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HIST1H4I cDNA ORF clone, Homo sapiens (human)

Gene Symbol HIST1H4I
Entrez Gene ID 8294
Full Name histone cluster 1, H4i
Synonyms H4/m, H4FM, H4M
General protein information
Preferred Names
histone H4
Names
histone H4
histone 1, H4i
histone family member
Histone 4 family, member M
H4 histone family, member M
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

6

6p21.33

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]. lac of sum
Disorder MIM:

602833


mRNA and Protein(s)

mRNA Protein Name
NM_003495 NP_003486 histone H4



Homo sapiens (human) HIST1H4I NP_003486.1
Pan troglodytes (chimpanzee) LOC748545 XP_001172771.2
Macaca mulatta (Rhesus monkey) LOC695323 XP_001083839.1
Bos taurus (cattle) LOC527645 XP_002697516.1
Mus musculus (house mouse) Hist1h4h NP_694813.1
Rattus norvegicus (Norway rat) Hist1h4a XP_006254042.1
Gallus gallus (chicken) HIST1H46L4 XP_003640418.1
Danio rerio (zebrafish) LOC794549 XP_005172451.1
Drosophila melanogaster (fruit fly) His4:CG33885 NP_001027342.1
Caenorhabditis elegans his-14 NP_496889.1
Arabidopsis thaliana (thale cress) HIS4 NP_180441.1
Xenopus (Silurana) tropicalis (western clawed frog) LOC101731642 XP_004919466.1

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HIST1H4I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H4I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu30070
NM_003495 Homo sapiens histone cluster 1, H4i (HIST1H4I), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30070
Clone ID Related Accession (Same CDS sequence) NM_003495
Accession Version NM_003495.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 312bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 18-AUG-2015
Organism Homo sapiens (human)
Product histone H4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB000905.1. On Jan 10, 2002 this sequence version replaced gi:4504320. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGTCAGGAC GCGGCAAAGG AGGTAAGGGC CTGGGGAAAG GGGGTGCCAA GCGCCACCGC 
AAGGTGCTGC GCGACAACAT CCAGGGTATC ACCAAGCCAG CCATTCGGCG CCTTGCTCGC
CGCGGCGGCG TGAAGCGCAT TTCTGGCCTC ATCTATGAGG AGACCCGCGG AGTGTTGAAG
GTGTTCCTGG AGAACGTGAT CCGGGACGCC GTGACCTACA CGGAGCACGC CAAGCGCAAG
ACGGTCACCG CCATGGACGT GGTCTACGCG CTCAAGCGCC AGGGCCGCAC CCTCTATGGC
TTCGGCGGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003486.1
CDS1..312
Misc Feature(1)1..309(+)
Misc Feature(2)4..6(+)
Misc Feature(3)4..6(+)
Misc Feature(4)10..12(+)
Misc Feature(5)10..12(+)
Misc Feature(6)16..18(+)
Misc Feature(7)16..18(+)
Misc Feature(8)25..27(+)
Misc Feature(9)25..27(+)
Misc Feature(10)37..39(+)
Misc Feature(11)37..39(+)
Misc Feature(12)49..51(+)
Misc Feature(13)49..51(+)
Misc Feature(14)52..303(+)
Misc Feature(15)61..63(+)
Misc Feature(16)94..96(+)
Misc Feature(17)100..273(+)
Misc Feature(18)121..135(+)
Misc Feature(19)133..291(+)
Misc Feature(20)142..144(+)
Misc Feature(21)154..156(+)
Misc Feature(22)205..279(+)
Misc Feature(23)265..267(+)
Misc Feature(24)274..276(+)
Misc Feature(25)274..276(+)
Misc Feature(26)289..303(+)
Exon (1)1..370
Gene:HIST1H4I
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003495
Organism Homo sapiens (human)
Definition Homo sapiens histone cluster 1, H4i (HIST1H4I), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003495

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCAGGAC GCGGCAAAGG AGGTAAGGGC CTGGGGAAAG GGGGTGCCAA GCGCCACCGC 
AAGGTGCTGC GCGACAACAT CCAGGGTATC ACCAAGCCAG CCATTCGGCG CCTTGCTCGC
CGCGGCGGCG TGAAGCGCAT TTCTGGCCTC ATCTATGAGG AGACCCGCGG AGTGTTGAAG
GTGTTCCTGG AGAACGTGAT CCGGGACGCC GTGACCTACA CGGAGCACGC CAAGCGCAAG
ACGGTCACCG CCATGGACGT GGTCTACGCG CTCAAGCGCC AGGGCCGCAC CCTCTATGGC
TTCGGCGGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Human tNASP promotes in vitro nucleosome assembly with histone H3.3
Biochemistry 54 (5), 1171-1179 (2015)
Kato D, Osakabe A, Tachiwana H, Tanaka H and Kurumizaka H.


book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

Structural insights into recognition of acetylated histone ligands by the BRPF1 bromodomain
FEBS Lett. 588 (21), 3844-3854 (2014)
Lubula MY, Eckenroth BE, Carlson S, Poplawski A, Chruszcz M and Glass KC.


book

Molecular functions of the TLE tetramerization domain in Wnt target gene repression
EMBO J. 33 (7), 719-731 (2014)
Chodaparambil JV, Pate KT, Hepler MR, Tsai BP, Muthurajan UM, Luger K, Waterman ML and Weis WI.


book

Structure of human nucleosome containing the testis-specific histone variant TSH2B
Acta Crystallogr F Struct Biol Commun 70 (PT 4), 444-449 (2014)
Urahama T, Horikoshi N, Osakabe A, Tachiwana H and Kurumizaka H.


book

The human and mouse replication-dependent histone genes
Genomics 80 (5), 487-498 (2002)
Marzluff WF, Gongidi P, Woods KR, Jin J and Maltais LJ.


book

Isolation and characterization of two human H1 histone genes within clusters of core histone genes
Genomics 10 (4), 940-948 (1991)
Albig W, Kardalinou E, Drabent B, Zimmer A and Doenecke D.


book

Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies
FEBS Lett. 253 (1-2), 141-145 (1989)
Turner BM, O'Neill LP and Allan IM.


book

A highly basic histone H4 domain bound to the sharply bent region of nucleosomal DNA
Nature 331 (6154), 365-367 (1988)
Ebralidse KK, Grachev SA and Mirzabekov AD.


book

Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene
Science 236 (4806), 1308-1311 (1987)
Pauli,U., Chrysogelos,S., Stein,G., Stein,J. and Nick,H.


 
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