×

AP3B1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol AP3B1
Entrez Gene ID 8546
Full Name adaptor related protein complex 3 beta 1 subunit
Synonyms ADTB3,ADTB3A,HPS,HPS2,PE
General protein information
Preferred Names

adaptor related protein complex 3 beta 1 subunit

Names

AP-3 complex subunit beta-1
AP-3 complex beta-3A subunit
adaptor protein complex AP-3 subunit beta-1
adaptor-related protein complex 3 subunit beta-1
beta-3A-adaptin
clathrin assembly protein complex 3 beta-1 large chain

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q14.1

Summary This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:

603401

Disorder Html: Hermansky-Pudlak syndrome 2, 608233 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005248618.3 XP_005248675.1 AP-3 complex subunit beta-1 isoform X1
NM_001271769.1 NP_001258698.1 AP-3 complex subunit beta-1 isoform 2
XM_017010001.1 XP_016865490.1 AP-3 complex subunit beta-1 isoform X2
XM_005248619.4 XP_005248676.1 AP-3 complex subunit beta-1 isoform X3
NM_003664.4 NP_003655.3 AP-3 complex subunit beta-1 isoform 1



Bos taurus (cattle) AP3B1 NP_001070002.1
Schizosaccharomyces pombe (fission yeast) apl6 NP_593796.1
Homo sapiens (human) AP3B1 NP_003655.3
Pan troglodytes (chimpanzee) AP3B1 XP_517689.2
Canis lupus familiaris (dog) AP3B1 NP_001002974.1
Mus musculus (house mouse) Ap3b1 NP_033810.2
Gallus gallus (chicken) AP3B1 XP_003643003.1
Drosophila melanogaster (fruit fly) rb NP_525071.2
Macaca mulatta (rhesus monkey) AP3B1 XP_002804484.1


Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Synergistic defects of UNC13D and AP3B1 leading to adult hemophagocytic lymphohistiocytosis.
Gao L, Zhu L, Huang L, Zhou J
International journal of hematology102(4)488-92(2015 Oct)

Matrix proteins of Nipah and Hendra viruses interact with beta subunits of AP-3 complexes.
Sun W, McCrory TS, Khaw WY, Petzing S, Myers T, Schmitt AP
Journal of virology88(22)13099-110(2014 Nov)

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
Bultema JJ, Ambrosio AL, Burek CL, Di Pietro SM
The Journal of biological chemistry287(23)19550-63(2012 Jun)

AP-3 and Rabip4' coordinately regulate spatial distribution of lysosomes.
Ivan V, Martinez-Sanchez E, Sima LE, Oorschot V, Klumperman J, Petrescu SM, van der Sluijs P
PloS one7(10)e48142(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Synergistic defects of UNC13D and AP3B1 leading to adult hemophagocytic lymphohistiocytosis.
Title: Synergistic defects of UNC13D and AP3B1 leading to adult hemophagocytic lymphohistiocytosis.

Using a co-affinity purification strategy, authors have identified the beta subunit of the AP-3 adapter protein complex, AP3B1, as a binding partner for the M proteins of the zoonotic paramyxoviruses Nipah virus and Hendra virus.
Title: Matrix proteins of Nipah and Hendra viruses interact with beta subunits of AP-3 complexes.

Data indicate that RUN and FYVE domain protein Rabip4'(RUFY1) interacts specifically and directly with adaptor protein complex AP-3.
Title: AP-3 and Rabip4' coordinately regulate spatial distribution of lysosomes.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following AP3B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP3B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu39764 XM_005248619.4
Latest version!
Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu96173 XM_017010001.1
Latest version!
Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $559.30
$799.00
OHu21104 NM_003664.4
Latest version!
Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu21364 NM_001271769.1
Latest version!
Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu39763 XM_005248618.3
Latest version!
Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu39764
Clone ID Related Accession (Same CDS sequence) XM_005248619.4
Accession Version XM_005248619.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product AP-3 complex subunit beta-1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248619.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGTCCAGCA ATAGTTTTCC TTACAATGAG CAGTCCGGAG GAGGGGAGGC GACGGAGCTG 
GGTCAGGAGG CGACCTCAAC CATTTCCCCC TCGGGGGCCT TCGGCCTCTT TAGCAGCGAT
TTGAAGAAGA ATGAAGATCT AAAGCAAATG TTAGAGAGCA ACAAAGATTC TGCTAAACTG
GATGCTATGA AGCGGATTGT TGGGATGATT GCAAAAGGGA AAAATGCATC TGAACTGTTT
CCTGCTGTTG TGAAGAATGT GGCCAGTAAA AATATTGAGA TCAAGAAGTT GGTATATGTT
TACCTGGTTC GATATGCTGA AGAACAGCAG GATCTTGCAC TCCTGTCCAT AAGCACTTTT
CAGCGAGCTC TGAAGGACCC AAACCAACTA ATTCGTGCAA GCGCTTTGAG AGTTCTGTCA
AGTATTAGAG TGCCAATTAT TGTACCTATC ATGATGCTTG CTATTAAGGA AGCTTCTGCT
GACTTATCAC CATATGTTAG GAAGAATGCA GCCCATGCAA TACAAAAATT ATACAGCCTT
GATCCAGAGC AGAAGGAAAT GTTAATTGAA GTAATTGAAA AACTTCTGAA AGATAAAAGC
ACATTGGTAG CTGGCAGTGT TGTGATGGCT TTTGAAGAAG TATGCCCGGA CAGAATAGAT
CTGATTCATA AAAATTACCG CAAGCTATGT AACTTACTAG TGGATGTTGA AGAGTGGGGG
CAGGTTGTCA TAATCCACAT GCTAACTCGA TATGCTCGGA CACAGTTTGT CAGCCCTTGG
AAAGAGGGTG ATGAATTAGA AGACAATGGA AAGAATTTCT ACGAATCTGA TGATGATCAG
AAGGAAAAGA CTGACAAAAA GAAGAAGCCG TATACTATGG ATCCAGATCA TAGACTCTTA
ATTAGAAATA CAAAGCCTTT GCTTCAGAGC AGGAATGCTG CGGTGGTTAT GGCAGTTGCT
CAGCTGTATT GGCACATATC ACCAAAATCT GAAGCTGGCA TAATTTCTAA ATCACTAGTG
CGTTTACTTC GTAGCAATAG GGAGGTGCAG TATATTGTCC TACAAAATAT AGCAACTATG
TCAATTCAAA GAAAGGGGAT GTTTGAACCT TATCTGAAGA GTTTCTATGT TAGGTCAACT
GATCCAACTA TGATCAAGAC ACTGAAGCTT GAAATTTTGA CAAACTTGGC AAATGAAGCC
AACATATCAA CTCTTCTTCG AGAATTTCAG ACCTATGTGA AAAGCCAGGA TAAACAATTT
GCAGCAGCCA CTATTCAGAC TATAGGCAGA TGTGCAACCA ACATCTTGGA AGTCACTGAC
ACGTGCCTCA ATGGCTTGGT CTGTCTGCTG TCCAACAGGG ATGAAATAGT TGTTGCTGAA
AGTGTGGTTG TTATAAAGAA ATTACTGCAA ATGCAACCTG CACAACATGG TGAAATTATT
AAACATATGG CCAAACTCCT GGACAGTATC ACTGTTCCTG TTGCTAGAGC AAGTATTCTT
TGGCTAATTG GAGAAAACTG TGAACGAGTT CCTAAAATTG CCCCTGATGT TTTGAGGAAG
ATGGCTAAAA GCTTCACTAG TGAAGATGAT CTGGTAAAAC TGCAGATATT AAATCTGGGA
GCAAAATTGT ATTTAACCAA CTCCAAACAG ACAAAATTGC TTACCCAGTA CATATTAAAT
CTCGGCAAGT ATGATCAAAA CTACGACATC AGAGACCGTA CAAGATTTAT TAGGCAGCTT
ATTGTTCCGA ATGTAAAGAG TGGAGCTTTA AGTAAATATG CCAAAAAAAT ATTCCTAGCA
CAAAAGCCTG CACCACTGCT TGAGTCTCCT TTTAAAGATA GAGATCATTT CCAGCTTGGC
ACCTTATCTC ATACTCTCAA CATTAAAGCT ACTGGGTACC TGGAATTATC TAATTGGCCA
GAGGTGGCGC CCGACCCATC AGTTCGAAAT GTAGAAGTAA TAGAGTTGGC AAAAGAATGG
ACCCCAGCAG GAAAAGCAAA GCAAGAGAAT TCTGCTAAGA AGTTTTATTC TGAATCTGAG
GAAGAGGAGG ACTCTTCTGA TAGTAGCAGT GACAGTGAGA GTGAATCTGG AAGTGAAAGT
GGAGAACAAG GCGAAAGTGG GGAGGAAGGA GACAGCAATG AGGACAGCAG TGAGGACTCC
TCCAGTGAGC AGGACAGTGA GAGTGGACGG GAGTCAGGCC TAGAAAACAA AAGAACAGCC
AAGAGGAACT CAAAAGCCAA AGGAAAAAGT GATTCTGAAG ATGGGGAGAA GGAAAATGAA
AAATCTAAAA CTTCAGATTC TTCAAATGAC GAATCTAGTT CAATAGAAGA CAGTTCTTCC
GATTCTGAAT CAGAGTCAGA ACCTGAAAGT GAATCTGAAT CCAGAAGAGT CACTAAGGAG
AAAGAAAAGA AAACAAAGCA AGATAGAACT CCTCTTACCA AAGATGTTTC ACTTCTAGAT
CTGGATGATT TTAACCCAGT ATCCACTCCA GTTGCACTTC CCACACCAGC TCTTTCTCCA
AGTTTGATGG CTGATCTTGA AGGTTTACAC TTGTCAACTT CCTCTTCAGT CATCAGTGAA
AGAAAAGCAC TCCATCTAAA GTTGGGCAAA AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248676.1
CDS149..2764
Misc Feature(1)284..1891
Misc Feature(2)order(434..439,446..448,455..460,545..550,557..559,566..571,578..580,650..655,662..664,671..676,683..685,761..766,773..775,782..787)
Misc Feature(3)494..580
Misc Feature(4)530..>901
Misc Feature(5)596..688
Misc Feature(6)710..787
Misc Feature(7)<2333..>2440
Misc Feature(8)2588..>2728
Translation

Target ORF information:

RefSeq Version XM_005248619.4
Organism Homo sapiens(Human)
Definition Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248619.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
ATGTCCAGCA ATAGTTTTCC TTACAATGAG CAGTCCGGAG GAGGGGAGGC GACGGAGCTG 
GGTCAGGAGG CGACCTCAAC CATTTCCCCC TCGGGGGCCT TCGGCCTCTT TAGCAGCGAT
TTGAAGAAGA ATGAAGATCT AAAGCAAATG TTAGAGAGCA ACAAAGATTC TGCTAAACTG
GATGCTATGA AGCGGATTGT TGGGATGATT GCAAAAGGGA AAAATGCATC TGAACTGTTT
CCTGCTGTTG TGAAGAATGT GGCCAGTAAA AATATTGAGA TCAAGAAGTT GGTATATGTT
TACCTGGTTC GATATGCTGA AGAACAGCAG GATCTTGCAC TCCTGTCCAT AAGCACTTTT
CAGCGAGCTC TGAAGGACCC AAACCAACTA ATTCGTGCAA GCGCTTTGAG AGTTCTGTCA
AGTATTAGAG TGCCAATTAT TGTACCTATC ATGATGCTTG CTATTAAGGA AGCTTCTGCT
GACTTATCAC CATATGTTAG GAAGAATGCA GCCCATGCAA TACAAAAATT ATACAGCCTT
GATCCAGAGC AGAAGGAAAT GTTAATTGAA GTAATTGAAA AACTTCTGAA AGATAAAAGC
ACATTGGTAG CTGGCAGTGT TGTGATGGCT TTTGAAGAAG TATGCCCGGA CAGAATAGAT
CTGATTCATA AAAATTACCG CAAGCTATGT AACTTACTAG TGGATGTTGA AGAGTGGGGG
CAGGTTGTCA TAATCCACAT GCTAACTCGA TATGCTCGGA CACAGTTTGT CAGCCCTTGG
AAAGAGGGTG ATGAATTAGA AGACAATGGA AAGAATTTCT ACGAATCTGA TGATGATCAG
AAGGAAAAGA CTGACAAAAA GAAGAAGCCG TATACTATGG ATCCAGATCA TAGACTCTTA
ATTAGAAATA CAAAGCCTTT GCTTCAGAGC AGGAATGCTG CGGTGGTTAT GGCAGTTGCT
CAGCTGTATT GGCACATATC ACCAAAATCT GAAGCTGGCA TAATTTCTAA ATCACTAGTG
CGTTTACTTC GTAGCAATAG GGAGGTGCAG TATATTGTCC TACAAAATAT AGCAACTATG
TCAATTCAAA GAAAGGGGAT GTTTGAACCT TATCTGAAGA GTTTCTATGT TAGGTCAACT
GATCCAACTA TGATCAAGAC ACTGAAGCTT GAAATTTTGA CAAACTTGGC AAATGAAGCC
AACATATCAA CTCTTCTTCG AGAATTTCAG ACCTATGTGA AAAGCCAGGA TAAACAATTT
GCAGCAGCCA CTATTCAGAC TATAGGCAGA TGTGCAACCA ACATCTTGGA AGTCACTGAC
ACGTGCCTCA ATGGCTTGGT CTGTCTGCTG TCCAACAGGG ATGAAATAGT TGTTGCTGAA
AGTGTGGTTG TTATAAAGAA ATTACTGCAA ATGCAACCTG CACAACATGG TGAAATTATT
AAACATATGG CCAAACTCCT GGACAGTATC ACTGTTCCTG TTGCTAGAGC AAGTATTCTT
TGGCTAATTG GAGAAAACTG TGAACGAGTT CCTAAAATTG CCCCTGATGT TTTGAGGAAG
ATGGCTAAAA GCTTCACTAG TGAAGATGAT CTGGTAAAAC TGCAGATATT AAATCTGGGA
GCAAAATTGT ATTTAACCAA CTCCAAACAG ACAAAATTGC TTACCCAGTA CATATTAAAT
CTCGGCAAGT ATGATCAAAA CTACGACATC AGAGACCGTA CAAGATTTAT TAGGCAGCTT
ATTGTTCCGA ATGTAAAGAG TGGAGCTTTA AGTAAATATG CCAAAAAAAT ATTCCTAGCA
CAAAAGCCTG CACCACTGCT TGAGTCTCCT TTTAAAGATA GAGATCATTT CCAGCTTGGC
ACCTTATCTC ATACTCTCAA CATTAAAGCT ACTGGGTACC TGGAATTATC TAATTGGCCA
GAGGTGGCGC CCGACCCATC AGTTCGAAAT GTAGAAGTAA TAGAGTTGGC AAAAGAATGG
ACCCCAGCAG GAAAAGCAAA GCAAGAGAAT TCTGCTAAGA AGTTTTATTC TGAATCTGAG
GAAGAGGAGG ACTCTTCTGA TAGTAGCAGT GACAGTGAGA GTGAATCTGG AAGTGAAAGT
GGAGAACAAG GCGAAAGTGG GGAGGAAGGA GACAGCAATG AGGACAGCAG TGAGGACTCC
TCCAGTGAGC AGGACAGTGA GAGTGGACGG GAGTCAGGCC TAGAAAACAA AAGAACAGCC
AAGAGGAACT CAAAAGCCAA AGGAAAAAGT GATTCTGAAG ATGGGGAGAA GGAAAATGAA
AAATCTAAAA CTTCAGATTC TTCAAATGAC GAATCTAGTT CAATAGAAGA CAGTTCTTCC
GATTCTGAAT CAGAGTCAGA ACCTGAAAGT GAATCTGAAT CCAGAAGAGT CACTAAGGAG
AAAGAAAAGA AAACAAAGCA AGATAGAACT CCTCTTACCA AAGATGTTTC ACTTCTAGAT
CTGGATGATT TTAACCCAGT ATCCACTCCA GTTGCACTTC CCACACCAGC TCTTTCTCCA
AGTTTGATGG CTGATCTTGA AGGTTTACAC TTGTCAACTT CCTCTTCAGT CATCAGTGAA
AGAAAAGCAC TCCATCTAAA GTTGGGCAAA AAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu96173
Clone ID Related Accession (Same CDS sequence) XM_017010001.1
Accession Version XM_017010001.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3015bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product AP-3 complex subunit beta-1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGTTAGAGA GCAACAAAGA TTCTGCTAAA CTGGATGCTA TGAAGCGGAT TGTTGGGATG 
ATTGCAAAAG GGAAAAATGC ATCTGAACTG TTTCCTGCTG TTGTGAAGAA TGTGGCCAGT
AAAAATATTG AGATCAAGAA GTTGGTATAT GTTTACCTGG TTCGATATGC TGAAGAACAG
CAGGATCTTG CACTCCTGTC CATAAGCACT TTTCAGCGAG CTCTGAAGGA CCCAAACCAA
CTAATTCGTG CAAGCGCTTT GAGAGTTCTG TCAAGTATTA GAGTGCCAAT TATTGTACCT
ATCATGATGC TTGCTATTAA GGAAGCTTCT GCTGACTTAT CACCATATGT TAGGAAGAAT
GCAGCCCATG CAATACAAAA ATTATACAGC CTTGATCCAG AGCAGAAGGA AATGTTAATT
GAAGTAATTG AAAAACTTCT GAAAGATAAA AGCACATTGG TAGCTGGCAG TGTTGTGATG
GCTTTTGAAG AAGTATGCCC GGACAGAATA GATCTGATTC ATAAAAATTA CCGCAAGCTA
TGTAACTTAC TAGTGGATGT TGAAGAGTGG GGGCAGGTTG TCATAATCCA CATGCTAACT
CGATATGCTC GGACACAGTT TGTCAGCCCT TGGAAAGAGG GTGATGAATT AGAAGACAAT
GGAAAGAATT TCTACGAATC TGATGATGAT CAGAAGGAAA AGACTGACAA AAAGAAGAAG
CCGTATACTA TGGATCCAGA TCATAGACTC TTAATTAGAA ATACAAAGCC TTTGCTTCAG
AGCAGGAATG CTGCGGTGGT TATGGCAGTT GCTCAGCTGT ATTGGCACAT ATCACCAAAA
TCTGAAGCTG GCATAATTTC TAAATCACTA GTGCGTTTAC TTCGTAGCAA TAGGGAGGTG
CAGTATATTG TCCTACAAAA TATAGCAACT ATGTCAATTC AAAGAAAGGG GATGTTTGAA
CCTTATCTGA AGAGTTTCTA TGTTAGGTCA ACTGATCCAA CTATGATCAA GACACTGAAG
CTTGAAATTT TGACAAACTT GGCAAATGAA GCCAACATAT CAACTCTTCT TCGAGAATTT
CAGACCTATG TGAAAAGCCA GGATAAACAA TTTGCAGCAG CCACTATTCA GACTATAGGC
AGATGTGCAA CCAACATCTT GGAAGTCACT GACACGTGCC TCAATGGCTT GGTCTGTCTG
CTGTCCAACA GGGATGAAAT AGTTGTTGCT GAAAGTGTGG TTGTTATAAA GAAATTACTG
CAAATGCAAC CTGCACAACA TGGTGAAATT ATTAAACATA TGGCCAAACT CCTGGACAGT
ATCACTGTTC CTGTTGCTAG AGCAAGTATT CTTTGGCTAA TTGGAGAAAA CTGTGAACGA
GTTCCTAAAA TTGCCCCTGA TGTTTTGAGG AAGATGGCTA AAAGCTTCAC TAGTGAAGAT
GATCTGGTAA AACTGCAGAT ATTAAATCTG GGAGCAAAAT TGTATTTAAC CAACTCCAAA
CAGACAAAAT TGCTTACCCA GTACATATTA AATCTCGGCA AGTATGATCA AAACTACGAC
ATCAGAGACC GTACAAGATT TATTAGGCAG CTTATTGTTC CGAATGTAAA GAGTGGAGCT
TTAAGTAAAT ATGCCAAAAA AATATTCCTA GCACAAAAGC CTGCACCACT GCTTGAGTCT
CCTTTTAAAG ATAGAGATCA TTTCCAGCTT GGCACCTTAT CTCATACTCT CAACATTAAA
GCTACTGGGT ACCTGGAATT ATCTAATTGG CCAGAGGTGG CGCCCGACCC ATCAGTTCGA
AATGTAGAAG TAATAGAGTT GGCAAAAGAA TGGACCCCAG CAGGAAAAGC AAAGCAAGAG
AATTCTGCTA AGAAGTTTTA TTCTGAATCT GAGGAAGAGG AGGACTCTTC TGATAGTAGC
AGTGACAGTG AGAGTGAATC TGGAAGTGAA AGTGGAGAAC AAGGCGAAAG TGGGGAGGAA
GGAGACAGCA ATGAGGACAG CAGTGAGGAC TCCTCCAGTG AGCAGGACAG TGAGAGTGGA
CGGGAGTCAG GCCTAGAAAA CAAAAGAACA GCCAAGAGGA ACTCAAAAGC CAAAGGAAAA
AGTGATTCTG AAGATGGGGA GAAGGAAAAT GAAAAATCTA AAACTTCAGA TTCTTCAAAT
GACGAATCTA GTTCAATAGA AGACAGTTCT TCCGATTCTG AATCAGAGTC AGAACCTGAA
AGTGAATCTG AATCCAGAAG AGTCACTAAG GAGAAAGAAA AGAAAACAAA GCAAGATAGA
ACTCCTCTTA CCAAAGATGT TTCACTTCTA GATCTGGATG ATTTTAACCC AGTATCCACT
CCAGTTGCAC TTCCCACACC AGCTCTTTCT CCAAGTTTGA TGGCTGATCT TGAAGGTTTA
CACTTGTCAA CTTCCTCTTC AGTCATCAGT GTCAGTACTC CTGCATTTGT ACCAACGAAA
ACTCACGTGC TGCTTCATCG AATGAGTGGA AAAGGACTAG CTGCCCATTA TTTCTTTCCA
AGACAGCCTT GCATTTTTGG TGATAAGATG GTCTCTATAC AAATAACACT GAATAACACT
ACTGATCGAA AGATAGAAAA TATCCACATA GGGGAAAAAA AACTTCCTAT AGGCATGAAA
ATGCATGTTT TTAATCCAAT AGACTCTCTT GAGCCTGAGG GATCCATTAC AGTTTCAATG
GGTATTGACT TTTGTGATTC TACTCAGACT GCCAGTTTCC AGTTGTGTAC CAAGGATGAT
TGCTTCAATG TTAATATTCA GCCACCTGTT GGAGAACTGC TTTTACCTGT GGCCATGTCA
GAGAAAGATT TTAAGAAAGA GCAAGGAGTG CTAACAGGAA TGAATGAAAC TTCTGCTGTA
ATCATTGCTG CACCACAGAA TTTCACTCCC TCTGTGATCT TTCAGAAGGT TGTAAATGTA
GCCAATGTAG GTGCAGTCCC TTCTGGCCAG GATAATATAC ACAGATATGA GGAGTTAAAT
TTTGAAGCTC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016865490.1
CDS76..3090
Translation

Target ORF information:

RefSeq Version XM_017010001.1
Organism Homo sapiens(Human)
Definition Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017010001.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGTTAGAGA GCAACAAAGA TTCTGCTAAA CTGGATGCTA TGAAGCGGAT TGTTGGGATG 
ATTGCAAAAG GGAAAAATGC ATCTGAACTG TTTCCTGCTG TTGTGAAGAA TGTGGCCAGT
AAAAATATTG AGATCAAGAA GTTGGTATAT GTTTACCTGG TTCGATATGC TGAAGAACAG
CAGGATCTTG CACTCCTGTC CATAAGCACT TTTCAGCGAG CTCTGAAGGA CCCAAACCAA
CTAATTCGTG CAAGCGCTTT GAGAGTTCTG TCAAGTATTA GAGTGCCAAT TATTGTACCT
ATCATGATGC TTGCTATTAA GGAAGCTTCT GCTGACTTAT CACCATATGT TAGGAAGAAT
GCAGCCCATG CAATACAAAA ATTATACAGC CTTGATCCAG AGCAGAAGGA AATGTTAATT
GAAGTAATTG AAAAACTTCT GAAAGATAAA AGCACATTGG TAGCTGGCAG TGTTGTGATG
GCTTTTGAAG AAGTATGCCC GGACAGAATA GATCTGATTC ATAAAAATTA CCGCAAGCTA
TGTAACTTAC TAGTGGATGT TGAAGAGTGG GGGCAGGTTG TCATAATCCA CATGCTAACT
CGATATGCTC GGACACAGTT TGTCAGCCCT TGGAAAGAGG GTGATGAATT AGAAGACAAT
GGAAAGAATT TCTACGAATC TGATGATGAT CAGAAGGAAA AGACTGACAA AAAGAAGAAG
CCGTATACTA TGGATCCAGA TCATAGACTC TTAATTAGAA ATACAAAGCC TTTGCTTCAG
AGCAGGAATG CTGCGGTGGT TATGGCAGTT GCTCAGCTGT ATTGGCACAT ATCACCAAAA
TCTGAAGCTG GCATAATTTC TAAATCACTA GTGCGTTTAC TTCGTAGCAA TAGGGAGGTG
CAGTATATTG TCCTACAAAA TATAGCAACT ATGTCAATTC AAAGAAAGGG GATGTTTGAA
CCTTATCTGA AGAGTTTCTA TGTTAGGTCA ACTGATCCAA CTATGATCAA GACACTGAAG
CTTGAAATTT TGACAAACTT GGCAAATGAA GCCAACATAT CAACTCTTCT TCGAGAATTT
CAGACCTATG TGAAAAGCCA GGATAAACAA TTTGCAGCAG CCACTATTCA GACTATAGGC
AGATGTGCAA CCAACATCTT GGAAGTCACT GACACGTGCC TCAATGGCTT GGTCTGTCTG
CTGTCCAACA GGGATGAAAT AGTTGTTGCT GAAAGTGTGG TTGTTATAAA GAAATTACTG
CAAATGCAAC CTGCACAACA TGGTGAAATT ATTAAACATA TGGCCAAACT CCTGGACAGT
ATCACTGTTC CTGTTGCTAG AGCAAGTATT CTTTGGCTAA TTGGAGAAAA CTGTGAACGA
GTTCCTAAAA TTGCCCCTGA TGTTTTGAGG AAGATGGCTA AAAGCTTCAC TAGTGAAGAT
GATCTGGTAA AACTGCAGAT ATTAAATCTG GGAGCAAAAT TGTATTTAAC CAACTCCAAA
CAGACAAAAT TGCTTACCCA GTACATATTA AATCTCGGCA AGTATGATCA AAACTACGAC
ATCAGAGACC GTACAAGATT TATTAGGCAG CTTATTGTTC CGAATGTAAA GAGTGGAGCT
TTAAGTAAAT ATGCCAAAAA AATATTCCTA GCACAAAAGC CTGCACCACT GCTTGAGTCT
CCTTTTAAAG ATAGAGATCA TTTCCAGCTT GGCACCTTAT CTCATACTCT CAACATTAAA
GCTACTGGGT ACCTGGAATT ATCTAATTGG CCAGAGGTGG CGCCCGACCC ATCAGTTCGA
AATGTAGAAG TAATAGAGTT GGCAAAAGAA TGGACCCCAG CAGGAAAAGC AAAGCAAGAG
AATTCTGCTA AGAAGTTTTA TTCTGAATCT GAGGAAGAGG AGGACTCTTC TGATAGTAGC
AGTGACAGTG AGAGTGAATC TGGAAGTGAA AGTGGAGAAC AAGGCGAAAG TGGGGAGGAA
GGAGACAGCA ATGAGGACAG CAGTGAGGAC TCCTCCAGTG AGCAGGACAG TGAGAGTGGA
CGGGAGTCAG GCCTAGAAAA CAAAAGAACA GCCAAGAGGA ACTCAAAAGC CAAAGGAAAA
AGTGATTCTG AAGATGGGGA GAAGGAAAAT GAAAAATCTA AAACTTCAGA TTCTTCAAAT
GACGAATCTA GTTCAATAGA AGACAGTTCT TCCGATTCTG AATCAGAGTC AGAACCTGAA
AGTGAATCTG AATCCAGAAG AGTCACTAAG GAGAAAGAAA AGAAAACAAA GCAAGATAGA
ACTCCTCTTA CCAAAGATGT TTCACTTCTA GATCTGGATG ATTTTAACCC AGTATCCACT
CCAGTTGCAC TTCCCACACC AGCTCTTTCT CCAAGTTTGA TGGCTGATCT TGAAGGTTTA
CACTTGTCAA CTTCCTCTTC AGTCATCAGT GTCAGTACTC CTGCATTTGT ACCAACGAAA
ACTCACGTGC TGCTTCATCG AATGAGTGGA AAAGGACTAG CTGCCCATTA TTTCTTTCCA
AGACAGCCTT GCATTTTTGG TGATAAGATG GTCTCTATAC AAATAACACT GAATAACACT
ACTGATCGAA AGATAGAAAA TATCCACATA GGGGAAAAAA AACTTCCTAT AGGCATGAAA
ATGCATGTTT TTAATCCAAT AGACTCTCTT GAGCCTGAGG GATCCATTAC AGTTTCAATG
GGTATTGACT TTTGTGATTC TACTCAGACT GCCAGTTTCC AGTTGTGTAC CAAGGATGAT
TGCTTCAATG TTAATATTCA GCCACCTGTT GGAGAACTGC TTTTACCTGT GGCCATGTCA
GAGAAAGATT TTAAGAAAGA GCAAGGAGTG CTAACAGGAA TGAATGAAAC TTCTGCTGTA
ATCATTGCTG CACCACAGAA TTTCACTCCC TCTGTGATCT TTCAGAAGGT TGTAAATGTA
GCCAATGTAG GTGCAGTCCC TTCTGGCCAG GATAATATAC ACAGATATGA GGAGTTAAAT
TTTGAAGCTC TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21104
Clone ID Related Accession (Same CDS sequence) NM_003664.4
Accession Version NM_003664.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3285bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product AP-3 complex subunit beta-1 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA889915.1, BC038444.1 and AC112197.3. This sequence is a reference standard in the RefSeqGene project. On Nov 17, 2012 this sequence version replaced NM_003664.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038444.1, U81504.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCAGCA ATAGTTTTCC TTACAATGAG CAGTCCGGAG GAGGGGAGGC GACGGAGCTG 
GGTCAGGAGG CGACCTCAAC CATTTCCCCC TCGGGGGCCT TCGGCCTCTT TAGCAGCGAT
TTGAAGAAGA ATGAAGATCT AAAGCAAATG TTAGAGAGCA ACAAAGATTC TGCTAAACTG
GATGCTATGA AGCGGATTGT TGGGATGATT GCAAAAGGGA AAAATGCATC TGAACTGTTT
CCTGCTGTTG TGAAGAATGT GGCCAGTAAA AATATTGAGA TCAAGAAGTT GGTATATGTT
TACCTGGTTC GATATGCTGA AGAACAGCAG GATCTTGCAC TCCTGTCCAT AAGCACTTTT
CAGCGAGCTC TGAAGGACCC AAACCAACTA ATTCGTGCAA GCGCTTTGAG AGTTCTGTCA
AGTATTAGAG TGCCAATTAT TGTACCTATC ATGATGCTTG CTATTAAGGA AGCTTCTGCT
GACTTATCAC CATATGTTAG GAAGAATGCA GCCCATGCAA TACAAAAATT ATACAGCCTT
GATCCAGAGC AGAAGGAAAT GTTAATTGAA GTAATTGAAA AACTTCTGAA AGATAAAAGC
ACATTGGTAG CTGGCAGTGT TGTGATGGCT TTTGAAGAAG TATGCCCGGA CAGAATAGAT
CTGATTCATA AAAATTACCG CAAGCTATGT AACTTACTAG TGGATGTTGA AGAGTGGGGG
CAGGTTGTCA TAATCCACAT GCTAACTCGA TATGCTCGGA CACAGTTTGT CAGCCCTTGG
AAAGAGGGTG ATGAATTAGA AGACAATGGA AAGAATTTCT ACGAATCTGA TGATGATCAG
AAGGAAAAGA CTGACAAAAA GAAGAAGCCG TATACTATGG ATCCAGATCA TAGACTCTTA
ATTAGAAATA CAAAGCCTTT GCTTCAGAGC AGGAATGCTG CGGTGGTTAT GGCAGTTGCT
CAGCTGTATT GGCACATATC ACCAAAATCT GAAGCTGGCA TAATTTCTAA ATCACTAGTG
CGTTTACTTC GTAGCAATAG GGAGGTGCAG TATATTGTCC TACAAAATAT AGCAACTATG
TCAATTCAAA GAAAGGGGAT GTTTGAACCT TATCTGAAGA GTTTCTATGT TAGGTCAACT
GATCCAACTA TGATCAAGAC ACTGAAGCTT GAAATTTTGA CAAACTTGGC AAATGAAGCC
AACATATCAA CTCTTCTTCG AGAATTTCAG ACCTATGTGA AAAGCCAGGA TAAACAATTT
GCAGCAGCCA CTATTCAGAC TATAGGCAGA TGTGCAACCA ACATCTTGGA AGTCACTGAC
ACGTGCCTCA ATGGCTTGGT CTGTCTGCTG TCCAACAGGG ATGAAATAGT TGTTGCTGAA
AGTGTGGTTG TTATAAAGAA ATTACTGCAA ATGCAACCTG CACAACATGG TGAAATTATT
AAACATATGG CCAAACTCCT GGACAGTATC ACTGTTCCTG TTGCTAGAGC AAGTATTCTT
TGGCTAATTG GAGAAAACTG TGAACGAGTT CCTAAAATTG CCCCTGATGT TTTGAGGAAG
ATGGCTAAAA GCTTCACTAG TGAAGATGAT CTGGTAAAAC TGCAGATATT AAATCTGGGA
GCAAAATTGT ATTTAACCAA CTCCAAACAG ACAAAATTGC TTACCCAGTA CATATTAAAT
CTCGGCAAGT ATGATCAAAA CTACGACATC AGAGACCGTA CAAGATTTAT TAGGCAGCTT
ATTGTTCCGA ATGTAAAGAG TGGAGCTTTA AGTAAATATG CCAAAAAAAT ATTCCTAGCA
CAAAAGCCTG CACCACTGCT TGAGTCTCCT TTTAAAGATA GAGATCATTT CCAGCTTGGC
ACCTTATCTC ATACTCTCAA CATTAAAGCT ACTGGGTACC TGGAATTATC TAATTGGCCA
GAGGTGGCGC CCGACCCATC AGTTCGAAAT GTAGAAGTAA TAGAGTTGGC AAAAGAATGG
ACCCCAGCAG GAAAAGCAAA GCAAGAGAAT TCTGCTAAGA AGTTTTATTC TGAATCTGAG
GAAGAGGAGG ACTCTTCTGA TAGTAGCAGT GACAGTGAGA GTGAATCTGG AAGTGAAAGT
GGAGAACAAG GCGAAAGTGG GGAGGAAGGA GACAGCAATG AGGACAGCAG TGAGGACTCC
TCCAGTGAGC AGGACAGTGA GAGTGGACGG GAGTCAGGCC TAGAAAACAA AAGAACAGCC
AAGAGGAACT CAAAAGCCAA AGGAAAAAGT GATTCTGAAG ATGGGGAGAA GGAAAATGAA
AAATCTAAAA CTTCAGATTC TTCAAATGAC GAATCTAGTT CAATAGAAGA CAGTTCTTCC
GATTCTGAAT CAGAGTCAGA ACCTGAAAGT GAATCTGAAT CCAGAAGAGT CACTAAGGAG
AAAGAAAAGA AAACAAAGCA AGATAGAACT CCTCTTACCA AAGATGTTTC ACTTCTAGAT
CTGGATGATT TTAACCCAGT ATCCACTCCA GTTGCACTTC CCACACCAGC TCTTTCTCCA
AGTTTGATGG CTGATCTTGA AGGTTTACAC TTGTCAACTT CCTCTTCAGT CATCAGTGTC
AGTACTCCTG CATTTGTACC AACGAAAACT CACGTGCTGC TTCATCGAAT GAGTGGAAAA
GGACTAGCTG CCCATTATTT CTTTCCAAGA CAGCCTTGCA TTTTTGGTGA TAAGATGGTC
TCTATACAAA TAACACTGAA TAACACTACT GATCGAAAGA TAGAAAATAT CCACATAGGG
GAAAAAAAAC TTCCTATAGG CATGAAAATG CATGTTTTTA ATCCAATAGA CTCTCTTGAG
CCTGAGGGAT CCATTACAGT TTCAATGGGT ATTGACTTTT GTGATTCTAC TCAGACTGCC
AGTTTCCAGT TGTGTACCAA GGATGATTGC TTCAATGTTA ATATTCAGCC ACCTGTTGGA
GAACTGCTTT TACCTGTGGC CATGTCAGAG AAAGATTTTA AGAAAGAGCA AGGAGTGCTA
ACAGGAATGA ATGAAACTTC TGCTGTAATC ATTGCTGCAC CACAGAATTT CACTCCCTCT
GTGATCTTTC AGAAGGTTGT AAATGTAGCC AATGTAGGTG CAGTCCCTTC TGGCCAGGAT
AATATACACA GGTTTGCAGC TAAAACTGTG CACAGTGGGT CATTGATGCT AGTCACAGTG
GAACTGAAGG AAGGCTCTAC AGCCCAGCTT ATCATAAACA CTGAGAAAAC TGTGATTGGC
TCTGTTCTGC TGCGGGAACT GAAGCCTGTC CTGTCTCAGG GGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003655.3
CDS177..3461
Misc Feature(1)48..50
Misc Feature(2)312..1919
Misc Feature(3)order(462..467,474..476,483..488,573..578,585..587,594..599,606..608,678..683,690..692,699..704,711..713,789..794,801..803,810..815)
Misc Feature(4)522..608
Misc Feature(5)558..>929
Misc Feature(6)624..716
Misc Feature(7)738..815
Misc Feature(8)1002..1004
Misc Feature(9)1002..1004
Misc Feature(10)2001..2003
Misc Feature(11)2001..2003
Misc Feature(12)<2361..>2468
Misc Feature(13)2424..2426
Misc Feature(14)2430..2432
Misc Feature(15)2616..3050
Exon (1)1..304
Exon (2)305..380
Exon (3)381..455
Exon (4)456..551
Exon (5)552..712
Exon (6)713..779
Exon (7)780..962
Exon (8)963..1118
Exon (9)1119..1216
Exon (10)1217..1271
Exon (11)1272..1343
Exon (12)1344..1406
Exon (13)1407..1539
Exon (14)1540..1649
Exon (15)1650..1826
Exon (16)1827..2013
Exon (17)2014..2144
Exon (18)2145..2253
Exon (19)2254..2425
Exon (20)2426..2573
Exon (21)2574..2646
Exon (22)2647..2753
Exon (23)2754..2985
Exon (24)2986..3070
Exon (25)3071..3168
Exon (26)3169..3307
Exon (27)3308..4037
Translation

Target ORF information:

RefSeq Version NM_003664.4
Organism Homo sapiens(Human)
Definition Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003664.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCAGCA ATAGTTTTCC TTACAATGAG CAGTCCGGAG GAGGGGAGGC GACGGAGCTG 
GGTCAGGAGG CGACCTCAAC CATTTCCCCC TCGGGGGCCT TCGGCCTCTT TAGCAGCGAT
TTGAAGAAGA ATGAAGATCT AAAGCAAATG TTAGAGAGCA ACAAAGATTC TGCTAAACTG
GATGCTATGA AGCGGATTGT TGGGATGATT GCAAAAGGGA AAAATGCATC TGAACTGTTT
CCTGCTGTTG TGAAGAATGT GGCCAGTAAA AATATTGAGA TCAAGAAGTT GGTATATGTT
TACCTGGTTC GATATGCTGA AGAACAGCAG GATCTTGCAC TCCTGTCCAT AAGCACTTTT
CAGCGAGCTC TGAAGGACCC AAACCAACTA ATTCGTGCAA GCGCTTTGAG AGTTCTGTCA
AGTATTAGAG TGCCAATTAT TGTACCTATC ATGATGCTTG CTATTAAGGA AGCTTCTGCT
GACTTATCAC CATATGTTAG GAAGAATGCA GCCCATGCAA TACAAAAATT ATACAGCCTT
GATCCAGAGC AGAAGGAAAT GTTAATTGAA GTAATTGAAA AACTTCTGAA AGATAAAAGC
ACATTGGTAG CTGGCAGTGT TGTGATGGCT TTTGAAGAAG TATGCCCGGA CAGAATAGAT
CTGATTCATA AAAATTACCG CAAGCTATGT AACTTACTAG TGGATGTTGA AGAGTGGGGG
CAGGTTGTCA TAATCCACAT GCTAACTCGA TATGCTCGGA CACAGTTTGT CAGCCCTTGG
AAAGAGGGTG ATGAATTAGA AGACAATGGA AAGAATTTCT ACGAATCTGA TGATGATCAG
AAGGAAAAGA CTGACAAAAA GAAGAAGCCG TATACTATGG ATCCAGATCA TAGACTCTTA
ATTAGAAATA CAAAGCCTTT GCTTCAGAGC AGGAATGCTG CGGTGGTTAT GGCAGTTGCT
CAGCTGTATT GGCACATATC ACCAAAATCT GAAGCTGGCA TAATTTCTAA ATCACTAGTG
CGTTTACTTC GTAGCAATAG GGAGGTGCAG TATATTGTCC TACAAAATAT AGCAACTATG
TCAATTCAAA GAAAGGGGAT GTTTGAACCT TATCTGAAGA GTTTCTATGT TAGGTCAACT
GATCCAACTA TGATCAAGAC ACTGAAGCTT GAAATTTTGA CAAACTTGGC AAATGAAGCC
AACATATCAA CTCTTCTTCG AGAATTTCAG ACCTATGTGA AAAGCCAGGA TAAACAATTT
GCAGCAGCCA CTATTCAGAC TATAGGCAGA TGTGCAACCA ACATCTTGGA AGTCACTGAC
ACGTGCCTCA ATGGCTTGGT CTGTCTGCTG TCCAACAGGG ATGAAATAGT TGTTGCTGAA
AGTGTGGTTG TTATAAAGAA ATTACTGCAA ATGCAACCTG CACAACATGG TGAAATTATT
AAACATATGG CCAAACTCCT GGACAGTATC ACTGTTCCTG TTGCTAGAGC AAGTATTCTT
TGGCTAATTG GAGAAAACTG TGAACGAGTT CCTAAAATTG CCCCTGATGT TTTGAGGAAG
ATGGCTAAAA GCTTCACTAG TGAAGATGAT CTGGTAAAAC TGCAGATATT AAATCTGGGA
GCAAAATTGT ATTTAACCAA CTCCAAACAG ACAAAATTGC TTACCCAGTA CATATTAAAT
CTCGGCAAGT ATGATCAAAA CTACGACATC AGAGACCGTA CAAGATTTAT TAGGCAGCTT
ATTGTTCCGA ATGTAAAGAG TGGAGCTTTA AGTAAATATG CCAAAAAAAT ATTCCTAGCA
CAAAAGCCTG CACCACTGCT TGAGTCTCCT TTTAAAGATA GAGATCATTT CCAGCTTGGC
ACCTTATCTC ATACTCTCAA CATTAAAGCT ACTGGGTACC TGGAATTATC TAATTGGCCA
GAGGTGGCGC CCGACCCATC AGTTCGAAAT GTAGAAGTAA TAGAGTTGGC AAAAGAATGG
ACCCCAGCAG GAAAAGCAAA GCAAGAGAAT TCTGCTAAGA AGTTTTATTC TGAATCTGAG
GAAGAGGAGG ACTCTTCTGA TAGTAGCAGT GACAGTGAGA GTGAATCTGG AAGTGAAAGT
GGAGAACAAG GCGAAAGTGG GGAGGAAGGA GACAGCAATG AGGACAGCAG TGAGGACTCC
TCCAGTGAGC AGGACAGTGA GAGTGGACGG GAGTCAGGCC TAGAAAACAA AAGAACAGCC
AAGAGGAACT CAAAAGCCAA AGGAAAAAGT GATTCTGAAG ATGGGGAGAA GGAAAATGAA
AAATCTAAAA CTTCAGATTC TTCAAATGAC GAATCTAGTT CAATAGAAGA CAGTTCTTCC
GATTCTGAAT CAGAGTCAGA ACCTGAAAGT GAATCTGAAT CCAGAAGAGT CACTAAGGAG
AAAGAAAAGA AAACAAAGCA AGATAGAACT CCTCTTACCA AAGATGTTTC ACTTCTAGAT
CTGGATGATT TTAACCCAGT ATCCACTCCA GTTGCACTTC CCACACCAGC TCTTTCTCCA
AGTTTGATGG CTGATCTTGA AGGTTTACAC TTGTCAACTT CCTCTTCAGT CATCAGTGTC
AGTACTCCTG CATTTGTACC AACGAAAACT CACGTGCTGC TTCATCGAAT GAGTGGAAAA
GGACTAGCTG CCCATTATTT CTTTCCAAGA CAGCCTTGCA TTTTTGGTGA TAAGATGGTC
TCTATACAAA TAACACTGAA TAACACTACT GATCGAAAGA TAGAAAATAT CCACATAGGG
GAAAAAAAAC TTCCTATAGG CATGAAAATG CATGTTTTTA ATCCAATAGA CTCTCTTGAG
CCTGAGGGAT CCATTACAGT TTCAATGGGT ATTGACTTTT GTGATTCTAC TCAGACTGCC
AGTTTCCAGT TGTGTACCAA GGATGATTGC TTCAATGTTA ATATTCAGCC ACCTGTTGGA
GAACTGCTTT TACCTGTGGC CATGTCAGAG AAAGATTTTA AGAAAGAGCA AGGAGTGCTA
ACAGGAATGA ATGAAACTTC TGCTGTAATC ATTGCTGCAC CACAGAATTT CACTCCCTCT
GTGATCTTTC AGAAGGTTGT AAATGTAGCC AATGTAGGTG CAGTCCCTTC TGGCCAGGAT
AATATACACA GGTTTGCAGC TAAAACTGTG CACAGTGGGT CATTGATGCT AGTCACAGTG
GAACTGAAGG AAGGCTCTAC AGCCCAGCTT ATCATAAACA CTGAGAAAAC TGTGATTGGC
TCTGTTCTGC TGCGGGAACT GAAGCCTGTC CTGTCTCAGG GGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21364
Clone ID Related Accession (Same CDS sequence) NM_001271769.1
Accession Version NM_001271769.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product AP-3 complex subunit beta-1 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA889915.1, BX538041.1, BC038444.1 and AC112197.3. Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of the first exon compared to variant 1. This transcript initiates translation at an alternate start codon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX538041.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGTTAGAGA GCAACAAAGA TTCTGCTAAA CTGGATGCTA TGAAGCGGAT TGTTGGGATG 
ATTGCAAAAG GGAAAAATGC ATCTGAACTG TTTCCTGCTG TTGTGAAGAA TGTGGCCAGT
AAAAATATTG AGATCAAGAA GTTGGTATAT GTTTACCTGG TTCGATATGC TGAAGAACAG
CAGGATCTTG CACTCCTGTC CATAAGCACT TTTCAGCGAG CTCTGAAGGA CCCAAACCAA
CTAATTCGTG CAAGCGCTTT GAGAGTTCTG TCAAGTATTA GAGTGCCAAT TATTGTACCT
ATCATGATGC TTGCTATTAA GGAAGCTTCT GCTGACTTAT CACCATATGT TAGGAAGAAT
GCAGCCCATG CAATACAAAA ATTATACAGC CTTGATCCAG AGCAGAAGGA AATGTTAATT
GAAGTAATTG AAAAACTTCT GAAAGATAAA AGCACATTGG TAGCTGGCAG TGTTGTGATG
GCTTTTGAAG AAGTATGCCC GGACAGAATA GATCTGATTC ATAAAAATTA CCGCAAGCTA
TGTAACTTAC TAGTGGATGT TGAAGAGTGG GGGCAGGTTG TCATAATCCA CATGCTAACT
CGATATGCTC GGACACAGTT TGTCAGCCCT TGGAAAGAGG GTGATGAATT AGAAGACAAT
GGAAAGAATT TCTACGAATC TGATGATGAT CAGAAGGAAA AGACTGACAA AAAGAAGAAG
CCGTATACTA TGGATCCAGA TCATAGACTC TTAATTAGAA ATACAAAGCC TTTGCTTCAG
AGCAGGAATG CTGCGGTGGT TATGGCAGTT GCTCAGCTGT ATTGGCACAT ATCACCAAAA
TCTGAAGCTG GCATAATTTC TAAATCACTA GTGCGTTTAC TTCGTAGCAA TAGGGAGGTG
CAGTATATTG TCCTACAAAA TATAGCAACT ATGTCAATTC AAAGAAAGGG GATGTTTGAA
CCTTATCTGA AGAGTTTCTA TGTTAGGTCA ACTGATCCAA CTATGATCAA GACACTGAAG
CTTGAAATTT TGACAAACTT GGCAAATGAA GCCAACATAT CAACTCTTCT TCGAGAATTT
CAGACCTATG TGAAAAGCCA GGATAAACAA TTTGCAGCAG CCACTATTCA GACTATAGGC
AGATGTGCAA CCAACATCTT GGAAGTCACT GACACGTGCC TCAATGGCTT GGTCTGTCTG
CTGTCCAACA GGGATGAAAT AGTTGTTGCT GAAAGTGTGG TTGTTATAAA GAAATTACTG
CAAATGCAAC CTGCACAACA TGGTGAAATT ATTAAACATA TGGCCAAACT CCTGGACAGT
ATCACTGTTC CTGTTGCTAG AGCAAGTATT CTTTGGCTAA TTGGAGAAAA CTGTGAACGA
GTTCCTAAAA TTGCCCCTGA TGTTTTGAGG AAGATGGCTA AAAGCTTCAC TAGTGAAGAT
GATCTGGTAA AACTGCAGAT ATTAAATCTG GGAGCAAAAT TGTATTTAAC CAACTCCAAA
CAGACAAAAT TGCTTACCCA GTACATATTA AATCTCGGCA AGTATGATCA AAACTACGAC
ATCAGAGACC GTACAAGATT TATTAGGCAG CTTATTGTTC CGAATGTAAA GAGTGGAGCT
TTAAGTAAAT ATGCCAAAAA AATATTCCTA GCACAAAAGC CTGCACCACT GCTTGAGTCT
CCTTTTAAAG ATAGAGATCA TTTCCAGCTT GGCACCTTAT CTCATACTCT CAACATTAAA
GCTACTGGGT ACCTGGAATT ATCTAATTGG CCAGAGGTGG CGCCCGACCC ATCAGTTCGA
AATGTAGAAG TAATAGAGTT GGCAAAAGAA TGGACCCCAG CAGGAAAAGC AAAGCAAGAG
AATTCTGCTA AGAAGTTTTA TTCTGAATCT GAGGAAGAGG AGGACTCTTC TGATAGTAGC
AGTGACAGTG AGAGTGAATC TGGAAGTGAA AGTGGAGAAC AAGGCGAAAG TGGGGAGGAA
GGAGACAGCA ATGAGGACAG CAGTGAGGAC TCCTCCAGTG AGCAGGACAG TGAGAGTGGA
CGGGAGTCAG GCCTAGAAAA CAAAAGAACA GCCAAGAGGA ACTCAAAAGC CAAAGGAAAA
AGTGATTCTG AAGATGGGGA GAAGGAAAAT GAAAAATCTA AAACTTCAGA TTCTTCAAAT
GACGAATCTA GTTCAATAGA AGACAGTTCT TCCGATTCTG AATCAGAGTC AGAACCTGAA
AGTGAATCTG AATCCAGAAG AGTCACTAAG GAGAAAGAAA AGAAAACAAA GCAAGATAGA
ACTCCTCTTA CCAAAGATGT TTCACTTCTA GATCTGGATG ATTTTAACCC AGTATCCACT
CCAGTTGCAC TTCCCACACC AGCTCTTTCT CCAAGTTTGA TGGCTGATCT TGAAGGTTTA
CACTTGTCAA CTTCCTCTTC AGTCATCAGT GTCAGTACTC CTGCATTTGT ACCAACGAAA
ACTCACGTGC TGCTTCATCG AATGAGTGGA AAAGGACTAG CTGCCCATTA TTTCTTTCCA
AGACAGCCTT GCATTTTTGG TGATAAGATG GTCTCTATAC AAATAACACT GAATAACACT
ACTGATCGAA AGATAGAAAA TATCCACATA GGGGAAAAAA AACTTCCTAT AGGCATGAAA
ATGCATGTTT TTAATCCAAT AGACTCTCTT GAGCCTGAGG GATCCATTAC AGTTTCAATG
GGTATTGACT TTTGTGATTC TACTCAGACT GCCAGTTTCC AGTTGTGTAC CAAGGATGAT
TGCTTCAATG TTAATATTCA GCCACCTGTT GGAGAACTGC TTTTACCTGT GGCCATGTCA
GAGAAAGATT TTAAGAAAGA GCAAGGAGTG CTAACAGGAA TGAATGAAAC TTCTGCTGTA
ATCATTGCTG CACCACAGAA TTTCACTCCC TCTGTGATCT TTCAGAAGGT TGTAAATGTA
GCCAATGTAG GTGCAGTCCC TTCTGGCCAG GATAATATAC ACAGGTTTGC AGCTAAAACT
GTGCACAGTG GGTCATTGAT GCTAGTCACA GTGGAACTGA AGGAAGGCTC TACAGCCCAG
CTTATCATAA ACACTGAGAA AACTGTGATT GGCTCTGTTC TGCTGCGGGA ACTGAAGCCT
GTCCTGTCTC AGGGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258698.1
CDS336..3473
Misc Feature(1)306..308
Misc Feature(2)336..1931
Misc Feature(3)order(474..479,486..488,495..500,585..590,597..599,606..611,618..620,690..695,702..704,711..716,723..725,801..806,813..815,822..827)
Misc Feature(4)534..620
Misc Feature(5)570..>941
Misc Feature(6)636..728
Misc Feature(7)750..827
Misc Feature(8)<2373..>2480
Misc Feature(9)2628..3062
Exon (1)1..316
Exon (2)317..392
Exon (3)393..467
Exon (4)468..563
Exon (5)564..724
Exon (6)725..791
Exon (7)792..974
Exon (8)975..1130
Exon (9)1131..1228
Exon (10)1229..1283
Exon (11)1284..1355
Exon (12)1356..1418
Exon (13)1419..1551
Exon (14)1552..1661
Exon (15)1662..1838
Exon (16)1839..2025
Exon (17)2026..2156
Exon (18)2157..2265
Exon (19)2266..2437
Exon (20)2438..2585
Exon (21)2586..2658
Exon (22)2659..2765
Exon (23)2766..2997
Exon (24)2998..3082
Exon (25)3083..3180
Exon (26)3181..3319
Exon (27)3320..4049
Translation

Target ORF information:

RefSeq Version NM_001271769.1
Organism Homo sapiens(Human)
Definition Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271769.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGTTAGAGA GCAACAAAGA TTCTGCTAAA CTGGATGCTA TGAAGCGGAT TGTTGGGATG 
ATTGCAAAAG GGAAAAATGC ATCTGAACTG TTTCCTGCTG TTGTGAAGAA TGTGGCCAGT
AAAAATATTG AGATCAAGAA GTTGGTATAT GTTTACCTGG TTCGATATGC TGAAGAACAG
CAGGATCTTG CACTCCTGTC CATAAGCACT TTTCAGCGAG CTCTGAAGGA CCCAAACCAA
CTAATTCGTG CAAGCGCTTT GAGAGTTCTG TCAAGTATTA GAGTGCCAAT TATTGTACCT
ATCATGATGC TTGCTATTAA GGAAGCTTCT GCTGACTTAT CACCATATGT TAGGAAGAAT
GCAGCCCATG CAATACAAAA ATTATACAGC CTTGATCCAG AGCAGAAGGA AATGTTAATT
GAAGTAATTG AAAAACTTCT GAAAGATAAA AGCACATTGG TAGCTGGCAG TGTTGTGATG
GCTTTTGAAG AAGTATGCCC GGACAGAATA GATCTGATTC ATAAAAATTA CCGCAAGCTA
TGTAACTTAC TAGTGGATGT TGAAGAGTGG GGGCAGGTTG TCATAATCCA CATGCTAACT
CGATATGCTC GGACACAGTT TGTCAGCCCT TGGAAAGAGG GTGATGAATT AGAAGACAAT
GGAAAGAATT TCTACGAATC TGATGATGAT CAGAAGGAAA AGACTGACAA AAAGAAGAAG
CCGTATACTA TGGATCCAGA TCATAGACTC TTAATTAGAA ATACAAAGCC TTTGCTTCAG
AGCAGGAATG CTGCGGTGGT TATGGCAGTT GCTCAGCTGT ATTGGCACAT ATCACCAAAA
TCTGAAGCTG GCATAATTTC TAAATCACTA GTGCGTTTAC TTCGTAGCAA TAGGGAGGTG
CAGTATATTG TCCTACAAAA TATAGCAACT ATGTCAATTC AAAGAAAGGG GATGTTTGAA
CCTTATCTGA AGAGTTTCTA TGTTAGGTCA ACTGATCCAA CTATGATCAA GACACTGAAG
CTTGAAATTT TGACAAACTT GGCAAATGAA GCCAACATAT CAACTCTTCT TCGAGAATTT
CAGACCTATG TGAAAAGCCA GGATAAACAA TTTGCAGCAG CCACTATTCA GACTATAGGC
AGATGTGCAA CCAACATCTT GGAAGTCACT GACACGTGCC TCAATGGCTT GGTCTGTCTG
CTGTCCAACA GGGATGAAAT AGTTGTTGCT GAAAGTGTGG TTGTTATAAA GAAATTACTG
CAAATGCAAC CTGCACAACA TGGTGAAATT ATTAAACATA TGGCCAAACT CCTGGACAGT
ATCACTGTTC CTGTTGCTAG AGCAAGTATT CTTTGGCTAA TTGGAGAAAA CTGTGAACGA
GTTCCTAAAA TTGCCCCTGA TGTTTTGAGG AAGATGGCTA AAAGCTTCAC TAGTGAAGAT
GATCTGGTAA AACTGCAGAT ATTAAATCTG GGAGCAAAAT TGTATTTAAC CAACTCCAAA
CAGACAAAAT TGCTTACCCA GTACATATTA AATCTCGGCA AGTATGATCA AAACTACGAC
ATCAGAGACC GTACAAGATT TATTAGGCAG CTTATTGTTC CGAATGTAAA GAGTGGAGCT
TTAAGTAAAT ATGCCAAAAA AATATTCCTA GCACAAAAGC CTGCACCACT GCTTGAGTCT
CCTTTTAAAG ATAGAGATCA TTTCCAGCTT GGCACCTTAT CTCATACTCT CAACATTAAA
GCTACTGGGT ACCTGGAATT ATCTAATTGG CCAGAGGTGG CGCCCGACCC ATCAGTTCGA
AATGTAGAAG TAATAGAGTT GGCAAAAGAA TGGACCCCAG CAGGAAAAGC AAAGCAAGAG
AATTCTGCTA AGAAGTTTTA TTCTGAATCT GAGGAAGAGG AGGACTCTTC TGATAGTAGC
AGTGACAGTG AGAGTGAATC TGGAAGTGAA AGTGGAGAAC AAGGCGAAAG TGGGGAGGAA
GGAGACAGCA ATGAGGACAG CAGTGAGGAC TCCTCCAGTG AGCAGGACAG TGAGAGTGGA
CGGGAGTCAG GCCTAGAAAA CAAAAGAACA GCCAAGAGGA ACTCAAAAGC CAAAGGAAAA
AGTGATTCTG AAGATGGGGA GAAGGAAAAT GAAAAATCTA AAACTTCAGA TTCTTCAAAT
GACGAATCTA GTTCAATAGA AGACAGTTCT TCCGATTCTG AATCAGAGTC AGAACCTGAA
AGTGAATCTG AATCCAGAAG AGTCACTAAG GAGAAAGAAA AGAAAACAAA GCAAGATAGA
ACTCCTCTTA CCAAAGATGT TTCACTTCTA GATCTGGATG ATTTTAACCC AGTATCCACT
CCAGTTGCAC TTCCCACACC AGCTCTTTCT CCAAGTTTGA TGGCTGATCT TGAAGGTTTA
CACTTGTCAA CTTCCTCTTC AGTCATCAGT GTCAGTACTC CTGCATTTGT ACCAACGAAA
ACTCACGTGC TGCTTCATCG AATGAGTGGA AAAGGACTAG CTGCCCATTA TTTCTTTCCA
AGACAGCCTT GCATTTTTGG TGATAAGATG GTCTCTATAC AAATAACACT GAATAACACT
ACTGATCGAA AGATAGAAAA TATCCACATA GGGGAAAAAA AACTTCCTAT AGGCATGAAA
ATGCATGTTT TTAATCCAAT AGACTCTCTT GAGCCTGAGG GATCCATTAC AGTTTCAATG
GGTATTGACT TTTGTGATTC TACTCAGACT GCCAGTTTCC AGTTGTGTAC CAAGGATGAT
TGCTTCAATG TTAATATTCA GCCACCTGTT GGAGAACTGC TTTTACCTGT GGCCATGTCA
GAGAAAGATT TTAAGAAAGA GCAAGGAGTG CTAACAGGAA TGAATGAAAC TTCTGCTGTA
ATCATTGCTG CACCACAGAA TTTCACTCCC TCTGTGATCT TTCAGAAGGT TGTAAATGTA
GCCAATGTAG GTGCAGTCCC TTCTGGCCAG GATAATATAC ACAGGTTTGC AGCTAAAACT
GTGCACAGTG GGTCATTGAT GCTAGTCACA GTGGAACTGA AGGAAGGCTC TACAGCCCAG
CTTATCATAA ACACTGAGAA AACTGTGATT GGCTCTGTTC TGCTGCGGGA ACTGAAGCCT
GTCCTGTCTC AGGGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39763
Clone ID Related Accession (Same CDS sequence) XM_005248618.3
Accession Version XM_005248618.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product AP-3 complex subunit beta-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGTCCAGCA ATAGTTTTCC TTACAATGAG CAGTCCGGAG GAGGGGAGGC GACGGAGCTG 
GGTCAGGAGG CGACCTCAAC CATTTCCCCC TCGGGGGCCT TCGGCCTCTT TAGCAGCGAT
TTGAAGAAGA ATGAAGATCT AAAGCAAATG TTAGAGAGCA ACAAAGATTC TGCTAAACTG
GATGCTATGA AGCGGATTGT TGGGATGATT GCAAAAGGGA AAAATGCATC TGAACTGTTT
CCTGCTGTTG TGAAGAATGT GGCCAGTAAA AATATTGAGA TCAAGAAGTT GGTATATGTT
TACCTGGTTC GATATGCTGA AGAACAGCAG GATCTTGCAC TCCTGTCCAT AAGCACTTTT
CAGCGAGCTC TGAAGGACCC AAACCAACTA ATTCGTGCAA GCGCTTTGAG AGTTCTGTCA
AGTATTAGAG TGCCAATTAT TGTACCTATC ATGATGCTTG CTATTAAGGA AGCTTCTGCT
GACTTATCAC CATATGTTAG GAAGAATGCA GCCCATGCAA TACAAAAATT ATACAGCCTT
GATCCAGAGC AGAAGGAAAT GTTAATTGAA GTAATTGAAA AACTTCTGAA AGATAAAAGC
ACATTGGTAG CTGGCAGTGT TGTGATGGCT TTTGAAGAAG TATGCCCGGA CAGAATAGAT
CTGATTCATA AAAATTACCG CAAGCTATGT AACTTACTAG TGGATGTTGA AGAGTGGGGG
CAGGTTGTCA TAATCCACAT GCTAACTCGA TATGCTCGGA CACAGTTTGT CAGCCCTTGG
AAAGAGGGTG ATGAATTAGA AGACAATGGA AAGAATTTCT ACGAATCTGA TGATGATCAG
AAGGAAAAGA CTGACAAAAA GAAGAAGCCG TATACTATGG ATCCAGATCA TAGACTCTTA
ATTAGAAATA CAAAGCCTTT GCTTCAGAGC AGGAATGCTG CGGTGGTTAT GGCAGTTGCT
CAGCTGTATT GGCACATATC ACCAAAATCT GAAGCTGGCA TAATTTCTAA ATCACTAGTG
CGTTTACTTC GTAGCAATAG GGAGGTGCAG TATATTGTCC TACAAAATAT AGCAACTATG
TCAATTCAAA GAAAGGGGAT GTTTGAACCT TATCTGAAGA GTTTCTATGT TAGGTCAACT
GATCCAACTA TGATCAAGAC ACTGAAGCTT GAAATTTTGA CAAACTTGGC AAATGAAGCC
AACATATCAA CTCTTCTTCG AGAATTTCAG ACCTATGTGA AAAGCCAGGA TAAACAATTT
GCAGCAGCCA CTATTCAGAC TATAGGCAGA TGTGCAACCA ACATCTTGGA AGTCACTGAC
ACGTGCCTCA ATGGCTTGGT CTGTCTGCTG TCCAACAGGG ATGAAATAGT TGTTGCTGAA
AGTGTGGTTG TTATAAAGAA ATTACTGCAA ATGCAACCTG CACAACATGG TGAAATTATT
AAACATATGG CCAAACTCCT GGACAGTATC ACTGTTCCTG TTGCTAGAGC AAGTATTCTT
TGGCTAATTG GAGAAAACTG TGAACGAGTT CCTAAAATTG CCCCTGATGT TTTGAGGAAG
ATGGCTAAAA GCTTCACTAG TGAAGATGAT CTGGTAAAAC TGCAGATATT AAATCTGGGA
GCAAAATTGT ATTTAACCAA CTCCAAACAG ACAAAATTGC TTACCCAGTA CATATTAAAT
CTCGGCAAGT ATGATCAAAA CTACGACATC AGAGACCGTA CAAGATTTAT TAGGCAGCTT
ATTGTTCCGA ATGTAAAGAG TGGAGCTTTA AGTAAATATG CCAAAAAAAT ATTCCTAGCA
CAAAAGCCTG CACCACTGCT TGAGTCTCCT TTTAAAGATA GAGATCATTT CCAGCTTGGC
ACCTTATCTC ATACTCTCAA CATTAAAGCT ACTGGGTACC TGGAATTATC TAATTGGCCA
GAGGTGGCGC CCGACCCATC AGTTCGAAAT GTAGAAGTAA TAGAGTTGGC AAAAGAATGG
ACCCCAGCAG GAAAAGCAAA GCAAGAGAAT TCTGCTAAGA AGTTTTATTC TGAATCTGAG
GAAGAGGAGG ACTCTTCTGA TAGTAGCAGT GACAGTGAGA GTGAATCTGG AAGTGAAAGT
GGAGAACAAG GCGAAAGTGG GGAGGAAGGA GACAGCAATG AGGACAGCAG TGAGGACTCC
TCCAGTGAGC AGGACAGTGA GAGTGGACGG GAGTCAGGCC TAGAAAACAA AAGAACAGCC
AAGAGGAACT CAAAAGCCAA AGGAAAAAGT GATTCTGAAG ATGGGGAGAA GGAAAATGAA
AAATCTAAAA CTTCAGATTC TTCAAATGAC GAATCTAGTT CAATAGAAGA CAGTTCTTCC
GATTCTGAAT CAGAGTCAGA ACCTGAAAGT GAATCTGAAT CCAGAAGAGT CACTAAGGAG
AAAGAAAAGA AAACAAAGCA AGATAGAACT CCTCTTACCA AAGATGTTTC ACTTCTAGAT
CTGGATGATT TTAACCCAGT ATCCACTCCA GTTGCACTTC CCACACCAGC TCTTTCTCCA
AGTTTGATGG CTGATCTTGA AGGTTTACAC TTGTCAACTT CCTCTTCAGT CATCAGTGTC
AGTACTCCTG CATTTGTACC AACGAAAACT CACGTGCTGC TTCATCGAAT GAGTGGAAAA
GGACTAGCTG CCCATTATTT CTTTCCAAGA CAGCCTTGCA TTTTTGGTGA TAAGATGGTC
TCTATACAAA TAACACTGAA TAACACTACT GATCGAAAGA TAGAAAATAT CCACATAGGG
GAAAAAAAAC TTCCTATAGG CATGAAAATG CATGTTTTTA ATCCAATAGA CTCTCTTGAG
CCTGAGGGAT CCATTACAGT TTCAATGGGT ATTGACTTTT GTGATTCTAC TCAGACTGCC
AGTTTCCAGT TGTGTACCAA GGATGATTGC TTCAATGTTA ATATTCAGCC ACCTGTTGGA
GAACTGCTTT TACCTGTGGC CATGTCAGAG AAAGATTTTA AGAAAGAGCA AGGAGTGCTA
ACAGGAATGA ATGAAACTTC TGCTGTAATC ATTGCTGCAC CACAGAATTT CACTCCCTCT
GTGATCTTTC AGAAGGTTGT AAATGTAGCC AATGTAGGTG CAGTCCCTTC TGGCCAGGAT
AATATACACA GATATGAGGA GTTAAATTTT GAAGCTCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005248675.1
CDS149..3310
Misc Feature(1)284..1891
Misc Feature(2)order(434..439,446..448,455..460,545..550,557..559,566..571,578..580,650..655,662..664,671..676,683..685,761..766,773..775,782..787)
Misc Feature(3)494..580
Misc Feature(4)530..>901
Misc Feature(5)596..688
Misc Feature(6)710..787
Misc Feature(7)<2333..>2440
Misc Feature(8)2588..3022
Translation

Target ORF information:

RefSeq Version XM_005248618.3
Organism Homo sapiens(Human)
Definition Homo sapiens adaptor related protein complex 3 beta 1 subunit (AP3B1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005248618.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
ATGTCCAGCA ATAGTTTTCC TTACAATGAG CAGTCCGGAG GAGGGGAGGC GACGGAGCTG 
GGTCAGGAGG CGACCTCAAC CATTTCCCCC TCGGGGGCCT TCGGCCTCTT TAGCAGCGAT
TTGAAGAAGA ATGAAGATCT AAAGCAAATG TTAGAGAGCA ACAAAGATTC TGCTAAACTG
GATGCTATGA AGCGGATTGT TGGGATGATT GCAAAAGGGA AAAATGCATC TGAACTGTTT
CCTGCTGTTG TGAAGAATGT GGCCAGTAAA AATATTGAGA TCAAGAAGTT GGTATATGTT
TACCTGGTTC GATATGCTGA AGAACAGCAG GATCTTGCAC TCCTGTCCAT AAGCACTTTT
CAGCGAGCTC TGAAGGACCC AAACCAACTA ATTCGTGCAA GCGCTTTGAG AGTTCTGTCA
AGTATTAGAG TGCCAATTAT TGTACCTATC ATGATGCTTG CTATTAAGGA AGCTTCTGCT
GACTTATCAC CATATGTTAG GAAGAATGCA GCCCATGCAA TACAAAAATT ATACAGCCTT
GATCCAGAGC AGAAGGAAAT GTTAATTGAA GTAATTGAAA AACTTCTGAA AGATAAAAGC
ACATTGGTAG CTGGCAGTGT TGTGATGGCT TTTGAAGAAG TATGCCCGGA CAGAATAGAT
CTGATTCATA AAAATTACCG CAAGCTATGT AACTTACTAG TGGATGTTGA AGAGTGGGGG
CAGGTTGTCA TAATCCACAT GCTAACTCGA TATGCTCGGA CACAGTTTGT CAGCCCTTGG
AAAGAGGGTG ATGAATTAGA AGACAATGGA AAGAATTTCT ACGAATCTGA TGATGATCAG
AAGGAAAAGA CTGACAAAAA GAAGAAGCCG TATACTATGG ATCCAGATCA TAGACTCTTA
ATTAGAAATA CAAAGCCTTT GCTTCAGAGC AGGAATGCTG CGGTGGTTAT GGCAGTTGCT
CAGCTGTATT GGCACATATC ACCAAAATCT GAAGCTGGCA TAATTTCTAA ATCACTAGTG
CGTTTACTTC GTAGCAATAG GGAGGTGCAG TATATTGTCC TACAAAATAT AGCAACTATG
TCAATTCAAA GAAAGGGGAT GTTTGAACCT TATCTGAAGA GTTTCTATGT TAGGTCAACT
GATCCAACTA TGATCAAGAC ACTGAAGCTT GAAATTTTGA CAAACTTGGC AAATGAAGCC
AACATATCAA CTCTTCTTCG AGAATTTCAG ACCTATGTGA AAAGCCAGGA TAAACAATTT
GCAGCAGCCA CTATTCAGAC TATAGGCAGA TGTGCAACCA ACATCTTGGA AGTCACTGAC
ACGTGCCTCA ATGGCTTGGT CTGTCTGCTG TCCAACAGGG ATGAAATAGT TGTTGCTGAA
AGTGTGGTTG TTATAAAGAA ATTACTGCAA ATGCAACCTG CACAACATGG TGAAATTATT
AAACATATGG CCAAACTCCT GGACAGTATC ACTGTTCCTG TTGCTAGAGC AAGTATTCTT
TGGCTAATTG GAGAAAACTG TGAACGAGTT CCTAAAATTG CCCCTGATGT TTTGAGGAAG
ATGGCTAAAA GCTTCACTAG TGAAGATGAT CTGGTAAAAC TGCAGATATT AAATCTGGGA
GCAAAATTGT ATTTAACCAA CTCCAAACAG ACAAAATTGC TTACCCAGTA CATATTAAAT
CTCGGCAAGT ATGATCAAAA CTACGACATC AGAGACCGTA CAAGATTTAT TAGGCAGCTT
ATTGTTCCGA ATGTAAAGAG TGGAGCTTTA AGTAAATATG CCAAAAAAAT ATTCCTAGCA
CAAAAGCCTG CACCACTGCT TGAGTCTCCT TTTAAAGATA GAGATCATTT CCAGCTTGGC
ACCTTATCTC ATACTCTCAA CATTAAAGCT ACTGGGTACC TGGAATTATC TAATTGGCCA
GAGGTGGCGC CCGACCCATC AGTTCGAAAT GTAGAAGTAA TAGAGTTGGC AAAAGAATGG
ACCCCAGCAG GAAAAGCAAA GCAAGAGAAT TCTGCTAAGA AGTTTTATTC TGAATCTGAG
GAAGAGGAGG ACTCTTCTGA TAGTAGCAGT GACAGTGAGA GTGAATCTGG AAGTGAAAGT
GGAGAACAAG GCGAAAGTGG GGAGGAAGGA GACAGCAATG AGGACAGCAG TGAGGACTCC
TCCAGTGAGC AGGACAGTGA GAGTGGACGG GAGTCAGGCC TAGAAAACAA AAGAACAGCC
AAGAGGAACT CAAAAGCCAA AGGAAAAAGT GATTCTGAAG ATGGGGAGAA GGAAAATGAA
AAATCTAAAA CTTCAGATTC TTCAAATGAC GAATCTAGTT CAATAGAAGA CAGTTCTTCC
GATTCTGAAT CAGAGTCAGA ACCTGAAAGT GAATCTGAAT CCAGAAGAGT CACTAAGGAG
AAAGAAAAGA AAACAAAGCA AGATAGAACT CCTCTTACCA AAGATGTTTC ACTTCTAGAT
CTGGATGATT TTAACCCAGT ATCCACTCCA GTTGCACTTC CCACACCAGC TCTTTCTCCA
AGTTTGATGG CTGATCTTGA AGGTTTACAC TTGTCAACTT CCTCTTCAGT CATCAGTGTC
AGTACTCCTG CATTTGTACC AACGAAAACT CACGTGCTGC TTCATCGAAT GAGTGGAAAA
GGACTAGCTG CCCATTATTT CTTTCCAAGA CAGCCTTGCA TTTTTGGTGA TAAGATGGTC
TCTATACAAA TAACACTGAA TAACACTACT GATCGAAAGA TAGAAAATAT CCACATAGGG
GAAAAAAAAC TTCCTATAGG CATGAAAATG CATGTTTTTA ATCCAATAGA CTCTCTTGAG
CCTGAGGGAT CCATTACAGT TTCAATGGGT ATTGACTTTT GTGATTCTAC TCAGACTGCC
AGTTTCCAGT TGTGTACCAA GGATGATTGC TTCAATGTTA ATATTCAGCC ACCTGTTGGA
GAACTGCTTT TACCTGTGGC CATGTCAGAG AAAGATTTTA AGAAAGAGCA AGGAGTGCTA
ACAGGAATGA ATGAAACTTC TGCTGTAATC ATTGCTGCAC CACAGAATTT CACTCCCTCT
GTGATCTTTC AGAAGGTTGT AAATGTAGCC AATGTAGGTG CAGTCCCTTC TGGCCAGGAT
AATATACACA GATATGAGGA GTTAAATTTT GAAGCTCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M


book

Synergistic defects of UNC13D and AP3B1 leading to adult hemophagocytic lymphohistiocytosis.
International journal of hematology102(4)488-92(2015 Oct)
Gao L,Zhu L,Huang L,Zhou J


book

Matrix proteins of Nipah and Hendra viruses interact with beta subunits of AP-3 complexes.
Journal of virology88(22)13099-110(2014 Nov)
Sun W,McCrory TS,Khaw WY,Petzing S,Myers T,Schmitt AP


book

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.
The Journal of biological chemistry287(23)19550-63(2012 Jun)
Bultema JJ,Ambrosio AL,Burek CL,Di Pietro SM


book

AP-3 and Rabip4' coordinately regulate spatial distribution of lysosomes.
PloS one7(10)e48142(2012)
Ivan V,Martinez-Sanchez E,Sima LE,Oorschot V,Klumperman J,Petrescu SM,van der Sluijs P


 
*
*
*
*