CHRD cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following CHRD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18610 NM_003741.3
Latest version!
Homo sapiens chordin (CHRD), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu62092 NM_001304474.2
Latest version!
Homo sapiens chordin (CHRD), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu62092 NM_001304473.2
Latest version!
Homo sapiens chordin (CHRD), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu62092 XM_024453803.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu62092 XM_011513256.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu94718 XM_017007394.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu38216 NM_001304472.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu62090 XM_011513254.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94712 XM_017007388.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94713 XM_017007389.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94714 XM_017007390.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94715 XM_017007391.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94716 XM_017007392.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $475.30
$679.00
OHu94717 XM_017007393.1
Latest version!
Homo sapiens chordin (CHRD), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $391.30
$559.00
View Old Accession Versions >>
OHu62092 NM_001304474.1 Homo sapiens chordin (CHRD), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu62092 NM_001304473.1 Homo sapiens chordin (CHRD), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18610
    Clone ID Related Accession (Same CDS sequence) NM_003741.3
    Accession Version NM_003741.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Homo sapiens(human)
    Product chordin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209928.1, BC112345.1 and BC029879.1. This sequence is a reference standard in the RefSeqGene project. On Feb 3, 2015 this sequence version replaced NM_003741.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF209928.1, SRR1803616.14551.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGGTGCACAT AGCCAACCAA TGTGAGGTTG GCGGACTGCG CCTGGAGGCG
    GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG GCTCCGGATA CAGCCTCTGC TGCGCCGCCT
    GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC GCCAAACCTG GTGGTCCTGG GCGGCCCCGA
    GACCCCAACA CATGCTTCTT CGAGGGGCAG CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC
    AACTACGACC CGCTCTGCTC ACTCTGCACC TGCCAGAGAC GAACGGTGAT CTGTGACCCG
    GTGGTGTGCC CACCGCCCAG CTGCCCACAC CCGGTGCAGG CTCCCGACCA GTGCTGCCCT
    GTTTGCCCTG AGAAACAAGA TGTCAGAGAC TTGCCAGGGC TGCCAAGGAG CCGGGACCCA
    GGAGAGGGCT GCTATTTTGA TGGTGACCGG AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC
    CCCGTTGTGC CCCCCTTTGG CTTAATTAAG TGTGCTGTCT GCACCTGCAA GGGGGGCACT
    GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT
    GTCAACCCCA CCGACTGCTG CAAACAGTGT CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG
    GGGGACCCCA TGCAGGCTGA TGGGCCCCGG GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA
    GAGAGTCAGA GCTGGCACCC CTCAGTGCCC CCTTTTGGAG AGATGAGCTG TATCACCTGC
    AGATGTGGGG CAGGGGTGCC TCACTGTGAG CGGGATGACT GTTCACTGCC ACTGTCCTGT
    GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC CGCTGCACGG CCCACCGGCG GCCAGCCCCA
    GAGACCAGAA CTGATCCAGA GCTGGAGAAA GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003732.2
    CDS247..3114
    Translation

    Target ORF information:

    RefSeq Version NM_003741.3
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003741.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGGTGCACAT AGCCAACCAA TGTGAGGTTG GCGGACTGCG CCTGGAGGCG
    GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG GCTCCGGATA CAGCCTCTGC TGCGCCGCCT
    GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC GCCAAACCTG GTGGTCCTGG GCGGCCCCGA
    GACCCCAACA CATGCTTCTT CGAGGGGCAG CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC
    AACTACGACC CGCTCTGCTC ACTCTGCACC TGCCAGAGAC GAACGGTGAT CTGTGACCCG
    GTGGTGTGCC CACCGCCCAG CTGCCCACAC CCGGTGCAGG CTCCCGACCA GTGCTGCCCT
    GTTTGCCCTG AGAAACAAGA TGTCAGAGAC TTGCCAGGGC TGCCAAGGAG CCGGGACCCA
    GGAGAGGGCT GCTATTTTGA TGGTGACCGG AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC
    CCCGTTGTGC CCCCCTTTGG CTTAATTAAG TGTGCTGTCT GCACCTGCAA GGGGGGCACT
    GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT
    GTCAACCCCA CCGACTGCTG CAAACAGTGT CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG
    GGGGACCCCA TGCAGGCTGA TGGGCCCCGG GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA
    GAGAGTCAGA GCTGGCACCC CTCAGTGCCC CCTTTTGGAG AGATGAGCTG TATCACCTGC
    AGATGTGGGG CAGGGGTGCC TCACTGTGAG CGGGATGACT GTTCACTGCC ACTGTCCTGT
    GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC CGCTGCACGG CCCACCGGCG GCCAGCCCCA
    GAGACCAGAA CTGATCCAGA GCTGGAGAAA GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62092
    Clone ID Related Accession (Same CDS sequence) NM_001304474.1 , XM_011513256.1 , NM_001304473.2 , NM_001304474.2 , XM_024453803.1 , NM_001304473.1
    Accession Version NM_001304474.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product chordin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209928.1, BC112345.1, AF283325.1, AY358926.2 and BC029879.1. Transcript Variant: This variant (4) uses an alternate splice site in its 5' UTR, compared to variant 1. This differences causes translation initiation at a downstream start codon and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF283325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291403.1
    CDS1328..3085
    Translation

    Target ORF information:

    RefSeq Version NM_001304474.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62092
    Clone ID Related Accession (Same CDS sequence) NM_001304474.1 , XM_011513256.1 , NM_001304473.2 , NM_001304474.2 , XM_024453803.1 , NM_001304473.1
    Accession Version NM_001304473.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-28
    Organism Homo sapiens(human)
    Product chordin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209928.1, BC112345.1, AF209930.1, AY358926.2, BC029879.1 and AC078797.20. On Jun 2, 2019 this sequence version replaced NM_001304473.1. Transcript Variant: This variant (3) contains an alternate exon and uses an alternate splice site in its 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF209930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291402.1
    CDS1146..2903
    Translation

    Target ORF information:

    RefSeq Version NM_001304473.2
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304473.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62092
    Clone ID Related Accession (Same CDS sequence) NM_001304474.1 , XM_011513256.1 , NM_001304473.2 , NM_001304474.2 , XM_024453803.1 , NM_001304473.1
    Accession Version NM_001304474.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-29
    Organism Homo sapiens(human)
    Product chordin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209928.1, BC112345.1, AF283325.1, AY358926.2, BC029879.1 and AC078797.20. On Jun 2, 2019 this sequence version replaced NM_001304474.1. Transcript Variant: This variant (4) uses an alternate splice site in its 5' UTR, compared to variant 1. This differences causes translation initiation at a downstream start codon and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF283325.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291403.1
    CDS1091..2848
    Translation

    Target ORF information:

    RefSeq Version NM_001304474.2
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304474.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62092
    Clone ID Related Accession (Same CDS sequence) NM_001304474.1 , XM_011513256.1 , NM_001304473.2 , NM_001304474.2 , XM_024453803.1 , NM_001304473.1
    Accession Version NM_001304473.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product chordin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209928.1, BC112345.1, AF209930.1, AY358926.2 and BC029879.1. Transcript Variant: This variant (3) contains an alternate exon and uses an alternate splice site in its 5' UTR, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF209930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291402.1
    CDS1383..3140
    Translation

    Target ORF information:

    RefSeq Version NM_001304473.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304473.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62092
    Clone ID Related Accession (Same CDS sequence) NM_001304474.1 , XM_011513256.1 , NM_001304473.2 , NM_001304474.2 , XM_024453803.1 , NM_001304473.1
    Accession Version XM_011513256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product chordin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511558.1
    CDS1366..3123
    Translation

    Target ORF information:

    RefSeq Version XM_011513256.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62092
    Clone ID Related Accession (Same CDS sequence) NM_001304474.1 , XM_011513256.1 , NM_001304473.2 , NM_001304474.2 , XM_024453803.1 , NM_001304473.1
    Accession Version XM_024453803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1758bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309571.1
    CDS1456..3213
    Translation

    Target ORF information:

    RefSeq Version XM_024453803.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGACTGGC TGGTGCTGGG GGAGCTGCAG ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG 
    CTGCGCATCA GTGGACACAT TGCTGCCAGG AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT
    TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG ACGGGTGCTG CCGGCTCAGC CAGCCTCACG
    CTGCTAGGAA ATGGCTCCCT GATCTATCAG GTGCAAGTGG TAGGGACAAG CAGTGAGGTG
    GTGGCCATGA CACTGGAGAC CAAGCCTCAG CGGAGGGATC AGCGCACTGT CCTGTGCCAC
    ATGGCTGGAC TCCAGCCAGG AGGACACACG GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC
    CGAGGGGCTC ATATGCTGCT GCAGAATGAG CTCTTCCTGA ACGTGGGCAC CAAGGACTTC
    CCAGACGGAG AGCTTCGGGG GCACGTGGCT GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC
    CATGACACGC TGCCCGTGCC CCTAGCAGGA GCCCTGGTGC TACCCCCTGT GAAGAGCCAA
    GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT ACCCACTGTC ACCTGCACTA TGAAGTGCTG
    CTGGCTGGGC TTGGTGGCTC AGAACAAGGC ACTGTCACTG CCCACCTCCT TGGGCCTCCT
    GGAACGCCAG GGCCTCGGCG GCTGCTGAAG GGATTCTATG GCTCAGAGGC CCAGGGTGTG
    GTGAAGGACC TGGAGCCGGA ACTGCTGCGG CACCTGGCAA AAGGCATGGC CTCCCTGATG
    ATCACCACCA AGGGTAGCCC CAGAGGGGAG CTCCGAGGGC AGGTGCACAT AGCCAACCAA
    TGTGAGGTTG GCGGACTGCG CCTGGAGGCG GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG
    GCTCCGGATA CAGCCTCTGC TGCGCCGCCT GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC
    GCCAAACCTG GTGGTCCTGG GCGGCCCCGA GACCCCAACA CATGCTTCTT CGAGGGGCAG
    CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC AACTACGACC CGCTCTGCTC ACTCTGCACC
    TGCCAGAGAC GAACGGTGAT CTGTGACCCG GTGGTGTGCC CACCGCCCAG CTGCCCACAC
    CCGGTGCAGG CTCCCGACCA GTGCTGCCCT GTTTGCCCTG AGAAACAAGA TGTCAGAGAC
    TTGCCAGGGC TGCCAAGGAG CCGGGACCCA GGAGAGGGCT GCTATTTTGA TGGTGACCGG
    AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC CCCGTTGTGC CCCCCTTTGG CTTAATTAAG
    TGTGCTGTCT GCACCTGCAA GGGGGGCACT GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT
    CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT GTCAACCCCA CCGACTGCTG CAAACAGTGT
    CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG GGGGACCCCA TGCAGGCTGA TGGGCCCCGG
    GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA GAGAGTCAGA GCTGGCACCC CTCAGTGCCC
    CCTTTTGGAG AGATGAGCTG TATCACCTGC AGATGTGGGG CAGGGGTGCC TCACTGTGAG
    CGGGATGACT GTTCACTGCC ACTGTCCTGT GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC
    CGCTGCACGG CCCACCGGCG GCCAGCCCCA GAGACCAGAA CTGATCCAGA GCTGGAGAAA
    GAAGCCGAAG GCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94718
    Clone ID Related Accession (Same CDS sequence) XM_017007394.1
    Accession Version XM_017007394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTGCGGCC TTGCAGGACT AACCCAGGTT CCCTTGAGGC TCCAGATTCT ACACCAGGGG 
    CAGCTACTGC GAGAACTTCA GGCCAATGTC TCAGCCCAGG AACCAGGCTT TGCTGAGGTG
    CTGCCCAACC TGACAGTCCA GGAGATGGAC TGGCTGGTGC TGGGGGAGCT GCAGATGGCC
    CTGGAGTGGG CAGGCAGGCC AGGGCTGCGC ATCAGTGGAC ACATTGCTGC CAGGAAGAGC
    TGCGACGTCC TGCAAAGTGT CCTTTGTGGG GCTGATGCCC TGATCCCAGT CCAGACGGGT
    GCTGCCGGCT CAGCCAGCCT CACGCTGCTA GGAAATGGCT CCCTGATCTA TCAGGTGCAA
    GTGGTAGGGA CAAGCAGTGA GGTGGTGGCC ATGACACTGG AGACCAAGCC TCAGCGGAGG
    GATCAGCGCA CTGTCCTGTG CCACATGGCT GGACTCCAGC CAGGAGGACA CACGGCCGTG
    GGTATCTGCC CTGGGCTGGG TGCCCGAGGG GCTCATATGC TGCTGCAGAA TGAGCTCTTC
    CTGAACGTGG GCACCAAGGA CTTCCCAGAC GGAGAGCTTC GGGGGCACGT GGCTGCCCTG
    CCCTACTGTG GGCATAGCGC CCGCCATGAC ACGCTGCCCG TGCCCCTAGC AGGAGCCCTG
    GTGCTACCCC CTGTGAAGAG CCAAGCAGCA GGGCACGCCT GGCTTTCCTT GGATACCCAC
    TGTCACCTGC ACTATGAAGT GCTGCTGGCT GGGCTTGGTG GCTCAGAACA AGGCACTGTC
    ACTGCCCACC TCCTTGGGCC TCCTGGAACG CCAGGGCCTC GGCGGCTGCT GAAGGGATTC
    TATGGCTCAG AGGCCCAGGG TGTGGTGAAG GACCTGGAGC CGGAACTGCT GCGGCACCTG
    GCAAAAGGCA TGGCCTCCCT GATGATCACC ACCAAGGGTA GCCCCAGAGG GGAGCTCCGA
    GGGCAGGTGC ACATAGCCAA CCAATGTGAG GTTGGCGGAC TGCGCCTGGA GGCGGCCGGG
    GCCGAGGGGG TGCGGGCGCT GGGGGCTCCG GATACAGCCT CTGCTGCGCC GCCTGTGGTG
    CCTGGTCTCC CGGCCCTAGC GCCCGCCAAA CCTGGTGGTC CTGGGCGGCC CCGAGACCCC
    AACACATGCT TCTTCGAGGG GCAGCAGCGC CCCCACGGGG CTCGCTGGGC GCCCAACTAC
    GACCCGCTCT GCTCACTCTG CACCTGCCAG AGACGAACGG TGATCTGTGA CCCGGTGGTG
    TGCCCACCGC CCAGCTGCCC ACACCCGGTG CAGGCTCCCG ACCAGTGCTG CCCTGTTTGC
    CCTGAGAAAC AAGATGTCAG AGACTTGCCA GGGCTGCCAA GGAGCCGGGA CCCAGGAGAG
    GGCTGCTATT TTGATGGTGA CCGGAGCTGG CGGGCAGCGG GTACGCGGTG GCACCCCGTT
    GTGCCCCCCT TTGGCTTAAT TAAGTGTGCT GTCTGCACCT GCAAGGGGGG CACTGGAGAG
    GTGCACTGTG AGAAGGTGCA GTGTCCCCGG CTGGCCTGTG CCCAGCCTGT GCGTGTCAAC
    CCCACCGACT GCTGCAAACA GTGTCCAGTG GGGTCGGGGG CCCACCCCCA GCTGGGGGAC
    CCCATGCAGG CTGATGGGCC CCGGGGCTGC CGTTTTGCTG GGCAGTGGTT CCCAGAGAGT
    CAGAGCTGGC ACCCCTCAGT GCCCCCTTTT GGAGAGATGA GCTGTATCAC CTGCAGATGT
    GGGGCAGGGG TGCCTCACTG TGAGCGGGAT GACTGTTCAC TGCCACTGTC CTGTGGCTCG
    GGGAAGGAGA GTCGATGCTG TTCCCGCTGC ACGGCCCACC GGCGGCCAGC CCCAGAGACC
    AGAACTGATC CAGAGCTGGA GAAAGAAGCC GAAGGCTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862883.1
    CDS935..2836
    Translation

    Target ORF information:

    RefSeq Version XM_017007394.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTGCGGCC TTGCAGGACT AACCCAGGTT CCCTTGAGGC TCCAGATTCT ACACCAGGGG 
    CAGCTACTGC GAGAACTTCA GGCCAATGTC TCAGCCCAGG AACCAGGCTT TGCTGAGGTG
    CTGCCCAACC TGACAGTCCA GGAGATGGAC TGGCTGGTGC TGGGGGAGCT GCAGATGGCC
    CTGGAGTGGG CAGGCAGGCC AGGGCTGCGC ATCAGTGGAC ACATTGCTGC CAGGAAGAGC
    TGCGACGTCC TGCAAAGTGT CCTTTGTGGG GCTGATGCCC TGATCCCAGT CCAGACGGGT
    GCTGCCGGCT CAGCCAGCCT CACGCTGCTA GGAAATGGCT CCCTGATCTA TCAGGTGCAA
    GTGGTAGGGA CAAGCAGTGA GGTGGTGGCC ATGACACTGG AGACCAAGCC TCAGCGGAGG
    GATCAGCGCA CTGTCCTGTG CCACATGGCT GGACTCCAGC CAGGAGGACA CACGGCCGTG
    GGTATCTGCC CTGGGCTGGG TGCCCGAGGG GCTCATATGC TGCTGCAGAA TGAGCTCTTC
    CTGAACGTGG GCACCAAGGA CTTCCCAGAC GGAGAGCTTC GGGGGCACGT GGCTGCCCTG
    CCCTACTGTG GGCATAGCGC CCGCCATGAC ACGCTGCCCG TGCCCCTAGC AGGAGCCCTG
    GTGCTACCCC CTGTGAAGAG CCAAGCAGCA GGGCACGCCT GGCTTTCCTT GGATACCCAC
    TGTCACCTGC ACTATGAAGT GCTGCTGGCT GGGCTTGGTG GCTCAGAACA AGGCACTGTC
    ACTGCCCACC TCCTTGGGCC TCCTGGAACG CCAGGGCCTC GGCGGCTGCT GAAGGGATTC
    TATGGCTCAG AGGCCCAGGG TGTGGTGAAG GACCTGGAGC CGGAACTGCT GCGGCACCTG
    GCAAAAGGCA TGGCCTCCCT GATGATCACC ACCAAGGGTA GCCCCAGAGG GGAGCTCCGA
    GGGCAGGTGC ACATAGCCAA CCAATGTGAG GTTGGCGGAC TGCGCCTGGA GGCGGCCGGG
    GCCGAGGGGG TGCGGGCGCT GGGGGCTCCG GATACAGCCT CTGCTGCGCC GCCTGTGGTG
    CCTGGTCTCC CGGCCCTAGC GCCCGCCAAA CCTGGTGGTC CTGGGCGGCC CCGAGACCCC
    AACACATGCT TCTTCGAGGG GCAGCAGCGC CCCCACGGGG CTCGCTGGGC GCCCAACTAC
    GACCCGCTCT GCTCACTCTG CACCTGCCAG AGACGAACGG TGATCTGTGA CCCGGTGGTG
    TGCCCACCGC CCAGCTGCCC ACACCCGGTG CAGGCTCCCG ACCAGTGCTG CCCTGTTTGC
    CCTGAGAAAC AAGATGTCAG AGACTTGCCA GGGCTGCCAA GGAGCCGGGA CCCAGGAGAG
    GGCTGCTATT TTGATGGTGA CCGGAGCTGG CGGGCAGCGG GTACGCGGTG GCACCCCGTT
    GTGCCCCCCT TTGGCTTAAT TAAGTGTGCT GTCTGCACCT GCAAGGGGGG CACTGGAGAG
    GTGCACTGTG AGAAGGTGCA GTGTCCCCGG CTGGCCTGTG CCCAGCCTGT GCGTGTCAAC
    CCCACCGACT GCTGCAAACA GTGTCCAGTG GGGTCGGGGG CCCACCCCCA GCTGGGGGAC
    CCCATGCAGG CTGATGGGCC CCGGGGCTGC CGTTTTGCTG GGCAGTGGTT CCCAGAGAGT
    CAGAGCTGGC ACCCCTCAGT GCCCCCTTTT GGAGAGATGA GCTGTATCAC CTGCAGATGT
    GGGGCAGGGG TGCCTCACTG TGAGCGGGAT GACTGTTCAC TGCCACTGTC CTGTGGCTCG
    GGGAAGGAGA GTCGATGCTG TTCCCGCTGC ACGGCCCACC GGCGGCCAGC CCCAGAGACC
    AGAACTGATC CAGAGCTGGA GAAAGAAGCC GAAGGCTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38216
    Clone ID Related Accession (Same CDS sequence) NM_001304472.1
    Accession Version NM_001304472.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Homo sapiens(human)
    Product chordin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF209928.1, BC112345.1, AY358926.2 and BC029879.1. On Jan 28, 2015 this sequence version replaced XM_005247850.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1 The encoded isoform (2) is one amino acid shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358926.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGGTGCACAT AGCCAACCAA TGTGAGGTTG GCGGACTGCG CCTGGAGGCG
    GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG GCTCCGGATA CAGCCTCTGC TGCGCCGCCT
    GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC GCCAAACCTG GTGGTCCTGG GCGGCCCCGA
    GACCCCAACA CATGCTTCTT CGAGGGGCAG CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC
    AACTACGACC CGCTCTGCTC ACTCTGCACC TGCCAGAGAC GAACGGTGAT CTGTGACCCG
    GTGGTGTGCC CACCGCCCAG CTGCCCACAC CCGGTGCAGG CTCCCGACCA GTGCTGCCCT
    GTTTGCCCTG AGAAACAAGA TGTCAGAGAC TTGCCAGGGC TGCCAAGGAG CCGGGACCCA
    GGAGAGGGCT GCTATTTTGA TGGTGACCGG AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC
    CCCGTTGTGC CCCCCTTTGG CTTAATTAAG TGTGCTGTCT GCACCTGCAA GGGGGGCACT
    GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT
    GTCAACCCCA CCGACTGCTG CAAACAGTGT CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG
    GGGGACCCCA TGCAGGCTGA TGGGCCCCGG GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA
    GAGAGTCAGA GCTGGCACCC CTCAGTGCCC CCTTTTGGAG AGATGAGCTG TATCACCTGC
    AGATGTGGGG CAGGGGTGCC TCACTGTGAG CGGGATGACT GTTCACTGCC ACTGTCCTGT
    GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC CGCTGCACGG CCCACCGGCG GCCCCCAGAG
    ACCAGAACTG ATCCAGAGCT GGAGAAAGAA GCCGAAGGCT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291401.1
    CDS247..3111
    Translation

    Target ORF information:

    RefSeq Version NM_001304472.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304472.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGGTGCACAT AGCCAACCAA TGTGAGGTTG GCGGACTGCG CCTGGAGGCG
    GCCGGGGCCG AGGGGGTGCG GGCGCTGGGG GCTCCGGATA CAGCCTCTGC TGCGCCGCCT
    GTGGTGCCTG GTCTCCCGGC CCTAGCGCCC GCCAAACCTG GTGGTCCTGG GCGGCCCCGA
    GACCCCAACA CATGCTTCTT CGAGGGGCAG CAGCGCCCCC ACGGGGCTCG CTGGGCGCCC
    AACTACGACC CGCTCTGCTC ACTCTGCACC TGCCAGAGAC GAACGGTGAT CTGTGACCCG
    GTGGTGTGCC CACCGCCCAG CTGCCCACAC CCGGTGCAGG CTCCCGACCA GTGCTGCCCT
    GTTTGCCCTG AGAAACAAGA TGTCAGAGAC TTGCCAGGGC TGCCAAGGAG CCGGGACCCA
    GGAGAGGGCT GCTATTTTGA TGGTGACCGG AGCTGGCGGG CAGCGGGTAC GCGGTGGCAC
    CCCGTTGTGC CCCCCTTTGG CTTAATTAAG TGTGCTGTCT GCACCTGCAA GGGGGGCACT
    GGAGAGGTGC ACTGTGAGAA GGTGCAGTGT CCCCGGCTGG CCTGTGCCCA GCCTGTGCGT
    GTCAACCCCA CCGACTGCTG CAAACAGTGT CCAGTGGGGT CGGGGGCCCA CCCCCAGCTG
    GGGGACCCCA TGCAGGCTGA TGGGCCCCGG GGCTGCCGTT TTGCTGGGCA GTGGTTCCCA
    GAGAGTCAGA GCTGGCACCC CTCAGTGCCC CCTTTTGGAG AGATGAGCTG TATCACCTGC
    AGATGTGGGG CAGGGGTGCC TCACTGTGAG CGGGATGACT GTTCACTGCC ACTGTCCTGT
    GGCTCGGGGA AGGAGAGTCG ATGCTGTTCC CGCTGCACGG CCCACCGGCG GCCCCCAGAG
    ACCAGAACTG ATCCAGAGCT GGAGAAAGAA GCCGAAGGCT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62090
    Clone ID Related Accession (Same CDS sequence) XM_011513254.1
    Accession Version XM_011513254.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2604bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGAGACGAAC GGTGATCTGT GACCCGGTGG TGTGCCCACC GCCCAGCTGC
    CCACACCCGG TGCAGGCTCC CGACCAGTGC TGCCCTGTTT GCCCTGAGAA ACAAGATGTC
    AGAGACTTGC CAGGGCTGCC AAGGAGCCGG GACCCAGGAG AGGGCTGCTA TTTTGATGGT
    GACCGGAGCT GGCGGGCAGC GGGTACGCGG TGGCACCCCG TTGTGCCCCC CTTTGGCTTA
    ATTAAGTGTG CTGTCTGCAC CTGCAAGGGG GGCACTGGAG AGGTGCACTG TGAGAAGGTG
    CAGTGTCCCC GGCTGGCCTG TGCCCAGCCT GTGCGTGTCA ACCCCACCGA CTGCTGCAAA
    CAGTGTCCAG TGGGGTCGGG GGCCCACCCC CAGCTGGGGG ACCCCATGCA GGCTGATGGG
    CCCCGGGGCT GCCGTTTTGC TGGGCAGTGG TTCCCAGAGA GTCAGAGCTG GCACCCCTCA
    GTGCCCCCTT TTGGAGAGAT GAGCTGTATC ACCTGCAGAT GTGGGGCAGG GGTGCCTCAC
    TGTGAGCGGG ATGACTGTTC ACTGCCACTG TCCTGTGGCT CGGGGAAGGA GAGTCGATGC
    TGTTCCCGCT GCACGGCCCA CCGGCGGCCA GCCCCAGAGA CCAGAACTGA TCCAGAGCTG
    GAGAAAGAAG CCGAAGGCTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511556.1
    CDS100..2703
    Translation

    Target ORF information:

    RefSeq Version XM_011513254.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513254.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGAGACGAAC GGTGATCTGT GACCCGGTGG TGTGCCCACC GCCCAGCTGC
    CCACACCCGG TGCAGGCTCC CGACCAGTGC TGCCCTGTTT GCCCTGAGAA ACAAGATGTC
    AGAGACTTGC CAGGGCTGCC AAGGAGCCGG GACCCAGGAG AGGGCTGCTA TTTTGATGGT
    GACCGGAGCT GGCGGGCAGC GGGTACGCGG TGGCACCCCG TTGTGCCCCC CTTTGGCTTA
    ATTAAGTGTG CTGTCTGCAC CTGCAAGGGG GGCACTGGAG AGGTGCACTG TGAGAAGGTG
    CAGTGTCCCC GGCTGGCCTG TGCCCAGCCT GTGCGTGTCA ACCCCACCGA CTGCTGCAAA
    CAGTGTCCAG TGGGGTCGGG GGCCCACCCC CAGCTGGGGG ACCCCATGCA GGCTGATGGG
    CCCCGGGGCT GCCGTTTTGC TGGGCAGTGG TTCCCAGAGA GTCAGAGCTG GCACCCCTCA
    GTGCCCCCTT TTGGAGAGAT GAGCTGTATC ACCTGCAGAT GTGGGGCAGG GGTGCCTCAC
    TGTGAGCGGG ATGACTGTTC ACTGCCACTG TCCTGTGGCT CGGGGAAGGA GAGTCGATGC
    TGTTCCCGCT GCACGGCCCA CCGGCGGCCA GCCCCAGAGA CCAGAACTGA TCCAGAGCTG
    GAGAAAGAAG CCGAAGGCTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94712
    Clone ID Related Accession (Same CDS sequence) XM_017007388.1
    Accession Version XM_017007388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGGTGCACA TAGCCAACCA ATGTGAGGTT
    GGCGGACTGC GCCTGGAGGC GGCCGGGGCC GAGGGGGTGC GGGCGCTGGG GGCTCCGGAT
    ACAGCCTCTG CTGCGCCGCC TGTGGTGCCT GGTCTCCCGG CCCTAGCGCC CGCCAAACCT
    GGTGGTCCTG GGCGGCCCCG AGACCCCAAC ACATGCTTCT TCGAGGGGCA GCAGCGCCCC
    CACGGGGCTC GCTGGGCGCC CAACTACGAC CCGCTCTGCT CACTCTGCAC CTGCCAGAGA
    CGAACGGTGA TCTGTGACCC GGTGGTGTGC CCACCGCCCA GCTGCCCACA CCCGGTGCAG
    GCTCCCGACC AGTGCTGCCC TGTTTGCCCT GAGAAACAAG ATGTCAGAGA CTTGCCAGGG
    CTGCCAAGGA GCCGGGACCC AGGAGAGGGC TGCTATTTTG ATGGTGACCG GAGCTGGCGG
    GCAGCGGGTA CGCGGTGGCA CCCCGTTGTG CCCCCCTTTG GCTTAATTAA GTGTGCTGTC
    TGCACCTGCA AGGGGGGCAC TGGAGAGGTG CACTGTGAGA AGGTGCAGTG TCCCCGGCTG
    GCCTGTGCCC AGCCTGTGCG TGTCAACCCC ACCGACTGCT GCAAACAGTG TCCAGTGGGG
    TCGGGGGCCC ACCCCCAGCT GGGGGACCCC ATGCAGGCTG ATGGGCCCCG GGGCTGCCGT
    TTTGCTGGGC AGTGGTTCCC AGAGAGTCAG AGCTGGCACC CCTCAGTGCC CCCTTTTGGA
    GAGATGAGCT GTATCACCTG CAGATGTGGG GCAGGGGTGC CTCACTGTGA GCGGGATGAC
    TGTTCACTGC CACTGTCCTG TGGCTCGGGG AAGGAGAGTC GATGCTGTTC CCGCTGCACG
    GCCCACCGGC GGCCAGCCCC AGAGACCAGA ACTGATCCAG AGCTGGAGAA AGAAGCCGAA
    GGCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862877.1
    CDS100..3048
    Translation

    Target ORF information:

    RefSeq Version XM_017007388.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGGTGCACA TAGCCAACCA ATGTGAGGTT
    GGCGGACTGC GCCTGGAGGC GGCCGGGGCC GAGGGGGTGC GGGCGCTGGG GGCTCCGGAT
    ACAGCCTCTG CTGCGCCGCC TGTGGTGCCT GGTCTCCCGG CCCTAGCGCC CGCCAAACCT
    GGTGGTCCTG GGCGGCCCCG AGACCCCAAC ACATGCTTCT TCGAGGGGCA GCAGCGCCCC
    CACGGGGCTC GCTGGGCGCC CAACTACGAC CCGCTCTGCT CACTCTGCAC CTGCCAGAGA
    CGAACGGTGA TCTGTGACCC GGTGGTGTGC CCACCGCCCA GCTGCCCACA CCCGGTGCAG
    GCTCCCGACC AGTGCTGCCC TGTTTGCCCT GAGAAACAAG ATGTCAGAGA CTTGCCAGGG
    CTGCCAAGGA GCCGGGACCC AGGAGAGGGC TGCTATTTTG ATGGTGACCG GAGCTGGCGG
    GCAGCGGGTA CGCGGTGGCA CCCCGTTGTG CCCCCCTTTG GCTTAATTAA GTGTGCTGTC
    TGCACCTGCA AGGGGGGCAC TGGAGAGGTG CACTGTGAGA AGGTGCAGTG TCCCCGGCTG
    GCCTGTGCCC AGCCTGTGCG TGTCAACCCC ACCGACTGCT GCAAACAGTG TCCAGTGGGG
    TCGGGGGCCC ACCCCCAGCT GGGGGACCCC ATGCAGGCTG ATGGGCCCCG GGGCTGCCGT
    TTTGCTGGGC AGTGGTTCCC AGAGAGTCAG AGCTGGCACC CCTCAGTGCC CCCTTTTGGA
    GAGATGAGCT GTATCACCTG CAGATGTGGG GCAGGGGTGC CTCACTGTGA GCGGGATGAC
    TGTTCACTGC CACTGTCCTG TGGCTCGGGG AAGGAGAGTC GATGCTGTTC CCGCTGCACG
    GCCCACCGGC GGCCAGCCCC AGAGACCAGA ACTGATCCAG AGCTGGAGAA AGAAGCCGAA
    GGCTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94713
    Clone ID Related Accession (Same CDS sequence) XM_017007389.1
    Accession Version XM_017007389.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2946bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGGTGCACA TAGCCAACCA ATGTGAGGTT
    GGCGGACTGC GCCTGGAGGC GGCCGGGGCC GAGGGGGTGC GGGCGCTGGG GGCTCCGGAT
    ACAGCCTCTG CTGCGCCGCC TGTGGTGCCT GGTCTCCCGG CCCTAGCGCC CGCCAAACCT
    GGTGGTCCTG GGCGGCCCCG AGACCCCAAC ACATGCTTCT TCGAGGGGCA GCAGCGCCCC
    CACGGGGCTC GCTGGGCGCC CAACTACGAC CCGCTCTGCT CACTCTGCAC CTGCCAGAGA
    CGAACGGTGA TCTGTGACCC GGTGGTGTGC CCACCGCCCA GCTGCCCACA CCCGGTGCAG
    GCTCCCGACC AGTGCTGCCC TGTTTGCCCT GAGAAACAAG ATGTCAGAGA CTTGCCAGGG
    CTGCCAAGGA GCCGGGACCC AGGAGAGGGC TGCTATTTTG ATGGTGACCG GAGCTGGCGG
    GCAGCGGGTA CGCGGTGGCA CCCCGTTGTG CCCCCCTTTG GCTTAATTAA GTGTGCTGTC
    TGCACCTGCA AGGGGGGCAC TGGAGAGGTG CACTGTGAGA AGGTGCAGTG TCCCCGGCTG
    GCCTGTGCCC AGCCTGTGCG TGTCAACCCC ACCGACTGCT GCAAACAGTG TCCAGTGGGG
    TCGGGGGCCC ACCCCCAGCT GGGGGACCCC ATGCAGGCTG ATGGGCCCCG GGGCTGCCGT
    TTTGCTGGGC AGTGGTTCCC AGAGAGTCAG AGCTGGCACC CCTCAGTGCC CCCTTTTGGA
    GAGATGAGCT GTATCACCTG CAGATGTGGG GCAGGGGTGC CTCACTGTGA GCGGGATGAC
    TGTTCACTGC CACTGTCCTG TGGCTCGGGG AAGGAGAGTC GATGCTGTTC CCGCTGCACG
    GCCCACCGGC GGCCCCCAGA GACCAGAACT GATCCAGAGC TGGAGAAAGA AGCCGAAGGC
    TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862878.1
    CDS100..3045
    Translation

    Target ORF information:

    RefSeq Version XM_017007389.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007389.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGGTGCACA TAGCCAACCA ATGTGAGGTT
    GGCGGACTGC GCCTGGAGGC GGCCGGGGCC GAGGGGGTGC GGGCGCTGGG GGCTCCGGAT
    ACAGCCTCTG CTGCGCCGCC TGTGGTGCCT GGTCTCCCGG CCCTAGCGCC CGCCAAACCT
    GGTGGTCCTG GGCGGCCCCG AGACCCCAAC ACATGCTTCT TCGAGGGGCA GCAGCGCCCC
    CACGGGGCTC GCTGGGCGCC CAACTACGAC CCGCTCTGCT CACTCTGCAC CTGCCAGAGA
    CGAACGGTGA TCTGTGACCC GGTGGTGTGC CCACCGCCCA GCTGCCCACA CCCGGTGCAG
    GCTCCCGACC AGTGCTGCCC TGTTTGCCCT GAGAAACAAG ATGTCAGAGA CTTGCCAGGG
    CTGCCAAGGA GCCGGGACCC AGGAGAGGGC TGCTATTTTG ATGGTGACCG GAGCTGGCGG
    GCAGCGGGTA CGCGGTGGCA CCCCGTTGTG CCCCCCTTTG GCTTAATTAA GTGTGCTGTC
    TGCACCTGCA AGGGGGGCAC TGGAGAGGTG CACTGTGAGA AGGTGCAGTG TCCCCGGCTG
    GCCTGTGCCC AGCCTGTGCG TGTCAACCCC ACCGACTGCT GCAAACAGTG TCCAGTGGGG
    TCGGGGGCCC ACCCCCAGCT GGGGGACCCC ATGCAGGCTG ATGGGCCCCG GGGCTGCCGT
    TTTGCTGGGC AGTGGTTCCC AGAGAGTCAG AGCTGGCACC CCTCAGTGCC CCCTTTTGGA
    GAGATGAGCT GTATCACCTG CAGATGTGGG GCAGGGGTGC CTCACTGTGA GCGGGATGAC
    TGTTCACTGC CACTGTCCTG TGGCTCGGGG AAGGAGAGTC GATGCTGTTC CCGCTGCACG
    GCCCACCGGC GGCCCCCAGA GACCAGAACT GATCCAGAGC TGGAGAAAGA AGCCGAAGGC
    TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94714
    Clone ID Related Accession (Same CDS sequence) XM_017007390.1
    Accession Version XM_017007390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2685bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGAGACGAA CGGTGATCTG TGACCCGGTG
    GTGTGCCCAC CGCCCAGCTG CCCACACCCG GTGCAGGCTC CCGACCAGTG CTGCCCTGTT
    TGCCCTGAGA AACAAGATGT CAGAGACTTG CCAGGGCTGC CAAGGAGCCG GGACCCAGGA
    GAGGGCTGCT ATTTTGATGG TGACCGGAGC TGGCGGGCAG CGGGTACGCG GTGGCACCCC
    GTTGTGCCCC CCTTTGGCTT AATTAAGTGT GCTGTCTGCA CCTGCAAGGG GGGCACTGGA
    GAGGTGCACT GTGAGAAGGT GCAGTGTCCC CGGCTGGCCT GTGCCCAGCC TGTGCGTGTC
    AACCCCACCG ACTGCTGCAA ACAGTGTCCA GTGGGGTCGG GGGCCCACCC CCAGCTGGGG
    GACCCCATGC AGGCTGATGG GCCCCGGGGC TGCCGTTTTG CTGGGCAGTG GTTCCCAGAG
    AGTCAGAGCT GGCACCCCTC AGTGCCCCCT TTTGGAGAGA TGAGCTGTAT CACCTGCAGA
    TGTGGGGCAG GGGTGCCTCA CTGTGAGCGG GATGACTGTT CACTGCCACT GTCCTGTGGC
    TCGGGGAAGG AGAGTCGATG CTGTTCCCGC TGCACGGCCC ACCGGCGGCC AGCCCCAGAG
    ACCAGAACTG ATCCAGAGCT GGAGAAAGAA GCCGAAGGCT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862879.1
    CDS100..2784
    Translation

    Target ORF information:

    RefSeq Version XM_017007390.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGAGACGAA CGGTGATCTG TGACCCGGTG
    GTGTGCCCAC CGCCCAGCTG CCCACACCCG GTGCAGGCTC CCGACCAGTG CTGCCCTGTT
    TGCCCTGAGA AACAAGATGT CAGAGACTTG CCAGGGCTGC CAAGGAGCCG GGACCCAGGA
    GAGGGCTGCT ATTTTGATGG TGACCGGAGC TGGCGGGCAG CGGGTACGCG GTGGCACCCC
    GTTGTGCCCC CCTTTGGCTT AATTAAGTGT GCTGTCTGCA CCTGCAAGGG GGGCACTGGA
    GAGGTGCACT GTGAGAAGGT GCAGTGTCCC CGGCTGGCCT GTGCCCAGCC TGTGCGTGTC
    AACCCCACCG ACTGCTGCAA ACAGTGTCCA GTGGGGTCGG GGGCCCACCC CCAGCTGGGG
    GACCCCATGC AGGCTGATGG GCCCCGGGGC TGCCGTTTTG CTGGGCAGTG GTTCCCAGAG
    AGTCAGAGCT GGCACCCCTC AGTGCCCCCT TTTGGAGAGA TGAGCTGTAT CACCTGCAGA
    TGTGGGGCAG GGGTGCCTCA CTGTGAGCGG GATGACTGTT CACTGCCACT GTCCTGTGGC
    TCGGGGAAGG AGAGTCGATG CTGTTCCCGC TGCACGGCCC ACCGGCGGCC AGCCCCAGAG
    ACCAGAACTG ATCCAGAGCT GGAGAAAGAA GCCGAAGGCT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94715
    Clone ID Related Accession (Same CDS sequence) XM_017007391.1
    Accession Version XM_017007391.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGAGACGAA CGGTGATCTG TGACCCGGTG
    GTGTGCCCAC CGCCCAGCTG CCCACACCCG GTGCAGGCTC CCGACCAGTG CTGCCCTGTT
    TGCCCTGAGA AACAAGATGT CAGAGACTTG CCAGGGCTGC CAAGGAGCCG GGACCCAGGA
    GAGGGCTGCT ATTTTGATGG TGACCGGAGC TGGCGGGCAG CGGGTACGCG GTGGCACCCC
    GTTGTGCCCC CCTTTGGCTT AATTAAGTGT GCTGTCTGCA CCTGCAAGGG GGGCACTGGA
    GAGGTGCACT GTGAGAAGGT GCAGTGTCCC CGGCTGGCCT GTGCCCAGCC TGTGCGTGTC
    AACCCCACCG ACTGCTGCAA ACAGTGTCCA GTGGGGTCGG GGGCCCACCC CCAGCTGGGG
    GACCCCATGC AGGCTGATGG GCCCCGGGGC TGCCGTTTTG CTGGGCAGTG GTTCCCAGAG
    AGTCAGAGCT GGCACCCCTC AGTGCCCCCT TTTGGAGAGA TGAGCTGTAT CACCTGCAGA
    TGTGGGGCAG GGGTGCCTCA CTGTGAGCGG GATGACTGTT CACTGCCACT GTCCTGTGGC
    TCGGGGAAGG AGAGTCGATG CTGTTCCCGC TGCACGGCCC ACCGGCGGCC CCCAGAGACC
    AGAACTGATC CAGAGCTGGA GAAAGAAGCC GAAGGCTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862880.1
    CDS100..2781
    Translation

    Target ORF information:

    RefSeq Version XM_017007391.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007391.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGAGACGAA CGGTGATCTG TGACCCGGTG
    GTGTGCCCAC CGCCCAGCTG CCCACACCCG GTGCAGGCTC CCGACCAGTG CTGCCCTGTT
    TGCCCTGAGA AACAAGATGT CAGAGACTTG CCAGGGCTGC CAAGGAGCCG GGACCCAGGA
    GAGGGCTGCT ATTTTGATGG TGACCGGAGC TGGCGGGCAG CGGGTACGCG GTGGCACCCC
    GTTGTGCCCC CCTTTGGCTT AATTAAGTGT GCTGTCTGCA CCTGCAAGGG GGGCACTGGA
    GAGGTGCACT GTGAGAAGGT GCAGTGTCCC CGGCTGGCCT GTGCCCAGCC TGTGCGTGTC
    AACCCCACCG ACTGCTGCAA ACAGTGTCCA GTGGGGTCGG GGGCCCACCC CCAGCTGGGG
    GACCCCATGC AGGCTGATGG GCCCCGGGGC TGCCGTTTTG CTGGGCAGTG GTTCCCAGAG
    AGTCAGAGCT GGCACCCCTC AGTGCCCCCT TTTGGAGAGA TGAGCTGTAT CACCTGCAGA
    TGTGGGGCAG GGGTGCCTCA CTGTGAGCGG GATGACTGTT CACTGCCACT GTCCTGTGGC
    TCGGGGAAGG AGAGTCGATG CTGTTCCCGC TGCACGGCCC ACCGGCGGCC CCCAGAGACC
    AGAACTGATC CAGAGCTGGA GAAAGAAGCC GAAGGCTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94716
    Clone ID Related Accession (Same CDS sequence) XM_017007392.1
    Accession Version XM_017007392.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGAGACGAAC GGTGATCTGT GACCCGGTGG TGTGCCCACC GCCCAGCTGC
    CCACACCCGG TGCAGGCTCC CGACCAGTGC TGCCCTGTTT GCCCTGAGAA ACAAGATGTC
    AGAGACTTGC CAGGGCTGCC AAGGAGCCGG GACCCAGGAG AGGGCTGCTA TTTTGATGGT
    GACCGGAGCT GGCGGGCAGC GGGTACGCGG TGGCACCCCG TTGTGCCCCC CTTTGGCTTA
    ATTAAGTGTG CTGTCTGCAC CTGCAAGGGG GGCACTGGAG AGGTGCACTG TGAGAAGGTG
    CAGTGTCCCC GGCTGGCCTG TGCCCAGCCT GTGCGTGTCA ACCCCACCGA CTGCTGCAAA
    CAGTGTCCAG TGGGGTCGGG GGCCCACCCC CAGCTGGGGG ACCCCATGCA GGCTGATGGG
    CCCCGGGGCT GCCGTTTTGC TGGGCAGTGG TTCCCAGAGA GTCAGAGCTG GCACCCCTCA
    GTGCCCCCTT TTGGAGAGAT GAGCTGTATC ACCTGCAGAT GTGGGGCAGG GGTGCCTCAC
    TGTGAGCGGG ATGACTGTTC ACTGCCACTG TCCTGTGGCT CGGGGAAGGA GAGTCGATGC
    TGTTCCCGCT GCACGGCCCA CCGGCGGCCC CCAGAGACCA GAACTGATCC AGAGCTGGAG
    AAAGAAGCCG AAGGCTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862881.1
    CDS100..2700
    Translation

    Target ORF information:

    RefSeq Version XM_017007392.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007392.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGACTAACC CAGGTTCCCT TGAGGCTCCA GATTCTACAC
    CAGGGGCAGC TACTGCGAGA ACTTCAGGCC AATGTCTCAG CCCAGGAACC AGGCTTTGCT
    GAGGTGCTGC CCAACCTGAC AGTCCAGGAG ATGGACTGGC TGGTGCTGGG GGAGCTGCAG
    ATGGCCCTGG AGTGGGCAGG CAGGCCAGGG CTGCGCATCA GTGGACACAT TGCTGCCAGG
    AAGAGCTGCG ACGTCCTGCA AAGTGTCCTT TGTGGGGCTG ATGCCCTGAT CCCAGTCCAG
    ACGGGTGCTG CCGGCTCAGC CAGCCTCACG CTGCTAGGAA ATGGCTCCCT GATCTATCAG
    GTGCAAGTGG TAGGGACAAG CAGTGAGGTG GTGGCCATGA CACTGGAGAC CAAGCCTCAG
    CGGAGGGATC AGCGCACTGT CCTGTGCCAC ATGGCTGGAC TCCAGCCAGG AGGACACACG
    GCCGTGGGTA TCTGCCCTGG GCTGGGTGCC CGAGGGGCTC ATATGCTGCT GCAGAATGAG
    CTCTTCCTGA ACGTGGGCAC CAAGGACTTC CCAGACGGAG AGCTTCGGGG GCACGTGGCT
    GCCCTGCCCT ACTGTGGGCA TAGCGCCCGC CATGACACGC TGCCCGTGCC CCTAGCAGGA
    GCCCTGGTGC TACCCCCTGT GAAGAGCCAA GCAGCAGGGC ACGCCTGGCT TTCCTTGGAT
    ACCCACTGTC ACCTGCACTA TGAAGTGCTG CTGGCTGGGC TTGGTGGCTC AGAACAAGGC
    ACTGTCACTG CCCACCTCCT TGGGCCTCCT GGAACGCCAG GGCCTCGGCG GCTGCTGAAG
    GGATTCTATG GCTCAGAGGC CCAGGGTGTG GTGAAGGACC TGGAGCCGGA ACTGCTGCGG
    CACCTGGCAA AAGGCATGGC CTCCCTGATG ATCACCACCA AGGGTAGCCC CAGAGGGGAG
    CTCCGAGGGC AGAGACGAAC GGTGATCTGT GACCCGGTGG TGTGCCCACC GCCCAGCTGC
    CCACACCCGG TGCAGGCTCC CGACCAGTGC TGCCCTGTTT GCCCTGAGAA ACAAGATGTC
    AGAGACTTGC CAGGGCTGCC AAGGAGCCGG GACCCAGGAG AGGGCTGCTA TTTTGATGGT
    GACCGGAGCT GGCGGGCAGC GGGTACGCGG TGGCACCCCG TTGTGCCCCC CTTTGGCTTA
    ATTAAGTGTG CTGTCTGCAC CTGCAAGGGG GGCACTGGAG AGGTGCACTG TGAGAAGGTG
    CAGTGTCCCC GGCTGGCCTG TGCCCAGCCT GTGCGTGTCA ACCCCACCGA CTGCTGCAAA
    CAGTGTCCAG TGGGGTCGGG GGCCCACCCC CAGCTGGGGG ACCCCATGCA GGCTGATGGG
    CCCCGGGGCT GCCGTTTTGC TGGGCAGTGG TTCCCAGAGA GTCAGAGCTG GCACCCCTCA
    GTGCCCCCTT TTGGAGAGAT GAGCTGTATC ACCTGCAGAT GTGGGGCAGG GGTGCCTCAC
    TGTGAGCGGG ATGACTGTTC ACTGCCACTG TCCTGTGGCT CGGGGAAGGA GAGTCGATGC
    TGTTCCCGCT GCACGGCCCA CCGGCGGCCC CCAGAGACCA GAACTGATCC AGAGCTGGAG
    AAAGAAGCCG AAGGCTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu94717
    Clone ID Related Accession (Same CDS sequence) XM_017007393.1
    Accession Version XM_017007393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product chordin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGCGCCCCC CCGGCTGGTG GGGAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862882.1
    CDS100..2139
    Translation

    Target ORF information:

    RefSeq Version XM_017007393.1
    Organism Homo sapiens(human)
    Definition Homo sapiens chordin (CHRD), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCGAGCC TCCCGGCCCC GCCGGCCCCG CTGCTGCTCC TCGGGCTGCT GCTGCTCGGC 
    TCCCGGCCGG CCCGCGGCGC CGGCCCAGAG CCCCCCGTGC TGCCCATCCG TTCTGAGAAG
    GAGCCGCTGC CCGTTCGGGG AGCGGCAGGC TGCACCTTCG GCGGGAAGGT CTATGCCTTG
    GACGAGACGT GGCACCCGGA CCTAGGGGAG CCATTCGGGG TGATGCGCTG CGTGCTGTGC
    GCCTGCGAGG CGCCTCAGTG GGGTCGCCGT ACCAGGGGCC CTGGCAGGGT CAGCTGCAAG
    AACATCAAAC CAGAGTGCCC AACCCCGGCC TGTGGGCAGC CGCGCCAGCT GCCGGGACAC
    TGCTGCCAGA CCTGCCCCCA GGAGCGCAGC AGTTCGGAGC GGCAGCCGAG CGGCCTGTCC
    TTCGAGTATC CGCGGGACCC GGAGCATCGC AGTTATAGCG ACCGCGGGGA GCCAGGCGCT
    GAGGAGCGGG CCCGTGGTGA CGGCCACACG GACTTCGTGG CGCTGCTGAC AGGGCCGAGG
    TCGCAGGCGG TGGCACGAGC CCGAGTCTCG CTGCTGCGCT CTAGCCTCCG CTTCTCTATC
    TCCTACAGGC GGCTGGACCG CCCTACCAGG ATCCGCTTCT CAGACTCCAA TGGCAGTGTC
    CTGTTTGAGC ACCCTGCAGC CCCCACCCAA GATGGCCTGG TCTGTGGGGT GTGGCGGGCA
    GTGCCTCGGT TGTCTCTGCG GCTCCTTAGG GCAGAACAGC TGCATGTGGC ACTTGTGACA
    CTCACTCACC CTTCAGGGGA GGTCTGGGGG CCTCTCATCC GGCACCGGGC CCTGGCTGCA
    GAGACCTTCA GTGCCATCCT GACTCTAGAA GGCCCCCCAC AGCAGGGCGT AGGGGGCATC
    ACCCTGCTCA CTCTCAGTGA CACAGAGGAC TCCTTGCATT TTTTGCTGCT CTTCCGAGGG
    CTGCTGGAAC CCAGGAGTGG GGGTAAGTGG GATGGGGGCA AAACACGTGA GAAGGTTAGG
    GAGAGCACCT GTCTCAGAAA GGCCCACATG TGCGGCCTTG CAGGACTAAC CCAGGTTCCC
    TTGAGGCTCC AGATTCTACA CCAGGGGCAG CTACTGCGAG AACTTCAGGC CAATGTCTCA
    GCCCAGGAAC CAGGCTTTGC TGAGGTGCTG CCCAACCTGA CAGTCCAGGA GATGGACTGG
    CTGGTGCTGG GGGAGCTGCA GATGGCCCTG GAGTGGGCAG GCAGGCCAGG GCTGCGCATC
    AGTGGACACA TTGCTGCCAG GAAGAGCTGC GACGTCCTGC AAAGTGTCCT TTGTGGGGCT
    GATGCCCTGA TCCCAGTCCA GACGGGTGCT GCCGGCTCAG CCAGCCTCAC GCTGCTAGGA
    AATGGCTCCC TGATCTATCA GGTGCAAGTG GTAGGGACAA GCAGTGAGGT GGTGGCCATG
    ACACTGGAGA CCAAGCCTCA GCGGAGGGAT CAGCGCACTG TCCTGTGCCA CATGGCTGGA
    CTCCAGCCAG GAGGACACAC GGCCGTGGGT ATCTGCCCTG GGCTGGGTGC CCGAGGGGCT
    CATATGCTGC TGCAGAATGA GCTCTTCCTG AACGTGGGCA CCAAGGACTT CCCAGACGGA
    GAGCTTCGGG GGCACGTGGC TGCCCTGCCC TACTGTGGGC ATAGCGCCCG CCATGACACG
    CTGCCCGTGC CCCTAGCAGG AGCCCTGGTG CTACCCCCTG TGAAGAGCCA AGCAGCAGGG
    CACGCCTGGC TTTCCTTGGA TACCCACTGT CACCTGCACT ATGAAGTGCT GCTGGCTGGG
    CTTGGTGGCT CAGAACAAGG CACTGTCACT GCCCACCTCC TTGGGCCTCC TGGAACGCCA
    GGGCCTCGGC GGCTGCTGAA GGGATTCTAT GGCTCAGAGG CCCAGGGTGT GGTGAAGGAC
    CTGGAGCCGG AACTGCTGCG GCACCTGGCA AAAGGCATGG CCTCCCTGAT GATCACCACC
    AAGGGTAGCC CCAGAGGGGA GCTCCGAGGG CAGCGCCCCC CCGGCTGGTG GGGAGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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