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HIST1H2BE cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HIST1H2BE
Entrez Gene ID 8344
Full Name histone cluster 1 H2B family member e
Synonyms H2B.h,H2B/h,H2BFH,dJ221C16.8
General protein information
Preferred Names

histone cluster 1 H2B family member e

Names

histone H2B type 1-C/E/F/G/I
H2B histone family, member H
histone 1, H2be
histone H2B.1 A
histone H2B.h
histone cluster 1, H2be

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6p22.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015].
Disorder MIM:

602805


mRNA and Protein(s)

mRNA Protein Name
NM_003523.2 NP_003514.2 histone H2B type 1-C/E/F/G/I



Bos taurus (cattle) HIST1H2BL XP_002686101.1
Pan troglodytes (chimpanzee) LOC471883 XP_527261.1
Canis lupus familiaris (dog) LOC100856116 XP_003640206.1
Mus musculus (house mouse) Hist1h2bl NP_835506.1
Homo sapiens (human) HIST1H2BE NP_003514.2


Related articles in PubMed

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y
Molecular cell23(4)607-18(2006 Aug)

Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II.
Pavri R, Zhu B, Li G, Trojer P, Mandal S, Shilatifard A, Reinberg D
Cell125(4)703-17(2006 May)

Antimicrobial peptides in the first line defence of human colon mucosa.
Tollin M, Bergman P, Svenberg T, Jörnvall H, Gudmundsson GH, Agerberth B
Peptides24(4)523-30(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Histone H2B antimicrobial peptides participate in the colonic defense against bacteria and fungi.
Title: Antimicrobial peptides in the first line defence of human colon mucosa.

The following HIST1H2BE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H2BE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu30268 NM_003523.2
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Homo sapiens histone cluster 1 H2B family member e (HIST1H2BE), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30268
Clone ID Related Accession (Same CDS sequence) NM_003523.2
Accession Version NM_003523.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 381bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product histone H2B type 1-C/E/F/G/I
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z80780.1. On Jun 12, 2002 this sequence version replaced NM_003523.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BP362977.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 12860195 replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGCCTGAGC CAGCGAAATC CGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCCGTGACC 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA
TACGTGTACA AGGTGCTGAA ACAGGTCCAC CCCGACACCG GCATCTCCTC TAAAGCCATG
GGGATCATGA ATTCCTTTGT CAACGACATC TTCGAGCGCA TCGCCGGCGA GGCTTCCCGC
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC
CTGCTGCTTC CCGGGGAGCT GGCCAAGCAC GCTGTGTCAG AGGGCACCAA GGCCGTTACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003514.2
CDS1..381
Misc Feature(1)4..6
Misc Feature(2)7..306
Misc Feature(3)16..18
Misc Feature(4)16..18
Misc Feature(5)34..36
Misc Feature(6)34..36
Misc Feature(7)37..39
Misc Feature(8)37..39
Misc Feature(9)43..45
Misc Feature(10)46..48
Misc Feature(11)46..48
Misc Feature(12)49..51
Misc Feature(13)49..51
Misc Feature(14)61..63
Misc Feature(15)61..63
Misc Feature(16)70..72
Misc Feature(17)70..72
Misc Feature(18)82..372
Misc Feature(19)103..105
Misc Feature(20)109..111
Misc Feature(21)139..141
Misc Feature(22)172..174
Misc Feature(23)238..240
Misc Feature(24)256..258
Misc Feature(25)256..258
Misc Feature(26)259..261
Misc Feature(27)277..279
Misc Feature(28)325..327
Misc Feature(29)346..348
Misc Feature(30)349..351
Exon (1)1..435
Translation

Target ORF information:

RefSeq Version NM_003523.2
Organism Homo sapiens(Human)
Definition Homo sapiens histone cluster 1 H2B family member e (HIST1H2BE), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003523.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCCTGAGC CAGCGAAATC CGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCCGTGACC 
AAGGCGCAGA AGAAGGACGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTACTCCGTA
TACGTGTACA AGGTGCTGAA ACAGGTCCAC CCCGACACCG GCATCTCCTC TAAAGCCATG
GGGATCATGA ATTCCTTTGT CAACGACATC TTCGAGCGCA TCGCCGGCGA GGCTTCCCGC
CTGGCGCATT ACAACAAGCG CTCGACCATC ACCTCCAGGG AGATCCAGAC GGCCGTGCGC
CTGCTGCTTC CCGGGGAGCT GGCCAAGCAC GCTGTGTCAG AGGGCACCAA GGCCGTTACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballescà JL,Oliva R


book

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W


book

Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
Molecular cell23(4)607-18(2006 Aug)
Kim SC,Sprung R,Chen Y,Xu Y,Ball H,Pei J,Cheng T,Kho Y,Xiao H,Xiao L,Grishin NV,White M,Yang XJ,Zhao Y


book

Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II.
Cell125(4)703-17(2006 May)
Pavri R,Zhu B,Li G,Trojer P,Mandal S,Shilatifard A,Reinberg D


book

Antimicrobial peptides in the first line defence of human colon mucosa.
Peptides24(4)523-30(2003 Apr)
Tollin M,Bergman P,Svenberg T,Jörnvall H,Gudmundsson GH,Agerberth B


 
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