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COL18A1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol COL18A1
Entrez Gene ID 80781
Full Name collagen type XVIII alpha 1 chain
Synonyms KNO,KNO1,KS
General protein information
Preferred Names

collagen type XVIII alpha 1 chain

Names

collagen alpha-1(XVIII) chain
antiangiogenic agent
collagen alpha-1(XVIII) chain isoform 1 preproprotein
collagen, type XVIII, alpha 1
endostatin
multi-functional protein MFP

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

21

21q22.3

Summary This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014].
Disorder MIM:

120328

Disorder Html: Knobloch syndrome, type 1, 267750 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_030582.3 NP_085059.2 collagen alpha-1(XVIII) chain isoform 1 preproprotein
NM_130444.2 NP_569711.2 collagen alpha-1(XVIII) chain isoform 3 preproprotein
NM_130445.3 NP_569712.2 collagen alpha-1(XVIII) chain isoform 2 preproprotein



Rattus norvegicus (Norway rat) Col18a1 NP_445941.2
Pan troglodytes (chimpanzee) COL18A1 XP_531500.4
Macaca mulatta (rhesus monkey) COL18A1 XP_002803149.1
Homo sapiens (human) COL18A1 NP_085059.2
Mus musculus (house mouse) Col18a1 NP_001103461.1
Xenopus tropicalis (tropical clawed frog) col18a1 NP_989236.1
Gallus gallus (chicken) COL18A1 NP_989495.1


Related articles in PubMed

Endostatin is higher and associated with pulmonary involvement in primary Sjögren's syndrome.
Palm Ø, Ueland T, Garen T, Michelsen AE, Reiseter S, Aukrust P, Aaløkken TM, Molberg Ø
Clinical and experimental rheumatology34(4)690-3(2016 Jul-Aug)

Homozygosity mapping and whole exome sequencing reveal a novel homozygous COL18A1 mutation causing Knobloch syndrome.
Haghighi A, Tiwari A, Piri N, Nürnberg G, Saleh-Gohari N, Haghighi A, Neidhardt J, Nürnberg P, Berger W
PloS one9(11)e112747(2014)

The multiple functions of collagen XVIII in development and disease.
Seppinen L, Pihlajaniemi T
Matrix biology : journal of the International Society for Matrix Biology30(2)83-92(2011 Mar)

Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteases.
Heljasvaara R, Nyberg P, Luostarinen J, Parikka M, Heikkilä P, Rehn M, Sorsa T, Salo T, Pihlajaniemi T
Experimental cell research307(2)292-304(2005 Jul)

Characterization of the human type XVIII collagen gene and proteolytic processing and tissue location of the variant containing a frizzled motif.
Elamaa H, Snellman A, Rehn M, Autio-Harmainen H, Pihlajaniemi T
Matrix biology : journal of the International Society for Matrix Biology22(5)427-42(2003 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Blood endostatin wasdddddddddddddddddddddddddf significantly higher in chronic hepatitis C patients with liver fibrosis compared to those without fibrosis.
Title: Noninvasive estimation of liver fibrosis in biopsy-proven hepatitis C virus-infected patients: angiogenic fibrogenic link.

Endostatin is increased in patients with primary sjogren's syndrome patients with interstitial lung disease.
Title: Endostatin is higher and associated with pulmonary involvement in primary Sjögren's syndrome.

Collaterals formation seems to be associated with the activation of pro-inflammatory factors, growth factors and endostatin in chronic heart failure.
Title: Expression of Proinflammatory Factors, Proangiogenic Factors and Endostatin in Patients with Heart Failure and Different Grades of Collateral Circulation Development.

Data show that endostatin levels were elevated in patients with systemic sclerosis (SSc) and mixed connective tissue disease (MCTD).
Title: Associations between circulating endostatin levels and vascular organ damage in systemic sclerosis and mixed connective tissue disease: an observational study.

The homozygous DN and NN genotypes of COL18A1 D104N were associated with the risk of osteosarcoma.
Title: Genetic polymorphisms in COL18A1 influence the development of osteosarcoma.

The following COL18A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL18A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19786 NM_130445.3
Latest version!
Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $384.30
$549.00
OHu19882 NM_030582.3
Latest version!
Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $923.30
$1319.00
OHu72836 NM_130444.2
Latest version!
Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $951.30
$1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19786
Clone ID Related Accession (Same CDS sequence) NM_130445.3
Accession Version NM_130445.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-15
Organism Homo sapiens(Human)
Product collagen alpha-1(XVIII) chain isoform 2 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN389577.1, BC063833.1 and AF018082.1. On Dec 14, 2014 this sequence version replaced NM_130445.2. Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018082.1, BC063833.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_569712.2
CDS95..4105
Misc Feature(1)215..781
Misc Feature(2)<1304..1417
Misc Feature(3)2018..2170
Misc Feature(4)2270..2392
Misc Feature(5)3572..4084
Misc Feature(6)order(3665..3667,3692..3694,3710..3712,3737..3742,3749..3751,3935..3940,3968..3970)
Exon (1)1..105
Exon (2)106..200
Exon (3)201..745
Exon (4)746..832
Exon (5)833..892
Exon (6)893..1022
Exon (7)1023..1099
Exon (8)1100..1315
Exon (9)1316..1342
Exon (10)1343..1405
Exon (11)1406..1492
Exon (12)1493..1546
Exon (13)1547..1705
Exon (14)1706..1768
Exon (15)1769..1795
Exon (16)1796..1927
Exon (17)1928..1990
Exon (18)1991..2017
Exon (19)2018..2053
Exon (20)2054..2125
Exon (21)2126..2161
Exon (22)2162..2251
Exon (23)2252..2281
Exon (24)2282..2308
Exon (25)2309..2371
Exon (26)2372..2446
Exon (27)2447..2473
Exon (28)2474..2527
Exon (29)2528..2602
Exon (30)2603..2671
Exon (31)2672..2714
Exon (32)2715..2777
Exon (33)2778..2821
Exon (34)2822..2953
Exon (35)2954..3098
Exon (36)3099..3172
Exon (37)3173..3301
Exon (38)3302..3334
Exon (39)3335..3580
Exon (40)3581..3778
Exon (41)3779..3894
Exon (42)3895..5427
Translation

Target ORF information:

RefSeq Version NM_130445.3
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_130445.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGCGCCGA GGTGCCCCTG GCCATGGCCG CGGCGGCGGC GCCTCCTGGA CGTGCTCGCG 
CCCCTGGTCC TGCTGCTCGG GGTCCGCGCG GCCTCCGCGG AGCCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19882
Clone ID Related Accession (Same CDS sequence) NM_030582.3
Accession Version NM_030582.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-28
Organism Homo sapiens(Human)
Product collagen alpha-1(XVIII) chain isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. This sequence is a reference standard in the RefSeqGene project. On Jul 20, 2006 this sequence version replaced NM_030582.2. Transcript Variant: This variant (1, also known as NCI-493) uses an alternate splice donor in the 5' terminal exon compared to variant 3. The encoded isoform (1) is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_085059.2
CDS22..4572
Misc Feature(1)94..603
Misc Feature(2)682..1248
Misc Feature(3)<1771..1884
Misc Feature(4)2485..2637
Misc Feature(5)2737..2859
Misc Feature(6)4039..4551
Misc Feature(7)order(4132..4134,4159..4161,4177..4179,4204..4209,4216..4218,4402..4407,4435..4437)
Exon (1)1..667
Exon (2)668..1212
Exon (3)1213..1299
Exon (4)1300..1359
Exon (5)1360..1489
Exon (6)1490..1566
Exon (7)1567..1782
Exon (8)1783..1809
Exon (9)1810..1872
Exon (10)1873..1959
Exon (11)1960..2013
Exon (12)2014..2172
Exon (13)2173..2235
Exon (14)2236..2262
Exon (15)2263..2394
Exon (16)2395..2457
Exon (17)2458..2484
Exon (18)2485..2520
Exon (19)2521..2592
Exon (20)2593..2628
Exon (21)2629..2718
Exon (22)2719..2748
Exon (23)2749..2775
Exon (24)2776..2838
Exon (25)2839..2913
Exon (26)2914..2940
Exon (27)2941..2994
Exon (28)2995..3069
Exon (29)3070..3138
Exon (30)3139..3181
Exon (31)3182..3244
Exon (32)3245..3288
Exon (33)3289..3420
Exon (34)3421..3565
Exon (35)3566..3639
Exon (36)3640..3768
Exon (37)3769..3801
Exon (38)3802..4047
Exon (39)4048..4245
Exon (40)4246..4361
Exon (41)4362..5894
Translation

Target ORF information:

RefSeq Version NM_030582.3
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_030582.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGAGCG CATCAGCGAG
GAGGTGGGGC TGCTGCAGCT CCTTGGGGAC CCCCCGCCCC AGCAGGTCAC CCAGACGGAT
GACCCCGACG TCGGGCTGGC CTACGTCTTT GGGCCAGATG CCAACAGTGG CCAAGTGGCC
CGGTACCACT TCCCCAGCCT CTTCTTCCGT GACTTCTCAC TGCTGTTCCA CATCCGGCCA
GCCACAGAGG GCCCAGGGGT GCTGTTCGCC ATCACGGACT CGGCGCAGGC CATGGTCTTG
CTGGGCGTGA AGCTCTCTGG GGTGCAGGAC GGGCACCAGG ACATCTCCCT GCTCTACACA
GAACCAGGTG CAGGCCAGAC CCACACAGCC GCCAGCTTCC GGCTCCCCGC CTTCGTCGGC
CAGTGGACAC ACTTAGCCCT CAGTGTGGCA GGTGGCTTTG TGGCCCTCTA CGTGGACTGT
GAGGAGTTCC AGAGAATGCC GCTTGCTCGG TCCTCACGGG GCCTGGAGCT GGAGCCTGGC
GCCGGGCTCT TCGTGGCTCA GGCGGGGGGA GCGGACCCTG ACAAGTTCCA GGGGGTGATC
GCTGAGCTGA AGGTGCGCAG GGACCCCCAG GTGAGCCCCA TGCACTGCCT GGACGAGGAA
GGCGATGACT CAGATGGGGC ATCCGGAGAC TCTGGCAGCG GGCTCGGGGA CGCCCGGGAG
CTTCTCAGGG AGGAGACGGG CGCGGCCCTA AAACCCAGGC TCCCCGCGCC ACCCCCCGTC
ACCACGCCAC CCTTGGCTGG AGGCAGCAGC ACGGAAGATT CCAGAAGTGA AGAAGTCGAG
GAGCAGACCA CGGTGGCTTC GTTAGGAGCT CAGACACTTC CTGGCTCAGA TTCTGTCTCC
ACGTGGGACG GGAGTGTCCG GACCCCTGGG GGCCGCGTGA AAGAGGGCGG CCTGAAGGGG
CAGAAAGGGG AGCCAGGTGT TCCGGGCCCA CCTGGCCGGG CAGGCCCCCC AGGATCCCCA
TGCCTACCTG GTCCCCCGGG TCTCCCGTGC CCAGTGAGTC CCCTGGGTCC TGCAGGCCCA
GCGTTGCAAA CTGTCCCCGG ACCACAAGGA CCCCCAGGGC CTCCGGGGAG GGACGGCACC
CCTGGAAGGG ACGGCGAGCC GGGCGACCCC GGTGAAGACG GAAAGCCGGG CGACACCGGG
CCACAAGGCT TCCCCGGGAC TCCAGGGGAC GTAGGTCCCA AGGGCGACAA GGGAGACCCT
GGGGTTGGAG AGAGAGGGCC CCCAGGACCC CAAGGGCCTC CAGGGCCCCC AGGACCCTCC
TTCAGACACG ACAAGCTGAC CTTCATTGAC ATGGAGGGAT CTGGCTTCGG GGGCGATCTG
GAGGCCCTGC GGGGTCCTCG AGGCTTCCCT GGACCTCCCG GACCCCCCGG TGTCCCAGGC
CTGCCCGGCG AGCCAGGCCG CTTTGGGGTG AACAGCTCCG ACGTCCCAGG ACCCGCCGGC
CTTCCTGGTG TGCCTGGGCG CGAGGGTCCC CCCGGGTTTC CTGGCCTCCC GGGACCCCCA
GGCCCTCCGG GAAGAGAGGG GCCCCCAGGA AGGACTGGGC AGAAAGGCAG CCTGGGTGAA
GCAGGCGCCC CAGGACATAA GGGGAGCAAG GGAGCCCCCG GTCCTGCTGG TGCTCGTGGG
GAGAGCGGCC TGGCAGGAGC CCCCGGACCT GCTGGACCAC CAGGCCCCCC TGGGCCCCCT
GGGCCCCCAG GACCAGGACT CCCCGCTGGA TTTGATGACA TGGAAGGCTC CGGGGGGCCC
TTCTGGTCAA CAGCCCGAAG CGCTGATGGG CCACAGGGAC CTCCCGGCCT GCCGGGACTT
AAGGGGGATC CTGGCGTGCC TGGGCTGCCG GGGGCGAAGG GAGAAGTTGG AGCAGATGGA
GTCCCCGGGT TCCCCGGCCT CCCTGGCAGA GAGGGCATTG CTGGGCCCCA GGGGCCAAAG
GGAGACAGAG GCAGCCGGGG AGAAAAGGGA GATCCAGGGA AGGACGGAGT CGGGCAGCCG
GGCCTCCCTG GCCCCCCCGG ACCCCCGGGA CCTGTGGTCT ACGTGTCGGA GCAGGACGGA
TCCGTCCTGA GCGTGCCGGG ACCTGAGGGC CGGCCGGGTT TCGCAGGCTT TCCCGGACCT
GCAGGACCCA AGGGCAACCT GGGCTCTAAG GGCGAACGAG GCTCCCCGGG ACCCAAGGGT
GAGAAGGGTG AACCGGGCAG CATCTTCAGC CCCGACGGCG GTGCCCTGGG CCCTGCCCAG
AAAGGAGCCA AGGGAGAGCC GGGCTTCCGA GGACCCCCGG GTCCATACGG ACGGCCGGGG
TACAAGGGAG AGATTGGCTT TCCTGGACGG CCGGGTCGCC CCGGGATGAA CGGATTGAAA
GGAGAGAAAG GGGAGCCGGG AGATGCCAGC CTTGGATTTG GCATGAGGGG AATGCCCGGC
CCCCCAGGAC CTCCAGGGCC CCCAGGCCCT CCAGGGACTC CTGTTTACGA CAGCAATGTG
TTTGCTGAGT CCAGCCGCCC CGGGCCTCCA GGATTGCCAG GGAATCAGGG CCCTCCAGGA
CCCAAGGGCG CCAAAGGAGA AGTGGGCCCC CCCGGACCAC CAGGGCAGTT TCCGTTTGAC
TTTCTTCAGT TGGAGGCTGA AATGAAGGGG GAGAAGGGAG ACCGAGGTGA TGCAGGACAG
AAAGGCGAAA GGGGGGAGCC CGGGGGCGGC GGTTTCTTCG GCTCCAGCCT GCCCGGCCCC
CCCGGCCCCC CAGGCCCACG TGGCTACCCT GGGATTCCAG GTCCCAAGGG AGAGAGCATC
CGGGGCCAGC CCGGCCCACC TGGACCTCAG GGACCCCCCG GCATCGGCTA CGAGGGGCGC
CAGGGCCCTC CCGGCCCCCC AGGCCCCCCA GGGCCCCCTT CATTTCCTGG CCCTCACAGG
CAGACTATCA GCGTTCCCGG CCCTCCGGGC CCCCCTGGGC CCCCTGGGCC CCCTGGAACC
ATGGGCGCCT CCTCAGGGGT GAGGCTCTGG GCTACACGCC AGGCCATGCT GGGCCAGGTG
CACGAGGTTC CCGAGGGCTG GCTCATCTTC GTGGCCGAGC AGGAGGAGCT CTACGTCCGC
GTGCAGAACG GGTTCCGGAA GGTCCAGCTG GAGGCCCGGA CACCACTCCC ACGAGGGACG
GACAATGAAG TGGCCGCCTT GCAGCCCCCC GTGGTGCAGC TGCACGACAG CAACCCCTAC
CCGCGGCGGG AGCACCCCCA CCCCACCGCG CGGCCCTGGC GGGCAGATGA CATCCTGGCC
AGCCCCCCTC GCCTGCCCGA GCCCCAGCCC TACCCCGGAG CCCCGCACCA CAGCTCCTAC
GTGCACCTGC GGCCGGCGCG ACCCACAAGC CCACCCGCCC ACAGCCACCG CGACTTCCAG
CCGGTGCTCC ACCTGGTTGC GCTCAACAGC CCCCTGTCAG GCGGCATGCG GGGCATCCGC
GGGGCCGACT TCCAGTGCTT CCAGCAGGCG CGGGCCGTGG GGCTGGCGGG CACCTTCCGC
GCCTTCCTGT CCTCGCGCCT GCAGGACCTG TACAGCATCG TGCGCCGTGC CGACCGCGCA
GCCGTGCCCA TCGTCAACCT CAAGGACGAG CTGCTGTTTC CCAGCTGGGA GGCTCTGTTC
TCAGGCTCTG AGGGTCCGCT GAAGCCCGGG GCACGCATCT TCTCCTTTGA CGGCAAGGAC
GTCCTGAGGC ACCCCACCTG GCCCCAGAAG AGCGTGTGGC ATGGCTCGGA CCCCAACGGG
CGCAGGCTGA CCGAGAGCTA CTGTGAGACG TGGCGGACGG AGGCTCCCTC GGCCACGGGC
CAGGCCTCCT CGCTGCTGGG GGGCAGGCTC CTGGGGCAGA GTGCCGCGAG CTGCCATCAC
GCCTACATCG TGCTCTGCAT TGAGAACAGC TTCATGACTG CCTCCAAGTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu72836
Clone ID Related Accession (Same CDS sequence) NM_130444.2
Accession Version NM_130444.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-01-04
Organism Homo sapiens(Human)
Product collagen alpha-1(XVIII) chain isoform 3 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX322561.1, BC063833.1 and AF018082.1. On Sep 25, 2008 this sequence version replaced NM_130444.1. Transcript Variant: This variant (3, also known as NCI-728) represents the longest transcript and encodes the longest isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 14614989 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_569711.2
CDS22..5277
Exon (1)1..1372
Translation

Target ORF information:

RefSeq Version NM_130444.2
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type XVIII alpha 1 chain (COL18A1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_130444.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
ATGGCTCCCT ACCCCTGTGG CTGCCACATC CTGCTGCTGC TCTTCTGCTG CCTGGCGGCT 
GCCCGGGCCA ACCTGCTGAA CCTGAACTGG CTTTGGTTCA ATAATGAGGA CACCAGCCAT
GCAGCTACCA CGATCCCTGA GCCCCAGGGG CCCCTGCCTG TGCAGCCCAC AGCAGATACC
ACCACACACG TGACCCCCCG GAATGGTTCC ACAGAGCCAG CGACAGCCCC TGGCAGCCCT
GAGCCACCCT CAGAGCTGCT GGAAGATGGC CAGGACACCC CCACTTCTGC CGAGAGCCCG
GACGCGCCAG AGGAGAACAT TGCCGGTGTC GGAGCCGAGA TCCTGAACGT GGCCAAAGGC
ATCCGGAGCT TCGTCCAGCT GTGGAATGAC ACTGTCCCCA CTGAGAGCTT GGCCAGGGCG
GAAACCCTGG TCCTGGAGAC TCCTGTGGGC CCCCTTGCCC TCGCTGGGCC TTCCAGCACC
CCCCAGGAGA ATGGGACCAC TCTCTGGCCC AGCCGTGGCA TTCCTAGCTC TCCGGGCGCC
CACACAACCG AGGCTGGCAC CTTGCCTGCA CCCACCCCAT CGCCTCCCTC CCTGGGCAGG
CCCTGGGCAC CACTCACGGG GCCCTCAGTG CCACCACCAT CTTCAGGTAG AGCTTCTCTC
TCCTCCTTGC TGGGCGGGGC CCCTCCCTGG GGAAGCCTGC AGGACCCAGA CAGCCAAGGA
CTCTCGCCCG CCGCAGCCGC TCCCAGCCAG CAGCTCCAAC GCCCTGACGT CCGCCTGCGC
ACGCCACTTC TGCACCCCCT GGTGATGGGC TCCCTGGGCA AGCACGCGGC CCCCTCCGCC
TTCTCCTCTG GGCTCCCGGG CGCACTGTCT CAGGTCGCAG TCACCACTTT AACCAGGGAC
AGCGGTGCTT GGGTCTCCCA CGTGGCTAAC TCTGTGGGGC CGGGTCTTGC TAATAACTCT
GCCCTGCTCG GGGCTGACCC CGAGGCCCCC GCCGGTCGCT GCCTGCCCCT GCCACCCTCC
CTGCCAGTCT GCGGCCACCT GGGCATCTCA CGCTTCTGGC TGCCCAACCA CCTCCACCAC
GAGAGCGGCG AGCAGGTGCG GGCCGGGGCA CGGGCGTGGG GGGGCCTGCT GCAGACGCAC
TGCCACCCCT TCCTCGCCTG GTTCTTCTGC CTGCTGCTGG TCCCCCCATG CGGCAGCGTC
CCGCCGCCCG CCCCGCCACC CTGCTGCCAG TTCTGCGAGG CCCTGCAGGA TGCGTGTTGG
AGCCGCCTGG GCGGGGGCCG GCTGCCCGTC GCCTGTGCCT CGCTCCCGAC CCAGGAGGAT
GGGTACTGTG TGCTCATTGG GCCGGCTGCA GAGCGCATCA GCGAGGAGGT GGGGCTGCTG
CAGCTCCTTG GGGACCCCCC GCCCCAGCAG GTCACCCAGA CGGATGACCC CGACGTCGGG
CTGGCCTACG TCTTTGGGCC AGATGCCAAC AGTGGCCAAG TGGCCCGGTA CCACTTCCCC
AGCCTCTTCT TCCGTGACTT CTCACTGCTG TTCCACATCC GGCCAGCCAC AGAGGGCCCA
GGGGTGCTGT TCGCCATCAC GGACTCGGCG CAGGCCATGG TCTTGCTGGG CGTGAAGCTC
TCTGGGGTGC AGGACGGGCA CCAGGACATC TCCCTGCTCT ACACAGAACC AGGTGCAGGC
CAGACCCACA CAGCCGCCAG CTTCCGGCTC CCCGCCTTCG TCGGCCAGTG GACACACTTA
GCCCTCAGTG TGGCAGGTGG CTTTGTGGCC CTCTACGTGG ACTGTGAGGA GTTCCAGAGA
ATGCCGCTTG CTCGGTCCTC ACGGGGCCTG GAGCTGGAGC CTGGCGCCGG GCTCTTCGTG
GCTCAGGCGG GGGGAGCGGA CCCTGACAAG TTCCAGGGGG TGATCGCTGA GCTGAAGGTG
CGCAGGGACC CCCAGGTGAG CCCCATGCAC TGCCTGGACG AGGAAGGCGA TGACTCAGAT
GGGGCATCCG GAGACTCTGG CAGCGGGCTC GGGGACGCCC GGGAGCTTCT CAGGGAGGAG
ACGGGCGCGG CCCTAAAACC CAGGCTCCCC GCGCCACCCC CCGTCACCAC GCCACCCTTG
GCTGGAGGCA GCAGCACGGA AGATTCCAGA AGTGAAGAAG TCGAGGAGCA GACCACGGTG
GCTTCGTTAG GAGCTCAGAC ACTTCCTGGC TCAGATTCTG TCTCCACGTG GGACGGGAGT
GTCCGGACCC CTGGGGGCCG CGTGAAAGAG GGCGGCCTGA AGGGGCAGAA AGGGGAGCCA
GGTGTTCCGG GCCCACCTGG CCGGGCAGGC CCCCCAGGAT CCCCATGCCT ACCTGGTCCC
CCGGGTCTCC CGTGCCCAGT GAGTCCCCTG GGTCCTGCAG GCCCAGCGTT GCAAACTGTC
CCCGGACCAC AAGGACCCCC AGGGCCTCCG GGGAGGGACG GCACCCCTGG AAGGGACGGC
GAGCCGGGCG ACCCCGGTGA AGACGGAAAG CCGGGCGACA CCGGGCCACA AGGCTTCCCC
GGGACTCCAG GGGACGTAGG TCCCAAGGGC GACAAGGGAG ACCCTGGGGT TGGAGAGAGA
GGGCCCCCAG GACCCCAAGG GCCTCCAGGG CCCCCAGGAC CCTCCTTCAG ACACGACAAG
CTGACCTTCA TTGACATGGA GGGATCTGGC TTCGGGGGCG ATCTGGAGGC CCTGCGGGGT
CCTCGAGGCT TCCCTGGACC TCCCGGACCC CCCGGTGTCC CAGGCCTGCC CGGCGAGCCA
GGCCGCTTTG GGGTGAACAG CTCCGACGTC CCAGGACCCG CCGGCCTTCC TGGTGTGCCT
GGGCGCGAGG GTCCCCCCGG GTTTCCTGGC CTCCCGGGAC CCCCAGGCCC TCCGGGAAGA
GAGGGGCCCC CAGGAAGGAC TGGGCAGAAA GGCAGCCTGG GTGAAGCAGG CGCCCCAGGA
CATAAGGGGA GCAAGGGAGC CCCCGGTCCT GCTGGTGCTC GTGGGGAGAG CGGCCTGGCA
GGAGCCCCCG GACCTGCTGG ACCACCAGGC CCCCCTGGGC CCCCTGGGCC CCCAGGACCA
GGACTCCCCG CTGGATTTGA TGACATGGAA GGCTCCGGGG GGCCCTTCTG GTCAACAGCC
CGAAGCGCTG ATGGGCCACA GGGACCTCCC GGCCTGCCGG GACTTAAGGG GGATCCTGGC
GTGCCTGGGC TGCCGGGGGC GAAGGGAGAA GTTGGAGCAG ATGGAGTCCC CGGGTTCCCC
GGCCTCCCTG GCAGAGAGGG CATTGCTGGG CCCCAGGGGC CAAAGGGAGA CAGAGGCAGC
CGGGGAGAAA AGGGAGATCC AGGGAAGGAC GGAGTCGGGC AGCCGGGCCT CCCTGGCCCC
CCCGGACCCC CGGGACCTGT GGTCTACGTG TCGGAGCAGG ACGGATCCGT CCTGAGCGTG
CCGGGACCTG AGGGCCGGCC GGGTTTCGCA GGCTTTCCCG GACCTGCAGG ACCCAAGGGC
AACCTGGGCT CTAAGGGCGA ACGAGGCTCC CCGGGACCCA AGGGTGAGAA GGGTGAACCG
GGCAGCATCT TCAGCCCCGA CGGCGGTGCC CTGGGCCCTG CCCAGAAAGG AGCCAAGGGA
GAGCCGGGCT TCCGAGGACC CCCGGGTCCA TACGGACGGC CGGGGTACAA GGGAGAGATT
GGCTTTCCTG GACGGCCGGG TCGCCCCGGG ATGAACGGAT TGAAAGGAGA GAAAGGGGAG
CCGGGAGATG CCAGCCTTGG ATTTGGCATG AGGGGAATGC CCGGCCCCCC AGGACCTCCA
GGGCCCCCAG GCCCTCCAGG GACTCCTGTT TACGACAGCA ATGTGTTTGC TGAGTCCAGC
CGCCCCGGGC CTCCAGGATT GCCAGGGAAT CAGGGCCCTC CAGGACCCAA GGGCGCCAAA
GGAGAAGTGG GCCCCCCCGG ACCACCAGGG CAGTTTCCGT TTGACTTTCT TCAGTTGGAG
GCTGAAATGA AGGGGGAGAA GGGAGACCGA GGTGATGCAG GACAGAAAGG CGAAAGGGGG
GAGCCCGGGG GCGGCGGTTT CTTCGGCTCC AGCCTGCCCG GCCCCCCCGG CCCCCCAGGC
CCACGTGGCT ACCCTGGGAT TCCAGGTCCC AAGGGAGAGA GCATCCGGGG CCAGCCCGGC
CCACCTGGAC CTCAGGGACC CCCCGGCATC GGCTACGAGG GGCGCCAGGG CCCTCCCGGC
CCCCCAGGCC CCCCAGGGCC CCCTTCATTT CCTGGCCCTC ACAGGCAGAC TATCAGCGTT
CCCGGCCCTC CGGGCCCCCC TGGGCCCCCT GGGCCCCCTG GAACCATGGG CGCCTCCTCA
GGGGTGAGGC TCTGGGCTAC ACGCCAGGCC ATGCTGGGCC AGGTGCACGA GGTTCCCGAG
GGCTGGCTCA TCTTCGTGGC CGAGCAGGAG GAGCTCTACG TCCGCGTGCA GAACGGGTTC
CGGAAGGTCC AGCTGGAGGC CCGGACACCA CTCCCACGAG GGACGGACAA TGAAGTGGCC
GCCTTGCAGC CCCCCGTGGT GCAGCTGCAC GACAGCAACC CCTACCCGCG GCGGGAGCAC
CCCCACCCCA CCGCGCGGCC CTGGCGGGCA GATGACATCC TGGCCAGCCC CCCTCGCCTG
CCCGAGCCCC AGCCCTACCC CGGAGCCCCG CACCACAGCT CCTACGTGCA CCTGCGGCCG
GCGCGACCCA CAAGCCCACC CGCCCACAGC CACCGCGACT TCCAGCCGGT GCTCCACCTG
GTTGCGCTCA ACAGCCCCCT GTCAGGCGGC ATGCGGGGCA TCCGCGGGGC CGACTTCCAG
TGCTTCCAGC AGGCGCGGGC CGTGGGGCTG GCGGGCACCT TCCGCGCCTT CCTGTCCTCG
CGCCTGCAGG ACCTGTACAG CATCGTGCGC CGTGCCGACC GCGCAGCCGT GCCCATCGTC
AACCTCAAGG ACGAGCTGCT GTTTCCCAGC TGGGAGGCTC TGTTCTCAGG CTCTGAGGGT
CCGCTGAAGC CCGGGGCACG CATCTTCTCC TTTGACGGCA AGGACGTCCT GAGGCACCCC
ACCTGGCCCC AGAAGAGCGT GTGGCATGGC TCGGACCCCA ACGGGCGCAG GCTGACCGAG
AGCTACTGTG AGACGTGGCG GACGGAGGCT CCCTCGGCCA CGGGCCAGGC CTCCTCGCTG
CTGGGGGGCA GGCTCCTGGG GCAGAGTGCC GCGAGCTGCC ATCACGCCTA CATCGTGCTC
TGCATTGAGA ACAGCTTCAT GACTGCCTCC AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Endostatin is higher and associated with pulmonary involvement in primary Sjögren's syndrome.
Clinical and experimental rheumatology34(4)690-3(2016 Jul-Aug)
Palm Ø,Ueland T,Garen T,Michelsen AE,Reiseter S,Aukrust P,Aaløkken TM,Molberg Ø


book

Homozygosity mapping and whole exome sequencing reveal a novel homozygous COL18A1 mutation causing Knobloch syndrome.
PloS one9(11)e112747(2014)
Haghighi A,Tiwari A,Piri N,Nürnberg G,Saleh-Gohari N,Haghighi A,Neidhardt J,Nürnberg P,Berger W


book

The multiple functions of collagen XVIII in development and disease.
Matrix biology : journal of the International Society for Matrix Biology30(2)83-92(2011 Mar)
Seppinen L,Pihlajaniemi T


book

Generation of biologically active endostatin fragments from human collagen XVIII by distinct matrix metalloproteases.
Experimental cell research307(2)292-304(2005 Jul)
Heljasvaara R,Nyberg P,Luostarinen J,Parikka M,Heikkilä P,Rehn M,Sorsa T,Salo T,Pihlajaniemi T


book

Characterization of the human type XVIII collagen gene and proteolytic processing and tissue location of the variant containing a frizzled motif.
Matrix biology : journal of the International Society for Matrix Biology22(5)427-42(2003 Sep)
Elamaa H,Snellman A,Rehn M,Autio-Harmainen H,Pihlajaniemi T


 
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