×

ADAMTS10 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ADAMTS10
Entrez Gene ID 81794
Full Name ADAM metallopeptidase with thrombospondin type 1 motif 10
Synonyms ADAM-TS10,ADAMTS-10,WMS,WMS1
General protein information
Preferred Names

ADAM metallopeptidase with thrombospondin type 1 motif 10

Names

A disintegrin and metalloproteinase with thrombospondin motifs 10
ADAM-TS 10
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10
zinc metalloendopeptidase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19p13.2

Summary This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:

608990

Disorder Html: Weill-Marchesani syndrome, recessive, 277600 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_017027340.1 XP_016882829.1 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X4
NM_001282352.1 NP_001269281.1 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 2
XM_017027339.1 XP_016882828.1 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X3
XM_017027338.1 XP_016882827.1 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X1
NM_030957.3 NP_112219.3 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein
XM_006722917.3 XP_006722980.1 A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X2



Homo sapiens (human) ADAMTS10 NP_112219.3
Bos taurus (cattle) ADAMTS10 NP_001192679.1
Gallus gallus (chicken) ADAMTS10 XP_001232250.1
Canis lupus familiaris (dog) ADAMTS10 XP_854320.1
Macaca mulatta (rhesus monkey) ADAMTS10 XP_001095217.1
Xenopus tropicalis (tropical clawed frog) adamts10 XP_002938612.2
Pan troglodytes (chimpanzee) ADAMTS10 XP_001159753.1
Mus musculus (house mouse) Adamts10 NP_766207.2
Rattus norvegicus (Norway rat) Adamts10 NP_001258374.1
Danio rerio (zebrafish) adamts10 NP_001116746.1


Related articles in PubMed

Screening ADAMTS10 in dog populations supports Gly661Arg as the glaucoma-causing variant in beagles.
Kuchtey J, Kunkel J, Esson D, Sapienza JS, Ward DA, Plummer CE, Gelatt KN, Kuchtey RW
Investigative ophthalmology & visual science54(3)1881-6(2013 Mar)

SuperTAG methylation-specific digital karyotyping reveals uremia-induced epigenetic dysregulation of atherosclerosis-related genes.
Zawada AM, Rogacev KS, Hummel B, Grün OS, Friedrich A, Rotter B, Winter P, Geisel J, Fliser D, Heine GH
Circulation. Cardiovascular genetics5(6)611-20(2012 Dec)

ADAMTS10 protein interacts with fibrillin-1 and promotes its deposition in extracellular matrix of cultured fibroblasts.
Kutz WE, Wang LW, Bader HL, Majors AK, Iwata K, Traboulsi EI, Sakai LY, Keene DR, Apte SS
The Journal of biological chemistry286(19)17156-67(2011 May)

Homozygous mutations in ADAMTS10 and ADAMTS17 cause lenticular myopia, ectopia lentis, glaucoma, spherophakia, and short stature.
Morales J, Al-Sharif L, Khalil DS, Shinwari JM, Bavi P, Al-Mahrouqi RA, Al-Rajhi A, Alkuraya FS, Meyer BF, Al Tassan N
American journal of human genetics85(5)558-68(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings support the Gly661Arg mutation of ADAMTS10 as the likely cause of POAG in beagles.
Title: Screening ADAMTS10 in dog populations supports Gly661Arg as the glaucoma-causing variant in beagles.

ADAMTS10 participates in microfibril biogenesis rather than in fibrillin-1 turnover
Title: ADAMTS10 protein interacts with fibrillin-1 and promotes its deposition in extracellular matrix of cultured fibroblasts.

Homozygous mutation in ADAMTS10 causes lenticular myopia, ectopia lentis, glaucoma, spherophakia, and short stature.
Title: Homozygous mutations in ADAMTS10 and ADAMTS17 cause lenticular myopia, ectopia lentis, glaucoma, spherophakia, and short stature.

Mutations in ADAMTS10 gene is not responsible for microspherophakia.
Title: Clinical and genetic investigation of isolated microspherophakia in a consanguineous Tunisian family.

infer that folding of the complex C-terminal ancillary domain is the rate-limiting step in biosynthesis of ADAMTS10, and that it (but not catalytic domain) is sensitive to subtle changes in efficiency of signal peptide cleavage.
Title: Functional analysis of an ADAMTS10 signal peptide mutation in Weill-Marchesani syndrome demonstrates a long-range effect on secretion of the full-length enzyme.

The following ADAMTS10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20533 NM_001282352.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu51850 XM_006722917.3
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu88745 XM_017027339.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu88746 XM_017027340.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu20510 NM_030957.3
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $776.30
$1109.00
OHu20510 XM_017027338.1
Latest version!
Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $776.30
$1109.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20533
Clone ID Related Accession (Same CDS sequence) NM_001282352.1
Accession Version NM_001282352.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1773bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK131414.1, AF163762.1, BC029308.1, AC130469.2 and BF510205.1. On or before Aug 31, 2013 this sequence version replaced XM_005272501.1, XM_005277799.1. Transcript Variant: This variant (2) lacks several exons, uses an alternate 5'-terminal exon, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK131414.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGCCGA CGAGTGTCCT CCGGGCTGGA CTCACTCCTT CCTGTCTCCC CCCACCCAGT 
GGTGCTACAA ACGGGTCTGT GTCCCCTTTG GGTCGCGCCC AGAGGGTGTG GACGGAGCCT
GGGGGCCGTG GACTCCATGG GGCGACTGCA GCCGGACCTG TGGCGGCGGC GTGTCCTCTT
CTAGCCGTCA CTGCGACAGC CCCAGGCCAA CCATCGGGGG CAAGTACTGT CTGGGTGAGA
GAAGGCGGCA CCGCTCCTGC AACACGGATG ACTGTCCCCC TGGCTCCCAG GACTTCAGAG
AAGTGCAGTG TTCTGAATTT GACAGCATCC CTTTCCGTGG GAAATTCTAC AAGTGGAAAA
CGTACCGGGG AGGCCTGCTC GCTCACGTGC CTAGCGGAAG GCTTCAACTT CTACACGGAG
AGGGCGGCAG CCGTGGTGGA CGGGACACCC TGCCGTCCAG ACACGGTGGA CATTTGCGTC
AGTGGCGAAT GCAAGCACGT GGGCTGCGAC CGAGTCCTGG GCTCCGACCT GCGGGAGGAC
AAGTGCCGAG TGTGTGGCGG TGACGGCAGT GCCTGCGAGA CCATCGAGGG CGTCTTCAGC
CCAGCCTCAC CTGGGGCCGG GTACGAGGAT GTCGTCTGGA TTCCCAAAGG CTCCGTCCAC
ATCTTCATCC AGGATCTGAA CCTCTCTCTC AGTCACTTGG CCCTGAAGGG AGACCAGGAG
TCCCTGCTGC TGGAGGGGCT GCCTGGGACC CCCCAGCCCC ACCGTCTGCC TCTAGCTGGG
ACCACCTTTC AACTGCGACA GGGGCCAGAC CAGGTCCAGA GCCTCGAAGC CCTGGGACCG
ATTAATGCAT CTCTCATCGT CATGGTGCTG GCCCGGACCG AGCTGCCTGC CCTCCGCTAC
CGCTTCAATG CCCCCATCGC CCGTGACTCG CTGCCCCCCT ACTCCTGGCA CTATGCGCCC
TGGACCAAGT GCTCGGCCCA GTGTGCAGGC GGTAGCCAGG TGCAGGCGGT GGAGTGCCGC
AACCAGCTGG ACAGCTCCGC GGTCGCCCCC CACTACTGCA GTGCCCACAG CAAGCTGCCC
AAAAGGCAGC GCGCCTGCAA CACGGAGCCT TGCCCTCCAG ACTGGGTTGT AGGGAACTGG
TCGCTCTGCA GCCGCAGCTG CGATGCAGGC GTGCGCAGCC GCTCGGTCGT GTGCCAGCGC
CGCGTCTCTG CCGCGGAGGA GAAGGCGCTG GACGACAGCG CATGCCCGCA GCCGCGCCCA
CCTGTACTGG AGGCCTGCCA CGGCCCCACT TGCCCTCCGG AGTGGGCGGC CCTCGACTGG
TCTGAGTGCA CCCCCAGCTG CGGGCCGGGC CTCCGCCACC GCGTGGTCCT TTGCAAGAGC
GCAGACCACC GCGCCACGCT GCCCCCGGCG CACTGCTCAC CCGCCGCCAA GCCACCGGCC
ACCATGCGCT GCAACTTGCG CCGCTGCCCC CCGGCCCGCT GGGTGGCTGG CGAGTGGGGT
GAGTGCTCTG CACAGTGCGG CGTCGGGCAG CGGCAGCGCT CGGTGCGCTG CACCAGCCAC
ACGGGCCAGG CGTCGCACGA GTGCACGGAG GCCCTGCGGC CGCCCACCAC GCAGCAGTGT
GAGGCCAAGT GCGACAGCCC AACCCCCGGG GACGGCCCTG AAGAGTGCAA GGATGTGAAC
AAGGTCGCCT ACTGCCCCCT GGTGCTCAAA TTTCAGTTCT GCAGCCGAGC CTACTTCCGC
CAGATGTGCT GCAAAACCTG CCATGGCCAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269281.1
CDS35..1807
Misc Feature(1)611..949
Misc Feature(2)1349..1504
Misc Feature(3)1523..1657
Misc Feature(4)1700..1795
Exon (1)1..239
Exon (2)240..303
Exon (3)304..406
Exon (4)407..529
Exon (5)530..653
Exon (6)654..734
Exon (7)735..898
Exon (8)899..1025
Exon (9)1026..1155
Exon (10)1156..1360
Exon (11)1361..1537
Exon (12)1538..1697
Exon (13)1698..2460
Translation

Target ORF information:

RefSeq Version NM_001282352.1
Organism Homo sapiens(Human)
Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282352.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGCCGA CGAGTGTCCT CCGGGCTGGA CTCACTCCTT CCTGTCTCCC CCCACCCAGT 
GGTGCTACAA ACGGGTCTGT GTCCCCTTTG GGTCGCGCCC AGAGGGTGTG GACGGAGCCT
GGGGGCCGTG GACTCCATGG GGCGACTGCA GCCGGACCTG TGGCGGCGGC GTGTCCTCTT
CTAGCCGTCA CTGCGACAGC CCCAGGCCAA CCATCGGGGG CAAGTACTGT CTGGGTGAGA
GAAGGCGGCA CCGCTCCTGC AACACGGATG ACTGTCCCCC TGGCTCCCAG GACTTCAGAG
AAGTGCAGTG TTCTGAATTT GACAGCATCC CTTTCCGTGG GAAATTCTAC AAGTGGAAAA
CGTACCGGGG AGGCCTGCTC GCTCACGTGC CTAGCGGAAG GCTTCAACTT CTACACGGAG
AGGGCGGCAG CCGTGGTGGA CGGGACACCC TGCCGTCCAG ACACGGTGGA CATTTGCGTC
AGTGGCGAAT GCAAGCACGT GGGCTGCGAC CGAGTCCTGG GCTCCGACCT GCGGGAGGAC
AAGTGCCGAG TGTGTGGCGG TGACGGCAGT GCCTGCGAGA CCATCGAGGG CGTCTTCAGC
CCAGCCTCAC CTGGGGCCGG GTACGAGGAT GTCGTCTGGA TTCCCAAAGG CTCCGTCCAC
ATCTTCATCC AGGATCTGAA CCTCTCTCTC AGTCACTTGG CCCTGAAGGG AGACCAGGAG
TCCCTGCTGC TGGAGGGGCT GCCTGGGACC CCCCAGCCCC ACCGTCTGCC TCTAGCTGGG
ACCACCTTTC AACTGCGACA GGGGCCAGAC CAGGTCCAGA GCCTCGAAGC CCTGGGACCG
ATTAATGCAT CTCTCATCGT CATGGTGCTG GCCCGGACCG AGCTGCCTGC CCTCCGCTAC
CGCTTCAATG CCCCCATCGC CCGTGACTCG CTGCCCCCCT ACTCCTGGCA CTATGCGCCC
TGGACCAAGT GCTCGGCCCA GTGTGCAGGC GGTAGCCAGG TGCAGGCGGT GGAGTGCCGC
AACCAGCTGG ACAGCTCCGC GGTCGCCCCC CACTACTGCA GTGCCCACAG CAAGCTGCCC
AAAAGGCAGC GCGCCTGCAA CACGGAGCCT TGCCCTCCAG ACTGGGTTGT AGGGAACTGG
TCGCTCTGCA GCCGCAGCTG CGATGCAGGC GTGCGCAGCC GCTCGGTCGT GTGCCAGCGC
CGCGTCTCTG CCGCGGAGGA GAAGGCGCTG GACGACAGCG CATGCCCGCA GCCGCGCCCA
CCTGTACTGG AGGCCTGCCA CGGCCCCACT TGCCCTCCGG AGTGGGCGGC CCTCGACTGG
TCTGAGTGCA CCCCCAGCTG CGGGCCGGGC CTCCGCCACC GCGTGGTCCT TTGCAAGAGC
GCAGACCACC GCGCCACGCT GCCCCCGGCG CACTGCTCAC CCGCCGCCAA GCCACCGGCC
ACCATGCGCT GCAACTTGCG CCGCTGCCCC CCGGCCCGCT GGGTGGCTGG CGAGTGGGGT
GAGTGCTCTG CACAGTGCGG CGTCGGGCAG CGGCAGCGCT CGGTGCGCTG CACCAGCCAC
ACGGGCCAGG CGTCGCACGA GTGCACGGAG GCCCTGCGGC CGCCCACCAC GCAGCAGTGT
GAGGCCAAGT GCGACAGCCC AACCCCCGGG GACGGCCCTG AAGAGTGCAA GGATGTGAAC
AAGGTCGCCT ACTGCCCCCT GGTGCTCAAA TTTCAGTTCT GCAGCCGAGC CTACTTCCGC
CAGATGTGCT GCAAAACCTG CCATGGCCAC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51850
Clone ID Related Accession (Same CDS sequence) XM_006722917.3
Accession Version XM_006722917.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006722917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGTGTGAGC GCGAGAGAAG CTGCAGCGTC AATGAGGACA TTGGCCTGGC CACAGCGTTC 
ACCATTGCCC ACGAGATCGG GCACACATTC GGCATGAACC ATGACGGCGT GGGAAACAGC
TGTGGGGCCC GTGGTCAGGA CCCAGCCAAG CTCATGGCTG CCCACATTAC CATGAAGACC
AACCCATTCG TGTGGTCATC CTGCAGCCGT GACTACATCA CCAGCTTTCT AGACTCGGGC
CTGGGGCTCT GCCTGAACAA CCGGCCCCCC AGACAGGACT TTGTGTACCC GACAGTGGCA
CCGGGCCAAG CCTACGATGC AGATGAGCAA TGCCGCTTTC AGCATGGAGT CAAATCGCGT
CAGTGTAAAT ACGGGGAGGT CTGCAGCGAG CTGTGGTGTC TGAGCAAGAG CAACCGGTGC
ATCACCAACA GCATCCCGGC CGCCGAGGGC ACGCTGTGCC AGACGCACAC CATCGACAAG
GGGTGGTGCT ACAAACGGGT CTGTGTCCCC TTTGGGTCGC GCCCAGAGGG TGTGGACGGA
GCCTGGGGGC CGTGGACTCC ATGGGGCGAC TGCAGCCGGA CCTGTGGCGG CGGCGTGTCC
TCTTCTAGCC GTCACTGCGA CAGCCCCAGG CCAACCATCG GGGGCAAGTA CTGTCTGGGT
GAGAGAAGGC GGCACCGCTC CTGCAACACG GATGACTGTC CCCCTGGCTC CCAGGACTTC
AGAGAAGTGC AGTGTTCTGA ATTTGACAGC ATCCCTTTCC GTGGGAAATT CTACAAGTGG
AAAACGTACC GGGGAGGGGG CGTGAAGGCC TGCTCGCTCA CGTGCCTAGC GGAAGGCTTC
AACTTCTACA CGGAGAGGGC GGCAGCCGTG GTGGACGGGA CACCCTGCCG TCCAGACACG
GTGGACATTT GCGTCAGTGG CGAATGCAAG CACGTGGGCT GCGACCGAGT CCTGGGCTCC
GACCTGCGGG AGGACAAGTG CCGAGTGTGT GGCGGTGACG GCAGTGCCTG CGAGACCATC
GAGGGCGTCT TCAGCCCAGC CTCACCTGGG GCCGGGTACG AGGATGTCGT CTGGATTCCC
AAAGGCTCCG TCCACATCTT CATCCAGGAT CTGAACCTCT CTCTCAGTCA CTTGGCCCTG
AAGGGAGACC AGGAGTCCCT GCTGCTGGAG GGGCTGCCTG GGACCCCCCA GCCCCACCGT
CTGCCTCTAG CTGGGACCAC CTTTCAACTG CGACAGGGGC CAGACCAGGT CCAGAGCCTC
GAAGCCCTGG GACCGATTAA TGCATCTCTC ATCGTCATGG TGCTGGCCCG GACCGAGCTG
CCTGCCCTCC GCTACCGCTT CAATGCCCCC ATCGCCCGTG ACTCGCTGCC CCCCTACTCC
TGGCACTATG CGCCCTGGAC CAAGTGCTCG GCCCAGTGTG CAGGCGGTAG CCAGGTGCAG
GCGGTGGAGT GCCGCAACCA GCTGGACAGC TCCGCGGTCG CCCCCCACTA CTGCAGTGCC
CACAGCAAGC TGCCCAAAAG GCAGCGCGCC TGCAACACGG AGCCTTGCCC TCCAGACTGG
GTTGTAGGGA ACTGGTCGCT CTGCAGCCGC AGCTGCGATG CAGGCGTGCG CAGCCGCTCG
GTCGTGTGCC AGCGCCGCGT CTCTGCCGCG GAGGAGAAGG CGCTGGACGA CAGCGCATGC
CCGCAGCCGC GCCCACCTGT ACTGGAGGCC TGCCACGGCC CCACTTGCCC TCCGGAGTGG
GCGGCCCTCG ACTGGTCTGA GGTCAGCCGC CCCCTTCCTT CGCGCCCACT GGGATGCCCA
GGTGGGGTGT CCACGGAGAC GCCTCTGCGC GGTCTCCAGG TTAGCTGTCC CCTCCTCACT
TCGCACTGGG GCAGCACCCT GAGCACGAAA CGCCCCTTCC AACTGCAGTG CACCCCCAGC
TGCGGGCCGG GCCTCCGCCA CCGCGTGGTC CTTTGCAAGA GCGCAGACCA CCGCGCCACG
CTGCCCCCGG CGCACTGCTC ACCCGCCGCC AAGCCACCGG CCACCATGCG CTGCAACTTG
CGCCGCTGCC CCCCGGCCCG CTGGGTGGCT GGCGAGTGGG GTGAGTGCTC TGCACAGTGC
GGCGTCGGGC AGCGGCAGCG CTCGGTGCGC TGCACCAGCC ACACGGGCCA GGCGTCGCAC
GAGTGCACGG AGGCCCTGCG GCCGCCCACC ACGCAGCAGT GTGAGGCCAA GTGCGACAGC
CCAACCCCCG GGGACGGCCC TGAAGAGTGC AAGGATGTGA ACAAGGTCGC CTACTGCCCC
CTGGTGCTCA AATTTCAGTT CTGCAGCCGA GCCTACTTCC GCCAGATGTG CTGCAAAACC
TGCCATGGCC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722980.1
CDS1257..3611
Misc Feature(1)<1257..1514
Misc Feature(2)order(1326..1331,1338..1340,1356..1358)
Misc Feature(3)1800..1958
Misc Feature(4)2268..2606
Misc Feature(5)3327..3461
Misc Feature(6)3504..3599
Translation

Target ORF information:

RefSeq Version XM_006722917.3
Organism Homo sapiens(Human)
Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006722917.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGTGTGAGC GCGAGAGAAG CTGCAGCGTC AATGAGGACA TTGGCCTGGC CACAGCGTTC 
ACCATTGCCC ACGAGATCGG GCACACATTC GGCATGAACC ATGACGGCGT GGGAAACAGC
TGTGGGGCCC GTGGTCAGGA CCCAGCCAAG CTCATGGCTG CCCACATTAC CATGAAGACC
AACCCATTCG TGTGGTCATC CTGCAGCCGT GACTACATCA CCAGCTTTCT AGACTCGGGC
CTGGGGCTCT GCCTGAACAA CCGGCCCCCC AGACAGGACT TTGTGTACCC GACAGTGGCA
CCGGGCCAAG CCTACGATGC AGATGAGCAA TGCCGCTTTC AGCATGGAGT CAAATCGCGT
CAGTGTAAAT ACGGGGAGGT CTGCAGCGAG CTGTGGTGTC TGAGCAAGAG CAACCGGTGC
ATCACCAACA GCATCCCGGC CGCCGAGGGC ACGCTGTGCC AGACGCACAC CATCGACAAG
GGGTGGTGCT ACAAACGGGT CTGTGTCCCC TTTGGGTCGC GCCCAGAGGG TGTGGACGGA
GCCTGGGGGC CGTGGACTCC ATGGGGCGAC TGCAGCCGGA CCTGTGGCGG CGGCGTGTCC
TCTTCTAGCC GTCACTGCGA CAGCCCCAGG CCAACCATCG GGGGCAAGTA CTGTCTGGGT
GAGAGAAGGC GGCACCGCTC CTGCAACACG GATGACTGTC CCCCTGGCTC CCAGGACTTC
AGAGAAGTGC AGTGTTCTGA ATTTGACAGC ATCCCTTTCC GTGGGAAATT CTACAAGTGG
AAAACGTACC GGGGAGGGGG CGTGAAGGCC TGCTCGCTCA CGTGCCTAGC GGAAGGCTTC
AACTTCTACA CGGAGAGGGC GGCAGCCGTG GTGGACGGGA CACCCTGCCG TCCAGACACG
GTGGACATTT GCGTCAGTGG CGAATGCAAG CACGTGGGCT GCGACCGAGT CCTGGGCTCC
GACCTGCGGG AGGACAAGTG CCGAGTGTGT GGCGGTGACG GCAGTGCCTG CGAGACCATC
GAGGGCGTCT TCAGCCCAGC CTCACCTGGG GCCGGGTACG AGGATGTCGT CTGGATTCCC
AAAGGCTCCG TCCACATCTT CATCCAGGAT CTGAACCTCT CTCTCAGTCA CTTGGCCCTG
AAGGGAGACC AGGAGTCCCT GCTGCTGGAG GGGCTGCCTG GGACCCCCCA GCCCCACCGT
CTGCCTCTAG CTGGGACCAC CTTTCAACTG CGACAGGGGC CAGACCAGGT CCAGAGCCTC
GAAGCCCTGG GACCGATTAA TGCATCTCTC ATCGTCATGG TGCTGGCCCG GACCGAGCTG
CCTGCCCTCC GCTACCGCTT CAATGCCCCC ATCGCCCGTG ACTCGCTGCC CCCCTACTCC
TGGCACTATG CGCCCTGGAC CAAGTGCTCG GCCCAGTGTG CAGGCGGTAG CCAGGTGCAG
GCGGTGGAGT GCCGCAACCA GCTGGACAGC TCCGCGGTCG CCCCCCACTA CTGCAGTGCC
CACAGCAAGC TGCCCAAAAG GCAGCGCGCC TGCAACACGG AGCCTTGCCC TCCAGACTGG
GTTGTAGGGA ACTGGTCGCT CTGCAGCCGC AGCTGCGATG CAGGCGTGCG CAGCCGCTCG
GTCGTGTGCC AGCGCCGCGT CTCTGCCGCG GAGGAGAAGG CGCTGGACGA CAGCGCATGC
CCGCAGCCGC GCCCACCTGT ACTGGAGGCC TGCCACGGCC CCACTTGCCC TCCGGAGTGG
GCGGCCCTCG ACTGGTCTGA GGTCAGCCGC CCCCTTCCTT CGCGCCCACT GGGATGCCCA
GGTGGGGTGT CCACGGAGAC GCCTCTGCGC GGTCTCCAGG TTAGCTGTCC CCTCCTCACT
TCGCACTGGG GCAGCACCCT GAGCACGAAA CGCCCCTTCC AACTGCAGTG CACCCCCAGC
TGCGGGCCGG GCCTCCGCCA CCGCGTGGTC CTTTGCAAGA GCGCAGACCA CCGCGCCACG
CTGCCCCCGG CGCACTGCTC ACCCGCCGCC AAGCCACCGG CCACCATGCG CTGCAACTTG
CGCCGCTGCC CCCCGGCCCG CTGGGTGGCT GGCGAGTGGG GTGAGTGCTC TGCACAGTGC
GGCGTCGGGC AGCGGCAGCG CTCGGTGCGC TGCACCAGCC ACACGGGCCA GGCGTCGCAC
GAGTGCACGG AGGCCCTGCG GCCGCCCACC ACGCAGCAGT GTGAGGCCAA GTGCGACAGC
CCAACCCCCG GGGACGGCCC TGAAGAGTGC AAGGATGTGA ACAAGGTCGC CTACTGCCCC
CTGGTGCTCA AATTTCAGTT CTGCAGCCGA GCCTACTTCC GCCAGATGTG CTGCAAAACC
TGCCATGGCC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu88745
Clone ID Related Accession (Same CDS sequence) XM_017027339.1
Accession Version XM_017027339.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGATGA GCAATGCCGC TTTCAGCATG GAGTCAAATC GCGTCAGTGA GGTCTGCAGC 
GAGCTGTGGT GTCTGAGCAA GAGCAACCGG TGCATCACCA ACAGCATCCC GGCCGCCGAG
GGCACGCTGT GCCAGACGCA CACCATCGAC AAGGGGTGGT GCTACAAACG GGTCTGTGTC
CCCTTTGGGT CGCGCCCAGA GGGTGTGGAC GGAGCCTGGG GGCCGTGGAC TCCATGGGGC
GACTGCAGCC GGACCTGTGG CGGCGGCGTG TCCTCTTCTA GCCGTCACTG CGACAGCCCC
AGGCCAACCA TCGGGGGCAA GTACTGTCTG GGTGAGAGAA GGCGGCACCG CTCCTGCAAC
ACGGATGACT GTCCCCCTGG CTCCCAGGAC TTCAGAGAAG TGCAGTGTTC TGAATTTGAC
AGCATCCCTT TCCGTGGGAA ATTCTACAAG TGGAAAACGT ACCGGGGAGG GGGCGTGAAG
GCCTGCTCGC TCACGTGCCT AGCGGAAGGC TTCAACTTCT ACACGGAGAG GGCGGCAGCC
GTGGTGGACG GGACACCCTG CCGTCCAGAC ACGGTGGACA TTTGCGTCAG TGGCGAATGC
AAGCACGTGG GCTGCGACCG AGTCCTGGGC TCCGACCTGC GGGAGGACAA GTGCCGAGTG
TGTGGCGGTG ACGGCAGTGC CTGCGAGACC ATCGAGGGCG TCTTCAGCCC AGCCTCACCT
GGGGCCGGGT ACGAGGATGT CGTCTGGATT CCCAAAGGCT CCGTCCACAT CTTCATCCAG
GATCTGAACC TCTCTCTCAG TCACTTGGCC CTGAAGGGAG ACCAGGAGTC CCTGCTGCTG
GAGGGGCTGC CTGGGACCCC CCAGCCCCAC CGTCTGCCTC TAGCTGGGAC CACCTTTCAA
CTGCGACAGG GGCCAGACCA GGTCCAGAGC CTCGAAGCCC TGGGACCGAT TAATGCATCT
CTCATCGTCA TGGTGCTGGC CCGGACCGAG CTGCCTGCCC TCCGCTACCG CTTCAATGCC
CCCATCGCCC GTGACTCGCT GCCCCCCTAC TCCTGGCACT ATGCGCCCTG GACCAAGTGC
TCGGCCCAGT GTGCAGGCGG TAGCCAGGTG CAGGCGGTGG AGTGCCGCAA CCAGCTGGAC
AGCTCCGCGG TCGCCCCCCA CTACTGCAGT GCCCACAGCA AGCTGCCCAA AAGGCAGCGC
GCCTGCAACA CGGAGCCTTG CCCTCCAGAC TGGGTTGTAG GGAACTGGTC GCTCTGCAGC
CGCAGCTGCG ATGCAGGCGT GCGCAGCCGC TCGGTCGTGT GCCAGCGCCG CGTCTCTGCC
GCGGAGGAGA AGGCGCTGGA CGACAGCGCA TGCCCGCAGC CGCGCCCACC TGTACTGGAG
GCCTGCCACG GCCCCACTTG CCCTCCGGAG TGGGCGGCCC TCGACTGGTC TGAGTGCACC
CCCAGCTGCG GGCCGGGCCT CCGCCACCGC GTGGTCCTTT GCAAGAGCGC AGACCACCGC
GCCACGCTGC CCCCGGCGCA CTGCTCACCC GCCGCCAAGC CACCGGCCAC CATGCGCTGC
AACTTGCGCC GCTGCCCCCC GGCCCGCTGG GTGGCTGGCG AGTGGGGTGA GTGCTCTGCA
CAGTGCGGCG TCGGGCAGCG GCAGCGCTCG GTGCGCTGCA CCAGCCACAC GGGCCAGGCG
TCGCACGAGT GCACGGAGGC CCTGCGGCCG CCCACCACGC AGCAGTGTGA GGCCAAGTGC
GACAGCCCAA CCCCCGGGGA CGGCCCTGAA GAGTGCAAGG ATGTGAACAA GGTCGCCTAC
TGCCCCCTGG TGCTCAAATT TCAGTTCTGC AGCCGAGCCT ACTTCCGCCA GATGTGCTGC
AAAACCTGCC ATGGCCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016882828.1
CDS218..2098
Translation

Target ORF information:

RefSeq Version XM_017027339.1
Organism Homo sapiens(Human)
Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027339.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGATGA GCAATGCCGC TTTCAGCATG GAGTCAAATC GCGTCAGTGA GGTCTGCAGC 
GAGCTGTGGT GTCTGAGCAA GAGCAACCGG TGCATCACCA ACAGCATCCC GGCCGCCGAG
GGCACGCTGT GCCAGACGCA CACCATCGAC AAGGGGTGGT GCTACAAACG GGTCTGTGTC
CCCTTTGGGT CGCGCCCAGA GGGTGTGGAC GGAGCCTGGG GGCCGTGGAC TCCATGGGGC
GACTGCAGCC GGACCTGTGG CGGCGGCGTG TCCTCTTCTA GCCGTCACTG CGACAGCCCC
AGGCCAACCA TCGGGGGCAA GTACTGTCTG GGTGAGAGAA GGCGGCACCG CTCCTGCAAC
ACGGATGACT GTCCCCCTGG CTCCCAGGAC TTCAGAGAAG TGCAGTGTTC TGAATTTGAC
AGCATCCCTT TCCGTGGGAA ATTCTACAAG TGGAAAACGT ACCGGGGAGG GGGCGTGAAG
GCCTGCTCGC TCACGTGCCT AGCGGAAGGC TTCAACTTCT ACACGGAGAG GGCGGCAGCC
GTGGTGGACG GGACACCCTG CCGTCCAGAC ACGGTGGACA TTTGCGTCAG TGGCGAATGC
AAGCACGTGG GCTGCGACCG AGTCCTGGGC TCCGACCTGC GGGAGGACAA GTGCCGAGTG
TGTGGCGGTG ACGGCAGTGC CTGCGAGACC ATCGAGGGCG TCTTCAGCCC AGCCTCACCT
GGGGCCGGGT ACGAGGATGT CGTCTGGATT CCCAAAGGCT CCGTCCACAT CTTCATCCAG
GATCTGAACC TCTCTCTCAG TCACTTGGCC CTGAAGGGAG ACCAGGAGTC CCTGCTGCTG
GAGGGGCTGC CTGGGACCCC CCAGCCCCAC CGTCTGCCTC TAGCTGGGAC CACCTTTCAA
CTGCGACAGG GGCCAGACCA GGTCCAGAGC CTCGAAGCCC TGGGACCGAT TAATGCATCT
CTCATCGTCA TGGTGCTGGC CCGGACCGAG CTGCCTGCCC TCCGCTACCG CTTCAATGCC
CCCATCGCCC GTGACTCGCT GCCCCCCTAC TCCTGGCACT ATGCGCCCTG GACCAAGTGC
TCGGCCCAGT GTGCAGGCGG TAGCCAGGTG CAGGCGGTGG AGTGCCGCAA CCAGCTGGAC
AGCTCCGCGG TCGCCCCCCA CTACTGCAGT GCCCACAGCA AGCTGCCCAA AAGGCAGCGC
GCCTGCAACA CGGAGCCTTG CCCTCCAGAC TGGGTTGTAG GGAACTGGTC GCTCTGCAGC
CGCAGCTGCG ATGCAGGCGT GCGCAGCCGC TCGGTCGTGT GCCAGCGCCG CGTCTCTGCC
GCGGAGGAGA AGGCGCTGGA CGACAGCGCA TGCCCGCAGC CGCGCCCACC TGTACTGGAG
GCCTGCCACG GCCCCACTTG CCCTCCGGAG TGGGCGGCCC TCGACTGGTC TGAGTGCACC
CCCAGCTGCG GGCCGGGCCT CCGCCACCGC GTGGTCCTTT GCAAGAGCGC AGACCACCGC
GCCACGCTGC CCCCGGCGCA CTGCTCACCC GCCGCCAAGC CACCGGCCAC CATGCGCTGC
AACTTGCGCC GCTGCCCCCC GGCCCGCTGG GTGGCTGGCG AGTGGGGTGA GTGCTCTGCA
CAGTGCGGCG TCGGGCAGCG GCAGCGCTCG GTGCGCTGCA CCAGCCACAC GGGCCAGGCG
TCGCACGAGT GCACGGAGGC CCTGCGGCCG CCCACCACGC AGCAGTGTGA GGCCAAGTGC
GACAGCCCAA CCCCCGGGGA CGGCCCTGAA GAGTGCAAGG ATGTGAACAA GGTCGCCTAC
TGCCCCCTGG TGCTCAAATT TCAGTTCTGC AGCCGAGCCT ACTTCCGCCA GATGTGCTGC
AAAACCTGCC ATGGCCACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu88746
Clone ID Related Accession (Same CDS sequence) XM_017027340.1
Accession Version XM_017027340.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGATGA GCAATGCCGC TTTCAGCATG GAGTCAAATC GCGAGGTCTG CAGCGAGCTG 
TGGTGTCTGA GCAAGAGCAA CCGGTGCATC ACCAACAGCA TCCCGGCCGC CGAGGGCACG
CTGTGCCAGA CGCACACCAT CGACAAGGGG TGGTGCTACA AACGGGTCTG TGTCCCCTTT
GGGTCGCGCC CAGAGGGTGT GGACGGAGCC TGGGGGCCGT GGACTCCATG GGGCGACTGC
AGCCGGACCT GTGGCGGCGG CGTGTCCTCT TCTAGCCGTC ACTGCGACAG CCCCAGGCCA
ACCATCGGGG GCAAGTACTG TCTGGGTGAG AGAAGGCGGC ACCGCTCCTG CAACACGGAT
GACTGTCCCC CTGGCTCCCA GGACTTCAGA GAAGTGCAGT GTTCTGAATT TGACAGCATC
CCTTTCCGTG GGAAATTCTA CAAGTGGAAA ACGTACCGGG GAGGGGGCGT GAAGGCCTGC
TCGCTCACGT GCCTAGCGGA AGGCTTCAAC TTCTACACGG AGAGGGCGGC AGCCGTGGTG
GACGGGACAC CCTGCCGTCC AGACACGGTG GACATTTGCG TCAGTGGCGA ATGCAAGCAC
GTGGGCTGCG ACCGAGTCCT GGGCTCCGAC CTGCGGGAGG ACAAGTGCCG AGTGTGTGGC
GGTGACGGCA GTGCCTGCGA GACCATCGAG GGCGTCTTCA GCCCAGCCTC ACCTGGGGCC
GGGTACGAGG ATGTCGTCTG GATTCCCAAA GGCTCCGTCC ACATCTTCAT CCAGGATCTG
AACCTCTCTC TCAGTCACTT GGCCCTGAAG GGAGACCAGG AGTCCCTGCT GCTGGAGGGG
CTGCCTGGGA CCCCCCAGCC CCACCGTCTG CCTCTAGCTG GGACCACCTT TCAACTGCGA
CAGGGGCCAG ACCAGGTCCA GAGCCTCGAA GCCCTGGGAC CGATTAATGC ATCTCTCATC
GTCATGGTGC TGGCCCGGAC CGAGCTGCCT GCCCTCCGCT ACCGCTTCAA TGCCCCCATC
GCCCGTGACT CGCTGCCCCC CTACTCCTGG CACTATGCGC CCTGGACCAA GTGCTCGGCC
CAGTGTGCAG GCGGTAGCCA GGTGCAGGCG GTGGAGTGCC GCAACCAGCT GGACAGCTCC
GCGGTCGCCC CCCACTACTG CAGTGCCCAC AGCAAGCTGC CCAAAAGGCA GCGCGCCTGC
AACACGGAGC CTTGCCCTCC AGACTGGGTT GTAGGGAACT GGTCGCTCTG CAGCCGCAGC
TGCGATGCAG GCGTGCGCAG CCGCTCGGTC GTGTGCCAGC GCCGCGTCTC TGCCGCGGAG
GAGAAGGCGC TGGACGACAG CGCATGCCCG CAGCCGCGCC CACCTGTACT GGAGGCCTGC
CACGGCCCCA CTTGCCCTCC GGAGTGGGCG GCCCTCGACT GGTCTGAGTG CACCCCCAGC
TGCGGGCCGG GCCTCCGCCA CCGCGTGGTC CTTTGCAAGA GCGCAGACCA CCGCGCCACG
CTGCCCCCGG CGCACTGCTC ACCCGCCGCC AAGCCACCGG CCACCATGCG CTGCAACTTG
CGCCGCTGCC CCCCGGCCCG CTGGGTGGCT GGCGAGTGGG GTGAGTGCTC TGCACAGTGC
GGCGTCGGGC AGCGGCAGCG CTCGGTGCGC TGCACCAGCC ACACGGGCCA GGCGTCGCAC
GAGTGCACGG AGGCCCTGCG GCCGCCCACC ACGCAGCAGT GTGAGGCCAA GTGCGACAGC
CCAACCCCCG GGGACGGCCC TGAAGAGTGC AAGGATGTGA ACAAGGTCGC CTACTGCCCC
CTGGTGCTCA AATTTCAGTT CTGCAGCCGA GCCTACTTCC GCCAGATGTG CTGCAAAACC
TGCCATGGCC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016882829.1
CDS84..1958
Translation

Target ORF information:

RefSeq Version XM_017027340.1
Organism Homo sapiens(Human)
Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027340.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGCAGATGA GCAATGCCGC TTTCAGCATG GAGTCAAATC GCGAGGTCTG CAGCGAGCTG 
TGGTGTCTGA GCAAGAGCAA CCGGTGCATC ACCAACAGCA TCCCGGCCGC CGAGGGCACG
CTGTGCCAGA CGCACACCAT CGACAAGGGG TGGTGCTACA AACGGGTCTG TGTCCCCTTT
GGGTCGCGCC CAGAGGGTGT GGACGGAGCC TGGGGGCCGT GGACTCCATG GGGCGACTGC
AGCCGGACCT GTGGCGGCGG CGTGTCCTCT TCTAGCCGTC ACTGCGACAG CCCCAGGCCA
ACCATCGGGG GCAAGTACTG TCTGGGTGAG AGAAGGCGGC ACCGCTCCTG CAACACGGAT
GACTGTCCCC CTGGCTCCCA GGACTTCAGA GAAGTGCAGT GTTCTGAATT TGACAGCATC
CCTTTCCGTG GGAAATTCTA CAAGTGGAAA ACGTACCGGG GAGGGGGCGT GAAGGCCTGC
TCGCTCACGT GCCTAGCGGA AGGCTTCAAC TTCTACACGG AGAGGGCGGC AGCCGTGGTG
GACGGGACAC CCTGCCGTCC AGACACGGTG GACATTTGCG TCAGTGGCGA ATGCAAGCAC
GTGGGCTGCG ACCGAGTCCT GGGCTCCGAC CTGCGGGAGG ACAAGTGCCG AGTGTGTGGC
GGTGACGGCA GTGCCTGCGA GACCATCGAG GGCGTCTTCA GCCCAGCCTC ACCTGGGGCC
GGGTACGAGG ATGTCGTCTG GATTCCCAAA GGCTCCGTCC ACATCTTCAT CCAGGATCTG
AACCTCTCTC TCAGTCACTT GGCCCTGAAG GGAGACCAGG AGTCCCTGCT GCTGGAGGGG
CTGCCTGGGA CCCCCCAGCC CCACCGTCTG CCTCTAGCTG GGACCACCTT TCAACTGCGA
CAGGGGCCAG ACCAGGTCCA GAGCCTCGAA GCCCTGGGAC CGATTAATGC ATCTCTCATC
GTCATGGTGC TGGCCCGGAC CGAGCTGCCT GCCCTCCGCT ACCGCTTCAA TGCCCCCATC
GCCCGTGACT CGCTGCCCCC CTACTCCTGG CACTATGCGC CCTGGACCAA GTGCTCGGCC
CAGTGTGCAG GCGGTAGCCA GGTGCAGGCG GTGGAGTGCC GCAACCAGCT GGACAGCTCC
GCGGTCGCCC CCCACTACTG CAGTGCCCAC AGCAAGCTGC CCAAAAGGCA GCGCGCCTGC
AACACGGAGC CTTGCCCTCC AGACTGGGTT GTAGGGAACT GGTCGCTCTG CAGCCGCAGC
TGCGATGCAG GCGTGCGCAG CCGCTCGGTC GTGTGCCAGC GCCGCGTCTC TGCCGCGGAG
GAGAAGGCGC TGGACGACAG CGCATGCCCG CAGCCGCGCC CACCTGTACT GGAGGCCTGC
CACGGCCCCA CTTGCCCTCC GGAGTGGGCG GCCCTCGACT GGTCTGAGTG CACCCCCAGC
TGCGGGCCGG GCCTCCGCCA CCGCGTGGTC CTTTGCAAGA GCGCAGACCA CCGCGCCACG
CTGCCCCCGG CGCACTGCTC ACCCGCCGCC AAGCCACCGG CCACCATGCG CTGCAACTTG
CGCCGCTGCC CCCCGGCCCG CTGGGTGGCT GGCGAGTGGG GTGAGTGCTC TGCACAGTGC
GGCGTCGGGC AGCGGCAGCG CTCGGTGCGC TGCACCAGCC ACACGGGCCA GGCGTCGCAC
GAGTGCACGG AGGCCCTGCG GCCGCCCACC ACGCAGCAGT GTGAGGCCAA GTGCGACAGC
CCAACCCCCG GGGACGGCCC TGAAGAGTGC AAGGATGTGA ACAAGGTCGC CTACTGCCCC
CTGGTGCTCA AATTTCAGTT CTGCAGCCGA GCCTACTTCC GCCAGATGTG CTGCAAAACC
TGCCATGGCC ACTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20510
Clone ID Related Accession (Same CDS sequence) XM_017027338.1 , NM_030957.3
Accession Version NM_030957.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 1 preproprotein
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN431169.1, AB209515.1, AF163762.1, BC029308.1, AC130469.2, AK131414.1 and BF510205.1. This sequence is a reference standard in the RefSeqGene project. On Aug 31, 2013 this sequence version replaced NM_030957.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCTCCCG CCTGCCAGAT CCTCCGCTGG GCCCTCGCCC TGGGGCTGGG CCTCATGTTC 
GAGGTCACGC ACGCCTTCCG GTCTCAAGAT GAGTTCCTGT CCAGTCTGGA GAGCTATGAG
ATCGCCTTCC CCACCCGCGT GGACCACAAC GGGGCACTGC TGGCCTTCTC GCCACCTCCT
CCCCGGAGGC AGCGCCGCGG CACGGGGGCC ACAGCCGAGT CCCGCCTCTT CTACAAAGTG
GCCTCGCCCA GCACCCACTT CCTGCTGAAC CTGACCCGCA GCTCCCGTCT ACTGGCAGGG
CACGTCTCCG TGGAGTACTG GACACGGGAG GGCCTGGCCT GGCAGAGGGC GGCCCGGCCC
CACTGCCTCT ACGCTGGTCA CCTGCAGGGC CAGGCCAGCA GCTCCCATGT GGCCATCAGC
ACCTGTGGAG GCCTGCACGG CCTGATCGTG GCAGACGAGG AAGAGTACCT GATTGAGCCC
CTGCACGGTG GGCCCAAGGG TTCTCGGAGC CCGGAGGAAA GTGGACCACA TGTGGTGTAC
AAGCGTTCCT CTCTGCGTCA CCCCCACCTG GACACAGCCT GTGGAGTGAG AGATGAGAAA
CCGTGGAAAG GGCGGCCATG GTGGCTGCGG ACCTTGAAGC CACCGCCTGC CAGGCCCCTG
GGGAATGAAA CAGAGCGTGG CCAGCCAGGC CTGAAGCGAT CGGTCAGCCG AGAGCGCTAC
GTGGAGACCC TGGTGGTGGC TGACAAGATG ATGGTGGCCT ATCACGGGCG CCGGGATGTG
GAGCAGTATG TCCTGGCCAT CATGAACATT GTTGCCAAAC TTTTCCAGGA CTCGAGTCTG
GGAAGCACCG TTAACATCCT CGTAACTCGC CTCATCCTGC TCACGGAGGA CCAGCCCACT
CTGGAGATCA CCCACCATGC CGGGAAGTCC CTGGACAGCT TCTGTAAGTG GCAGAAATCC
ATCGTGAACC ACAGCGGCCA TGGCAATGCC ATTCCAGAGA ACGGTGTGGC TAACCATGAC
ACAGCAGTGC TCATCACACG CTATGACATC TGCATCTACA AGAACAAACC CTGCGGCACA
CTAGGCCTGG CCCCGGTGGG CGGAATGTGT GAGCGCGAGA GAAGCTGCAG CGTCAATGAG
GACATTGGCC TGGCCACAGC GTTCACCATT GCCCACGAGA TCGGGCACAC ATTCGGCATG
AACCATGACG GCGTGGGAAA CAGCTGTGGG GCCCGTGGTC AGGACCCAGC CAAGCTCATG
GCTGCCCACA TTACCATGAA GACCAACCCA TTCGTGTGGT CATCCTGCAG CCGTGACTAC
ATCACCAGCT TTCTAGACTC GGGCCTGGGG CTCTGCCTGA ACAACCGGCC CCCCAGACAG
GACTTTGTGT ACCCGACAGT GGCACCGGGC CAAGCCTACG ATGCAGATGA GCAATGCCGC
TTTCAGCATG GAGTCAAATC GCGTCAGTGT AAATACGGGG AGGTCTGCAG CGAGCTGTGG
TGTCTGAGCA AGAGCAACCG GTGCATCACC AACAGCATCC CGGCCGCCGA GGGCACGCTG
TGCCAGACGC ACACCATCGA CAAGGGGTGG TGCTACAAAC GGGTCTGTGT CCCCTTTGGG
TCGCGCCCAG AGGGTGTGGA CGGAGCCTGG GGGCCGTGGA CTCCATGGGG CGACTGCAGC
CGGACCTGTG GCGGCGGCGT GTCCTCTTCT AGCCGTCACT GCGACAGCCC CAGGCCAACC
ATCGGGGGCA AGTACTGTCT GGGTGAGAGA AGGCGGCACC GCTCCTGCAA CACGGATGAC
TGTCCCCCTG GCTCCCAGGA CTTCAGAGAA GTGCAGTGTT CTGAATTTGA CAGCATCCCT
TTCCGTGGGA AATTCTACAA GTGGAAAACG TACCGGGGAG GGGGCGTGAA GGCCTGCTCG
CTCACGTGCC TAGCGGAAGG CTTCAACTTC TACACGGAGA GGGCGGCAGC CGTGGTGGAC
GGGACACCCT GCCGTCCAGA CACGGTGGAC ATTTGCGTCA GTGGCGAATG CAAGCACGTG
GGCTGCGACC GAGTCCTGGG CTCCGACCTG CGGGAGGACA AGTGCCGAGT GTGTGGCGGT
GACGGCAGTG CCTGCGAGAC CATCGAGGGC GTCTTCAGCC CAGCCTCACC TGGGGCCGGG
TACGAGGATG TCGTCTGGAT TCCCAAAGGC TCCGTCCACA TCTTCATCCA GGATCTGAAC
CTCTCTCTCA GTCACTTGGC CCTGAAGGGA GACCAGGAGT CCCTGCTGCT GGAGGGGCTG
CCTGGGACCC CCCAGCCCCA CCGTCTGCCT CTAGCTGGGA CCACCTTTCA ACTGCGACAG
GGGCCAGACC AGGTCCAGAG CCTCGAAGCC CTGGGACCGA TTAATGCATC TCTCATCGTC
ATGGTGCTGG CCCGGACCGA GCTGCCTGCC CTCCGCTACC GCTTCAATGC CCCCATCGCC
CGTGACTCGC TGCCCCCCTA CTCCTGGCAC TATGCGCCCT GGACCAAGTG CTCGGCCCAG
TGTGCAGGCG GTAGCCAGGT GCAGGCGGTG GAGTGCCGCA ACCAGCTGGA CAGCTCCGCG
GTCGCCCCCC ACTACTGCAG TGCCCACAGC AAGCTGCCCA AAAGGCAGCG CGCCTGCAAC
ACGGAGCCTT GCCCTCCAGA CTGGGTTGTA GGGAACTGGT CGCTCTGCAG CCGCAGCTGC
GATGCAGGCG TGCGCAGCCG CTCGGTCGTG TGCCAGCGCC GCGTCTCTGC CGCGGAGGAG
AAGGCGCTGG ACGACAGCGC ATGCCCGCAG CCGCGCCCAC CTGTACTGGA GGCCTGCCAC
GGCCCCACTT GCCCTCCGGA GTGGGCGGCC CTCGACTGGT CTGAGTGCAC CCCCAGCTGC
GGGCCGGGCC TCCGCCACCG CGTGGTCCTT TGCAAGAGCG CAGACCACCG CGCCACGCTG
CCCCCGGCGC ACTGCTCACC CGCCGCCAAG CCACCGGCCA CCATGCGCTG CAACTTGCGC
CGCTGCCCCC CGGCCCGCTG GGTGGCTGGC GAGTGGGGTG AGTGCTCTGC ACAGTGCGGC
GTCGGGCAGC GGCAGCGCTC GGTGCGCTGC ACCAGCCACA CGGGCCAGGC GTCGCACGAG
TGCACGGAGG CCCTGCGGCC GCCCACCACG CAGCAGTGTG AGGCCAAGTG CGACAGCCCA
ACCCCCGGGG ACGGCCCTGA AGAGTGCAAG GATGTGAACA AGGTCGCCTA CTGCCCCCTG
GTGCTCAAAT TTCAGTTCTG CAGCCGAGCC TACTTCCGCC AGATGTGCTG CAAAACCTGC
CATGGCCACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_112219.3
CDS307..3618
Misc Feature(1)523..843
Misc Feature(2)1021..1677
Misc Feature(3)1021..1668
Misc Feature(4)order(1480..1485,1492..1494,1510..1512)
Misc Feature(5)1954..2112
Misc Feature(6)2422..2790
Misc Feature(7)2422..2760
Misc Feature(8)2791..2961
Misc Feature(9)3160..3315
Misc Feature(10)3334..3468
Misc Feature(11)3511..3606
Exon (1)1..92
Exon (2)93..207
Exon (3)208..394
Exon (4)395..741
Exon (5)742..898
Exon (6)899..1116
Exon (7)1117..1200
Exon (8)1201..1346
Exon (9)1347..1390
Exon (10)1391..1496
Exon (11)1497..1643
Exon (12)1644..1785
Exon (13)1786..1893
Exon (14)1894..2039
Exon (15)2040..2103
Exon (16)2104..2206
Exon (17)2207..2340
Exon (18)2341..2464
Exon (19)2465..2545
Exon (20)2546..2709
Exon (21)2710..2836
Exon (22)2837..2966
Exon (23)2967..3171
Exon (24)3172..3348
Exon (25)3349..3508
Exon (26)3509..4271
Translation

Target ORF information:

RefSeq Version NM_030957.3
Organism Homo sapiens(Human)
Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_030957.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCTCCCG CCTGCCAGAT CCTCCGCTGG GCCCTCGCCC TGGGGCTGGG CCTCATGTTC 
GAGGTCACGC ACGCCTTCCG GTCTCAAGAT GAGTTCCTGT CCAGTCTGGA GAGCTATGAG
ATCGCCTTCC CCACCCGCGT GGACCACAAC GGGGCACTGC TGGCCTTCTC GCCACCTCCT
CCCCGGAGGC AGCGCCGCGG CACGGGGGCC ACAGCCGAGT CCCGCCTCTT CTACAAAGTG
GCCTCGCCCA GCACCCACTT CCTGCTGAAC CTGACCCGCA GCTCCCGTCT ACTGGCAGGG
CACGTCTCCG TGGAGTACTG GACACGGGAG GGCCTGGCCT GGCAGAGGGC GGCCCGGCCC
CACTGCCTCT ACGCTGGTCA CCTGCAGGGC CAGGCCAGCA GCTCCCATGT GGCCATCAGC
ACCTGTGGAG GCCTGCACGG CCTGATCGTG GCAGACGAGG AAGAGTACCT GATTGAGCCC
CTGCACGGTG GGCCCAAGGG TTCTCGGAGC CCGGAGGAAA GTGGACCACA TGTGGTGTAC
AAGCGTTCCT CTCTGCGTCA CCCCCACCTG GACACAGCCT GTGGAGTGAG AGATGAGAAA
CCGTGGAAAG GGCGGCCATG GTGGCTGCGG ACCTTGAAGC CACCGCCTGC CAGGCCCCTG
GGGAATGAAA CAGAGCGTGG CCAGCCAGGC CTGAAGCGAT CGGTCAGCCG AGAGCGCTAC
GTGGAGACCC TGGTGGTGGC TGACAAGATG ATGGTGGCCT ATCACGGGCG CCGGGATGTG
GAGCAGTATG TCCTGGCCAT CATGAACATT GTTGCCAAAC TTTTCCAGGA CTCGAGTCTG
GGAAGCACCG TTAACATCCT CGTAACTCGC CTCATCCTGC TCACGGAGGA CCAGCCCACT
CTGGAGATCA CCCACCATGC CGGGAAGTCC CTGGACAGCT TCTGTAAGTG GCAGAAATCC
ATCGTGAACC ACAGCGGCCA TGGCAATGCC ATTCCAGAGA ACGGTGTGGC TAACCATGAC
ACAGCAGTGC TCATCACACG CTATGACATC TGCATCTACA AGAACAAACC CTGCGGCACA
CTAGGCCTGG CCCCGGTGGG CGGAATGTGT GAGCGCGAGA GAAGCTGCAG CGTCAATGAG
GACATTGGCC TGGCCACAGC GTTCACCATT GCCCACGAGA TCGGGCACAC ATTCGGCATG
AACCATGACG GCGTGGGAAA CAGCTGTGGG GCCCGTGGTC AGGACCCAGC CAAGCTCATG
GCTGCCCACA TTACCATGAA GACCAACCCA TTCGTGTGGT CATCCTGCAG CCGTGACTAC
ATCACCAGCT TTCTAGACTC GGGCCTGGGG CTCTGCCTGA ACAACCGGCC CCCCAGACAG
GACTTTGTGT ACCCGACAGT GGCACCGGGC CAAGCCTACG ATGCAGATGA GCAATGCCGC
TTTCAGCATG GAGTCAAATC GCGTCAGTGT AAATACGGGG AGGTCTGCAG CGAGCTGTGG
TGTCTGAGCA AGAGCAACCG GTGCATCACC AACAGCATCC CGGCCGCCGA GGGCACGCTG
TGCCAGACGC ACACCATCGA CAAGGGGTGG TGCTACAAAC GGGTCTGTGT CCCCTTTGGG
TCGCGCCCAG AGGGTGTGGA CGGAGCCTGG GGGCCGTGGA CTCCATGGGG CGACTGCAGC
CGGACCTGTG GCGGCGGCGT GTCCTCTTCT AGCCGTCACT GCGACAGCCC CAGGCCAACC
ATCGGGGGCA AGTACTGTCT GGGTGAGAGA AGGCGGCACC GCTCCTGCAA CACGGATGAC
TGTCCCCCTG GCTCCCAGGA CTTCAGAGAA GTGCAGTGTT CTGAATTTGA CAGCATCCCT
TTCCGTGGGA AATTCTACAA GTGGAAAACG TACCGGGGAG GGGGCGTGAA GGCCTGCTCG
CTCACGTGCC TAGCGGAAGG CTTCAACTTC TACACGGAGA GGGCGGCAGC CGTGGTGGAC
GGGACACCCT GCCGTCCAGA CACGGTGGAC ATTTGCGTCA GTGGCGAATG CAAGCACGTG
GGCTGCGACC GAGTCCTGGG CTCCGACCTG CGGGAGGACA AGTGCCGAGT GTGTGGCGGT
GACGGCAGTG CCTGCGAGAC CATCGAGGGC GTCTTCAGCC CAGCCTCACC TGGGGCCGGG
TACGAGGATG TCGTCTGGAT TCCCAAAGGC TCCGTCCACA TCTTCATCCA GGATCTGAAC
CTCTCTCTCA GTCACTTGGC CCTGAAGGGA GACCAGGAGT CCCTGCTGCT GGAGGGGCTG
CCTGGGACCC CCCAGCCCCA CCGTCTGCCT CTAGCTGGGA CCACCTTTCA ACTGCGACAG
GGGCCAGACC AGGTCCAGAG CCTCGAAGCC CTGGGACCGA TTAATGCATC TCTCATCGTC
ATGGTGCTGG CCCGGACCGA GCTGCCTGCC CTCCGCTACC GCTTCAATGC CCCCATCGCC
CGTGACTCGC TGCCCCCCTA CTCCTGGCAC TATGCGCCCT GGACCAAGTG CTCGGCCCAG
TGTGCAGGCG GTAGCCAGGT GCAGGCGGTG GAGTGCCGCA ACCAGCTGGA CAGCTCCGCG
GTCGCCCCCC ACTACTGCAG TGCCCACAGC AAGCTGCCCA AAAGGCAGCG CGCCTGCAAC
ACGGAGCCTT GCCCTCCAGA CTGGGTTGTA GGGAACTGGT CGCTCTGCAG CCGCAGCTGC
GATGCAGGCG TGCGCAGCCG CTCGGTCGTG TGCCAGCGCC GCGTCTCTGC CGCGGAGGAG
AAGGCGCTGG ACGACAGCGC ATGCCCGCAG CCGCGCCCAC CTGTACTGGA GGCCTGCCAC
GGCCCCACTT GCCCTCCGGA GTGGGCGGCC CTCGACTGGT CTGAGTGCAC CCCCAGCTGC
GGGCCGGGCC TCCGCCACCG CGTGGTCCTT TGCAAGAGCG CAGACCACCG CGCCACGCTG
CCCCCGGCGC ACTGCTCACC CGCCGCCAAG CCACCGGCCA CCATGCGCTG CAACTTGCGC
CGCTGCCCCC CGGCCCGCTG GGTGGCTGGC GAGTGGGGTG AGTGCTCTGC ACAGTGCGGC
GTCGGGCAGC GGCAGCGCTC GGTGCGCTGC ACCAGCCACA CGGGCCAGGC GTCGCACGAG
TGCACGGAGG CCCTGCGGCC GCCCACCACG CAGCAGTGTG AGGCCAAGTG CGACAGCCCA
ACCCCCGGGG ACGGCCCTGA AGAGTGCAAG GATGTGAACA AGGTCGCCTA CTGCCCCCTG
GTGCTCAAAT TTCAGTTCTG CAGCCGAGCC TACTTCCGCC AGATGTGCTG CAAAACCTGC
CATGGCCACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20510
Clone ID Related Accession (Same CDS sequence) XM_017027338.1 , NM_030957.3
Accession Version XM_017027338.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCTCCCG CCTGCCAGAT CCTCCGCTGG GCCCTCGCCC TGGGGCTGGG CCTCATGTTC 
GAGGTCACGC ACGCCTTCCG GTCTCAAGAT GAGTTCCTGT CCAGTCTGGA GAGCTATGAG
ATCGCCTTCC CCACCCGCGT GGACCACAAC GGGGCACTGC TGGCCTTCTC GCCACCTCCT
CCCCGGAGGC AGCGCCGCGG CACGGGGGCC ACAGCCGAGT CCCGCCTCTT CTACAAAGTG
GCCTCGCCCA GCACCCACTT CCTGCTGAAC CTGACCCGCA GCTCCCGTCT ACTGGCAGGG
CACGTCTCCG TGGAGTACTG GACACGGGAG GGCCTGGCCT GGCAGAGGGC GGCCCGGCCC
CACTGCCTCT ACGCTGGTCA CCTGCAGGGC CAGGCCAGCA GCTCCCATGT GGCCATCAGC
ACCTGTGGAG GCCTGCACGG CCTGATCGTG GCAGACGAGG AAGAGTACCT GATTGAGCCC
CTGCACGGTG GGCCCAAGGG TTCTCGGAGC CCGGAGGAAA GTGGACCACA TGTGGTGTAC
AAGCGTTCCT CTCTGCGTCA CCCCCACCTG GACACAGCCT GTGGAGTGAG AGATGAGAAA
CCGTGGAAAG GGCGGCCATG GTGGCTGCGG ACCTTGAAGC CACCGCCTGC CAGGCCCCTG
GGGAATGAAA CAGAGCGTGG CCAGCCAGGC CTGAAGCGAT CGGTCAGCCG AGAGCGCTAC
GTGGAGACCC TGGTGGTGGC TGACAAGATG ATGGTGGCCT ATCACGGGCG CCGGGATGTG
GAGCAGTATG TCCTGGCCAT CATGAACATT GTTGCCAAAC TTTTCCAGGA CTCGAGTCTG
GGAAGCACCG TTAACATCCT CGTAACTCGC CTCATCCTGC TCACGGAGGA CCAGCCCACT
CTGGAGATCA CCCACCATGC CGGGAAGTCC CTGGACAGCT TCTGTAAGTG GCAGAAATCC
ATCGTGAACC ACAGCGGCCA TGGCAATGCC ATTCCAGAGA ACGGTGTGGC TAACCATGAC
ACAGCAGTGC TCATCACACG CTATGACATC TGCATCTACA AGAACAAACC CTGCGGCACA
CTAGGCCTGG CCCCGGTGGG CGGAATGTGT GAGCGCGAGA GAAGCTGCAG CGTCAATGAG
GACATTGGCC TGGCCACAGC GTTCACCATT GCCCACGAGA TCGGGCACAC ATTCGGCATG
AACCATGACG GCGTGGGAAA CAGCTGTGGG GCCCGTGGTC AGGACCCAGC CAAGCTCATG
GCTGCCCACA TTACCATGAA GACCAACCCA TTCGTGTGGT CATCCTGCAG CCGTGACTAC
ATCACCAGCT TTCTAGACTC GGGCCTGGGG CTCTGCCTGA ACAACCGGCC CCCCAGACAG
GACTTTGTGT ACCCGACAGT GGCACCGGGC CAAGCCTACG ATGCAGATGA GCAATGCCGC
TTTCAGCATG GAGTCAAATC GCGTCAGTGT AAATACGGGG AGGTCTGCAG CGAGCTGTGG
TGTCTGAGCA AGAGCAACCG GTGCATCACC AACAGCATCC CGGCCGCCGA GGGCACGCTG
TGCCAGACGC ACACCATCGA CAAGGGGTGG TGCTACAAAC GGGTCTGTGT CCCCTTTGGG
TCGCGCCCAG AGGGTGTGGA CGGAGCCTGG GGGCCGTGGA CTCCATGGGG CGACTGCAGC
CGGACCTGTG GCGGCGGCGT GTCCTCTTCT AGCCGTCACT GCGACAGCCC CAGGCCAACC
ATCGGGGGCA AGTACTGTCT GGGTGAGAGA AGGCGGCACC GCTCCTGCAA CACGGATGAC
TGTCCCCCTG GCTCCCAGGA CTTCAGAGAA GTGCAGTGTT CTGAATTTGA CAGCATCCCT
TTCCGTGGGA AATTCTACAA GTGGAAAACG TACCGGGGAG GGGGCGTGAA GGCCTGCTCG
CTCACGTGCC TAGCGGAAGG CTTCAACTTC TACACGGAGA GGGCGGCAGC CGTGGTGGAC
GGGACACCCT GCCGTCCAGA CACGGTGGAC ATTTGCGTCA GTGGCGAATG CAAGCACGTG
GGCTGCGACC GAGTCCTGGG CTCCGACCTG CGGGAGGACA AGTGCCGAGT GTGTGGCGGT
GACGGCAGTG CCTGCGAGAC CATCGAGGGC GTCTTCAGCC CAGCCTCACC TGGGGCCGGG
TACGAGGATG TCGTCTGGAT TCCCAAAGGC TCCGTCCACA TCTTCATCCA GGATCTGAAC
CTCTCTCTCA GTCACTTGGC CCTGAAGGGA GACCAGGAGT CCCTGCTGCT GGAGGGGCTG
CCTGGGACCC CCCAGCCCCA CCGTCTGCCT CTAGCTGGGA CCACCTTTCA ACTGCGACAG
GGGCCAGACC AGGTCCAGAG CCTCGAAGCC CTGGGACCGA TTAATGCATC TCTCATCGTC
ATGGTGCTGG CCCGGACCGA GCTGCCTGCC CTCCGCTACC GCTTCAATGC CCCCATCGCC
CGTGACTCGC TGCCCCCCTA CTCCTGGCAC TATGCGCCCT GGACCAAGTG CTCGGCCCAG
TGTGCAGGCG GTAGCCAGGT GCAGGCGGTG GAGTGCCGCA ACCAGCTGGA CAGCTCCGCG
GTCGCCCCCC ACTACTGCAG TGCCCACAGC AAGCTGCCCA AAAGGCAGCG CGCCTGCAAC
ACGGAGCCTT GCCCTCCAGA CTGGGTTGTA GGGAACTGGT CGCTCTGCAG CCGCAGCTGC
GATGCAGGCG TGCGCAGCCG CTCGGTCGTG TGCCAGCGCC GCGTCTCTGC CGCGGAGGAG
AAGGCGCTGG ACGACAGCGC ATGCCCGCAG CCGCGCCCAC CTGTACTGGA GGCCTGCCAC
GGCCCCACTT GCCCTCCGGA GTGGGCGGCC CTCGACTGGT CTGAGTGCAC CCCCAGCTGC
GGGCCGGGCC TCCGCCACCG CGTGGTCCTT TGCAAGAGCG CAGACCACCG CGCCACGCTG
CCCCCGGCGC ACTGCTCACC CGCCGCCAAG CCACCGGCCA CCATGCGCTG CAACTTGCGC
CGCTGCCCCC CGGCCCGCTG GGTGGCTGGC GAGTGGGGTG AGTGCTCTGC ACAGTGCGGC
GTCGGGCAGC GGCAGCGCTC GGTGCGCTGC ACCAGCCACA CGGGCCAGGC GTCGCACGAG
TGCACGGAGG CCCTGCGGCC GCCCACCACG CAGCAGTGTG AGGCCAAGTG CGACAGCCCA
ACCCCCGGGG ACGGCCCTGA AGAGTGCAAG GATGTGAACA AGGTCGCCTA CTGCCCCCTG
GTGCTCAAAT TTCAGTTCTG CAGCCGAGCC TACTTCCGCC AGATGTGCTG CAAAACCTGC
CATGGCCACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016882827.1
CDS163..3474
Misc Feature(1)379..699
Misc Feature(2)877..1533
Misc Feature(3)877..1524
Misc Feature(4)order(1336..1341,1348..1350,1366..1368)
Misc Feature(5)1810..1968
Misc Feature(6)2278..2616
Misc Feature(7)2647..2817
Misc Feature(8)3016..3171
Misc Feature(9)3190..3324
Misc Feature(10)3367..3462
Translation

Target ORF information:

RefSeq Version XM_017027338.1
Organism Homo sapiens(Human)
Definition Homo sapiens ADAM metallopeptidase with thrombospondin type 1 motif 10 (ADAMTS10), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017027338.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
ATGGCTCCCG CCTGCCAGAT CCTCCGCTGG GCCCTCGCCC TGGGGCTGGG CCTCATGTTC 
GAGGTCACGC ACGCCTTCCG GTCTCAAGAT GAGTTCCTGT CCAGTCTGGA GAGCTATGAG
ATCGCCTTCC CCACCCGCGT GGACCACAAC GGGGCACTGC TGGCCTTCTC GCCACCTCCT
CCCCGGAGGC AGCGCCGCGG CACGGGGGCC ACAGCCGAGT CCCGCCTCTT CTACAAAGTG
GCCTCGCCCA GCACCCACTT CCTGCTGAAC CTGACCCGCA GCTCCCGTCT ACTGGCAGGG
CACGTCTCCG TGGAGTACTG GACACGGGAG GGCCTGGCCT GGCAGAGGGC GGCCCGGCCC
CACTGCCTCT ACGCTGGTCA CCTGCAGGGC CAGGCCAGCA GCTCCCATGT GGCCATCAGC
ACCTGTGGAG GCCTGCACGG CCTGATCGTG GCAGACGAGG AAGAGTACCT GATTGAGCCC
CTGCACGGTG GGCCCAAGGG TTCTCGGAGC CCGGAGGAAA GTGGACCACA TGTGGTGTAC
AAGCGTTCCT CTCTGCGTCA CCCCCACCTG GACACAGCCT GTGGAGTGAG AGATGAGAAA
CCGTGGAAAG GGCGGCCATG GTGGCTGCGG ACCTTGAAGC CACCGCCTGC CAGGCCCCTG
GGGAATGAAA CAGAGCGTGG CCAGCCAGGC CTGAAGCGAT CGGTCAGCCG AGAGCGCTAC
GTGGAGACCC TGGTGGTGGC TGACAAGATG ATGGTGGCCT ATCACGGGCG CCGGGATGTG
GAGCAGTATG TCCTGGCCAT CATGAACATT GTTGCCAAAC TTTTCCAGGA CTCGAGTCTG
GGAAGCACCG TTAACATCCT CGTAACTCGC CTCATCCTGC TCACGGAGGA CCAGCCCACT
CTGGAGATCA CCCACCATGC CGGGAAGTCC CTGGACAGCT TCTGTAAGTG GCAGAAATCC
ATCGTGAACC ACAGCGGCCA TGGCAATGCC ATTCCAGAGA ACGGTGTGGC TAACCATGAC
ACAGCAGTGC TCATCACACG CTATGACATC TGCATCTACA AGAACAAACC CTGCGGCACA
CTAGGCCTGG CCCCGGTGGG CGGAATGTGT GAGCGCGAGA GAAGCTGCAG CGTCAATGAG
GACATTGGCC TGGCCACAGC GTTCACCATT GCCCACGAGA TCGGGCACAC ATTCGGCATG
AACCATGACG GCGTGGGAAA CAGCTGTGGG GCCCGTGGTC AGGACCCAGC CAAGCTCATG
GCTGCCCACA TTACCATGAA GACCAACCCA TTCGTGTGGT CATCCTGCAG CCGTGACTAC
ATCACCAGCT TTCTAGACTC GGGCCTGGGG CTCTGCCTGA ACAACCGGCC CCCCAGACAG
GACTTTGTGT ACCCGACAGT GGCACCGGGC CAAGCCTACG ATGCAGATGA GCAATGCCGC
TTTCAGCATG GAGTCAAATC GCGTCAGTGT AAATACGGGG AGGTCTGCAG CGAGCTGTGG
TGTCTGAGCA AGAGCAACCG GTGCATCACC AACAGCATCC CGGCCGCCGA GGGCACGCTG
TGCCAGACGC ACACCATCGA CAAGGGGTGG TGCTACAAAC GGGTCTGTGT CCCCTTTGGG
TCGCGCCCAG AGGGTGTGGA CGGAGCCTGG GGGCCGTGGA CTCCATGGGG CGACTGCAGC
CGGACCTGTG GCGGCGGCGT GTCCTCTTCT AGCCGTCACT GCGACAGCCC CAGGCCAACC
ATCGGGGGCA AGTACTGTCT GGGTGAGAGA AGGCGGCACC GCTCCTGCAA CACGGATGAC
TGTCCCCCTG GCTCCCAGGA CTTCAGAGAA GTGCAGTGTT CTGAATTTGA CAGCATCCCT
TTCCGTGGGA AATTCTACAA GTGGAAAACG TACCGGGGAG GGGGCGTGAA GGCCTGCTCG
CTCACGTGCC TAGCGGAAGG CTTCAACTTC TACACGGAGA GGGCGGCAGC CGTGGTGGAC
GGGACACCCT GCCGTCCAGA CACGGTGGAC ATTTGCGTCA GTGGCGAATG CAAGCACGTG
GGCTGCGACC GAGTCCTGGG CTCCGACCTG CGGGAGGACA AGTGCCGAGT GTGTGGCGGT
GACGGCAGTG CCTGCGAGAC CATCGAGGGC GTCTTCAGCC CAGCCTCACC TGGGGCCGGG
TACGAGGATG TCGTCTGGAT TCCCAAAGGC TCCGTCCACA TCTTCATCCA GGATCTGAAC
CTCTCTCTCA GTCACTTGGC CCTGAAGGGA GACCAGGAGT CCCTGCTGCT GGAGGGGCTG
CCTGGGACCC CCCAGCCCCA CCGTCTGCCT CTAGCTGGGA CCACCTTTCA ACTGCGACAG
GGGCCAGACC AGGTCCAGAG CCTCGAAGCC CTGGGACCGA TTAATGCATC TCTCATCGTC
ATGGTGCTGG CCCGGACCGA GCTGCCTGCC CTCCGCTACC GCTTCAATGC CCCCATCGCC
CGTGACTCGC TGCCCCCCTA CTCCTGGCAC TATGCGCCCT GGACCAAGTG CTCGGCCCAG
TGTGCAGGCG GTAGCCAGGT GCAGGCGGTG GAGTGCCGCA ACCAGCTGGA CAGCTCCGCG
GTCGCCCCCC ACTACTGCAG TGCCCACAGC AAGCTGCCCA AAAGGCAGCG CGCCTGCAAC
ACGGAGCCTT GCCCTCCAGA CTGGGTTGTA GGGAACTGGT CGCTCTGCAG CCGCAGCTGC
GATGCAGGCG TGCGCAGCCG CTCGGTCGTG TGCCAGCGCC GCGTCTCTGC CGCGGAGGAG
AAGGCGCTGG ACGACAGCGC ATGCCCGCAG CCGCGCCCAC CTGTACTGGA GGCCTGCCAC
GGCCCCACTT GCCCTCCGGA GTGGGCGGCC CTCGACTGGT CTGAGTGCAC CCCCAGCTGC
GGGCCGGGCC TCCGCCACCG CGTGGTCCTT TGCAAGAGCG CAGACCACCG CGCCACGCTG
CCCCCGGCGC ACTGCTCACC CGCCGCCAAG CCACCGGCCA CCATGCGCTG CAACTTGCGC
CGCTGCCCCC CGGCCCGCTG GGTGGCTGGC GAGTGGGGTG AGTGCTCTGC ACAGTGCGGC
GTCGGGCAGC GGCAGCGCTC GGTGCGCTGC ACCAGCCACA CGGGCCAGGC GTCGCACGAG
TGCACGGAGG CCCTGCGGCC GCCCACCACG CAGCAGTGTG AGGCCAAGTG CGACAGCCCA
ACCCCCGGGG ACGGCCCTGA AGAGTGCAAG GATGTGAACA AGGTCGCCTA CTGCCCCCTG
GTGCTCAAAT TTCAGTTCTG CAGCCGAGCC TACTTCCGCC AGATGTGCTG CAAAACCTGC
CATGGCCACT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Screening ADAMTS10 in dog populations supports Gly661Arg as the glaucoma-causing variant in beagles.
Investigative ophthalmology & visual science54(3)1881-6(2013 Mar)
Kuchtey J,Kunkel J,Esson D,Sapienza JS,Ward DA,Plummer CE,Gelatt KN,Kuchtey RW


book

SuperTAG methylation-specific digital karyotyping reveals uremia-induced epigenetic dysregulation of atherosclerosis-related genes.
Circulation. Cardiovascular genetics5(6)611-20(2012 Dec)
Zawada AM,Rogacev KS,Hummel B,Grün OS,Friedrich A,Rotter B,Winter P,Geisel J,Fliser D,Heine GH


book

ADAMTS10 protein interacts with fibrillin-1 and promotes its deposition in extracellular matrix of cultured fibroblasts.
The Journal of biological chemistry286(19)17156-67(2011 May)
Kutz WE,Wang LW,Bader HL,Majors AK,Iwata K,Traboulsi EI,Sakai LY,Keene DR,Apte SS


book

Homozygous mutations in ADAMTS10 and ADAMTS17 cause lenticular myopia, ectopia lentis, glaucoma, spherophakia, and short stature.
American journal of human genetics85(5)558-68(2009 Nov)
Morales J,Al-Sharif L,Khalil DS,Shinwari JM,Bavi P,Al-Mahrouqi RA,Al-Rajhi A,Alkuraya FS,Meyer BF,Al Tassan N


 
*
*
*
*