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EIF2B5 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol EIF2B5
Entrez Gene ID 8893
Full Name eukaryotic translation initiation factor 2B subunit epsilon
Synonyms CACH,CLE,EIF-2B,EIF2Bepsilon,LVWM
General protein information
Preferred Names

eukaryotic translation initiation factor 2B subunit epsilon

Names

translation initiation factor eIF-2B subunit epsilon
eIF-2B GDP-GTP exchange factor subunit epsilon
eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

3

3q27.1
Summary This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq, Nov 2009].
Disorder MIM:

603945

Disorder Html: Leukoencephalopathy with vanishing white matter, 603896 (3); Ovarioleukodystrophy, 603896 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011513266.3 XP_011511568.1 translation initiation factor eIF-2B subunit epsilon isoform X2
NM_003907.2 NP_003898.2 translation initiation factor eIF-2B subunit epsilon
XM_011513266.2 XP_011511568.1 translation initiation factor eIF-2B subunit epsilon isoform X2
NM_003907.3 NP_003898.2 translation initiation factor eIF-2B subunit epsilon
XM_011513265.1 XP_011511567.1 translation initiation factor eIF-2B subunit epsilon isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) EIF2B5 XP_001139517.1
Gallus gallus (chicken) EIF2B5 XP_422755.3
Drosophila melanogaster (fruit fly) eIF2B-epsilon NP_996329.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000082 XP_001687758.1
Arabidopsis thaliana (thale cress) AT2G34970 NP_181042.1
EIF2B5 XP_001104122.1
Danio rerio (zebrafish) eif2b5 NP_001107069.1
Schizosaccharomyces pombe (fission yeast) tif225 NP_594285.1
Arabidopsis thaliana (thale cress) AT4G18300 NP_193564.1
Xenopus tropicalis (tropical clawed frog) eif2b5 XP_002935185.2
Mus musculus (house mouse) Eif2b5 NP_758469.1
Saccharomyces cerevisiae (baker's yeast) GCD6 NP_010497.3
Eremothecium gossypii AGOS_AGL094W NP_986572.1
Canis lupus familiaris (dog) EIF2B5 XP_003640192.1
Bos taurus (cattle) EIF2B5 XP_002684924.1
Rattus norvegicus (Norway rat) Eif2b5 NP_620221.2
Kluyveromyces lactis KLLA0A04235g XP_451187.1
Neurospora crassa NCU02414 XP_959596.1
Homo sapiens (human) EIF2B5 NP_003898.2
Magnaporthe oryzae (rice blast fungus) MGG_03517 XP_003716402.1
Arabidopsis thaliana (thale cress) AT3G02270 NP_186876.1
Oryza sativa (rice) Os02g0812400 NP_001048478.1

Gene Ontology


Bibliography

Related articles in PubMed

Transcriptome analysis of hypoxic cancer cells uncovers intron retention in EIF2B5 as a mechanism to inhibit translation.
Brady LK, Wang H, Radens CM, Bi Y, Radovich M, Maity A, Ivan C, Ivan M, Barash Y, Koumenis C
PLoS biology15(9)e2002623(2017 Sep)

No evidence for a role of Ile587Val polymorphism of EIF2B5 gene in multiple sclerosis in Kashmir Valley of India.
Zahoor I, Asimi R, Haq E
Journal of the neurological sciences359(1-2)172-6(2015 Dec)

A Japanese girl with an early-infantile onset vanishing white matter disease resembling Cree leukoencephalopathy.
Takano K, Tsuyusaki Y, Sato M, Takagi M, Anzai R, Okuda M, Iai M, Yamashita S, Okabe T, Aida N, Tsurusaki Y, Saitsu H, Matsumoto N, Osaka H
Brain & development37(6)638-42(2015 Jun)

Novel mutations identified in EIF2B5 gene in Kashmiri patients as susceptibility factor for multiple sclerosis.
Zahoor I, Hamid Z, Asimi R, Haq E
Indian journal of biochemistry & biophysics51(2)115-20(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exon-level analyses classified > 1,000 mRNAs as alternatively spliced under hypoxia and uncovered a unique retained intron (RI) in the master regulator of translation initiation, EIF2B5.
Title: Transcriptome analysis of hypoxic cancer cells uncovers intron retention in EIF2B5 as a mechanism to inhibit translation.

This study demonstrated that no evidence for a role of Ile587Val polymorphism of EIF2B5 gene in multiple sclerosis in Kashmir Valley if India.
Title: No evidence for a role of Ile587Val polymorphism of EIF2B5 gene in multiple sclerosis in Kashmir Valley of India.

Patient exhibits an early-infantile onset and progressive disease course resembling Cree leukoencephalopathy, suggesting a severe functional disruption of eIF2Bepsilon caused by R195H as well as by I408T mutations.
Title: A Japanese girl with an early-infantile onset vanishing white matter disease resembling Cree leukoencephalopathy.

Missense mutations in EIF2B5 are associated with multiple sclerosis.
Title: Novel mutations identified in EIF2B5 gene in Kashmiri patients as susceptibility factor for multiple sclerosis.

Also a mutation c.1913G>A[p.Arg638 His] in exon 14 of the EIF2B5-Gens as single nucleotide polymorphism.
Title: [Vanishing white matter disease: a stress-related leukodystrophy].

The following EIF2B5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu62293 XM_011513265.1
Latest version!		 Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu62294 XM_011513266.3
Latest version!		 Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu21417 NM_003907.3
Latest version!		 Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
View Old Accession Versions >>
OHu62294 XM_011513266.2 Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu21417 NM_003907.2 Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu62293
Clone ID Related Accession (Same CDS sequence) XM_011513265.1
Accession Version XM_011513265.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product translation initiation factor eIF-2B subunit epsilon isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCTGGGAT TACAGGTGGC ACAACTCTTT ACAGACAACT TTGACTACCA AACTCGAGAT 
GACTTTGTGC GAGGTCTCTT AGTGAATGAG GAGATCCTAG GGAACCAGAT CCACATGCAC 
GTAACAGCTA AGGAATATGG TGCCCGTGTC TCCAACCTAC ACATGTACTC AGCTGTCTGT 
GCTGACGTCA TCCGCCGATG GGTCTACCCT CTCACCCCAG AGGCGAACTT CACTGACAGC 
ACCACCCAGA GCTGCACTCA TTCCCGGCAC AACATCTACC GAGGGCCTGA GGTCAGCCTG 
GGCCATGGCA GCATCCTAGA GGAAAATGTG CTCCTGGGCT CTGGCACTGT CATTGGCAGC 
AATTGCTTTA TCACCAACAG TGTCATTGGC CCCGGCTGCC ACATTGGTGA TAACGTGGTG 
CTGGACCAGA CCTACCTGTG GCAGGGTGTT CGAGTGGCGG CTGGAGCACA GATCCATCAG 
TCTCTGCTTT GTGACAATGC TGAGGTCAAG GAACGAGTGA CACTGAAACC ACGCTCTGTC 
CTCACTTCCC AGGTGGTCGT GGGCCCAAAT ATCACGCTGC CTGAGGGCTC GGTGATCTCT 
TTGCACCCTC CAGATGCAGA GGAAGATGAA GATGATGGCG AGTTCAGTGA TGATTCTGGG 
GCTGACCAAG AAAAGGACAA AGTGAAGATG AAAGGTTACA ATCCAGCAGA AGTAGGAGCT 
GCTGGCAAGG GCTACCTCTG GAAAGCTGCA GGCATGAACA TGGAGGAAGA GGAGGAACTG 
CAGCAGAATC TGTGGGGACT CAAGATCAAC ATGGAAGAAG AGAGTGAAAG TGAAAGTGAG 
CAAAGTATGG ATTCTGAGGA GCCGGACAGC CGGGGAGGCT CCCCTCAGAT GGATGACATC 
AAAGTGTTCC AGAATGAAGT TTTAGGAACA CTACAGCGGG GCAAAGAGGA GAACATTTCT 
TGTGACAATC TCGTCCTGGA AATCAACTCT CTCAAGTATG CCTATAACAT AAGTCTAAAG 
GAGGTGATGC AGGTACTGAG CCACGTGGTC CTGGAGTTCC CCCTGCAACA GATGGATTCC 
CCGCTTGACT CAAGCCGCTA CTGTGCCCTG CTGCTTCCTC TGCTAAAGGC CTGGAGCCCT 
GTTTTTAGGA ACTACATAAA GCGCGCAGCC GACCATTTGG AAGCGTTAGC AGCCATTGAG 
GACTTCTTCC TAGAGCATGA AGCTCTTGGT ATTTCCATGG CCAAGGTACT GATGGCTTTC 
TACCAGCTGG AGATCCTGGC TGAGGAAACA ATTCTGAGCT GGTTCAGCCA AAGAGATACA 
ACTGACAAGG GCCAGCAGTT GCGCAAGAAT CAACAGCTGC AGAGGTTCAT CCAGTGGCTA 
AAAGAGGCAG AAGAGGAGTC ATCTGAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511567.1
CDS30..1445
Translation

Target ORF information:

RefSeq Version XM_011513265.1
Organism Homo sapiens(human)
Definition Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011513265.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCTGGGAT TACAGGTGGC ACAACTCTTT ACAGACAACT TTGACTACCA AACTCGAGAT 
GACTTTGTGC GAGGTCTCTT AGTGAATGAG GAGATCCTAG GGAACCAGAT CCACATGCAC 
GTAACAGCTA AGGAATATGG TGCCCGTGTC TCCAACCTAC ACATGTACTC AGCTGTCTGT 
GCTGACGTCA TCCGCCGATG GGTCTACCCT CTCACCCCAG AGGCGAACTT CACTGACAGC 
ACCACCCAGA GCTGCACTCA TTCCCGGCAC AACATCTACC GAGGGCCTGA GGTCAGCCTG 
GGCCATGGCA GCATCCTAGA GGAAAATGTG CTCCTGGGCT CTGGCACTGT CATTGGCAGC 
AATTGCTTTA TCACCAACAG TGTCATTGGC CCCGGCTGCC ACATTGGTGA TAACGTGGTG 
CTGGACCAGA CCTACCTGTG GCAGGGTGTT CGAGTGGCGG CTGGAGCACA GATCCATCAG 
TCTCTGCTTT GTGACAATGC TGAGGTCAAG GAACGAGTGA CACTGAAACC ACGCTCTGTC 
CTCACTTCCC AGGTGGTCGT GGGCCCAAAT ATCACGCTGC CTGAGGGCTC GGTGATCTCT 
TTGCACCCTC CAGATGCAGA GGAAGATGAA GATGATGGCG AGTTCAGTGA TGATTCTGGG 
GCTGACCAAG AAAAGGACAA AGTGAAGATG AAAGGTTACA ATCCAGCAGA AGTAGGAGCT 
GCTGGCAAGG GCTACCTCTG GAAAGCTGCA GGCATGAACA TGGAGGAAGA GGAGGAACTG 
CAGCAGAATC TGTGGGGACT CAAGATCAAC ATGGAAGAAG AGAGTGAAAG TGAAAGTGAG 
CAAAGTATGG ATTCTGAGGA GCCGGACAGC CGGGGAGGCT CCCCTCAGAT GGATGACATC 
AAAGTGTTCC AGAATGAAGT TTTAGGAACA CTACAGCGGG GCAAAGAGGA GAACATTTCT 
TGTGACAATC TCGTCCTGGA AATCAACTCT CTCAAGTATG CCTATAACAT AAGTCTAAAG 
GAGGTGATGC AGGTACTGAG CCACGTGGTC CTGGAGTTCC CCCTGCAACA GATGGATTCC 
CCGCTTGACT CAAGCCGCTA CTGTGCCCTG CTGCTTCCTC TGCTAAAGGC CTGGAGCCCT 
GTTTTTAGGA ACTACATAAA GCGCGCAGCC GACCATTTGG AAGCGTTAGC AGCCATTGAG 
GACTTCTTCC TAGAGCATGA AGCTCTTGGT ATTTCCATGG CCAAGGTACT GATGGCTTTC 
TACCAGCTGG AGATCCTGGC TGAGGAAACA ATTCTGAGCT GGTTCAGCCA AAGAGATACA 
ACTGACAAGG GCCAGCAGTT GCGCAAGAAT CAACAGCTGC AGAGGTTCAT CCAGTGGCTA 
AAAGAGGCAG AAGAGGAGTC ATCTGAAGAT GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62294
Clone ID Related Accession (Same CDS sequence) XM_011513266.3 , XM_011513266.2
Accession Version XM_011513266.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1329bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product translation initiation factor eIF-2B subunit epsilon isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011513266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCTGATCC TAGGGAACCA GATCCACATG CACGTAACAG CTAAGGAATA TGGTGCCCGT 
GTCTCCAACC TACACATGTA CTCAGCTGTC TGTGCTGACG TCATCCGCCG ATGGGTCTAC 
CCTCTCACCC CAGAGGCGAA CTTCACTGAC AGCACCACCC AGAGCTGCAC TCATTCCCGG 
CACAACATCT ACCGAGGGCC TGAGGTCAGC CTGGGCCATG GCAGCATCCT AGAGGAAAAT 
GTGCTCCTGG GCTCTGGCAC TGTCATTGGC AGCAATTGCT TTATCACCAA CAGTGTCATT 
GGCCCCGGCT GCCACATTGG TGATAACGTG GTGCTGGACC AGACCTACCT GTGGCAGGGT 
GTTCGAGTGG CGGCTGGAGC ACAGATCCAT CAGTCTCTGC TTTGTGACAA TGCTGAGGTC 
AAGGAACGAG TGACACTGAA ACCACGCTCT GTCCTCACTT CCCAGGTGGT CGTGGGCCCA 
AATATCACGC TGCCTGAGGG CTCGGTGATC TCTTTGCACC CTCCAGATGC AGAGGAAGAT 
GAAGATGATG GCGAGTTCAG TGATGATTCT GGGGCTGACC AAGAAAAGGA CAAAGTGAAG 
ATGAAAGGTT ACAATCCAGC AGAAGTAGGA GCTGCTGGCA AGGGCTACCT CTGGAAAGCT 
GCAGGCATGA ACATGGAGGA AGAGGAGGAA CTGCAGCAGA ATCTGTGGGG ACTCAAGATC 
AACATGGAAG AAGAGAGTGA AAGTGAAAGT GAGCAAAGTA TGGATTCTGA GGAGCCGGAC 
AGCCGGGGAG GCTCCCCTCA GATGGATGAC ATCAAAGTGT TCCAGAATGA AGTTTTAGGA 
ACACTACAGC GGGGCAAAGA GGAGAACATT TCTTGTGACA ATCTCGTCCT GGAAATCAAC 
TCTCTCAAGT ATGCCTATAA CATAAGTCTA AAGGAGGTGA TGCAGGTACT GAGCCACGTG 
GTCCTGGAGT TCCCCCTGCA ACAGATGGAT TCCCCGCTTG ACTCAAGCCG CTACTGTGCC 
CTGCTGCTTC CTCTGCTAAA GGCCTGGAGC CCTGTTTTTA GGAACTACAT AAAGCGCGCA 
GCCGACCATT TGGAAGCGTT AGCAGCCATT GAGGACTTCT TCCTAGAGCA TGAAGCTCTT 
GGTATTTCCA TGGCCAAGGT ACTGATGGCT TTCTACCAGC TGGAGATCCT GGCTGAGGAA 
ACAATTCTGA GCTGGTTCAG CCAAAGAGAT ACAACTGACA AGGGCCAGCA GTTGCGCAAG 
AATCAACAGC TGCAGAGGTT CATCCAGTGG CTAAAAGAGG CAGAAGAGGA GTCATCTGAA 
GATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511568.1
CDS913..2241
Translation

Target ORF information:

RefSeq Version XM_011513266.3
Organism Homo sapiens(human)
Definition Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011513266.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCTGATCC TAGGGAACCA GATCCACATG CACGTAACAG CTAAGGAATA TGGTGCCCGT 
GTCTCCAACC TACACATGTA CTCAGCTGTC TGTGCTGACG TCATCCGCCG ATGGGTCTAC 
CCTCTCACCC CAGAGGCGAA CTTCACTGAC AGCACCACCC AGAGCTGCAC TCATTCCCGG 
CACAACATCT ACCGAGGGCC TGAGGTCAGC CTGGGCCATG GCAGCATCCT AGAGGAAAAT 
GTGCTCCTGG GCTCTGGCAC TGTCATTGGC AGCAATTGCT TTATCACCAA CAGTGTCATT 
GGCCCCGGCT GCCACATTGG TGATAACGTG GTGCTGGACC AGACCTACCT GTGGCAGGGT 
GTTCGAGTGG CGGCTGGAGC ACAGATCCAT CAGTCTCTGC TTTGTGACAA TGCTGAGGTC 
AAGGAACGAG TGACACTGAA ACCACGCTCT GTCCTCACTT CCCAGGTGGT CGTGGGCCCA 
AATATCACGC TGCCTGAGGG CTCGGTGATC TCTTTGCACC CTCCAGATGC AGAGGAAGAT 
GAAGATGATG GCGAGTTCAG TGATGATTCT GGGGCTGACC AAGAAAAGGA CAAAGTGAAG 
ATGAAAGGTT ACAATCCAGC AGAAGTAGGA GCTGCTGGCA AGGGCTACCT CTGGAAAGCT 
GCAGGCATGA ACATGGAGGA AGAGGAGGAA CTGCAGCAGA ATCTGTGGGG ACTCAAGATC 
AACATGGAAG AAGAGAGTGA AAGTGAAAGT GAGCAAAGTA TGGATTCTGA GGAGCCGGAC 
AGCCGGGGAG GCTCCCCTCA GATGGATGAC ATCAAAGTGT TCCAGAATGA AGTTTTAGGA 
ACACTACAGC GGGGCAAAGA GGAGAACATT TCTTGTGACA ATCTCGTCCT GGAAATCAAC 
TCTCTCAAGT ATGCCTATAA CATAAGTCTA AAGGAGGTGA TGCAGGTACT GAGCCACGTG 
GTCCTGGAGT TCCCCCTGCA ACAGATGGAT TCCCCGCTTG ACTCAAGCCG CTACTGTGCC 
CTGCTGCTTC CTCTGCTAAA GGCCTGGAGC CCTGTTTTTA GGAACTACAT AAAGCGCGCA 
GCCGACCATT TGGAAGCGTT AGCAGCCATT GAGGACTTCT TCCTAGAGCA TGAAGCTCTT 
GGTATTTCCA TGGCCAAGGT ACTGATGGCT TTCTACCAGC TGGAGATCCT GGCTGAGGAA 
ACAATTCTGA GCTGGTTCAG CCAAAGAGAT ACAACTGACA AGGGCCAGCA GTTGCGCAAG 
AATCAACAGC TGCAGAGGTT CATCCAGTGG CTAAAAGAGG CAGAAGAGGA GTCATCTGAA 
GATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62294
Clone ID Related Accession (Same CDS sequence) XM_011513266.3 , XM_011513266.2
Accession Version XM_011513266.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1329bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product translation initiation factor eIF-2B subunit epsilon isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCTGATCC TAGGGAACCA GATCCACATG CACGTAACAG CTAAGGAATA TGGTGCCCGT 
GTCTCCAACC TACACATGTA CTCAGCTGTC TGTGCTGACG TCATCCGCCG ATGGGTCTAC 
CCTCTCACCC CAGAGGCGAA CTTCACTGAC AGCACCACCC AGAGCTGCAC TCATTCCCGG 
CACAACATCT ACCGAGGGCC TGAGGTCAGC CTGGGCCATG GCAGCATCCT AGAGGAAAAT 
GTGCTCCTGG GCTCTGGCAC TGTCATTGGC AGCAATTGCT TTATCACCAA CAGTGTCATT 
GGCCCCGGCT GCCACATTGG TGATAACGTG GTGCTGGACC AGACCTACCT GTGGCAGGGT 
GTTCGAGTGG CGGCTGGAGC ACAGATCCAT CAGTCTCTGC TTTGTGACAA TGCTGAGGTC 
AAGGAACGAG TGACACTGAA ACCACGCTCT GTCCTCACTT CCCAGGTGGT CGTGGGCCCA 
AATATCACGC TGCCTGAGGG CTCGGTGATC TCTTTGCACC CTCCAGATGC AGAGGAAGAT 
GAAGATGATG GCGAGTTCAG TGATGATTCT GGGGCTGACC AAGAAAAGGA CAAAGTGAAG 
ATGAAAGGTT ACAATCCAGC AGAAGTAGGA GCTGCTGGCA AGGGCTACCT CTGGAAAGCT 
GCAGGCATGA ACATGGAGGA AGAGGAGGAA CTGCAGCAGA ATCTGTGGGG ACTCAAGATC 
AACATGGAAG AAGAGAGTGA AAGTGAAAGT GAGCAAAGTA TGGATTCTGA GGAGCCGGAC 
AGCCGGGGAG GCTCCCCTCA GATGGATGAC ATCAAAGTGT TCCAGAATGA AGTTTTAGGA 
ACACTACAGC GGGGCAAAGA GGAGAACATT TCTTGTGACA ATCTCGTCCT GGAAATCAAC 
TCTCTCAAGT ATGCCTATAA CATAAGTCTA AAGGAGGTGA TGCAGGTACT GAGCCACGTG 
GTCCTGGAGT TCCCCCTGCA ACAGATGGAT TCCCCGCTTG ACTCAAGCCG CTACTGTGCC 
CTGCTGCTTC CTCTGCTAAA GGCCTGGAGC CCTGTTTTTA GGAACTACAT AAAGCGCGCA 
GCCGACCATT TGGAAGCGTT AGCAGCCATT GAGGACTTCT TCCTAGAGCA TGAAGCTCTT 
GGTATTTCCA TGGCCAAGGT ACTGATGGCT TTCTACCAGC TGGAGATCCT GGCTGAGGAA 
ACAATTCTGA GCTGGTTCAG CCAAAGAGAT ACAACTGACA AGGGCCAGCA GTTGCGCAAG 
AATCAACAGC TGCAGAGGTT CATCCAGTGG CTAAAAGAGG CAGAAGAGGA GTCATCTGAA 
GATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511568.1
CDS678..2006
Translation

Target ORF information:

RefSeq Version XM_011513266.2
Organism Homo sapiens(human)
Definition Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011513266.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGCTGATCC TAGGGAACCA GATCCACATG CACGTAACAG CTAAGGAATA TGGTGCCCGT 
GTCTCCAACC TACACATGTA CTCAGCTGTC TGTGCTGACG TCATCCGCCG ATGGGTCTAC 
CCTCTCACCC CAGAGGCGAA CTTCACTGAC AGCACCACCC AGAGCTGCAC TCATTCCCGG 
CACAACATCT ACCGAGGGCC TGAGGTCAGC CTGGGCCATG GCAGCATCCT AGAGGAAAAT 
GTGCTCCTGG GCTCTGGCAC TGTCATTGGC AGCAATTGCT TTATCACCAA CAGTGTCATT 
GGCCCCGGCT GCCACATTGG TGATAACGTG GTGCTGGACC AGACCTACCT GTGGCAGGGT 
GTTCGAGTGG CGGCTGGAGC ACAGATCCAT CAGTCTCTGC TTTGTGACAA TGCTGAGGTC 
AAGGAACGAG TGACACTGAA ACCACGCTCT GTCCTCACTT CCCAGGTGGT CGTGGGCCCA 
AATATCACGC TGCCTGAGGG CTCGGTGATC TCTTTGCACC CTCCAGATGC AGAGGAAGAT 
GAAGATGATG GCGAGTTCAG TGATGATTCT GGGGCTGACC AAGAAAAGGA CAAAGTGAAG 
ATGAAAGGTT ACAATCCAGC AGAAGTAGGA GCTGCTGGCA AGGGCTACCT CTGGAAAGCT 
GCAGGCATGA ACATGGAGGA AGAGGAGGAA CTGCAGCAGA ATCTGTGGGG ACTCAAGATC 
AACATGGAAG AAGAGAGTGA AAGTGAAAGT GAGCAAAGTA TGGATTCTGA GGAGCCGGAC 
AGCCGGGGAG GCTCCCCTCA GATGGATGAC ATCAAAGTGT TCCAGAATGA AGTTTTAGGA 
ACACTACAGC GGGGCAAAGA GGAGAACATT TCTTGTGACA ATCTCGTCCT GGAAATCAAC 
TCTCTCAAGT ATGCCTATAA CATAAGTCTA AAGGAGGTGA TGCAGGTACT GAGCCACGTG 
GTCCTGGAGT TCCCCCTGCA ACAGATGGAT TCCCCGCTTG ACTCAAGCCG CTACTGTGCC 
CTGCTGCTTC CTCTGCTAAA GGCCTGGAGC CCTGTTTTTA GGAACTACAT AAAGCGCGCA 
GCCGACCATT TGGAAGCGTT AGCAGCCATT GAGGACTTCT TCCTAGAGCA TGAAGCTCTT 
GGTATTTCCA TGGCCAAGGT ACTGATGGCT TTCTACCAGC TGGAGATCCT GGCTGAGGAA 
ACAATTCTGA GCTGGTTCAG CCAAAGAGAT ACAACTGACA AGGGCCAGCA GTTGCGCAAG 
AATCAACAGC TGCAGAGGTT CATCCAGTGG CTAAAAGAGG CAGAAGAGGA GTCATCTGAA 
GATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21417
Clone ID Related Accession (Same CDS sequence) NM_003907.2 , NM_003907.3
Accession Version NM_003907.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-11-11
Organism Homo sapiens(human)
Product translation initiation factor eIF-2B subunit epsilon
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BI769147.1, BC013590.1 and AC131235.4. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2005 this sequence version replaced NM_003907.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013590.1, SRR1803616.131751.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCC CTGTAGTGGC GCCGCCTGGT GTGGTGGTTA GTCGGGCTAA CAAGCGCAGC 
GGCGCGGGGC CGGGAGGCAG CGGTGGCGGG GGAGCCAGAG GGGCGGAGGA GGAACCGCCG 
CCGCCCCTAC AAGCAGTTCT GGTGGCCGAT AGCTTCGATC GCCGCTTCTT CCCCATCTCC 
AAGGACCAGC CTCGGGTCCT CTTGCCCCTG GCCAATGTGG CATTAATTGA CTACACTCTG 
GAATTCCTGA CTGCCACAGG TGTACAGGAA ACATTTGTCT TTTGTTGCTG GAAAGCTGCT 
CAAATCAAAG AACATTTACT GAAGTCAAAG TGGTGCCGCC CTACATCTCT CAATGTGGTT 
CGAATAATTA CATCAGAGCT CTATCGATCA CTGGGAGATG TCCTCCGTGA TGTTGATGCC 
AAGGCTTTGG TGCGCTCTGA CTTTCTTCTG GTGTATGGGG ATGTCATCTC AAACATCAAT 
ATCACCAGAG CCCTTGAGGA ACACAGGTTG AGACGGAAGC TAGAAAAAAA TGTTTCTGTG 
ATGACGATGA TCTTCAAGGA GTCATCCCCC AGCCACCCAA CTCGTTGCCA CGAAGACAAT 
GTGGTAGTGG CTGTGGATAG TACCACAAAC AGGGTTCTCC ATTTTCAGAA GACCCAGGGT 
CTCCGGCGTT TTGCATTTCC TCTGAGCCTG TTTCAGGGCA GTAGTGATGG AGTGGAGGTT 
CGATATGATT TACTGGATTG TCATATCAGC ATCTGTTCTC CTCAGGTGGC ACAACTCTTT 
ACAGACAACT TTGACTACCA AACTCGAGAT GACTTTGTGC GAGGTCTCTT AGTGAATGAG 
GAGATCCTAG GGAACCAGAT CCACATGCAC GTAACAGCTA AGGAATATGG TGCCCGTGTC 
TCCAACCTAC ACATGTACTC AGCTGTCTGT GCTGACGTCA TCCGCCGATG GGTCTACCCT 
CTCACCCCAG AGGCGAACTT CACTGACAGC ACCACCCAGA GCTGCACTCA TTCCCGGCAC 
AACATCTACC GAGGGCCTGA GGTCAGCCTG GGCCATGGCA GCATCCTAGA GGAAAATGTG 
CTCCTGGGCT CTGGCACTGT CATTGGCAGC AATTGCTTTA TCACCAACAG TGTCATTGGC 
CCCGGCTGCC ACATTGGTGA TAACGTGGTG CTGGACCAGA CCTACCTGTG GCAGGGTGTT 
CGAGTGGCGG CTGGAGCACA GATCCATCAG TCTCTGCTTT GTGACAATGC TGAGGTCAAG 
GAACGAGTGA CACTGAAACC ACGCTCTGTC CTCACTTCCC AGGTGGTCGT GGGCCCAAAT 
ATCACGCTGC CTGAGGGCTC GGTGATCTCT TTGCACCCTC CAGATGCAGA GGAAGATGAA 
GATGATGGCG AGTTCAGTGA TGATTCTGGG GCTGACCAAG AAAAGGACAA AGTGAAGATG 
AAAGGTTACA ATCCAGCAGA AGTAGGAGCT GCTGGCAAGG GCTACCTCTG GAAAGCTGCA 
GGCATGAACA TGGAGGAAGA GGAGGAACTG CAGCAGAATC TGTGGGGACT CAAGATCAAC 
ATGGAAGAAG AGAGTGAAAG TGAAAGTGAG CAAAGTATGG ATTCTGAGGA GCCGGACAGC 
CGGGGAGGCT CCCCTCAGAT GGATGACATC AAAGTGTTCC AGAATGAAGT TTTAGGAACA 
CTACAGCGGG GCAAAGAGGA GAACATTTCT TGTGACAATC TCGTCCTGGA AATCAACTCT 
CTCAAGTATG CCTATAACAT AAGTCTAAAG GAGGTGATGC AGGTACTGAG CCACGTGGTC 
CTGGAGTTCC CCCTGCAACA GATGGATTCC CCGCTTGACT CAAGCCGCTA CTGTGCCCTG 
CTGCTTCCTC TGCTAAAGGC CTGGAGCCCT GTTTTTAGGA ACTACATAAA GCGCGCAGCC 
GACCATTTGG AAGCGTTAGC AGCCATTGAG GACTTCTTCC TAGAGCATGA AGCTCTTGGT 
ATTTCCATGG CCAAGGTACT GATGGCTTTC TACCAGCTGG AGATCCTGGC TGAGGAAACA 
ATTCTGAGCT GGTTCAGCCA AAGAGATACA ACTGACAAGG GCCAGCAGTT GCGCAAGAAT 
CAACAGCTGC AGAGGTTCAT CCAGTGGCTA AAAGAGGCAG AAGAGGAGTC ATCTGAAGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003898.2
CDS365..2530
Translation

Target ORF information:

RefSeq Version NM_003907.2
Organism Homo sapiens(human)
Definition Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003907.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCC CTGTAGTGGC GCCGCCTGGT GTGGTGGTTA GTCGGGCTAA CAAGCGCAGC 
GGCGCGGGGC CGGGAGGCAG CGGTGGCGGG GGAGCCAGAG GGGCGGAGGA GGAACCGCCG 
CCGCCCCTAC AAGCAGTTCT GGTGGCCGAT AGCTTCGATC GCCGCTTCTT CCCCATCTCC 
AAGGACCAGC CTCGGGTCCT CTTGCCCCTG GCCAATGTGG CATTAATTGA CTACACTCTG 
GAATTCCTGA CTGCCACAGG TGTACAGGAA ACATTTGTCT TTTGTTGCTG GAAAGCTGCT 
CAAATCAAAG AACATTTACT GAAGTCAAAG TGGTGCCGCC CTACATCTCT CAATGTGGTT 
CGAATAATTA CATCAGAGCT CTATCGATCA CTGGGAGATG TCCTCCGTGA TGTTGATGCC 
AAGGCTTTGG TGCGCTCTGA CTTTCTTCTG GTGTATGGGG ATGTCATCTC AAACATCAAT 
ATCACCAGAG CCCTTGAGGA ACACAGGTTG AGACGGAAGC TAGAAAAAAA TGTTTCTGTG 
ATGACGATGA TCTTCAAGGA GTCATCCCCC AGCCACCCAA CTCGTTGCCA CGAAGACAAT 
GTGGTAGTGG CTGTGGATAG TACCACAAAC AGGGTTCTCC ATTTTCAGAA GACCCAGGGT 
CTCCGGCGTT TTGCATTTCC TCTGAGCCTG TTTCAGGGCA GTAGTGATGG AGTGGAGGTT 
CGATATGATT TACTGGATTG TCATATCAGC ATCTGTTCTC CTCAGGTGGC ACAACTCTTT 
ACAGACAACT TTGACTACCA AACTCGAGAT GACTTTGTGC GAGGTCTCTT AGTGAATGAG 
GAGATCCTAG GGAACCAGAT CCACATGCAC GTAACAGCTA AGGAATATGG TGCCCGTGTC 
TCCAACCTAC ACATGTACTC AGCTGTCTGT GCTGACGTCA TCCGCCGATG GGTCTACCCT 
CTCACCCCAG AGGCGAACTT CACTGACAGC ACCACCCAGA GCTGCACTCA TTCCCGGCAC 
AACATCTACC GAGGGCCTGA GGTCAGCCTG GGCCATGGCA GCATCCTAGA GGAAAATGTG 
CTCCTGGGCT CTGGCACTGT CATTGGCAGC AATTGCTTTA TCACCAACAG TGTCATTGGC 
CCCGGCTGCC ACATTGGTGA TAACGTGGTG CTGGACCAGA CCTACCTGTG GCAGGGTGTT 
CGAGTGGCGG CTGGAGCACA GATCCATCAG TCTCTGCTTT GTGACAATGC TGAGGTCAAG 
GAACGAGTGA CACTGAAACC ACGCTCTGTC CTCACTTCCC AGGTGGTCGT GGGCCCAAAT 
ATCACGCTGC CTGAGGGCTC GGTGATCTCT TTGCACCCTC CAGATGCAGA GGAAGATGAA 
GATGATGGCG AGTTCAGTGA TGATTCTGGG GCTGACCAAG AAAAGGACAA AGTGAAGATG 
AAAGGTTACA ATCCAGCAGA AGTAGGAGCT GCTGGCAAGG GCTACCTCTG GAAAGCTGCA 
GGCATGAACA TGGAGGAAGA GGAGGAACTG CAGCAGAATC TGTGGGGACT CAAGATCAAC 
ATGGAAGAAG AGAGTGAAAG TGAAAGTGAG CAAAGTATGG ATTCTGAGGA GCCGGACAGC 
CGGGGAGGCT CCCCTCAGAT GGATGACATC AAAGTGTTCC AGAATGAAGT TTTAGGAACA 
CTACAGCGGG GCAAAGAGGA GAACATTTCT TGTGACAATC TCGTCCTGGA AATCAACTCT 
CTCAAGTATG CCTATAACAT AAGTCTAAAG GAGGTGATGC AGGTACTGAG CCACGTGGTC 
CTGGAGTTCC CCCTGCAACA GATGGATTCC CCGCTTGACT CAAGCCGCTA CTGTGCCCTG 
CTGCTTCCTC TGCTAAAGGC CTGGAGCCCT GTTTTTAGGA ACTACATAAA GCGCGCAGCC 
GACCATTTGG AAGCGTTAGC AGCCATTGAG GACTTCTTCC TAGAGCATGA AGCTCTTGGT 
ATTTCCATGG CCAAGGTACT GATGGCTTTC TACCAGCTGG AGATCCTGGC TGAGGAAACA 
ATTCTGAGCT GGTTCAGCCA AAGAGATACA ACTGACAAGG GCCAGCAGTT GCGCAAGAAT 
CAACAGCTGC AGAGGTTCAT CCAGTGGCTA AAAGAGGCAG AAGAGGAGTC ATCTGAAGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21417
Clone ID Related Accession (Same CDS sequence) NM_003907.2 , NM_003907.3
Accession Version NM_003907.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2166bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-14
Organism Homo sapiens(human)
Product translation initiation factor eIF-2B subunit epsilon
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BC013590.1 and AC131235.4. This sequence is a reference standard in the RefSeqGene project. On Aug 31, 2018 this sequence version replaced NM_003907.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.231381.1, SRR1803612.95745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000648915.2/ ENSP00000497160.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCC CTGTAGTGGC GCCGCCTGGT GTGGTGGTTA GTCGGGCTAA CAAGCGCAGC 
GGCGCGGGGC CGGGAGGCAG CGGTGGCGGG GGAGCCAGAG GGGCGGAGGA GGAACCGCCG 
CCGCCCCTAC AAGCAGTTCT GGTGGCCGAT AGCTTCGATC GCCGCTTCTT CCCCATCTCC 
AAGGACCAGC CTCGGGTCCT CTTGCCCCTG GCCAATGTGG CATTAATTGA CTACACTCTG 
GAATTCCTGA CTGCCACAGG TGTACAGGAA ACATTTGTCT TTTGTTGCTG GAAAGCTGCT 
CAAATCAAAG AACATTTACT GAAGTCAAAG TGGTGCCGCC CTACATCTCT CAATGTGGTT 
CGAATAATTA CATCAGAGCT CTATCGATCA CTGGGAGATG TCCTCCGTGA TGTTGATGCC 
AAGGCTTTGG TGCGCTCTGA CTTTCTTCTG GTGTATGGGG ATGTCATCTC AAACATCAAT 
ATCACCAGAG CCCTTGAGGA ACACAGGTTG AGACGGAAGC TAGAAAAAAA TGTTTCTGTG 
ATGACGATGA TCTTCAAGGA GTCATCCCCC AGCCACCCAA CTCGTTGCCA CGAAGACAAT 
GTGGTAGTGG CTGTGGATAG TACCACAAAC AGGGTTCTCC ATTTTCAGAA GACCCAGGGT 
CTCCGGCGTT TTGCATTTCC TCTGAGCCTG TTTCAGGGCA GTAGTGATGG AGTGGAGGTT 
CGATATGATT TACTGGATTG TCATATCAGC ATCTGTTCTC CTCAGGTGGC ACAACTCTTT 
ACAGACAACT TTGACTACCA AACTCGAGAT GACTTTGTGC GAGGTCTCTT AGTGAATGAG 
GAGATCCTAG GGAACCAGAT CCACATGCAC GTAACAGCTA AGGAATATGG TGCCCGTGTC 
TCCAACCTAC ACATGTACTC AGCTGTCTGT GCTGACGTCA TCCGCCGATG GGTCTACCCT 
CTCACCCCAG AGGCGAACTT CACTGACAGC ACCACCCAGA GCTGCACTCA TTCCCGGCAC 
AACATCTACC GAGGGCCTGA GGTCAGCCTG GGCCATGGCA GCATCCTAGA GGAAAATGTG 
CTCCTGGGCT CTGGCACTGT CATTGGCAGC AATTGCTTTA TCACCAACAG TGTCATTGGC 
CCCGGCTGCC ACATTGGTGA TAACGTGGTG CTGGACCAGA CCTACCTGTG GCAGGGTGTT 
CGAGTGGCGG CTGGAGCACA GATCCATCAG TCTCTGCTTT GTGACAATGC TGAGGTCAAG 
GAACGAGTGA CACTGAAACC ACGCTCTGTC CTCACTTCCC AGGTGGTCGT GGGCCCAAAT 
ATCACGCTGC CTGAGGGCTC GGTGATCTCT TTGCACCCTC CAGATGCAGA GGAAGATGAA 
GATGATGGCG AGTTCAGTGA TGATTCTGGG GCTGACCAAG AAAAGGACAA AGTGAAGATG 
AAAGGTTACA ATCCAGCAGA AGTAGGAGCT GCTGGCAAGG GCTACCTCTG GAAAGCTGCA 
GGCATGAACA TGGAGGAAGA GGAGGAACTG CAGCAGAATC TGTGGGGACT CAAGATCAAC 
ATGGAAGAAG AGAGTGAAAG TGAAAGTGAG CAAAGTATGG ATTCTGAGGA GCCGGACAGC 
CGGGGAGGCT CCCCTCAGAT GGATGACATC AAAGTGTTCC AGAATGAAGT TTTAGGAACA 
CTACAGCGGG GCAAAGAGGA GAACATTTCT TGTGACAATC TCGTCCTGGA AATCAACTCT 
CTCAAGTATG CCTATAACAT AAGTCTAAAG GAGGTGATGC AGGTACTGAG CCACGTGGTC 
CTGGAGTTCC CCCTGCAACA GATGGATTCC CCGCTTGACT CAAGCCGCTA CTGTGCCCTG 
CTGCTTCCTC TGCTAAAGGC CTGGAGCCCT GTTTTTAGGA ACTACATAAA GCGCGCAGCC 
GACCATTTGG AAGCGTTAGC AGCCATTGAG GACTTCTTCC TAGAGCATGA AGCTCTTGGT 
ATTTCCATGG CCAAGGTACT GATGGCTTTC TACCAGCTGG AGATCCTGGC TGAGGAAACA 
ATTCTGAGCT GGTTCAGCCA AAGAGATACA ACTGACAAGG GCCAGCAGTT GCGCAAGAAT 
CAACAGCTGC AGAGGTTCAT CCAGTGGCTA AAAGAGGCAG AAGAGGAGTC ATCTGAAGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003898.2
CDS29..2194
Translation

Target ORF information:

RefSeq Version NM_003907.3
Organism Homo sapiens(human)
Definition Homo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_003907.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCGGCCC CTGTAGTGGC GCCGCCTGGT GTGGTGGTTA GTCGGGCTAA CAAGCGCAGC 
GGCGCGGGGC CGGGAGGCAG CGGTGGCGGG GGAGCCAGAG GGGCGGAGGA GGAACCGCCG 
CCGCCCCTAC AAGCAGTTCT GGTGGCCGAT AGCTTCGATC GCCGCTTCTT CCCCATCTCC 
AAGGACCAGC CTCGGGTCCT CTTGCCCCTG GCCAATGTGG CATTAATTGA CTACACTCTG 
GAATTCCTGA CTGCCACAGG TGTACAGGAA ACATTTGTCT TTTGTTGCTG GAAAGCTGCT 
CAAATCAAAG AACATTTACT GAAGTCAAAG TGGTGCCGCC CTACATCTCT CAATGTGGTT 
CGAATAATTA CATCAGAGCT CTATCGATCA CTGGGAGATG TCCTCCGTGA TGTTGATGCC 
AAGGCTTTGG TGCGCTCTGA CTTTCTTCTG GTGTATGGGG ATGTCATCTC AAACATCAAT 
ATCACCAGAG CCCTTGAGGA ACACAGGTTG AGACGGAAGC TAGAAAAAAA TGTTTCTGTG 
ATGACGATGA TCTTCAAGGA GTCATCCCCC AGCCACCCAA CTCGTTGCCA CGAAGACAAT 
GTGGTAGTGG CTGTGGATAG TACCACAAAC AGGGTTCTCC ATTTTCAGAA GACCCAGGGT 
CTCCGGCGTT TTGCATTTCC TCTGAGCCTG TTTCAGGGCA GTAGTGATGG AGTGGAGGTT 
CGATATGATT TACTGGATTG TCATATCAGC ATCTGTTCTC CTCAGGTGGC ACAACTCTTT 
ACAGACAACT TTGACTACCA AACTCGAGAT GACTTTGTGC GAGGTCTCTT AGTGAATGAG 
GAGATCCTAG GGAACCAGAT CCACATGCAC GTAACAGCTA AGGAATATGG TGCCCGTGTC 
TCCAACCTAC ACATGTACTC AGCTGTCTGT GCTGACGTCA TCCGCCGATG GGTCTACCCT 
CTCACCCCAG AGGCGAACTT CACTGACAGC ACCACCCAGA GCTGCACTCA TTCCCGGCAC 
AACATCTACC GAGGGCCTGA GGTCAGCCTG GGCCATGGCA GCATCCTAGA GGAAAATGTG 
CTCCTGGGCT CTGGCACTGT CATTGGCAGC AATTGCTTTA TCACCAACAG TGTCATTGGC 
CCCGGCTGCC ACATTGGTGA TAACGTGGTG CTGGACCAGA CCTACCTGTG GCAGGGTGTT 
CGAGTGGCGG CTGGAGCACA GATCCATCAG TCTCTGCTTT GTGACAATGC TGAGGTCAAG 
GAACGAGTGA CACTGAAACC ACGCTCTGTC CTCACTTCCC AGGTGGTCGT GGGCCCAAAT 
ATCACGCTGC CTGAGGGCTC GGTGATCTCT TTGCACCCTC CAGATGCAGA GGAAGATGAA 
GATGATGGCG AGTTCAGTGA TGATTCTGGG GCTGACCAAG AAAAGGACAA AGTGAAGATG 
AAAGGTTACA ATCCAGCAGA AGTAGGAGCT GCTGGCAAGG GCTACCTCTG GAAAGCTGCA 
GGCATGAACA TGGAGGAAGA GGAGGAACTG CAGCAGAATC TGTGGGGACT CAAGATCAAC 
ATGGAAGAAG AGAGTGAAAG TGAAAGTGAG CAAAGTATGG ATTCTGAGGA GCCGGACAGC 
CGGGGAGGCT CCCCTCAGAT GGATGACATC AAAGTGTTCC AGAATGAAGT TTTAGGAACA 
CTACAGCGGG GCAAAGAGGA GAACATTTCT TGTGACAATC TCGTCCTGGA AATCAACTCT 
CTCAAGTATG CCTATAACAT AAGTCTAAAG GAGGTGATGC AGGTACTGAG CCACGTGGTC 
CTGGAGTTCC CCCTGCAACA GATGGATTCC CCGCTTGACT CAAGCCGCTA CTGTGCCCTG 
CTGCTTCCTC TGCTAAAGGC CTGGAGCCCT GTTTTTAGGA ACTACATAAA GCGCGCAGCC 
GACCATTTGG AAGCGTTAGC AGCCATTGAG GACTTCTTCC TAGAGCATGA AGCTCTTGGT 
ATTTCCATGG CCAAGGTACT GATGGCTTTC TACCAGCTGG AGATCCTGGC TGAGGAAACA 
ATTCTGAGCT GGTTCAGCCA AAGAGATACA ACTGACAAGG GCCAGCAGTT GCGCAAGAAT 
CAACAGCTGC AGAGGTTCAT CCAGTGGCTA AAAGAGGCAG AAGAGGAGTC ATCTGAAGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Transcriptome analysis of hypoxic cancer cells uncovers intron retention in EIF2B5 as a mechanism to inhibit translation.
PLoS biology15(9)e2002623(2017 Sep)
Brady LK,Wang H,Radens CM,Bi Y,Radovich M,Maity A,Ivan C,Ivan M,Barash Y,Koumenis C


book

No evidence for a role of Ile587Val polymorphism of EIF2B5 gene in multiple sclerosis in Kashmir Valley of India.
Journal of the neurological sciences359(1-2)172-6(2015 Dec)
Zahoor I,Asimi R,Haq E


book

A Japanese girl with an early-infantile onset vanishing white matter disease resembling Cree leukoencephalopathy.
Brain & development37(6)638-42(2015 Jun)
Takano K,Tsuyusaki Y,Sato M,Takagi M,Anzai R,Okuda M,Iai M,Yamashita S,Okabe T,Aida N,Tsurusaki Y,Saitsu H,Matsumoto N,Osaka H


book

Novel mutations identified in EIF2B5 gene in Kashmiri patients as susceptibility factor for multiple sclerosis.
Indian journal of biochemistry & biophysics51(2)115-20(2014 Apr)
Zahoor I,Hamid Z,Asimi R,Haq E