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HIST1H2BI cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HIST1H2BI
Entrez Gene ID 8346
Full Name histone cluster 1 H2B family member i
Synonyms H2B/k,H2BFK
General protein information
Preferred Names

histone cluster 1 H2B family member i

Names

histone H2B type 1-C/E/F/G/I
H2B histone family, member K
histone 1, H2bi
histone H2B.1 A
histone H2B.k
histone cluster 1, H2bi

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

6

6p22.2

Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. The protein has antibacterial and antifungal antimicrobial activity. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015].
Disorder MIM:

602807


mRNA and Protein(s)

mRNA Protein Name
NM_003525.2 NP_003516.1 histone H2B type 1-C/E/F/G/I



Rattus norvegicus (Norway rat) Hist1h2bh NP_001100822.1
Homo sapiens (human) HIST1H2BI NP_003516.1
Pan troglodytes (chimpanzee) LOC471888 XP_527266.3
Bos taurus (cattle) LOC616776 XP_002697507.1
Mus musculus (house mouse) Hist1h2bc NP_075911.2
Canis lupus familiaris (dog) LOC100856186 XP_003640209.1


Related articles in PubMed

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Flachsbart F, Franke A, Kleindorp R, Caliebe A, Blanché H, Schreiber S, Nebel A
Mutation research694(1-2)13-9(2010 Dec)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

A modified "cross-talk" between histone H2B Lys-120 ubiquitination and H3 Lys-79 methylation.
Darwanto A, Curtis MP, Schrag M, Kirsch W, Liu P, Xu G, Neidigh JW, Zhang K
The Journal of biological chemistry285(28)21868-76(2010 Jul)

Regulation of HIV-1 gene expression by histone acetylation and factor recruitment at the LTR promoter.
Lusic M, Marcello A, Cereseto A, Giacca M
The EMBO journal22(24)6550-61(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.

a catalytic and inhibitory loop mechanism may better describe the cross-talk relationship between H2B ubiquitination and H3 Lys-79 methylation
Title: A modified "cross-talk" between histone H2B Lys-120 ubiquitination and H3 Lys-79 methylation.

Histone H2B antimicrobial peptides participate in the colonic defense against bacteria and fungi.
Title: Antimicrobial peptides in the first line defence of human colon mucosa.

The following HIST1H2BI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIST1H2BI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu30099 NM_003525.2
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Homo sapiens histone cluster 1 H2B family member i (HIST1H2BI), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30099
Clone ID Related Accession (Same CDS sequence) NM_003525.2
Accession Version NM_003525.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 381bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product histone H2B type 1-C/E/F/G/I
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z80782.1. On May 24, 2002 this sequence version replaced NM_003525.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: DA509995.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 12860195 replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGCCTGAAC CAGCTAAGTC AGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCGGTGACC 
AAGGCACAGA AGAAGGATGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTATTCCGTG
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCGTC CAAGGCTATG
GGGATTATGA ACTCCTTCGT CAACGACATT TTCGAGCGCA TTGCAGGCGA GGCTTCCCGC
CTGGCGCATT ATAACAAGCG CTCGACCATC ACTTCCAGGG AGATCCAAAC GGCTGTGCGC
CTGCTGCTAC CCGGGGAGCT GGCCAAACAC GCGGTGTCGG AGGGCACCAA GGCGGTCACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003516.1
CDS1..381
Misc Feature(1)4..6
Misc Feature(2)7..306
Misc Feature(3)16..18
Misc Feature(4)16..18
Misc Feature(5)34..36
Misc Feature(6)34..36
Misc Feature(7)37..39
Misc Feature(8)37..39
Misc Feature(9)43..45
Misc Feature(10)46..48
Misc Feature(11)46..48
Misc Feature(12)49..51
Misc Feature(13)49..51
Misc Feature(14)61..63
Misc Feature(15)61..63
Misc Feature(16)70..72
Misc Feature(17)70..72
Misc Feature(18)82..372
Misc Feature(19)103..105
Misc Feature(20)109..111
Misc Feature(21)139..141
Misc Feature(22)172..174
Misc Feature(23)238..240
Misc Feature(24)256..258
Misc Feature(25)256..258
Misc Feature(26)259..261
Misc Feature(27)277..279
Misc Feature(28)325..327
Misc Feature(29)346..348
Misc Feature(30)349..351
Exon (1)1..437
Translation

Target ORF information:

RefSeq Version NM_003525.2
Organism Homo sapiens(Human)
Definition Homo sapiens histone cluster 1 H2B family member i (HIST1H2BI), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003525.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGCCTGAAC CAGCTAAGTC AGCTCCCGCC CCGAAGAAGG GCTCCAAGAA GGCGGTGACC 
AAGGCACAGA AGAAGGATGG CAAGAAGCGC AAGCGCAGCC GCAAGGAGAG CTATTCCGTG
TACGTGTACA AGGTGCTGAA GCAGGTCCAC CCCGACACCG GCATCTCGTC CAAGGCTATG
GGGATTATGA ACTCCTTCGT CAACGACATT TTCGAGCGCA TTGCAGGCGA GGCTTCCCGC
CTGGCGCATT ATAACAAGCG CTCGACCATC ACTTCCAGGG AGATCCAAAC GGCTGTGCGC
CTGCTGCTAC CCGGGGAGCT GGCCAAACAC GCGGTGTCGG AGGGCACCAA GGCGGTCACC
AAGTACACCA GCTCCAAGTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballescà JL,Oliva R


book

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Mutation research694(1-2)13-9(2010 Dec)
Flachsbart F,Franke A,Kleindorp R,Caliebe A,Blanché H,Schreiber S,Nebel A


book

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W


book

A modified "cross-talk" between histone H2B Lys-120 ubiquitination and H3 Lys-79 methylation.
The Journal of biological chemistry285(28)21868-76(2010 Jul)
Darwanto A,Curtis MP,Schrag M,Kirsch W,Liu P,Xu G,Neidigh JW,Zhang K


book

Regulation of HIV-1 gene expression by histone acetylation and factor recruitment at the LTR promoter.
The EMBO journal22(24)6550-61(2003 Dec)
Lusic M,Marcello A,Cereseto A,Giacca M


 
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