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H2AW cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol H2AW
Entrez Gene ID 92815
Full Name histone cluster 3 H2A
General protein information
Preferred Names

histone cluster 3 H2A

Names

histone H2A type 3
histone 3, H2a
histone cluster 3, H2a
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

1

1q42.13
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015].
Disorder MIM:

615015


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_033445.2 NP_254280.1 histone H2A type 3
NM_033445.3 NP_254280.1 histone H2A type 3

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Hist1h2aa NP_068611.1
Bos taurus (cattle) LOC614881 XP_002694650.1
Homo sapiens (human) HIST3H2A NP_254280.1
Pan troglodytes (chimpanzee) HIST3H2A XP_525084.1
HIST3H2A XP_001082209.1
Mus musculus (house mouse) Hist3h2a NP_835736.1

Gene Ontology


Bibliography

Related articles in PubMed

Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligase.
Lan L, Nakajima S, Kapetanaki MG, Hsieh CL, Fagerburg M, Thickman K, Rodriguez-Collazo P, Leuba SH, Levine AS, Rapi?-Otrin V
The Journal of biological chemistry287(15)12036-49(2012 Apr)

UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.
An JY, Kim EA, Jiang Y, Zakrzewska A, Kim DE, Lee MJ, Mook-Jung I, Zhang Y, Kwon YT
Proceedings of the National Academy of Sciences of the United States of America107(5)1912-7(2010 Feb)

DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A.
Bergink S, Salomons FA, Hoogstraten D, Groothuis TA, de Waard H, Wu J, Yuan L, Citterio E, Houtsmuller AB, Neefjes J, Hoeijmakers JH, Vermeulen W, Dantuma NP
Genes & development20(10)1343-52(2006 May)

Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.
Cao R, Tsukada Y, Zhang Y
Molecular cell20(6)845-54(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following H2AW gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2AW cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu13158 NM_033445.3
Latest version!		 Homo sapiens H2A.W histone (H2AW), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
View Old Accession Versions >>
OHu13158 NM_033445.2 Homo sapiens histone cluster 3 H2A (HIST3H2A), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu13158
Clone ID Related Accession (Same CDS sequence) NM_033445.2 , NM_033445.3
Accession Version NM_033445.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 393bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-03
Organism Homo sapiens(human)
Product histone H2A type 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY131974.1. On Mar 6, 2003 this sequence version replaced NM_033445.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BM744182.1, BM849749.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGTCCGGTC GTGGTAAGCA GGGTGGCAAG GCGCGCGCCA AGGCTAAGTC GCGCTCGTCG 
CGCGCGGGGC TGCAGTTCCC CGTGGGCCGC GTGCACCGGT TGCTCCGCAA GGGCAACTAT 
TCGGAGCGCG TGGGCGCCGG CGCCCCGGTC TATCTGGCCG CGGTGCTCGA GTACTTGACT 
GCCGAGATCC TGGAGCTTGC CGGCAACGCG GCGCGCGACA ACAAGAAGAC GCGCATCATC 
CCGCGCCACC TGCAGCTGGC CATCCGCAAC GACGAGGAGC TCAACAAGCT GCTGGGCCGC 
GTGACCATCG CGCAGGGTGG CGTCCTGCCC AACATCCAGG CCGTACTGCT GCCCAAGAAG 
ACGGAGAGCC ACCACAAGGC CAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_254280.1
CDS43..435
Translation

Target ORF information:

RefSeq Version NM_033445.2
Organism Homo sapiens(human)
Definition Homo sapiens histone cluster 3 H2A (HIST3H2A), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_033445.2

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCCGGTC GTGGTAAGCA GGGTGGCAAG GCGCGCGCCA AGGCTAAGTC GCGCTCGTCG 
CGCGCGGGGC TGCAGTTCCC CGTGGGCCGC GTGCACCGGT TGCTCCGCAA GGGCAACTAT 
TCGGAGCGCG TGGGCGCCGG CGCCCCGGTC TATCTGGCCG CGGTGCTCGA GTACTTGACT 
GCCGAGATCC TGGAGCTTGC CGGCAACGCG GCGCGCGACA ACAAGAAGAC GCGCATCATC 
CCGCGCCACC TGCAGCTGGC CATCCGCAAC GACGAGGAGC TCAACAAGCT GCTGGGCCGC 
GTGACCATCG CGCAGGGTGG CGTCCTGCCC AACATCCAGG CCGTACTGCT GCCCAAGAAG 
ACGGAGAGCC ACCACAAGGC CAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13158
Clone ID Related Accession (Same CDS sequence) NM_033445.2 , NM_033445.3
Accession Version NM_033445.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 393bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2020-01-04
Organism Homo sapiens(human)
Product histone H2A type 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL139288.15. On Dec 3, 2019 this sequence version replaced NM_033445.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BM744182.1, DA092328.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript replication-dependent histone :: PMID: 12408966 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGTCCGGTC GTGGTAAGCA GGGTGGCAAG GCGCGCGCCA AGGCTAAGTC GCGCTCGTCG 
CGCGCGGGGC TGCAGTTCCC CGTGGGCCGC GTGCACCGGT TGCTCCGCAA GGGCAACTAT 
TCGGAGCGCG TGGGCGCCGG CGCCCCGGTC TATCTGGCCG CGGTGCTCGA GTACTTGACT 
GCCGAGATCC TGGAGCTTGC CGGCAACGCG GCGCGCGACA ACAAGAAGAC GCGCATCATC 
CCGCGCCACC TGCAGCTGGC CATCCGCAAC GACGAGGAGC TCAACAAGCT GCTGGGCCGC 
GTGACCATCG CGCAGGGTGG CGTCCTGCCC AACATCCAGG CCGTACTGCT GCCCAAGAAG 
ACGGAGAGCC ACCACAAGGC CAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_254280.1
CDS57..449
Translation

Target ORF information:

RefSeq Version NM_033445.3
Organism Homo sapiens(human)
Definition Homo sapiens H2A.W histone (H2AW), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_033445.3

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCCGGTC GTGGTAAGCA GGGTGGCAAG GCGCGCGCCA AGGCTAAGTC GCGCTCGTCG 
CGCGCGGGGC TGCAGTTCCC CGTGGGCCGC GTGCACCGGT TGCTCCGCAA GGGCAACTAT 
TCGGAGCGCG TGGGCGCCGG CGCCCCGGTC TATCTGGCCG CGGTGCTCGA GTACTTGACT 
GCCGAGATCC TGGAGCTTGC CGGCAACGCG GCGCGCGACA ACAAGAAGAC GCGCATCATC 
CCGCGCCACC TGCAGCTGGC CATCCGCAAC GACGAGGAGC TCAACAAGCT GCTGGGCCGC 
GTGACCATCG CGCAGGGTGG CGTCCTGCCC AACATCCAGG CCGTACTGCT GCCCAAGAAG 
ACGGAGAGCC ACCACAAGGC CAAGGGCAAG TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Monoubiquitinated histone H2A destabilizes photolesion-containing nucleosomes with concomitant release of UV-damaged DNA-binding protein E3 ligase.
The Journal of biological chemistry287(15)12036-49(2012 Apr)
Lan L,Nakajima S,Kapetanaki MG,Hsieh CL,Fagerburg M,Thickman K,Rodriguez-Collazo P,Leuba SH,Levine AS,Rapi?-Otrin V


book

UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination.
Proceedings of the National Academy of Sciences of the United States of America107(5)1912-7(2010 Feb)
An JY,Kim EA,Jiang Y,Zakrzewska A,Kim DE,Lee MJ,Mook-Jung I,Zhang Y,Kwon YT


book

DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A.
Genes & development20(10)1343-52(2006 May)
Bergink S,Salomons FA,Hoogstraten D,Groothuis TA,de Waard H,Wu J,Yuan L,Citterio E,Houtsmuller AB,Neefjes J,Hoeijmakers JH,Vermeulen W,Dantuma NP


book

Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.
Molecular cell20(6)845-54(2005 Dec)
Cao R,Tsukada Y,Zhang Y