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CARS1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol CARS1
Entrez Gene ID 833
Full Name cysteinyl-tRNA synthetase
Synonyms CARS1,CYSRS,MGC:11246
General protein information
Preferred Names

cysteinyl-tRNA synthetase

Names

cysteine--tRNA ligase, cytoplasmic
cysteine tRNA ligase 1, cytoplasmic
cysteine translase
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

11

11p15.4
Summary This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010].
Disorder MIM:

123859


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006718341.3 XP_006718404.1 cysteine--tRNA ligase, cytoplasmic isoform X5
XM_017018391.1 XP_016873880.1 cysteine--tRNA ligase, cytoplasmic isoform X8
NM_001014437.3 NP_001014437.1 cysteine--tRNA ligase, cytoplasmic isoform c
NM_001194997.2 NP_001181926.1 cysteine--tRNA ligase, cytoplasmic isoform e
NM_001751.5 NP_001742.1 cysteine--tRNA ligase, cytoplasmic isoform b
NM_139273.3 NP_644802.1 cysteine--tRNA ligase, cytoplasmic isoform a
XM_017018392.2 XP_016873881.1 cysteine--tRNA ligase, cytoplasmic isoform X9
XM_017018389.1 XP_016873878.1 cysteine--tRNA ligase, cytoplasmic isoform X6
XM_017018390.1 XP_016873879.1 cysteine--tRNA ligase, cytoplasmic isoform X6
NM_001194997.1 NP_001181926.1 cysteine--tRNA ligase, cytoplasmic isoform e
NM_001014437.2 NP_001014437.1 cysteine--tRNA ligase, cytoplasmic isoform c
XM_017018392.1 XP_016873881.1 cysteine--tRNA ligase, cytoplasmic isoform X4
XM_024448701.1 XP_024304469.1 cysteine--tRNA ligase, cytoplasmic isoform X1
XM_024448702.1 XP_024304470.1 cysteine--tRNA ligase, cytoplasmic isoform X2
XM_024448703.1 XP_024304471.1 cysteine--tRNA ligase, cytoplasmic isoform X3
XM_024448704.1 XP_024304472.1 cysteine--tRNA ligase, cytoplasmic isoform X4
XM_024448705.1 XP_024304473.1 cysteine--tRNA ligase, cytoplasmic isoform X7
NM_139273.4 NP_644802.1 cysteine--tRNA ligase, cytoplasmic isoform a
NM_001751.6 NP_001742.1 cysteine--tRNA ligase, cytoplasmic isoform b

Pathways from BioSystems


Homologies


CARS XP_001096302.1
Oryza sativa (rice) Os09g0556500 NP_001063899.1
Homo sapiens (human) CARS NP_001014437.1
Rattus norvegicus (Norway rat) Cars NP_001099789.1
Drosophila melanogaster (fruit fly) Aats-cys NP_611087.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP012283 XP_320253.2
Arabidopsis thaliana (thale cress) SYCO ARATH NP_565717.2
Mus musculus (house mouse) Cars NP_038770.3
Gallus gallus (chicken) CARS NP_001012601.1
Caenorhabditis elegans (roundworm) cars-1 NP_491014.1
Saccharomyces cerevisiae (baker's yeast) YNL247W NP_014152.1
Kluyveromyces lactis KLLA0C03025g XP_452332.1
Pan troglodytes (chimpanzee) CARS XP_003312912.1
Canis lupus familiaris (dog) CARS XP_851224.1
Bos taurus (cattle) CARS NP_001096719.1
Xenopus tropicalis (tropical clawed frog) cars NP_001011365.1
Danio rerio (zebrafish) cars NP_001112372.1
Schizosaccharomyces pombe (fission yeast) SPAC29E6.06c NP_594564.1

Gene Ontology


Bibliography

Related articles in PubMed

Polymorphisms in CARS are associated with gastric cancer risk: a two-stage case-control study in the Chinese population.
Tian T, Xiao L, Du J, Zhu X, Gu Y, Qin N, Yan C, Liu L, Ma H, Jiang Y, Chen J, Yu H, Dai J
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association20(6)940-947(2017 Nov)

Loss of cysteinyl-tRNA synthetase (CARS) induces the transsulfuration pathway and inhibits ferroptosis induced by cystine deprivation.
Hayano M, Yang WS, Corn CK, Pagano NC, Stockwell BR
Cell death and differentiation23(2)270-8(2016 Feb)

Evaluation of associations between single nucleotide polymorphisms in the FRMD3 and CARS genes and diabetic nephropathy in a Kuwaiti population.
Al-waheeb S, Alwohhaib M, Abdelghani A, Al-Sharrah S, Al-Shafey E, Al-Sahow A, Alyatama N, Dashti AA, Hussain N
Genetics and molecular research : GMR15(1)(2016 Jan)

Meta-analysis of genome-wide association studies identifies six new Loci for serum calcium concentrations.
O'Seaghdha CM, Wu H, Yang Q, Kapur K, Guessous I, Zuber AM, K?ttgen A, Stoudmann C, Teumer A, Kutalik Z, Mangino M, Dehghan A, Zhang W, Eiriksdottir G, Li G, Tanaka T, Portas L, Lopez LM, Hayward C, Lohman K, Matsuda K, Padmanabhan S, Firsov D, Sorice R, Ulivi S, Brockhaus AC, Kleber ME, Mahajan A, Ernst FD, Gudnason V, Launer LJ, Mace A, Boerwinckle E, Arking DE, Tanikawa C, Nakamura Y, Brown MJ, Gaspoz JM, Theler JM, Siscovick DS, Psaty BM, Bergmann S, Vollenweider P, Vitart V, Wright AF, Zemunik T, Boban M, Kolcic I, Navarro P, Brown EM, Estrada K, Ding J, Harris TB, Bandinelli S, Hernandez D, Singleton AB, Girotto G, Ruggiero D, d'Adamo AP, Robino A, Meitinger T, Meisinger C, Davies G, Starr JM, Chambers JC, Boehm BO, Winkelmann BR, Huang J, Murgia F, Wild SH, Campbell H, Morris AP, Franco OH, Hofman A, Uitterlinden AG, Rivadeneira F, V?lker U, Hannemann A, Biffar R, Hoffmann W, Shin SY, Lescuyer P, Henry H, Schurmann C, , , Munroe PB, Gasparini P, Pirastu N, Ciullo M, Gieger C, M?rz W, Lind L, Spector TD, Smith AV, Rudan I, Wilson JF, Polasek O, Deary IJ, Pirastu M, Ferrucci L, Liu Y, Kestenbaum B, Kooner JS, Witteman JC, Nauck M, Kao WH, Wallaschofski H, Bonny O, Fox CS, Bochud M
PLoS genetics9(9)e1003796(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of CARS, the cysteinyl-tRNA synthetase, suppresses ferroptosis induced by erastin, which inhibits the cystine-glutamate antiporter known as system xc(-).
Title: Loss of cysteinyl-tRNA synthetase (CARS) induces the transsulfuration pathway and inhibits ferroptosis induced by cystine deprivation.

The risk (G and C) and non-risk (C and T) allele frequencies of the SNPs at the rs1888747 and rs739401 loci of FRMD3 and CARS, respectively, did not differ significantly between the diabetics with (case) and without (control) nephropathy (P > 0.05).
Title: Evaluation of associations between single nucleotide polymorphisms in the FRMD3 and CARS genes and diabetic nephropathy in a Kuwaiti population.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: A three-stage genome-wide association study of general cognitive ability: hunting the small effects.

Observational study of gene-disease association. (HuGE Navigator)
Title: Replication study for the association between four Loci identified by a genome-wide association study on European American subjects with type 1 diabetes and susceptibility to diabetic nephropathy in Japanese subjects with type 2 diabetes.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Genome-wide association study of alcohol dependence implicates a region on chromosome 11.

The following CARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu13862 NM_001751.6
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu92568 XM_017018391.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 10-12 $307.30
$439.00
OHu13803 NM_139273.4
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu13978 NM_001014437.3
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $587.30
$839.00
OHu14615 NM_001194997.2
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $573.30
$819.00
OHu45024 XM_006718341.3
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu103622 XM_024448701.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu103623 XM_024448702.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu103624 XM_024448703.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu103625 XM_024448704.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu103626 XM_024448705.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu92567 XM_017018389.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu92567 XM_017018390.1
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu92569 XM_017018392.2
Latest version!		 Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X18, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu14615 NM_001194997.1 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $573.30
$819.00
OHu13978 NM_001014437.2 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $587.30
$839.00
OHu92569 XM_017018392.1 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu13803 NM_139273.3 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu13862 NM_001751.5 Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu13862
Clone ID Related Accession (Same CDS sequence) NM_001751.5 , NM_001751.6
Accession Version NM_001751.5 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-22
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform b
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, BC002880.2 and AI432096.1. On Aug 17, 2010 this sequence version replaced NM_001751.4. Transcript Variant: This variant (2) uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a longer and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002880.2, SRR1803617.20540.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

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ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA 
GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG 
ATGGCCCAGA ATGGAAGCTT CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001742.1
CDS85..2331
Translation

Target ORF information:

RefSeq Version NM_001751.5
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001751.5

ORF Insert Sequence:

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121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA 
GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG 
ATGGCCCAGA ATGGAAGCTT CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13862
Clone ID Related Accession (Same CDS sequence) NM_001751.5 , NM_001751.6
Accession Version NM_001751.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform b
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002880.2. On Jun 2, 2019 this sequence version replaced NM_001751.5. Transcript Variant: This variant (2) uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a longer and distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1105396.1, SRR3476690.587542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA 
GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG 
ATGGCCCAGA ATGGAAGCTT CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001742.1
CDS57..2303
Translation

Target ORF information:

RefSeq Version NM_001751.6
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001751.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGGTCTGCC CACACATGAC ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA 
GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT CAGGAGAAGC TCTACAAGGA ATATCTGCAG 
ATGGCCCAGA ATGGAAGCTT CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92568
Clone ID Related Accession (Same CDS sequence) XM_017018391.1
Accession Version XM_017018391.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X8
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC 
CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC 
GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC 
AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT 
TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC 
TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT 
CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC 
CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA 
GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA 
GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT 
GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC 
TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA 
GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG 
TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA 
AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG CACGGCAGTT GCGGCTGGCC 
TTCCTCATGC ACTCGTGGAA GGACACCCTG GACTACTCCA GCAACACCAT GGAGTCAGCG 
CTTCAATATG AGAAGTTCTT GAATGAGTTT TTCTTAAATG TGAAAGATAT CCTTCGCGCT 
CCTGTTGACA TCACTGGTCA GTTTGAGAAG TGGGGAGAAG AAGAAGCAGA ACTGAATAAG 
AACTTTTATG ACAAGAAGAC AGCAATTCAC AAAGCCCTCT GTGACAATGT TGACACCCGC 
ACCGTCATGG AAGAGATGCG GGCCTTGGTC AGTCAGTGCA ACCTCTATAT GGCAGCCCGG 
AAAGCCGTGA GGAAGAGGCC CAACCAGGCT CTGCTGGAGA ACATCGCCCT GTACCTCACC 
CATATGCTGA AGATCTTTGG GGCCGTAGAA GAGGACAGCT CCCTGGGATT CCCGGTCGGA 
GGGCCTGGAA CCAGCCTCAG TCTCGAGGCC ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCCGAG AAGGAGTGCG GAAGATTGCC CGAGAGCAAA AAGTCCCTGA GATTCTGCAG 
CTCAGCGATG CCCTGCGGGA CAACATCCTG CCCGAGCTTG GGGTGCGGTT TGAAGACCAC 
GAAGGACTGC CCACAGTGGT GAAACTGGTA GACAGAAACA CCTTATTAAA AGAGAGAGAA 
GAAAAGAGAC GGGTTGAAGA GGAGAAGAGG AAGAAGAAAG AGGAGGCGGC CCGGAGGAAA 
CAGGAACAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATTC CCCCCAGTGA GATGTTCTTG 
TCAGAAACCG ACAAATACTC CAAGTTTGAT GAAAATGGTC TGCCCACACA TGACATGGAG 
GGCAAAGAGC TCAGCAAAGG GCAAGCCAAG AAGCTGAAGA AGCTCTTCGA GGCTCAGGAG 
AAGCTCTACA AGGAATATCT GCAGATGGCC CAGAATGGAA GCTTCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873880.1
CDS608..2578
Translation

Target ORF information:

RefSeq Version XM_017018391.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017018391.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC 
CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC 
GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC 
AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT 
TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC 
TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT 
CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC 
CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA 
GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA 
GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT 
GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC 
TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA 
GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG 
TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA 
AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG CACGGCAGTT GCGGCTGGCC 
TTCCTCATGC ACTCGTGGAA GGACACCCTG GACTACTCCA GCAACACCAT GGAGTCAGCG 
CTTCAATATG AGAAGTTCTT GAATGAGTTT TTCTTAAATG TGAAAGATAT CCTTCGCGCT 
CCTGTTGACA TCACTGGTCA GTTTGAGAAG TGGGGAGAAG AAGAAGCAGA ACTGAATAAG 
AACTTTTATG ACAAGAAGAC AGCAATTCAC AAAGCCCTCT GTGACAATGT TGACACCCGC 
ACCGTCATGG AAGAGATGCG GGCCTTGGTC AGTCAGTGCA ACCTCTATAT GGCAGCCCGG 
AAAGCCGTGA GGAAGAGGCC CAACCAGGCT CTGCTGGAGA ACATCGCCCT GTACCTCACC 
CATATGCTGA AGATCTTTGG GGCCGTAGAA GAGGACAGCT CCCTGGGATT CCCGGTCGGA 
GGGCCTGGAA CCAGCCTCAG TCTCGAGGCC ACAGTCATGC CCTACCTTCA GGTGTTATCA 
GAATTCCGAG AAGGAGTGCG GAAGATTGCC CGAGAGCAAA AAGTCCCTGA GATTCTGCAG 
CTCAGCGATG CCCTGCGGGA CAACATCCTG CCCGAGCTTG GGGTGCGGTT TGAAGACCAC 
GAAGGACTGC CCACAGTGGT GAAACTGGTA GACAGAAACA CCTTATTAAA AGAGAGAGAA 
GAAAAGAGAC GGGTTGAAGA GGAGAAGAGG AAGAAGAAAG AGGAGGCGGC CCGGAGGAAA 
CAGGAACAAG AAGCAGCAAA GCTGGCCAAG ATGAAGATTC CCCCCAGTGA GATGTTCTTG 
TCAGAAACCG ACAAATACTC CAAGTTTGAT GAAAATGGTC TGCCCACACA TGACATGGAG 
GGCAAAGAGC TCAGCAAAGG GCAAGCCAAG AAGCTGAAGA AGCTCTTCGA GGCTCAGGAG 
AAGCTCTACA AGGAATATCT GCAGATGGCC CAGAATGGAA GCTTCCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13803
Clone ID Related Accession (Same CDS sequence) NM_139273.3 , NM_139273.4
Accession Version NM_139273.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-21
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AF288207.1, AI608729.1 and AI432096.1. On Aug 17, 2010 this sequence version replaced NM_139273.2. Transcript Variant: This variant (1) consists of 22 exons and encodes the shortest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.233531.1, SRR1803615.210092.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA 
AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_644802.1
CDS85..2265
Translation

Target ORF information:

RefSeq Version NM_139273.3
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_139273.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA 
AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13803
Clone ID Related Accession (Same CDS sequence) NM_139273.3 , NM_139273.4
Accession Version NM_139273.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform a
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AF288207.1 and AI608729.1. On Jun 2, 2019 this sequence version replaced NM_139273.3. Transcript Variant: This variant (1) consists of 22 exons and encodes the shortest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.269303.1, SRR1803613.181635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA 
AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_644802.1
CDS57..2237
Translation

Target ORF information:

RefSeq Version NM_139273.4
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_139273.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGCACG GCAGTTGCGG CTGGCCTTCC TCATGCACTC GTGGAAGGAC 
ACCCTGGACT ACTCCAGCAA CACCATGGAG TCAGCGCTTC AATATGAGAA GTTCTTGAAT 
GAGTTTTTCT TAAATGTGAA AGATATCCTT CGCGCTCCTG TTGACATCAC TGGTCAGTTT 
GAGAAGTGGG GAGAAGAAGA AGCAGAACTG AATAAGAACT TTTATGACAA GAAGACAGCA 
ATTCACAAAG CCCTCTGTGA CAATGTTGAC ACCCGCACCG TCATGGAAGA GATGCGGGCC 
TTGGTCAGTC AGTGCAACCT CTATATGGCA GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC 
CAGGCTCTGC TGGAGAACAT CGCCCTGTAC CTCACCCATA TGCTGAAGAT CTTTGGGGCC 
GTAGAAGAGG ACAGCTCCCT GGGATTCCCG GTCGGAGGGC CTGGAACCAG CCTCAGTCTC 
GAGGCCACAG TCATGCCCTA CCTTCAGGTG TTATCAGAAT TCCGAGAAGG AGTGCGGAAG 
ATTGCCCGAG AGCAAAAAGT CCCTGAGATT CTGCAGCTCA GCGATGCCCT GCGGGACAAC 
ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA GACCACGAAG GACTGCCCAC AGTGGTGAAA 
CTGGTAGACA GAAACACCTT ATTAAAAGAG AGAGAAGAAA AGAGACGGGT TGAAGAGGAG 
AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG AGGAAACAGG AACAAGAAGC AGCAAAGCTG 
GCCAAGATGA AGATTCCCCC CAGTGAGATG TTCTTGTCAG AAACCGACAA ATACTCCAAG 
TTTGATGAAA ATGTAAGCAT GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA 
AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13978
Clone ID Related Accession (Same CDS sequence) NM_001014437.3 , NM_001014437.2
Accession Version NM_001014437.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform c
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647906.1. On Jun 2, 2019 this sequence version replaced NM_001014437.2. Transcript Variant: This variant (3) includes an additional in-frame exon in the 5' coding region, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (c) has an additional segment in the N-terminal region and a distinct and longer C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647906.1, SRR1660809.176892.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC 
AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA 
TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001014437.1
CDS57..2552
Translation

Target ORF information:

RefSeq Version NM_001014437.3
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001014437.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC 
AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA 
TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13978
Clone ID Related Accession (Same CDS sequence) NM_001014437.3 , NM_001014437.2
Accession Version NM_001014437.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-16
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform c
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, BX647906.1 and AI432096.1. On Aug 17, 2010 this sequence version replaced NM_001014437.1. Transcript Variant: This variant (3) includes an additional in-frame exon in the 5' coding region, and uses an alternate splice site that causes a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (c) has an additional segment in the N-terminal region and a distinct and longer C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647906.1, SRR1803615.173119.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC 
AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA 
TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001014437.1
CDS85..2580
Translation

Target ORF information:

RefSeq Version NM_001014437.2
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001014437.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGGTCTGCCC ACACATGACA TGGAGGGCAA AGAGCTCAGC 
AAAGGGCAAG CCAAGAAGCT GAAGAAGCTC TTCGAGGCTC AGGAGAAGCT CTACAAGGAA 
TATCTGCAGA TGGCCCAGAA TGGAAGCTTC CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14615
Clone ID Related Accession (Same CDS sequence) NM_001194997.2 , NM_001194997.1
Accession Version NM_001194997.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-03
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform e
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AK298514.1, AC131971.9 and AI432096.1. On Jun 2, 2019 this sequence version replaced NM_001194997.1. Transcript Variant: This variant (5) includes an additional in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (e) that is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.81182.1, SRR1803613.150387.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG 
AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001181926.1
CDS57..2486
Translation

Target ORF information:

RefSeq Version NM_001194997.2
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001194997.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG 
AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14615
Clone ID Related Accession (Same CDS sequence) NM_001194997.2 , NM_001194997.1
Accession Version NM_001194997.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-16
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform e
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC376478.1, AK298514.1, AC131971.9 and AI432096.1. Transcript Variant: This variant (5) includes an additional in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (e) that is longer than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.47536.1, AK298514.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG 
AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001181926.1
CDS85..2514
Translation

Target ORF information:

RefSeq Version NM_001194997.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001194997.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGCACGG CAGTTGCGGC TGGCCTTCCT CATGCACTCG 
TGGAAGGACA CCCTGGACTA CTCCAGCAAC ACCATGGAGT CAGCGCTTCA ATATGAGAAG 
TTCTTGAATG AGTTTTTCTT AAATGTGAAA GATATCCTTC GCGCTCCTGT TGACATCACT 
GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA GCAGAACTGA ATAAGAACTT TTATGACAAG 
AAGACAGCAA TTCACAAAGC CCTCTGTGAC AATGTTGACA CCCGCACCGT CATGGAAGAG 
ATGCGGGCCT TGGTCAGTCA GTGCAACCTC TATATGGCAG CCCGGAAAGC CGTGAGGAAG 
AGGCCCAACC AGGCTCTGCT GGAGAACATC GCCCTGTACC TCACCCATAT GCTGAAGATC 
TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG GGATTCCCGG TCGGAGGGCC TGGAACCAGC 
CTCAGTCTCG AGGCCACAGT CATGCCCTAC CTTCAGGTGT TATCAGAATT CCGAGAAGGA 
GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC CCTGAGATTC TGCAGCTCAG CGATGCCCTG 
CGGGACAACA TCCTGCCCGA GCTTGGGGTG CGGTTTGAAG ACCACGAAGG ACTGCCCACA 
GTGGTGAAAC TGGTAGACAG AAACACCTTA TTAAAAGAGA GAGAAGAAAA GAGACGGGTT 
GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG GCGGCCCGGA GGAAACAGGA ACAAGAAGCA 
GCAAAGCTGG CCAAGATGAA GATTCCCCCC AGTGAGATGT TCTTGTCAGA AACCGACAAA 
TACTCCAAGT TTGATGAAAA TGTAAGCATG GTCTGCCCAC ACATGACATG GAGGGCAAAG 
AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu45024
Clone ID Related Accession (Same CDS sequence) XM_006718341.3
Accession Version XM_006718341.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718341.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG 
CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG 
TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT 
CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG 
AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC 
ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA 
GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC 
CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG 
GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG 
TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT 
CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA 
GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG 
AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG 
AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG 
TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGGTCTGCC CACACATGAC 
ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT 
CAGGAGAAGC TCTACAAGGA ATATCTGCAG ATGGCCCAGA ATGGAAGCTT CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006718404.1
CDS138..2354
Translation

Target ORF information:

RefSeq Version XM_006718341.3
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006718341.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG 
CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG 
TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT 
CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG 
AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC 
ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA 
GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC 
CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG 
GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG 
TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT 
CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA 
GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG 
AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG 
AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG 
TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGGTCTGCC CACACATGAC 
ATGGAGGGCA AAGAGCTCAG CAAAGGGCAA GCCAAGAAGC TGAAGAAGCT CTTCGAGGCT 
CAGGAGAAGC TCTACAAGGA ATATCTGCAG ATGGCCCAGA ATGGAAGCTT CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu103622
Clone ID Related Accession (Same CDS sequence) XM_024448701.1
Accession Version XM_024448701.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA 
CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG 
TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC 
CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG 
TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG 
AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT 
CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG 
GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG 
GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA 
GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG 
GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT 
GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC 
CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG 
GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG 
AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC 
AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT 
GATGAAAATG GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC 
AAGAAGCTGA AGAAGCTCTT CGAGGCTCAG GAGAAGCTCT ACAAGGAATA TCTGCAGATG 
GCCCAGAATG GAAGCTTCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024304469.1
CDS57..2660
Translation

Target ORF information:

RefSeq Version XM_024448701.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024448701.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA 
CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG 
TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC 
CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG 
TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG 
AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT 
CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG 
GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG 
GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA 
GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG 
GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT 
GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC 
CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG 
GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG 
AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC 
AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT 
GATGAAAATG GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC 
AAGAAGCTGA AGAAGCTCTT CGAGGCTCAG GAGAAGCTCT ACAAGGAATA TCTGCAGATG 
GCCCAGAATG GAAGCTTCCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu103623
Clone ID Related Accession (Same CDS sequence) XM_024448702.1
Accession Version XM_024448702.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA 
CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG 
TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC 
CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG 
TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG 
AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT 
CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG 
GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG 
GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA 
GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG 
GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT 
GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC 
CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG 
GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG 
AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC 
AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT 
GATGAAAATG TAAGCATGGT CTGCCCACAC ATGACATGGA GGGCAAAGAG CTCAGCAAAG 
GGCAAGCCAA GAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024304470.1
CDS57..2594
Translation

Target ORF information:

RefSeq Version XM_024448702.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024448702.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGGCAGATT CCTCCGGGCA GCAGGCTCCT GACTACAGGT CCATTCTGAG CATTAGTGAC 
GAGGCAGCCA GGGCACAAGC CCTGAACGAG CACCTCAGCA CGCGTAGCTA TGTCCAGGGG 
TACTCACTGT CCCAGGCAGA CGTGGACGCG TTCAGGCAGC TCTCGGCCCC GCCCGCTGAC 
CCCCAGCTCT TCCACGTGGC TCGGTGGTTC AGGCACATAG AAGCGCTCCT GGGTAGCCCC 
TGTGGCAAAG GCCAGCCCTG CAGGCTCCAA GCAAGCAAAG GCCGGCGTGT GCAGCCCCAG 
TGGTCCCCTC CTGCTGGGAC CCAGCCATGC AGACTCCACC TTTACAACAG CCTCACCAGG 
AACAAGGAAG TGTTCATACC TCAAGATGGG AAAAAGGTGA CGTGGTATTG CTGTGGGCCA 
ACCGTCTATG ACGCATCTCA CATGGGGCAC GCCAGGTCCT ACATCTCTTT TGATATCTTG 
AGAAGAGTGT TGAAGGATTA CTTCAAATTT GATGTCTTTT ATTGCATGAA CATTACGGAT 
ATTGATGACA AGATCATCAA GAGGGCCCGG CAGAACCACC TGTTCGAGCA GTATCGGGAG 
AAGAGGCCTG AAGCGGCACA GCTCTTGGAG GATGTTCAGG CCGCCCTGAA GCCATTTTCA 
GTAAAATTAA ATGAGACCAC GGATCCCGAT AAAAAGCAGA TGCTCGAACG GATTCAGCAC 
GCAGTGCAGC TTGCCACAGA GCCACTTGAG AAAGCTGTGC AGTCCAGACT CACGGGAGAG 
GAAGTCAACA GCTGTGTGGA GGTGTTGCTG GAAGAAGCCA AGGATTTGCT CTCTGACTGG 
CTGGATTCTA CACTTGGCTG TGATGTCACT GACAATTCCA TCTTCTCCAA GCTGCCCAAG 
TTCTGGGAGG GGGACTTCCA CAGAGACATG GAAGCTCTGA ATGTTCTCCC TCCAGATGTC 
TTAACCCGGG TTAGTGAGTA TGTGCCAGAA ATTGTGAACT TTGTCCAGAA GATTGTGGAC 
AACGGTTACG GCTATGTCTC CAATGGGTCT GTCTACTTTG ATACAGCGAA GTTTGCTTCT 
AGCGAGAAGC ACTCCTATGG GAAGCTGGTG CCTGAGGCCG TTGGAGATCA GAAAGCCCTT 
CAAGAAGGGG AAGGTGACCT GAGCATCTCT GCAGACCGCC TGAGTGAGAA GCGCTCTCCC 
AACGACTTTG CCTTATGGAA GGCCTCTAAG CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG 
GGAAAGGGTC GTCCGGGCTG GCATATCGAG TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG 
GCTTCGATGG ACATTCACGG AGGTGGGTTC GACCTCCGGT TCCCCCACCA TGACAATGAG 
CTGGCACAGT CGGAGGCCTA CTTTGAAAAC GACTGCTGGG TCAGGTACTT CCTGCACACA 
GGCCACCTGA CCATTGCAGG CTGCAAAATG TCAAAGTCAC TAAAAAACTT CATCACCATT 
AAAGATGCCT TGAAAAAGCA CTCAGGTATA CACTGGCTTC TCCCACAGAC CCCCCAACCA 
CCTTCTCATA CATGTGTAAT GGCTGTAGCA CTCGGGAGAA TAAGGGCGTT GGCTGTCTTG 
TGTCCCCCCA CAGCACGGCA GTTGCGGCTG GCCTTCCTCA TGCACTCGTG GAAGGACACC 
CTGGACTACT CCAGCAACAC CATGGAGTCA GCGCTTCAAT ATGAGAAGTT CTTGAATGAG 
TTTTTCTTAA ATGTGAAAGA TATCCTTCGC GCTCCTGTTG ACATCACTGG TCAGTTTGAG 
AAGTGGGGAG AAGAAGAAGC AGAACTGAAT AAGAACTTTT ATGACAAGAA GACAGCAATT 
CACAAAGCCC TCTGTGACAA TGTTGACACC CGCACCGTCA TGGAAGAGAT GCGGGCCTTG 
GTCAGTCAGT GCAACCTCTA TATGGCAGCC CGGAAAGCCG TGAGGAAGAG GCCCAACCAG 
GCTCTGCTGG AGAACATCGC CCTGTACCTC ACCCATATGC TGAAGATCTT TGGGGCCGTA 
GAAGAGGACA GCTCCCTGGG ATTCCCGGTC GGAGGGCCTG GAACCAGCCT CAGTCTCGAG 
GCCACAGTCA TGCCCTACCT TCAGGTGTTA TCAGAATTCC GAGAAGGAGT GCGGAAGATT 
GCCCGAGAGC AAAAAGTCCC TGAGATTCTG CAGCTCAGCG ATGCCCTGCG GGACAACATC 
CTGCCCGAGC TTGGGGTGCG GTTTGAAGAC CACGAAGGAC TGCCCACAGT GGTGAAACTG 
GTAGACAGAA ACACCTTATT AAAAGAGAGA GAAGAAAAGA GACGGGTTGA AGAGGAGAAG 
AGGAAGAAGA AAGAGGAGGC GGCCCGGAGG AAACAGGAAC AAGAAGCAGC AAAGCTGGCC 
AAGATGAAGA TTCCCCCCAG TGAGATGTTC TTGTCAGAAA CCGACAAATA CTCCAAGTTT 
GATGAAAATG TAAGCATGGT CTGCCCACAC ATGACATGGA GGGCAAAGAG CTCAGCAAAG 
GGCAAGCCAA GAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu103624
Clone ID Related Accession (Same CDS sequence) XM_024448703.1
Accession Version XM_024448703.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGGTAT ACACTGGCTT CTCCCACAGA CCCCCCAACC ACCTTCTCAT 
ACATGTGTAA TGGCTGTAGC ACTCGGGAGA ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC 
ACAGCACGGC AGTTGCGGCT GGCCTTCCTC ATGCACTCGT GGAAGGACAC CCTGGACTAC 
TCCAGCAACA CCATGGAGTC AGCGCTTCAA TATGAGAAGT TCTTGAATGA GTTTTTCTTA 
AATGTGAAAG ATATCCTTCG CGCTCCTGTT GACATCACTG GTCAGTTTGA GAAGTGGGGA 
GAAGAAGAAG CAGAACTGAA TAAGAACTTT TATGACAAGA AGACAGCAAT TCACAAAGCC 
CTCTGTGACA ATGTTGACAC CCGCACCGTC ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG 
TGCAACCTCT ATATGGCAGC CCGGAAAGCC GTGAGGAAGA GGCCCAACCA GGCTCTGCTG 
GAGAACATCG CCCTGTACCT CACCCATATG CTGAAGATCT TTGGGGCCGT AGAAGAGGAC 
AGCTCCCTGG GATTCCCGGT CGGAGGGCCT GGAACCAGCC TCAGTCTCGA GGCCACAGTC 
ATGCCCTACC TTCAGGTGTT ATCAGAATTC CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG 
CAAAAAGTCC CTGAGATTCT GCAGCTCAGC GATGCCCTGC GGGACAACAT CCTGCCCGAG 
CTTGGGGTGC GGTTTGAAGA CCACGAAGGA CTGCCCACAG TGGTGAAACT GGTAGACAGA 
AACACCTTAT TAAAAGAGAG AGAAGAAAAG AGACGGGTTG AAGAGGAGAA GAGGAAGAAG 
AAAGAGGAGG CGGCCCGGAG GAAACAGGAA CAAGAAGCAG CAAAGCTGGC CAAGATGAAG 
ATTCCCCCCA GTGAGATGTT CTTGTCAGAA ACCGACAAAT ACTCCAAGTT TGATGAAAAT 
GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG 
AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC TACAAGGAAT ATCTGCAGAT GGCCCAGAAT 
GGAAGCTTCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024304471.1
CDS57..2411
Translation

Target ORF information:

RefSeq Version XM_024448703.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024448703.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCAGATT CCTCCGGGCA GCAGGGCAAA GGCCGGCGTG TGCAGCCCCA GTGGTCCCCT 
CCTGCTGGGA CCCAGCCATG CAGACTCCAC CTTTACAACA GCCTCACCAG GAACAAGGAA 
GTGTTCATAC CTCAAGATGG GAAAAAGGTG ACGTGGTATT GCTGTGGGCC AACCGTCTAT 
GACGCATCTC ACATGGGGCA CGCCAGGTCC TACATCTCTT TTGATATCTT GAGAAGAGTG 
TTGAAGGATT ACTTCAAATT TGATGTCTTT TATTGCATGA ACATTACGGA TATTGATGAC 
AAGATCATCA AGAGGGCCCG GCAGAACCAC CTGTTCGAGC AGTATCGGGA GAAGAGGCCT 
GAAGCGGCAC AGCTCTTGGA GGATGTTCAG GCCGCCCTGA AGCCATTTTC AGTAAAATTA 
AATGAGACCA CGGATCCCGA TAAAAAGCAG ATGCTCGAAC GGATTCAGCA CGCAGTGCAG 
CTTGCCACAG AGCCACTTGA GAAAGCTGTG CAGTCCAGAC TCACGGGAGA GGAAGTCAAC 
AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC AAGGATTTGC TCTCTGACTG GCTGGATTCT 
ACACTTGGCT GTGATGTCAC TGACAATTCC ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG 
GGGGACTTCC ACAGAGACAT GGAAGCTCTG AATGTTCTCC CTCCAGATGT CTTAACCCGG 
GTTAGTGAGT ATGTGCCAGA AATTGTGAAC TTTGTCCAGA AGATTGTGGA CAACGGTTAC 
GGCTATGTCT CCAATGGGTC TGTCTACTTT GATACAGCGA AGTTTGCTTC TAGCGAGAAG 
CACTCCTATG GGAAGCTGGT GCCTGAGGCC GTTGGAGATC AGAAAGCCCT TCAAGAAGGG 
GAAGGTGACC TGAGCATCTC TGCAGACCGC CTGAGTGAGA AGCGCTCTCC CAACGACTTT 
GCCTTATGGA AGGCCTCTAA GCCCGGAGAA CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT 
CGTCCGGGCT GGCATATCGA GTGCTCGGCC ATGGCAGGCA CCCTCCTAGG GGCTTCGATG 
GACATTCACG GAGGTGGGTT CGACCTCCGG TTCCCCCACC ATGACAATGA GCTGGCACAG 
TCGGAGGCCT ACTTTGAAAA CGACTGCTGG GTCAGGTACT TCCTGCACAC AGGCCACCTG 
ACCATTGCAG GCTGCAAAAT GTCAAAGTCA CTAAAAAACT TCATCACCAT TAAAGATGCC 
TTGAAAAAGC ACTCAGGTAT ACACTGGCTT CTCCCACAGA CCCCCCAACC ACCTTCTCAT 
ACATGTGTAA TGGCTGTAGC ACTCGGGAGA ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC 
ACAGCACGGC AGTTGCGGCT GGCCTTCCTC ATGCACTCGT GGAAGGACAC CCTGGACTAC 
TCCAGCAACA CCATGGAGTC AGCGCTTCAA TATGAGAAGT TCTTGAATGA GTTTTTCTTA 
AATGTGAAAG ATATCCTTCG CGCTCCTGTT GACATCACTG GTCAGTTTGA GAAGTGGGGA 
GAAGAAGAAG CAGAACTGAA TAAGAACTTT TATGACAAGA AGACAGCAAT TCACAAAGCC 
CTCTGTGACA ATGTTGACAC CCGCACCGTC ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG 
TGCAACCTCT ATATGGCAGC CCGGAAAGCC GTGAGGAAGA GGCCCAACCA GGCTCTGCTG 
GAGAACATCG CCCTGTACCT CACCCATATG CTGAAGATCT TTGGGGCCGT AGAAGAGGAC 
AGCTCCCTGG GATTCCCGGT CGGAGGGCCT GGAACCAGCC TCAGTCTCGA GGCCACAGTC 
ATGCCCTACC TTCAGGTGTT ATCAGAATTC CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG 
CAAAAAGTCC CTGAGATTCT GCAGCTCAGC GATGCCCTGC GGGACAACAT CCTGCCCGAG 
CTTGGGGTGC GGTTTGAAGA CCACGAAGGA CTGCCCACAG TGGTGAAACT GGTAGACAGA 
AACACCTTAT TAAAAGAGAG AGAAGAAAAG AGACGGGTTG AAGAGGAGAA GAGGAAGAAG 
AAAGAGGAGG CGGCCCGGAG GAAACAGGAA CAAGAAGCAG CAAAGCTGGC CAAGATGAAG 
ATTCCCCCCA GTGAGATGTT CTTGTCAGAA ACCGACAAAT ACTCCAAGTT TGATGAAAAT 
GGTCTGCCCA CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG 
AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC TACAAGGAAT ATCTGCAGAT GGCCCAGAAT 
GGAAGCTTCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu103625
Clone ID Related Accession (Same CDS sequence) XM_024448704.1
Accession Version XM_024448704.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGGTAT ACACTGGCTT 
CTCCCACAGA CCCCCCAACC ACCTTCTCAT ACATGTGTAA TGGCTGTAGC ACTCGGGAGA 
ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC ACAGCACGGC AGTTGCGGCT GGCCTTCCTC 
ATGCACTCGT GGAAGGACAC CCTGGACTAC TCCAGCAACA CCATGGAGTC AGCGCTTCAA 
TATGAGAAGT TCTTGAATGA GTTTTTCTTA AATGTGAAAG ATATCCTTCG CGCTCCTGTT 
GACATCACTG GTCAGTTTGA GAAGTGGGGA GAAGAAGAAG CAGAACTGAA TAAGAACTTT 
TATGACAAGA AGACAGCAAT TCACAAAGCC CTCTGTGACA ATGTTGACAC CCGCACCGTC 
ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG TGCAACCTCT ATATGGCAGC CCGGAAAGCC 
GTGAGGAAGA GGCCCAACCA GGCTCTGCTG GAGAACATCG CCCTGTACCT CACCCATATG 
CTGAAGATCT TTGGGGCCGT AGAAGAGGAC AGCTCCCTGG GATTCCCGGT CGGAGGGCCT 
GGAACCAGCC TCAGTCTCGA GGCCACAGTC ATGCCCTACC TTCAGGTGTT ATCAGAATTC 
CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG CAAAAAGTCC CTGAGATTCT GCAGCTCAGC 
GATGCCCTGC GGGACAACAT CCTGCCCGAG CTTGGGGTGC GGTTTGAAGA CCACGAAGGA 
CTGCCCACAG TGGTGAAACT GGTAGACAGA AACACCTTAT TAAAAGAGAG AGAAGAAAAG 
AGACGGGTTG AAGAGGAGAA GAGGAAGAAG AAAGAGGAGG CGGCCCGGAG GAAACAGGAA 
CAAGAAGCAG CAAAGCTGGC CAAGATGAAG ATTCCCCCCA GTGAGATGTT CTTGTCAGAA 
ACCGACAAAT ACTCCAAGTT TGATGAAAAT GGTCTGCCCA CACATGACAT GGAGGGCAAA 
GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC 
TACAAGGAAT ATCTGCAGAT GGCCCAGAAT GGAAGCTTCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024304472.1
CDS378..2702
Translation

Target ORF information:

RefSeq Version XM_024448704.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024448704.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGGTAT ACACTGGCTT 
CTCCCACAGA CCCCCCAACC ACCTTCTCAT ACATGTGTAA TGGCTGTAGC ACTCGGGAGA 
ATAAGGGCGT TGGCTGTCTT GTGTCCCCCC ACAGCACGGC AGTTGCGGCT GGCCTTCCTC 
ATGCACTCGT GGAAGGACAC CCTGGACTAC TCCAGCAACA CCATGGAGTC AGCGCTTCAA 
TATGAGAAGT TCTTGAATGA GTTTTTCTTA AATGTGAAAG ATATCCTTCG CGCTCCTGTT 
GACATCACTG GTCAGTTTGA GAAGTGGGGA GAAGAAGAAG CAGAACTGAA TAAGAACTTT 
TATGACAAGA AGACAGCAAT TCACAAAGCC CTCTGTGACA ATGTTGACAC CCGCACCGTC 
ATGGAAGAGA TGCGGGCCTT GGTCAGTCAG TGCAACCTCT ATATGGCAGC CCGGAAAGCC 
GTGAGGAAGA GGCCCAACCA GGCTCTGCTG GAGAACATCG CCCTGTACCT CACCCATATG 
CTGAAGATCT TTGGGGCCGT AGAAGAGGAC AGCTCCCTGG GATTCCCGGT CGGAGGGCCT 
GGAACCAGCC TCAGTCTCGA GGCCACAGTC ATGCCCTACC TTCAGGTGTT ATCAGAATTC 
CGAGAAGGAG TGCGGAAGAT TGCCCGAGAG CAAAAAGTCC CTGAGATTCT GCAGCTCAGC 
GATGCCCTGC GGGACAACAT CCTGCCCGAG CTTGGGGTGC GGTTTGAAGA CCACGAAGGA 
CTGCCCACAG TGGTGAAACT GGTAGACAGA AACACCTTAT TAAAAGAGAG AGAAGAAAAG 
AGACGGGTTG AAGAGGAGAA GAGGAAGAAG AAAGAGGAGG CGGCCCGGAG GAAACAGGAA 
CAAGAAGCAG CAAAGCTGGC CAAGATGAAG ATTCCCCCCA GTGAGATGTT CTTGTCAGAA 
ACCGACAAAT ACTCCAAGTT TGATGAAAAT GGTCTGCCCA CACATGACAT GGAGGGCAAA 
GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG AAGAAGCTCT TCGAGGCTCA GGAGAAGCTC 
TACAAGGAAT ATCTGCAGAT GGCCCAGAAT GGAAGCTTCC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu103626
Clone ID Related Accession (Same CDS sequence) XM_024448705.1
Accession Version XM_024448705.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2079bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X7
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC 
CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC 
GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC 
AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT 
TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC 
TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT 
CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC 
CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA 
GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA 
GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT 
GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC 
TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA 
GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG 
TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA 
AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG GTATACACTG GCTTCTCCCA 
CAGACCCCCC AACCACCTTC TCATACATGT GTAATGGCTG TAGCACTCGG GAGAATAAGG 
GCGTTGGCTG TCTTGTGTCC CCCCACAGCA CGGCAGTTGC GGCTGGCCTT CCTCATGCAC 
TCGTGGAAGG ACACCCTGGA CTACTCCAGC AACACCATGG AGTCAGCGCT TCAATATGAG 
AAGTTCTTGA ATGAGTTTTT CTTAAATGTG AAAGATATCC TTCGCGCTCC TGTTGACATC 
ACTGGTCAGT TTGAGAAGTG GGGAGAAGAA GAAGCAGAAC TGAATAAGAA CTTTTATGAC 
AAGAAGACAG CAATTCACAA AGCCCTCTGT GACAATGTTG ACACCCGCAC CGTCATGGAA 
GAGATGCGGG CCTTGGTCAG TCAGTGCAAC CTCTATATGG CAGCCCGGAA AGCCGTGAGG 
AAGAGGCCCA ACCAGGCTCT GCTGGAGAAC ATCGCCCTGT ACCTCACCCA TATGCTGAAG 
ATCTTTGGGG CCGTAGAAGA GGACAGCTCC CTGGGATTCC CGGTCGGAGG GCCTGGAACC 
AGCCTCAGTC TCGAGGCCAC AGTCATGCCC TACCTTCAGG TGTTATCAGA ATTCCGAGAA 
GGAGTGCGGA AGATTGCCCG AGAGCAAAAA GTCCCTGAGA TTCTGCAGCT CAGCGATGCC 
CTGCGGGACA ACATCCTGCC CGAGCTTGGG GTGCGGTTTG AAGACCACGA AGGACTGCCC 
ACAGTGGTGA AACTGGTAGA CAGAAACACC TTATTAAAAG AGAGAGAAGA AAAGAGACGG 
GTTGAAGAGG AGAAGAGGAA GAAGAAAGAG GAGGCGGCCC GGAGGAAACA GGAACAAGAA 
GCAGCAAAGC TGGCCAAGAT GAAGATTCCC CCCAGTGAGA TGTTCTTGTC AGAAACCGAC 
AAATACTCCA AGTTTGATGA AAATGGTCTG CCCACACATG ACATGGAGGG CAAAGAGCTC 
AGCAAAGGGC AAGCCAAGAA GCTGAAGAAG CTCTTCGAGG CTCAGGAGAA GCTCTACAAG 
GAATATCTGC AGATGGCCCA GAATGGAAGC TTCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_024304473.1
CDS455..2533
Translation

Target ORF information:

RefSeq Version XM_024448705.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_024448705.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGAACATTA CGGATATTGA TGACAAGATC ATCAAGAGGG CCCGGCAGAA CCACCTGTTC 
GAGCAGTATC GGGAGAAGAG GCCTGAAGCG GCACAGCTCT TGGAGGATGT TCAGGCCGCC 
CTGAAGCCAT TTTCAGTAAA ATTAAATGAG ACCACGGATC CCGATAAAAA GCAGATGCTC 
GAACGGATTC AGCACGCAGT GCAGCTTGCC ACAGAGCCAC TTGAGAAAGC TGTGCAGTCC 
AGACTCACGG GAGAGGAAGT CAACAGCTGT GTGGAGGTGT TGCTGGAAGA AGCCAAGGAT 
TTGCTCTCTG ACTGGCTGGA TTCTACACTT GGCTGTGATG TCACTGACAA TTCCATCTTC 
TCCAAGCTGC CCAAGTTCTG GGAGGGGGAC TTCCACAGAG ACATGGAAGC TCTGAATGTT 
CTCCCTCCAG ATGTCTTAAC CCGGGTTAGT GAGTATGTGC CAGAAATTGT GAACTTTGTC 
CAGAAGATTG TGGACAACGG TTACGGCTAT GTCTCCAATG GGTCTGTCTA CTTTGATACA 
GCGAAGTTTG CTTCTAGCGA GAAGCACTCC TATGGGAAGC TGGTGCCTGA GGCCGTTGGA 
GATCAGAAAG CCCTTCAAGA AGGGGAAGGT GACCTGAGCA TCTCTGCAGA CCGCCTGAGT 
GAGAAGCGCT CTCCCAACGA CTTTGCCTTA TGGAAGGCCT CTAAGCCCGG AGAACCGTCC 
TGGCCGTGCC CTTGGGGAAA GGGTCGTCCG GGCTGGCATA TCGAGTGCTC GGCCATGGCA 
GGCACCCTCC TAGGGGCTTC GATGGACATT CACGGAGGTG GGTTCGACCT CCGGTTCCCC 
CACCATGACA ATGAGCTGGC ACAGTCGGAG GCCTACTTTG AAAACGACTG CTGGGTCAGG 
TACTTCCTGC ACACAGGCCA CCTGACCATT GCAGGCTGCA AAATGTCAAA GTCACTAAAA 
AACTTCATCA CCATTAAAGA TGCCTTGAAA AAGCACTCAG GTATACACTG GCTTCTCCCA 
CAGACCCCCC AACCACCTTC TCATACATGT GTAATGGCTG TAGCACTCGG GAGAATAAGG 
GCGTTGGCTG TCTTGTGTCC CCCCACAGCA CGGCAGTTGC GGCTGGCCTT CCTCATGCAC 
TCGTGGAAGG ACACCCTGGA CTACTCCAGC AACACCATGG AGTCAGCGCT TCAATATGAG 
AAGTTCTTGA ATGAGTTTTT CTTAAATGTG AAAGATATCC TTCGCGCTCC TGTTGACATC 
ACTGGTCAGT TTGAGAAGTG GGGAGAAGAA GAAGCAGAAC TGAATAAGAA CTTTTATGAC 
AAGAAGACAG CAATTCACAA AGCCCTCTGT GACAATGTTG ACACCCGCAC CGTCATGGAA 
GAGATGCGGG CCTTGGTCAG TCAGTGCAAC CTCTATATGG CAGCCCGGAA AGCCGTGAGG 
AAGAGGCCCA ACCAGGCTCT GCTGGAGAAC ATCGCCCTGT ACCTCACCCA TATGCTGAAG 
ATCTTTGGGG CCGTAGAAGA GGACAGCTCC CTGGGATTCC CGGTCGGAGG GCCTGGAACC 
AGCCTCAGTC TCGAGGCCAC AGTCATGCCC TACCTTCAGG TGTTATCAGA ATTCCGAGAA 
GGAGTGCGGA AGATTGCCCG AGAGCAAAAA GTCCCTGAGA TTCTGCAGCT CAGCGATGCC 
CTGCGGGACA ACATCCTGCC CGAGCTTGGG GTGCGGTTTG AAGACCACGA AGGACTGCCC 
ACAGTGGTGA AACTGGTAGA CAGAAACACC TTATTAAAAG AGAGAGAAGA AAAGAGACGG 
GTTGAAGAGG AGAAGAGGAA GAAGAAAGAG GAGGCGGCCC GGAGGAAACA GGAACAAGAA 
GCAGCAAAGC TGGCCAAGAT GAAGATTCCC CCCAGTGAGA TGTTCTTGTC AGAAACCGAC 
AAATACTCCA AGTTTGATGA AAATGGTCTG CCCACACATG ACATGGAGGG CAAAGAGCTC 
AGCAAAGGGC AAGCCAAGAA GCTGAAGAAG CTCTTCGAGG CTCAGGAGAA GCTCTACAAG 
GAATATCTGC AGATGGCCCA GAATGGAAGC TTCCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92567
Clone ID Related Accession (Same CDS sequence) XM_017018389.1 , XM_017018390.1
Accession Version XM_017018389.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG 
CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG 
TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT 
CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG 
AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC 
ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA 
GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC 
CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG 
GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG 
TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT 
CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA 
GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG 
AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG 
AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG 
TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA 
CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873878.1
CDS138..2288
Translation

Target ORF information:

RefSeq Version XM_017018389.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017018389.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG 
CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG 
TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT 
CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG 
AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC 
ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA 
GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC 
CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG 
GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG 
TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT 
CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA 
GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG 
AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG 
AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG 
TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA 
CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92567
Clone ID Related Accession (Same CDS sequence) XM_017018389.1 , XM_017018390.1
Accession Version XM_017018390.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X6
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG 
CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG 
TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT 
CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG 
AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC 
ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA 
GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC 
CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG 
GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG 
TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT 
CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA 
GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG 
AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG 
AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG 
TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA 
CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873879.1
CDS378..2528
Translation

Target ORF information:

RefSeq Version XM_017018390.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017018390.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGCAGACTC CACCTTTACA ACAGCCTCAC CAGGAACAAG TGTTTCTTGC ATTTCTTGTG 
ATCGTCATTC CTTCATTCCT AACAAAGGAA GTGTTCATAC CTCAAGATGG GAAAAAGGTG 
ACGTGGTATT GCTGTGGGCC AACCGTCTAT GACGCATCTC ACATGGGGCA CGCCAGGTCC 
TACATCTCTT TTGATATCTT GAGAAGAGTG TTGAAGGATT ACTTCAAATT TGATGTCTTT 
TATTGCATGA ACATTACGGA TATTGATGAC AAGATCATCA AGAGGGCCCG GCAGAACCAC 
CTGTTCGAGC AGTATCGGGA GAAGAGGCCT GAAGCGGCAC AGCTCTTGGA GGATGTTCAG 
GCCGCCCTGA AGCCATTTTC AGTAAAATTA AATGAGACCA CGGATCCCGA TAAAAAGCAG 
ATGCTCGAAC GGATTCAGCA CGCAGTGCAG CTTGCCACAG AGCCACTTGA GAAAGCTGTG 
CAGTCCAGAC TCACGGGAGA GGAAGTCAAC AGCTGTGTGG AGGTGTTGCT GGAAGAAGCC 
AAGGATTTGC TCTCTGACTG GCTGGATTCT ACACTTGGCT GTGATGTCAC TGACAATTCC 
ATCTTCTCCA AGCTGCCCAA GTTCTGGGAG GGGGACTTCC ACAGAGACAT GGAAGCTCTG 
AATGTTCTCC CTCCAGATGT CTTAACCCGG GTTAGTGAGT ATGTGCCAGA AATTGTGAAC 
TTTGTCCAGA AGATTGTGGA CAACGGTTAC GGCTATGTCT CCAATGGGTC TGTCTACTTT 
GATACAGCGA AGTTTGCTTC TAGCGAGAAG CACTCCTATG GGAAGCTGGT GCCTGAGGCC 
GTTGGAGATC AGAAAGCCCT TCAAGAAGGG GAAGGTGACC TGAGCATCTC TGCAGACCGC 
CTGAGTGAGA AGCGCTCTCC CAACGACTTT GCCTTATGGA AGGCCTCTAA GCCCGGAGAA 
CCGTCCTGGC CGTGCCCTTG GGGAAAGGGT CGTCCGGGCT GGCATATCGA GTGCTCGGCC 
ATGGCAGGCA CCCTCCTAGG GGCTTCGATG GACATTCACG GAGGTGGGTT CGACCTCCGG 
TTCCCCCACC ATGACAATGA GCTGGCACAG TCGGAGGCCT ACTTTGAAAA CGACTGCTGG 
GTCAGGTACT TCCTGCACAC AGGCCACCTG ACCATTGCAG GCTGCAAAAT GTCAAAGTCA 
CTAAAAAACT TCATCACCAT TAAAGATGCC TTGAAAAAGC ACTCAGCACG GCAGTTGCGG 
CTGGCCTTCC TCATGCACTC GTGGAAGGAC ACCCTGGACT ACTCCAGCAA CACCATGGAG 
TCAGCGCTTC AATATGAGAA GTTCTTGAAT GAGTTTTTCT TAAATGTGAA AGATATCCTT 
CGCGCTCCTG TTGACATCAC TGGTCAGTTT GAGAAGTGGG GAGAAGAAGA AGCAGAACTG 
AATAAGAACT TTTATGACAA GAAGACAGCA ATTCACAAAG CCCTCTGTGA CAATGTTGAC 
ACCCGCACCG TCATGGAAGA GATGCGGGCC TTGGTCAGTC AGTGCAACCT CTATATGGCA 
GCCCGGAAAG CCGTGAGGAA GAGGCCCAAC CAGGCTCTGC TGGAGAACAT CGCCCTGTAC 
CTCACCCATA TGCTGAAGAT CTTTGGGGCC GTAGAAGAGG ACAGCTCCCT GGGATTCCCG 
GTCGGAGGGC CTGGAACCAG CCTCAGTCTC GAGGCCACAG TCATGCCCTA CCTTCAGGTG 
TTATCAGAAT TCCGAGAAGG AGTGCGGAAG ATTGCCCGAG AGCAAAAAGT CCCTGAGATT 
CTGCAGCTCA GCGATGCCCT GCGGGACAAC ATCCTGCCCG AGCTTGGGGT GCGGTTTGAA 
GACCACGAAG GACTGCCCAC AGTGGTGAAA CTGGTAGACA GAAACACCTT ATTAAAAGAG 
AGAGAAGAAA AGAGACGGGT TGAAGAGGAG AAGAGGAAGA AGAAAGAGGA GGCGGCCCGG 
AGGAAACAGG AACAAGAAGC AGCAAAGCTG GCCAAGATGA AGATTCCCCC CAGTGAGATG 
TTCTTGTCAG AAACCGACAA ATACTCCAAG TTTGATGAAA ATGTAAGCAT GGTCTGCCCA 
CACATGACAT GGAGGGCAAA GAGCTCAGCA AAGGGCAAGC CAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92569
Clone ID Related Accession (Same CDS sequence) XM_017018392.2 , XM_017018392.1
Accession Version XM_017018392.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X9
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC 
AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG 
AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC 
GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT 
GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG 
CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG 
GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT 
AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG 
AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG 
GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT 
GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG 
GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA 
ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT 
GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG 
CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT 
GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG 
CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG 
TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC 
GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC 
GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG 
TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG 
CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC 
ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA 
GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA 
GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC 
AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC 
TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC 
GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG 
GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC 
CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC 
CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG 
CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA 
TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG 
GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC 
AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG 
GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873881.1
CDS42..2261
Translation

Target ORF information:

RefSeq Version XM_017018392.2
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase 1 (CARS1), transcript variant X18, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017018392.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC 
AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG 
AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC 
GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT 
GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG 
CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG 
GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT 
AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG 
AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG 
GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT 
GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG 
GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA 
ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT 
GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG 
CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT 
GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG 
CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG 
TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC 
GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC 
GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG 
TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG 
CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC 
ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA 
GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA 
GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC 
AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC 
TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC 
GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG 
GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC 
CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC 
CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG 
CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA 
TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG 
GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC 
AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG 
GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu92569
Clone ID Related Accession (Same CDS sequence) XM_017018392.2 , XM_017018392.1
Accession Version XM_017018392.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2220bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product cysteine--tRNA ligase, cytoplasmic isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC 
AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG 
AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC 
GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT 
GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG 
CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG 
GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT 
AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG 
AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG 
GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT 
GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG 
GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA 
ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT 
GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG 
CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT 
GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG 
CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG 
TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC 
GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC 
GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG 
TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG 
CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC 
ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA 
GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA 
GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC 
AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC 
TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC 
GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG 
GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC 
CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC 
CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG 
CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA 
TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG 
GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC 
AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG 
GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016873881.1
CDS231..2450
Translation

Target ORF information:

RefSeq Version XM_017018392.1
Organism Homo sapiens(human)
Definition Homo sapiens cysteinyl-tRNA synthetase (CARS), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017018392.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGAGCCTA CCTCAGGGCC TTTCCGCACA GACTTGGGAT ACCACTTGGT CGGCTGCTCA 
TCAGGCAAAG GCCGGCGTGT GCAGCCCCAG TGGTCCCCTC CTGCTGGGAC CCAGCCATGC 
AGACTCCACC TTTACAACAG CCTCACCAGG AACAAGGAAG TGTTCATACC TCAAGATGGG 
AAAAAGGTGA CGTGGTATTG CTGTGGGCCA ACCGTCTATG ACGCATCTCA CATGGGGCAC 
GCCAGGTCCT ACATCTCTTT TGATATCTTG AGAAGAGTGT TGAAGGATTA CTTCAAATTT 
GATGTCTTTT ATTGCATGAA CATTACGGAT ATTGATGACA AGATCATCAA GAGGGCCCGG 
CAGAACCACC TGTTCGAGCA GTATCGGGAG AAGAGGCCTG AAGCGGCACA GCTCTTGGAG 
GATGTTCAGG CCGCCCTGAA GCCATTTTCA GTAAAATTAA ATGAGACCAC GGATCCCGAT 
AAAAAGCAGA TGCTCGAACG GATTCAGCAC GCAGTGCAGC TTGCCACAGA GCCACTTGAG 
AAAGCTGTGC AGTCCAGACT CACGGGAGAG GAAGTCAACA GCTGTGTGGA GGTGTTGCTG 
GAAGAAGCCA AGGATTTGCT CTCTGACTGG CTGGATTCTA CACTTGGCTG TGATGTCACT 
GACAATTCCA TCTTCTCCAA GCTGCCCAAG TTCTGGGAGG GGGACTTCCA CAGAGACATG 
GAAGCTCTGA ATGTTCTCCC TCCAGATGTC TTAACCCGGG TTAGTGAGTA TGTGCCAGAA 
ATTGTGAACT TTGTCCAGAA GATTGTGGAC AACGGTTACG GCTATGTCTC CAATGGGTCT 
GTCTACTTTG ATACAGCGAA GTTTGCTTCT AGCGAGAAGC ACTCCTATGG GAAGCTGGTG 
CCTGAGGCCG TTGGAGATCA GAAAGCCCTT CAAGAAGGGG AAGGTGACCT GAGCATCTCT 
GCAGACCGCC TGAGTGAGAA GCGCTCTCCC AACGACTTTG CCTTATGGAA GGCCTCTAAG 
CCCGGAGAAC CGTCCTGGCC GTGCCCTTGG GGAAAGGGTC GTCCGGGCTG GCATATCGAG 
TGCTCGGCCA TGGCAGGCAC CCTCCTAGGG GCTTCGATGG ACATTCACGG AGGTGGGTTC 
GACCTCCGGT TCCCCCACCA TGACAATGAG CTGGCACAGT CGGAGGCCTA CTTTGAAAAC 
GACTGCTGGG TCAGGTACTT CCTGCACACA GGCCACCTGA CCATTGCAGG CTGCAAAATG 
TCAAAGTCAC TAAAAAACTT CATCACCATT AAAGATGCCT TGAAAAAGCA CTCAGCACGG 
CAGTTGCGGC TGGCCTTCCT CATGCACTCG TGGAAGGACA CCCTGGACTA CTCCAGCAAC 
ACCATGGAGT CAGCGCTTCA ATATGAGAAG TTCTTGAATG AGTTTTTCTT AAATGTGAAA 
GATATCCTTC GCGCTCCTGT TGACATCACT GGTCAGTTTG AGAAGTGGGG AGAAGAAGAA 
GCAGAACTGA ATAAGAACTT TTATGACAAG AAGACAGCAA TTCACAAAGC CCTCTGTGAC 
AATGTTGACA CCCGCACCGT CATGGAAGAG ATGCGGGCCT TGGTCAGTCA GTGCAACCTC 
TATATGGCAG CCCGGAAAGC CGTGAGGAAG AGGCCCAACC AGGCTCTGCT GGAGAACATC 
GCCCTGTACC TCACCCATAT GCTGAAGATC TTTGGGGCCG TAGAAGAGGA CAGCTCCCTG 
GGATTCCCGG TCGGAGGGCC TGGAACCAGC CTCAGTCTCG AGGCCACAGT CATGCCCTAC 
CTTCAGGTGT TATCAGAATT CCGAGAAGGA GTGCGGAAGA TTGCCCGAGA GCAAAAAGTC 
CCTGAGATTC TGCAGCTCAG CGATGCCCTG CGGGACAACA TCCTGCCCGA GCTTGGGGTG 
CGGTTTGAAG ACCACGAAGG ACTGCCCACA GTGGTGAAAC TGGTAGACAG AAACACCTTA 
TTAAAAGAGA GAGAAGAAAA GAGACGGGTT GAAGAGGAGA AGAGGAAGAA GAAAGAGGAG 
GCGGCCCGGA GGAAACAGGA ACAAGAAGCA GCAAAGCTGG CCAAGATGAA GATTCCCCCC 
AGTGAGATGT TCTTGTCAGA AACCGACAAA TACTCCAAGT TTGATGAAAA TGTAAGCATG 
GTCTGCCCAC ACATGACATG GAGGGCAAAG AGCTCAGCAA AGGGCAAGCC AAGAAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Polymorphisms in CARS are associated with gastric cancer risk: a two-stage case-control study in the Chinese population.
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association20(6)940-947(2017 Nov)
Tian T,Xiao L,Du J,Zhu X,Gu Y,Qin N,Yan C,Liu L,Ma H,Jiang Y,Chen J,Yu H,Dai J


book

Loss of cysteinyl-tRNA synthetase (CARS) induces the transsulfuration pathway and inhibits ferroptosis induced by cystine deprivation.
Cell death and differentiation23(2)270-8(2016 Feb)
Hayano M,Yang WS,Corn CK,Pagano NC,Stockwell BR


book

Evaluation of associations between single nucleotide polymorphisms in the FRMD3 and CARS genes and diabetic nephropathy in a Kuwaiti population.
Genetics and molecular research : GMR15(1)(2016 Jan)
Al-waheeb S,Alwohhaib M,Abdelghani A,Al-Sharrah S,Al-Shafey E,Al-Sahow A,Alyatama N,Dashti AA,Hussain N


book

Meta-analysis of genome-wide association studies identifies six new Loci for serum calcium concentrations.
PLoS genetics9(9)e1003796(2013)
O'Seaghdha CM,Wu H,Yang Q,Kapur K,Guessous I,Zuber AM,K?ttgen A,Stoudmann C,Teumer A,Kutalik Z,Mangino M,Dehghan A,Zhang W,Eiriksdottir G,Li G,Tanaka T,Portas L,Lopez LM,Hayward C,Lohman K,Matsuda K,Padmanabhan S,Firsov D,Sorice R,Ulivi S,Brockhaus AC,Kleber ME,Mahajan A,Ernst FD,Gudnason V,Launer LJ,Mace A,Boerwinckle E,Arking DE,Tanikawa C,Nakamura Y,Brown MJ,Gaspoz JM,Theler JM,Siscovick DS,Psaty BM,Bergmann S,Vollenweider P,Vitart V,Wright AF,Zemunik T,Boban M,Kolcic I,Navarro P,Brown EM,Estrada K,Ding J,Harris TB,Bandinelli S,Hernandez D,Singleton AB,Girotto G,Ruggiero D,d'Adamo AP,Robino A,Meitinger T,Meisinger C,Davies G,Starr JM,Chambers JC,Boehm BO,Winkelmann BR,Huang J,Murgia F,Wild SH,Campbell H,Morris AP,Franco OH,Hofman A,Uitterlinden AG,Rivadeneira F,V?lker U,Hannemann A,Biffar R,Hoffmann W,Shin SY,Lescuyer P,Henry H,Schurmann C,,,Munroe PB,Gasparini P,Pirastu N,Ciullo M,Gieger C,M?rz W,Lind L,Spector TD,Smith AV,Rudan I,Wilson JF,Polasek O,Deary IJ,Pirastu M,Ferrucci L,Liu Y,Kestenbaum B,Kooner JS,Witteman JC,Nauck M,Kao WH,Wallaschofski H,Bonny O,Fox CS,Bochud M