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TNFRSF10B cDNA ORF clone, Homo sapiens(Human)

Gene Symbol TNFRSF10B
Entrez Gene ID 8795
Full Name TNF receptor superfamily member 10b
Synonyms CD262,DR5,KILLER,KILLER/DR5,TRAIL-R2,TRAILR2,TRICK2,TRICK2A,TRICK2B,TRICKB,ZTNFR9
General protein information
Preferred Names

TNF receptor superfamily member 10b

Names

tumor necrosis factor receptor superfamily member 10B
Fas-like protein
TNF-related apoptosis-inducing ligand receptor 2
apoptosis inducing protein TRICK2A/2B
apoptosis inducing receptor TRAIL-R2
cytotoxic TRAIL receptor-2
death domain containing receptor for TRAIL/Apo-2L
death receptor 5
p53-regulated DNA damage-inducible cell death receptor(killer)
tumor necrosis factor receptor superfamily, member 10b
tumor necrosis factor receptor-like protein ZTNFR9

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

8

8p21.3

Summary The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009].
Disorder MIM:

603612

Disorder Html: Squamous cell carcinoma, head and neck, 275355 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_003842.4 NP_003833.4 tumor necrosis factor receptor superfamily member 10B isoform 1 precursor
XM_017013944.1 XP_016869433.1 tumor necrosis factor receptor superfamily member 10B isoform X1
NM_147187.2 NP_671716.2 tumor necrosis factor receptor superfamily member 10B isoform 2 precursor



Pan troglodytes (chimpanzee) TNFRSF10B XP_001158074.1
Gallus gallus (chicken) TNFRSF10B NP_989446.2
Xenopus tropicalis (tropical clawed frog) tnfrsf10b NP_001004894.1
Homo sapiens (human) TNFRSF10B NP_003833.4
Macaca mulatta (rhesus monkey) LOC716650 XP_001107490.2


Related articles in PubMed

Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.
Yang J, Li G, Zhang K
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie841078-1087(2016 Dec)

An HPLC method associated with a thermodynamic analysis to compare the binding of TRAIL and its nanovectorized form to death receptors DR4 and DR5 and their relationship to cytotoxicity.
Guillaume YC, Lethier L, André C
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences1036-1037142-148(2016 Nov)

Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway.
Zhu W, Zhan D, Wang L, Ma D, Cheng M, Wang H, Zhao J, Cai Y, Cheng Z
Oncology reports36(2)845-52(2016 Aug)

The pyrrolo-1,5-benzoxazepine, PBOX-15, enhances TRAIL‑induced apoptosis by upregulation of DR5 and downregulation of core cell survival proteins in acute lymphoblastic leukaemia cells.
Nathwani SM, Greene LM, Butini S, Campiani G, Williams DC, Samali A, Szegezdi E, Zisterer DM
International journal of oncology49(1)74-88(2016 Jul)

Caffeic acid phenethyl ester enhances TRAIL-mediated apoptosis via CHOP-induced death receptor 5 upregulation in hepatocarcinoma Hep3B cells.
Dilshara MG, Jayasooriya RG, Park SR, Choi YH, Choi IW, Kim GY
Molecular and cellular biochemistry418(1-2)13-20(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study provides direct biophysical evidence that Death Receptor 5 disulfide-linked transmembrane (TM)-dimers open in response to ligand binding. Then, to probe the importance of the closed-to-open TM domain transition in the overall energetics of receptor activation, point-mutants (alanine to phenylalanine) in the predicted, tightly packed TM domain dimer interface were designed and tested.
Title: Death Receptor 5 Activation Is Energetically Coupled to Opening of the Transmembrane Domain Dimer.

Mono treatment with lexatumumab was not sufficient to induce apoptosis in pancreatic cancer cells, whereas focal adhesion kinase inhibitor PF573228 significantly sensitized lexatumumab-induced apoptosis. Western blotting analysis revealed that lexatumumab and PF573228 combination treatment increased death receptor 5 but decreased Bcl-xL expression.
Title: Focal adhesion kinase inhibitor PF573228 and death receptor 5 agonist lexatumumab synergistically induce apoptosis in pancreatic carcinoma.

High-order TRAIL oligomer formation in TRAIL-coated lipid nanoparticles enhances DR5 cross-linking and increases antitumour effect against colon cancer cells and xenograft tumors.
Title: High-order TRAIL oligomer formation in TRAIL-coated lipid nanoparticles enhances DR5 cross-linking and increases antitumour effect against colon cancer.

Pro-survival effects by NF-kappaB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer, which may explain why up-regulated DR4 and DR5 in ovarian cancer are associated with poor prognosis and low survival ratio of the patients.
Title: Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.

The nanovectorization of TRAIL enhanced its binding to both DR4 and DR5 receptors at 37 degrees C and could potentially sensitized cancer cells to TRAIL induced apoptosis through simultaneous activation of DR4 and DR5 as described in this paper for the non-small lung carcinoma cell line (H1703), the two hepatocarcinoma cell lines (SK-Hep1, HUH) and the colon carcinoma cell line (HCT116WT).
Title: An HPLC method associated with a thermodynamic analysis to compare the binding of TRAIL and its nanovectorized form to death receptors DR4 and DR5 and their relationship to cytotoxicity.

The following TNFRSF10B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNFRSF10B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu15833 NM_147187.2
Latest version!
Homo sapiens TNF receptor superfamily member 10b (TNFRSF10B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $244.30
$349.00
OHu22693 NM_003842.4
Latest version!
Homo sapiens TNF receptor superfamily member 10b (TNFRSF10B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu98267 XM_017013944.1
Latest version!
Homo sapiens tumor necrosis factor receptor superfamily member 10b (TNFRSF10B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15833
Clone ID Related Accession (Same CDS sequence) NM_147187.2
Accession Version NM_147187.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1236bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-09
Organism Homo sapiens(Human)
Product tumor necrosis factor receptor superfamily member 10B isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA738472.1, DA889488.1, BC001281.1, AF192548.1 and AC107959.8. On Mar 4, 2009 this sequence version replaced NM_147187.1. Transcript Variant: This variant (2) lacks an in-frame coding segment compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.78005.1, SRR1803617.244835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAACAAC GGGGACAGAA CGCCCCGGCC GCTTCGGGGG CCCGGAAAAG GCACGGCCCA 
GGACCCAGGG AGGCGCGGGG AGCCAGGCCT GGGCCCCGGG TCCCCAAGAC CCTTGTGCTC
GTTGTCGCCG CGGTCCTGCT GTTGGTCTCA GCTGAGTCTG CTCTGATCAC CCAACAAGAC
CTAGCTCCCC AGCAGAGAGC GGCCCCACAA CAAAAGAGGT CCAGCCCCTC AGAGGGATTG
TGTCCACCTG GACACCATAT CTCAGAAGAC GGTAGAGATT GCATCTCCTG CAAATATGGA
CAGGACTATA GCACTCACTG GAATGACCTC CTTTTCTGCT TGCGCTGCAC CAGGTGTGAT
TCAGGTGAAG TGGAGCTAAG TCCCTGCACC ACGACCAGAA ACACAGTGTG TCAGTGCGAA
GAAGGCACCT TCCGGGAAGA AGATTCTCCT GAGATGTGCC GGAAGTGCCG CACAGGGTGT
CCCAGAGGGA TGGTCAAGGT CGGTGATTGT ACACCCTGGA GTGACATCGA ATGTGTCCAC
AAAGAATCAG GCATCATCAT AGGAGTCACA GTTGCAGCCG TAGTCTTGAT TGTGGCTGTG
TTTGTTTGCA AGTCTTTACT GTGGAAGAAA GTCCTTCCTT ACCTGAAAGG CATCTGCTCA
GGTGGTGGTG GGGACCCTGA GCGTGTGGAC AGAAGCTCAC AACGACCTGG GGCTGAGGAC
AATGTCCTCA ATGAGATCGT GAGTATCTTG CAGCCCACCC AGGTCCCTGA GCAGGAAATG
GAAGTCCAGG AGCCAGCAGA GCCAACAGGT GTCAACATGT TGTCCCCCGG GGAGTCAGAG
CATCTGCTGG AACCGGCAGA AGCTGAAAGG TCTCAGAGGA GGAGGCTGCT GGTTCCAGCA
AATGAAGGTG ATCCCACTGA GACTCTGAGA CAGTGCTTCG ATGACTTTGC AGACTTGGTG
CCCTTTGACT CCTGGGAGCC GCTCATGAGG AAGTTGGGCC TCATGGACAA TGAGATAAAG
GTGGCTAAAG CTGAGGCAGC GGGCCACAGG GACACCTTGT ACACGATGCT GATAAAGTGG
GTCAACAAAA CCGGGCGAGA TGCCTCTGTC CACACCCTGC TGGATGCCTT GGAGACGCTG
GGAGAGAGAC TTGCCAAGCA GAAGATTGAG GACCACTTGT TGAGCTCTGG AAAGTTCATG
TATCTAGAAG GTAATGCAGA CTCTGCCATG TCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_671716.2
CDS294..1529
Misc Feature(1)264..266
Misc Feature(2)522..830
Misc Feature(3)522..575
Misc Feature(4)order(525..533,552..554,558..560,609..614,618..629,633..638,753..755,816..818)
Misc Feature(5)582..704
Misc Feature(6)order(627..629,636..638,651..653,744..758)
Misc Feature(7)708..827
Misc Feature(8)1227..1490
Exon (1)1..437
Exon (2)438..543
Exon (3)544..657
Exon (4)658..769
Exon (5)770..843
Exon (6)844..954
Exon (7)955..986
Exon (8)987..1142
Exon (9)1143..1215
Exon (10)1216..4067
Translation

Target ORF information:

RefSeq Version NM_147187.2
Organism Homo sapiens(Human)
Definition Homo sapiens TNF receptor superfamily member 10b (TNFRSF10B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_147187.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGAACAAC GGGGACAGAA CGCCCCGGCC GCTTCGGGGG CCCGGAAAAG GCACGGCCCA 
GGACCCAGGG AGGCGCGGGG AGCCAGGCCT GGGCCCCGGG TCCCCAAGAC CCTTGTGCTC
GTTGTCGCCG CGGTCCTGCT GTTGGTCTCA GCTGAGTCTG CTCTGATCAC CCAACAAGAC
CTAGCTCCCC AGCAGAGAGC GGCCCCACAA CAAAAGAGGT CCAGCCCCTC AGAGGGATTG
TGTCCACCTG GACACCATAT CTCAGAAGAC GGTAGAGATT GCATCTCCTG CAAATATGGA
CAGGACTATA GCACTCACTG GAATGACCTC CTTTTCTGCT TGCGCTGCAC CAGGTGTGAT
TCAGGTGAAG TGGAGCTAAG TCCCTGCACC ACGACCAGAA ACACAGTGTG TCAGTGCGAA
GAAGGCACCT TCCGGGAAGA AGATTCTCCT GAGATGTGCC GGAAGTGCCG CACAGGGTGT
CCCAGAGGGA TGGTCAAGGT CGGTGATTGT ACACCCTGGA GTGACATCGA ATGTGTCCAC
AAAGAATCAG GCATCATCAT AGGAGTCACA GTTGCAGCCG TAGTCTTGAT TGTGGCTGTG
TTTGTTTGCA AGTCTTTACT GTGGAAGAAA GTCCTTCCTT ACCTGAAAGG CATCTGCTCA
GGTGGTGGTG GGGACCCTGA GCGTGTGGAC AGAAGCTCAC AACGACCTGG GGCTGAGGAC
AATGTCCTCA ATGAGATCGT GAGTATCTTG CAGCCCACCC AGGTCCCTGA GCAGGAAATG
GAAGTCCAGG AGCCAGCAGA GCCAACAGGT GTCAACATGT TGTCCCCCGG GGAGTCAGAG
CATCTGCTGG AACCGGCAGA AGCTGAAAGG TCTCAGAGGA GGAGGCTGCT GGTTCCAGCA
AATGAAGGTG ATCCCACTGA GACTCTGAGA CAGTGCTTCG ATGACTTTGC AGACTTGGTG
CCCTTTGACT CCTGGGAGCC GCTCATGAGG AAGTTGGGCC TCATGGACAA TGAGATAAAG
GTGGCTAAAG CTGAGGCAGC GGGCCACAGG GACACCTTGT ACACGATGCT GATAAAGTGG
GTCAACAAAA CCGGGCGAGA TGCCTCTGTC CACACCCTGC TGGATGCCTT GGAGACGCTG
GGAGAGAGAC TTGCCAAGCA GAAGATTGAG GACCACTTGT TGAGCTCTGG AAAGTTCATG
TATCTAGAAG GTAATGCAGA CTCTGCCATG TCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22693
Clone ID Related Accession (Same CDS sequence) NM_003842.4
Accession Version NM_003842.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1323bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product tumor necrosis factor receptor superfamily member 10B isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA738472.1, DA889488.1, BC001281.1, CX870630.1, BX537617.1 and AC107959.8. This sequence is a reference standard in the RefSeqGene project. On Mar 4, 2009 this sequence version replaced NM_003842.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF016266.1, SRR1803611.65409.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAACAAC GGGGACAGAA CGCCCCGGCC GCTTCGGGGG CCCGGAAAAG GCACGGCCCA 
GGACCCAGGG AGGCGCGGGG AGCCAGGCCT GGGCCCCGGG TCCCCAAGAC CCTTGTGCTC
GTTGTCGCCG CGGTCCTGCT GTTGGTCTCA GCTGAGTCTG CTCTGATCAC CCAACAAGAC
CTAGCTCCCC AGCAGAGAGC GGCCCCACAA CAAAAGAGGT CCAGCCCCTC AGAGGGATTG
TGTCCACCTG GACACCATAT CTCAGAAGAC GGTAGAGATT GCATCTCCTG CAAATATGGA
CAGGACTATA GCACTCACTG GAATGACCTC CTTTTCTGCT TGCGCTGCAC CAGGTGTGAT
TCAGGTGAAG TGGAGCTAAG TCCCTGCACC ACGACCAGAA ACACAGTGTG TCAGTGCGAA
GAAGGCACCT TCCGGGAAGA AGATTCTCCT GAGATGTGCC GGAAGTGCCG CACAGGGTGT
CCCAGAGGGA TGGTCAAGGT CGGTGATTGT ACACCCTGGA GTGACATCGA ATGTGTCCAC
AAAGAATCAG GTACAAAGCA CAGTGGGGAA GTCCCAGCTG TGGAGGAGAC GGTGACCTCC
AGCCCAGGGA CTCCTGCCTC TCCCTGTTCT CTCTCAGGCA TCATCATAGG AGTCACAGTT
GCAGCCGTAG TCTTGATTGT GGCTGTGTTT GTTTGCAAGT CTTTACTGTG GAAGAAAGTC
CTTCCTTACC TGAAAGGCAT CTGCTCAGGT GGTGGTGGGG ACCCTGAGCG TGTGGACAGA
AGCTCACAAC GACCTGGGGC TGAGGACAAT GTCCTCAATG AGATCGTGAG TATCTTGCAG
CCCACCCAGG TCCCTGAGCA GGAAATGGAA GTCCAGGAGC CAGCAGAGCC AACAGGTGTC
AACATGTTGT CCCCCGGGGA GTCAGAGCAT CTGCTGGAAC CGGCAGAAGC TGAAAGGTCT
CAGAGGAGGA GGCTGCTGGT TCCAGCAAAT GAAGGTGATC CCACTGAGAC TCTGAGACAG
TGCTTCGATG ACTTTGCAGA CTTGGTGCCC TTTGACTCCT GGGAGCCGCT CATGAGGAAG
TTGGGCCTCA TGGACAATGA GATAAAGGTG GCTAAAGCTG AGGCAGCGGG CCACAGGGAC
ACCTTGTACA CGATGCTGAT AAAGTGGGTC AACAAAACCG GGCGAGATGC CTCTGTCCAC
ACCCTGCTGG ATGCCTTGGA GACGCTGGGA GAGAGACTTG CCAAGCAGAA GATTGAGGAC
CACTTGTTGA GCTCTGGAAA GTTCATGTAT CTAGAAGGTA ATGCAGACTC TGCCATGTCC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003833.4
CDS294..1616
Misc Feature(1)264..266
Misc Feature(2)462..575
Misc Feature(3)522..830
Misc Feature(4)522..575
Misc Feature(5)order(525..533,552..554,558..560,609..614,618..629,633..638,753..755,816..818)
Misc Feature(6)582..704
Misc Feature(7)582..704
Misc Feature(8)order(627..629,636..638,651..653,744..758)
Misc Feature(9)705..827
Misc Feature(10)708..827
Misc Feature(11)867..911
Misc Feature(12)924..986
Misc Feature(13)1314..1577
Exon (1)1..437
Exon (2)438..543
Exon (3)544..657
Exon (4)658..769
Exon (5)770..1041
Exon (6)1042..1073
Exon (7)1074..1229
Exon (8)1230..1302
Exon (9)1303..4154
Translation

Target ORF information:

RefSeq Version NM_003842.4
Organism Homo sapiens(Human)
Definition Homo sapiens TNF receptor superfamily member 10b (TNFRSF10B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003842.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAACAAC GGGGACAGAA CGCCCCGGCC GCTTCGGGGG CCCGGAAAAG GCACGGCCCA 
GGACCCAGGG AGGCGCGGGG AGCCAGGCCT GGGCCCCGGG TCCCCAAGAC CCTTGTGCTC
GTTGTCGCCG CGGTCCTGCT GTTGGTCTCA GCTGAGTCTG CTCTGATCAC CCAACAAGAC
CTAGCTCCCC AGCAGAGAGC GGCCCCACAA CAAAAGAGGT CCAGCCCCTC AGAGGGATTG
TGTCCACCTG GACACCATAT CTCAGAAGAC GGTAGAGATT GCATCTCCTG CAAATATGGA
CAGGACTATA GCACTCACTG GAATGACCTC CTTTTCTGCT TGCGCTGCAC CAGGTGTGAT
TCAGGTGAAG TGGAGCTAAG TCCCTGCACC ACGACCAGAA ACACAGTGTG TCAGTGCGAA
GAAGGCACCT TCCGGGAAGA AGATTCTCCT GAGATGTGCC GGAAGTGCCG CACAGGGTGT
CCCAGAGGGA TGGTCAAGGT CGGTGATTGT ACACCCTGGA GTGACATCGA ATGTGTCCAC
AAAGAATCAG GTACAAAGCA CAGTGGGGAA GTCCCAGCTG TGGAGGAGAC GGTGACCTCC
AGCCCAGGGA CTCCTGCCTC TCCCTGTTCT CTCTCAGGCA TCATCATAGG AGTCACAGTT
GCAGCCGTAG TCTTGATTGT GGCTGTGTTT GTTTGCAAGT CTTTACTGTG GAAGAAAGTC
CTTCCTTACC TGAAAGGCAT CTGCTCAGGT GGTGGTGGGG ACCCTGAGCG TGTGGACAGA
AGCTCACAAC GACCTGGGGC TGAGGACAAT GTCCTCAATG AGATCGTGAG TATCTTGCAG
CCCACCCAGG TCCCTGAGCA GGAAATGGAA GTCCAGGAGC CAGCAGAGCC AACAGGTGTC
AACATGTTGT CCCCCGGGGA GTCAGAGCAT CTGCTGGAAC CGGCAGAAGC TGAAAGGTCT
CAGAGGAGGA GGCTGCTGGT TCCAGCAAAT GAAGGTGATC CCACTGAGAC TCTGAGACAG
TGCTTCGATG ACTTTGCAGA CTTGGTGCCC TTTGACTCCT GGGAGCCGCT CATGAGGAAG
TTGGGCCTCA TGGACAATGA GATAAAGGTG GCTAAAGCTG AGGCAGCGGG CCACAGGGAC
ACCTTGTACA CGATGCTGAT AAAGTGGGTC AACAAAACCG GGCGAGATGC CTCTGTCCAC
ACCCTGCTGG ATGCCTTGGA GACGCTGGGA GAGAGACTTG CCAAGCAGAA GATTGAGGAC
CACTTGTTGA GCTCTGGAAA GTTCATGTAT CTAGAAGGTA ATGCAGACTC TGCCATGTCC
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98267
Clone ID Related Accession (Same CDS sequence) XM_017013944.1
Accession Version XM_017013944.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1128bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product tumor necrosis factor receptor superfamily member 10B isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGATGACG TGTGGGGAAT GAGGACAATC GAGGCACCAA ATTCAACTGC TCAGGGACAC 
CATATCTCAG AAGACGGTAG AGATTGCATC TCCTGCAAAT ATGGACAGGA CTATAGCACT
CACTGGAATG ACCTCCTTTT CTGCTTGCGC TGCACCAGGT GTGATTCAGG TGAAGTGGAG
CTAAGTCCCT GCACCACGAC CAGAAACACA GTGTGTCAGT GCGAAGAAGG CACCTTCCGG
GAAGAAGATT CTCCTGAGAT GTGCCGGAAG TGCCGCACAG GGTGTCCCAG AGGGATGGTC
AAGGTCGGTG ATTGTACACC CTGGAGTGAC ATCGAATGTG TCCACAAAGA ATCAGGTACA
AAGCACAGTG GGGAAGTCCC AGCTGTGGAG GAGACGGTGA CCTCCAGCCC AGGGACTCCT
GCCTCTCCCT GTTCTCTCTC AGGCATCATC ATAGGAGTCA CAGTTGCAGC CGTAGTCTTG
ATTGTGGCTG TGTTTGTTTG CAAGTCTTTA CTGTGGAAGA AAGTCCTTCC TTACCTGAAA
GGCATCTGCT CAGGTGGTGG TGGGGACCCT GAGCGTGTGG ACAGAAGCTC ACAACGACCT
GGGGCTGAGG ACAATGTCCT CAATGAGATC GTGAGTATCT TGCAGCCCAC CCAGGTCCCT
GAGCAGGAAA TGGAAGTCCA GGAGCCAGCA GAGCCAACAG GTGTCAACAT GTTGTCCCCC
GGGGAGTCAG AGCATCTGCT GGAACCGGCA GAAGCTGAAA GGTCTCAGAG GAGGAGGCTG
CTGGTTCCAG CAAATGAAGG TGATCCCACT GAGACTCTGA GACAGTGCTT CGATGACTTT
GCAGACTTGG TGCCCTTTGA CTCCTGGGAG CCGCTCATGA GGAAGTTGGG CCTCATGGAC
AATGAGATAA AGGTGGCTAA AGCTGAGGCA GCGGGCCACA GGGACACCTT GTACACGATG
CTGATAAAGT GGGTCAACAA AACCGGGCGA GATGCCTCTG TCCACACCCT GCTGGATGCC
TTGGAGACGC TGGGAGAGAG ACTTGCCAAG CAGAAGATTG AGGACCACTT GTTGAGCTCT
GGAAAGTTCA TGTATCTAGA AGGTAATGCA GACTCTGCCA TGTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869433.1
CDS277..1404
Translation

Target ORF information:

RefSeq Version XM_017013944.1
Organism Homo sapiens(Human)
Definition Homo sapiens tumor necrosis factor receptor superfamily member 10b (TNFRSF10B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013944.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGATGACG TGTGGGGAAT GAGGACAATC GAGGCACCAA ATTCAACTGC TCAGGGACAC 
CATATCTCAG AAGACGGTAG AGATTGCATC TCCTGCAAAT ATGGACAGGA CTATAGCACT
CACTGGAATG ACCTCCTTTT CTGCTTGCGC TGCACCAGGT GTGATTCAGG TGAAGTGGAG
CTAAGTCCCT GCACCACGAC CAGAAACACA GTGTGTCAGT GCGAAGAAGG CACCTTCCGG
GAAGAAGATT CTCCTGAGAT GTGCCGGAAG TGCCGCACAG GGTGTCCCAG AGGGATGGTC
AAGGTCGGTG ATTGTACACC CTGGAGTGAC ATCGAATGTG TCCACAAAGA ATCAGGTACA
AAGCACAGTG GGGAAGTCCC AGCTGTGGAG GAGACGGTGA CCTCCAGCCC AGGGACTCCT
GCCTCTCCCT GTTCTCTCTC AGGCATCATC ATAGGAGTCA CAGTTGCAGC CGTAGTCTTG
ATTGTGGCTG TGTTTGTTTG CAAGTCTTTA CTGTGGAAGA AAGTCCTTCC TTACCTGAAA
GGCATCTGCT CAGGTGGTGG TGGGGACCCT GAGCGTGTGG ACAGAAGCTC ACAACGACCT
GGGGCTGAGG ACAATGTCCT CAATGAGATC GTGAGTATCT TGCAGCCCAC CCAGGTCCCT
GAGCAGGAAA TGGAAGTCCA GGAGCCAGCA GAGCCAACAG GTGTCAACAT GTTGTCCCCC
GGGGAGTCAG AGCATCTGCT GGAACCGGCA GAAGCTGAAA GGTCTCAGAG GAGGAGGCTG
CTGGTTCCAG CAAATGAAGG TGATCCCACT GAGACTCTGA GACAGTGCTT CGATGACTTT
GCAGACTTGG TGCCCTTTGA CTCCTGGGAG CCGCTCATGA GGAAGTTGGG CCTCATGGAC
AATGAGATAA AGGTGGCTAA AGCTGAGGCA GCGGGCCACA GGGACACCTT GTACACGATG
CTGATAAAGT GGGTCAACAA AACCGGGCGA GATGCCTCTG TCCACACCCT GCTGGATGCC
TTGGAGACGC TGGGAGAGAG ACTTGCCAAG CAGAAGATTG AGGACCACTT GTTGAGCTCT
GGAAAGTTCA TGTATCTAGA AGGTAATGCA GACTCTGCCA TGTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie841078-1087(2016 Dec)
Yang J,Li G,Zhang K


book

An HPLC method associated with a thermodynamic analysis to compare the binding of TRAIL and its nanovectorized form to death receptors DR4 and DR5 and their relationship to cytotoxicity.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences1036-1037142-148(2016 Nov)
Guillaume YC,Lethier L,André C


book

Proteasome inhibitor MG132 potentiates TRAIL-induced apoptosis in gallbladder carcinoma GBC-SD cells via DR5-dependent pathway.
Oncology reports36(2)845-52(2016 Aug)
Zhu W,Zhan D,Wang L,Ma D,Cheng M,Wang H,Zhao J,Cai Y,Cheng Z


book

The pyrrolo-1,5-benzoxazepine, PBOX-15, enhances TRAIL‑induced apoptosis by upregulation of DR5 and downregulation of core cell survival proteins in acute lymphoblastic leukaemia cells.
International journal of oncology49(1)74-88(2016 Jul)
Nathwani SM,Greene LM,Butini S,Campiani G,Williams DC,Samali A,Szegezdi E,Zisterer DM


book

Caffeic acid phenethyl ester enhances TRAIL-mediated apoptosis via CHOP-induced death receptor 5 upregulation in hepatocarcinoma Hep3B cells.
Molecular and cellular biochemistry418(1-2)13-20(2016 Jul)
Dilshara MG,Jayasooriya RG,Park SR,Choi YH,Choi IW,Kim GY


 
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