HASPIN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HASPIN
Entrez Gene ID	83903
Full Name	histone H3 associated protein kinase
Synonyms	GSG2
General protein information	Preferred Names histone H3 associated protein kinase Names serine/threonine-protein kinase haspin H-haspin germ cell associated 2, haspin germ cell-specific gene 2 protein haploid germ cell-specific nuclear protein kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.2
Disorder MIM:	609240

mRNA and Protein(s)

mRNA	Protein	Name
NM_031965.2	NP_114171.2	serine/threonine-protein kinase haspin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GSG2	XP_854539.1
Pan troglodytes (chimpanzee)	GSG2	XP_001159222.1
	GSG2	XP_001090778.1
Mus musculus (house mouse)	Gsg2	NP_034483.1
Rattus norvegicus (Norway rat)	Gsg2	XP_003750897.1
Bos taurus (cattle)	GSG2	NP_001070012.1
Homo sapiens (human)	GSG2	NP_114171.2

Gene Ontology
Bibliography

Related articles in PubMed

High-resolution Imaging in Male Germ Cell-Associated Kinase (MAK)-related Retinal Degeneration.
Lew YJ, Rinella N, Qin J, Chiang J, Moore AT, Porco TC, Roorda A, Duncan JL
American journal of ophthalmology18532-42(2018 Jan)

A kinase-dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion.
Liang C, Chen Q, Yi Q, Zhang M, Yan H, Zhang B, Zhou L, Zhang Z, Qi F, Ye S, Wang F
EMBO reports19(1)43-56(2018 Jan)

Pds5 Regulates Sister-Chromatid Cohesion and Chromosome Bi-orientation through a Conserved Protein Interaction Module.
Goto Y, Yamagishi Y, Shintomi-Kawamura M, Abe M, Tanno Y, Watanabe Y
Current biology : CB27(7)1005-1012(2017 Apr)

The N-Terminal Non-Kinase-Domain-Mediated Binding of Haspin to Pds5B Protects Centromeric Cohesion in Mitosis.
Zhou L, Liang C, Chen Q, Zhang Z, Zhang B, Yan H, Qi F, Zhang M, Yi Q, Guan Y, Xiang X, Zhang X, Ye S, Wang F
Current biology : CB27(7)992-1004(2017 Apr)

Structure, Roles and Inhibitors of a Mitotic Protein Kinase Haspin.
Kestav K, Uri A, Lavogina D
Current medicinal chemistry24(21)2276-2293(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Autosomal recessive RP associated with mutations in the male germ cell-associated kinase (MAK) gene is associated with preservation of foveal vision even in advanced stages of retinal degeneration despite similar rates of peripheral visual field loss to other forms of autosomal recessive RP
Title: High-resolution Imaging in Male Germ Cell-Associated Kinase (MAK)-related Retinal Degeneration.

This is the first structural model of a bisubstrate inhibitor targeting haspin. The presented structural data represent a model for the future development of more specific haspin inhibitors
Title: Co-crystal structures of the protein kinase haspin with bisubstrate inhibitors.

Haspin kinase plays a key role in maintaining the slowly exchanging centromere Borealin pool.
Title: Borealin dimerization mediates optimal CPC checkpoint function by enhancing localization to centromeres and kinetochores.

Haspin activity affects the phosphorylation state of proteins involved in gene expression regulation and splicing.
Title: Modulation of the chromatin phosphoproteome by the Haspin protein kinase.

Haspin inhibitors reveal centromeric roles of Aurora B in chromosome movement and spindle checkpoint signaling
Title: Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation.

The following HASPIN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HASPIN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu31161	NM_031965.2 Latest version!	Homo sapiens histone H3 associated protein kinase (HASPIN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HASPIN ( NM_031965.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_114171.2 Homo sapiens histone H3 associated protein kinase (HASPIN), mRNA.

Price & Availability↑

CloneID	OHu31161
Clone ID Related Accession (Same CDS sequence)	NM_031965.2
Accession Version	NM_031965.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase haspin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC036181.1, BC016626.1, BC047457.1 and AF289865.1. On Dec 23, 2004 this sequence version replaced NM_031965.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC036181.1, AB039834.3 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325418.5/ ENSP00000325290.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGCGGCTT CGCTCCCGGG ACCTGGGAGC CGGCTTTTCC GCACATATGG GGCTGCGGAC 
GGCAGGAGAC AGCGGCGGCC GGGCCGGGAA GCCGCGCAGT GGTTCCCGCC GCAGGACCGG 
AGGCGTTTCT TCAACAGCAG CGGCAGCAGC GACGCCAGCA TCGGCGACCC CTCGCAGTCC 
GACGATCCTG ACGATCCCGA CGACCCCGAC TTCCCCGGCA GCCCGGTGAG GCGGCGGCGG 
AGGCGTCCCG GCGGCCGAGT GCCCAAGGAC CGGCCCAGCC TGACCGTGAC CCCAAAGCGC 
TGGAAGCTGC GAGCTCGCCC AAGCCTAACC GTGACCCCAA GACGCCTGGG GCTGCGAGCT 
CGGCCCCCGC AGAAGTGCAG CACACCCTGC GGCCCGCTCC GACTTCCGCC CTTCCCCAGC 
CGCGACTCCG GCCGCCTCAG CCCGGACCTC AGCGTGTGCG GCCAGCCCAG GGACGGCGAC 
GAGCTGGGCA TCAGTGCCTC CCTGTTCAGC TCTCTGGCCT CGCCCTGCCC CGGGTCCCCA 
ACGCCAAGGG ACAGTGTCAT CTCGATCGGC ACCTCCGCCT GTCTGGTTGC AGCCTCAGCC 
GTCCCGAGCG GCCTCCACCT CCCAGAAGTC TCCCTGGACC GAGCATCTCT CCCCTGCTCC 
CAGGAGGAAG CGACAGGAGG AGCCAAGGAC ACCAGGATGG TCCACCAAAC CCGCGCCAGC 
CTCAGGTCAG TTCTCTTTGG CCTTATGAAC TCAGGAACCC CTGAGGATTC TGAGTTTCGG 
GCAGATGGGA AGAATATGAG AGAGTCCTGC TGTAAAAGGA AACTGGTGGT GGGAAATGGA 
CCAGAGGGTC CAGGTCTGTC AAGCACAGGC AAGAGGAGGG CCACAGGCCA GGACTCTTGT 
CAAGAGAGAG GGCTTCAAGA GGCCGTCCGG AGAGAGCATC AGGAGGCCAG TGTTCCCAAG 
GGCCGCATTG TGCCAAGGGG AATAGACAGG CTGGAGAGAA CTAGATCAAG CCGGAAGAGC 
AAACATCAGG AGGCAACGGA AACCTCTCTC CTCCATTCCC ACCGCTTTAA AAAGGGCCAA 
AAGCTGGGAA AAGATTCGTT CCCCACCCAG GACCTGACTC CTTTACAGAA TGTCTGCTTT 
TGGACCAAAA CCAGGGCTTC CTTCAGTTTC CACAAGAAGA AAATTGTGAC TGATGTGTCA 
GAGGTCTGCA GCATCTATAC CACTGCCACT TCTCTCTCTG GATCCCTCCT ATCAGAATGT 
TCAAACCGGC CTGTCATGAA CAGAACAAGT GGTGCTCCGT CCTCTTGGCA CTCCTCCTCT 
ATGTATTTGC TAAGCCCCTT AAACACTCTA AGTATTTCAA ACAAAAAGGC ATCTGATGCT 
GAAAAGGTTT ATGGGGAATG CAGTCAGAAG GGTCCTGTCC CCTTTAGCCA TTGCCTTCCC 
ACAGAAAAAC TGCAACGCTG TGAGAAGATT GGGGAAGGGG TGTTTGGCGA AGTGTTTCAA 
ACAATTGCTG ATCACACACC CGTAGCCATA AAAATCATTG CTATTGAAGG ACCAGATTTA 
GTCAATGGAT CCCATCAGAA AACCTTTGAG GAAATCCTGC CAGAGATCAT CATCTCCAAA 
GAGTTGAGCC TCTTATCCGG TGAAGTGTGC AACCGCACAG AAGGCTTTAT CGGGCTGAAC 
TCAGTGCACT GTGTCCAGGG ATCTTACCCT CCCTTGCTCC TCAAAGCCTG GGATCACTAT 
AATTCAACCA AAGGCTCTGC AAATGACCGG CCTGATTTTT TTAAAGACGA CCAGCTCTTC 
ATTGTGCTGG AATTTGAGTT TGGAGGGATT GACTTAGAGC AAATGCGAAC CAAGTTGTCT 
TCCTTGGCTA CTGCAAAGAG CATTCTACAC CAGCTCACAG CCTCCCTCGC AGTGGCAGAG 
GCATCACTGC GCTTTGAGCA CCGAGACTTA CACTGGGGGA ACGTGCTCTT AAAGAAAACC 
AGCCTCAAAA AACTCCACTA CACCCTCAAT GGGAAGAGCA GCACTATCCC CAGCTGTGGG 
TTGCAAGTGA GCATCATTGA CTACACCCTG TCGCGCTTGG AACGGGATGG GATTGTGGTT 
TTCTGTGACG TTTCCATGGA TGAGGACCTG TTTACCGGTG ACGGTGACTA CCAGTTTGAC 
ATCTACAGGC TCATGAAGAA GGAGAATAAC AACCGCTGGG GTGAATATCA CCCTTATAGT 
AATGTGCTCT GGTTACATTA CCTGACAGAC AAGATGCTGA AACAAATGAC CTTCAAGACT 
AAATGTAACA CTCCTGCCAT GAAGCAAATT AAGAGAAAAA TCCAGGAGTT CCACAGGACA 
ATGCTGAACT TCAGCTCTGC CACTGACTTG CTCTGCCAGC ACAGTCTGTT TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114171.2
CDS	34..2430
Translation	MAASLPGPGSRLFRTYGAADGRRQRRPGREAAQWFPPQDRRRFFNSSGSSDASIGDPSQSDDPDDPDDPDFPGSPVRRRRRRPGGRVPKDRPSLTVTPKRWKLRARPSLTVTPRRLGLRARPPQKCSTPCGPLRLPPFPSRDSGRLSPDLSVCGQPRDGDELGISASLFSSLASPCPGSPTPRDSVISIGTSACLVAASAVPSGLHLPEVSLDRASLPCSQEEATGGAKDTRMVHQTRASLRSVLFGLMNSGTPEDSEFRADGKNMRESCCKRKLVVGNGPEGPGLSSTGKRRATGQDSCQERGLQEAVRREHQEASVPKGRIVPRGIDRLERTRSSRKSKHQEATETSLLHSHRFKKGQKLGKDSFPTQDLTPLQNVCFWTKTRASFSFHKKKIVTDVSEVCSIYTTATSLSGSLLSECSNRPVMNRTSGAPSSWHSSSMYLLSPLNTLSISNKKASDAEKVYGECSQKGPVPFSHCLPTEKLQRCEKIGEGVFGEVFQTIADHTPVAIKIIAIEGPDLVNGSHQKTFEEILPEIIISKELSLLSGEVCNRTEGFIGLNSVHCVQGSYPPLLLKAWDHYNSTKGSANDRPDFFKDDQLFIVLEFEFGGIDLEQMRTKLSSLATAKSILHQLTASLAVAEASLRFEHRDLHWGNVLLKKTSLKKLHYTLNGKSSTIPSCGLQVSIIDYTLSRLERDGIVVFCDVSMDEDLFTGDGDYQFDIYRLMKKENNNRWGEYHPYSNVLWLHYLTDKMLKQMTFKTKCNTPAMKQIKRKIQEFHRTMLNFSSATDLLCQHSLFK

Target ORF information:

RefSeq Version	NM_031965.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histone H3 associated protein kinase (HASPIN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031965.2

ORF Insert Sequence:

ATGGCGGCTT CGCTCCCGGG ACCTGGGAGC CGGCTTTTCC GCACATATGG GGCTGCGGAC 
GGCAGGAGAC AGCGGCGGCC GGGCCGGGAA GCCGCGCAGT GGTTCCCGCC GCAGGACCGG 
AGGCGTTTCT TCAACAGCAG CGGCAGCAGC GACGCCAGCA TCGGCGACCC CTCGCAGTCC 
GACGATCCTG ACGATCCCGA CGACCCCGAC TTCCCCGGCA GCCCGGTGAG GCGGCGGCGG 
AGGCGTCCCG GCGGCCGAGT GCCCAAGGAC CGGCCCAGCC TGACCGTGAC CCCAAAGCGC 
TGGAAGCTGC GAGCTCGCCC AAGCCTAACC GTGACCCCAA GACGCCTGGG GCTGCGAGCT 
CGGCCCCCGC AGAAGTGCAG CACACCCTGC GGCCCGCTCC GACTTCCGCC CTTCCCCAGC 
CGCGACTCCG GCCGCCTCAG CCCGGACCTC AGCGTGTGCG GCCAGCCCAG GGACGGCGAC 
GAGCTGGGCA TCAGTGCCTC CCTGTTCAGC TCTCTGGCCT CGCCCTGCCC CGGGTCCCCA 
ACGCCAAGGG ACAGTGTCAT CTCGATCGGC ACCTCCGCCT GTCTGGTTGC AGCCTCAGCC 
GTCCCGAGCG GCCTCCACCT CCCAGAAGTC TCCCTGGACC GAGCATCTCT CCCCTGCTCC 
CAGGAGGAAG CGACAGGAGG AGCCAAGGAC ACCAGGATGG TCCACCAAAC CCGCGCCAGC 
CTCAGGTCAG TTCTCTTTGG CCTTATGAAC TCAGGAACCC CTGAGGATTC TGAGTTTCGG 
GCAGATGGGA AGAATATGAG AGAGTCCTGC TGTAAAAGGA AACTGGTGGT GGGAAATGGA 
CCAGAGGGTC CAGGTCTGTC AAGCACAGGC AAGAGGAGGG CCACAGGCCA GGACTCTTGT 
CAAGAGAGAG GGCTTCAAGA GGCCGTCCGG AGAGAGCATC AGGAGGCCAG TGTTCCCAAG 
GGCCGCATTG TGCCAAGGGG AATAGACAGG CTGGAGAGAA CTAGATCAAG CCGGAAGAGC 
AAACATCAGG AGGCAACGGA AACCTCTCTC CTCCATTCCC ACCGCTTTAA AAAGGGCCAA 
AAGCTGGGAA AAGATTCGTT CCCCACCCAG GACCTGACTC CTTTACAGAA TGTCTGCTTT 
TGGACCAAAA CCAGGGCTTC CTTCAGTTTC CACAAGAAGA AAATTGTGAC TGATGTGTCA 
GAGGTCTGCA GCATCTATAC CACTGCCACT TCTCTCTCTG GATCCCTCCT ATCAGAATGT 
TCAAACCGGC CTGTCATGAA CAGAACAAGT GGTGCTCCGT CCTCTTGGCA CTCCTCCTCT 
ATGTATTTGC TAAGCCCCTT AAACACTCTA AGTATTTCAA ACAAAAAGGC ATCTGATGCT 
GAAAAGGTTT ATGGGGAATG CAGTCAGAAG GGTCCTGTCC CCTTTAGCCA TTGCCTTCCC 
ACAGAAAAAC TGCAACGCTG TGAGAAGATT GGGGAAGGGG TGTTTGGCGA AGTGTTTCAA 
ACAATTGCTG ATCACACACC CGTAGCCATA AAAATCATTG CTATTGAAGG ACCAGATTTA 
GTCAATGGAT CCCATCAGAA AACCTTTGAG GAAATCCTGC CAGAGATCAT CATCTCCAAA 
GAGTTGAGCC TCTTATCCGG TGAAGTGTGC AACCGCACAG AAGGCTTTAT CGGGCTGAAC 
TCAGTGCACT GTGTCCAGGG ATCTTACCCT CCCTTGCTCC TCAAAGCCTG GGATCACTAT 
AATTCAACCA AAGGCTCTGC AAATGACCGG CCTGATTTTT TTAAAGACGA CCAGCTCTTC 
ATTGTGCTGG AATTTGAGTT TGGAGGGATT GACTTAGAGC AAATGCGAAC CAAGTTGTCT 
TCCTTGGCTA CTGCAAAGAG CATTCTACAC CAGCTCACAG CCTCCCTCGC AGTGGCAGAG 
GCATCACTGC GCTTTGAGCA CCGAGACTTA CACTGGGGGA ACGTGCTCTT AAAGAAAACC 
AGCCTCAAAA AACTCCACTA CACCCTCAAT GGGAAGAGCA GCACTATCCC CAGCTGTGGG 
TTGCAAGTGA GCATCATTGA CTACACCCTG TCGCGCTTGG AACGGGATGG GATTGTGGTT 
TTCTGTGACG TTTCCATGGA TGAGGACCTG TTTACCGGTG ACGGTGACTA CCAGTTTGAC 
ATCTACAGGC TCATGAAGAA GGAGAATAAC AACCGCTGGG GTGAATATCA CCCTTATAGT 
AATGTGCTCT GGTTACATTA CCTGACAGAC AAGATGCTGA AACAAATGAC CTTCAAGACT 
AAATGTAACA CTCCTGCCAT GAAGCAAATT AAGAGAAAAA TCCAGGAGTT CCACAGGACA 
ATGCTGAACT TCAGCTCTGC CACTGACTTG CTCTGCCAGC ACAGTCTGTT TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-resolution Imaging in Male Germ Cell-Associated Kinase (MAK)-related Retinal Degeneration.
American journal of ophthalmology18532-42(2018 Jan)
Lew YJ,Rinella N,Qin J,Chiang J,Moore AT,Porco TC,Roorda A,Duncan JL

A kinase-dependent role for Haspin in antagonizing Wapl and protecting mitotic centromere cohesion.
EMBO reports19(1)43-56(2018 Jan)
Liang C,Chen Q,Yi Q,Zhang M,Yan H,Zhang B,Zhou L,Zhang Z,Qi F,Ye S,Wang F

Pds5 Regulates Sister-Chromatid Cohesion and Chromosome Bi-orientation through a Conserved Protein Interaction Module.
Current biology : CB27(7)1005-1012(2017 Apr)
Goto Y,Yamagishi Y,Shintomi-Kawamura M,Abe M,Tanno Y,Watanabe Y

The N-Terminal Non-Kinase-Domain-Mediated Binding of Haspin to Pds5B Protects Centromeric Cohesion in Mitosis.
Current biology : CB27(7)992-1004(2017 Apr)
Zhou L,Liang C,Chen Q,Zhang Z,Zhang B,Yan H,Qi F,Zhang M,Yi Q,Guan Y,Xiang X,Zhang X,Ye S,Wang F

Structure, Roles and Inhibitors of a Mitotic Protein Kinase Haspin.
Current medicinal chemistry24(21)2276-2293(2017)
Kestav K,Uri A,Lavogina D

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HASPIN cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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