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MAGT1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGT1
Entrez Gene ID 84061
Full Name magnesium transporter 1
Synonyms IAP,MRX95,OST3B,PRO0756,XMEN,bA217H1.1
General protein information
Preferred Names

magnesium transporter 1

Names

magnesium transporter protein 1
implantation-associated protein
oligosaccharyltransferase 3 homolog B

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq21.1

Summary This gene encodes a magnesium cation transporter protein that localizes to the cell membrane. This protein also associates with N-oligosaccharyl transferase and therefore may have a role in N-glycosylation. Mutations in this gene cause mental retardation X-linked type 95 (MRX95). This gene may have multiple in-frame translation initiation sites, one of which would encode a shorter protein with an N-terminus containing a signal peptide at amino acids 1-29. [provided by RefSeq, Jan 2010].
Disorder MIM:

300715

Disorder Html: Mental retardation, X-linked 95, 300716 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_032121.5 NP_115497.4 magnesium transporter protein 1



Bos taurus (cattle) MAGT1 NP_001231247.1
Rattus norvegicus (Norway rat) Magt1 NP_446398.1
Gallus gallus (chicken) MAGT1 NP_001006435.1
Macaca mulatta (rhesus monkey) LOC710098 XP_002806337.1
Canis lupus familiaris (dog) MAGT1 XP_549095.3
Mus musculus (house mouse) Magt1 NP_001177338.1
Homo sapiens (human) MAGT1 NP_115497.4
Danio rerio (zebrafish) magt1 NP_955994.1
Xenopus tropicalis (tropical clawed frog) magt1 XP_002931857.1


Related articles in PubMed

Up-regulation of AKAP13 and MAGT1 on cytoplasmic membrane in progressive hepatocellular carcinoma: a novel target for prognosis.
Molee P, Adisakwattana P, Reamtong O, Petmitr S, Sricharunrat T, Suwandittakul N, Chaisri U
International journal of clinical and experimental pathology8(9)9796-811(2015)

Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
Cherepanova NA, Shrimal S, Gilmore R
The Journal of cell biology206(4)525-39(2014 Aug)

Variant ATRX syndrome with dysfunction of ATRX and MAGT1 genes.
Qiao Y, Mondal K, Trapani V, Wen J, Carpenter G, Wildin R, Price EM, Gibbons RJ, Eichmeyer J, Jiang R, DuPont B, Martell S, Lewis SM, Robinson WP, O'Driscoll M, Wolf FI, Zwick ME, Rajcan-Separovic E
Human mutation35(1)58-62(2014 Jan)

Loss of MAGT1 abrogates the Mg2+ flux required for T cell signaling and leads to a novel human primary immunodeficiency.
Li FY, Lenardo MJ, Chaigne-Delalande B
Magnesium research24(3)S109-14(2011 Sep)

The Mg2+ transporter MagT1 partially rescues cell growth and Mg2+ uptake in cells lacking the channel-kinase TRPM7.
Deason-Towne F, Perraud AL, Schmitz C
FEBS letters585(14)2275-8(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

evaluation of MAGT1 and AKAP13 expression in clinical hepatocellular carcinoma tissues by immunohistochemistry suggested that both proteins were strongly expressed in tumor tissues with significantly higher average immunoreactive scores of Remmele and Stegner (IRS) than in non-tumor tissues
Title: Up-regulation of AKAP13 and MAGT1 on cytoplasmic membrane in progressive hepatocellular carcinoma: a novel target for prognosis.

A 0.8 kb intronic duplication in MAGT1 and a single base pair deletion in the last exon of ATRX were identified in a family with five males demonstrating intellectual disability (ID) and unusual skin findings (e.g., generalized pruritus).
Title: Variant ATRX syndrome with dysfunction of ATRX and MAGT1 genes.

A deficiency in magnesium transporter 1 (MAGT1), an Mg(2+)-specific transporter, leads to the absence of a T cell antigen receptor-stimulated Mg(2+)flux and an attenuation of T cell activation.
Title: Loss of MAGT1 abrogates the Mg2+ flux required for T cell signaling and leads to a novel human primary immunodeficiency.

Data show that gene expression of the Mg(2+) selective transporter MagT1 is upregulated in TRPM7(-/-) cells.
Title: The Mg2+ transporter MagT1 partially rescues cell growth and Mg2+ uptake in cells lacking the channel-kinase TRPM7.

MagT1 is universally expressed in all tissues and its expression level is up-regulated in low extracellular Mg(2+). Knockdown of either MagT1 or TUSC3 protein significantly lowers the total and free intracellular Mg(2+) concentrations in cell lines.
Title: Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development.

The following MAGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18683 NM_032121.5
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Homo sapiens magnesium transporter 1 (MAGT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18683
Clone ID Related Accession (Same CDS sequence) NM_032121.5
Accession Version NM_032121.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1104bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-05
Organism Homo sapiens(Human)
Product magnesium transporter protein 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC329979.1, BC060842.1 and BC041014.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2008 this sequence version replaced NM_032121.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060842.1, SRR1660805.237223.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGGAAAG GCAAGGGGCC GATTTGCCTG TTCTCACGCC CCACCCTCAG ACCTAGCCGG 
AGCAAAGTTT CACTTATAGA AGGGAGAGGA GCGAACATGG CAGCGCGTTG GCGGTTTTGG
TGTGTCTCTG TGACCATGGT GGTGGCGCTG CTCATCGTTT GCGACGTTCC CTCAGCCTCT
GCCCAAAGAA AGAAGGAGAT GGTGTTATCT GAAAAGGTTA GTCAGCTGAT GGAATGGACT
AACAAAAGAC CTGTAATAAG AATGAATGGA GACAAGTTCC GTCGCCTTGT GAAAGCCCCA
CCGAGAAATT ACTCCGTTAT CGTCATGTTC ACTGCTCTCC AACTGCATAG ACAGTGTGTC
GTTTGCAAGC AAGCTGATGA AGAATTCCAG ATCCTGGCAA ACTCCTGGCG ATACTCCAGT
GCATTCACCA ACAGGATATT TTTTGCCATG GTGGATTTTG ATGAAGGCTC TGATGTATTT
CAGATGCTAA ACATGAATTC AGCTCCAACT TTCATCAACT TTCCTGCAAA AGGGAAACCC
AAACGGGGTG ATACATATGA GTTACAGGTG CGGGGTTTTT CAGCTGAGCA GATTGCCCGG
TGGATCGCCG ACAGAACTGA TGTCAATATT AGAGTGATTA GACCCCCAAA TTATGCTGGT
CCCCTTATGT TGGGATTGCT TTTGGCTGTT ATTGGTGGAC TTGTGTATCT TCGAAGAAGT
AATATGGAAT TTCTCTTTAA TAAAACTGGA TGGGCTTTTG CAGCTTTGTG TTTTGTGCTT
GCTATGACAT CTGGTCAAAT GTGGAACCAT ATAAGAGGAC CACCATATGC CCATAAGAAT
CCCCACACGG GACATGTGAA TTATATCCAT GGAAGCAGTC AAGCCCAGTT TGTAGCTGAA
ACACACATTG TTCTTCTGTT TAATGGTGGA GTTACCTTAG GAATGGTGCT TTTATGTGAA
GCTGCTACCT CTGACATGGA TATTGGAAAG CGAAAGATAA TGTGTGTGGC TGGTATTGGA
CTTGTTGTAT TATTCTTCAG TTGGATGCTC TCTATTTTTA GATCTAAATA TCATGGCTAC
CCATACAGCT TTCTGATGAG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_115497.4
CDS63..1166
Misc Feature(1)51..53
Misc Feature(2)339..671
Misc Feature(3)order(417..419,426..428)
Misc Feature(4)657..1106
Exon (1)1..260
Exon (2)261..430
Exon (3)431..548
Exon (4)549..689
Exon (5)690..830
Exon (6)831..920
Exon (7)921..984
Exon (8)985..1059
Exon (9)1060..1150
Exon (10)1151..4022
Translation

Target ORF information:

RefSeq Version NM_032121.5
Organism Homo sapiens(Human)
Definition Homo sapiens magnesium transporter 1 (MAGT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_032121.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAGGAAAG GCAAGGGGCC GATTTGCCTG TTCTCACGCC CCACCCTCAG ACCTAGCCGG 
AGCAAAGTTT CACTTATAGA AGGGAGAGGA GCGAACATGG CAGCGCGTTG GCGGTTTTGG
TGTGTCTCTG TGACCATGGT GGTGGCGCTG CTCATCGTTT GCGACGTTCC CTCAGCCTCT
GCCCAAAGAA AGAAGGAGAT GGTGTTATCT GAAAAGGTTA GTCAGCTGAT GGAATGGACT
AACAAAAGAC CTGTAATAAG AATGAATGGA GACAAGTTCC GTCGCCTTGT GAAAGCCCCA
CCGAGAAATT ACTCCGTTAT CGTCATGTTC ACTGCTCTCC AACTGCATAG ACAGTGTGTC
GTTTGCAAGC AAGCTGATGA AGAATTCCAG ATCCTGGCAA ACTCCTGGCG ATACTCCAGT
GCATTCACCA ACAGGATATT TTTTGCCATG GTGGATTTTG ATGAAGGCTC TGATGTATTT
CAGATGCTAA ACATGAATTC AGCTCCAACT TTCATCAACT TTCCTGCAAA AGGGAAACCC
AAACGGGGTG ATACATATGA GTTACAGGTG CGGGGTTTTT CAGCTGAGCA GATTGCCCGG
TGGATCGCCG ACAGAACTGA TGTCAATATT AGAGTGATTA GACCCCCAAA TTATGCTGGT
CCCCTTATGT TGGGATTGCT TTTGGCTGTT ATTGGTGGAC TTGTGTATCT TCGAAGAAGT
AATATGGAAT TTCTCTTTAA TAAAACTGGA TGGGCTTTTG CAGCTTTGTG TTTTGTGCTT
GCTATGACAT CTGGTCAAAT GTGGAACCAT ATAAGAGGAC CACCATATGC CCATAAGAAT
CCCCACACGG GACATGTGAA TTATATCCAT GGAAGCAGTC AAGCCCAGTT TGTAGCTGAA
ACACACATTG TTCTTCTGTT TAATGGTGGA GTTACCTTAG GAATGGTGCT TTTATGTGAA
GCTGCTACCT CTGACATGGA TATTGGAAAG CGAAAGATAA TGTGTGTGGC TGGTATTGGA
CTTGTTGTAT TATTCTTCAG TTGGATGCTC TCTATTTTTA GATCTAAATA TCATGGCTAC
CCATACAGCT TTCTGATGAG TTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Up-regulation of AKAP13 and MAGT1 on cytoplasmic membrane in progressive hepatocellular carcinoma: a novel target for prognosis.
International journal of clinical and experimental pathology8(9)9796-811(2015)
Molee P,Adisakwattana P,Reamtong O,Petmitr S,Sricharunrat T,Suwandittakul N,Chaisri U


book

Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.
The Journal of cell biology206(4)525-39(2014 Aug)
Cherepanova NA,Shrimal S,Gilmore R


book

Variant ATRX syndrome with dysfunction of ATRX and MAGT1 genes.
Human mutation35(1)58-62(2014 Jan)
Qiao Y,Mondal K,Trapani V,Wen J,Carpenter G,Wildin R,Price EM,Gibbons RJ,Eichmeyer J,Jiang R,DuPont B,Martell S,Lewis SM,Robinson WP,O'Driscoll M,Wolf FI,Zwick ME,Rajcan-Separovic E


book

Loss of MAGT1 abrogates the Mg2+ flux required for T cell signaling and leads to a novel human primary immunodeficiency.
Magnesium research24(3)S109-14(2011 Sep)
Li FY,Lenardo MJ,Chaigne-Delalande B


book

The Mg2+ transporter MagT1 partially rescues cell growth and Mg2+ uptake in cells lacking the channel-kinase TRPM7.
FEBS letters585(14)2275-8(2011 Jul)
Deason-Towne F,Perraud AL,Schmitz C


 
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