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CASK cDNA ORF clone, Homo sapiens (human)

Gene Symbol CASK
Entrez Gene ID 8573
Full Name calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Synonyms CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8
General protein information
Preferred Names
peripheral plasma membrane protein CASK
Names
peripheral plasma membrane protein CASK
hCASK
protein lin-2 homolog
trinucleotide repeat containing 8
calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase
NP_001119526.1
NP_001119527.1
NP_003679.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xp11.4

Summary This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]. lac of sum
Disorder MIM:

300172

Disorder Html: Mental retardation and microcephaly with pontine and cerebellar

mRNA and Protein(s)

mRNA Protein Name
XM_011543993 XP_011542295 peripheral plasma membrane protein CASK isoform X1
XM_005272686 XP_005272743 peripheral plasma membrane protein CASK isoform X2
XM_011543994 XP_011542296 peripheral plasma membrane protein CASK isoform X3
XM_011543995 XP_011542297 peripheral plasma membrane protein CASK isoform X4
XM_011543996 XP_011542298 peripheral plasma membrane protein CASK isoform X5
XM_006724566 XP_006724629 peripheral plasma membrane protein CASK isoform X6
XM_011543997 XP_011542299 peripheral plasma membrane protein CASK isoform X7
NM_003688 NP_003679 peripheral plasma membrane protein CASK isoform 1
NM_001126054 NP_001119526 peripheral plasma membrane protein CASK isoform 2
NM_001126055 NP_001119527 peripheral plasma membrane protein CASK isoform 3



Homo sapiens (human) CASK NP_003679.2
Pan troglodytes (chimpanzee) CASK XP_003317477.2
Macaca mulatta (Rhesus monkey) CASK XP_001095837.2
Canis lupus familiaris (dog) CASK XP_005641318.1
Bos taurus (cattle) CASK NP_001095951.1
Mus musculus (house mouse) Cask NP_001271432.1
Rattus norvegicus (Norway rat) Cask NP_071520.1
Gallus gallus (chicken) CASK XP_416769.3
Danio rerio (zebrafish) caska NP_694420.1
Drosophila melanogaster (fruit fly) CASK NP_001262811.1
Caenorhabditis elegans lin-2 NP_001024587.1
Xenopus (Silurana) tropicalis (western clawed frog) cask NP_001016204.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CASK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu62004 XM_011543993 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu54048 XM_005272686 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu62005 XM_011543994 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu62006 XM_011543995 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu62007 XM_011543996 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu54050 XM_006724566 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu62008 XM_011543997 PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu27417
NM_003688 Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$464.50-$650.30
$929.00
OHu27080
NM_001126054 Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu26584 NM_001126055 Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu62004
Clone ID Related Accession (Same CDS sequence) XM_011543993
Accession Version XM_011543993.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2781bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGAGA
GATTCCCCTT CCACTTCCAG ACAGTCCCCA GCTAATGGTC ATAGCAGCAC TAACAATTCT
GTTTCGGACT TGCCATCAAC TACCCAACCA AAAGGACGAC AGATCTATGT AAGAGCACAA
TTTGAATATG ATCCAGCCAA GGATGACCTC ATCCCCTGTA AAGAAGCTGG CATTCGATTC
AGAGTTGGTG ACATCATCCA GATTATTAGT AAGGATGATC ATAATTGGTG GCAGGGTAAA
CTGGAAAACT CCAAAAATGG AACTGCAGGT CTCATTCCTT CTCCTGAACT TCAGGAATGG
CGAGTAGCTT GCATTGCCAT GGAGAAGACC AAACAGGAGC AGCAGGCCAG CTGTACTTGG
TTTGGCAAGA AAAAGAAGCA GTACAAAGAT AAATATTTGG CAAAGCACAA TGCAGTGTTT
GATCAATTAG ATCTTGTCAC ATATGAAGAA GTAGTAAAAC TGCCAGCATT CAAGAGGAAA
ACACTAGTCT TATTAGGCGC ACATGGTGTT GGGAGAAGAC ACATAAAAAA CACTCTCATC
ACAAAGCACC CAGACCGGTT TGCGTACCCT ATTCCACATA CAACCAGACC TCCAAAGAAA
GACGAAGAAA ATGGAAAGAA TTATTACTTT GTATCTCATG ACCAAATGAT GCAAGACATC
TCTAATAACG AGTACTTGGA GTACGGCAGC CACGAGGATG CGATGTATGG GACAAAACTG
GAGACCATCC GGAAGATCCA CGAGCAGGGG CTGATTGCAA TACTGGACGT GGAGCCTCAG
GCACTGAAGG TCCTGAGAAC TGCAGAGTTT GCTCCTTTTG TTGTTTTCAT TGCTGCACCA
ACTATTACTC CAGGTTTAAA TGAGGATGAA TCTCTTCAGC GTCTGCAGAA GGAGTCTGAC
ATCTTACAGA GAACATATGC ACACTACTTC GATCTCACAA TTATCAACAA TGAAATTGAT
GAGACAATCA GACATCTGGA GGAAGCTGTT GAGCTCGTGT GCACAGCCCC ACAGTGGGTC
CCTGTCTCCT GGGTCTATTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542295.1
CDS91..2871
Misc Feature(1)112..1029(+)
Misc Feature(2)124..936(+)
Misc Feature(3)142..666(+)
Misc Feature(4)142..594(+)
Misc Feature(5)154..666(+)
Misc Feature(6)589..660(+)
Misc Feature(7)1126..1281(+)
Misc Feature(8)1303..1452(+)
Misc Feature(9)1552..1794(+)
Misc Feature(10)1588..1761(+)
Misc Feature(11)1936..2121(+)
Misc Feature(12)1942..2019(+)
Misc Feature(13)1951..2118(+)
Misc Feature(14)2302..2823(+)
Misc Feature(15)2314..2811(+)
Misc Feature(16)2326..2538(+)
Misc Feature(17)2326..2346(+)
Translation

Target ORF information:

RefSeq Version XM_011543993
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543993

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGAGA
GATTCCCCTT CCACTTCCAG ACAGTCCCCA GCTAATGGTC ATAGCAGCAC TAACAATTCT
GTTTCGGACT TGCCATCAAC TACCCAACCA AAAGGACGAC AGATCTATGT AAGAGCACAA
TTTGAATATG ATCCAGCCAA GGATGACCTC ATCCCCTGTA AAGAAGCTGG CATTCGATTC
AGAGTTGGTG ACATCATCCA GATTATTAGT AAGGATGATC ATAATTGGTG GCAGGGTAAA
CTGGAAAACT CCAAAAATGG AACTGCAGGT CTCATTCCTT CTCCTGAACT TCAGGAATGG
CGAGTAGCTT GCATTGCCAT GGAGAAGACC AAACAGGAGC AGCAGGCCAG CTGTACTTGG
TTTGGCAAGA AAAAGAAGCA GTACAAAGAT AAATATTTGG CAAAGCACAA TGCAGTGTTT
GATCAATTAG ATCTTGTCAC ATATGAAGAA GTAGTAAAAC TGCCAGCATT CAAGAGGAAA
ACACTAGTCT TATTAGGCGC ACATGGTGTT GGGAGAAGAC ACATAAAAAA CACTCTCATC
ACAAAGCACC CAGACCGGTT TGCGTACCCT ATTCCACATA CAACCAGACC TCCAAAGAAA
GACGAAGAAA ATGGAAAGAA TTATTACTTT GTATCTCATG ACCAAATGAT GCAAGACATC
TCTAATAACG AGTACTTGGA GTACGGCAGC CACGAGGATG CGATGTATGG GACAAAACTG
GAGACCATCC GGAAGATCCA CGAGCAGGGG CTGATTGCAA TACTGGACGT GGAGCCTCAG
GCACTGAAGG TCCTGAGAAC TGCAGAGTTT GCTCCTTTTG TTGTTTTCAT TGCTGCACCA
ACTATTACTC CAGGTTTAAA TGAGGATGAA TCTCTTCAGC GTCTGCAGAA GGAGTCTGAC
ATCTTACAGA GAACATATGC ACACTACTTC GATCTCACAA TTATCAACAA TGAAATTGAT
GAGACAATCA GACATCTGGA GGAAGCTGTT GAGCTCGTGT GCACAGCCCC ACAGTGGGTC
CCTGTCTCCT GGGTCTATTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54048
Clone ID Related Accession (Same CDS sequence) XM_005272686
Accession Version XM_005272686.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838130. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGAGCA
GTCTCACAGG TGCTGGACAG CCTGGAAGAG ATTCATGCGC TTACAGACTG CAGTGAAAAG
GACCTAGATT TTCTACACAG TGTTTTCCAG GATCAGCATC TTCACACACT ACTAGATCTG
TATGACAAAA TTAACACAAA GTCTTCACCA CAAATCAGGA ATCCTCCAAG CGATGCAGTA
CAGAGAGCCA AAGAGGTATT GGAAGAAATT TCATGTTACC CTGAGAATAA CGACGCAAAG
GAACTAAAGC GTATTTTAAC ACAACCTCAT TTCATGGCCT TACTTCAGAC TCACGACGTA
GTGGCACATG AAGTTTACAG TGATGAAGCA TTGAGGGTCA CACCTCCTCC CACCTCTCCC
TATTTAAACG GCGATTCTCC AGAAAGTGCT AACGGAGACA TGGATATGGA GAATGTGACC
AGAGTTCGGC TGGTACAGTT TCAAAAGAAC ACAGATGAAC CAATGGGAAT CACTTTAAAA
ATGAATGAAC TAAATCATTG TATTGTTGCA AGAATTATGC ATGGGGGCAT GATTCACAGG
CAAGGTACAC TTCATGTTGG TGATGAAATT CGAGAAATCA ATGGCATCAG TGTGGCTAAC
CAAACAGTGG AACAACTGCA AAAAATGCTT AGGGAAATGC GGGGGAGTAT TACCTTCAAG
ATTGTGCCAA GTTACCGCAC TCAGTCTTCG TCCTGTGAGA GAGATTCCCC TTCCACTTCC
AGACAGTCCC CAGCTAATGG TCATAGCAGC ACTAACAATT CTGTTTCGGA CTTGCCATCA
ACTACCCAAC CAAAAGGACG ACAGATCTAT GTAAGAGCAC AATTTGAATA TGATCCAGCC
AAGGATGACC TCATCCCCTG TAAAGAAGCT GGCATTCGAT TCAGAGTTGG TGACATCATC
CAGATTATTA GTAAGGATGA TCATAATTGG TGGCAGGGTA AACTGGAAAA CTCCAAAAAT
GGAACTGCAG GTCTCATTCC TTCTCCTGAA CTTCAGGAAT GGCGAGTAGC TTGCATTGCC
ATGGAGAAGA CCAAACAGGA GCAGCAGGCC AGCTGTACTT GGTTTGGCAA GAAAAAGAAG
CAGTACAAAG ATAAATATTT GGCAAAGCAC AATGCAGTGT TTGATCAATT AGATCTTGTC
ACATATGAAG AAGTAGTAAA ACTGCCAGCA TTCAAGAGGA AAACACTAGT CTTATTAGGC
GCACATGGTG TTGGGAGAAG ACACATAAAA AACACTCTCA TCACAAAGCA CCCAGACCGG
TTTGCGTACC CTATTCCACA TACAACCAGA CCTCCAAAGA AAGACGAAGA AAATGGAAAG
AATTATTACT TTGTATCTCA TGACCAAATG ATGCAAGACA TCTCTAATAA CGAGTACTTG
GAGTACGGCA GCCACGAGGA TGCGATGTAT GGGACAAAAC TGGAGACCAT CCGGAAGATC
CACGAGCAGG GGCTGATTGC AATACTGGAC GTGGAGCCTC AGGCACTGAA GGTCCTGAGA
ACTGCAGAGT TTGCTCCTTT TGTTGTTTTC ATTGCTGCAC CAACTATTAC TCCAGGTTTA
AATGAGGATG AATCTCTTCA GCGTCTGCAG AAGGAGTCTG ACATCTTACA GAGAACATAT
GCACACTACT TCGATCTCAC AATTATCAAC AATGAAATTG ATGAGACAAT CAGACATCTG
GAGGAAGCTG TTGAGCTCGT GTGCACAGCC CCACAGTGGG TCCCTGTCTC CTGGGTCTAT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005272743.1
CDS91..2853
Misc Feature(1)112..1011(+)
Misc Feature(2)124..918(+)
Misc Feature(3)142..648(+)
Misc Feature(4)142..576(+)
Misc Feature(5)154..648(+)
Misc Feature(6)571..642(+)
Misc Feature(7)1108..1263(+)
Misc Feature(8)1285..1434(+)
Misc Feature(9)1534..1776(+)
Misc Feature(10)1570..1743(+)
Misc Feature(11)1918..2103(+)
Misc Feature(12)1924..2001(+)
Misc Feature(13)1933..2100(+)
Misc Feature(14)2284..2805(+)
Misc Feature(15)2296..2793(+)
Misc Feature(16)2308..2520(+)
Misc Feature(17)2308..2328(+)
Translation

Target ORF information:

RefSeq Version XM_005272686
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005272686

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGAGCA
GTCTCACAGG TGCTGGACAG CCTGGAAGAG ATTCATGCGC TTACAGACTG CAGTGAAAAG
GACCTAGATT TTCTACACAG TGTTTTCCAG GATCAGCATC TTCACACACT ACTAGATCTG
TATGACAAAA TTAACACAAA GTCTTCACCA CAAATCAGGA ATCCTCCAAG CGATGCAGTA
CAGAGAGCCA AAGAGGTATT GGAAGAAATT TCATGTTACC CTGAGAATAA CGACGCAAAG
GAACTAAAGC GTATTTTAAC ACAACCTCAT TTCATGGCCT TACTTCAGAC TCACGACGTA
GTGGCACATG AAGTTTACAG TGATGAAGCA TTGAGGGTCA CACCTCCTCC CACCTCTCCC
TATTTAAACG GCGATTCTCC AGAAAGTGCT AACGGAGACA TGGATATGGA GAATGTGACC
AGAGTTCGGC TGGTACAGTT TCAAAAGAAC ACAGATGAAC CAATGGGAAT CACTTTAAAA
ATGAATGAAC TAAATCATTG TATTGTTGCA AGAATTATGC ATGGGGGCAT GATTCACAGG
CAAGGTACAC TTCATGTTGG TGATGAAATT CGAGAAATCA ATGGCATCAG TGTGGCTAAC
CAAACAGTGG AACAACTGCA AAAAATGCTT AGGGAAATGC GGGGGAGTAT TACCTTCAAG
ATTGTGCCAA GTTACCGCAC TCAGTCTTCG TCCTGTGAGA GAGATTCCCC TTCCACTTCC
AGACAGTCCC CAGCTAATGG TCATAGCAGC ACTAACAATT CTGTTTCGGA CTTGCCATCA
ACTACCCAAC CAAAAGGACG ACAGATCTAT GTAAGAGCAC AATTTGAATA TGATCCAGCC
AAGGATGACC TCATCCCCTG TAAAGAAGCT GGCATTCGAT TCAGAGTTGG TGACATCATC
CAGATTATTA GTAAGGATGA TCATAATTGG TGGCAGGGTA AACTGGAAAA CTCCAAAAAT
GGAACTGCAG GTCTCATTCC TTCTCCTGAA CTTCAGGAAT GGCGAGTAGC TTGCATTGCC
ATGGAGAAGA CCAAACAGGA GCAGCAGGCC AGCTGTACTT GGTTTGGCAA GAAAAAGAAG
CAGTACAAAG ATAAATATTT GGCAAAGCAC AATGCAGTGT TTGATCAATT AGATCTTGTC
ACATATGAAG AAGTAGTAAA ACTGCCAGCA TTCAAGAGGA AAACACTAGT CTTATTAGGC
GCACATGGTG TTGGGAGAAG ACACATAAAA AACACTCTCA TCACAAAGCA CCCAGACCGG
TTTGCGTACC CTATTCCACA TACAACCAGA CCTCCAAAGA AAGACGAAGA AAATGGAAAG
AATTATTACT TTGTATCTCA TGACCAAATG ATGCAAGACA TCTCTAATAA CGAGTACTTG
GAGTACGGCA GCCACGAGGA TGCGATGTAT GGGACAAAAC TGGAGACCAT CCGGAAGATC
CACGAGCAGG GGCTGATTGC AATACTGGAC GTGGAGCCTC AGGCACTGAA GGTCCTGAGA
ACTGCAGAGT TTGCTCCTTT TGTTGTTTTC ATTGCTGCAC CAACTATTAC TCCAGGTTTA
AATGAGGATG AATCTCTTCA GCGTCTGCAG AAGGAGTCTG ACATCTTACA GAGAACATAT
GCACACTACT TCGATCTCAC AATTATCAAC AATGAAATTG ATGAGACAAT CAGACATCTG
GAGGAAGCTG TTGAGCTCGT GTGCACAGCC CCACAGTGGG TCCCTGTCTC CTGGGTCTAT
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62005
Clone ID Related Accession (Same CDS sequence) XM_011543994
Accession Version XM_011543994.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGAGA
GATTCCCCTT CCACTTCCAG ACAGTCCCCA GCTAATGGTC ATAGCAGCAC TAACAATTCT
GTTTCGATCT ATGTAAGAGC ACAATTTGAA TATGATCCAG CCAAGGATGA CCTCATCCCC
TGTAAAGAAG CTGGCATTCG ATTCAGAGTT GGTGACATCA TCCAGATTAT TAGTAAGGAT
GATCATAATT GGTGGCAGGG TAAACTGGAA AACTCCAAAA ATGGAACTGC AGGTCTCATT
CCTTCTCCTG AACTTCAGGA ATGGCGAGTA GCTTGCATTG CCATGGAGAA GACCAAACAG
GAGCAGCAGG CCAGCTGTAC TTGGTTTGGC AAGAAAAAGA AGCAGTACAA AGATAAATAT
TTGGCAAAGC ACAATGCAGT GTTTGATCAA TTAGATCTTG TCACATATGA AGAAGTAGTA
AAACTGCCAG CATTCAAGAG GAAAACACTA GTCTTATTAG GCGCACATGG TGTTGGGAGA
AGACACATAA AAAACACTCT CATCACAAAG CACCCAGACC GGTTTGCGTA CCCTATTCCA
CATACAACCA GACCTCCAAA GAAAGACGAA GAAAATGGAA AGAATTATTA CTTTGTATCT
CATGACCAAA TGATGCAAGA CATCTCTAAT AACGAGTACT TGGAGTACGG CAGCCACGAG
GATGCGATGT ATGGGACAAA ACTGGAGACC ATCCGGAAGA TCCACGAGCA GGGGCTGATT
GCAATACTGG ACGTGGAGCC TCAGGCACTG AAGGTCCTGA GAACTGCAGA GTTTGCTCCT
TTTGTTGTTT TCATTGCTGC ACCAACTATT ACTCCAGGTT TAAATGAGGA TGAATCTCTT
CAGCGTCTGC AGAAGGAGTC TGACATCTTA CAGAGAACAT ATGCACACTA CTTCGATCTC
ACAATTATCA ACAATGAAAT TGATGAGACA ATCAGACATC TGGAGGAAGC TGTTGAGCTC
GTGTGCACAG CCCCACAGTG GGTCCCTGTC TCCTGGGTCT ATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542296.1
CDS91..2835
Misc Feature(1)112..1029(+)
Misc Feature(2)124..936(+)
Misc Feature(3)142..666(+)
Misc Feature(4)142..594(+)
Misc Feature(5)154..666(+)
Misc Feature(6)589..660(+)
Misc Feature(7)1126..1281(+)
Misc Feature(8)1303..1452(+)
Misc Feature(9)1552..1794(+)
Misc Feature(10)1588..1761(+)
Misc Feature(11)1900..2085(+)
Misc Feature(12)1906..1983(+)
Misc Feature(13)1915..2082(+)
Misc Feature(14)2266..2787(+)
Misc Feature(15)2278..2775(+)
Misc Feature(16)2290..2502(+)
Misc Feature(17)2290..2310(+)
Translation

Target ORF information:

RefSeq Version XM_011543994
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543994

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGAGA
GATTCCCCTT CCACTTCCAG ACAGTCCCCA GCTAATGGTC ATAGCAGCAC TAACAATTCT
GTTTCGATCT ATGTAAGAGC ACAATTTGAA TATGATCCAG CCAAGGATGA CCTCATCCCC
TGTAAAGAAG CTGGCATTCG ATTCAGAGTT GGTGACATCA TCCAGATTAT TAGTAAGGAT
GATCATAATT GGTGGCAGGG TAAACTGGAA AACTCCAAAA ATGGAACTGC AGGTCTCATT
CCTTCTCCTG AACTTCAGGA ATGGCGAGTA GCTTGCATTG CCATGGAGAA GACCAAACAG
GAGCAGCAGG CCAGCTGTAC TTGGTTTGGC AAGAAAAAGA AGCAGTACAA AGATAAATAT
TTGGCAAAGC ACAATGCAGT GTTTGATCAA TTAGATCTTG TCACATATGA AGAAGTAGTA
AAACTGCCAG CATTCAAGAG GAAAACACTA GTCTTATTAG GCGCACATGG TGTTGGGAGA
AGACACATAA AAAACACTCT CATCACAAAG CACCCAGACC GGTTTGCGTA CCCTATTCCA
CATACAACCA GACCTCCAAA GAAAGACGAA GAAAATGGAA AGAATTATTA CTTTGTATCT
CATGACCAAA TGATGCAAGA CATCTCTAAT AACGAGTACT TGGAGTACGG CAGCCACGAG
GATGCGATGT ATGGGACAAA ACTGGAGACC ATCCGGAAGA TCCACGAGCA GGGGCTGATT
GCAATACTGG ACGTGGAGCC TCAGGCACTG AAGGTCCTGA GAACTGCAGA GTTTGCTCCT
TTTGTTGTTT TCATTGCTGC ACCAACTATT ACTCCAGGTT TAAATGAGGA TGAATCTCTT
CAGCGTCTGC AGAAGGAGTC TGACATCTTA CAGAGAACAT ATGCACACTA CTTCGATCTC
ACAATTATCA ACAATGAAAT TGATGAGACA ATCAGACATC TGGAGGAAGC TGTTGAGCTC
GTGTGCACAG CCCCACAGTG GGTCCCTGTC TCCTGGGTCT ATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62006
Clone ID Related Accession (Same CDS sequence) XM_011543995
Accession Version XM_011543995.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2712bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGGAC
TTGCCATCAA CTACCCAACC AAAAGGACGA CAGATCTATG TAAGAGCACA ATTTGAATAT
GATCCAGCCA AGGATGACCT CATCCCCTGT AAAGAAGCTG GCATTCGATT CAGAGTTGGT
GACATCATCC AGATTATTAG TAAGGATGAT CATAATTGGT GGCAGGGTAA ACTGGAAAAC
TCCAAAAATG GAACTGCAGG TCTCATTCCT TCTCCTGAAC TTCAGGAATG GCGAGTAGCT
TGCATTGCCA TGGAGAAGAC CAAACAGGAG CAGCAGGCCA GCTGTACTTG GTTTGGCAAG
AAAAAGAAGC AGTACAAAGA TAAATATTTG GCAAAGCACA ATGCAGTGTT TGATCAATTA
GATCTTGTCA CATATGAAGA AGTAGTAAAA CTGCCAGCAT TCAAGAGGAA AACACTAGTC
TTATTAGGCG CACATGGTGT TGGGAGAAGA CACATAAAAA ACACTCTCAT CACAAAGCAC
CCAGACCGGT TTGCGTACCC TATTCCACAT ACAACCAGAC CTCCAAAGAA AGACGAAGAA
AATGGAAAGA ATTATTACTT TGTATCTCAT GACCAAATGA TGCAAGACAT CTCTAATAAC
GAGTACTTGG AGTACGGCAG CCACGAGGAT GCGATGTATG GGACAAAACT GGAGACCATC
CGGAAGATCC ACGAGCAGGG GCTGATTGCA ATACTGGACG TGGAGCCTCA GGCACTGAAG
GTCCTGAGAA CTGCAGAGTT TGCTCCTTTT GTTGTTTTCA TTGCTGCACC AACTATTACT
CCAGGTTTAA ATGAGGATGA ATCTCTTCAG CGTCTGCAGA AGGAGTCTGA CATCTTACAG
AGAACATATG CACACTACTT CGATCTCACA ATTATCAACA ATGAAATTGA TGAGACAATC
AGACATCTGG AGGAAGCTGT TGAGCTCGTG TGCACAGCCC CACAGTGGGT CCCTGTCTCC
TGGGTCTATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542297.1
CDS91..2802
Misc Feature(1)112..1029(+)
Misc Feature(2)124..936(+)
Misc Feature(3)142..666(+)
Misc Feature(4)142..594(+)
Misc Feature(5)154..666(+)
Misc Feature(6)589..660(+)
Misc Feature(7)1126..1281(+)
Misc Feature(8)1303..1452(+)
Misc Feature(9)1552..1794(+)
Misc Feature(10)1588..1761(+)
Misc Feature(11)1867..2052(+)
Misc Feature(12)1873..1950(+)
Misc Feature(13)1882..2049(+)
Misc Feature(14)2233..2754(+)
Misc Feature(15)2245..2742(+)
Misc Feature(16)2257..2469(+)
Misc Feature(17)2257..2277(+)
Translation

Target ORF information:

RefSeq Version XM_011543995
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543995

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGGAC
TTGCCATCAA CTACCCAACC AAAAGGACGA CAGATCTATG TAAGAGCACA ATTTGAATAT
GATCCAGCCA AGGATGACCT CATCCCCTGT AAAGAAGCTG GCATTCGATT CAGAGTTGGT
GACATCATCC AGATTATTAG TAAGGATGAT CATAATTGGT GGCAGGGTAA ACTGGAAAAC
TCCAAAAATG GAACTGCAGG TCTCATTCCT TCTCCTGAAC TTCAGGAATG GCGAGTAGCT
TGCATTGCCA TGGAGAAGAC CAAACAGGAG CAGCAGGCCA GCTGTACTTG GTTTGGCAAG
AAAAAGAAGC AGTACAAAGA TAAATATTTG GCAAAGCACA ATGCAGTGTT TGATCAATTA
GATCTTGTCA CATATGAAGA AGTAGTAAAA CTGCCAGCAT TCAAGAGGAA AACACTAGTC
TTATTAGGCG CACATGGTGT TGGGAGAAGA CACATAAAAA ACACTCTCAT CACAAAGCAC
CCAGACCGGT TTGCGTACCC TATTCCACAT ACAACCAGAC CTCCAAAGAA AGACGAAGAA
AATGGAAAGA ATTATTACTT TGTATCTCAT GACCAAATGA TGCAAGACAT CTCTAATAAC
GAGTACTTGG AGTACGGCAG CCACGAGGAT GCGATGTATG GGACAAAACT GGAGACCATC
CGGAAGATCC ACGAGCAGGG GCTGATTGCA ATACTGGACG TGGAGCCTCA GGCACTGAAG
GTCCTGAGAA CTGCAGAGTT TGCTCCTTTT GTTGTTTTCA TTGCTGCACC AACTATTACT
CCAGGTTTAA ATGAGGATGA ATCTCTTCAG CGTCTGCAGA AGGAGTCTGA CATCTTACAG
AGAACATATG CACACTACTT CGATCTCACA ATTATCAACA ATGAAATTGA TGAGACAATC
AGACATCTGG AGGAAGCTGT TGAGCTCGTG TGCACAGCCC CACAGTGGGT CCCTGTCTCC
TGGGTCTATT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62007
Clone ID Related Accession (Same CDS sequence) XM_011543996
Accession Version XM_011543996.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGATC
TATGTAAGAG CACAATTTGA ATATGATCCA GCCAAGGATG ACCTCATCCC CTGTAAAGAA
GCTGGCATTC GATTCAGAGT TGGTGACATC ATCCAGATTA TTAGTAAGGA TGATCATAAT
TGGTGGCAGG GTAAACTGGA AAACTCCAAA AATGGAACTG CAGGTCTCAT TCCTTCTCCT
GAACTTCAGG AATGGCGAGT AGCTTGCATT GCCATGGAGA AGACCAAACA GGAGCAGCAG
GCCAGCTGTA CTTGGTTTGG CAAGAAAAAG AAGCAGTACA AAGATAAATA TTTGGCAAAG
CACAATGCAG TGTTTGATCA ATTAGATCTT GTCACATATG AAGAAGTAGT AAAACTGCCA
GCATTCAAGA GGAAAACACT AGTCTTATTA GGCGCACATG GTGTTGGGAG AAGACACATA
AAAAACACTC TCATCACAAA GCACCCAGAC CGGTTTGCGT ACCCTATTCC ACATACAACC
AGACCTCCAA AGAAAGACGA AGAAAATGGA AAGAATTATT ACTTTGTATC TCATGACCAA
ATGATGCAAG ACATCTCTAA TAACGAGTAC TTGGAGTACG GCAGCCACGA GGATGCGATG
TATGGGACAA AACTGGAGAC CATCCGGAAG ATCCACGAGC AGGGGCTGAT TGCAATACTG
GACGTGGAGC CTCAGGCACT GAAGGTCCTG AGAACTGCAG AGTTTGCTCC TTTTGTTGTT
TTCATTGCTG CACCAACTAT TACTCCAGGT TTAAATGAGG ATGAATCTCT TCAGCGTCTG
CAGAAGGAGT CTGACATCTT ACAGAGAACA TATGCACACT ACTTCGATCT CACAATTATC
AACAATGAAA TTGATGAGAC AATCAGACAT CTGGAGGAAG CTGTTGAGCT CGTGTGCACA
GCCCCACAGT GGGTCCCTGT CTCCTGGGTC TATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542298.1
CDS91..2766
Misc Feature(1)112..1029(+)
Misc Feature(2)124..936(+)
Misc Feature(3)142..666(+)
Misc Feature(4)142..594(+)
Misc Feature(5)154..666(+)
Misc Feature(6)589..660(+)
Misc Feature(7)1126..1281(+)
Misc Feature(8)1303..1452(+)
Misc Feature(9)1552..1794(+)
Misc Feature(10)1588..1761(+)
Misc Feature(11)1831..2016(+)
Misc Feature(12)1837..1914(+)
Misc Feature(13)1846..2013(+)
Misc Feature(14)2197..2718(+)
Misc Feature(15)2209..2706(+)
Misc Feature(16)2221..2433(+)
Misc Feature(17)2221..2241(+)
Translation

Target ORF information:

RefSeq Version XM_011543996
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543996

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGGTAAGAGA
TGGATTTCAA ATCTAAAGCG GGAAGCCAGT ATCTGTCATA TGCTGAAACA TCCACACATT
GTAGAGTTAT TGGAGACATA TAGCTCAGAT GGAATGCTTT ACATGGTTTT CGAATTTATG
GATGGAGCAG ATCTGTGTTT TGAAATCGTA AAGCGAGCTG ACGCTGGTTT TGTGTACAGT
GAAGCTGTAG CCAGCCATTA TATGAGACAG ATACTGGAAG CTCTACGCTA CTGCCATGAT
AATAACATAA TTCACAGGGA TGTGAAGCCC CACTGTGTTC TCCTTGCCTC AAAAGAAAAC
TCGGCACCTG TTAAACTTGG AGGCTTTGGG GTAGCTATTC AATTAGGGGA GTCTGGACTT
GTAGCTGGAG GACGTGTTGG AACACCTCAT TTTATGGCAC CAGAAGTGGT CAAAAGAGAG
CCTTACGGAA AGCCTGTAGA CGTCTGGGGG TGCGGTGTGA TCCTTTTTAT CCTGCTCAGT
GGTTGTTTGC CTTTTTACGG AACCAAGGAA AGATTGTTTG AAGGCATTAT TAAAGGAAAA
TATAAGATGA ATCCAAGGCA GTGGAGCCAT ATCTCTGAAA GTGCCAAAGA CCTAGTACGT
CGCATGCTGA TGCTGGATCC AGCTGAAAGG ATCACTGTTT ATGAAGCACT GAATCACCCA
TGGCTTAAGG AGCGGGATCG TTACGCCTAC AAGATTCATC TTCCAGAAAC AGTAGAGCAG
CTGAGGAAAT TCAATGCAAG GAGGAAACTA AAGGGTGCAG TACTAGCCGC TGTGTCAAGT
CACAAATTCA ACTCATTCTA TGGGGATCCC CCTGAAGAGT TACCAGATTT CTCCGAAGAC
CCTACCTCCT CAGGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGATC
TATGTAAGAG CACAATTTGA ATATGATCCA GCCAAGGATG ACCTCATCCC CTGTAAAGAA
GCTGGCATTC GATTCAGAGT TGGTGACATC ATCCAGATTA TTAGTAAGGA TGATCATAAT
TGGTGGCAGG GTAAACTGGA AAACTCCAAA AATGGAACTG CAGGTCTCAT TCCTTCTCCT
GAACTTCAGG AATGGCGAGT AGCTTGCATT GCCATGGAGA AGACCAAACA GGAGCAGCAG
GCCAGCTGTA CTTGGTTTGG CAAGAAAAAG AAGCAGTACA AAGATAAATA TTTGGCAAAG
CACAATGCAG TGTTTGATCA ATTAGATCTT GTCACATATG AAGAAGTAGT AAAACTGCCA
GCATTCAAGA GGAAAACACT AGTCTTATTA GGCGCACATG GTGTTGGGAG AAGACACATA
AAAAACACTC TCATCACAAA GCACCCAGAC CGGTTTGCGT ACCCTATTCC ACATACAACC
AGACCTCCAA AGAAAGACGA AGAAAATGGA AAGAATTATT ACTTTGTATC TCATGACCAA
ATGATGCAAG ACATCTCTAA TAACGAGTAC TTGGAGTACG GCAGCCACGA GGATGCGATG
TATGGGACAA AACTGGAGAC CATCCGGAAG ATCCACGAGC AGGGGCTGAT TGCAATACTG
GACGTGGAGC CTCAGGCACT GAAGGTCCTG AGAACTGCAG AGTTTGCTCC TTTTGTTGTT
TTCATTGCTG CACCAACTAT TACTCCAGGT TTAAATGAGG ATGAATCTCT TCAGCGTCTG
CAGAAGGAGT CTGACATCTT ACAGAGAACA TATGCACACT ACTTCGATCT CACAATTATC
AACAATGAAA TTGATGAGAC AATCAGACAT CTGGAGGAAG CTGTTGAGCT CGTGTGCACA
GCCCCACAGT GGGTCCCTGT CTCCTGGGTC TATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54050
Clone ID Related Accession (Same CDS sequence) XM_006724566
Accession Version XM_006724566.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2658bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838133. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGAGCA
GTCTCACAGG TGCTGGACAG CCTGGAAGAG ATTCATGCGC TTACAGACTG CAGTGAAAAG
GACCTAGATT TTCTACACAG TGTTTTCCAG GATCAGCATC TTCACACACT ACTAGATCTG
TATGACAAAA TTAACACAAA GTCTTCACCA CAAATCAGGA ATCCTCCAAG CGATGCAGTA
CAGAGAGCCA AAGAGGTATT GGAAGAAATT TCATGTTACC CTGAGAATAA CGACGCAAAG
GAACTAAAGC GTATTTTAAC ACAACCTCAT TTCATGGCCT TACTTCAGAC TCACGACGTA
GTGGCACATG AAGTTTACAG TGATGAAGCA TTGAGGGTCA CACCTCCTCC CACCTCTCCC
TATTTAAACG GCGATTCTCC AGAAAGTGCT AACGGAGACA TGGATATGGA GAATGTGACC
AGAGTTCGGC TGGTACAGTT TCAAAAGAAC ACAGATGAAC CAATGGGAAT CACTTTAAAA
ATGAATGAAC TAAATCATTG TATTGTTGCA AGAATTATGC ATGGGGGCAT GATTCACAGG
CAAGGTACAC TTCATGTTGG TGATGAAATT CGAGAAATCA ATGGCATCAG TGTGGCTAAC
CAAACAGTGG AACAACTGCA AAAAATGCTT AGGGAAATGC GGGGGAGTAT TACCTTCAAG
ATTGTGCCAA GTTACCGCAC TCAGTCTTCG TCCTGTGAGA TCTATGTAAG AGCACAATTT
GAATATGATC CAGCCAAGGA TGACCTCATC CCCTGTAAAG AAGCTGGCAT TCGATTCAGA
GTTGGTGACA TCATCCAGAT TATTAGTAAG GATGATCATA ATTGGTGGCA GGGTAAACTG
GAAAACTCCA AAAATGGAAC TGCAGGTCTC ATTCCTTCTC CTGAACTTCA GGAATGGCGA
GTAGCTTGCA TTGCCATGGA GAAGACCAAA CAGGAGCAGC AGGCCAGCTG TACTTGGTTT
GGCAAGAAAA AGAAGCAGTA CAAAGATAAA TATTTGGCAA AGCACAATGC AGTGTTTGAT
CAATTAGATC TTGTCACATA TGAAGAAGTA GTAAAACTGC CAGCATTCAA GAGGAAAACA
CTAGTCTTAT TAGGCGCACA TGGTGTTGGG AGAAGACACA TAAAAAACAC TCTCATCACA
AAGCACCCAG ACCGGTTTGC GTACCCTATT CCACATACAA CCAGACCTCC AAAGAAAGAC
GAAGAAAATG GAAAGAATTA TTACTTTGTA TCTCATGACC AAATGATGCA AGACATCTCT
AATAACGAGT ACTTGGAGTA CGGCAGCCAC GAGGATGCGA TGTATGGGAC AAAACTGGAG
ACCATCCGGA AGATCCACGA GCAGGGGCTG ATTGCAATAC TGGACGTGGA GCCTCAGGCA
CTGAAGGTCC TGAGAACTGC AGAGTTTGCT CCTTTTGTTG TTTTCATTGC TGCACCAACT
ATTACTCCAG GTTTAAATGA GGATGAATCT CTTCAGCGTC TGCAGAAGGA GTCTGACATC
TTACAGAGAA CATATGCACA CTACTTCGAT CTCACAATTA TCAACAATGA AATTGATGAG
ACAATCAGAC ATCTGGAGGA AGCTGTTGAG CTCGTGTGCA CAGCCCCACA GTGGGTCCCT
GTCTCCTGGG TCTATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724629.1
CDS91..2748
Misc Feature(1)112..1011(+)
Misc Feature(2)124..918(+)
Misc Feature(3)142..648(+)
Misc Feature(4)142..576(+)
Misc Feature(5)154..648(+)
Misc Feature(6)571..642(+)
Misc Feature(7)1108..1263(+)
Misc Feature(8)1285..1434(+)
Misc Feature(9)1534..1776(+)
Misc Feature(10)1570..1743(+)
Misc Feature(11)1813..1998(+)
Misc Feature(12)1819..1896(+)
Misc Feature(13)1828..1995(+)
Misc Feature(14)2179..2700(+)
Misc Feature(15)2191..2688(+)
Misc Feature(16)2203..2415(+)
Misc Feature(17)2203..2223(+)
Translation

Target ORF information:

RefSeq Version XM_006724566
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724566

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGAGCA
GTCTCACAGG TGCTGGACAG CCTGGAAGAG ATTCATGCGC TTACAGACTG CAGTGAAAAG
GACCTAGATT TTCTACACAG TGTTTTCCAG GATCAGCATC TTCACACACT ACTAGATCTG
TATGACAAAA TTAACACAAA GTCTTCACCA CAAATCAGGA ATCCTCCAAG CGATGCAGTA
CAGAGAGCCA AAGAGGTATT GGAAGAAATT TCATGTTACC CTGAGAATAA CGACGCAAAG
GAACTAAAGC GTATTTTAAC ACAACCTCAT TTCATGGCCT TACTTCAGAC TCACGACGTA
GTGGCACATG AAGTTTACAG TGATGAAGCA TTGAGGGTCA CACCTCCTCC CACCTCTCCC
TATTTAAACG GCGATTCTCC AGAAAGTGCT AACGGAGACA TGGATATGGA GAATGTGACC
AGAGTTCGGC TGGTACAGTT TCAAAAGAAC ACAGATGAAC CAATGGGAAT CACTTTAAAA
ATGAATGAAC TAAATCATTG TATTGTTGCA AGAATTATGC ATGGGGGCAT GATTCACAGG
CAAGGTACAC TTCATGTTGG TGATGAAATT CGAGAAATCA ATGGCATCAG TGTGGCTAAC
CAAACAGTGG AACAACTGCA AAAAATGCTT AGGGAAATGC GGGGGAGTAT TACCTTCAAG
ATTGTGCCAA GTTACCGCAC TCAGTCTTCG TCCTGTGAGA TCTATGTAAG AGCACAATTT
GAATATGATC CAGCCAAGGA TGACCTCATC CCCTGTAAAG AAGCTGGCAT TCGATTCAGA
GTTGGTGACA TCATCCAGAT TATTAGTAAG GATGATCATA ATTGGTGGCA GGGTAAACTG
GAAAACTCCA AAAATGGAAC TGCAGGTCTC ATTCCTTCTC CTGAACTTCA GGAATGGCGA
GTAGCTTGCA TTGCCATGGA GAAGACCAAA CAGGAGCAGC AGGCCAGCTG TACTTGGTTT
GGCAAGAAAA AGAAGCAGTA CAAAGATAAA TATTTGGCAA AGCACAATGC AGTGTTTGAT
CAATTAGATC TTGTCACATA TGAAGAAGTA GTAAAACTGC CAGCATTCAA GAGGAAAACA
CTAGTCTTAT TAGGCGCACA TGGTGTTGGG AGAAGACACA TAAAAAACAC TCTCATCACA
AAGCACCCAG ACCGGTTTGC GTACCCTATT CCACATACAA CCAGACCTCC AAAGAAAGAC
GAAGAAAATG GAAAGAATTA TTACTTTGTA TCTCATGACC AAATGATGCA AGACATCTCT
AATAACGAGT ACTTGGAGTA CGGCAGCCAC GAGGATGCGA TGTATGGGAC AAAACTGGAG
ACCATCCGGA AGATCCACGA GCAGGGGCTG ATTGCAATAC TGGACGTGGA GCCTCAGGCA
CTGAAGGTCC TGAGAACTGC AGAGTTTGCT CCTTTTGTTG TTTTCATTGC TGCACCAACT
ATTACTCCAG GTTTAAATGA GGATGAATCT CTTCAGCGTC TGCAGAAGGA GTCTGACATC
TTACAGAGAA CATATGCACA CTACTTCGAT CTCACAATTA TCAACAATGA AATTGATGAG
ACAATCAGAC ATCTGGAGGA AGCTGTTGAG CTCGTGTGCA CAGCCCCACA GTGGGTCCCT
GTCTCCTGGG TCTATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62008
Clone ID Related Accession (Same CDS sequence) XM_011543997
Accession Version XM_011543997.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_079573.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCACCAG AAGTGGTCAA AAGAGAGCCT TACGGAAAGC CTGTAGACGT CTGGGGGTGC 
GGTGTGATCC TTTTTATCCT GCTCAGTGGT TGTTTGCCTT TTTACGGAAC CAAGGAAAGA
TTGTTTGAAG GCATTATTAA AGGAAAATAT AAGATGAATC CAAGGCAGTG GAGCCATATC
TCTGAAAGTG CCAAAGACCT AGTACGTCGC ATGCTGATGC TGGATCCAGC TGAAAGGATC
ACTGTTTATG AAGCACTGAA TCACCCATGG CTTAAGGAGC GGGATCGTTA CGCCTACAAG
ATTCATCTTC CAGAAACAGT AGAGCAGCTG AGGAAATTCA ATGCAAGGAG GAAACTAAAG
GGTGCAGTAC TAGCCGCTGT GTCAAGTCAC AAATTCAACT CATTCTATGG GGATCCCCCT
GAAGAGTTAC CAGATTTCTC CGAAGACCCT ACCTCCTCAG GAGCAGTCTC ACAGGTGCTG
GACAGCCTGG AAGAGATTCA TGCGCTTACA GACTGCAGTG AAAAGGACCT AGATTTTCTA
CACAGTGTTT TCCAGGATCA GCATCTTCAC ACACTACTAG ATCTGTATGA CAAAATTAAC
ACAAAGTCTT CACCACAAAT CAGGAATCCT CCAAGCGATG CAGTACAGAG AGCCAAAGAG
GTATTGGAAG AAATTTCATG TTACCCTGAG AATAACGACG CAAAGGAACT AAAGCGTATT
TTAACACAAC CTCATTTCAT GGCCTTACTT CAGACTCACG ACGTAGTGGC ACATGAAGTT
TACAGTGATG AAGCATTGAG GGTCACACCT CCTCCCACCT CTCCCTATTT AAACGGCGAT
TCTCCAGAAA GTGCTAACGG AGACATGGAT ATGGAGAATG TGACCAGAGT TCGGCTGGTA
CAGTTTCAAA AGAACACAGA TGAACCAATG GGAATCACTT TAAAAATGAA TGAACTAAAT
CATTGTATTG TTGCAAGAAT TATGCATGGG GGCATGATTC ACAGGCAAGG TACACTTCAT
GTTGGTGATG AAATTCGAGA AATCAATGGC ATCAGTGTGG CTAACCAAAC AGTGGAACAA
CTGCAAAAAA TGCTTAGGGA AATGCGGGGG AGTATTACCT TCAAGATTGT GCCAAGTTAC
CGCACTCAGT CTTCGTCCTG TGAGAGAGAT TCCCCTTCCA CTTCCAGACA GTCCCCAGCT
AATGGTCATA GCAGCACTAA CAATTCTGTT TCGGACTTGC CATCAACTAC CCAACCAAAA
GGACGACAGA TCTATGTAAG AGCACAATTT GAATATGATC CAGCCAAGGA TGACCTCATC
CCCTGTAAAG AAGCTGGCAT TCGATTCAGA GTTGGTGACA TCATCCAGAT TATTAGTAAG
GATGATCATA ATTGGTGGCA GGGTAAACTG GAAAACTCCA AAAATGGAAC TGCAGGTCTC
ATTCCTTCTC CTGAACTTCA GGAATGGCGA GTAGCTTGCA TTGCCATGGA GAAGACCAAA
CAGGAGCAGC AGGCCAGCTG TACTTGGTTT GGCAAGAAAA AGAAGCAGTA CAAAGATAAA
TATTTGGCAA AGCACAATGC AGTGTTTGAT CAATTAGATC TTGTCACATA TGAAGAAGTA
GTAAAACTGC CAGCATTCAA GAGGAAAACA CTAGTCTTAT TAGGCGCACA TGGTGTTGGG
AGAAGACACA TAAAAAACAC TCTCATCACA AAGCACCCAG ACCGGTTTGC GTACCCTATT
CCACATACAA CCAGACCTCC AAAGAAAGAC GAAGAAAATG GAAAGAATTA TTACTTTGTA
TCTCATGACC AAATGATGCA AGACATCTCT AATAACGAGT ACTTGGAGTA CGGCAGCCAC
GAGGATGCGA TGTATGGGAC AAAACTGGAG ACCATCCGGA AGATCCACGA GCAGGGGCTG
ATTGCAATAC TGGACGTGGA GCCTCAGGCA CTGAAGGTCC TGAGAACTGC AGAGTTTGCT
CCTTTTGTTG TTTTCATTGC TGCACCAACT ATTACTCCAG GTTTAAATGA GGATGAATCT
CTTCAGCGTC TGCAGAAGGA GTCTGACATC TTACAGAGAA CATATGCACA CTACTTCGAT
CTCACAATTA TCAACAATGA AATTGATGAG ACAATCAGAC ATCTGGAGGA AGCTGTTGAG
CTCGTGTGCA CAGCCCCACA GTGGGTCCCT GTCTCCTGGG TCTATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011542299.1
CDS128..2335
Misc Feature(1)128..493(+)
Misc Feature(2)128..400(+)
Misc Feature(3)590..745(+)
Misc Feature(4)767..916(+)
Misc Feature(5)1016..1258(+)
Misc Feature(6)1052..1225(+)
Misc Feature(7)1400..1585(+)
Misc Feature(8)1406..1483(+)
Misc Feature(9)1415..1582(+)
Misc Feature(10)1766..2287(+)
Misc Feature(11)1778..2275(+)
Misc Feature(12)1790..2002(+)
Misc Feature(13)1790..1810(+)
Translation

Target ORF information:

RefSeq Version XM_011543997
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543997

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCACCAG AAGTGGTCAA AAGAGAGCCT TACGGAAAGC CTGTAGACGT CTGGGGGTGC 
GGTGTGATCC TTTTTATCCT GCTCAGTGGT TGTTTGCCTT TTTACGGAAC CAAGGAAAGA
TTGTTTGAAG GCATTATTAA AGGAAAATAT AAGATGAATC CAAGGCAGTG GAGCCATATC
TCTGAAAGTG CCAAAGACCT AGTACGTCGC ATGCTGATGC TGGATCCAGC TGAAAGGATC
ACTGTTTATG AAGCACTGAA TCACCCATGG CTTAAGGAGC GGGATCGTTA CGCCTACAAG
ATTCATCTTC CAGAAACAGT AGAGCAGCTG AGGAAATTCA ATGCAAGGAG GAAACTAAAG
GGTGCAGTAC TAGCCGCTGT GTCAAGTCAC AAATTCAACT CATTCTATGG GGATCCCCCT
GAAGAGTTAC CAGATTTCTC CGAAGACCCT ACCTCCTCAG GAGCAGTCTC ACAGGTGCTG
GACAGCCTGG AAGAGATTCA TGCGCTTACA GACTGCAGTG AAAAGGACCT AGATTTTCTA
CACAGTGTTT TCCAGGATCA GCATCTTCAC ACACTACTAG ATCTGTATGA CAAAATTAAC
ACAAAGTCTT CACCACAAAT CAGGAATCCT CCAAGCGATG CAGTACAGAG AGCCAAAGAG
GTATTGGAAG AAATTTCATG TTACCCTGAG AATAACGACG CAAAGGAACT AAAGCGTATT
TTAACACAAC CTCATTTCAT GGCCTTACTT CAGACTCACG ACGTAGTGGC ACATGAAGTT
TACAGTGATG AAGCATTGAG GGTCACACCT CCTCCCACCT CTCCCTATTT AAACGGCGAT
TCTCCAGAAA GTGCTAACGG AGACATGGAT ATGGAGAATG TGACCAGAGT TCGGCTGGTA
CAGTTTCAAA AGAACACAGA TGAACCAATG GGAATCACTT TAAAAATGAA TGAACTAAAT
CATTGTATTG TTGCAAGAAT TATGCATGGG GGCATGATTC ACAGGCAAGG TACACTTCAT
GTTGGTGATG AAATTCGAGA AATCAATGGC ATCAGTGTGG CTAACCAAAC AGTGGAACAA
CTGCAAAAAA TGCTTAGGGA AATGCGGGGG AGTATTACCT TCAAGATTGT GCCAAGTTAC
CGCACTCAGT CTTCGTCCTG TGAGAGAGAT TCCCCTTCCA CTTCCAGACA GTCCCCAGCT
AATGGTCATA GCAGCACTAA CAATTCTGTT TCGGACTTGC CATCAACTAC CCAACCAAAA
GGACGACAGA TCTATGTAAG AGCACAATTT GAATATGATC CAGCCAAGGA TGACCTCATC
CCCTGTAAAG AAGCTGGCAT TCGATTCAGA GTTGGTGACA TCATCCAGAT TATTAGTAAG
GATGATCATA ATTGGTGGCA GGGTAAACTG GAAAACTCCA AAAATGGAAC TGCAGGTCTC
ATTCCTTCTC CTGAACTTCA GGAATGGCGA GTAGCTTGCA TTGCCATGGA GAAGACCAAA
CAGGAGCAGC AGGCCAGCTG TACTTGGTTT GGCAAGAAAA AGAAGCAGTA CAAAGATAAA
TATTTGGCAA AGCACAATGC AGTGTTTGAT CAATTAGATC TTGTCACATA TGAAGAAGTA
GTAAAACTGC CAGCATTCAA GAGGAAAACA CTAGTCTTAT TAGGCGCACA TGGTGTTGGG
AGAAGACACA TAAAAAACAC TCTCATCACA AAGCACCCAG ACCGGTTTGC GTACCCTATT
CCACATACAA CCAGACCTCC AAAGAAAGAC GAAGAAAATG GAAAGAATTA TTACTTTGTA
TCTCATGACC AAATGATGCA AGACATCTCT AATAACGAGT ACTTGGAGTA CGGCAGCCAC
GAGGATGCGA TGTATGGGAC AAAACTGGAG ACCATCCGGA AGATCCACGA GCAGGGGCTG
ATTGCAATAC TGGACGTGGA GCCTCAGGCA CTGAAGGTCC TGAGAACTGC AGAGTTTGCT
CCTTTTGTTG TTTTCATTGC TGCACCAACT ATTACTCCAG GTTTAAATGA GGATGAATCT
CTTCAGCGTC TGCAGAAGGA GTCTGACATC TTACAGAGAA CATATGCACA CTACTTCGAT
CTCACAATTA TCAACAATGA AATTGATGAG ACAATCAGAC ATCTGGAGGA AGCTGTTGAG
CTCGTGTGCA CAGCCCCACA GTGGGTCCCT GTCTCCTGGG TCTATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27417
Clone ID Related Accession (Same CDS sequence) NM_003688
Accession Version NM_003688.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN993605.1, AF032119.1, BC117311.1, AB208859.1 and AL445239.8. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2008 this sequence version replaced gi:186972119. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB208859.1, AF032119.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGACTT
CTAGCAGCAG AAAGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGAGA
GATTCCCCTT CCACTTCCAG ACAGTCCCCA GCTAATGGTC ATAGCAGCAC TAACAATTCT
GTTTCGGACT TGCCATCAAC TACCCAACCA AAAGGACGAC AGATCTATGT AAGAGCACAA
TTTGAATATG ATCCAGCCAA GGATGACCTC ATCCCCTGTA AAGAAGCTGG CATTCGATTC
AGAGTTGGTG ACATCATCCA GATTATTAGT AAGGATGATC ATAATTGGTG GCAGGGTAAA
CTGGAAAACT CCAAAAATGG AACTGCAGGT CTCATTCCTT CTCCTGAACT TCAGGAATGG
CGAGTAGCTT GCATTGCCAT GGAGAAGACC AAACAGGAGC AGCAGGCCAG CTGTACTTGG
TTTGGCAAGA AAAAGAAGCA GTACAAAGAT AAATATTTGG CAAAGCACAA TGCAGATCTT
GTCACATATG AAGAAGTAGT AAAACTGCCA GCATTCAAGA GGAAAACACT AGTCTTATTA
GGCGCACATG GTGTTGGGAG AAGACACATA AAAAACACTC TCATCACAAA GCACCCAGAC
CGGTTTGCGT ACCCTATTCC ACATACAACC AGACCTCCAA AGAAAGACGA AGAAAATGGA
AAGAATTATT ACTTTGTATC TCATGACCAA ATGATGCAAG ACATCTCTAA TAACGAGTAC
TTGGAGTACG GCAGCCACGA GGATGCGATG TATGGGACAA AACTGGAGAC CATCCGGAAG
ATCCACGAGC AGGGGCTGAT TGCAATACTG GACGTGGAGC CTCAGGCACT GAAGGTCCTG
AGAACTGCAG AGTTTGCTCC TTTTGTTGTT TTCATTGCTG CACCAACTAT TACTCCAGGT
TTAAATGAGG ATGAATCTCT TCAGCGTCTG CAGAAGGAGT CTGACATCTT ACAGAGAACA
TATGCACACT ACTTCGATCT CACAATTATC AACAATGAAA TTGATGAGAC AATCAGACAT
CTGGAGGAAG CTGTTGAGCT CGTGTGCACA GCCCCACAGT GGGTCCCTGT CTCCTGGGTC
TATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003679.2
CDS47..2812
Misc Feature(1)68..967(+)
Misc Feature(2)80..874(+)
Misc Feature(3)98..604(+)
Misc Feature(4)98..532(+)
Misc Feature(5)110..604(+)
Misc Feature(6)497..499(+)
Misc Feature(7)509..511(+)
Misc Feature(8)527..598(+)
Misc Feature(9)959..991(+)
Misc Feature(10)1082..1237(+)
Misc Feature(11)1259..1408(+)
Misc Feature(12)1508..1750(+)
Misc Feature(13)1544..1717(+)
Misc Feature(14)1892..2077(+)
Misc Feature(15)1898..1975(+)
Misc Feature(16)1907..2074(+)
Misc Feature(17)2243..2764(+)
Misc Feature(18)2255..2752(+)
Misc Feature(19)2267..2479(+)
Misc Feature(20)2267..2287(+)
Exon (1)1..105
Gene:CASK
Gene Synonym:
Exon (2)106..218
Gene:CASK
Gene Synonym:
Exon (3)219..324
Gene:CASK
Gene Synonym:
Exon (4)325..402
Gene:CASK
Gene Synonym:
Exon (5)403..475
Gene:CASK
Gene Synonym:
Exon (6)476..578
Gene:CASK
Gene Synonym:
Exon (7)579..754
Gene:CASK
Gene Synonym:
Exon (8)755..877
Gene:CASK
Gene Synonym:
Exon (9)878..961
Gene:CASK
Gene Synonym:
Exon (10)962..1061
Gene:CASK
Gene Synonym:
Exon (11)1062..1079
Gene:CASK
Gene Synonym:
Exon (12)1080..1201
Gene:CASK
Gene Synonym:
Exon (13)1202..1279
Gene:CASK
Gene Synonym:
Exon (14)1280..1360
Gene:CASK
Gene Synonym:
Exon (15)1361..1549
Gene:CASK
Gene Synonym:
Exon (16)1550..1628
Gene:CASK
Gene Synonym:
Exon (17)1629..1714
Gene:CASK
Gene Synonym:
Exon (18)1715..1783
Gene:CASK
Gene Synonym:
Exon (19)1784..1852
Gene:CASK
Gene Synonym:
Exon (20)1853..1888
Gene:CASK
Gene Synonym:
Exon (21)1889..2085
Gene:CASK
Gene Synonym:
Exon (22)2086..2201
Gene:CASK
Gene Synonym:
Exon (23)2202..2267
Gene:CASK
Gene Synonym:
Exon (24)2268..2348
Gene:CASK
Gene Synonym:
Exon (25)2349..2551
Gene:CASK
Gene Synonym:
Exon (26)2552..2635
Gene:CASK
Gene Synonym:
Exon (27)2636..8298
Gene:CASK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_003688
Organism Homo sapiens (human)
Definition Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003688

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGACTT
CTAGCAGCAG AAAGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGAGA
GATTCCCCTT CCACTTCCAG ACAGTCCCCA GCTAATGGTC ATAGCAGCAC TAACAATTCT
GTTTCGGACT TGCCATCAAC TACCCAACCA AAAGGACGAC AGATCTATGT AAGAGCACAA
TTTGAATATG ATCCAGCCAA GGATGACCTC ATCCCCTGTA AAGAAGCTGG CATTCGATTC
AGAGTTGGTG ACATCATCCA GATTATTAGT AAGGATGATC ATAATTGGTG GCAGGGTAAA
CTGGAAAACT CCAAAAATGG AACTGCAGGT CTCATTCCTT CTCCTGAACT TCAGGAATGG
CGAGTAGCTT GCATTGCCAT GGAGAAGACC AAACAGGAGC AGCAGGCCAG CTGTACTTGG
TTTGGCAAGA AAAAGAAGCA GTACAAAGAT AAATATTTGG CAAAGCACAA TGCAGATCTT
GTCACATATG AAGAAGTAGT AAAACTGCCA GCATTCAAGA GGAAAACACT AGTCTTATTA
GGCGCACATG GTGTTGGGAG AAGACACATA AAAAACACTC TCATCACAAA GCACCCAGAC
CGGTTTGCGT ACCCTATTCC ACATACAACC AGACCTCCAA AGAAAGACGA AGAAAATGGA
AAGAATTATT ACTTTGTATC TCATGACCAA ATGATGCAAG ACATCTCTAA TAACGAGTAC
TTGGAGTACG GCAGCCACGA GGATGCGATG TATGGGACAA AACTGGAGAC CATCCGGAAG
ATCCACGAGC AGGGGCTGAT TGCAATACTG GACGTGGAGC CTCAGGCACT GAAGGTCCTG
AGAACTGCAG AGTTTGCTCC TTTTGTTGTT TTCATTGCTG CACCAACTAT TACTCCAGGT
TTAAATGAGG ATGAATCTCT TCAGCGTCTG CAGAAGGAGT CTGACATCTT ACAGAGAACA
TATGCACACT ACTTCGATCT CACAATTATC AACAATGAAA TTGATGAGAC AATCAGACAT
CTGGAGGAAG CTGTTGAGCT CGTGTGCACA GCCCCACAGT GGGTCCCTGT CTCCTGGGTC
TATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27080
Clone ID Related Accession (Same CDS sequence) NM_001126054
Accession Version NM_001126054.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2697bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN993605.1, BC117311.1, AB208859.1 and AL445239.8. On Jul 3, 2008 this sequence version replaced gi:186700626. Transcript Variant: Transcript Variant: This variant (2) contains an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC117311.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGACTT
CTAGCAGCAG AAAGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGGAC
TTGCCATCAA CTACCCAACC AAAAGGACGA CAGATCTATG TAAGAGCACA ATTTGAATAT
GATCCAGCCA AGGATGACCT CATCCCCTGT AAAGAAGCTG GCATTCGATT CAGAGTTGGT
GACATCATCC AGATTATTAG TAAGGATGAT CATAATTGGT GGCAGGGTAA ACTGGAAAAC
TCCAAAAATG GAACTGCAGG TCTCATTCCT TCTCCTGAAC TTCAGGAATG GCGAGTAGCT
TGCATTGCCA TGGAGAAGAC CAAACAGGAG CAGCAGGCCA GCTGTACTTG GTTTGGCAAG
AAAAAGAAGC AGTACAAAGA TAAATATTTG GCAAAGCACA ATGCAGATCT TGTCACATAT
GAAGAAGTAG TAAAACTGCC AGCATTCAAG AGGAAAACAC TAGTCTTATT AGGCGCACAT
GGTGTTGGGA GAAGACACAT AAAAAACACT CTCATCACAA AGCACCCAGA CCGGTTTGCG
TACCCTATTC CACATACAAC CAGACCTCCA AAGAAAGACG AAGAAAATGG AAAGAATTAT
TACTTTGTAT CTCATGACCA AATGATGCAA GACATCTCTA ATAACGAGTA CTTGGAGTAC
GGCAGCCACG AGGATGCGAT GTATGGGACA AAACTGGAGA CCATCCGGAA GATCCACGAG
CAGGGGCTGA TTGCAATACT GGACGTGGAG CCTCAGGCAC TGAAGGTCCT GAGAACTGCA
GAGTTTGCTC CTTTTGTTGT TTTCATTGCT GCACCAACTA TTACTCCAGG TTTAAATGAG
GATGAATCTC TTCAGCGTCT GCAGAAGGAG TCTGACATCT TACAGAGAAC ATATGCACAC
TACTTCGATC TCACAATTAT CAACAATGAA ATTGATGAGA CAATCAGACA TCTGGAGGAA
GCTGTTGAGC TCGTGTGCAC AGCCCCACAG TGGGTCCCTG TCTCCTGGGT CTATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119526.1
CDS47..2743
Misc Feature(1)68..967(+)
Misc Feature(2)80..874(+)
Misc Feature(3)98..604(+)
Misc Feature(4)98..532(+)
Misc Feature(5)110..604(+)
Misc Feature(6)527..598(+)
Misc Feature(7)1082..1237(+)
Misc Feature(8)1259..1408(+)
Misc Feature(9)1508..1750(+)
Misc Feature(10)1544..1717(+)
Misc Feature(11)1823..2008(+)
Misc Feature(12)1829..1906(+)
Misc Feature(13)1838..2005(+)
Misc Feature(14)2174..2695(+)
Misc Feature(15)2186..2683(+)
Misc Feature(16)2198..2410(+)
Misc Feature(17)2198..2218(+)
Exon (1)1..105
Gene:CASK
Gene Synonym:
Exon (2)106..218
Gene:CASK
Gene Synonym:
Exon (3)219..324
Gene:CASK
Gene Synonym:
Exon (4)325..402
Gene:CASK
Gene Synonym:
Exon (5)403..475
Gene:CASK
Gene Synonym:
Exon (6)476..578
Gene:CASK
Gene Synonym:
Exon (7)579..754
Gene:CASK
Gene Synonym:
Exon (8)755..877
Gene:CASK
Gene Synonym:
Exon (9)878..961
Gene:CASK
Gene Synonym:
Exon (10)962..1061
Gene:CASK
Gene Synonym:
Exon (11)1062..1079
Gene:CASK
Gene Synonym:
Exon (12)1080..1201
Gene:CASK
Gene Synonym:
Exon (13)1202..1279
Gene:CASK
Gene Synonym:
Exon (14)1280..1360
Gene:CASK
Gene Synonym:
Exon (15)1361..1549
Gene:CASK
Gene Synonym:
Exon (16)1550..1628
Gene:CASK
Gene Synonym:
Exon (17)1629..1714
Gene:CASK
Gene Synonym:
Exon (18)1715..1783
Gene:CASK
Gene Synonym:
Exon (19)1784..1819
Gene:CASK
Gene Synonym:
Exon (20)1820..2016
Gene:CASK
Gene Synonym:
Exon (21)2017..2132
Gene:CASK
Gene Synonym:
Exon (22)2133..2198
Gene:CASK
Gene Synonym:
Exon (23)2199..2279
Gene:CASK
Gene Synonym:
Exon (24)2280..2482
Gene:CASK
Gene Synonym:
Exon (25)2483..2566
Gene:CASK
Gene Synonym:
Exon (26)2567..8229
Gene:CASK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126054
Organism Homo sapiens (human)
Definition Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126054

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGACTT
CTAGCAGCAG AAAGAGCAGT CTCACAGGTG CTGGACAGCC TGGAAGAGAT TCATGCGCTT
ACAGACTGCA GTGAAAAGGA CCTAGATTTT CTACACAGTG TTTTCCAGGA TCAGCATCTT
CACACACTAC TAGATCTGTA TGACAAAATT AACACAAAGT CTTCACCACA AATCAGGAAT
CCTCCAAGCG ATGCAGTACA GAGAGCCAAA GAGGTATTGG AAGAAATTTC ATGTTACCCT
GAGAATAACG ACGCAAAGGA ACTAAAGCGT ATTTTAACAC AACCTCATTT CATGGCCTTA
CTTCAGACTC ACGACGTAGT GGCACATGAA GTTTACAGTG ATGAAGCATT GAGGGTCACA
CCTCCTCCCA CCTCTCCCTA TTTAAACGGC GATTCTCCAG AAAGTGCTAA CGGAGACATG
GATATGGAGA ATGTGACCAG AGTTCGGCTG GTACAGTTTC AAAAGAACAC AGATGAACCA
ATGGGAATCA CTTTAAAAAT GAATGAACTA AATCATTGTA TTGTTGCAAG AATTATGCAT
GGGGGCATGA TTCACAGGCA AGGTACACTT CATGTTGGTG ATGAAATTCG AGAAATCAAT
GGCATCAGTG TGGCTAACCA AACAGTGGAA CAACTGCAAA AAATGCTTAG GGAAATGCGG
GGGAGTATTA CCTTCAAGAT TGTGCCAAGT TACCGCACTC AGTCTTCGTC CTGTGAGGAC
TTGCCATCAA CTACCCAACC AAAAGGACGA CAGATCTATG TAAGAGCACA ATTTGAATAT
GATCCAGCCA AGGATGACCT CATCCCCTGT AAAGAAGCTG GCATTCGATT CAGAGTTGGT
GACATCATCC AGATTATTAG TAAGGATGAT CATAATTGGT GGCAGGGTAA ACTGGAAAAC
TCCAAAAATG GAACTGCAGG TCTCATTCCT TCTCCTGAAC TTCAGGAATG GCGAGTAGCT
TGCATTGCCA TGGAGAAGAC CAAACAGGAG CAGCAGGCCA GCTGTACTTG GTTTGGCAAG
AAAAAGAAGC AGTACAAAGA TAAATATTTG GCAAAGCACA ATGCAGATCT TGTCACATAT
GAAGAAGTAG TAAAACTGCC AGCATTCAAG AGGAAAACAC TAGTCTTATT AGGCGCACAT
GGTGTTGGGA GAAGACACAT AAAAAACACT CTCATCACAA AGCACCCAGA CCGGTTTGCG
TACCCTATTC CACATACAAC CAGACCTCCA AAGAAAGACG AAGAAAATGG AAAGAATTAT
TACTTTGTAT CTCATGACCA AATGATGCAA GACATCTCTA ATAACGAGTA CTTGGAGTAC
GGCAGCCACG AGGATGCGAT GTATGGGACA AAACTGGAGA CCATCCGGAA GATCCACGAG
CAGGGGCTGA TTGCAATACT GGACGTGGAG CCTCAGGCAC TGAAGGTCCT GAGAACTGCA
GAGTTTGCTC CTTTTGTTGT TTTCATTGCT GCACCAACTA TTACTCCAGG TTTAAATGAG
GATGAATCTC TTCAGCGTCT GCAGAAGGAG TCTGACATCT TACAGAGAAC ATATGCACAC
TACTTCGATC TCACAATTAT CAACAATGAA ATTGATGAGA CAATCAGACA TCTGGAGGAA
GCTGTTGAGC TCGTGTGCAC AGCCCCACAG TGGGTCCCTG TCTCCTGGGT CTATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26584
Clone ID Related Accession (Same CDS sequence) NM_001126055
Accession Version NM_001126055.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product peripheral plasma membrane protein CASK isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313399.1, AF035582.1, BC117311.1, AB208859.1 and AL445239.8. On Jul 3, 2008 this sequence version replaced gi:186700628. Transcript Variant: This variant (3) lacks two alternate in-frame exons and use an alternate in-frame splice site compared to variant 1. The resulting protein (isoform 3) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF035582.1, BC143456.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGAGCA
GTCTCACAGG TGCTGGACAG CCTGGAAGAG ATTCATGCGC TTACAGACTG CAGTGAAAAG
GACCTAGATT TTCTACACAG TGTTTTCCAG GATCAGCATC TTCACACACT ACTAGATCTG
TATGACAAAA TTAACACAAA GTCTTCACCA CAAATCAGGA ATCCTCCAAG CGATGCAGTA
CAGAGAGCCA AAGAGGTATT GGAAGAAATT TCATGTTACC CTGAGAATAA CGACGCAAAG
GAACTAAAGC GTATTTTAAC ACAACCTCAT TTCATGGCCT TACTTCAGAC TCACGACGTA
GTGGCACATG AAGTTTACAG TGATGAAGCA TTGAGGGTCA CACCTCCTCC CACCTCTCCC
TATTTAAACG GCGATTCTCC AGAAAGTGCT AACGGAGACA TGGATATGGA GAATGTGACC
AGAGTTCGGC TGGTACAGTT TCAAAAGAAC ACAGATGAAC CAATGGGAAT CACTTTAAAA
ATGAATGAAC TAAATCATTG TATTGTTGCA AGAATTATGC ATGGGGGCAT GATTCACAGG
CAAGGTACAC TTCATGTTGG TGATGAAATT CGAGAAATCA ATGGCATCAG TGTGGCTAAC
CAAACAGTGG AACAACTGCA AAAAATGCTT AGGGAAATGC GGGGGAGTAT TACCTTCAAG
ATTGTGCCAA GTTACCGCAC TCAGTCTTCG TCCTGTGAGG ACTTGCCATC AACTACCCAA
CCAAAAGGAC GACAGATCTA TGTAAGAGCA CAATTTGAAT ATGATCCAGC CAAGGATGAC
CTCATCCCCT GTAAAGAAGC TGGCATTCGA TTCAGAGTTG GTGACATCAT CCAGATTATT
AGTAAGGATG ATCATAATTG GTGGCAGGGT AAACTGGAAA ACTCCAAAAA TGGAACTGCA
GGTCTCATTC CTTCTCCTGA ACTTCAGGAA TGGCGAGTAG CTTGCATTGC CATGGAGAAG
ACCAAACAGG AGCAGCAGGC CAGCTGTACT TGGTTTGGCA AGAAAAAGAA GCAGTACAAA
GATAAATATT TGGCAAAGCA CAATGCAGTG TTTGATCAAT TAGATCTTGT CACATATGAA
GAAGTAGTAA AACTGCCAGC ATTCAAGAGG AAAACACTAG TCTTATTAGG CGCACATGGT
GTTGGGAGAA GACACATAAA AAACACTCTC ATCACAAAGC ACCCAGACCG GTTTGCGTAC
CCTATTCCAC ATACAACCAG ACCTCCAAAG AAAGACGAAG AAAATGGAAA GAATTATTAC
TTTGTATCTC ATGACCAAAT GATGCAAGAC ATCTCTAATA ACGAGTACTT GGAGTACGGC
AGCCACGAGG ATGCGATGTA TGGGACAAAA CTGGAGACCA TCCGGAAGAT CCACGAGCAG
GGGCTGATTG CAATACTGGA CGTGGAGCCT CAGGCACTGA AGGTCCTGAG AACTGCAGAG
TTTGCTCCTT TTGTTGTTTT CATTGCTGCA CCAACTATTA CTCCAGGTTT AAATGAGGAT
GAATCTCTTC AGCGTCTGCA GAAGGAGTCT GACATCTTAC AGAGAACATA TGCACACTAC
TTCGATCTCA CAATTATCAA CAATGAAATT GATGAGACAA TCAGACATCT GGAGGAAGCT
GTTGAGCTCG TGTGCACAGC CCCACAGTGG GTCCCTGTCT CCTGGGTCTA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001119527.1
CDS47..2740
Misc Feature(1)68..967(+)
Misc Feature(2)80..874(+)
Misc Feature(3)98..604(+)
Misc Feature(4)98..532(+)
Misc Feature(5)110..604(+)
Misc Feature(6)527..598(+)
Misc Feature(7)1064..1219(+)
Misc Feature(8)1241..1390(+)
Misc Feature(9)1490..1732(+)
Misc Feature(10)1526..1699(+)
Misc Feature(11)1805..1990(+)
Misc Feature(12)1811..1888(+)
Misc Feature(13)1820..1987(+)
Misc Feature(14)2171..2692(+)
Misc Feature(15)2183..2680(+)
Misc Feature(16)2195..2407(+)
Misc Feature(17)2195..2215(+)
Exon (1)1..105
Gene:CASK
Gene Synonym:
Exon (2)106..218
Gene:CASK
Gene Synonym:
Exon (3)219..324
Gene:CASK
Gene Synonym:
Exon (4)325..402
Gene:CASK
Gene Synonym:
Exon (5)403..475
Gene:CASK
Gene Synonym:
Exon (6)476..578
Gene:CASK
Gene Synonym:
Exon (7)579..754
Gene:CASK
Gene Synonym:
Exon (8)755..877
Gene:CASK
Gene Synonym:
Exon (9)878..961
Gene:CASK
Gene Synonym:
Exon (10)962..1061
Gene:CASK
Gene Synonym:
Exon (11)1062..1183
Gene:CASK
Gene Synonym:
Exon (12)1184..1261
Gene:CASK
Gene Synonym:
Exon (13)1262..1342
Gene:CASK
Gene Synonym:
Exon (14)1343..1531
Gene:CASK
Gene Synonym:
Exon (15)1532..1610
Gene:CASK
Gene Synonym:
Exon (16)1611..1696
Gene:CASK
Gene Synonym:
Exon (17)1697..1765
Gene:CASK
Gene Synonym:
Exon (18)1766..1801
Gene:CASK
Gene Synonym:
Exon (19)1802..1998
Gene:CASK
Gene Synonym:
Exon (20)1999..2114
Gene:CASK
Gene Synonym:
Exon (21)2115..2195
Gene:CASK
Gene Synonym:
Exon (22)2196..2276
Gene:CASK
Gene Synonym:
Exon (23)2277..2479
Gene:CASK
Gene Synonym:
Exon (24)2480..2563
Gene:CASK
Gene Synonym:
Exon (25)2564..8226
Gene:CASK
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001126055
Organism Homo sapiens (human)
Definition Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001126055

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGGCCGACG ACGACGTGCT GTTCGAGGAT GTGTACGAGC TGTGCGAGGT GATCGGAAAG 
GGTCCCTTCA GTGTTGTACG ACGATGTATC AACAGAGAAA CTGGGCAACA ATTTGCTGTA
AAAATTGTTG ATGTAGCCAA GTTCACATCA AGTCCAGGGT TAAGTACAGA AGATCTAAAG
CGGGAAGCCA GTATCTGTCA TATGCTGAAA CATCCACACA TTGTAGAGTT ATTGGAGACA
TATAGCTCAG ATGGAATGCT TTACATGGTT TTCGAATTTA TGGATGGAGC AGATCTGTGT
TTTGAAATCG TAAAGCGAGC TGACGCTGGT TTTGTGTACA GTGAAGCTGT AGCCAGCCAT
TATATGAGAC AGATACTGGA AGCTCTACGC TACTGCCATG ATAATAACAT AATTCACAGG
GATGTGAAGC CCCACTGTGT TCTCCTTGCC TCAAAAGAAA ACTCGGCACC TGTTAAACTT
GGAGGCTTTG GGGTAGCTAT TCAATTAGGG GAGTCTGGAC TTGTAGCTGG AGGACGTGTT
GGAACACCTC ATTTTATGGC ACCAGAAGTG GTCAAAAGAG AGCCTTACGG AAAGCCTGTA
GACGTCTGGG GGTGCGGTGT GATCCTTTTT ATCCTGCTCA GTGGTTGTTT GCCTTTTTAC
GGAACCAAGG AAAGATTGTT TGAAGGCATT ATTAAAGGAA AATATAAGAT GAATCCAAGG
CAGTGGAGCC ATATCTCTGA AAGTGCCAAA GACCTAGTAC GTCGCATGCT GATGCTGGAT
CCAGCTGAAA GGATCACTGT TTATGAAGCA CTGAATCACC CATGGCTTAA GGAGCGGGAT
CGTTACGCCT ACAAGATTCA TCTTCCAGAA ACAGTAGAGC AGCTGAGGAA ATTCAATGCA
AGGAGGAAAC TAAAGGGTGC AGTACTAGCC GCTGTGTCAA GTCACAAATT CAACTCATTC
TATGGGGATC CCCCTGAAGA GTTACCAGAT TTCTCCGAAG ACCCTACCTC CTCAGGAGCA
GTCTCACAGG TGCTGGACAG CCTGGAAGAG ATTCATGCGC TTACAGACTG CAGTGAAAAG
GACCTAGATT TTCTACACAG TGTTTTCCAG GATCAGCATC TTCACACACT ACTAGATCTG
TATGACAAAA TTAACACAAA GTCTTCACCA CAAATCAGGA ATCCTCCAAG CGATGCAGTA
CAGAGAGCCA AAGAGGTATT GGAAGAAATT TCATGTTACC CTGAGAATAA CGACGCAAAG
GAACTAAAGC GTATTTTAAC ACAACCTCAT TTCATGGCCT TACTTCAGAC TCACGACGTA
GTGGCACATG AAGTTTACAG TGATGAAGCA TTGAGGGTCA CACCTCCTCC CACCTCTCCC
TATTTAAACG GCGATTCTCC AGAAAGTGCT AACGGAGACA TGGATATGGA GAATGTGACC
AGAGTTCGGC TGGTACAGTT TCAAAAGAAC ACAGATGAAC CAATGGGAAT CACTTTAAAA
ATGAATGAAC TAAATCATTG TATTGTTGCA AGAATTATGC ATGGGGGCAT GATTCACAGG
CAAGGTACAC TTCATGTTGG TGATGAAATT CGAGAAATCA ATGGCATCAG TGTGGCTAAC
CAAACAGTGG AACAACTGCA AAAAATGCTT AGGGAAATGC GGGGGAGTAT TACCTTCAAG
ATTGTGCCAA GTTACCGCAC TCAGTCTTCG TCCTGTGAGG ACTTGCCATC AACTACCCAA
CCAAAAGGAC GACAGATCTA TGTAAGAGCA CAATTTGAAT ATGATCCAGC CAAGGATGAC
CTCATCCCCT GTAAAGAAGC TGGCATTCGA TTCAGAGTTG GTGACATCAT CCAGATTATT
AGTAAGGATG ATCATAATTG GTGGCAGGGT AAACTGGAAA ACTCCAAAAA TGGAACTGCA
GGTCTCATTC CTTCTCCTGA ACTTCAGGAA TGGCGAGTAG CTTGCATTGC CATGGAGAAG
ACCAAACAGG AGCAGCAGGC CAGCTGTACT TGGTTTGGCA AGAAAAAGAA GCAGTACAAA
GATAAATATT TGGCAAAGCA CAATGCAGTG TTTGATCAAT TAGATCTTGT CACATATGAA
GAAGTAGTAA AACTGCCAGC ATTCAAGAGG AAAACACTAG TCTTATTAGG CGCACATGGT
GTTGGGAGAA GACACATAAA AAACACTCTC ATCACAAAGC ACCCAGACCG GTTTGCGTAC
CCTATTCCAC ATACAACCAG ACCTCCAAAG AAAGACGAAG AAAATGGAAA GAATTATTAC
TTTGTATCTC ATGACCAAAT GATGCAAGAC ATCTCTAATA ACGAGTACTT GGAGTACGGC
AGCCACGAGG ATGCGATGTA TGGGACAAAA CTGGAGACCA TCCGGAAGAT CCACGAGCAG
GGGCTGATTG CAATACTGGA CGTGGAGCCT CAGGCACTGA AGGTCCTGAG AACTGCAGAG
TTTGCTCCTT TTGTTGTTTT CATTGCTGCA CCAACTATTA CTCCAGGTTT AAATGAGGAT
GAATCTCTTC AGCGTCTGCA GAAGGAGTCT GACATCTTAC AGAGAACATA TGCACACTAC
TTCGATCTCA CAATTATCAA CAATGAAATT GATGAGACAA TCAGACATCT GGAGGAAGCT
GTTGAGCTCG TGTGCACAGC CCCACAGTGG GTCCCTGTCT CCTGGGTCTA TTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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