TMEM259 cDNA ORF clone, Homo sapiens(human)

The following TMEM259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu104775 XM_024451773.1
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu13093 NM_033420.4
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
OHu13102 NM_001033026.2
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51890 XM_005259675.3
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51891 XM_005259676.3
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51892 XM_005259677.3
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51893 XM_005259678.3
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu88807 XM_017027457.2
Latest version!
Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
View Old Accession Versions >>
OHu51890 XM_005259675.2 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51892 XM_005259677.2 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu88807 XM_017027457.1 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51891 XM_005259676.2 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu51893 XM_005259678.2 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu13102 NM_001033026.1 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu13093 NM_033420.3 Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu104775
    Clone ID Related Accession (Same CDS sequence) XM_024451773.1
    Accession Version XM_024451773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product membralin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG CGCCCCTGCT GACCGTCATC
    CTGGCCCTCG TCGGGATGGA GGCCATCATG TCGGAGTTCT TCAACGACAC CACCACCGCC
    TTCTACATCA TCCTCATCGT GTGGCTCGCG GACCAGTATG ACGCCATCTG CTGCCACACC
    AGCACCAGCA AGCGGCATTG GCTGCGGTTC TTCTATCTCT ACCACTTCGC CTTCTATGCC
    TATCACTACC GCTTCAATGG GCAGTATAGC AGCCTGGCCC TGGTCACCTC CTGGCTCTTC
    ATCCAGCATT CCATGATCTA CTTCTTCCAC CACTACGAGC TGCCTGCCAT CCTGCAGCAG
    GTCCGCATCC AGGAGATGCT GCTTCAGGCG CCGCCACTGG GCCCCGGGAC CCCCACGGCG
    CTGCCCGATG ACATGAACAA CAACTCGGGC GCCCCGGCTA CAGCCCCTGA CTCTGCCGGC
    CAGCCCCCCG CCCTGGGCCC CGTCTCGCCT GGGGCCAGCG GGAGTCCCGG GCCTGTGGCA
    GCGGCGCCCA GCTCCCTGGT GGCCGCGGCA GCCTCAGTGG CAGCAGCTGC CGGTGGTGAC
    CTGGGTTGGA TGGCAGAGAC CGCTGCCATC ATCACAGACG CCTCCTTCCT GTCCGGCCTG
    AGCGCCTCCC TCCTGGAGCG GCGTCCAGCC AGCCCGCTGG GCCCTGCTGG GGGCCTCCCC
    CACGCCCCCC AGGACAGTGT CCCCCCGAGT GACTCCGCAG CTTCTGACAC AACTCCCCTG
    GGGGCTGCGG TAGGCGGGCC TAGCCCGGCC TCCATGGCCC CAACGGAGGC GCCCTCGGAG
    GTGGGGTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024307541.1
    CDS147..1958
    Translation

    Target ORF information:

    RefSeq Version XM_024451773.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024451773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG CGCCCCTGCT GACCGTCATC
    CTGGCCCTCG TCGGGATGGA GGCCATCATG TCGGAGTTCT TCAACGACAC CACCACCGCC
    TTCTACATCA TCCTCATCGT GTGGCTCGCG GACCAGTATG ACGCCATCTG CTGCCACACC
    AGCACCAGCA AGCGGCATTG GCTGCGGTTC TTCTATCTCT ACCACTTCGC CTTCTATGCC
    TATCACTACC GCTTCAATGG GCAGTATAGC AGCCTGGCCC TGGTCACCTC CTGGCTCTTC
    ATCCAGCATT CCATGATCTA CTTCTTCCAC CACTACGAGC TGCCTGCCAT CCTGCAGCAG
    GTCCGCATCC AGGAGATGCT GCTTCAGGCG CCGCCACTGG GCCCCGGGAC CCCCACGGCG
    CTGCCCGATG ACATGAACAA CAACTCGGGC GCCCCGGCTA CAGCCCCTGA CTCTGCCGGC
    CAGCCCCCCG CCCTGGGCCC CGTCTCGCCT GGGGCCAGCG GGAGTCCCGG GCCTGTGGCA
    GCGGCGCCCA GCTCCCTGGT GGCCGCGGCA GCCTCAGTGG CAGCAGCTGC CGGTGGTGAC
    CTGGGTTGGA TGGCAGAGAC CGCTGCCATC ATCACAGACG CCTCCTTCCT GTCCGGCCTG
    AGCGCCTCCC TCCTGGAGCG GCGTCCAGCC AGCCCGCTGG GCCCTGCTGG GGGCCTCCCC
    CACGCCCCCC AGGACAGTGT CCCCCCGAGT GACTCCGCAG CTTCTGACAC AACTCCCCTG
    GGGGCTGCGG TAGGCGGGCC TAGCCCGGCC TCCATGGCCC CAACGGAGGC GCCCTCGGAG
    GTGGGGTCCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13093
    Clone ID Related Accession (Same CDS sequence) NM_033420.3 , NM_033420.4
    Accession Version NM_033420.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product membralin isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK095756.1, BQ895919.1, BC008957.2 and AK090400.1. On or before Mar 29, 2008 this sequence version replaced XR_017883.2, NM_033420.2. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC008957.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGCAT TCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_219488.1
    CDS147..1373
    Translation

    Target ORF information:

    RefSeq Version NM_033420.3
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033420.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGCAT TCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13093
    Clone ID Related Accession (Same CDS sequence) NM_033420.3 , NM_033420.4
    Accession Version NM_033420.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product membralin isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK095756.1, BQ895919.1, BC008957.2 and AK090400.1. On May 31, 2019 this sequence version replaced NM_033420.3. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC008957.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGCAT TCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_219488.1
    CDS128..1354
    Translation

    Target ORF information:

    RefSeq Version NM_033420.4
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_033420.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGCAT TCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13102
    Clone ID Related Accession (Same CDS sequence) NM_001033026.1 , NM_001033026.2
    Accession Version NM_001033026.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-09
    Organism Homo sapiens(human)
    Product membralin isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK095756.1, BQ895919.1, DQ005958.1 and AK090400.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: DQ005958.1, SRR1163658.550455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGCAT
    TCCATGATCT ACTTCTTCCA CCACTACGAG CTGCCTGCCA TCCTGCAGCA GGTCCGCATC
    CAGGAGATGC TGCTTCAGGC GCCGCCACTG GGCCCCGGGA CCCCCACGGC GCTGCCCGAT
    GACATGAACA ACAACTCGGG CGCCCCGGCT ACAGCCCCTG ACTCTGCCGG CCAGCCCCCC
    GCCCTGGGCC CCGTCTCGCC TGGGGCCAGC GGGAGTCCCG GGCCTGTGGC AGCGGCGCCC
    AGCTCCCTGG TGGCCGCGGC AGCCTCAGTG GCAGCAGCTG CCGGTGGTGA CCTGGGTTGG
    ATGGCAGAGA CCGCTGCCAT CATCACAGAC GCCTCCTTCC TGTCCGGCCT GAGCGCCTCC
    CTCCTGGAGC GGCGTCCAGC CAGCCCGCTG GGCCCTGCTG GGGGCCTCCC CCACGCCCCC
    CAGGACAGTG TCCCCCCGAG TGACTCCGCA GCTTCTGACA CAACTCCCCT GGGGGCTGCG
    GTAGGCGGGC CTAGCCCGGC CTCCATGGCC CCAACGGAGG CGCCCTCGGA GGTGGGGTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001028198.1
    CDS147..2009
    Translation

    Target ORF information:

    RefSeq Version NM_001033026.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001033026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGCAT
    TCCATGATCT ACTTCTTCCA CCACTACGAG CTGCCTGCCA TCCTGCAGCA GGTCCGCATC
    CAGGAGATGC TGCTTCAGGC GCCGCCACTG GGCCCCGGGA CCCCCACGGC GCTGCCCGAT
    GACATGAACA ACAACTCGGG CGCCCCGGCT ACAGCCCCTG ACTCTGCCGG CCAGCCCCCC
    GCCCTGGGCC CCGTCTCGCC TGGGGCCAGC GGGAGTCCCG GGCCTGTGGC AGCGGCGCCC
    AGCTCCCTGG TGGCCGCGGC AGCCTCAGTG GCAGCAGCTG CCGGTGGTGA CCTGGGTTGG
    ATGGCAGAGA CCGCTGCCAT CATCACAGAC GCCTCCTTCC TGTCCGGCCT GAGCGCCTCC
    CTCCTGGAGC GGCGTCCAGC CAGCCCGCTG GGCCCTGCTG GGGGCCTCCC CCACGCCCCC
    CAGGACAGTG TCCCCCCGAG TGACTCCGCA GCTTCTGACA CAACTCCCCT GGGGGCTGCG
    GTAGGCGGGC CTAGCCCGGC CTCCATGGCC CCAACGGAGG CGCCCTCGGA GGTGGGGTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13102
    Clone ID Related Accession (Same CDS sequence) NM_001033026.1 , NM_001033026.2
    Accession Version NM_001033026.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product membralin isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK095756.1, BQ895919.1, DQ005958.1 and AK090400.1. On Nov 23, 2018 this sequence version replaced NM_001033026.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163658.550455.1, DQ005958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356663.8/ ENSP00000349087.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGCAT
    TCCATGATCT ACTTCTTCCA CCACTACGAG CTGCCTGCCA TCCTGCAGCA GGTCCGCATC
    CAGGAGATGC TGCTTCAGGC GCCGCCACTG GGCCCCGGGA CCCCCACGGC GCTGCCCGAT
    GACATGAACA ACAACTCGGG CGCCCCGGCT ACAGCCCCTG ACTCTGCCGG CCAGCCCCCC
    GCCCTGGGCC CCGTCTCGCC TGGGGCCAGC GGGAGTCCCG GGCCTGTGGC AGCGGCGCCC
    AGCTCCCTGG TGGCCGCGGC AGCCTCAGTG GCAGCAGCTG CCGGTGGTGA CCTGGGTTGG
    ATGGCAGAGA CCGCTGCCAT CATCACAGAC GCCTCCTTCC TGTCCGGCCT GAGCGCCTCC
    CTCCTGGAGC GGCGTCCAGC CAGCCCGCTG GGCCCTGCTG GGGGCCTCCC CCACGCCCCC
    CAGGACAGTG TCCCCCCGAG TGACTCCGCA GCTTCTGACA CAACTCCCCT GGGGGCTGCG
    GTAGGCGGGC CTAGCCCGGC CTCCATGGCC CCAACGGAGG CGCCCTCGGA GGTGGGGTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001028198.1
    CDS128..1990
    Translation

    Target ORF information:

    RefSeq Version NM_001033026.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001033026.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGCAT
    TCCATGATCT ACTTCTTCCA CCACTACGAG CTGCCTGCCA TCCTGCAGCA GGTCCGCATC
    CAGGAGATGC TGCTTCAGGC GCCGCCACTG GGCCCCGGGA CCCCCACGGC GCTGCCCGAT
    GACATGAACA ACAACTCGGG CGCCCCGGCT ACAGCCCCTG ACTCTGCCGG CCAGCCCCCC
    GCCCTGGGCC CCGTCTCGCC TGGGGCCAGC GGGAGTCCCG GGCCTGTGGC AGCGGCGCCC
    AGCTCCCTGG TGGCCGCGGC AGCCTCAGTG GCAGCAGCTG CCGGTGGTGA CCTGGGTTGG
    ATGGCAGAGA CCGCTGCCAT CATCACAGAC GCCTCCTTCC TGTCCGGCCT GAGCGCCTCC
    CTCCTGGAGC GGCGTCCAGC CAGCCCGCTG GGCCCTGCTG GGGGCCTCCC CCACGCCCCC
    CAGGACAGTG TCCCCCCGAG TGACTCCGCA GCTTCTGACA CAACTCCCCT GGGGGCTGCG
    GTAGGCGGGC CTAGCCCGGC CTCCATGGCC CCAACGGAGG CGCCCTCGGA GGTGGGGTCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51890
    Clone ID Related Accession (Same CDS sequence) XM_005259675.2 , XM_005259675.3
    Accession Version XM_005259675.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product membralin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005259675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGGTGAG GCCTGGGCGG CAAGCAGGGG GCAGGCCAGC CGTGCCTTTC
    CAGGCAGGAG AGGCTGCAGC CGGGGAGGAT GCCCTGTGGG GTCGGCCCAA GCGGGCAGAG
    CATTCCATGA TCTACTTCTT CCACCACTAC GAGCTGCCTG CCATCCTGCA GCAGGTCCGC
    ATCCAGGAGA TGCTGCTTCA GGCGCCGCCA CTGGGCCCCG GGACCCCCAC GGCGCTGCCC
    GATGACATGA ACAACAACTC GGGCGCCCCG GCTACAGCCC CTGACTCTGC CGGCCAGCCC
    CCCGCCCTGG GCCCCGTCTC GCCTGGGGCC AGCGGGAGTC CCGGGCCTGT GGCAGCGGCG
    CCCAGCTCCC TGGTGGCCGC GGCAGCCTCA GTGGCAGCAG CTGCCGGTGG TGACCTGGGT
    TGGATGGCAG AGACCGCTGC CATCATCACA GACGCCTCCT TCCTGTCCGG CCTGAGCGCC
    TCCCTCCTGG AGCGGCGTCC AGCCAGCCCG CTGGGCCCTG CTGGGGGCCT CCCCCACGCC
    CCCCAGGACA GTGTCCCCCC GAGTGACTCC GCAGCTTCTG ACACAACTCC CCTGGGGGCT
    GCGGTAGGCG GGCCTAGCCC GGCCTCCATG GCCCCAACGG AGGCGCCCTC GGAGGTGGGG
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259732.1
    CDS174..2159
    Translation

    Target ORF information:

    RefSeq Version XM_005259675.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259675.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGGTGAG GCCTGGGCGG CAAGCAGGGG GCAGGCCAGC CGTGCCTTTC
    CAGGCAGGAG AGGCTGCAGC CGGGGAGGAT GCCCTGTGGG GTCGGCCCAA GCGGGCAGAG
    CATTCCATGA TCTACTTCTT CCACCACTAC GAGCTGCCTG CCATCCTGCA GCAGGTCCGC
    ATCCAGGAGA TGCTGCTTCA GGCGCCGCCA CTGGGCCCCG GGACCCCCAC GGCGCTGCCC
    GATGACATGA ACAACAACTC GGGCGCCCCG GCTACAGCCC CTGACTCTGC CGGCCAGCCC
    CCCGCCCTGG GCCCCGTCTC GCCTGGGGCC AGCGGGAGTC CCGGGCCTGT GGCAGCGGCG
    CCCAGCTCCC TGGTGGCCGC GGCAGCCTCA GTGGCAGCAG CTGCCGGTGG TGACCTGGGT
    TGGATGGCAG AGACCGCTGC CATCATCACA GACGCCTCCT TCCTGTCCGG CCTGAGCGCC
    TCCCTCCTGG AGCGGCGTCC AGCCAGCCCG CTGGGCCCTG CTGGGGGCCT CCCCCACGCC
    CCCCAGGACA GTGTCCCCCC GAGTGACTCC GCAGCTTCTG ACACAACTCC CCTGGGGGCT
    GCGGTAGGCG GGCCTAGCCC GGCCTCCATG GCCCCAACGG AGGCGCCCTC GGAGGTGGGG
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51890
    Clone ID Related Accession (Same CDS sequence) XM_005259675.2 , XM_005259675.3
    Accession Version XM_005259675.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product membralin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005259675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGGTGAG GCCTGGGCGG CAAGCAGGGG GCAGGCCAGC CGTGCCTTTC
    CAGGCAGGAG AGGCTGCAGC CGGGGAGGAT GCCCTGTGGG GTCGGCCCAA GCGGGCAGAG
    CATTCCATGA TCTACTTCTT CCACCACTAC GAGCTGCCTG CCATCCTGCA GCAGGTCCGC
    ATCCAGGAGA TGCTGCTTCA GGCGCCGCCA CTGGGCCCCG GGACCCCCAC GGCGCTGCCC
    GATGACATGA ACAACAACTC GGGCGCCCCG GCTACAGCCC CTGACTCTGC CGGCCAGCCC
    CCCGCCCTGG GCCCCGTCTC GCCTGGGGCC AGCGGGAGTC CCGGGCCTGT GGCAGCGGCG
    CCCAGCTCCC TGGTGGCCGC GGCAGCCTCA GTGGCAGCAG CTGCCGGTGG TGACCTGGGT
    TGGATGGCAG AGACCGCTGC CATCATCACA GACGCCTCCT TCCTGTCCGG CCTGAGCGCC
    TCCCTCCTGG AGCGGCGTCC AGCCAGCCCG CTGGGCCCTG CTGGGGGCCT CCCCCACGCC
    CCCCAGGACA GTGTCCCCCC GAGTGACTCC GCAGCTTCTG ACACAACTCC CCTGGGGGCT
    GCGGTAGGCG GGCCTAGCCC GGCCTCCATG GCCCCAACGG AGGCGCCCTC GGAGGTGGGG
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259732.1
    CDS147..2132
    Translation

    Target ORF information:

    RefSeq Version XM_005259675.3
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259675.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGGTGAG GCCTGGGCGG CAAGCAGGGG GCAGGCCAGC CGTGCCTTTC
    CAGGCAGGAG AGGCTGCAGC CGGGGAGGAT GCCCTGTGGG GTCGGCCCAA GCGGGCAGAG
    CATTCCATGA TCTACTTCTT CCACCACTAC GAGCTGCCTG CCATCCTGCA GCAGGTCCGC
    ATCCAGGAGA TGCTGCTTCA GGCGCCGCCA CTGGGCCCCG GGACCCCCAC GGCGCTGCCC
    GATGACATGA ACAACAACTC GGGCGCCCCG GCTACAGCCC CTGACTCTGC CGGCCAGCCC
    CCCGCCCTGG GCCCCGTCTC GCCTGGGGCC AGCGGGAGTC CCGGGCCTGT GGCAGCGGCG
    CCCAGCTCCC TGGTGGCCGC GGCAGCCTCA GTGGCAGCAG CTGCCGGTGG TGACCTGGGT
    TGGATGGCAG AGACCGCTGC CATCATCACA GACGCCTCCT TCCTGTCCGG CCTGAGCGCC
    TCCCTCCTGG AGCGGCGTCC AGCCAGCCCG CTGGGCCCTG CTGGGGGCCT CCCCCACGCC
    CCCCAGGACA GTGTCCCCCC GAGTGACTCC GCAGCTTCTG ACACAACTCC CCTGGGGGCT
    GCGGTAGGCG GGCCTAGCCC GGCCTCCATG GCCCCAACGG AGGCGCCCTC GGAGGTGGGG
    TCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51891
    Clone ID Related Accession (Same CDS sequence) XM_005259676.2 , XM_005259676.3
    Accession Version XM_005259676.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product membralin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005259676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGGTG
    AGGCCTGGGC GGCAAGCAGG GGGCAGGCCA GCCGTGCCTT TCCAGGCAGG AGAGGCTGCA
    GCCGGGGAGG ATGCCCTGTG GGGTCGGCCC AAGCGGGCAG AGCATTCCAT GATCTACTTC
    TTCCACCACT ACGAGCTGCC TGCCATCCTG CAGCAGGTCC GCATCCAGGA GATGCTGCTT
    CAGGCGCCGC CACTGGGCCC CGGGACCCCC ACGGCGCTGC CCGATGACAT GAACAACAAC
    TCGGGCGCCC CGGCTACAGC CCCTGACTCT GCCGGCCAGC CCCCCGCCCT GGGCCCCGTC
    TCGCCTGGGG CCAGCGGGAG TCCCGGGCCT GTGGCAGCGG CGCCCAGCTC CCTGGTGGCC
    GCGGCAGCCT CAGTGGCAGC AGCTGCCGGT GGTGACCTGG GTTGGATGGC AGAGACCGCT
    GCCATCATCA CAGACGCCTC CTTCCTGTCC GGCCTGAGCG CCTCCCTCCT GGAGCGGCGT
    CCAGCCAGCC CGCTGGGCCC TGCTGGGGGC CTCCCCCACG CCCCCCAGGA CAGTGTCCCC
    CCGAGTGACT CCGCAGCTTC TGACACAACT CCCCTGGGGG CTGCGGTAGG CGGGCCTAGC
    CCGGCCTCCA TGGCCCCAAC GGAGGCGCCC TCGGAGGTGG GGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259733.1
    CDS174..2141
    Translation

    Target ORF information:

    RefSeq Version XM_005259676.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259676.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGGTG
    AGGCCTGGGC GGCAAGCAGG GGGCAGGCCA GCCGTGCCTT TCCAGGCAGG AGAGGCTGCA
    GCCGGGGAGG ATGCCCTGTG GGGTCGGCCC AAGCGGGCAG AGCATTCCAT GATCTACTTC
    TTCCACCACT ACGAGCTGCC TGCCATCCTG CAGCAGGTCC GCATCCAGGA GATGCTGCTT
    CAGGCGCCGC CACTGGGCCC CGGGACCCCC ACGGCGCTGC CCGATGACAT GAACAACAAC
    TCGGGCGCCC CGGCTACAGC CCCTGACTCT GCCGGCCAGC CCCCCGCCCT GGGCCCCGTC
    TCGCCTGGGG CCAGCGGGAG TCCCGGGCCT GTGGCAGCGG CGCCCAGCTC CCTGGTGGCC
    GCGGCAGCCT CAGTGGCAGC AGCTGCCGGT GGTGACCTGG GTTGGATGGC AGAGACCGCT
    GCCATCATCA CAGACGCCTC CTTCCTGTCC GGCCTGAGCG CCTCCCTCCT GGAGCGGCGT
    CCAGCCAGCC CGCTGGGCCC TGCTGGGGGC CTCCCCCACG CCCCCCAGGA CAGTGTCCCC
    CCGAGTGACT CCGCAGCTTC TGACACAACT CCCCTGGGGG CTGCGGTAGG CGGGCCTAGC
    CCGGCCTCCA TGGCCCCAAC GGAGGCGCCC TCGGAGGTGG GGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51891
    Clone ID Related Accession (Same CDS sequence) XM_005259676.2 , XM_005259676.3
    Accession Version XM_005259676.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product membralin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005259676.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGGTG
    AGGCCTGGGC GGCAAGCAGG GGGCAGGCCA GCCGTGCCTT TCCAGGCAGG AGAGGCTGCA
    GCCGGGGAGG ATGCCCTGTG GGGTCGGCCC AAGCGGGCAG AGCATTCCAT GATCTACTTC
    TTCCACCACT ACGAGCTGCC TGCCATCCTG CAGCAGGTCC GCATCCAGGA GATGCTGCTT
    CAGGCGCCGC CACTGGGCCC CGGGACCCCC ACGGCGCTGC CCGATGACAT GAACAACAAC
    TCGGGCGCCC CGGCTACAGC CCCTGACTCT GCCGGCCAGC CCCCCGCCCT GGGCCCCGTC
    TCGCCTGGGG CCAGCGGGAG TCCCGGGCCT GTGGCAGCGG CGCCCAGCTC CCTGGTGGCC
    GCGGCAGCCT CAGTGGCAGC AGCTGCCGGT GGTGACCTGG GTTGGATGGC AGAGACCGCT
    GCCATCATCA CAGACGCCTC CTTCCTGTCC GGCCTGAGCG CCTCCCTCCT GGAGCGGCGT
    CCAGCCAGCC CGCTGGGCCC TGCTGGGGGC CTCCCCCACG CCCCCCAGGA CAGTGTCCCC
    CCGAGTGACT CCGCAGCTTC TGACACAACT CCCCTGGGGG CTGCGGTAGG CGGGCCTAGC
    CCGGCCTCCA TGGCCCCAAC GGAGGCGCCC TCGGAGGTGG GGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259733.1
    CDS147..2114
    Translation

    Target ORF information:

    RefSeq Version XM_005259676.3
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259676.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGTTT GAGCTGGACA TCGAGCCCAA GGTGTTCAAG
    CCGCCGAGTA GCACAGAGGC CCTGAATGAC AGCCAGGAGT TCCCCTTCCC CGAGACGCCC
    ACCAAAGTGT GGCCGCAGGA CGAGTACATC GTGGAGTACT CACTAGAGTA TGGCTTCCTT
    CGCCTGTCGC AGGCCACCCG CCAGCGCCTG AGCATCCCCG TCATGGTGGT CACCCTGGAC
    CCCACGCGGG ACCAGTGCTT CGGGGACCGC TTCAGCCGCC TGCTGCTGGA TGAGTTCCTG
    GGCTACGATG ACATCCTCAT GTCCAGCGTG AAGGGCCTGG CCGAGAACGA GGAGAACAAG
    GGCTTCCTGC GGAATGTGGT GTCGGGCGAG CACTACCGCT TTGTGAGCAT GTGGATGGCG
    CGGACGTCCT ACCTGGCCGC CTTCGCCATC ATGGTCATCT TCACGCTGAG CGTGTCCATG
    CTGCTGCGGT ACTCACACCA CCAGATCTTC GTCTTCATCG TGGACCTGCT GCAGATGCTG
    GAGATGAACA TGGCCATCGC CTTCCCCGCA GCGCCCCTGC TGACCGTCAT CCTGGCCCTC
    GTCGGGATGG AGGCCATCAT GTCGGAGTTC TTCAACGACA CCACCACCGC CTTCTACATC
    ATCCTCATCG TGTGGCTCGC GGACCAGTAT GACGCCATCT GCTGCCACAC CAGCACCAGC
    AAGCGGCATT GGCTGCGGTT CTTCTATCTC TACCACTTCG CCTTCTATGC CTATCACTAC
    CGCTTCAATG GGCAGTATAG CAGCCTGGCC CTGGTCACCT CCTGGCTCTT CATCCAGGTG
    AGGCCTGGGC GGCAAGCAGG GGGCAGGCCA GCCGTGCCTT TCCAGGCAGG AGAGGCTGCA
    GCCGGGGAGG ATGCCCTGTG GGGTCGGCCC AAGCGGGCAG AGCATTCCAT GATCTACTTC
    TTCCACCACT ACGAGCTGCC TGCCATCCTG CAGCAGGTCC GCATCCAGGA GATGCTGCTT
    CAGGCGCCGC CACTGGGCCC CGGGACCCCC ACGGCGCTGC CCGATGACAT GAACAACAAC
    TCGGGCGCCC CGGCTACAGC CCCTGACTCT GCCGGCCAGC CCCCCGCCCT GGGCCCCGTC
    TCGCCTGGGG CCAGCGGGAG TCCCGGGCCT GTGGCAGCGG CGCCCAGCTC CCTGGTGGCC
    GCGGCAGCCT CAGTGGCAGC AGCTGCCGGT GGTGACCTGG GTTGGATGGC AGAGACCGCT
    GCCATCATCA CAGACGCCTC CTTCCTGTCC GGCCTGAGCG CCTCCCTCCT GGAGCGGCGT
    CCAGCCAGCC CGCTGGGCCC TGCTGGGGGC CTCCCCCACG CCCCCCAGGA CAGTGTCCCC
    CCGAGTGACT CCGCAGCTTC TGACACAACT CCCCTGGGGG CTGCGGTAGG CGGGCCTAGC
    CCGGCCTCCA TGGCCCCAAC GGAGGCGCCC TCGGAGGTGG GGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51892
    Clone ID Related Accession (Same CDS sequence) XM_005259677.2 , XM_005259677.3
    Accession Version XM_005259677.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product membralin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005259677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGGTGAGG CCTGGGCGGC AAGCAGGGGG CAGGCCAGCC
    GTGCCTTTCC AGGCAGGAGA GGCTGCAGCC GGGGAGGATG CCCTGTGGGG TCGGCCCAAG
    CGGGCAGAGC ATTCCATGAT CTACTTCTTC CACCACTACG AGCTGCCTGC CATCCTGCAG
    CAGGTCCGCA TCCAGGAGAT GCTGCTTCAG GCGCCGCCAC TGGGCCCCGG GACCCCCACG
    GCGCTGCCCG ATGACATGAA CAACAACTCG GGCGCCCCGG CTACAGCCCC TGACTCTGCC
    GGCCAGCCCC CCGCCCTGGG CCCCGTCTCG CCTGGGGCCA GCGGGAGTCC CGGGCCTGTG
    GCAGCGGCGC CCAGCTCCCT GGTGGCCGCG GCAGCCTCAG TGGCAGCAGC TGCCGGTGGT
    GACCTGGGTT GGATGGCAGA GACCGCTGCC ATCATCACAG ACGCCTCCTT CCTGTCCGGC
    CTGAGCGCCT CCCTCCTGGA GCGGCGTCCA GCCAGCCCGC TGGGCCCTGC TGGGGGCCTC
    CCCCACGCCC CCCAGGACAG TGTCCCCCCG AGTGACTCCG CAGCTTCTGA CACAACTCCC
    CTGGGGGCTG CGGTAGGCGG GCCTAGCCCG GCCTCCATGG CCCCAACGGA GGCGCCCTCG
    GAGGTGGGGT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259734.1
    CDS174..2108
    Translation

    Target ORF information:

    RefSeq Version XM_005259677.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259677.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGGTGAGG CCTGGGCGGC AAGCAGGGGG CAGGCCAGCC
    GTGCCTTTCC AGGCAGGAGA GGCTGCAGCC GGGGAGGATG CCCTGTGGGG TCGGCCCAAG
    CGGGCAGAGC ATTCCATGAT CTACTTCTTC CACCACTACG AGCTGCCTGC CATCCTGCAG
    CAGGTCCGCA TCCAGGAGAT GCTGCTTCAG GCGCCGCCAC TGGGCCCCGG GACCCCCACG
    GCGCTGCCCG ATGACATGAA CAACAACTCG GGCGCCCCGG CTACAGCCCC TGACTCTGCC
    GGCCAGCCCC CCGCCCTGGG CCCCGTCTCG CCTGGGGCCA GCGGGAGTCC CGGGCCTGTG
    GCAGCGGCGC CCAGCTCCCT GGTGGCCGCG GCAGCCTCAG TGGCAGCAGC TGCCGGTGGT
    GACCTGGGTT GGATGGCAGA GACCGCTGCC ATCATCACAG ACGCCTCCTT CCTGTCCGGC
    CTGAGCGCCT CCCTCCTGGA GCGGCGTCCA GCCAGCCCGC TGGGCCCTGC TGGGGGCCTC
    CCCCACGCCC CCCAGGACAG TGTCCCCCCG AGTGACTCCG CAGCTTCTGA CACAACTCCC
    CTGGGGGCTG CGGTAGGCGG GCCTAGCCCG GCCTCCATGG CCCCAACGGA GGCGCCCTCG
    GAGGTGGGGT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51892
    Clone ID Related Accession (Same CDS sequence) XM_005259677.2 , XM_005259677.3
    Accession Version XM_005259677.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product membralin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005259677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGGTGAGG CCTGGGCGGC AAGCAGGGGG CAGGCCAGCC
    GTGCCTTTCC AGGCAGGAGA GGCTGCAGCC GGGGAGGATG CCCTGTGGGG TCGGCCCAAG
    CGGGCAGAGC ATTCCATGAT CTACTTCTTC CACCACTACG AGCTGCCTGC CATCCTGCAG
    CAGGTCCGCA TCCAGGAGAT GCTGCTTCAG GCGCCGCCAC TGGGCCCCGG GACCCCCACG
    GCGCTGCCCG ATGACATGAA CAACAACTCG GGCGCCCCGG CTACAGCCCC TGACTCTGCC
    GGCCAGCCCC CCGCCCTGGG CCCCGTCTCG CCTGGGGCCA GCGGGAGTCC CGGGCCTGTG
    GCAGCGGCGC CCAGCTCCCT GGTGGCCGCG GCAGCCTCAG TGGCAGCAGC TGCCGGTGGT
    GACCTGGGTT GGATGGCAGA GACCGCTGCC ATCATCACAG ACGCCTCCTT CCTGTCCGGC
    CTGAGCGCCT CCCTCCTGGA GCGGCGTCCA GCCAGCCCGC TGGGCCCTGC TGGGGGCCTC
    CCCCACGCCC CCCAGGACAG TGTCCCCCCG AGTGACTCCG CAGCTTCTGA CACAACTCCC
    CTGGGGGCTG CGGTAGGCGG GCCTAGCCCG GCCTCCATGG CCCCAACGGA GGCGCCCTCG
    GAGGTGGGGT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259734.1
    CDS147..2081
    Translation

    Target ORF information:

    RefSeq Version XM_005259677.3
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259677.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGGTGAGG CCTGGGCGGC AAGCAGGGGG CAGGCCAGCC
    GTGCCTTTCC AGGCAGGAGA GGCTGCAGCC GGGGAGGATG CCCTGTGGGG TCGGCCCAAG
    CGGGCAGAGC ATTCCATGAT CTACTTCTTC CACCACTACG AGCTGCCTGC CATCCTGCAG
    CAGGTCCGCA TCCAGGAGAT GCTGCTTCAG GCGCCGCCAC TGGGCCCCGG GACCCCCACG
    GCGCTGCCCG ATGACATGAA CAACAACTCG GGCGCCCCGG CTACAGCCCC TGACTCTGCC
    GGCCAGCCCC CCGCCCTGGG CCCCGTCTCG CCTGGGGCCA GCGGGAGTCC CGGGCCTGTG
    GCAGCGGCGC CCAGCTCCCT GGTGGCCGCG GCAGCCTCAG TGGCAGCAGC TGCCGGTGGT
    GACCTGGGTT GGATGGCAGA GACCGCTGCC ATCATCACAG ACGCCTCCTT CCTGTCCGGC
    CTGAGCGCCT CCCTCCTGGA GCGGCGTCCA GCCAGCCCGC TGGGCCCTGC TGGGGGCCTC
    CCCCACGCCC CCCAGGACAG TGTCCCCCCG AGTGACTCCG CAGCTTCTGA CACAACTCCC
    CTGGGGGCTG CGGTAGGCGG GCCTAGCCCG GCCTCCATGG CCCCAACGGA GGCGCCCTCG
    GAGGTGGGGT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51893
    Clone ID Related Accession (Same CDS sequence) XM_005259678.2 , XM_005259678.3
    Accession Version XM_005259678.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product membralin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005259678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAGCT GCCTGCCATC
    CTGCAGCAGG TCCGCATCCA GGAGATGCTG CTTCAGGCGC CGCCACTGGG CCCCGGGACC
    CCCACGGCGC TGCCCGATGA CATGAACAAC AACTCGGGCG CCCCGGCTAC AGCCCCTGAC
    TCTGCCGGCC AGCCCCCCGC CCTGGGCCCC GTCTCGCCTG GGGCCAGCGG GAGTCCCGGG
    CCTGTGGCAG CGGCGCCCAG CTCCCTGGTG GCCGCGGCAG CCTCAGTGGC AGCAGCTGCC
    GGTGGTGACC TGGGTTGGAT GGCAGAGACC GCTGCCATCA TCACAGACGC CTCCTTCCTG
    TCCGGCCTGA GCGCCTCCCT CCTGGAGCGG CGTCCAGCCA GCCCGCTGGG CCCTGCTGGG
    GGCCTCCCCC ACGCCCCCCA GGACAGTGTC CCCCCGAGTG ACTCCGCAGC TTCTGACACA
    ACTCCCCTGG GGGCTGCGGT AGGCGGGCCT AGCCCGGCCT CCATGGCCCC AACGGAGGCG
    CCCTCGGAGG TGGGGTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259735.1
    CDS174..2054
    Translation

    Target ORF information:

    RefSeq Version XM_005259678.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259678.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAGCT GCCTGCCATC
    CTGCAGCAGG TCCGCATCCA GGAGATGCTG CTTCAGGCGC CGCCACTGGG CCCCGGGACC
    CCCACGGCGC TGCCCGATGA CATGAACAAC AACTCGGGCG CCCCGGCTAC AGCCCCTGAC
    TCTGCCGGCC AGCCCCCCGC CCTGGGCCCC GTCTCGCCTG GGGCCAGCGG GAGTCCCGGG
    CCTGTGGCAG CGGCGCCCAG CTCCCTGGTG GCCGCGGCAG CCTCAGTGGC AGCAGCTGCC
    GGTGGTGACC TGGGTTGGAT GGCAGAGACC GCTGCCATCA TCACAGACGC CTCCTTCCTG
    TCCGGCCTGA GCGCCTCCCT CCTGGAGCGG CGTCCAGCCA GCCCGCTGGG CCCTGCTGGG
    GGCCTCCCCC ACGCCCCCCA GGACAGTGTC CCCCCGAGTG ACTCCGCAGC TTCTGACACA
    ACTCCCCTGG GGGCTGCGGT AGGCGGGCCT AGCCCGGCCT CCATGGCCCC AACGGAGGCG
    CCCTCGGAGG TGGGGTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu51893
    Clone ID Related Accession (Same CDS sequence) XM_005259678.2 , XM_005259678.3
    Accession Version XM_005259678.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product membralin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005259678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAGCT GCCTGCCATC
    CTGCAGCAGG TCCGCATCCA GGAGATGCTG CTTCAGGCGC CGCCACTGGG CCCCGGGACC
    CCCACGGCGC TGCCCGATGA CATGAACAAC AACTCGGGCG CCCCGGCTAC AGCCCCTGAC
    TCTGCCGGCC AGCCCCCCGC CCTGGGCCCC GTCTCGCCTG GGGCCAGCGG GAGTCCCGGG
    CCTGTGGCAG CGGCGCCCAG CTCCCTGGTG GCCGCGGCAG CCTCAGTGGC AGCAGCTGCC
    GGTGGTGACC TGGGTTGGAT GGCAGAGACC GCTGCCATCA TCACAGACGC CTCCTTCCTG
    TCCGGCCTGA GCGCCTCCCT CCTGGAGCGG CGTCCAGCCA GCCCGCTGGG CCCTGCTGGG
    GGCCTCCCCC ACGCCCCCCA GGACAGTGTC CCCCCGAGTG ACTCCGCAGC TTCTGACACA
    ACTCCCCTGG GGGCTGCGGT AGGCGGGCCT AGCCCGGCCT CCATGGCCCC AACGGAGGCG
    CCCTCGGAGG TGGGGTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005259735.1
    CDS147..2027
    Translation

    Target ORF information:

    RefSeq Version XM_005259678.3
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005259678.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGTG
    GACCTGCTGC AGATGCTGGA GATGAACATG GCCATCGCCT TCCCCGCAGC GCCCCTGCTG
    ACCGTCATCC TGGCCCTCGT CGGGATGGAG GCCATCATGT CGGAGTTCTT CAACGACACC
    ACCACCGCCT TCTACATCAT CCTCATCGTG TGGCTCGCGG ACCAGTATGA CGCCATCTGC
    TGCCACACCA GCACCAGCAA GCGGCATTGG CTGCGGTTCT TCTATCTCTA CCACTTCGCC
    TTCTATGCCT ATCACTACCG CTTCAATGGG CAGTATAGCA GCCTGGCCCT GGTCACCTCC
    TGGCTCTTCA TCCAGCATTC CATGATCTAC TTCTTCCACC ACTACGAGCT GCCTGCCATC
    CTGCAGCAGG TCCGCATCCA GGAGATGCTG CTTCAGGCGC CGCCACTGGG CCCCGGGACC
    CCCACGGCGC TGCCCGATGA CATGAACAAC AACTCGGGCG CCCCGGCTAC AGCCCCTGAC
    TCTGCCGGCC AGCCCCCCGC CCTGGGCCCC GTCTCGCCTG GGGCCAGCGG GAGTCCCGGG
    CCTGTGGCAG CGGCGCCCAG CTCCCTGGTG GCCGCGGCAG CCTCAGTGGC AGCAGCTGCC
    GGTGGTGACC TGGGTTGGAT GGCAGAGACC GCTGCCATCA TCACAGACGC CTCCTTCCTG
    TCCGGCCTGA GCGCCTCCCT CCTGGAGCGG CGTCCAGCCA GCCCGCTGGG CCCTGCTGGG
    GGCCTCCCCC ACGCCCCCCA GGACAGTGTC CCCCCGAGTG ACTCCGCAGC TTCTGACACA
    ACTCCCCTGG GGGCTGCGGT AGGCGGGCCT AGCCCGGCCT CCATGGCCCC AACGGAGGCG
    CCCTCGGAGG TGGGGTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88807
    Clone ID Related Accession (Same CDS sequence) XM_017027457.1 , XM_017027457.2
    Accession Version XM_017027457.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product membralin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGCATTCC ATGATCTACT TCTTCCACCA CTACGAGCTG
    CCTGCCATCC TGCAGCAGGT CCGCATCCAG GAGATGCTGC TTCAGGCGCC GCCACTGGGC
    CCCGGGACCC CCACGGCGCT GCCCGATGAC ATGAACAACA ACTCGGGCGC CCCGGCTACA
    GCCCCTGACT CTGCCGGCCA GCCCCCCGCC CTGGGCCCCG TCTCGCCTGG GGCCAGCGGG
    AGTCCCGGGC CTGTGGCAGC GGCGCCCAGC TCCCTGGTGG CCGCGGCAGC CTCAGTGGCA
    GCAGCTGCCG GTGGTGACCT GGGTTGGATG GCAGAGACCG CTGCCATCAT CACAGACGCC
    TCCTTCCTGT CCGGCCTGAG CGCCTCCCTC CTGGAGCGGC GTCCAGCCAG CCCGCTGGGC
    CCTGCTGGGG GCCTCCCCCA CGCCCCCCAG GACAGTGTCC CCCCGAGTGA CTCCGCAGCT
    TCTGACACAA CTCCCCTGGG GGCTGCGGTA GGCGGGCCTA GCCCGGCCTC CATGGCCCCA
    ACGGAGGCGC CCTCGGAGGT GGGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882946.1
    CDS174..2003
    Translation

    Target ORF information:

    RefSeq Version XM_017027457.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGCATTCC ATGATCTACT TCTTCCACCA CTACGAGCTG
    CCTGCCATCC TGCAGCAGGT CCGCATCCAG GAGATGCTGC TTCAGGCGCC GCCACTGGGC
    CCCGGGACCC CCACGGCGCT GCCCGATGAC ATGAACAACA ACTCGGGCGC CCCGGCTACA
    GCCCCTGACT CTGCCGGCCA GCCCCCCGCC CTGGGCCCCG TCTCGCCTGG GGCCAGCGGG
    AGTCCCGGGC CTGTGGCAGC GGCGCCCAGC TCCCTGGTGG CCGCGGCAGC CTCAGTGGCA
    GCAGCTGCCG GTGGTGACCT GGGTTGGATG GCAGAGACCG CTGCCATCAT CACAGACGCC
    TCCTTCCTGT CCGGCCTGAG CGCCTCCCTC CTGGAGCGGC GTCCAGCCAG CCCGCTGGGC
    CCTGCTGGGG GCCTCCCCCA CGCCCCCCAG GACAGTGTCC CCCCGAGTGA CTCCGCAGCT
    TCTGACACAA CTCCCCTGGG GGCTGCGGTA GGCGGGCCTA GCCCGGCCTC CATGGCCCCA
    ACGGAGGCGC CCTCGGAGGT GGGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88807
    Clone ID Related Accession (Same CDS sequence) XM_017027457.1 , XM_017027457.2
    Accession Version XM_017027457.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product membralin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGCATTCC ATGATCTACT TCTTCCACCA CTACGAGCTG
    CCTGCCATCC TGCAGCAGGT CCGCATCCAG GAGATGCTGC TTCAGGCGCC GCCACTGGGC
    CCCGGGACCC CCACGGCGCT GCCCGATGAC ATGAACAACA ACTCGGGCGC CCCGGCTACA
    GCCCCTGACT CTGCCGGCCA GCCCCCCGCC CTGGGCCCCG TCTCGCCTGG GGCCAGCGGG
    AGTCCCGGGC CTGTGGCAGC GGCGCCCAGC TCCCTGGTGG CCGCGGCAGC CTCAGTGGCA
    GCAGCTGCCG GTGGTGACCT GGGTTGGATG GCAGAGACCG CTGCCATCAT CACAGACGCC
    TCCTTCCTGT CCGGCCTGAG CGCCTCCCTC CTGGAGCGGC GTCCAGCCAG CCCGCTGGGC
    CCTGCTGGGG GCCTCCCCCA CGCCCCCCAG GACAGTGTCC CCCCGAGTGA CTCCGCAGCT
    TCTGACACAA CTCCCCTGGG GGCTGCGGTA GGCGGGCCTA GCCCGGCCTC CATGGCCCCA
    ACGGAGGCGC CCTCGGAGGT GGGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882946.1
    CDS147..1976
    Translation

    Target ORF information:

    RefSeq Version XM_017027457.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transmembrane protein 259 (TMEM259), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTCGGAGC ACGTGGAGCC CGCAGCTCCG GGGCCCGGGC CCAACGGCGG CGGCGGCGGC 
    CCGGCCCCCG CGCGCGGGCC TCGCACCCCC AATCTCAACC CCAACCCCCT CATCAACGTG
    CGCGACCGGC TCTTCCACGC GCTGTTCTTC AAGATGGCTG TCACCTATTC GCGGCTCTTC
    CCGCCCGCCT TCCGCCGTCT CTTCGAGTTC TTCGTGCTGC TCAAGGCCCT GTTTGTGCTC
    TTCGTCCTGG CCTACATCCA CATCGTCTTC TCCCGCTCGC CCATCAACTG CCTGGAGCAT
    GTGCGTGACA AGTGGCCGCG TGAGGGCATC CTGCGTGTGG AAGTGCGGCA CAACTCGAGC
    CGCGCGCCCG TCTTCCTACA GTTCTGTGAC AGCGGCGGCC GCGGGAGCTT CCCGGGCCTG
    GCCGTGGAAC CAGGCAGCAA CCTGGACATG GAAGATGAGG AGGAGGAAGA GCTGACCATG
    GAGATGTTTG GGAACAGCTC CATCAAGGTC CCTGGTCGCC CACAGTTTGA GCTGGACATC
    GAGCCCAAGG TGTTCAAGCC GCCGAGTAGC ACAGAGGCCC TGAATGACAG CCAGGAGTTC
    CCCTTCCCCG AGACGCCCAC CAAAGTGTGG CCGCAGGACG AGTACATCGT GGAGTACTCA
    CTAGAGTATG GCTTCCTTCG CCTGTCGCAG GCCACCCGCC AGCGCCTGAG CATCCCCGTC
    ATGGTGGTCA CCCTGGACCC CACGCGGGAC CAGTGCTTCG GGGACCGCTT CAGCCGCCTG
    CTGCTGGATG AGTTCCTGGG CTACGATGAC ATCCTCATGT CCAGCGTGAA GGGCCTGGCC
    GAGAACGAGG AGAACAAGGG CTTCCTGCGG AATGTGGTGT CGGGCGAGCA CTACCGCTTT
    GTGAGCATGT GGATGGCGCG GACGTCCTAC CTGGCCGCCT TCGCCATCAT GGTCATCTTC
    ACGCTGAGCG TGTCCATGCT GCTGCGGTAC TCACACCACC AGATCTTCGT CTTCATCGCG
    CCCCTGCTGA CCGTCATCCT GGCCCTCGTC GGGATGGAGG CCATCATGTC GGAGTTCTTC
    AACGACACCA CCACCGCCTT CTACATCATC CTCATCGTGT GGCTCGCGGA CCAGTATGAC
    GCCATCTGCT GCCACACCAG CACCAGCAAG CGGCATTGGC TGCGGTTCTT CTATCTCTAC
    CACTTCGCCT TCTATGCCTA TCACTACCGC TTCAATGGGC AGTATAGCAG CCTGGCCCTG
    GTCACCTCCT GGCTCTTCAT CCAGCATTCC ATGATCTACT TCTTCCACCA CTACGAGCTG
    CCTGCCATCC TGCAGCAGGT CCGCATCCAG GAGATGCTGC TTCAGGCGCC GCCACTGGGC
    CCCGGGACCC CCACGGCGCT GCCCGATGAC ATGAACAACA ACTCGGGCGC CCCGGCTACA
    GCCCCTGACT CTGCCGGCCA GCCCCCCGCC CTGGGCCCCG TCTCGCCTGG GGCCAGCGGG
    AGTCCCGGGC CTGTGGCAGC GGCGCCCAGC TCCCTGGTGG CCGCGGCAGC CTCAGTGGCA
    GCAGCTGCCG GTGGTGACCT GGGTTGGATG GCAGAGACCG CTGCCATCAT CACAGACGCC
    TCCTTCCTGT CCGGCCTGAG CGCCTCCCTC CTGGAGCGGC GTCCAGCCAG CCCGCTGGGC
    CCTGCTGGGG GCCTCCCCCA CGCCCCCCAG GACAGTGTCC CCCCGAGTGA CTCCGCAGCT
    TCTGACACAA CTCCCCTGGG GGCTGCGGTA GGCGGGCCTA GCCCGGCCTC CATGGCCCCA
    ACGGAGGCGC CCTCGGAGGT GGGGTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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