAGGAAACC AGAGAAATCG CTGTAGCACA TTCAGAGTGA AAAGAAATCA GACCATCTAT
TGGCAGGAAG GGCTCCTGGT CACTTTTGAT GGGGGCTACC TCAGTGTGGA ATCTTCCGAC
GTGAACTGGA AAAAGAAGAA AAGCGGAGGA ATGAAAATTG TTTGCCAATC AGAAGTGAGG
GACTTCTCAG CCATGGCTTT CATCACGGAC CACGTCAATT CCTTGATTGA TCAGTGGTTT
CCCGCCCTGA CAGCCACAGA GAGCGACGGG ACGCCACTCA TGGAGCAGTA CGTGCCCTGC
CCGGTCTGCG AGACAGCCTG GGCCCAGCAC ACGGACCCCA GTGAGAAATC AGAGGATGTG
CAGTACTTCG ACATGGAAGA CTGTGTCCTG ACGGCCATCG AGCGGGACTT CATCTCCTGC
CCCAGACACC CGGACCTCCC CGTGCCGCTG CAGGAGCTGG TCCCTGAACT GTTCATGACC
GACTTCCCGG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACAGCGA GGACGAGGGC
AGCGTCCTGG GCCAGGGCGG CAGTGGCACC GTCATCTACC GGGCCCGGTA CCAGGGCCAG
CCTGTGGCCG TCAAGCGCTT CCACATCAAA AAATTCAAGA ACTTTGCTAA CGTACCGGCA
GACACCATGC TGAGGCACCT GCGGGCCACC GATGCCATGA AGAACTTCTC CGAGTTCCGG
CAGGAGGCCA GCATGCTGCA CGCGCTGCAG CACCCCTGCA TCGTGGCGCT CATCGGCATC
AGCATCCACC CGCTCTGCTT CGCCCTGGAG CTCGCGCCGC TCAGCAGCCT CAACACCGTG
CTGTCCGAGA ACGCCAGAGA TTCTTCCTTT ATACCCCTGG GACACATGCT CACCCAAAAA
ATAGCCTACC AGATCGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT
GACCTGAAGT CGGACAACAT TCTGGTGTGG TCCCTTGACG TCAAGGAGCA CATCAACATC
AAGCTATCTG ACTACGGGAT TTCGAGGCAG TCATTCCATG AGGGCGCCCT AGGCGTGGAG
GGCACTCCTG GCTACCAGGC CCCAGAGATC AGGCCTCGCA TTGTATATGA TGAGAAGGTA
GATATGTTCT CCTATGGAAT GGTGCTCTAC GAGTTGCTGT CAGGACAGCG CCCTGCACTG
GGCCACCACC AGCTCCAGAT TGCCAAGAAG CTGTCCAAGG GCATCCGCCC GGTTCTGGGG
CAGCCGGAGG AAGTGCAGTT CCGGCGACTG CAGGCGCTCA TGATGGAGTG CTGGGACACT
AAGCCAGAGA AGCGACCGCT GGCCCTGTCG GTGGTGAGCC AGATGAAGGA CCCGACTTTT
GCCACCTTCA TGTATGAACT GTGCTGTGGG AAGCAGACAG CCTTCTTCTC ATCCCAGGGC
CAGGAGTACA CCGTGGTGTT TTGGGATGGA AAAGAGGAGT CCAGGAACTA CACGGTGGTG
AACACAGAGA AGGGCCTCAT GGAGGTGCAG AGGATGTGCT GCCCTGGGAT GAAGGTGAGC
TGCCAGCTCC AGGTCCAGAG ATCCCTGTGG ACAGCCACCG AGGACCAGAA AATCTACATC
TACACCCTCA AGGGCATGTG CCCCTTAAAC ACACCCCAAC AGGCCTTGGA TACTCCAGCT
GTCGTCACCT GCTTCTTGGC CGTGCCTGTT ATTAAAAAGA ATTCCTACCT GGTCTTAGCG
GGCCTCGCCG ATGGGCTTGT GGCTGTGTTT CCCGTGGTGC GGGGCACCCC AAAGGACAGC
TGCTCCTACC TGTGCTCACA CACAGCCAAC AGGTCCAAGT TCAGCATCGC GGATGAAGAC
GCACGGCAGA ACCCCTACCC AGTGAAGGCC ATGGAGGTGG TCAACAGCGG CTCTGAGGTC
TGGTACAGCA ATGGGCCGGG CCTCCTTGTC ATCGACTGTG CCTCCCTGGA GATCTGCAGG
CGGCTGGAGC CCTACATGGC CCCCTCCATG GTTACGTCAG TCGTGTGCAG CTCTGAGGGC
AGAGGGGAGG AGGTCGTCTG GTGCCTGGAT GACAAGGCCA ACTCCTTGGT GATGTACCAC
TCCACCACCT ACCAGCTGTG TGCCCGGTAC TTCTGCGGGG TCCCCAGCCC CCTCAGGGAC
ATGTTTCCCG TGCGGCCCTT GGACACGGAA CCCCCGGCAG CCAGCCACAC GGCCAACCCA
AAGGTGCCTG AGGGGGACTC CATCGCGGAC GTGAGCATCA TGTACAGTGA GGAGCTGGGC
ACGCAGATCC TGATCCACCA GGAATCACTC ACTGACTACT GCTCCATGTC CTCCTACTCC
TCATCCCCAC CCCGCCAGGC TGCCAGGTCC CCCTCAAGCC TCCCCAGCTC CCCAGCAAGT
TCTTCCAGTG TGCCTTTCTC CACCGACTGC GAGGACTCAG ACATGCTACA TACGCCCGGT
GCTGCCTCCG ACAGGTCTGA GCATGACCTG ACCCCCATGG ACGGGGAGAC CTTCAGCCAG
CACCTGCAGG CCGTGAAGAT CCTCGCCGTC AGAGACCTCA TTTGGGTCCC CAGGCGCGGT
GGAGATGTTA TCGTCATTGG CCTGGAGAAG GATTCTGGCG CCCAGCGGGG CCGAGTCATT
GCCGTCTTAA AAGCCCGAGA GCTGACTCCG CATGGGGTGC TGGTGGATGC TGCCGTGGTG
GCAAAGGACA CTGTTGTGTG CACCTTTGAA AATGAAAACA CAGAGTGGTG CCTGGCCGTC
TGGAGGGGCT GGGGCGCCAG GGAGTTCGAC ATTTTCTACC AGTCCTACGA GGAGCTGGGC
CGGCTGGAGG CTTGCACTCG CAAGAGAAGG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520319.1
CDS140..4072
Misc Feature(1)<155..487
Misc Feature(2)293..304
Misc Feature(3)437..445
Misc Feature(4)554..1132
Misc Feature(5)1757..2557
Misc Feature(6)1766..2593
Misc Feature(7)order(1766..1780,1790..1792,1826..1828,1832..1834,1982..1984,2024..2035,2045..2047,2051..2053,2180..2182,2186..2188,2192..2197,2201..2203,2249..2251,2258..2260,2300..2311)
Misc Feature(8)order(1766..1780,1790..1792,1826..1828,1832..1834,1982..1984,2024..2035,2045..2047,2180..2182,2186..2188,2192..2197,2201..2203,2249..2251)
Misc Feature(9)order(1778..1780,2045..2047,2051..2053,2180..2182,2186..2188,2192..2194,2258..2260,2300..2311)
Misc Feature(10)2246..2311
Translation

Target ORF information:

RefSeq Version XM_011522017.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522017.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGGCCACTG TCAACCAGTG CTTCTTCACG GACAAGGCCC TGTACGTGGT GGTCTGGAAC 
CTGGCGCTGG GGGAGGAGGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATCGAGGCC
AAGGCCCCAA ACGCCGTGGT GCTGGTGGTC GGGACGCACC TGGATTTAAT TGAAGCCAAG
TTCCGTGTGG AAAGGATTGC AACGCTGCGT GCCTATGTGC TGGCACTCTG CCGCTCCCCC
TCCGGCTCCA GGGCCACAGG CTTCCCAGAC ATCACCTTCA AACACTTACA TGAGATTTCC
TGCAAGAGCC TGGAAGGTCA GGAAGGGCTG CGACAGCTGA TTTTCCACGT CACGTGCAGC
ATGAAGGACG TGGGCAGCAC CATCGGCTGC CAGCGACTGG CAGGGCGGCT GATCCCCAGG
AGCTACCTGA GCCTGCAGGA GGCCGTGCTG GCAGAGCAGC AGCGCCGCAG CCGGGACGAC
GACGTGCAGT ACCTGACGGA CAGGCAGCTG GAGCAGCTGG TGGAGCAGAC GCCCGACAAC
GACATCAAGG ACTACGAGGA CCTGCAGTCA GCCATCAGCT TCCTCATAGA AACCGGCACC
CTGCTCCATT TCCCGGACAC CAGCCACGGC CTGAGGAACC TCTACTTCCT CGACCCTATT
TGGCTCTCCG AATGTCTGCA GAGGATCTTT AATATTAAGG GCTCTCGGTC AGTGGCCAAG
AATGGGGTGA TCAGAGCAGA AGACCTCAGG ATGCTGCTGG TGGGGACTGG CTTCACGCAG
CAGACGGAAG AGCAGTACTT CCAGTTCCTG GCCAAGTTTG AGATCGCCCT GCCCGTCGCC
AATGACAGCT ACCTCCTGCC CCATCTCCTT CCATCTAAAC CTGGCCTGGA CACCCACGGT
ATGCGGCACC CCACAGCCAA CACCATTCAG AGGGTATTTA AGATGAGCTT CGTTCCCGTT
GGCTTCTGGC AAAGGTTTAT AGCACGGATG CTGATCAGCC TGGCGGAGAT GGACCTGCAG
CTTTTTGAAA ACAAGAAGAA TACTAAAAGC AGGAACAGGA AAGTCACCAT TTACAGTTTT
ACAGGAAACC AGAGAAATCG CTGTAGCACA TTCAGAGTGA AAAGAAATCA GACCATCTAT
TGGCAGGAAG GGCTCCTGGT CACTTTTGAT GGGGGCTACC TCAGTGTGGA ATCTTCCGAC
GTGAACTGGA AAAAGAAGAA AAGCGGAGGA ATGAAAATTG TTTGCCAATC AGAAGTGAGG
GACTTCTCAG CCATGGCTTT CATCACGGAC CACGTCAATT CCTTGATTGA TCAGTGGTTT
CCCGCCCTGA CAGCCACAGA GAGCGACGGG ACGCCACTCA TGGAGCAGTA CGTGCCCTGC
CCGGTCTGCG AGACAGCCTG GGCCCAGCAC ACGGACCCCA GTGAGAAATC AGAGGATGTG
CAGTACTTCG ACATGGAAGA CTGTGTCCTG ACGGCCATCG AGCGGGACTT CATCTCCTGC
CCCAGACACC CGGACCTCCC CGTGCCGCTG CAGGAGCTGG TCCCTGAACT GTTCATGACC
GACTTCCCGG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACAGCGA GGACGAGGGC
AGCGTCCTGG GCCAGGGCGG CAGTGGCACC GTCATCTACC GGGCCCGGTA CCAGGGCCAG
CCTGTGGCCG TCAAGCGCTT CCACATCAAA AAATTCAAGA ACTTTGCTAA CGTACCGGCA
GACACCATGC TGAGGCACCT GCGGGCCACC GATGCCATGA AGAACTTCTC CGAGTTCCGG
CAGGAGGCCA GCATGCTGCA CGCGCTGCAG CACCCCTGCA TCGTGGCGCT CATCGGCATC
AGCATCCACC CGCTCTGCTT CGCCCTGGAG CTCGCGCCGC TCAGCAGCCT CAACACCGTG
CTGTCCGAGA ACGCCAGAGA TTCTTCCTTT ATACCCCTGG GACACATGCT CACCCAAAAA
ATAGCCTACC AGATCGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT
GACCTGAAGT CGGACAACAT TCTGGTGTGG TCCCTTGACG TCAAGGAGCA CATCAACATC
AAGCTATCTG ACTACGGGAT TTCGAGGCAG TCATTCCATG AGGGCGCCCT AGGCGTGGAG
GGCACTCCTG GCTACCAGGC CCCAGAGATC AGGCCTCGCA TTGTATATGA TGAGAAGGTA
GATATGTTCT CCTATGGAAT GGTGCTCTAC GAGTTGCTGT CAGGACAGCG CCCTGCACTG
GGCCACCACC AGCTCCAGAT TGCCAAGAAG CTGTCCAAGG GCATCCGCCC GGTTCTGGGG
CAGCCGGAGG AAGTGCAGTT CCGGCGACTG CAGGCGCTCA TGATGGAGTG CTGGGACACT
AAGCCAGAGA AGCGACCGCT GGCCCTGTCG GTGGTGAGCC AGATGAAGGA CCCGACTTTT
GCCACCTTCA TGTATGAACT GTGCTGTGGG AAGCAGACAG CCTTCTTCTC ATCCCAGGGC
CAGGAGTACA CCGTGGTGTT TTGGGATGGA AAAGAGGAGT CCAGGAACTA CACGGTGGTG
AACACAGAGA AGGGCCTCAT GGAGGTGCAG AGGATGTGCT GCCCTGGGAT GAAGGTGAGC
TGCCAGCTCC AGGTCCAGAG ATCCCTGTGG ACAGCCACCG AGGACCAGAA AATCTACATC
TACACCCTCA AGGGCATGTG CCCCTTAAAC ACACCCCAAC AGGCCTTGGA TACTCCAGCT
GTCGTCACCT GCTTCTTGGC CGTGCCTGTT ATTAAAAAGA ATTCCTACCT GGTCTTAGCG
GGCCTCGCCG ATGGGCTTGT GGCTGTGTTT CCCGTGGTGC GGGGCACCCC AAAGGACAGC
TGCTCCTACC TGTGCTCACA CACAGCCAAC AGGTCCAAGT TCAGCATCGC GGATGAAGAC
GCACGGCAGA ACCCCTACCC AGTGAAGGCC ATGGAGGTGG TCAACAGCGG CTCTGAGGTC
TGGTACAGCA ATGGGCCGGG CCTCCTTGTC ATCGACTGTG CCTCCCTGGA GATCTGCAGG
CGGCTGGAGC CCTACATGGC CCCCTCCATG GTTACGTCAG TCGTGTGCAG CTCTGAGGGC
AGAGGGGAGG AGGTCGTCTG GTGCCTGGAT GACAAGGCCA ACTCCTTGGT GATGTACCAC
TCCACCACCT ACCAGCTGTG TGCCCGGTAC TTCTGCGGGG TCCCCAGCCC CCTCAGGGAC
ATGTTTCCCG TGCGGCCCTT GGACACGGAA CCCCCGGCAG CCAGCCACAC GGCCAACCCA
AAGGTGCCTG AGGGGGACTC CATCGCGGAC GTGAGCATCA TGTACAGTGA GGAGCTGGGC
ACGCAGATCC TGATCCACCA GGAATCACTC ACTGACTACT GCTCCATGTC CTCCTACTCC
TCATCCCCAC CCCGCCAGGC TGCCAGGTCC CCCTCAAGCC TCCCCAGCTC CCCAGCAAGT
TCTTCCAGTG TGCCTTTCTC CACCGACTGC GAGGACTCAG ACATGCTACA TACGCCCGGT
GCTGCCTCCG ACAGGTCTGA GCATGACCTG ACCCCCATGG ACGGGGAGAC CTTCAGCCAG
CACCTGCAGG CCGTGAAGAT CCTCGCCGTC AGAGACCTCA TTTGGGTCCC CAGGCGCGGT
GGAGATGTTA TCGTCATTGG CCTGGAGAAG GATTCTGGCG CCCAGCGGGG CCGAGTCATT
GCCGTCTTAA AAGCCCGAGA GCTGACTCCG CATGGGGTGC TGGTGGATGC TGCCGTGGTG
GCAAAGGACA CTGTTGTGTG CACCTTTGAA AATGAAAACA CAGAGTGGTG CCTGGCCGTC
TGGAGGGGCT GGGGCGCCAG GGAGTTCGAC ATTTTCTACC AGTCCTACGA GGAGCTGGGC
CGGCTGGAGG CTTGCACTCG CAAGAGAAGG TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72035
Clone ID Related Accession (Same CDS sequence) XM_011522019.2
Accession Version XM_011522019.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522019.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGTGTGG AATCTTCCGA CGTGAACTGG AAAAAGAAGA AAAGCGGAGG
AATGAAAATT GTTTGCCAAT CAGAAGTGAG GGACTTCTCA GCCATGGCTT TCATCACGGA
CCACGTCAAT TCCTTGATTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520321.1
CDS558..3818
Misc Feature(1)840..1199
Misc Feature(2)933..1220
Misc Feature(3)order(933..935,942..950,954..959,969..971,978..980,1005..1007,1011..1013,1017..1019,1029..1034,1041..1049,1053..1058,1068..1070,1080..1082,1113..1115,1119..1121,1140..1142,1152..1157,1164..1172,1176..1181,1191..1193,1200..1202)
Misc Feature(4)1011..1115
Misc Feature(5)1119..1211
Misc Feature(6)<1197..1949
Misc Feature(7)1398..1466
Misc Feature(8)1467..1547
Misc Feature(9)1542..1805
Misc Feature(10)1548..1616
Misc Feature(11)1617..1700
Misc Feature(12)1698..>2405
Misc Feature(13)1701..1772
Misc Feature(14)1773..1910
Misc Feature(15)1911..1979
Misc Feature(16)1980..2051
Misc Feature(17)2052..2204
Misc Feature(18)2205..2273
Misc Feature(19)2472..3020
Misc Feature(20)2472..3017
Misc Feature(21)2490..2513
Misc Feature(22)order(2496..2516,2661..2663,2826..2831,2835..2837,2970..2978)
Misc Feature(23)2556..2558
Misc Feature(24)2577..2585
Misc Feature(25)2652..2663
Misc Feature(26)order(2658..2663,2709..2714)
Misc Feature(27)2826..2837
Misc Feature(28)2970..2978
Misc Feature(29)3087..3665
Translation

Target ORF information:

RefSeq Version XM_011522019.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522019.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGTGTGG AATCTTCCGA CGTGAACTGG AAAAAGAAGA AAAGCGGAGG
AATGAAAATT GTTTGCCAAT CAGAAGTGAG GGACTTCTCA GCCATGGCTT TCATCACGGA
CCACGTCAAT TCCTTGATTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu85662
Clone ID Related Accession (Same CDS sequence) XM_017022570.1
Accession Version XM_017022570.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTGGATTT CTCAGAAAAC GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATA 
AATGAAGATG GACTGAAAAC GAAGCGTATT GCCTTTTTCA CCACCAGAGG TCGCCAGCGC
TCCGGGACTG AGGCAGCAAG TGTGCTGGAA TTTCCGGCCT TCCTAAGTGA GTCTTTGGAA
GTCCTTTGCC TGAACGACAA CCACCTCGAC ACAGTCCCTC CCTCGGTTTG CCTACTGAAG
AGCTTATCAG AGCTCTACTT GGGAAACAAC CCTGGCCTCC GGGAGCTCCC TCCTGAGCTG
GGGCAGCTGG GCAACCTCTG GCAGCTGGAC ACTGAAGACC TGACCATCAG CAATGTGCCT
GCAGAAATCC AAAAAGAAGG CCCCAAAGCA ATGCTGTCTT ACCTGCGTGC TCAGCTGCGG
AAAGCGGAAA AGTGCAAGCT GATGAAGATG ATCATCGTGG GTCCCCCGCG CCAGGGCAAG
TCCACCCTCC TGGAGATCTT ACAGACGGGG AGGGCCCCCC AGGTGGTGCA TGGAGAGGCC
ACCATCAGGA CCACCAAGTG GGAGCTCCAG AGGCCGGCTG GCTCGAGAGC CAAGGTTGAG
TCCGTGGAGT TCAACGTCTG GGACATCGGG GGACCGGCCA GCATGGCCAC TGTCAACCAG
TGCTTCTTCA CGGACAAGGC CCTGTACGTG GTGGTCTGGA ACCTGGCGCT GGGGGAGGAG
GCCGTGGCCA ACCTCCAGTT CTGGCTGCTC AACATCGAGG CCAAGGCCCC AAACGCCGTG
GTGCTGGTGG TCGGGACGCA CCTGGATTTA ATTGAAGCCA AGTTCCGTGT GGAAAGGATT
GCAACGCTGC GTGCCTATGT GCTGGCACTC TGCCGCTCCC CCTCCGGCTC CAGGGCCACA
GGCTTCCCAG ACATCACCTT CAAACACTTA CATGAGATTT CCTGCAAGAG CCTGGAAGGT
CAGGAAGGGC TGCGACAGCT GATTTTCCAC GTCACGTGCA GCATGAAGGA CGTGGGCAGC
ACCATCGGCT GCCAGCGACT GGCAGGGCGG CTGATCCCCA GGAGCTACCT GAGCCTGCAG
GAGGCCGTGC TGGCAGAGCA GCAGCGCCGC AGCCGGGACG ACGACGTGCA GTACCTGACG
GACAGGCAGC TGGAGCAGCT GGTGGAGCAG ACGCCCGACA ACGACATCAA GGACTACGAG
GACCTGCAGT CAGCCATCAG CTTCCTCATA GAAACCGGCA CCCTGCTCCA TTTCCCGGAC
ACCAGCCACG GCCTGAGGAA CCTCTACTTC CTCGACCCTA TTTGGCTCTC CGAATGTCTG
CAGAGGATCT TTAATATTAA GGGCTCTCGG TCAGTGGCCA AGAATGGGGT GATCAGAGCA
GAAGACCTCA GGATGCTGCT GGTGGGGACT GGCTTCACGC AGCAGACGGA AGAGCAGTAC
TTCCAGTTCC TGGCCAAGTT TGAGATCGCC CTGCCCGTCG CCAATGACAG CTACCTCCTG
CCCCATCTCC TTCCATCTAA ACCTGGCCTG GACACCCACG GTATGCGGCA CCCCACAGCC
AACACCATTC AGAGGGTATT TAAGATGAGC TTCGTTCCCG TTGGCTTCTG GCAAAGGTTT
ATAGCACGGA TGCTGATCAG CCTGGCGGAG ATGGACCTGC AGCTTTTTGA AAACAAGAAG
AATACTAAAA GCAGGAACAG GAAAGTCACC ATTTACAGTT TTACAGGAAA CCAGAGAAAT
CGCTGTAGCA CATTCAGAGT GAAAAGAAAT CAGACCATCT ATTGGCAGGA AGGGCTCCTG
GTCACTTTTG ATGGGGGCTA CCTCAGTGTG GAATCTTCCG ACGTGAACTG GAAAAAGAAG
AAAAGCGGAG GAATGAAAAT TGTTTGCCAA TCAGAAGTGA GGGACTTCTC AGCCATGGCT
TTCATCACGG ACCACGTCAA TTCCTTGATT GATCAGTGGT TTCCCGCCCT GACAGCCACA
GAGAGCGACG GGACGCCACT CATGGAGCAG TACGTGCCCT GCCCGGTCTG CGAGACAGCC
TGGGCCCAGC ACACGGACCC CAGTGAGAAA TCAGAGGATG TGCAGTACTT CGACATGGAA
GACTGTGTCC TGACGGCCAT CGAGCGGGAC TTCATCTCCT GCCCCAGACA CCCGGACCTC
CCCGTGCCGC TGCAGGAGCT GGTCCCTGAA CTGTTCATGA CCGACTTCCC GGCCAGGCTC
TTCCTGGAGA ACAGCAAGCT GGAGCACAGC GAGGACGAGG GCAGCGTCCT GGGCCAGGGC
GGCAGTGGCA CCGTCATCTA CCGGGCCCGG TACCAGGGCC AGCCTGTGGC CGTCAAGCGC
TTCCACATCA AAAAATTCAA GAACTTTGCT AACGTACCGG CAGACACCAT GCTGAGGCAC
CTGCGGGCCA CCGATGCCAT GAAGAACTTC TCCGAGTTCC GGCAGGAGGC CAGCATGCTG
CACGCGCTGC AGCACCCCTG CATCGTGGCG CTCATCGGCA TCAGCATCCA CCCGCTCTGC
TTCGCCCTGG AGCTCGCGCC GCTCAGCAGC CTCAACACCG TGCTGTCCGA GAACGCCAGA
GATTCTTCCT TTATACCCCT GGGACACATG CTCACCCAAA AAATAGCCTA CCAGATCGCC
TCGGGCCTGG CCTACCTGCA CAAGAAAAAC ATCATCTTCT GTGACCTGAA GTCGGACAAC
ATTCTGGTGT GGTCCCTTGA CGTCAAGGAG CACATCAACA TCAAGCTATC TGACTACGGG
ATTTCGAGGC AGTCATTCCA TGAGGGCGCC CTAGGCGTGG AGGGCACTCC TGGCTACCAG
GCCCCAGAGA TCAGGCCTCG CATTGTATAT GATGAGAAGG TAGATATGTT CTCCTATGGA
ATGGTGCTCT ACGAGTTGCT GTCAGGACAG CGCCCTGCAC TGGGCCACCA CCAGCTCCAG
ATTGCCAAGA AGCTGTCCAA GGGCATCCGC CCGGTTCTGG GGCAGCCGGA GGAAGTGCAG
TTCCGGCGAC TGCAGGCGCT CATGATGGAG TGCTGGGACA CTAAGCCAGA GAAGCGACCG
CTGGCCCTGT CGGTGGTGAG CCAGATGAAG GACCCGACTT TTGCCACCTT CATGTATGAA
CTGTGCTGTG GGAAGCAGAC AGCCTTCTTC TCATCCCAGG GCCAGGAGTA CACCGTGGTG
TTTTGGGATG GAAAAGAGGA GTCCAGGAAC TACACGGTGG TGAACACAGA GAAGGGCCTC
ATGGAGGTGC AGAGGATGTG CTGCCCTGGG ATGAAGGTGA GCTGCCAGCT CCAGGTCCAG
AGATCCCTGT GGACAGCCAC CGAGGACCAG AAAATCTACA TCTACACCCT CAAGGGCATG
TGCCCCTTAA ACACACCCCA ACAGGCCTTG GATACTCCAG CTGTCGTCAC CTGCTTCTTG
GCCGTGCCTG TTATTAAAAA GAATTCCTAC CTGGTCTTAG CGGGCCTCGC CGATGGGCTT
GTGGCTGTGT TTCCCGTGGT GCGGGGCACC CCAAAGGACA GCTGCTCCTA CCTGTGCTCA
CACACAGCCA ACAGGTCCAA GTTCAGCATC GCGGATGAAG ACGCACGGCA GAACCCCTAC
CCAGTGAAGG CCATGGAGGT GGTCAACAGC GGCTCTGAGG TCTGGTACAG CAATGGGCCG
GGCCTCCTTG TCATCGACTG TGCCTCCCTG GAGATCTGCA GGCGGCTGGA GCCCTACATG
GCCCCCTCCA TGGTTACGTC AGTCGTGTGC AGCTCTGAGG GCAGAGGGGA GGAGGTCGTC
TGGTGCCTGG ATGACAAGGC CAACTCCTTG GTGATGTACC ACTCCACCAC CTACCAGCTG
TGTGCCCGGT ACTTCTGCGG GGTCCCCAGC CCCCTCAGGG ACATGTTTCC CGTGCGGCCC
TTGGACACGG AACCCCCGGC AGCCAGCCAC ACGGCCAACC CAAAGGTGCC TGAGGGGGAC
TCCATCGCGG ACGTGAGCAT CATGTACAGT GAGGAGCTGG GCACGCAGAT CCTGATCCAC
CAGGAATCAC TCACTGACTA CTGCTCCATG TCCTCCTACT CCTCATCCCC ACCCCGCCAG
GCTGCCAGGT CCCCCTCAAG CCTCCCCAGC TCCCCAGCAA GTTCTTCCAG TGTGCCTTTC
TCCACCGACT GCGAGGACTC AGACATGCTA CATACGCCCG GTGCTGCCTC CGACAGGTCT
GAGCATGACC TGACCCCCAT GGACGGGGAG ACCTTCAGCC AGCACCTGCA GGCCGTGAAG
ATCCTCGCCG TCAGAGACCT CATTTGGGTC CCCAGGCGCG GTGGAGATGT TATCGTCATT
GGCCTGGAGA AGGATTCTGG CGCCCAGCGG GGCCGAGTCA TTGCCGTCTT AAAAGCCCGA
GAGCTGACTC CGCATGGGGT GCTGGTGGAT GCTGCCGTGG TGGCAAAGGA CACTGTTGTG
TGCACCTTTG AAAATGAAAA CACAGAGTGG TGCCTGGCCG TCTGGAGGGG CTGGGGCGCC
AGGGAGTTCG ACATTTTCTA CCAGTCCTAC GAGGAGCTGG GCCGGCTGGA GGCTTGCACT
CGCAAGAGAA GGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016878059.1
CDS80..4654
Translation

Target ORF information:

RefSeq Version XM_017022570.1
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017022570.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
ATGTGGATTT CTCAGAAAAC GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATA 
AATGAAGATG GACTGAAAAC GAAGCGTATT GCCTTTTTCA CCACCAGAGG TCGCCAGCGC
TCCGGGACTG AGGCAGCAAG TGTGCTGGAA TTTCCGGCCT TCCTAAGTGA GTCTTTGGAA
GTCCTTTGCC TGAACGACAA CCACCTCGAC ACAGTCCCTC CCTCGGTTTG CCTACTGAAG
AGCTTATCAG AGCTCTACTT GGGAAACAAC CCTGGCCTCC GGGAGCTCCC TCCTGAGCTG
GGGCAGCTGG GCAACCTCTG GCAGCTGGAC ACTGAAGACC TGACCATCAG CAATGTGCCT
GCAGAAATCC AAAAAGAAGG CCCCAAAGCA ATGCTGTCTT ACCTGCGTGC TCAGCTGCGG
AAAGCGGAAA AGTGCAAGCT GATGAAGATG ATCATCGTGG GTCCCCCGCG CCAGGGCAAG
TCCACCCTCC TGGAGATCTT ACAGACGGGG AGGGCCCCCC AGGTGGTGCA TGGAGAGGCC
ACCATCAGGA CCACCAAGTG GGAGCTCCAG AGGCCGGCTG GCTCGAGAGC CAAGGTTGAG
TCCGTGGAGT TCAACGTCTG GGACATCGGG GGACCGGCCA GCATGGCCAC TGTCAACCAG
TGCTTCTTCA CGGACAAGGC CCTGTACGTG GTGGTCTGGA ACCTGGCGCT GGGGGAGGAG
GCCGTGGCCA ACCTCCAGTT CTGGCTGCTC AACATCGAGG CCAAGGCCCC AAACGCCGTG
GTGCTGGTGG TCGGGACGCA CCTGGATTTA ATTGAAGCCA AGTTCCGTGT GGAAAGGATT
GCAACGCTGC GTGCCTATGT GCTGGCACTC TGCCGCTCCC CCTCCGGCTC CAGGGCCACA
GGCTTCCCAG ACATCACCTT CAAACACTTA CATGAGATTT CCTGCAAGAG CCTGGAAGGT
CAGGAAGGGC TGCGACAGCT GATTTTCCAC GTCACGTGCA GCATGAAGGA CGTGGGCAGC
ACCATCGGCT GCCAGCGACT GGCAGGGCGG CTGATCCCCA GGAGCTACCT GAGCCTGCAG
GAGGCCGTGC TGGCAGAGCA GCAGCGCCGC AGCCGGGACG ACGACGTGCA GTACCTGACG
GACAGGCAGC TGGAGCAGCT GGTGGAGCAG ACGCCCGACA ACGACATCAA GGACTACGAG
GACCTGCAGT CAGCCATCAG CTTCCTCATA GAAACCGGCA CCCTGCTCCA TTTCCCGGAC
ACCAGCCACG GCCTGAGGAA CCTCTACTTC CTCGACCCTA TTTGGCTCTC CGAATGTCTG
CAGAGGATCT TTAATATTAA GGGCTCTCGG TCAGTGGCCA AGAATGGGGT GATCAGAGCA
GAAGACCTCA GGATGCTGCT GGTGGGGACT GGCTTCACGC AGCAGACGGA AGAGCAGTAC
TTCCAGTTCC TGGCCAAGTT TGAGATCGCC CTGCCCGTCG CCAATGACAG CTACCTCCTG
CCCCATCTCC TTCCATCTAA ACCTGGCCTG GACACCCACG GTATGCGGCA CCCCACAGCC
AACACCATTC AGAGGGTATT TAAGATGAGC TTCGTTCCCG TTGGCTTCTG GCAAAGGTTT
ATAGCACGGA TGCTGATCAG CCTGGCGGAG ATGGACCTGC AGCTTTTTGA AAACAAGAAG
AATACTAAAA GCAGGAACAG GAAAGTCACC ATTTACAGTT TTACAGGAAA CCAGAGAAAT
CGCTGTAGCA CATTCAGAGT GAAAAGAAAT CAGACCATCT ATTGGCAGGA AGGGCTCCTG
GTCACTTTTG ATGGGGGCTA CCTCAGTGTG GAATCTTCCG ACGTGAACTG GAAAAAGAAG
AAAAGCGGAG GAATGAAAAT TGTTTGCCAA TCAGAAGTGA GGGACTTCTC AGCCATGGCT
TTCATCACGG ACCACGTCAA TTCCTTGATT GATCAGTGGT TTCCCGCCCT GACAGCCACA
GAGAGCGACG GGACGCCACT CATGGAGCAG TACGTGCCCT GCCCGGTCTG CGAGACAGCC
TGGGCCCAGC ACACGGACCC CAGTGAGAAA TCAGAGGATG TGCAGTACTT CGACATGGAA
GACTGTGTCC TGACGGCCAT CGAGCGGGAC TTCATCTCCT GCCCCAGACA CCCGGACCTC
CCCGTGCCGC TGCAGGAGCT GGTCCCTGAA CTGTTCATGA CCGACTTCCC GGCCAGGCTC
TTCCTGGAGA ACAGCAAGCT GGAGCACAGC GAGGACGAGG GCAGCGTCCT GGGCCAGGGC
GGCAGTGGCA CCGTCATCTA CCGGGCCCGG TACCAGGGCC AGCCTGTGGC CGTCAAGCGC
TTCCACATCA AAAAATTCAA GAACTTTGCT AACGTACCGG CAGACACCAT GCTGAGGCAC
CTGCGGGCCA CCGATGCCAT GAAGAACTTC TCCGAGTTCC GGCAGGAGGC CAGCATGCTG
CACGCGCTGC AGCACCCCTG CATCGTGGCG CTCATCGGCA TCAGCATCCA CCCGCTCTGC
TTCGCCCTGG AGCTCGCGCC GCTCAGCAGC CTCAACACCG TGCTGTCCGA GAACGCCAGA
GATTCTTCCT TTATACCCCT GGGACACATG CTCACCCAAA AAATAGCCTA CCAGATCGCC
TCGGGCCTGG CCTACCTGCA CAAGAAAAAC ATCATCTTCT GTGACCTGAA GTCGGACAAC
ATTCTGGTGT GGTCCCTTGA CGTCAAGGAG CACATCAACA TCAAGCTATC TGACTACGGG
ATTTCGAGGC AGTCATTCCA TGAGGGCGCC CTAGGCGTGG AGGGCACTCC TGGCTACCAG
GCCCCAGAGA TCAGGCCTCG CATTGTATAT GATGAGAAGG TAGATATGTT CTCCTATGGA
ATGGTGCTCT ACGAGTTGCT GTCAGGACAG CGCCCTGCAC TGGGCCACCA CCAGCTCCAG
ATTGCCAAGA AGCTGTCCAA GGGCATCCGC CCGGTTCTGG GGCAGCCGGA GGAAGTGCAG
TTCCGGCGAC TGCAGGCGCT CATGATGGAG TGCTGGGACA CTAAGCCAGA GAAGCGACCG
CTGGCCCTGT CGGTGGTGAG CCAGATGAAG GACCCGACTT TTGCCACCTT CATGTATGAA
CTGTGCTGTG GGAAGCAGAC AGCCTTCTTC TCATCCCAGG GCCAGGAGTA CACCGTGGTG
TTTTGGGATG GAAAAGAGGA GTCCAGGAAC TACACGGTGG TGAACACAGA GAAGGGCCTC
ATGGAGGTGC AGAGGATGTG CTGCCCTGGG ATGAAGGTGA GCTGCCAGCT CCAGGTCCAG
AGATCCCTGT GGACAGCCAC CGAGGACCAG AAAATCTACA TCTACACCCT CAAGGGCATG
TGCCCCTTAA ACACACCCCA ACAGGCCTTG GATACTCCAG CTGTCGTCAC CTGCTTCTTG
GCCGTGCCTG TTATTAAAAA GAATTCCTAC CTGGTCTTAG CGGGCCTCGC CGATGGGCTT
GTGGCTGTGT TTCCCGTGGT GCGGGGCACC CCAAAGGACA GCTGCTCCTA CCTGTGCTCA
CACACAGCCA ACAGGTCCAA GTTCAGCATC GCGGATGAAG ACGCACGGCA GAACCCCTAC
CCAGTGAAGG CCATGGAGGT GGTCAACAGC GGCTCTGAGG TCTGGTACAG CAATGGGCCG
GGCCTCCTTG TCATCGACTG TGCCTCCCTG GAGATCTGCA GGCGGCTGGA GCCCTACATG
GCCCCCTCCA TGGTTACGTC AGTCGTGTGC AGCTCTGAGG GCAGAGGGGA GGAGGTCGTC
TGGTGCCTGG ATGACAAGGC CAACTCCTTG GTGATGTACC ACTCCACCAC CTACCAGCTG
TGTGCCCGGT ACTTCTGCGG GGTCCCCAGC CCCCTCAGGG ACATGTTTCC CGTGCGGCCC
TTGGACACGG AACCCCCGGC AGCCAGCCAC ACGGCCAACC CAAAGGTGCC TGAGGGGGAC
TCCATCGCGG ACGTGAGCAT CATGTACAGT GAGGAGCTGG GCACGCAGAT CCTGATCCAC
CAGGAATCAC TCACTGACTA CTGCTCCATG TCCTCCTACT CCTCATCCCC ACCCCGCCAG
GCTGCCAGGT CCCCCTCAAG CCTCCCCAGC TCCCCAGCAA GTTCTTCCAG TGTGCCTTTC
TCCACCGACT GCGAGGACTC AGACATGCTA CATACGCCCG GTGCTGCCTC CGACAGGTCT
GAGCATGACC TGACCCCCAT GGACGGGGAG ACCTTCAGCC AGCACCTGCA GGCCGTGAAG
ATCCTCGCCG TCAGAGACCT CATTTGGGTC CCCAGGCGCG GTGGAGATGT TATCGTCATT
GGCCTGGAGA AGGATTCTGG CGCCCAGCGG GGCCGAGTCA TTGCCGTCTT AAAAGCCCGA
GAGCTGACTC CGCATGGGGT GCTGGTGGAT GCTGCCGTGG TGGCAAAGGA CACTGTTGTG
TGCACCTTTG AAAATGAAAA CACAGAGTGG TGCCTGGCCG TCTGGAGGGG CTGGGGCGCC
AGGGAGTTCG ACATTTTCTA CCAGTCCTAC GAGGAGCTGG GCCGGCTGGA GGCTTGCACT
CGCAAGAGAA GGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72032
Clone ID Related Accession (Same CDS sequence) XM_011522014.2
Accession Version XM_011522014.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5946bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGG TGCTGGTGGA TGCTGCCGTG GTGGCAAAGG ACACTGTTGT GTGCACCTTT
GAAAATGAAA ACACAGAGTG GTGCCTGGCC GTCTGGAGGG GCTGGGGCGC CAGGGAGTTC
GACATTTTCT ACCAGTCCTA CGAGGAGCTG GGCCGGCTGG AGGCTTGCAC TCGCAAGAGA
AGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520316.1
CDS558..6503
Misc Feature(1)840..1199
Misc Feature(2)933..1220
Misc Feature(3)order(933..935,942..950,954..959,969..971,978..980,1017..1019,1023..1025,1029..1034,1041..1049,1053..1058,1068..1070,1080..1082,1113..1115,1119..1121,1140..1142,1152..1157,1164..1172,1176..1181,1191..1193,1200..1202)
Misc Feature(4)1023..1115
Misc Feature(5)1119..1211
Misc Feature(6)<1197..2021
Misc Feature(7)1398..1466
Misc Feature(8)1461..2399
Misc Feature(9)1467..1547
Misc Feature(10)1548..1616
Misc Feature(11)1617..1700
Misc Feature(12)1692..2480
Misc Feature(13)1701..1772
Misc Feature(14)order(1719..1724,1857..1859,2073..2075,2145..2147,2295..2297,2349..2351,2379..2381)
Misc Feature(15)1773..1910
Misc Feature(16)1911..1979
Misc Feature(17)1980..2051
Misc Feature(18)2052..2204
Misc Feature(19)2205..2273
Misc Feature(20)2460..>2900
Misc Feature(21)2472..3020
Misc Feature(22)2490..2513
Misc Feature(23)order(2496..2516,2661..2663,2826..2831,2835..2837,2970..2978)
Misc Feature(24)2556..2558
Misc Feature(25)2577..2585
Misc Feature(26)2652..2663
Misc Feature(27)order(2658..2663,2709..2714)
Misc Feature(28)2826..2837
Misc Feature(29)2970..2978
Misc Feature(30)3087..3665
Misc Feature(31)4290..5090
Misc Feature(32)4299..5126
Misc Feature(33)order(4299..4313,4323..4325,4359..4361,4365..4367,4515..4517,4557..4568,4578..4580,4584..4586,4713..4715,4719..4721,4725..4730,4734..4736,4782..4784,4791..4793,4833..4844)
Misc Feature(34)order(4299..4313,4323..4325,4359..4361,4365..4367,4515..4517,4557..4568,4578..4580,4713..4715,4719..4721,4725..4730,4734..4736,4782..4784)
Misc Feature(35)order(4311..4313,4578..4580,4584..4586,4713..4715,4719..4721,4725..4727,4791..4793,4833..4844)
Misc Feature(36)4779..4844
Translation

Target ORF information:

RefSeq Version XM_011522014.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522014.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGG TGCTGGTGGA TGCTGCCGTG GTGGCAAAGG ACACTGTTGT GTGCACCTTT
GAAAATGAAA ACACAGAGTG GTGCCTGGCC GTCTGGAGGG GCTGGGGCGC CAGGGAGTTC
GACATTTTCT ACCAGTCCTA CGAGGAGCTG GGCCGGCTGG AGGCTTGCAC TCGCAAGAGA
AGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72033
Clone ID Related Accession (Same CDS sequence) XM_011522015.2
Accession Version XM_011522015.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5304bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
ATGACTCAGG AGCCAGAGGC TCTCCGTGTG AAATGGTCCC ATCTCAGACT GCCCTGGGTA 
GACCTAGACT GGCTCATAGA CATCTCCTGC CAGATCACGG AGCTCGACCT TTCTGCCAAC
TGCCTGGCGA CCCTCCCCTC GGTTATCCCC TGGGGCCTCA TCAATCTCCG GAAGCTGAAC
CTCTCCGACA ACCACCTGGG GGAGCTGCCT GGCGTGCAGT CATCGGACGA AATCATCTGT
TCCAGGCTAC TTGAAATTGA CATTTCCAGC AACAAGTTGT CCCACCTCCC TCCTGGATTC
TTGCACCTCT CAAAACTTCA AAAACTGACA GCTTCAAAAA ATTGTTTAGA AAAATTGTTC
GAAGAAGAAA ATGCCACTAA CTGGATAGGT TTACGGAAGC TACAGGAACT TGATATATCT
GACAATAAAT TGACAGAACT CCCTGCCCTG TTCCTTCACT CTTTCAAGTC CCTCAATTCT
CTGAATGTCT CCAGAAACAA CCTGAAGGTG TTTCCAGATC CCTGGGCCTG CCCTTTGAAA
TGTTGTAAAG CTTCCAGAAA TGCCCTGGAA TGTCTGCCAG ACAAAATGGC TGTCTTTTGG
AAAAATCACC TGAAGGATGT GGATTTCTCA GAAAACGCAC TCAAAGAAGT TCCCCTGGGA
CTTTTCCAGC TTGATGCCCT CATGTTCTTG AGGTTACAGG GGAACCAGCT GGCGGCACTT
CCACCTCAAG AGAAGTGGAC CTGCAGGCAG CTCAAAACCC TGGATCTCTC CAGAAACCAA
CTTGGCAAAA ATGAAGATGG ACTGAAAACG AAGCGTATTG CCTTTTTCAC CACCAGAGGT
CGCCAGCGCT CCGGGACTGA GGCAGCAAGT GTGCTGGAAT TTCCGGCCTT CCTAAGTGAG
TCTTTGGAAG TCCTTTGCCT GAACGACAAC CACCTCGACA CAGTCCCTCC CTCGGTTTGC
CTACTGAAGA GCTTATCAGA GCTCTACTTG GGAAACAACC CTGGCCTCCG GGAGCTCCCT
CCTGAGCTGG GGCAGCTGGG CAACCTCTGG CAGCTGGACA CTGAAGACCT GACCATCAGC
AATGTGCCTG CAGAAATCCA AAAAGAAGGC CCCAAAGCAA TGCTGTCTTA CCTGCGTGCT
CAGCTGCGGA AAGCGGAAAA GTGCAAGCTG ATGAAGATGA TCATCGTGGG TCCCCCGCGC
CAGGGCAAGT CCACCCTCCT GGAGATCTTA CAGACGGGGA GGGCCCCCCA GGTGGTGCAT
GGAGAGGCCA CCATCAGGAC CACCAAGTGG GAGCTCCAGA GGCCGGCTGG CTCGAGAGCC
AAGGTTGAGT CCGTGGAGTT CAACGTCTGG GACATCGGGG GACCGGCCAG CATGGCCACT
GTCAACCAGT GCTTCTTCAC GGACAAGGCC CTGTACGTGG TGGTCTGGAA CCTGGCGCTG
GGGGAGGAGG CCGTGGCCAA CCTCCAGTTC TGGCTGCTCA ACATCGAGGC CAAGGCCCCA
AACGCCGTGG TGCTGGTGGT CGGGACGCAC CTGGATTTAA TTGAAGCCAA GTTCCGTGTG
GAAAGGATTG CAACGCTGCG TGCCTATGTG CTGGCACTCT GCCGCTCCCC CTCCGGCTCC
AGGGCCACAG GCTTCCCAGA CATCACCTTC AAACACTTAC ATGAGATTTC CTGCAAGAGC
CTGGAAGGTC AGGAAGGGCT GCGACAGCTG ATTTTCCACG TCACGTGCAG CATGAAGGAC
GTGGGCAGCA CCATCGGCTG CCAGCGACTG GCAGGGCGGC TGATCCCCAG GAGCTACCTG
AGCCTGCAGG AGGCCGTGCT GGCAGAGCAG CAGCGCCGCA GCCGGGACGA CGACGTGCAG
TACCTGACGG ACAGGCAGCT GGAGCAGCTG GTGGAGCAGA CGCCCGACAA CGACATCAAG
GACTACGAGG ACCTGCAGTC AGCCATCAGC TTCCTCATAG AAACCGGCAC CCTGCTCCAT
TTCCCGGACA CCAGCCACGG CCTGAGGAAC CTCTACTTCC TCGACCCTAT TTGGCTCTCC
GAATGTCTGC AGAGGATCTT TAATATTAAG GGCTCTCGGT CAGTGGCCAA GAATGGGGTG
ATCAGAGCAG AAGACCTCAG GATGCTGCTG GTGGGGACTG GCTTCACGCA GCAGACGGAA
GAGCAGTACT TCCAGTTCCT GGCCAAGTTT GAGATCGCCC TGCCCGTCGC CAATGACAGC
TACCTCCTGC CCCATCTCCT TCCATCTAAA CCTGGCCTGG ACACCCACGG TATGCGGCAC
CCCACAGCCA ACACCATTCA GAGGGTATTT AAGATGAGCT TCGTTCCCGT TGGCTTCTGG
CAAAGGTTTA TAGCACGGAT GCTGATCAGC CTGGCGGAGA TGGACCTGCA GCTTTTTGAA
AACAAGAAGA ATACTAAAAG CAGGAACAGG AAAGTCACCA TTTACAGTTT TACAGGAAAC
CAGAGAAATC GCTGTAGCAC ATTCAGAGTG AAAAGAAATC AGACCATCTA TTGGCAGGAA
GGGCTCCTGG TCACTTTTGA TGGGGGCTAC CTCAGTGTGG AATCTTCCGA CGTGAACTGG
AAAAAGAAGA AAAGCGGAGG AATGAAAATT GTTTGCCAAT CAGAAGTGAG GGACTTCTCA
GCCATGGCTT TCATCACGGA CCACGTCAAT TCCTTGATTG ATCAGTGGTT TCCCGCCCTG
ACAGCCACAG AGAGCGACGG GACGCCACTC ATGGAGCAGT ACGTGCCCTG CCCGGTCTGC
GAGACAGCCT GGGCCCAGCA CACGGACCCC AGTGAGAAAT CAGAGGATGT GCAGTACTTC
GACATGGAAG ACTGTGTCCT GACGGCCATC GAGCGGGACT TCATCTCCTG CCCCAGACAC
CCGGACCTCC CCGTGCCGCT GCAGGAGCTG GTCCCTGAAC TGTTCATGAC CGACTTCCCG
GCCAGGCTCT TCCTGGAGAA CAGCAAGCTG GAGCACAGCG AGGACGAGGG CAGCGTCCTG
GGCCAGGGCG GCAGTGGCAC CGTCATCTAC CGGGCCCGGT ACCAGGGCCA GCCTGTGGCC
GTCAAGCGCT TCCACATCAA AAAATTCAAG AACTTTGCTA ACGTACCGGC AGACACCATG
CTGAGGCACC TGCGGGCCAC CGATGCCATG AAGAACTTCT CCGAGTTCCG GCAGGAGGCC
AGCATGCTGC ACGCGCTGCA GCACCCCTGC ATCGTGGCGC TCATCGGCAT CAGCATCCAC
CCGCTCTGCT TCGCCCTGGA GCTCGCGCCG CTCAGCAGCC TCAACACCGT GCTGTCCGAG
AACGCCAGAG ATTCTTCCTT TATACCCCTG GGACACATGC TCACCCAAAA AATAGCCTAC
CAGATCGCCT CGGGCCTGGC CTACCTGCAC AAGAAAAACA TCATCTTCTG TGACCTGAAG
TCGGACAACA TTCTGGTGTG GTCCCTTGAC GTCAAGGAGC ACATCAACAT CAAGCTATCT
GACTACGGGA TTTCGAGGCA GTCATTCCAT GAGGGCGCCC TAGGCGTGGA GGGCACTCCT
GGCTACCAGG CCCCAGAGAT CAGGCCTCGC ATTGTATATG ATGAGAAGGT AGATATGTTC
TCCTATGGAA TGGTGCTCTA CGAGTTGCTG TCAGGACAGC GCCCTGCACT GGGCCACCAC
CAGCTCCAGA TTGCCAAGAA GCTGTCCAAG GGCATCCGCC CGGTTCTGGG GCAGCCGGAG
GAAGTGCAGT TCCGGCGACT GCAGGCGCTC ATGATGGAGT GCTGGGACAC TAAGCCAGAG
AAGCGACCGC TGGCCCTGTC GGTGGTGAGC CAGATGAAGG ACCCGACTTT TGCCACCTTC
ATGTATGAAC TGTGCTGTGG GAAGCAGACA GCCTTCTTCT CATCCCAGGG CCAGGAGTAC
ACCGTGGTGT TTTGGGATGG AAAAGAGGAG TCCAGGAACT ACACGGTGGT GAACACAGAG
AAGGGCCTCA TGGAGGTGCA GAGGATGTGC TGCCCTGGGA TGAAGGTGAG CTGCCAGCTC
CAGGTCCAGA GATCCCTGTG GACAGCCACC GAGGACCAGA AAATCTACAT CTACACCCTC
AAGGGCATGT GCCCCTTAAA CACACCCCAA CAGGCCTTGG ATACTCCAGC TGTCGTCACC
TGCTTCTTGG CCGTGCCTGT TATTAAAAAG AATTCCTACC TGGTCTTAGC GGGCCTCGCC
GATGGGCTTG TGGCTGTGTT TCCCGTGGTG CGGGGCACCC CAAAGGACAG CTGCTCCTAC
CTGTGCTCAC ACACAGCCAA CAGGTCCAAG TTCAGCATCG CGGATGAAGA CGCACGGCAG
AACCCCTACC CAGTGAAGGC CATGGAGGTG GTCAACAGCG GCTCTGAGGT CTGGTACAGC
AATGGGCCGG GCCTCCTTGT CATCGACTGT GCCTCCCTGG AGATCTGCAG GCGGCTGGAG
CCCTACATGG CCCCCTCCAT GGTTACGTCA GTCGTGTGCA GCTCTGAGGG CAGAGGGGAG
GAGGTCGTCT GGTGCCTGGA TGACAAGGCC AACTCCTTGG TGATGTACCA CTCCACCACC
TACCAGCTGT GTGCCCGGTA CTTCTGCGGG GTCCCCAGCC CCCTCAGGGA CATGTTTCCC
GTGCGGCCCT TGGACACGGA ACCCCCGGCA GCCAGCCACA CGGCCAACCC AAAGGTGCCT
GAGGGGGACT CCATCGCGGA CGTGAGCATC ATGTACAGTG AGGAGCTGGG CACGCAGATC
CTGATCCACC AGGAATCACT CACTGACTAC TGCTCCATGT CCTCCTACTC CTCATCCCCA
CCCCGCCAGG CTGCCAGGTC CCCCTCAAGC CTCCCCAGCT CCCCAGCAAG TTCTTCCAGT
GTGCCTTTCT CCACCGACTG CGAGGACTCA GACATGCTAC ATACGCCCGG TGCTGCCTCC
GACAGGTCTG AGCATGACCT GACCCCCATG GACGGGGAGA CCTTCAGCCA GCACCTGCAG
GCCGTGAAGA TCCTCGCCGT CAGAGACCTC ATTTGGGTCC CCAGGCGCGG TGGAGATGTT
ATCGTCATTG GCCTGGAGAA GGATTCTGGC GCCCAGCGGG GCCGAGTCAT TGCCGTCTTA
AAAGCCCGAG AGCTGACTCC GCATGGGGTG CTGGTGGATG CTGCCGTGGT GGCAAAGGAC
ACTGTTGTGT GCACCTTTGA AAATGAAAAC ACAGAGTGGT GCCTGGCCGT CTGGAGGGGC
TGGGGCGCCA GGGAGTTCGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGCTGGAG
GCTTGCACTC GCAAGAGAAG GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520317.1
CDS1897..7200
Misc Feature(1)1993..2061
Misc Feature(2)2056..2994
Misc Feature(3)<2059..2616
Misc Feature(4)2062..2142
Misc Feature(5)2143..2211
Misc Feature(6)2212..2295
Misc Feature(7)2296..2367
Misc Feature(8)2368..2505
Misc Feature(9)2506..2574
Misc Feature(10)2575..2646
Misc Feature(11)2647..2799
Misc Feature(12)2800..2868
Misc Feature(13)3055..>3495
Misc Feature(14)3067..3615
Misc Feature(15)3085..3108
Misc Feature(16)order(3091..3111,3256..3258,3421..3426,3430..3432,3565..3573)
Misc Feature(17)3151..3153
Misc Feature(18)3172..3180
Misc Feature(19)3247..3258
Misc Feature(20)order(3253..3258,3304..3309)
Misc Feature(21)3421..3432
Misc Feature(22)3565..3573
Misc Feature(23)3682..4260
Misc Feature(24)4885..5685
Misc Feature(25)4894..5721
Misc Feature(26)order(4894..4908,4918..4920,4954..4956,4960..4962,5110..5112,5152..5163,5173..5175,5179..5181,5308..5310,5314..5316,5320..5325,5329..5331,5377..5379,5386..5388,5428..5439)
Misc Feature(27)order(4894..4908,4918..4920,4954..4956,4960..4962,5110..5112,5152..5163,5173..5175,5308..5310,5314..5316,5320..5325,5329..5331,5377..5379)
Misc Feature(28)order(4906..4908,5173..5175,5179..5181,5308..5310,5314..5316,5320..5322,5386..5388,5428..5439)
Misc Feature(29)5374..5439
Translation

Target ORF information:

RefSeq Version XM_011522015.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522015.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
ATGACTCAGG AGCCAGAGGC TCTCCGTGTG AAATGGTCCC ATCTCAGACT GCCCTGGGTA 
GACCTAGACT GGCTCATAGA CATCTCCTGC CAGATCACGG AGCTCGACCT TTCTGCCAAC
TGCCTGGCGA CCCTCCCCTC GGTTATCCCC TGGGGCCTCA TCAATCTCCG GAAGCTGAAC
CTCTCCGACA ACCACCTGGG GGAGCTGCCT GGCGTGCAGT CATCGGACGA AATCATCTGT
TCCAGGCTAC TTGAAATTGA CATTTCCAGC AACAAGTTGT CCCACCTCCC TCCTGGATTC
TTGCACCTCT CAAAACTTCA AAAACTGACA GCTTCAAAAA ATTGTTTAGA AAAATTGTTC
GAAGAAGAAA ATGCCACTAA CTGGATAGGT TTACGGAAGC TACAGGAACT TGATATATCT
GACAATAAAT TGACAGAACT CCCTGCCCTG TTCCTTCACT CTTTCAAGTC CCTCAATTCT
CTGAATGTCT CCAGAAACAA CCTGAAGGTG TTTCCAGATC CCTGGGCCTG CCCTTTGAAA
TGTTGTAAAG CTTCCAGAAA TGCCCTGGAA TGTCTGCCAG ACAAAATGGC TGTCTTTTGG
AAAAATCACC TGAAGGATGT GGATTTCTCA GAAAACGCAC TCAAAGAAGT TCCCCTGGGA
CTTTTCCAGC TTGATGCCCT CATGTTCTTG AGGTTACAGG GGAACCAGCT GGCGGCACTT
CCACCTCAAG AGAAGTGGAC CTGCAGGCAG CTCAAAACCC TGGATCTCTC CAGAAACCAA
CTTGGCAAAA ATGAAGATGG ACTGAAAACG AAGCGTATTG CCTTTTTCAC CACCAGAGGT
CGCCAGCGCT CCGGGACTGA GGCAGCAAGT GTGCTGGAAT TTCCGGCCTT CCTAAGTGAG
TCTTTGGAAG TCCTTTGCCT GAACGACAAC CACCTCGACA CAGTCCCTCC CTCGGTTTGC
CTACTGAAGA GCTTATCAGA GCTCTACTTG GGAAACAACC CTGGCCTCCG GGAGCTCCCT
CCTGAGCTGG GGCAGCTGGG CAACCTCTGG CAGCTGGACA CTGAAGACCT GACCATCAGC
AATGTGCCTG CAGAAATCCA AAAAGAAGGC CCCAAAGCAA TGCTGTCTTA CCTGCGTGCT
CAGCTGCGGA AAGCGGAAAA GTGCAAGCTG ATGAAGATGA TCATCGTGGG TCCCCCGCGC
CAGGGCAAGT CCACCCTCCT GGAGATCTTA CAGACGGGGA GGGCCCCCCA GGTGGTGCAT
GGAGAGGCCA CCATCAGGAC CACCAAGTGG GAGCTCCAGA GGCCGGCTGG CTCGAGAGCC
AAGGTTGAGT CCGTGGAGTT CAACGTCTGG GACATCGGGG GACCGGCCAG CATGGCCACT
GTCAACCAGT GCTTCTTCAC GGACAAGGCC CTGTACGTGG TGGTCTGGAA CCTGGCGCTG
GGGGAGGAGG CCGTGGCCAA CCTCCAGTTC TGGCTGCTCA ACATCGAGGC CAAGGCCCCA
AACGCCGTGG TGCTGGTGGT CGGGACGCAC CTGGATTTAA TTGAAGCCAA GTTCCGTGTG
GAAAGGATTG CAACGCTGCG TGCCTATGTG CTGGCACTCT GCCGCTCCCC CTCCGGCTCC
AGGGCCACAG GCTTCCCAGA CATCACCTTC AAACACTTAC ATGAGATTTC CTGCAAGAGC
CTGGAAGGTC AGGAAGGGCT GCGACAGCTG ATTTTCCACG TCACGTGCAG CATGAAGGAC
GTGGGCAGCA CCATCGGCTG CCAGCGACTG GCAGGGCGGC TGATCCCCAG GAGCTACCTG
AGCCTGCAGG AGGCCGTGCT GGCAGAGCAG CAGCGCCGCA GCCGGGACGA CGACGTGCAG
TACCTGACGG ACAGGCAGCT GGAGCAGCTG GTGGAGCAGA CGCCCGACAA CGACATCAAG
GACTACGAGG ACCTGCAGTC AGCCATCAGC TTCCTCATAG AAACCGGCAC CCTGCTCCAT
TTCCCGGACA CCAGCCACGG CCTGAGGAAC CTCTACTTCC TCGACCCTAT TTGGCTCTCC
GAATGTCTGC AGAGGATCTT TAATATTAAG GGCTCTCGGT CAGTGGCCAA GAATGGGGTG
ATCAGAGCAG AAGACCTCAG GATGCTGCTG GTGGGGACTG GCTTCACGCA GCAGACGGAA
GAGCAGTACT TCCAGTTCCT GGCCAAGTTT GAGATCGCCC TGCCCGTCGC CAATGACAGC
TACCTCCTGC CCCATCTCCT TCCATCTAAA CCTGGCCTGG ACACCCACGG TATGCGGCAC
CCCACAGCCA ACACCATTCA GAGGGTATTT AAGATGAGCT TCGTTCCCGT TGGCTTCTGG
CAAAGGTTTA TAGCACGGAT GCTGATCAGC CTGGCGGAGA TGGACCTGCA GCTTTTTGAA
AACAAGAAGA ATACTAAAAG CAGGAACAGG AAAGTCACCA TTTACAGTTT TACAGGAAAC
CAGAGAAATC GCTGTAGCAC ATTCAGAGTG AAAAGAAATC AGACCATCTA TTGGCAGGAA
GGGCTCCTGG TCACTTTTGA TGGGGGCTAC CTCAGTGTGG AATCTTCCGA CGTGAACTGG
AAAAAGAAGA AAAGCGGAGG AATGAAAATT GTTTGCCAAT CAGAAGTGAG GGACTTCTCA
GCCATGGCTT TCATCACGGA CCACGTCAAT TCCTTGATTG ATCAGTGGTT TCCCGCCCTG
ACAGCCACAG AGAGCGACGG GACGCCACTC ATGGAGCAGT ACGTGCCCTG CCCGGTCTGC
GAGACAGCCT GGGCCCAGCA CACGGACCCC AGTGAGAAAT CAGAGGATGT GCAGTACTTC
GACATGGAAG ACTGTGTCCT GACGGCCATC GAGCGGGACT TCATCTCCTG CCCCAGACAC
CCGGACCTCC CCGTGCCGCT GCAGGAGCTG GTCCCTGAAC TGTTCATGAC CGACTTCCCG
GCCAGGCTCT TCCTGGAGAA CAGCAAGCTG GAGCACAGCG AGGACGAGGG CAGCGTCCTG
GGCCAGGGCG GCAGTGGCAC CGTCATCTAC CGGGCCCGGT ACCAGGGCCA GCCTGTGGCC
GTCAAGCGCT TCCACATCAA AAAATTCAAG AACTTTGCTA ACGTACCGGC AGACACCATG
CTGAGGCACC TGCGGGCCAC CGATGCCATG AAGAACTTCT CCGAGTTCCG GCAGGAGGCC
AGCATGCTGC ACGCGCTGCA GCACCCCTGC ATCGTGGCGC TCATCGGCAT CAGCATCCAC
CCGCTCTGCT TCGCCCTGGA GCTCGCGCCG CTCAGCAGCC TCAACACCGT GCTGTCCGAG
AACGCCAGAG ATTCTTCCTT TATACCCCTG GGACACATGC TCACCCAAAA AATAGCCTAC
CAGATCGCCT CGGGCCTGGC CTACCTGCAC AAGAAAAACA TCATCTTCTG TGACCTGAAG
TCGGACAACA TTCTGGTGTG GTCCCTTGAC GTCAAGGAGC ACATCAACAT CAAGCTATCT
GACTACGGGA TTTCGAGGCA GTCATTCCAT GAGGGCGCCC TAGGCGTGGA GGGCACTCCT
GGCTACCAGG CCCCAGAGAT CAGGCCTCGC ATTGTATATG ATGAGAAGGT AGATATGTTC
TCCTATGGAA TGGTGCTCTA CGAGTTGCTG TCAGGACAGC GCCCTGCACT GGGCCACCAC
CAGCTCCAGA TTGCCAAGAA GCTGTCCAAG GGCATCCGCC CGGTTCTGGG GCAGCCGGAG
GAAGTGCAGT TCCGGCGACT GCAGGCGCTC ATGATGGAGT GCTGGGACAC TAAGCCAGAG
AAGCGACCGC TGGCCCTGTC GGTGGTGAGC CAGATGAAGG ACCCGACTTT TGCCACCTTC
ATGTATGAAC TGTGCTGTGG GAAGCAGACA GCCTTCTTCT CATCCCAGGG CCAGGAGTAC
ACCGTGGTGT TTTGGGATGG AAAAGAGGAG TCCAGGAACT ACACGGTGGT GAACACAGAG
AAGGGCCTCA TGGAGGTGCA GAGGATGTGC TGCCCTGGGA TGAAGGTGAG CTGCCAGCTC
CAGGTCCAGA GATCCCTGTG GACAGCCACC GAGGACCAGA AAATCTACAT CTACACCCTC
AAGGGCATGT GCCCCTTAAA CACACCCCAA CAGGCCTTGG ATACTCCAGC TGTCGTCACC
TGCTTCTTGG CCGTGCCTGT TATTAAAAAG AATTCCTACC TGGTCTTAGC GGGCCTCGCC
GATGGGCTTG TGGCTGTGTT TCCCGTGGTG CGGGGCACCC CAAAGGACAG CTGCTCCTAC
CTGTGCTCAC ACACAGCCAA CAGGTCCAAG TTCAGCATCG CGGATGAAGA CGCACGGCAG
AACCCCTACC CAGTGAAGGC CATGGAGGTG GTCAACAGCG GCTCTGAGGT CTGGTACAGC
AATGGGCCGG GCCTCCTTGT CATCGACTGT GCCTCCCTGG AGATCTGCAG GCGGCTGGAG
CCCTACATGG CCCCCTCCAT GGTTACGTCA GTCGTGTGCA GCTCTGAGGG CAGAGGGGAG
GAGGTCGTCT GGTGCCTGGA TGACAAGGCC AACTCCTTGG TGATGTACCA CTCCACCACC
TACCAGCTGT GTGCCCGGTA CTTCTGCGGG GTCCCCAGCC CCCTCAGGGA CATGTTTCCC
GTGCGGCCCT TGGACACGGA ACCCCCGGCA GCCAGCCACA CGGCCAACCC AAAGGTGCCT
GAGGGGGACT CCATCGCGGA CGTGAGCATC ATGTACAGTG AGGAGCTGGG CACGCAGATC
CTGATCCACC AGGAATCACT CACTGACTAC TGCTCCATGT CCTCCTACTC CTCATCCCCA
CCCCGCCAGG CTGCCAGGTC CCCCTCAAGC CTCCCCAGCT CCCCAGCAAG TTCTTCCAGT
GTGCCTTTCT CCACCGACTG CGAGGACTCA GACATGCTAC ATACGCCCGG TGCTGCCTCC
GACAGGTCTG AGCATGACCT GACCCCCATG GACGGGGAGA CCTTCAGCCA GCACCTGCAG
GCCGTGAAGA TCCTCGCCGT CAGAGACCTC ATTTGGGTCC CCAGGCGCGG TGGAGATGTT
ATCGTCATTG GCCTGGAGAA GGATTCTGGC GCCCAGCGGG GCCGAGTCAT TGCCGTCTTA
AAAGCCCGAG AGCTGACTCC GCATGGGGTG CTGGTGGATG CTGCCGTGGT GGCAAAGGAC
ACTGTTGTGT GCACCTTTGA AAATGAAAAC ACAGAGTGGT GCCTGGCCGT CTGGAGGGGC
TGGGGCGCCA GGGAGTTCGA CATTTTCTAC CAGTCCTACG AGGAGCTGGG CCGGCTGGAG
GCTTGCACTC GCAAGAGAAG GTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72034
Clone ID Related Accession (Same CDS sequence) XM_011522016.2
Accession Version XM_011522016.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5745bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
ATGGTCCGCT ACCTACTCAG CAAGAGACTG GTGGAGCTGC CCACCGAGCC CACGGATGAC 
AACCCAGCCG TGGTGGCAGC GTATTTTGGA CACACGGCAG TTGTGCAGGA ATTGCTTGAG
TCCTTACCAG GTCCCTGCAG TCCCCAGCGG CTTCTGAACT GGATGCTGGC CTTGGCTTGC
CAGCGAGGGC ACCTGGGGGT TGTGAAGCTC CTGGTCCTGA CGCACGGGGC TGACCCGGAG
AGCTACGCTG TCAGGAAGAA TGAGTTCCCT GTCATCGTGC GCTTGCCCCT GTATGCGGCC
ATCAAGTCAG GGAATGAAGA CATTGCAATA TTCCTGCTTC GGCATGGGGC CTATTTCTGT
TCCTACATCT TGCTGGATAG TCCTGACCCC AGCAAACATC TGCTGAGAAA GTACTTCATT
GAAGCCAGTC CCTTGCCCAG CAGTTATCCG GGAAAAACAG CTCTCCGTGT GAAATGGTCC
CATCTCAGAC TGCCCTGGGT AGACCTAGAC TGGCTCATAG ACATCTCCTG CCAGATCACG
GAGCTCGACC TTTCTGCCAA CTGCCTGGCG ACCCTCCCCT CGGTTATCCC CTGGGGCCTC
ATCAATCTCC GGAAGCTGAA CCTCTCCGAC AACCACCTGG GGGAGCTGCC TGGCGTGCAG
TCATCGGACG AAATCATCTG TTCCAGGCTA CTTGAAATTG ACATTTCCAG CAACAAGTTG
TCCCACCTCC CTCCTGGATT CTTGCACCTC TCAAAACTTC AAAAACTGAC AGCTTCAAAA
AATTGTTTAG AAAAATTGTT CGAAGAAGAA AATGCCACTA ACTGGATAGG TTTACGGAAG
CTACAGGAAC TTGATATATC TGACAATAAA TTGACAGAAC TCCCTGCCCT GTTCCTTCAC
TCTTTCAAGT CCCTCAATTC TCTGAATGTC TCCAGAAACA ACCTGAAGGT GTTTCCAGAT
CCCTGGGCCT GCCCTTTGAA ATGTTGTAAA GCTTCCAGAA ATGCCCTGGA ATGTCTGCCA
GACAAAATGG CTGTCTTTTG GAAAAATCAC CTGAAGGATG TGGATTTCTC AGAAAACGCA
CTCAAAGAAG TTCCCCTGGG ACTTTTCCAG CTTGATGCCC TCATGTTCTT GAGGTTACAG
GGGAACCAGC TGGCGGCACT TCCACCTCAA GAGAAGTGGA CCTGCAGGCA GCTCAAAACC
CTGGATCTCT CCAGAAACCA ACTTGGCAAA AATGAAGATG GACTGAAAAC GAAGCGTATT
GCCTTTTTCA CCACCAGAGG TCGCCAGCGC TCCGGGACTG AGGCAGCAAG TGTGCTGGAA
TTTCCGGCCT TCCTAAGTGA GTCTTTGGAA GTCCTTTGCC TGAACGACAA CCACCTCGAC
ACAGTCCCTC CCTCGGTTTG CCTACTGAAG AGCTTATCAG AGCTCTACTT GGGAAACAAC
CCTGGCCTCC GGGAGCTCCC TCCTGAGCTG GGGCAGCTGG GCAACCTCTG GCAGCTGGAC
ACTGAAGACC TGACCATCAG CAATGTGCCT GCAGAAATCC AAAAAGAAGG CCCCAAAGCA
ATGCTGTCTT ACCTGCGTGC TCAGCTGCGG AAAGCGGAAA AGTGCAAGCT GATGAAGATG
ATCATCGTGG GTCCCCCGCG CCAGGGCAAG TCCACCCTCC TGGAGATCTT ACAGACGGGG
AGGGCCCCCC AGGTGGTGCA TGGAGAGGCC ACCATCAGGA CCACCAAGTG GGAGCTCCAG
AGGCCGGCTG GCTCGAGAGC CAAGGTTGAG TCCGTGGAGT TCAACGTCTG GGACATCGGG
GGACCGGCCA GCATGGCCAC TGTCAACCAG TGCTTCTTCA CGGACAAGGC CCTGTACGTG
GTGGTCTGGA ACCTGGCGCT GGGGGAGGAG GCCGTGGCCA ACCTCCAGTT CTGGCTGCTC
AACATCGAGG CCAAGGCCCC AAACGCCGTG GTGCTGGTGG TCGGGACGCA CCTGGATTTA
ATTGAAGCCA AGTTCCGTGT GGAAAGGATT GCAACGCTGC GTGCCTATGT GCTGGCACTC
TGCCGCTCCC CCTCCGGCTC CAGGGCCACA GGCTTCCCAG ACATCACCTT CAAACACTTA
CATGAGATTT CCTGCAAGAG CCTGGAAGGT CAGGAAGGGC TGCGACAGCT GATTTTCCAC
GTCACGTGCA GCATGAAGGA CGTGGGCAGC ACCATCGGCT GCCAGCGACT GGCAGGGCGG
CTGATCCCCA GGAGCTACCT GAGCCTGCAG GAGGCCGTGC TGGCAGAGCA GCAGCGCCGC
AGCCGGGACG ACGACGTGCA GTACCTGACG GACAGGCAGC TGGAGCAGCT GGTGGAGCAG
ACGCCCGACA ACGACATCAA GGACTACGAG GACCTGCAGT CAGCCATCAG CTTCCTCATA
GAAACCGGCA CCCTGCTCCA TTTCCCGGAC ACCAGCCACG GCCTGAGGAA CCTCTACTTC
CTCGACCCTA TTTGGCTCTC CGAATGTCTG CAGAGGATCT TTAATATTAA GGGCTCTCGG
TCAGTGGCCA AGAATGGGGT GATCAGAGCA GAAGACCTCA GGATGCTGCT GGTGGGGACT
GGCTTCACGC AGCAGACGGA AGAGCAGTAC TTCCAGTTCC TGGCCAAGTT TGAGATCGCC
CTGCCCGTCG CCAATGACAG CTACCTCCTG CCCCATCTCC TTCCATCTAA ACCTGGCCTG
GACACCCACG GTATGCGGCA CCCCACAGCC AACACCATTC AGAGGGTATT TAAGATGAGC
TTCGTTCCCG TTGGCTTCTG GCAAAGGTTT ATAGCACGGA TGCTGATCAG CCTGGCGGAG
ATGGACCTGC AGCTTTTTGA AAACAAGAAG AATACTAAAA GCAGGAACAG GAAAGTCACC
ATTTACAGTT TTACAGGAAA CCAGAGAAAT CGCTGTAGCA CATTCAGAGT GAAAAGAAAT
CAGACCATCT ATTGGCAGGA AGGGCTCCTG GTCACTTTTG ATGGGGGCTA CCTCAGTGTG
GAATCTTCCG ACGTGAACTG GAAAAAGAAG AAAAGCGGAG GAATGAAAAT TGTTTGCCAA
TCAGAAGTGA GGGACTTCTC AGCCATGGCT TTCATCACGG ACCACGTCAA TTCCTTGATT
GATCAGTGGT TTCCCGCCCT GACAGCCACA GAGAGCGACG GGACGCCACT CATGGAGCAG
TACGTGCCCT GCCCGGTCTG CGAGACAGCC TGGGCCCAGC ACACGGACCC CAGTGAGAAA
TCAGAGGATG TGCAGTACTT CGACATGGAA GACTGTGTCC TGACGGCCAT CGAGCGGGAC
TTCATCTCCT GCCCCAGACA CCCGGACCTC CCCGTGCCGC TGCAGGAGCT GGTCCCTGAA
CTGTTCATGA CCGACTTCCC GGCCAGGCTC TTCCTGGAGA ACAGCAAGCT GGAGCACAGC
GAGGACGAGG GCAGCGTCCT GGGCCAGGGC GGCAGTGGCA CCGTCATCTA CCGGGCCCGG
TACCAGGGCC AGCCTGTGGC CGTCAAGCGC TTCCACATCA AAAAATTCAA GAACTTTGCT
AACGTACCGG CAGACACCAT GCTGAGGCAC CTGCGGGCCA CCGATGCCAT GAAGAACTTC
TCCGAGTTCC GGCAGGAGGC CAGCATGCTG CACGCGCTGC AGCACCCCTG CATCGTGGCG
CTCATCGGCA TCAGCATCCA CCCGCTCTGC TTCGCCCTGG AGCTCGCGCC GCTCAGCAGC
CTCAACACCG TGCTGTCCGA GAACGCCAGA GATTCTTCCT TTATACCCCT GGGACACATG
CTCACCCAAA AAATAGCCTA CCAGATCGCC TCGGGCCTGG CCTACCTGCA CAAGAAAAAC
ATCATCTTCT GTGACCTGAA GTCGGACAAC ATTCTGGTGT GGTCCCTTGA CGTCAAGGAG
CACATCAACA TCAAGCTATC TGACTACGGG ATTTCGAGGC AGTCATTCCA TGAGGGCGCC
CTAGGCGTGG AGGGCACTCC TGGCTACCAG GCCCCAGAGA TCAGGCCTCG CATTGTATAT
GATGAGAAGG TAGATATGTT CTCCTATGGA ATGGTGCTCT ACGAGTTGCT GTCAGGACAG
CGCCCTGCAC TGGGCCACCA CCAGCTCCAG ATTGCCAAGA AGCTGTCCAA GGGCATCCGC
CCGGTTCTGG GGCAGCCGGA GGAAGTGCAG TTCCGGCGAC TGCAGGCGCT CATGATGGAG
TGCTGGGACA CTAAGCCAGA GAAGCGACCG CTGGCCCTGT CGGTGGTGAG CCAGATGAAG
GACCCGACTT TTGCCACCTT CATGTATGAA CTGTGCTGTG GGAAGCAGAC AGCCTTCTTC
TCATCCCAGG GCCAGGAGTA CACCGTGGTG TTTTGGGATG GAAAAGAGGA GTCCAGGAAC
TACACGGTGG TGAACACAGA GAAGGGCCTC ATGGAGGTGC AGAGGATGTG CTGCCCTGGG
ATGAAGGTGA GCTGCCAGCT CCAGGTCCAG AGATCCCTGT GGACAGCCAC CGAGGACCAG
AAAATCTACA TCTACACCCT CAAGGGCATG TGCCCCTTAA ACACACCCCA ACAGGCCTTG
GATACTCCAG CTGTCGTCAC CTGCTTCTTG GCCGTGCCTG TTATTAAAAA GAATTCCTAC
CTGGTCTTAG CGGGCCTCGC CGATGGGCTT GTGGCTGTGT TTCCCGTGGT GCGGGGCACC
CCAAAGGACA GCTGCTCCTA CCTGTGCTCA CACACAGCCA ACAGGTCCAA GTTCAGCATC
GCGGATGAAG ACGCACGGCA GAACCCCTAC CCAGTGAAGG CCATGGAGGT GGTCAACAGC
GGCTCTGAGG TCTGGTACAG CAATGGGCCG GGCCTCCTTG TCATCGACTG TGCCTCCCTG
GAGATCTGCA GGCGGCTGGA GCCCTACATG GCCCCCTCCA TGGTTACGTC AGTCGTGTGC
AGCTCTGAGG GCAGAGGGGA GGAGGTCGTC TGGTGCCTGG ATGACAAGGC CAACTCCTTG
GTGATGTACC ACTCCACCAC CTACCAGCTG TGTGCCCGGT ACTTCTGCGG GGTCCCCAGC
CCCCTCAGGG ACATGTTTCC CGTGCGGCCC TTGGACACGG AACCCCCGGC AGCCAGCCAC
ACGGCCAACC CAAAGGTGCC TGAGGGGGAC TCCATCGCGG ACGTGAGCAT CATGTACAGT
GAGGAGCTGG GCACGCAGAT CCTGATCCAC CAGGAATCAC TCACTGACTA CTGCTCCATG
TCCTCCTACT CCTCATCCCC ACCCCGCCAG GCTGCCAGGT CCCCCTCAAG CCTCCCCAGC
TCCCCAGCAA GTTCTTCCAG TGTGCCTTTC TCCACCGACT GCGAGGACTC AGACATGCTA
CATACGCCCG GTGCTGCCTC CGACAGGTCT GAGCATGACC TGACCCCCAT GGACGGGGAG
ACCTTCAGCC AGCACCTGCA GGCCGTGAAG ATCCTCGCCG TCAGAGACCT CATTTGGGTC
CCCAGGCGCG GTGGAGATGT TATCGTCATT GGCCTGGAGA AGGATTCTGG CGCCCAGCGG
GGCCGAGTCA TTGCCGTCTT AAAAGCCCGA GAGCTGACTC CGCATGGGGT GCTGGTGGAT
GCTGCCGTGG TGGCAAAGGA CACTGTTGTG TGCACCTTTG AAAATGAAAA CACAGAGTGG
TGCCTGGCCG TCTGGAGGGG CTGGGGCGCC AGGGAGTTCG ACATTTTCTA CCAGTCCTAC
GAGGAGCTGG GCCGGCTGGA GGCTTGCACT CGCAAGAGAA GGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520318.1
CDS138..5882
Misc Feature(1)210..497
Misc Feature(2)210..>488
Misc Feature(3)order(210..212,219..227,231..236,246..248,255..257,294..296,300..302,306..311,318..326,330..335,345..347,357..359,390..392,396..398,417..419,429..434,441..449,453..458,468..470,477..479)
Misc Feature(4)300..392
Misc Feature(5)396..488
Misc Feature(6)<474..1298
Misc Feature(7)675..743
Misc Feature(8)738..1676
Misc Feature(9)744..824
Misc Feature(10)825..893
Misc Feature(11)894..977
Misc Feature(12)969..1757
Misc Feature(13)978..1049
Misc Feature(14)order(996..1001,1134..1136,1350..1352,1422..1424,1572..1574,1626..1628,1656..1658)
Misc Feature(15)1050..1187
Misc Feature(16)1188..1256
Misc Feature(17)1257..1328
Misc Feature(18)1329..1481
Misc Feature(19)1482..1550
Misc Feature(20)1737..>2177
Misc Feature(21)1749..2297
Misc Feature(22)1767..1790
Misc Feature(23)order(1773..1793,1938..1940,2103..2108,2112..2114,2247..2255)
Misc Feature(24)1833..1835
Misc Feature(25)1854..1862
Misc Feature(26)1929..1940
Misc Feature(27)order(1935..1940,1986..1991)
Misc Feature(28)2103..2114
Misc Feature(29)2247..2255
Misc Feature(30)2364..2942
Misc Feature(31)3567..4367
Misc Feature(32)3576..4403
Misc Feature(33)order(3576..3590,3600..3602,3636..3638,3642..3644,3792..3794,3834..3845,3855..3857,3861..3863,3990..3992,3996..3998,4002..4007,4011..4013,4059..4061,4068..4070,4110..4121)
Misc Feature(34)order(3576..3590,3600..3602,3636..3638,3642..3644,3792..3794,3834..3845,3855..3857,3990..3992,3996..3998,4002..4007,4011..4013,4059..4061)
Misc Feature(35)order(3588..3590,3855..3857,3861..3863,3990..3992,3996..3998,4002..4004,4068..4070,4110..4121)
Misc Feature(36)4056..4121
Translation

Target ORF information:

RefSeq Version XM_011522016.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522016.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
ATGGTCCGCT ACCTACTCAG CAAGAGACTG GTGGAGCTGC CCACCGAGCC CACGGATGAC 
AACCCAGCCG TGGTGGCAGC GTATTTTGGA CACACGGCAG TTGTGCAGGA ATTGCTTGAG
TCCTTACCAG GTCCCTGCAG TCCCCAGCGG CTTCTGAACT GGATGCTGGC CTTGGCTTGC
CAGCGAGGGC ACCTGGGGGT TGTGAAGCTC CTGGTCCTGA CGCACGGGGC TGACCCGGAG
AGCTACGCTG TCAGGAAGAA TGAGTTCCCT GTCATCGTGC GCTTGCCCCT GTATGCGGCC
ATCAAGTCAG GGAATGAAGA CATTGCAATA TTCCTGCTTC GGCATGGGGC CTATTTCTGT
TCCTACATCT TGCTGGATAG TCCTGACCCC AGCAAACATC TGCTGAGAAA GTACTTCATT
GAAGCCAGTC CCTTGCCCAG CAGTTATCCG GGAAAAACAG CTCTCCGTGT GAAATGGTCC
CATCTCAGAC TGCCCTGGGT AGACCTAGAC TGGCTCATAG ACATCTCCTG CCAGATCACG
GAGCTCGACC TTTCTGCCAA CTGCCTGGCG ACCCTCCCCT CGGTTATCCC CTGGGGCCTC
ATCAATCTCC GGAAGCTGAA CCTCTCCGAC AACCACCTGG GGGAGCTGCC TGGCGTGCAG
TCATCGGACG AAATCATCTG TTCCAGGCTA CTTGAAATTG ACATTTCCAG CAACAAGTTG
TCCCACCTCC CTCCTGGATT CTTGCACCTC TCAAAACTTC AAAAACTGAC AGCTTCAAAA
AATTGTTTAG AAAAATTGTT CGAAGAAGAA AATGCCACTA ACTGGATAGG TTTACGGAAG
CTACAGGAAC TTGATATATC TGACAATAAA TTGACAGAAC TCCCTGCCCT GTTCCTTCAC
TCTTTCAAGT CCCTCAATTC TCTGAATGTC TCCAGAAACA ACCTGAAGGT GTTTCCAGAT
CCCTGGGCCT GCCCTTTGAA ATGTTGTAAA GCTTCCAGAA ATGCCCTGGA ATGTCTGCCA
GACAAAATGG CTGTCTTTTG GAAAAATCAC CTGAAGGATG TGGATTTCTC AGAAAACGCA
CTCAAAGAAG TTCCCCTGGG ACTTTTCCAG CTTGATGCCC TCATGTTCTT GAGGTTACAG
GGGAACCAGC TGGCGGCACT TCCACCTCAA GAGAAGTGGA CCTGCAGGCA GCTCAAAACC
CTGGATCTCT CCAGAAACCA ACTTGGCAAA AATGAAGATG GACTGAAAAC GAAGCGTATT
GCCTTTTTCA CCACCAGAGG TCGCCAGCGC TCCGGGACTG AGGCAGCAAG TGTGCTGGAA
TTTCCGGCCT TCCTAAGTGA GTCTTTGGAA GTCCTTTGCC TGAACGACAA CCACCTCGAC
ACAGTCCCTC CCTCGGTTTG CCTACTGAAG AGCTTATCAG AGCTCTACTT GGGAAACAAC
CCTGGCCTCC GGGAGCTCCC TCCTGAGCTG GGGCAGCTGG GCAACCTCTG GCAGCTGGAC
ACTGAAGACC TGACCATCAG CAATGTGCCT GCAGAAATCC AAAAAGAAGG CCCCAAAGCA
ATGCTGTCTT ACCTGCGTGC TCAGCTGCGG AAAGCGGAAA AGTGCAAGCT GATGAAGATG
ATCATCGTGG GTCCCCCGCG CCAGGGCAAG TCCACCCTCC TGGAGATCTT ACAGACGGGG
AGGGCCCCCC AGGTGGTGCA TGGAGAGGCC ACCATCAGGA CCACCAAGTG GGAGCTCCAG
AGGCCGGCTG GCTCGAGAGC CAAGGTTGAG TCCGTGGAGT TCAACGTCTG GGACATCGGG
GGACCGGCCA GCATGGCCAC TGTCAACCAG TGCTTCTTCA CGGACAAGGC CCTGTACGTG
GTGGTCTGGA ACCTGGCGCT GGGGGAGGAG GCCGTGGCCA ACCTCCAGTT CTGGCTGCTC
AACATCGAGG CCAAGGCCCC AAACGCCGTG GTGCTGGTGG TCGGGACGCA CCTGGATTTA
ATTGAAGCCA AGTTCCGTGT GGAAAGGATT GCAACGCTGC GTGCCTATGT GCTGGCACTC
TGCCGCTCCC CCTCCGGCTC CAGGGCCACA GGCTTCCCAG ACATCACCTT CAAACACTTA
CATGAGATTT CCTGCAAGAG CCTGGAAGGT CAGGAAGGGC TGCGACAGCT GATTTTCCAC
GTCACGTGCA GCATGAAGGA CGTGGGCAGC ACCATCGGCT GCCAGCGACT GGCAGGGCGG
CTGATCCCCA GGAGCTACCT GAGCCTGCAG GAGGCCGTGC TGGCAGAGCA GCAGCGCCGC
AGCCGGGACG ACGACGTGCA GTACCTGACG GACAGGCAGC TGGAGCAGCT GGTGGAGCAG
ACGCCCGACA ACGACATCAA GGACTACGAG GACCTGCAGT CAGCCATCAG CTTCCTCATA
GAAACCGGCA CCCTGCTCCA TTTCCCGGAC ACCAGCCACG GCCTGAGGAA CCTCTACTTC
CTCGACCCTA TTTGGCTCTC CGAATGTCTG CAGAGGATCT TTAATATTAA GGGCTCTCGG
TCAGTGGCCA AGAATGGGGT GATCAGAGCA GAAGACCTCA GGATGCTGCT GGTGGGGACT
GGCTTCACGC AGCAGACGGA AGAGCAGTAC TTCCAGTTCC TGGCCAAGTT TGAGATCGCC
CTGCCCGTCG CCAATGACAG CTACCTCCTG CCCCATCTCC TTCCATCTAA ACCTGGCCTG
GACACCCACG GTATGCGGCA CCCCACAGCC AACACCATTC AGAGGGTATT TAAGATGAGC
TTCGTTCCCG TTGGCTTCTG GCAAAGGTTT ATAGCACGGA TGCTGATCAG CCTGGCGGAG
ATGGACCTGC AGCTTTTTGA AAACAAGAAG AATACTAAAA GCAGGAACAG GAAAGTCACC
ATTTACAGTT TTACAGGAAA CCAGAGAAAT CGCTGTAGCA CATTCAGAGT GAAAAGAAAT
CAGACCATCT ATTGGCAGGA AGGGCTCCTG GTCACTTTTG ATGGGGGCTA CCTCAGTGTG
GAATCTTCCG ACGTGAACTG GAAAAAGAAG AAAAGCGGAG GAATGAAAAT TGTTTGCCAA
TCAGAAGTGA GGGACTTCTC AGCCATGGCT TTCATCACGG ACCACGTCAA TTCCTTGATT
GATCAGTGGT TTCCCGCCCT GACAGCCACA GAGAGCGACG GGACGCCACT CATGGAGCAG
TACGTGCCCT GCCCGGTCTG CGAGACAGCC TGGGCCCAGC ACACGGACCC CAGTGAGAAA
TCAGAGGATG TGCAGTACTT CGACATGGAA GACTGTGTCC TGACGGCCAT CGAGCGGGAC
TTCATCTCCT GCCCCAGACA CCCGGACCTC CCCGTGCCGC TGCAGGAGCT GGTCCCTGAA
CTGTTCATGA CCGACTTCCC GGCCAGGCTC TTCCTGGAGA ACAGCAAGCT GGAGCACAGC
GAGGACGAGG GCAGCGTCCT GGGCCAGGGC GGCAGTGGCA CCGTCATCTA CCGGGCCCGG
TACCAGGGCC AGCCTGTGGC CGTCAAGCGC TTCCACATCA AAAAATTCAA GAACTTTGCT
AACGTACCGG CAGACACCAT GCTGAGGCAC CTGCGGGCCA CCGATGCCAT GAAGAACTTC
TCCGAGTTCC GGCAGGAGGC CAGCATGCTG CACGCGCTGC AGCACCCCTG CATCGTGGCG
CTCATCGGCA TCAGCATCCA CCCGCTCTGC TTCGCCCTGG AGCTCGCGCC GCTCAGCAGC
CTCAACACCG TGCTGTCCGA GAACGCCAGA GATTCTTCCT TTATACCCCT GGGACACATG
CTCACCCAAA AAATAGCCTA CCAGATCGCC TCGGGCCTGG CCTACCTGCA CAAGAAAAAC
ATCATCTTCT GTGACCTGAA GTCGGACAAC ATTCTGGTGT GGTCCCTTGA CGTCAAGGAG
CACATCAACA TCAAGCTATC TGACTACGGG ATTTCGAGGC AGTCATTCCA TGAGGGCGCC
CTAGGCGTGG AGGGCACTCC TGGCTACCAG GCCCCAGAGA TCAGGCCTCG CATTGTATAT
GATGAGAAGG TAGATATGTT CTCCTATGGA ATGGTGCTCT ACGAGTTGCT GTCAGGACAG
CGCCCTGCAC TGGGCCACCA CCAGCTCCAG ATTGCCAAGA AGCTGTCCAA GGGCATCCGC
CCGGTTCTGG GGCAGCCGGA GGAAGTGCAG TTCCGGCGAC TGCAGGCGCT CATGATGGAG
TGCTGGGACA CTAAGCCAGA GAAGCGACCG CTGGCCCTGT CGGTGGTGAG CCAGATGAAG
GACCCGACTT TTGCCACCTT CATGTATGAA CTGTGCTGTG GGAAGCAGAC AGCCTTCTTC
TCATCCCAGG GCCAGGAGTA CACCGTGGTG TTTTGGGATG GAAAAGAGGA GTCCAGGAAC
TACACGGTGG TGAACACAGA GAAGGGCCTC ATGGAGGTGC AGAGGATGTG CTGCCCTGGG
ATGAAGGTGA GCTGCCAGCT CCAGGTCCAG AGATCCCTGT GGACAGCCAC CGAGGACCAG
AAAATCTACA TCTACACCCT CAAGGGCATG TGCCCCTTAA ACACACCCCA ACAGGCCTTG
GATACTCCAG CTGTCGTCAC CTGCTTCTTG GCCGTGCCTG TTATTAAAAA GAATTCCTAC
CTGGTCTTAG CGGGCCTCGC CGATGGGCTT GTGGCTGTGT TTCCCGTGGT GCGGGGCACC
CCAAAGGACA GCTGCTCCTA CCTGTGCTCA CACACAGCCA ACAGGTCCAA GTTCAGCATC
GCGGATGAAG ACGCACGGCA GAACCCCTAC CCAGTGAAGG CCATGGAGGT GGTCAACAGC
GGCTCTGAGG TCTGGTACAG CAATGGGCCG GGCCTCCTTG TCATCGACTG TGCCTCCCTG
GAGATCTGCA GGCGGCTGGA GCCCTACATG GCCCCCTCCA TGGTTACGTC AGTCGTGTGC
AGCTCTGAGG GCAGAGGGGA GGAGGTCGTC TGGTGCCTGG ATGACAAGGC CAACTCCTTG
GTGATGTACC ACTCCACCAC CTACCAGCTG TGTGCCCGGT ACTTCTGCGG GGTCCCCAGC
CCCCTCAGGG ACATGTTTCC CGTGCGGCCC TTGGACACGG AACCCCCGGC AGCCAGCCAC
ACGGCCAACC CAAAGGTGCC TGAGGGGGAC TCCATCGCGG ACGTGAGCAT CATGTACAGT
GAGGAGCTGG GCACGCAGAT CCTGATCCAC CAGGAATCAC TCACTGACTA CTGCTCCATG
TCCTCCTACT CCTCATCCCC ACCCCGCCAG GCTGCCAGGT CCCCCTCAAG CCTCCCCAGC
TCCCCAGCAA GTTCTTCCAG TGTGCCTTTC TCCACCGACT GCGAGGACTC AGACATGCTA
CATACGCCCG GTGCTGCCTC CGACAGGTCT GAGCATGACC TGACCCCCAT GGACGGGGAG
ACCTTCAGCC AGCACCTGCA GGCCGTGAAG ATCCTCGCCG TCAGAGACCT CATTTGGGTC
CCCAGGCGCG GTGGAGATGT TATCGTCATT GGCCTGGAGA AGGATTCTGG CGCCCAGCGG
GGCCGAGTCA TTGCCGTCTT AAAAGCCCGA GAGCTGACTC CGCATGGGGT GCTGGTGGAT
GCTGCCGTGG TGGCAAAGGA CACTGTTGTG TGCACCTTTG AAAATGAAAA CACAGAGTGG
TGCCTGGCCG TCTGGAGGGG CTGGGGCGCC AGGGAGTTCG ACATTTTCTA CCAGTCCTAC
GAGGAGCTGG GCCGGCTGGA GGCTTGCACT CGCAAGAGAA GGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72031
Clone ID Related Accession (Same CDS sequence) XM_011522012.2 , NM_024652.5 , XM_011522013.2
Accession Version NM_024652.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-24
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AW592808.1, BC171802.1, AC090907.14, DQ013130.1, AK131075.1, AK058028.1, BQ187037.1 and AC019254.12. This sequence is a reference standard in the RefSeqGene project. On Jan 5, 2017 this sequence version replaced NM_024652.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGC GCGGTGGAGA TGTTATCGTC ATTGGCCTGG AGAAGGATTC TGGCGCCCAG
CGGGGCCGAG TCATTGCCGT CTTAAAAGCC CGAGAGCTGA CTCCGCATGG GGTGCTGGTG
GATGCTGCCG TGGTGGCAAA GGACACTGTT GTGTGCACCT TTGAAAATGA AAACACAGAG
TGGTGCCTGG CCGTCTGGAG GGGCTGGGGC GCCAGGGAGT TCGACATTTT CTACCAGTCC
TACGAGGAGC TGGGCCGGCT GGAGGCTTGC ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_078928.3
CDS360..6407
Misc Feature(1)282..284
Misc Feature(2)615..707
Misc Feature(3)642..1001
Misc Feature(4)714..803
Misc Feature(5)735..1022
Misc Feature(6)order(735..737,744..752,756..761,771..773,780..782,819..821,825..827,831..836,843..851,855..860,870..872,882..884,915..917,921..923,942..944,954..959,966..974,978..983,993..995,1002..1004)
Misc Feature(7)813..905
Misc Feature(8)825..917
Misc Feature(9)921..1013
Misc Feature(10)936..1025
Misc Feature(11)<999..1823
Misc Feature(12)1194..1259
Misc Feature(13)1200..1268
Misc Feature(14)1263..2201
Misc Feature(15)1266..1331
Misc Feature(16)1269..1349
Misc Feature(17)1347..1412
Misc Feature(18)1350..1418
Misc Feature(19)1416..1481
Misc Feature(20)1419..1502
Misc Feature(21)1494..2282
Misc Feature(22)1500..1565
Misc Feature(23)1503..1574
Misc Feature(24)order(1521..1526,1659..1661,1875..1877,1947..1949,2097..2099,2151..2153,2181..2183)
Misc Feature(25)1572..1637
Misc Feature(26)1575..1712
Misc Feature(27)1638..1700
Misc Feature(28)1710..1775
Misc Feature(29)1713..1781
Misc Feature(30)1779..1844
Misc Feature(31)1782..1853
Misc Feature(32)1851..1916
Misc Feature(33)1854..2006
Misc Feature(34)2004..2069
Misc Feature(35)2007..2075
Misc Feature(36)2073..2141
Misc Feature(37)2145..2210
Misc Feature(38)2262..>2702
Misc Feature(39)2274..2822
Misc Feature(40)2292..2315
Misc Feature(41)order(2298..2318,2463..2465,2628..2633,2637..2639,2772..2780)
Misc Feature(42)2358..2360
Misc Feature(43)2379..2387
Misc Feature(44)2454..2465
Misc Feature(45)order(2460..2465,2511..2516)
Misc Feature(46)2628..2639
Misc Feature(47)2772..2780
Misc Feature(48)2889..3467
Misc Feature(49)4092..4892
Misc Feature(50)4101..4928
Misc Feature(51)order(4101..4115,4125..4127,4161..4163,4167..4169,4317..4319,4359..4370,4380..4382,4386..4388,4515..4517,4521..4523,4527..4532,4536..4538,4584..4586,4593..4595,4635..4646)
Misc Feature(52)order(4101..4115,4125..4127,4161..4163,4167..4169,4317..4319,4359..4370,4380..4382,4515..4517,4521..4523,4527..4532,4536..4538,4584..4586)
Misc Feature(53)order(4113..4115,4380..4382,4386..4388,4515..4517,4521..4523,4527..4529,4593..4595,4635..4646)
Misc Feature(54)4581..4646
Exon (1)1..237
Exon (2)238..456
Exon (3)457..620
Exon (4)621..792
Exon (5)793..972
Exon (6)973..1121
Exon (7)1122..1348
Exon (8)1349..1476
Exon (9)1477..1640
Exon (10)1641..1778
Exon (11)1779..1891
Exon (12)1892..1968
Exon (13)1969..2098
Exon (14)2099..2211
Exon (15)2212..2426
Exon (16)2427..2591
Exon (17)2592..2764
Exon (18)2765..2885
Exon (19)2886..3045
Exon (20)3046..3322
Exon (21)3323..3494
Exon (22)3495..3658
Exon (23)3659..3798
Exon (24)3799..4048
Exon (25)4049..4215
Exon (26)4216..4413
Exon (27)4414..4691
Exon (28)4692..4886
Exon (29)4887..5038
Exon (30)5039..5156
Exon (31)5157..5273
Exon (32)5274..6127
Exon (33)6128..6229
Exon (34)6230..10449
Translation

Target ORF information:

RefSeq Version NM_024652.5
Organism Homo sapiens(Human)
Definition Homo sapiens leucine rich repeat kinase 1 (LRRK1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_024652.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGC GCGGTGGAGA TGTTATCGTC ATTGGCCTGG AGAAGGATTC TGGCGCCCAG
CGGGGCCGAG TCATTGCCGT CTTAAAAGCC CGAGAGCTGA CTCCGCATGG GGTGCTGGTG
GATGCTGCCG TGGTGGCAAA GGACACTGTT GTGTGCACCT TTGAAAATGA AAACACAGAG
TGGTGCCTGG CCGTCTGGAG GGGCTGGGGC GCCAGGGAGT TCGACATTTT CTACCAGTCC
TACGAGGAGC TGGGCCGGCT GGAGGCTTGC ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72031
Clone ID Related Accession (Same CDS sequence) XM_011522012.2 , NM_024652.5 , XM_011522013.2
Accession Version XM_011522012.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGC GCGGTGGAGA TGTTATCGTC ATTGGCCTGG AGAAGGATTC TGGCGCCCAG
CGGGGCCGAG TCATTGCCGT CTTAAAAGCC CGAGAGCTGA CTCCGCATGG GGTGCTGGTG
GATGCTGCCG TGGTGGCAAA GGACACTGTT GTGTGCACCT TTGAAAATGA AAACACAGAG
TGGTGCCTGG CCGTCTGGAG GGGCTGGGGC GCCAGGGAGT TCGACATTTT CTACCAGTCC
TACGAGGAGC TGGGCCGGCT GGAGGCTTGC ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520314.1
CDS634..6681
Misc Feature(1)916..1275
Misc Feature(2)1009..1296
Misc Feature(3)order(1009..1011,1018..1026,1030..1035,1045..1047,1054..1056,1093..1095,1099..1101,1105..1110,1117..1125,1129..1134,1144..1146,1156..1158,1189..1191,1195..1197,1216..1218,1228..1233,1240..1248,1252..1257,1267..1269,1276..1278)
Misc Feature(4)1099..1191
Misc Feature(5)1195..1287
Misc Feature(6)<1273..2097
Misc Feature(7)1474..1542
Misc Feature(8)1537..2475
Misc Feature(9)1543..1623
Misc Feature(10)1624..1692
Misc Feature(11)1693..1776
Misc Feature(12)1768..2556
Misc Feature(13)1777..1848
Misc Feature(14)order(1795..1800,1933..1935,2149..2151,2221..2223,2371..2373,2425..2427,2455..2457)
Misc Feature(15)1849..1986
Misc Feature(16)1987..2055
Misc Feature(17)2056..2127
Misc Feature(18)2128..2280
Misc Feature(19)2281..2349
Misc Feature(20)2536..>2976
Misc Feature(21)2548..3096
Misc Feature(22)2566..2589
Misc Feature(23)order(2572..2592,2737..2739,2902..2907,2911..2913,3046..3054)
Misc Feature(24)2632..2634
Misc Feature(25)2653..2661
Misc Feature(26)2728..2739
Misc Feature(27)order(2734..2739,2785..2790)
Misc Feature(28)2902..2913
Misc Feature(29)3046..3054
Misc Feature(30)3163..3741
Misc Feature(31)4366..5166
Misc Feature(32)4375..5202
Misc Feature(33)order(4375..4389,4399..4401,4435..4437,4441..4443,4591..4593,4633..4644,4654..4656,4660..4662,4789..4791,4795..4797,4801..4806,4810..4812,4858..4860,4867..4869,4909..4920)
Misc Feature(34)order(4375..4389,4399..4401,4435..4437,4441..4443,4591..4593,4633..4644,4654..4656,4789..4791,4795..4797,4801..4806,4810..4812,4858..4860)
Misc Feature(35)order(4387..4389,4654..4656,4660..4662,4789..4791,4795..4797,4801..4803,4867..4869,4909..4920)
Misc Feature(36)4855..4920
Translation

Target ORF information:

RefSeq Version XM_011522012.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522012.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGC GCGGTGGAGA TGTTATCGTC ATTGGCCTGG AGAAGGATTC TGGCGCCCAG
CGGGGCCGAG TCATTGCCGT CTTAAAAGCC CGAGAGCTGA CTCCGCATGG GGTGCTGGTG
GATGCTGCCG TGGTGGCAAA GGACACTGTT GTGTGCACCT TTGAAAATGA AAACACAGAG
TGGTGCCTGG CCGTCTGGAG GGGCTGGGGC GCCAGGGAGT TCGACATTTT CTACCAGTCC
TACGAGGAGC TGGGCCGGCT GGAGGCTTGC ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72031
Clone ID Related Accession (Same CDS sequence) XM_011522012.2 , NM_024652.5 , XM_011522013.2
Accession Version XM_011522013.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011522013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGC GCGGTGGAGA TGTTATCGTC ATTGGCCTGG AGAAGGATTC TGGCGCCCAG
CGGGGCCGAG TCATTGCCGT CTTAAAAGCC CGAGAGCTGA CTCCGCATGG GGTGCTGGTG
GATGCTGCCG TGGTGGCAAA GGACACTGTT GTGTGCACCT TTGAAAATGA AAACACAGAG
TGGTGCCTGG CCGTCTGGAG GGGCTGGGGC GCCAGGGAGT TCGACATTTT CTACCAGTCC
TACGAGGAGC TGGGCCGGCT GGAGGCTTGC ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520315.1
CDS343..6390
Misc Feature(1)625..984
Misc Feature(2)718..1005
Misc Feature(3)order(718..720,727..735,739..744,754..756,763..765,802..804,808..810,814..819,826..834,838..843,853..855,865..867,898..900,904..906,925..927,937..942,949..957,961..966,976..978,985..987)
Misc Feature(4)808..900
Misc Feature(5)904..996
Misc Feature(6)<982..1806
Misc Feature(7)1183..1251
Misc Feature(8)1246..2184
Misc Feature(9)1252..1332
Misc Feature(10)1333..1401
Misc Feature(11)1402..1485
Misc Feature(12)1477..2265
Misc Feature(13)1486..1557
Misc Feature(14)order(1504..1509,1642..1644,1858..1860,1930..1932,2080..2082,2134..2136,2164..2166)
Misc Feature(15)1558..1695
Misc Feature(16)1696..1764
Misc Feature(17)1765..1836
Misc Feature(18)1837..1989
Misc Feature(19)1990..2058
Misc Feature(20)2245..>2685
Misc Feature(21)2257..2805
Misc Feature(22)2275..2298
Misc Feature(23)order(2281..2301,2446..2448,2611..2616,2620..2622,2755..2763)
Misc Feature(24)2341..2343
Misc Feature(25)2362..2370
Misc Feature(26)2437..2448
Misc Feature(27)order(2443..2448,2494..2499)
Misc Feature(28)2611..2622
Misc Feature(29)2755..2763
Misc Feature(30)2872..3450
Misc Feature(31)4075..4875
Misc Feature(32)4084..4911
Misc Feature(33)order(4084..4098,4108..4110,4144..4146,4150..4152,4300..4302,4342..4353,4363..4365,4369..4371,4498..4500,4504..4506,4510..4515,4519..4521,4567..4569,4576..4578,4618..4629)
Misc Feature(34)order(4084..4098,4108..4110,4144..4146,4150..4152,4300..4302,4342..4353,4363..4365,4498..4500,4504..4506,4510..4515,4519..4521,4567..4569)
Misc Feature(35)order(4096..4098,4363..4365,4369..4371,4498..4500,4504..4506,4510..4512,4576..4578,4618..4629)
Misc Feature(36)4564..4629
Translation

Target ORF information:

RefSeq Version XM_011522013.2
Organism Homo sapiens(Human)
Definition Homo sapiens leucine-rich repeat kinase 1 (LRRK1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011522013.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
ATGGCTGGCA TGTCGCAAAG ACCCCCCAGC ATGTACTGGT GTGTGGGGCC GGAGGAGTCA 
GCTGTGTGTC CAGAACGTGC CATGGAGACG CTTAACGGTG CCGGGGACAC GGGCGGCAAG
CCGTCCACGC GGGGCGGTGA CCCTGCAGCG CGGTCCCGCA GGACGGAAGG CATCCGCGCC
GCGTACAGGC GGGGAGACCG CGGCGGCGCC CGGGACCTGC TGGAGGAGGC CTGCGACCAG
TGCGCGTCCC AGCTGGAAAA GGGCCAGCTT CTGAGCATCC CGGCAGCCTA TGGGGATCTG
GAGATGGTCC GCTACCTACT CAGCAAGAGA CTGGTGGAGC TGCCCACCGA GCCCACGGAT
GACAACCCAG CCGTGGTGGC AGCGTATTTT GGACACACGG CAGTTGTGCA GGAATTGCTT
GAGTCCTTAC CAGGTCCCTG CAGTCCCCAG CGGCTTCTGA ACTGGATGCT GGCCTTGGCT
TGCCAGCGAG GGCACCTGGG GGTTGTGAAG CTCCTGGTCC TGACGCACGG GGCTGACCCG
GAGAGCTACG CTGTCAGGAA GAATGAGTTC CCTGTCATCG TGCGCTTGCC CCTGTATGCG
GCCATCAAGT CAGGGAATGA AGACATTGCA ATATTCCTGC TTCGGCATGG GGCCTATTTC
TGTTCCTACA TCTTGCTGGA TAGTCCTGAC CCCAGCAAAC ATCTGCTGAG AAAGTACTTC
ATTGAAGCCA GTCCCTTGCC CAGCAGTTAT CCGGGAAAAA CAGCTCTCCG TGTGAAATGG
TCCCATCTCA GACTGCCCTG GGTAGACCTA GACTGGCTCA TAGACATCTC CTGCCAGATC
ACGGAGCTCG ACCTTTCTGC CAACTGCCTG GCGACCCTCC CCTCGGTTAT CCCCTGGGGC
CTCATCAATC TCCGGAAGCT GAACCTCTCC GACAACCACC TGGGGGAGCT GCCTGGCGTG
CAGTCATCGG ACGAAATCAT CTGTTCCAGG CTACTTGAAA TTGACATTTC CAGCAACAAG
TTGTCCCACC TCCCTCCTGG ATTCTTGCAC CTCTCAAAAC TTCAAAAACT GACAGCTTCA
AAAAATTGTT TAGAAAAATT GTTCGAAGAA GAAAATGCCA CTAACTGGAT AGGTTTACGG
AAGCTACAGG AACTTGATAT ATCTGACAAT AAATTGACAG AACTCCCTGC CCTGTTCCTT
CACTCTTTCA AGTCCCTCAA TTCTCTGAAT GTCTCCAGAA ACAACCTGAA GGTGTTTCCA
GATCCCTGGG CCTGCCCTTT GAAATGTTGT AAAGCTTCCA GAAATGCCCT GGAATGTCTG
CCAGACAAAA TGGCTGTCTT TTGGAAAAAT CACCTGAAGG ATGTGGATTT CTCAGAAAAC
GCACTCAAAG AAGTTCCCCT GGGACTTTTC CAGCTTGATG CCCTCATGTT CTTGAGGTTA
CAGGGGAACC AGCTGGCGGC ACTTCCACCT CAAGAGAAGT GGACCTGCAG GCAGCTCAAA
ACCCTGGATC TCTCCAGAAA CCAACTTGGC AAAAATGAAG ATGGACTGAA AACGAAGCGT
ATTGCCTTTT TCACCACCAG AGGTCGCCAG CGCTCCGGGA CTGAGGCAGC AAGTGTGCTG
GAATTTCCGG CCTTCCTAAG TGAGTCTTTG GAAGTCCTTT GCCTGAACGA CAACCACCTC
GACACAGTCC CTCCCTCGGT TTGCCTACTG AAGAGCTTAT CAGAGCTCTA CTTGGGAAAC
AACCCTGGCC TCCGGGAGCT CCCTCCTGAG CTGGGGCAGC TGGGCAACCT CTGGCAGCTG
GACACTGAAG ACCTGACCAT CAGCAATGTG CCTGCAGAAA TCCAAAAAGA AGGCCCCAAA
GCAATGCTGT CTTACCTGCG TGCTCAGCTG CGGAAAGCGG AAAAGTGCAA GCTGATGAAG
ATGATCATCG TGGGTCCCCC GCGCCAGGGC AAGTCCACCC TCCTGGAGAT CTTACAGACG
GGGAGGGCCC CCCAGGTGGT GCATGGAGAG GCCACCATCA GGACCACCAA GTGGGAGCTC
CAGAGGCCGG CTGGCTCGAG AGCCAAGGTT GAGTCCGTGG AGTTCAACGT CTGGGACATC
GGGGGACCGG CCAGCATGGC CACTGTCAAC CAGTGCTTCT TCACGGACAA GGCCCTGTAC
GTGGTGGTCT GGAACCTGGC GCTGGGGGAG GAGGCCGTGG CCAACCTCCA GTTCTGGCTG
CTCAACATCG AGGCCAAGGC CCCAAACGCC GTGGTGCTGG TGGTCGGGAC GCACCTGGAT
TTAATTGAAG CCAAGTTCCG TGTGGAAAGG ATTGCAACGC TGCGTGCCTA TGTGCTGGCA
CTCTGCCGCT CCCCCTCCGG CTCCAGGGCC ACAGGCTTCC CAGACATCAC CTTCAAACAC
TTACATGAGA TTTCCTGCAA GAGCCTGGAA GGTCAGGAAG GGCTGCGACA GCTGATTTTC
CACGTCACGT GCAGCATGAA GGACGTGGGC AGCACCATCG GCTGCCAGCG ACTGGCAGGG
CGGCTGATCC CCAGGAGCTA CCTGAGCCTG CAGGAGGCCG TGCTGGCAGA GCAGCAGCGC
CGCAGCCGGG ACGACGACGT GCAGTACCTG ACGGACAGGC AGCTGGAGCA GCTGGTGGAG
CAGACGCCCG ACAACGACAT CAAGGACTAC GAGGACCTGC AGTCAGCCAT CAGCTTCCTC
ATAGAAACCG GCACCCTGCT CCATTTCCCG GACACCAGCC ACGGCCTGAG GAACCTCTAC
TTCCTCGACC CTATTTGGCT CTCCGAATGT CTGCAGAGGA TCTTTAATAT TAAGGGCTCT
CGGTCAGTGG CCAAGAATGG GGTGATCAGA GCAGAAGACC TCAGGATGCT GCTGGTGGGG
ACTGGCTTCA CGCAGCAGAC GGAAGAGCAG TACTTCCAGT TCCTGGCCAA GTTTGAGATC
GCCCTGCCCG TCGCCAATGA CAGCTACCTC CTGCCCCATC TCCTTCCATC TAAACCTGGC
CTGGACACCC ACGGTATGCG GCACCCCACA GCCAACACCA TTCAGAGGGT ATTTAAGATG
AGCTTCGTTC CCGTTGGCTT CTGGCAAAGG TTTATAGCAC GGATGCTGAT CAGCCTGGCG
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACTA AAAGCAGGAA CAGGAAAGTC
ACCATTTACA GTTTTACAGG AAACCAGAGA AATCGCTGTA GCACATTCAG AGTGAAAAGA
AATCAGACCA TCTATTGGCA GGAAGGGCTC CTGGTCACTT TTGATGGGGG CTACCTCAGT
GTGGAATCTT CCGACGTGAA CTGGAAAAAG AAGAAAAGCG GAGGAATGAA AATTGTTTGC
CAATCAGAAG TGAGGGACTT CTCAGCCATG GCTTTCATCA CGGACCACGT CAATTCCTTG
ATTGATCAGT GGTTTCCCGC CCTGACAGCC ACAGAGAGCG ACGGGACGCC ACTCATGGAG
CAGTACGTGC CCTGCCCGGT CTGCGAGACA GCCTGGGCCC AGCACACGGA CCCCAGTGAG
AAATCAGAGG ATGTGCAGTA CTTCGACATG GAAGACTGTG TCCTGACGGC CATCGAGCGG
GACTTCATCT CCTGCCCCAG ACACCCGGAC CTCCCCGTGC CGCTGCAGGA GCTGGTCCCT
GAACTGTTCA TGACCGACTT CCCGGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC
AGCGAGGACG AGGGCAGCGT CCTGGGCCAG GGCGGCAGTG GCACCGTCAT CTACCGGGCC
CGGTACCAGG GCCAGCCTGT GGCCGTCAAG CGCTTCCACA TCAAAAAATT CAAGAACTTT
GCTAACGTAC CGGCAGACAC CATGCTGAGG CACCTGCGGG CCACCGATGC CATGAAGAAC
TTCTCCGAGT TCCGGCAGGA GGCCAGCATG CTGCACGCGC TGCAGCACCC CTGCATCGTG
GCGCTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCC TGGAGCTCGC GCCGCTCAGC
AGCCTCAACA CCGTGCTGTC CGAGAACGCC AGAGATTCTT CCTTTATACC CCTGGGACAC
ATGCTCACCC AAAAAATAGC CTACCAGATC GCCTCGGGCC TGGCCTACCT GCACAAGAAA
AACATCATCT TCTGTGACCT GAAGTCGGAC AACATTCTGG TGTGGTCCCT TGACGTCAAG
GAGCACATCA ACATCAAGCT ATCTGACTAC GGGATTTCGA GGCAGTCATT CCATGAGGGC
GCCCTAGGCG TGGAGGGCAC TCCTGGCTAC CAGGCCCCAG AGATCAGGCC TCGCATTGTA
TATGATGAGA AGGTAGATAT GTTCTCCTAT GGAATGGTGC TCTACGAGTT GCTGTCAGGA
CAGCGCCCTG CACTGGGCCA CCACCAGCTC CAGATTGCCA AGAAGCTGTC CAAGGGCATC
CGCCCGGTTC TGGGGCAGCC GGAGGAAGTG CAGTTCCGGC GACTGCAGGC GCTCATGATG
GAGTGCTGGG ACACTAAGCC AGAGAAGCGA CCGCTGGCCC TGTCGGTGGT GAGCCAGATG
AAGGACCCGA CTTTTGCCAC CTTCATGTAT GAACTGTGCT GTGGGAAGCA GACAGCCTTC
TTCTCATCCC AGGGCCAGGA GTACACCGTG GTGTTTTGGG ATGGAAAAGA GGAGTCCAGG
AACTACACGG TGGTGAACAC AGAGAAGGGC CTCATGGAGG TGCAGAGGAT GTGCTGCCCT
GGGATGAAGG TGAGCTGCCA GCTCCAGGTC CAGAGATCCC TGTGGACAGC CACCGAGGAC
CAGAAAATCT ACATCTACAC CCTCAAGGGC ATGTGCCCCT TAAACACACC CCAACAGGCC
TTGGATACTC CAGCTGTCGT CACCTGCTTC TTGGCCGTGC CTGTTATTAA AAAGAATTCC
TACCTGGTCT TAGCGGGCCT CGCCGATGGG CTTGTGGCTG TGTTTCCCGT GGTGCGGGGC
ACCCCAAAGG ACAGCTGCTC CTACCTGTGC TCACACACAG CCAACAGGTC CAAGTTCAGC
ATCGCGGATG AAGACGCACG GCAGAACCCC TACCCAGTGA AGGCCATGGA GGTGGTCAAC
AGCGGCTCTG AGGTCTGGTA CAGCAATGGG CCGGGCCTCC TTGTCATCGA CTGTGCCTCC
CTGGAGATCT GCAGGCGGCT GGAGCCCTAC ATGGCCCCCT CCATGGTTAC GTCAGTCGTG
TGCAGCTCTG AGGGCAGAGG GGAGGAGGTC GTCTGGTGCC TGGATGACAA GGCCAACTCC
TTGGTGATGT ACCACTCCAC CACCTACCAG CTGTGTGCCC GGTACTTCTG CGGGGTCCCC
AGCCCCCTCA GGGACATGTT TCCCGTGCGG CCCTTGGACA CGGAACCCCC GGCAGCCAGC
CACACGGCCA ACCCAAAGGT GCCTGAGGGG GACTCCATCG CGGACGTGAG CATCATGTAC
AGTGAGGAGC TGGGCACGCA GATCCTGATC CACCAGGAAT CACTCACTGA CTACTGCTCC
ATGTCCTCCT ACTCCTCATC CCCACCCCGC CAGGCTGCCA GGTCCCCCTC AAGCCTCCCC
AGCTCCCCAG CAAGTTCTTC CAGTGTGCCT TTCTCCACCG ACTGCGAGGA CTCAGACATG
CTACATACGC CCGGTGCTGC CTCCGACAGG TCTGAGCATG ACCTGACCCC CATGGACGGG
GAGACCTTCA GCCAGCACCT GCAGGCCGTG AAGATCCTCG CCGTCAGAGA CCTCATTTGG
GTCCCCAGGC GCGGTGGAGA TGTTATCGTC ATTGGCCTGG AGAAGGATTC TGGCGCCCAG
CGGGGCCGAG TCATTGCCGT CTTAAAAGCC CGAGAGCTGA CTCCGCATGG GGTGCTGGTG
GATGCTGCCG TGGTGGCAAA GGACACTGTT GTGTGCACCT TTGAAAATGA AAACACAGAG
TGGTGCCTGG CCGTCTGGAG GGGCTGGGGC GCCAGGGAGT TCGACATTTT CTACCAGTCC
TACGAGGAGC TGGGCCGGCT GGAGGCTTGC ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of biallelic LRRK1 mutations in osteosclerotic metaphyseal dysplasia and evidence for locus heterogeneity.
Journal of medical genetics53(8)568-74(2016 Aug)
Iida A,Xing W,Docx MK,Nakashima T,Wang Z,Kimizuka M,Van Hul W,Rating D,Spranger J,Ohashi H,Miyake N,Matsumoto N,Mohan S,Nishimura G,Mortier G,Ikegawa S


book

PLK1-dependent activation of LRRK1 regulates spindle orientation by phosphorylating CDK5RAP2.
Nature cell biology17(8)1024-35(2015 Aug)
Hanafusa H,Kedashiro S,Tezuka M,Funatsu M,Usami S,Toyoshima F,Matsumoto K


book

LRRK1-phosphorylated CLIP-170 regulates EGFR trafficking by recruiting p150Glued to microtubule plus ends.
Journal of cell science128(2)385-96(2015 Jan)
Kedashiro S,Pastuhov SI,Nishioka T,Watanabe T,Kaibuchi K,Matsumoto K,Hanafusa H


book

Differential protein-protein interactions of LRRK1 and LRRK2 indicate roles in distinct cellular signaling pathways.
Journal of neurochemistry131(2)239-50(2014 Oct)
Reyniers L,Del Giudice MG,Civiero L,Belluzzi E,Lobbestael E,Beilina A,Arrigoni G,Derua R,Waelkens E,Li Y,Crosio C,Iaccarino C,Cookson MR,Baekelandt V,Greggio E,Taymans JM


book

Unbiased screen for interactors of leucine-rich repeat kinase 2 supports a common pathway for sporadic and familial Parkinson disease.
Proceedings of the National Academy of Sciences of the United States of America111(7)2626-31(2014 Feb)
Beilina A,Rudenko IN,Kaganovich A,Civiero L,Chau H,Kalia SK,Kalia LV,Lobbestael E,Chia R,Ndukwe K,Ding J,Nalls MA,,,Olszewski M,Hauser DN,Kumaran R,Lozano AM,Baekelandt V,Greene LE,Taymans JM,Greggio E,Cookson MR


 
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