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CAMK2A cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CAMK2A
Entrez Gene ID 815
Full Name calcium/calmodulin dependent protein kinase II alpha
Synonyms CAMKA
General protein information
Preferred Names

calcium/calmodulin dependent protein kinase II alpha

Names

calcium/calmodulin-dependent protein kinase type II subunit alpha
CaM kinase II alpha subunit
CaM-kinase II alpha chain
CaMK-II alpha subunit
CaMKIINalpha
caM kinase II subunit alpha
caMK-II subunit alpha
calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
calcium/calmodulin-dependent protein kinase II alpha-B subunit
calcium/calmodulin-dependent protein kinase type II alpha chain

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q32

Summary The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008].
Disorder MIM:

114078


mRNA and Protein(s)

mRNA Protein Name
NM_171825.2 NP_741960.1 calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
XM_017009898.1 XP_016865387.1 calcium/calmodulin-dependent protein kinase type II subunit alpha isoform X1
NM_015981.3 NP_057065.2 calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 1



Mus musculus (house mouse) Camk2a NP_803126.1
Gallus gallus (chicken) CAMK2A NP_989626.1
Danio rerio (zebrafish) camk2a NP_001017741.2
Oryza sativa (rice) Os12g0133500 NP_001176777.1
Pan troglodytes (chimpanzee) CAMK2A XP_001165329.2
Xenopus tropicalis (tropical clawed frog) camk2a NP_001093737.1
Homo sapiens (human) CAMK2A NP_057065.2
Canis lupus familiaris (dog) CAMK2A XP_005619357.1
Bos taurus (cattle) CAMK2A NP_001069406.1
Rattus norvegicus (Norway rat) Camk2a NP_037052.1
Oryza sativa (rice) Os11g0136600 NP_001065691.1


Related articles in PubMed

CaMKII Phosphorylation of Na(V)1.5: Novel in Vitro Sites Identified by Mass Spectrometry and Reduced S516 Phosphorylation in Human Heart Failure.
Herren AW, Weber DM, Rigor RR, Margulies KB, Phinney BS, Bers DM
Journal of proteome research14(5)2298-311(2015 May)

Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.
So J, Pasculescu A, Dai AY, Williton K, James A, Nguyen V, Creixell P, Schoof EM, Sinclair J, Barrios-Rodiles M, Gu J, Krizus A, Williams R, Olhovsky M, Dennis JW, Wrana JL, Linding R, Jorgensen C, Pawson T, Colwill K
Science signaling8(371)rs3(2015 Apr)

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
Chen TC, Lin KT, Chen CH, Lee SA, Lee PY, Liu YW, Kuo YL, Wang FS, Lai JM, Huang CY
Journal of proteome research13(12)5339-46(2014 Dec)

αCaMKII controls the establishment of cocaine's reinforcing effects in mice and humans.
Easton AC, Lourdusamy A, Havranek M, Mizuno K, Solati J, Golub Y, Clarke TK, Vallada H, Laranjeira R, Desrivières S, Moll GH, Mössner R, Kornhuber J, Schumann G, Giese KP, Fernandes C, Quednow BB, Müller CP
Translational psychiatry4e457(2014 Oct)

Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII).
Coultrap SJ, Bayer KU
The Journal of biological chemistry289(28)19458-65(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrate that this ASD-linked de novo CAMK2A mutation disrupts multiple CaMKII functions, induces synaptic deficits, and causes ASD-related behavioral alterations.
Title: A Novel Human

CaMKII phosphorylates SCN5A in vitro on 23 novel serine sites as was identified by mass spectrometry; reduced S516 phosphorylation has been found in human heart failure.
Title: CaMKII Phosphorylation of Na(V)1.5: Novel in Vitro Sites Identified by Mass Spectrometry and Reduced S516 Phosphorylation in Human Heart Failure.

Ca2+/calmodulin-dependent protein kinase-II (CaMKII) has a key role in the plasticity of glutamatergic synapses of the brain.
Title: αCaMKII controls the establishment of cocaine's reinforcing effects in mice and humans.

a novel regulation of CaMKII by another second messenger system and indicate its involvement in excitotoxic neuronal cell death.
Title: Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII).

CaMKII-mediated recruitment and upregulation of CYLD is expected to remove K63-linked polyubiquitins and facilitate proteasomal degradation at the postsynaptic density.
Title: CaMKII mediates recruitment and activation of the deubiquitinase CYLD at the postsynaptic density.

The following CAMK2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAMK2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu15670 NM_015981.3
Latest version!
Homo sapiens calcium/calmodulin dependent protein kinase II alpha (CAMK2A), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $286.30
$409.00
OHu15931 NM_171825.2
Latest version!
Homo sapiens calcium/calmodulin dependent protein kinase II alpha (CAMK2A), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu96104 XM_017009898.1
Latest version!
Homo sapiens calcium/calmodulin dependent protein kinase II alpha (CAMK2A), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15670
Clone ID Related Accession (Same CDS sequence) NM_015981.3
Accession Version NM_015981.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-05
Organism Homo sapiens(Human)
Product calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289776.1, BF951952.1, AC011372.7 and BC040457.1. This sequence is a reference standard in the RefSeqGene project. On Nov 13, 2008 this sequence version replaced NM_015981.2. Transcript Variant: This variant (1) represents the longer transcript, and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289776.1, SRR1803614.30654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCACCA TCACCTGCAC CCGCTTCACG GAAGAGTACC AGCTCTTCGA GGAATTGGGC 
AAGGGAGCCT TCTCGGTGGT GCGAAGGTGT GTGAAGGTGC TGGCTGGCCA GGAGTATGCT
GCCAAGATCA TCAACACAAA GAAGCTGTCA GCCAGAGACC ATCAGAAGCT GGAGCGTGAA
GCCCGCATCT GCCGCCTGCT GAAGCACCCC AACATCGTCC GACTACATGA CAGCATCTCA
GAGGAGGGAC ACCACTACCT GATCTTCGAC CTGGTCACTG GTGGGGAACT GTTTGAAGAT
ATCGTGGCCC GGGAGTATTA CAGTGAGGCG GATGCCAGTC ACTGTATCCA GCAGATCCTG
GAGGCTGTGC TGCACTGCCA CCAGATGGGG GTGGTGCACC GGGACCTGAA GCCTGAGAAT
CTGTTGCTGG CCTCCAAGCT CAAGGGTGCC GCAGTGAAGC TGGCAGACTT TGGCCTGGCC
ATAGAGGTGG AGGGGGAGCA GCAGGCATGG TTTGGGTTTG CAGGGACTCC TGGATATCTC
TCCCCAGAAG TGCTGCGGAA GGACCCGTAC GGGAAGCCTG TGGACCTGTG GGCTTGTGGG
GTCATCCTGT ACATCCTGCT GGTTGGGTAC CCCCCGTTCT GGGATGAGGA CCAGCACCGC
CTGTACCAGC AGATCAAAGC CGGCGCCTAT GATTTCCCAT CGCCGGAATG GGACACTGTC
ACCCCGGAAG CCAAGGATCT GATCAATAAG ATGCTGACCA TTAACCCATC CAAACGCATC
ACAGCTGCCG AAGCCCTTAA GCACCCCTGG ATCTCGCACC GCTCCACCGT GGCATCCTGC
ATGCACAGAC AGGAGACCGT GGACTGCCTG AAGAAGTTCA ATGCCAGGAG GAAACTGAAG
GGAGCCATTC TCACCACGAT GCTGGCCACC AGGAACTTCT CCGGAGGGAA GAGTGGGGGA
AACAAGAAGA GCGATGGTGT GAAGAAAAGA AAGTCCAGTT CCAGCGTTCA GTTAATGGAA
TCCTCAGAGA GCACCAACAC CACCATCGAG GATGAAGACA CCAAAGTGCG GAAACAGGAA
ATTATAAAAG TGACAGAGCA GCTGATTGAA GCCATAAGCA ATGGAGATTT TGAGTCCTAC
ACGAAGATGT GCGACCCTGG CATGACAGCC TTCGAACCTG AGGCCCTGGG GAACCTGGTT
GAGGGCCTGG ACTTCCATCG ATTCTATTTT GAAAACCTGT GGTCCCGGAA CAGCAAGCCC
GTGCACACCA CCATCCTGAA TCCCCACATC CACCTGATGG GCGACGAGTC AGCCTGCATC
GCCTACATCC GCATCACGCA GTACCTGGAC GCTGGCGGCA TCCCACGCAC CGCCCAGTCG
GAGGAGACCC GTGTCTGGCA CCGCCGGGAT GGCAAATGGC AGATCGTCCA CTTCCACAGA
TCTGGGGCGC CCTCCGTCCT GCCCCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_057065.2
CDS216..1685
Misc Feature(1)45..47
Misc Feature(2)246..1121
Misc Feature(3)252..1028
Misc Feature(4)order(270..284,294..296,333..335,339..341,432..434,480..491,501..503,507..509,615..620,624..626,630..635,639..641,678..683,690..692,735..749,753..755)
Misc Feature(5)order(270..281,294..296,333..335,339..341,432..434,480..491,501..503,630..635,639..641,678..683)
Misc Feature(6)order(282..284,501..503,507..509,615..620,624..626,630..632,690..692,735..749,753..755)
Misc Feature(7)order(678..710,717..749)
Misc Feature(8)1071..1073
Misc Feature(9)1083..1115
Misc Feature(10)order(1092..1094,1098..1121)
Misc Feature(11)1128..1130
Misc Feature(12)1284..1667
Exon (1)1..277
Exon (2)278..372
Exon (3)373..432
Exon (4)433..487
Exon (5)488..553
Exon (6)554..626
Exon (7)627..729
Exon (8)730..813
Exon (9)814..908
Exon (10)909..1031
Exon (11)1032..1115
Exon (12)1116..1158
Exon (13)1159..1199
Exon (14)1200..1232
Exon (15)1233..1281
Exon (16)1282..1357
Exon (17)1358..1452
Exon (18)1453..1681
Exon (19)1682..4904
Translation

Target ORF information:

RefSeq Version NM_015981.3
Organism Homo sapiens(Human)
Definition Homo sapiens calcium/calmodulin dependent protein kinase II alpha (CAMK2A), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_015981.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCACCA TCACCTGCAC CCGCTTCACG GAAGAGTACC AGCTCTTCGA GGAATTGGGC 
AAGGGAGCCT TCTCGGTGGT GCGAAGGTGT GTGAAGGTGC TGGCTGGCCA GGAGTATGCT
GCCAAGATCA TCAACACAAA GAAGCTGTCA GCCAGAGACC ATCAGAAGCT GGAGCGTGAA
GCCCGCATCT GCCGCCTGCT GAAGCACCCC AACATCGTCC GACTACATGA CAGCATCTCA
GAGGAGGGAC ACCACTACCT GATCTTCGAC CTGGTCACTG GTGGGGAACT GTTTGAAGAT
ATCGTGGCCC GGGAGTATTA CAGTGAGGCG GATGCCAGTC ACTGTATCCA GCAGATCCTG
GAGGCTGTGC TGCACTGCCA CCAGATGGGG GTGGTGCACC GGGACCTGAA GCCTGAGAAT
CTGTTGCTGG CCTCCAAGCT CAAGGGTGCC GCAGTGAAGC TGGCAGACTT TGGCCTGGCC
ATAGAGGTGG AGGGGGAGCA GCAGGCATGG TTTGGGTTTG CAGGGACTCC TGGATATCTC
TCCCCAGAAG TGCTGCGGAA GGACCCGTAC GGGAAGCCTG TGGACCTGTG GGCTTGTGGG
GTCATCCTGT ACATCCTGCT GGTTGGGTAC CCCCCGTTCT GGGATGAGGA CCAGCACCGC
CTGTACCAGC AGATCAAAGC CGGCGCCTAT GATTTCCCAT CGCCGGAATG GGACACTGTC
ACCCCGGAAG CCAAGGATCT GATCAATAAG ATGCTGACCA TTAACCCATC CAAACGCATC
ACAGCTGCCG AAGCCCTTAA GCACCCCTGG ATCTCGCACC GCTCCACCGT GGCATCCTGC
ATGCACAGAC AGGAGACCGT GGACTGCCTG AAGAAGTTCA ATGCCAGGAG GAAACTGAAG
GGAGCCATTC TCACCACGAT GCTGGCCACC AGGAACTTCT CCGGAGGGAA GAGTGGGGGA
AACAAGAAGA GCGATGGTGT GAAGAAAAGA AAGTCCAGTT CCAGCGTTCA GTTAATGGAA
TCCTCAGAGA GCACCAACAC CACCATCGAG GATGAAGACA CCAAAGTGCG GAAACAGGAA
ATTATAAAAG TGACAGAGCA GCTGATTGAA GCCATAAGCA ATGGAGATTT TGAGTCCTAC
ACGAAGATGT GCGACCCTGG CATGACAGCC TTCGAACCTG AGGCCCTGGG GAACCTGGTT
GAGGGCCTGG ACTTCCATCG ATTCTATTTT GAAAACCTGT GGTCCCGGAA CAGCAAGCCC
GTGCACACCA CCATCCTGAA TCCCCACATC CACCTGATGG GCGACGAGTC AGCCTGCATC
GCCTACATCC GCATCACGCA GTACCTGGAC GCTGGCGGCA TCCCACGCAC CGCCCAGTCG
GAGGAGACCC GTGTCTGGCA CCGCCGGGAT GGCAAATGGC AGATCGTCCA CTTCCACAGA
TCTGGGGCGC CCTCCGTCCT GCCCCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15931
Clone ID Related Accession (Same CDS sequence) NM_171825.2
Accession Version NM_171825.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-05
Organism Homo sapiens(Human)
Product calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK289776.1, AB023185.1, BF951952.1, AC011372.7 and BC040457.1. On Nov 13, 2008 this sequence version replaced NM_171825.1. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter protein (isoform 2), that is missing an internal segment when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040457.1, AB023185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACCA TCACCTGCAC CCGCTTCACG GAAGAGTACC AGCTCTTCGA GGAATTGGGC 
AAGGGAGCCT TCTCGGTGGT GCGAAGGTGT GTGAAGGTGC TGGCTGGCCA GGAGTATGCT
GCCAAGATCA TCAACACAAA GAAGCTGTCA GCCAGAGACC ATCAGAAGCT GGAGCGTGAA
GCCCGCATCT GCCGCCTGCT GAAGCACCCC AACATCGTCC GACTACATGA CAGCATCTCA
GAGGAGGGAC ACCACTACCT GATCTTCGAC CTGGTCACTG GTGGGGAACT GTTTGAAGAT
ATCGTGGCCC GGGAGTATTA CAGTGAGGCG GATGCCAGTC ACTGTATCCA GCAGATCCTG
GAGGCTGTGC TGCACTGCCA CCAGATGGGG GTGGTGCACC GGGACCTGAA GCCTGAGAAT
CTGTTGCTGG CCTCCAAGCT CAAGGGTGCC GCAGTGAAGC TGGCAGACTT TGGCCTGGCC
ATAGAGGTGG AGGGGGAGCA GCAGGCATGG TTTGGGTTTG CAGGGACTCC TGGATATCTC
TCCCCAGAAG TGCTGCGGAA GGACCCGTAC GGGAAGCCTG TGGACCTGTG GGCTTGTGGG
GTCATCCTGT ACATCCTGCT GGTTGGGTAC CCCCCGTTCT GGGATGAGGA CCAGCACCGC
CTGTACCAGC AGATCAAAGC CGGCGCCTAT GATTTCCCAT CGCCGGAATG GGACACTGTC
ACCCCGGAAG CCAAGGATCT GATCAATAAG ATGCTGACCA TTAACCCATC CAAACGCATC
ACAGCTGCCG AAGCCCTTAA GCACCCCTGG ATCTCGCACC GCTCCACCGT GGCATCCTGC
ATGCACAGAC AGGAGACCGT GGACTGCCTG AAGAAGTTCA ATGCCAGGAG GAAACTGAAG
GGAGCCATTC TCACCACGAT GCTGGCCACC AGGAACTTCT CCGGAGGGAA GAGTGGGGGA
AACAAGAAGA GCGATGGTGT GAAGGAATCC TCAGAGAGCA CCAACACCAC CATCGAGGAT
GAAGACACCA AAGTGCGGAA ACAGGAAATT ATAAAAGTGA CAGAGCAGCT GATTGAAGCC
ATAAGCAATG GAGATTTTGA GTCCTACACG AAGATGTGCG ACCCTGGCAT GACAGCCTTC
GAACCTGAGG CCCTGGGGAA CCTGGTTGAG GGCCTGGACT TCCATCGATT CTATTTTGAA
AACCTGTGGT CCCGGAACAG CAAGCCCGTG CACACCACCA TCCTGAATCC CCACATCCAC
CTGATGGGCG ACGAGTCAGC CTGCATCGCC TACATCCGCA TCACGCAGTA CCTGGACGCT
GGCGGCATCC CACGCACCGC CCAGTCGGAG GAGACCCGTG TCTGGCACCG CCGGGATGGC
AAATGGCAGA TCGTCCACTT CCACAGATCT GGGGCGCCCT CCGTCCTGCC CCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_741960.1
CDS216..1652
Misc Feature(1)45..47
Misc Feature(2)246..1121
Misc Feature(3)252..1028
Misc Feature(4)252..254
Misc Feature(5)order(270..284,294..296,333..335,339..341,432..434,480..491,501..503,507..509,615..620,624..626,630..635,639..641,678..683,690..692,735..749,753..755)
Misc Feature(6)order(270..281,294..296,333..335,339..341,432..434,480..491,501..503,630..635,639..641,678..683)
Misc Feature(7)order(282..284,501..503,507..509,615..620,624..626,630..632,690..692,735..749,753..755)
Misc Feature(8)order(678..710,717..749)
Misc Feature(9)984..986
Misc Feature(10)1071..1073
Misc Feature(11)1071..1073
Misc Feature(12)1083..1115
Misc Feature(13)order(1092..1094,1098..1121)
Misc Feature(14)1128..1130
Misc Feature(15)1143..1175
Misc Feature(16)1203..1205
Misc Feature(17)1206..1208
Misc Feature(18)1212..1214
Misc Feature(19)1221..1223
Misc Feature(20)1224..1226
Misc Feature(21)1251..1634
Misc Feature(22)1425..1427
Exon (1)1..277
Exon (2)278..372
Exon (3)373..432
Exon (4)433..487
Exon (5)488..553
Exon (6)554..626
Exon (7)627..729
Exon (8)730..813
Exon (9)814..908
Exon (10)909..1031
Exon (11)1032..1115
Exon (12)1116..1158
Exon (13)1159..1199
Exon (14)1200..1248
Exon (15)1249..1324
Exon (16)1325..1419
Exon (17)1420..1648
Exon (18)1649..4871
Translation

Target ORF information:

RefSeq Version NM_171825.2
Organism Homo sapiens(Human)
Definition Homo sapiens calcium/calmodulin dependent protein kinase II alpha (CAMK2A), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_171825.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACCA TCACCTGCAC CCGCTTCACG GAAGAGTACC AGCTCTTCGA GGAATTGGGC 
AAGGGAGCCT TCTCGGTGGT GCGAAGGTGT GTGAAGGTGC TGGCTGGCCA GGAGTATGCT
GCCAAGATCA TCAACACAAA GAAGCTGTCA GCCAGAGACC ATCAGAAGCT GGAGCGTGAA
GCCCGCATCT GCCGCCTGCT GAAGCACCCC AACATCGTCC GACTACATGA CAGCATCTCA
GAGGAGGGAC ACCACTACCT GATCTTCGAC CTGGTCACTG GTGGGGAACT GTTTGAAGAT
ATCGTGGCCC GGGAGTATTA CAGTGAGGCG GATGCCAGTC ACTGTATCCA GCAGATCCTG
GAGGCTGTGC TGCACTGCCA CCAGATGGGG GTGGTGCACC GGGACCTGAA GCCTGAGAAT
CTGTTGCTGG CCTCCAAGCT CAAGGGTGCC GCAGTGAAGC TGGCAGACTT TGGCCTGGCC
ATAGAGGTGG AGGGGGAGCA GCAGGCATGG TTTGGGTTTG CAGGGACTCC TGGATATCTC
TCCCCAGAAG TGCTGCGGAA GGACCCGTAC GGGAAGCCTG TGGACCTGTG GGCTTGTGGG
GTCATCCTGT ACATCCTGCT GGTTGGGTAC CCCCCGTTCT GGGATGAGGA CCAGCACCGC
CTGTACCAGC AGATCAAAGC CGGCGCCTAT GATTTCCCAT CGCCGGAATG GGACACTGTC
ACCCCGGAAG CCAAGGATCT GATCAATAAG ATGCTGACCA TTAACCCATC CAAACGCATC
ACAGCTGCCG AAGCCCTTAA GCACCCCTGG ATCTCGCACC GCTCCACCGT GGCATCCTGC
ATGCACAGAC AGGAGACCGT GGACTGCCTG AAGAAGTTCA ATGCCAGGAG GAAACTGAAG
GGAGCCATTC TCACCACGAT GCTGGCCACC AGGAACTTCT CCGGAGGGAA GAGTGGGGGA
AACAAGAAGA GCGATGGTGT GAAGGAATCC TCAGAGAGCA CCAACACCAC CATCGAGGAT
GAAGACACCA AAGTGCGGAA ACAGGAAATT ATAAAAGTGA CAGAGCAGCT GATTGAAGCC
ATAAGCAATG GAGATTTTGA GTCCTACACG AAGATGTGCG ACCCTGGCAT GACAGCCTTC
GAACCTGAGG CCCTGGGGAA CCTGGTTGAG GGCCTGGACT TCCATCGATT CTATTTTGAA
AACCTGTGGT CCCGGAACAG CAAGCCCGTG CACACCACCA TCCTGAATCC CCACATCCAC
CTGATGGGCG ACGAGTCAGC CTGCATCGCC TACATCCGCA TCACGCAGTA CCTGGACGCT
GGCGGCATCC CACGCACCGC CCAGTCGGAG GAGACCCGTG TCTGGCACCG CCGGGATGGC
AAATGGCAGA TCGTCCACTT CCACAGATCT GGGGCGCCCT CCGTCCTGCC CCACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu96104
Clone ID Related Accession (Same CDS sequence) XM_017009898.1
Accession Version XM_017009898.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product calcium/calmodulin-dependent protein kinase type II subunit alpha isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACCA TCACCTGCAC CCGCTTCACG GAAGAGTACC AGCTCTTCGA GGAATTGGGC 
AAGGGAGCCT TCTCGGTGGT GCGAAGGTGT GTGAAGGTGC TGGCTGGCCA GGAGTATGCT
GCCAAGATCA TCAACACAAA GAAGCTGTCA GCCAGAGACC ATCAGAAGCT GGAGCGTGAA
GCCCGCATCT GCCGCCTGCT GAAGCACCCC AACATCGTCC GACTACATGA CAGCATCTCA
GAGGAGGGAC ACCACTACCT GATCTTCGAC CTGGTCACTG GTGGGGAACT GTTTGAAGAT
ATCGTGGCCC GGGAGTATTA CAGTGAGGCG GATGCCAGTC ACTGTATCCA GCAGATCCTG
GAGGCTGTGC TGCACTGCCA CCAGATGGGG GTGGTGCACC GGGACCTGAA GCCTGAGAAT
CTGTTGCTGG CCTCCAAGCT CAAGGGTGCC GCAGTGAAGC TGGCAGACTT TGGCCTGGCC
ATAGAGGTGG AGGGGGAGCA GCAGGCATGG TTTGGGTTTG CAGGGACTCC TGGATATCTC
TCCCCAGAAG TGCTGCGGAA GGACCCGTAC GGGAAGCCTG TGGACCTGTG GGCTTGTGGG
GTCATCCTGT ACATCCTGCT GGTTGGGTAC CCCCCGTTCT GGGATGAGGA CCAGCACCGC
CTGTACCAGC AGATCAAAGC CGGCGCCTAT GATTTCCCAT CGCCGGAATG GGACACTGTC
ACCCCGGAAG CCAAGGATCT GATCAATAAG ATGCTGACCA TTAACCCATC CAAACGCATC
ACAGCTGCCG AAGCCCTTAA GCACCCCTGG ATCTCGCACC GCTCCACCGT GGCATCCTGC
ATGCACAGAC AGGAGACCGT GGACTGCCTG AAGAAGTTCA ATGCCAGGAG GAAACTGAAG
GGAGCCATTC TCACCACGAT GCTGGCCACC AGGAACTTCT CCGGAGGGAA GAGTGGGGGA
AACAAGAAGA GCGATGGTGT GAAGGAATCC TCAGAGAGCA CCAACACCAC CATCGAGGAT
GAAGACACCA AAGTGCGGAA ACAGGAAATT ATAAAAGTGA CAGAGCAGCT GATTGAAGCC
ATAAGCAATG GAGATTTTGA GTCCTACACG AAGATGTGCG ACCCTGGCAT GACAGCCTTC
GAACCTGAGG CCCTGGGGAA CCTGGTTGAG GGCCTGGACT TCCATCGATT CTATTTTGAA
AACCTGTGGT CCCGGAACAG CAAGCCCGTG CACACCACCA TCCTGAATCC CCACATCCAC
CTGATGGGCG ACGAGTCAGC CTGCATCGCC TACATCCGCA TCACGCAGTA CCTGGACGCT
GGCGGCATCC CACGCACCGC CCAGTCGGAG GAGACCCGTG TCTGGCACCG CCGGGATGGC
AAATGGCAGA TCGTCCACTT CCACAGATCT GGGGCGCCCT CCGTCCTGCC CCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016865387.1
CDS216..1652
Misc Feature(1)246..1121
Misc Feature(2)252..1028
Misc Feature(3)order(270..284,294..296,333..335,339..341,432..434,480..491,501..503,507..509,615..620,624..626,630..635,639..641,678..683,690..692,735..749,753..755)
Misc Feature(4)order(270..281,294..296,333..335,339..341,432..434,480..491,501..503,630..635,639..641,678..683)
Misc Feature(5)order(282..284,501..503,507..509,615..620,624..626,630..632,690..692,735..749,753..755)
Misc Feature(6)order(678..710,717..749)
Misc Feature(7)order(1092..1094,1098..1121)
Misc Feature(8)1251..1634
Translation

Target ORF information:

RefSeq Version XM_017009898.1
Organism Homo sapiens(Human)
Definition Homo sapiens calcium/calmodulin dependent protein kinase II alpha (CAMK2A), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009898.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCCACCA TCACCTGCAC CCGCTTCACG GAAGAGTACC AGCTCTTCGA GGAATTGGGC 
AAGGGAGCCT TCTCGGTGGT GCGAAGGTGT GTGAAGGTGC TGGCTGGCCA GGAGTATGCT
GCCAAGATCA TCAACACAAA GAAGCTGTCA GCCAGAGACC ATCAGAAGCT GGAGCGTGAA
GCCCGCATCT GCCGCCTGCT GAAGCACCCC AACATCGTCC GACTACATGA CAGCATCTCA
GAGGAGGGAC ACCACTACCT GATCTTCGAC CTGGTCACTG GTGGGGAACT GTTTGAAGAT
ATCGTGGCCC GGGAGTATTA CAGTGAGGCG GATGCCAGTC ACTGTATCCA GCAGATCCTG
GAGGCTGTGC TGCACTGCCA CCAGATGGGG GTGGTGCACC GGGACCTGAA GCCTGAGAAT
CTGTTGCTGG CCTCCAAGCT CAAGGGTGCC GCAGTGAAGC TGGCAGACTT TGGCCTGGCC
ATAGAGGTGG AGGGGGAGCA GCAGGCATGG TTTGGGTTTG CAGGGACTCC TGGATATCTC
TCCCCAGAAG TGCTGCGGAA GGACCCGTAC GGGAAGCCTG TGGACCTGTG GGCTTGTGGG
GTCATCCTGT ACATCCTGCT GGTTGGGTAC CCCCCGTTCT GGGATGAGGA CCAGCACCGC
CTGTACCAGC AGATCAAAGC CGGCGCCTAT GATTTCCCAT CGCCGGAATG GGACACTGTC
ACCCCGGAAG CCAAGGATCT GATCAATAAG ATGCTGACCA TTAACCCATC CAAACGCATC
ACAGCTGCCG AAGCCCTTAA GCACCCCTGG ATCTCGCACC GCTCCACCGT GGCATCCTGC
ATGCACAGAC AGGAGACCGT GGACTGCCTG AAGAAGTTCA ATGCCAGGAG GAAACTGAAG
GGAGCCATTC TCACCACGAT GCTGGCCACC AGGAACTTCT CCGGAGGGAA GAGTGGGGGA
AACAAGAAGA GCGATGGTGT GAAGGAATCC TCAGAGAGCA CCAACACCAC CATCGAGGAT
GAAGACACCA AAGTGCGGAA ACAGGAAATT ATAAAAGTGA CAGAGCAGCT GATTGAAGCC
ATAAGCAATG GAGATTTTGA GTCCTACACG AAGATGTGCG ACCCTGGCAT GACAGCCTTC
GAACCTGAGG CCCTGGGGAA CCTGGTTGAG GGCCTGGACT TCCATCGATT CTATTTTGAA
AACCTGTGGT CCCGGAACAG CAAGCCCGTG CACACCACCA TCCTGAATCC CCACATCCAC
CTGATGGGCG ACGAGTCAGC CTGCATCGCC TACATCCGCA TCACGCAGTA CCTGGACGCT
GGCGGCATCC CACGCACCGC CCAGTCGGAG GAGACCCGTG TCTGGCACCG CCGGGATGGC
AAATGGCAGA TCGTCCACTT CCACAGATCT GGGGCGCCCT CCGTCCTGCC CCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

CaMKII Phosphorylation of Na(V)1.5: Novel in Vitro Sites Identified by Mass Spectrometry and Reduced S516 Phosphorylation in Human Heart Failure.
Journal of proteome research14(5)2298-311(2015 May)
Herren AW,Weber DM,Rigor RR,Margulies KB,Phinney BS,Bers DM


book

Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.
Science signaling8(371)rs3(2015 Apr)
So J,Pasculescu A,Dai AY,Williton K,James A,Nguyen V,Creixell P,Schoof EM,Sinclair J,Barrios-Rodiles M,Gu J,Krizus A,Williams R,Olhovsky M,Dennis JW,Wrana JL,Linding R,Jorgensen C,Pawson T,Colwill K


book

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
Journal of proteome research13(12)5339-46(2014 Dec)
Chen TC,Lin KT,Chen CH,Lee SA,Lee PY,Liu YW,Kuo YL,Wang FS,Lai JM,Huang CY


book

αCaMKII controls the establishment of cocaine's reinforcing effects in mice and humans.
Translational psychiatry4e457(2014 Oct)
Easton AC,Lourdusamy A,Havranek M,Mizuno K,Solati J,Golub Y,Clarke TK,Vallada H,Laranjeira R,Desrivières S,Moll GH,Mössner R,Kornhuber J,Schumann G,Giese KP,Fernandes C,Quednow BB,Müller CP


book

Nitric oxide induces Ca2+-independent activity of the Ca2+/calmodulin-dependent protein kinase II (CaMKII).
The Journal of biological chemistry289(28)19458-65(2014 Jul)
Coultrap SJ,Bayer KU


 
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