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EDF1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol EDF1
Entrez Gene ID 8721
Full Name endothelial differentiation related factor 1
Synonyms CFAP280,EDF-1,MBF1
General protein information
Preferred Names

endothelial differentiation related factor 1

Names

endothelial differentiation-related factor 1
multiprotein bridging factor 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

9

9q34.3
Summary This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
Disorder MIM:

605107


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001281297.1 NP_001268226.1 endothelial differentiation-related factor 1 isoform 3
NM_001281298.2 NP_001268227.1 endothelial differentiation-related factor 1 isoform 4
NM_001281297.2 NP_001268226.1 endothelial differentiation-related factor 1 isoform 3
NM_003792.3 NP_003783.1 endothelial differentiation-related factor 1 isoform alpha
NM_001281299.1 NP_001268228.1 endothelial differentiation-related factor 1 isoform 5
NM_153200.2 NP_694880.1 endothelial differentiation-related factor 1 isoform beta
NM_001281298.1 NP_001268227.1 endothelial differentiation-related factor 1 isoform 4
NM_003792.4 NP_003783.1 endothelial differentiation-related factor 1 isoform alpha
NM_001281299.2 NP_001268228.1 endothelial differentiation-related factor 1 isoform 5

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) EDF1 XP_537793.4
Pan troglodytes (chimpanzee) EDF1 XP_001163031.2
Gallus gallus (chicken) EDF1 NP_001006203.1
Schizosaccharomyces pombe (fission yeast) SPBC83.17 NP_595650.1
Oryza sativa (rice) Os08g0366100 NP_001061647.1
Xenopus tropicalis (tropical clawed frog) edf1 NP_001016686.1
Homo sapiens (human) EDF1 NP_003783.1
Bos taurus (cattle) EDF1 NP_001030384.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004990 XP_315094.1
Saccharomyces cerevisiae (baker's yeast) MBF1 NP_014942.4
Kluyveromyces lactis KLLA0F25014g XP_456195.2
Eremothecium gossypii AGOS_AFR526C NP_986073.1
Arabidopsis thaliana (thale cress) MBF1B NP_191427.1
Arabidopsis thaliana (thale cress) MBF1A NP_565981.1
Rattus norvegicus (Norway rat) Edf1 NP_001100027.1
EDF1 XP_001092893.1
Mus musculus (house mouse) Edf1 NP_067494.1
Danio rerio (zebrafish) edf1 NP_957039.1
Drosophila melanogaster (fruit fly) mbf1 NP_001261952.1
Caenorhabditis elegans (roundworm) mbf-1 NP_502166.1

Gene Ontology


Bibliography

Related articles in PubMed

Structure homology and interaction redundancy for discovering virus-host protein interactions.
de Chassey B, Meyniel-Schicklin L, Aublin-Gex A, Navratil V, Chantier T, Andr? P, Lotteau V
EMBO reports14(10)938-44(2013 Oct)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Human multiprotein bridging factor 1 and Calmodulin do not interact in vitro as confirmed by NMR spectroscopy and CaM-agarose affinity chromatography.
Babini E, Hu X, Parigi G, Vignali M
Protein expression and purification80(1)1-7(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

According to this study multiprotein bridging factor 1 and Calmodulin do not interact in vitro as confirmed by NMR spectroscopy and CaM-agarose affinity chromatography.
Title: Human multiprotein bridging factor 1 and Calmodulin do not interact in vitro as confirmed by NMR spectroscopy and CaM-agarose affinity chromatography.

The EDF-1 minimal promoter was characterized.
Title: Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription.

Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism.
Title: Multiprotein bridging factor-1 (MBF-1) is a cofactor for nuclear receptors that regulate lipid metabolism.

The following EDF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu06960 NM_153200.2
Latest version!		 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant beta, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu20848 NM_003792.4
Latest version!		 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu04777 NM_001281297.2
Latest version!		 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu04778 NM_001281299.2
Latest version!		 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu04779 NM_001281298.2
Latest version!		 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu04777 NM_001281297.1 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu04779 NM_001281298.1 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu20848 NM_003792.3 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant alpha, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu04778 NM_001281299.1 Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu06960
Clone ID Related Accession (Same CDS sequence) NM_153200.2
Accession Version NM_153200.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 420bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-15
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform beta
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1, BC015500.1, BI833652.1 and AA325420.1. On Jul 24, 2013 this sequence version replaced NM_153200.1. Transcript Variant: This variant (beta) differs its 3' UTR and 3' coding region, compared to variant alpha. The encoded isoform (beta) has a shorter and distinct C-terminus, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI833652.1, SRR1803614.403678.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGGTGAG TGTCCCTCCA CCCTTCGCCG GGTCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_694880.1
CDS79..498
Translation

Target ORF information:

RefSeq Version NM_153200.2
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant beta, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_153200.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGGTGAG TGTCCCTCCA CCCTTCGCCG GGTCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20848
Clone ID Related Accession (Same CDS sequence) NM_003792.3 , NM_003792.4
Accession Version NM_003792.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 447bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform alpha
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1, BC015500.1 and BM800379.1. This sequence is a reference standard in the RefSeqGene project. On Jul 24, 2013 this sequence version replaced NM_003792.2. Transcript Variant: This variant (alpha) encodes the longest isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM800379.1, SRR1163658.572084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGGCCTC AAGCTCCGGG GAAAGGACAT TGGAAAGCCC 
ATCGAGAAGG GGCCTAGGGC GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003783.1
CDS79..525
Translation

Target ORF information:

RefSeq Version NM_003792.3
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003792.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGGCCTC AAGCTCCGGG GAAAGGACAT TGGAAAGCCC 
ATCGAGAAGG GGCCTAGGGC GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20848
Clone ID Related Accession (Same CDS sequence) NM_003792.3 , NM_003792.4
Accession Version NM_003792.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 447bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-16
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform alpha
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1 and BC015500.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003792.3. Transcript Variant: This variant (alpha) encodes the longest isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.234522.1, SRR5189655.171420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000224073.6/ ENSP00000224073.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGGCCTC AAGCTCCGGG GAAAGGACAT TGGAAAGCCC 
ATCGAGAAGG GGCCTAGGGC GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003783.1
CDS51..497
Translation

Target ORF information:

RefSeq Version NM_003792.4
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant alpha, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003792.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGGCCTC AAGCTCCGGG GAAAGGACAT TGGAAAGCCC 
ATCGAGAAGG GGCCTAGGGC GAAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04777
Clone ID Related Accession (Same CDS sequence) NM_001281297.1 , NM_001281297.2
Accession Version NM_001281297.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-10-13
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1, BQ279031.1 and BM800379.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3'-terminal exon, which leads to a translational frameshift, compared to variant alpha. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI859811.1, BM423594.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGATGTG GGAACCAGGA GTGCTCGTGT GCTGAGAGCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001268226.1
CDS79..504
Translation

Target ORF information:

RefSeq Version NM_001281297.1
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001281297.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGATGTG GGAACCAGGA GTGCTCGTGT GCTGAGAGCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04777
Clone ID Related Accession (Same CDS sequence) NM_001281297.1 , NM_001281297.2
Accession Version NM_001281297.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 426bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-18
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1 and BQ279031.1. On Jun 1, 2019 this sequence version replaced NM_001281297.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3'-terminal exon, which leads to a translational frameshift, compared to variant alpha. The encoded isoform (3) has a shorter and distinct C-terminus, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.438804.1, SRR7410570.438789.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGATGTG GGAACCAGGA GTGCTCGTGT GCTGAGAGCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001268226.1
CDS51..476
Translation

Target ORF information:

RefSeq Version NM_001281297.2
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001281297.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG 
ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT 
GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG 
CTTGGCAAAA TCGAGCGGGC CATTGATGTG GGAACCAGGA GTGCTCGTGT GCTGAGAGCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04778
Clone ID Related Accession (Same CDS sequence) NM_001281299.1 , NM_001281299.2
Accession Version NM_001281299.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 393bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-10-13
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform 5
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1, BC015500.1, HY086994.1 and BM800379.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the central coding region, compared to variant alpha. The encoded isoform (5) is shorter, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY274852.1, HY086994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGAAA 
ATCAATGAGA AGCCACAGGT GATCGCGGAC TATGAGAGCG GACGGGCCAT ACCCAATAAC 
CAGGTGCTTG GCAAAATCGA GCGGGCCATT GGCCTCAAGC TCCGGGGAAA GGACATTGGA 
AAGCCCATCG AGAAGGGGCC TAGGGCGAAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001268228.1
CDS79..471
Translation

Target ORF information:

RefSeq Version NM_001281299.1
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001281299.1

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGAAA 
ATCAATGAGA AGCCACAGGT GATCGCGGAC TATGAGAGCG GACGGGCCAT ACCCAATAAC 
CAGGTGCTTG GCAAAATCGA GCGGGCCATT GGCCTCAAGC TCCGGGGAAA GGACATTGGA 
AAGCCCATCG AGAAGGGGCC TAGGGCGAAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04778
Clone ID Related Accession (Same CDS sequence) NM_001281299.1 , NM_001281299.2
Accession Version NM_001281299.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 393bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-18
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform 5
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1, BC015500.1 and HY086994.1. On Jun 1, 2019 this sequence version replaced NM_001281299.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the central coding region, compared to variant alpha. The encoded isoform (5) is shorter, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY274852.1, HY086994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGAAA 
ATCAATGAGA AGCCACAGGT GATCGCGGAC TATGAGAGCG GACGGGCCAT ACCCAATAAC 
CAGGTGCTTG GCAAAATCGA GCGGGCCATT GGCCTCAAGC TCCGGGGAAA GGACATTGGA 
AAGCCCATCG AGAAGGGGCC TAGGGCGAAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001268228.1
CDS51..443
Translation

Target ORF information:

RefSeq Version NM_001281299.2
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001281299.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGAAA 
ATCAATGAGA AGCCACAGGT GATCGCGGAC TATGAGAGCG GACGGGCCAT ACCCAATAAC 
CAGGTGCTTG GCAAAATCGA GCGGGCCATT GGCCTCAAGC TCCGGGGAAA GGACATTGGA 
AAGCCCATCG AGAAGGGGCC TAGGGCGAAA TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04779
Clone ID Related Accession (Same CDS sequence) NM_001281298.2 , NM_001281298.1
Accession Version NM_001281298.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-18
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform 4
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY170122.1 and BC015500.1. On May 31, 2019 this sequence version replaced NM_001281298.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the central coding region, compared to variant alpha. The encoded isoform (4) is shorter, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY170122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267778 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGAA AATCAATGAG 
AAGCCACAGG TGATCGCGGA CTATGAGAGC GGACGGGCCA TACCCAATAA CCAGGTGCTT 
GGCAAAATCG AGCGGGCCAT TGGCCTCAAG CTCCGGGGAA AGGACATTGG AAAGCCCATC 
GAGAAGGGGC CTAGGGCGAA ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001268227.1
CDS51..434
Translation

Target ORF information:

RefSeq Version NM_001281298.2
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001281298.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGAA AATCAATGAG 
AAGCCACAGG TGATCGCGGA CTATGAGAGC GGACGGGCCA TACCCAATAA CCAGGTGCTT 
GGCAAAATCG AGCGGGCCAT TGGCCTCAAG CTCCGGGGAA AGGACATTGG AAAGCCCATC 
GAGAAGGGGC CTAGGGCGAA ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu04779
Clone ID Related Accession (Same CDS sequence) NM_001281298.2 , NM_001281298.1
Accession Version NM_001281298.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 384bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-10-13
Organism Homo sapiens(human)
Product endothelial differentiation-related factor 1 isoform 4
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY029111.1, HY170122.1, BC015500.1 and BM800379.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the central coding region, compared to variant alpha. The encoded isoform (4) is shorter, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY170122.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267778 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGAA AATCAATGAG 
AAGCCACAGG TGATCGCGGA CTATGAGAGC GGACGGGCCA TACCCAATAA CCAGGTGCTT 
GGCAAAATCG AGCGGGCCAT TGGCCTCAAG CTCCGGGGAA AGGACATTGG AAAGCCCATC 
GAGAAGGGGC CTAGGGCGAA ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001268227.1
CDS79..462
Translation

Target ORF information:

RefSeq Version NM_001281298.1
Organism Homo sapiens(human)
Definition Homo sapiens endothelial differentiation related factor 1 (EDF1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001281298.1

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC 
CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT 
TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG 
CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGAA AATCAATGAG 
AAGCCACAGG TGATCGCGGA CTATGAGAGC GGACGGGCCA TACCCAATAA CCAGGTGCTT 
GGCAAAATCG AGCGGGCCAT TGGCCTCAAG CTCCGGGGAA AGGACATTGG AAAGCCCATC 
GAGAAGGGGC CTAGGGCGAA ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Structure homology and interaction redundancy for discovering virus-host protein interactions.
EMBO reports14(10)938-44(2013 Oct)
de Chassey B,Meyniel-Schicklin L,Aublin-Gex A,Navratil V,Chantier T,Andr? P,Lotteau V


book

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW


book

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M


book

Human multiprotein bridging factor 1 and Calmodulin do not interact in vitro as confirmed by NMR spectroscopy and CaM-agarose affinity chromatography.
Protein expression and purification80(1)1-7(2011 Nov)
Babini E,Hu X,Parigi G,Vignali M