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VAMP3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol VAMP3
Entrez Gene ID 9341
Full Name vesicle associated membrane protein 3
Synonyms CEB
General protein information
Preferred Names

vesicle associated membrane protein 3

Names

vesicle-associated membrane protein 3
VAMP-3
cellubrevin
synaptobrevin-3

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

1

1p36.23

Summary Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008].
Disorder MIM:

603657


mRNA and Protein(s)

mRNA Protein Name
NM_004781.3 NP_004772.1 vesicle-associated membrane protein 3
XM_017002841.1 XP_016858330.1 vesicle-associated membrane protein 3 isoform X1



Macaca mulatta (rhesus monkey) VAMP3 XP_001095950.1
Danio rerio (zebrafish) vamp3 NP_001002073.1
Gallus gallus (chicken) VAMP3 NP_001034578.1
Homo sapiens (human) VAMP3 NP_004772.1
Canis lupus familiaris (dog) VAMP3 XP_005620536.1
Mus musculus (house mouse) Vamp3 NP_033524.1
Bos taurus (cattle) VAMP3 NP_001039380.1
Pan troglodytes (chimpanzee) VAMP3 NP_001233512.1
Rattus norvegicus (Norway rat) Vamp3 NP_476438.1
Xenopus tropicalis (tropical clawed frog) vamp3 NP_001016972.1


Related articles in PubMed

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs).
Kuster A, Nola S, Dingli F, Vacca B, Gauchy C, Beaujouan JC, Nunez M, Moncion T, Loew D, Formstecher E, Galli T, Proux-Gillardeaux V
The Journal of biological chemistry290(47)28056-69(2015 Nov)

The small GTPase Rab8 interacts with VAMP-3 to regulate the delivery of recycling T-cell receptors to the immune synapse.
Finetti F, Patrussi L, Galgano D, Cassioli C, Perinetti G, Pazour GJ, Baldari CT
Journal of cell science128(14)2541-52(2015 Jul)

Human herpesvirus 6 gM/gN complex interacts with v-SNARE in infected cells.
Kawabata A, Serada S, Naka T, Mori Y
The Journal of general virology95(Pt 12)2769-77(2014 Dec)

Role of VAMP3 and VAMP7 in the commitment of Yersinia pseudotuberculosis to LC3-associated pathways involving single- or double-membrane vacuoles.
Ligeon LA, Moreau K, Barois N, Bongiovanni A, Lacorre DA, Werkmeister E, Proux-Gillardeaux V, Galli T, Lafont F
Autophagy10(9)1588-602(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The small GTPase Rab8 interacts with VAMP-3 to regulate the delivery of recycling T-cell receptors to the immune synapse.
Title: The small GTPase Rab8 interacts with VAMP-3 to regulate the delivery of recycling T-cell receptors to the immune synapse.

Liquid chromatography-MS/MS analysis showed that the HHV-6 gM/gN complex interacts with the v-SNARE protein, vesicle-associated membrane protein 3 (VAMP3).
Title: Human herpesvirus 6 gM/gN complex interacts with v-SNARE in infected cells.

highlight the role that VAMP3 and VAMP7 play in selection of the pathways leading to generation of ultrastructurally different LC3 compartments
Title: Role of VAMP3 and VAMP7 in the commitment of Yersinia pseudotuberculosis to LC3-associated pathways involving single- or double-membrane vacuoles.

phosphatidylinositol 4-kinase IIalpha knockdown inhibited vesicle-associated membrane protein 3 trafficking to perinuclear membranes and impaired the rate of VAMP3-mediated recycling of the transferrin receptor
Title: Endosomal sorting of VAMP3 is regulated by PI4K2A.

These results indicated that Uukuniemi virus relies on VAMP3 for penetration, providing an indication of added complexity in the trafficking of viruses through the endocytic network.
Title: Genome-wide small interfering RNA screens reveal VAMP3 as a novel host factor required for Uukuniemi virus late penetration.

The following VAMP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAMP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu15733 NM_004781.3
Latest version!
Homo sapiens vesicle associated membrane protein 3 (VAMP3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu90807 XM_017002841.1
Latest version!
Homo sapiens vesicle associated membrane protein 3 (VAMP3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15733
Clone ID Related Accession (Same CDS sequence) NM_004781.3
Accession Version NM_004781.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 303bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-26
Organism Homo sapiens(Human)
Product vesicle-associated membrane protein 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005941.1 and BC003570.1. On Feb 13, 2004 this sequence version replaced NM_004781.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.98320.1, SRR1803617.274687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGTCTACAG GTCCAACTGC TGCCACTGGC AGTAATCGAA GACTTCAGCA GACACAAAAT 
CAAGTAGATG AGGTGGTGGA CATAATGCGA GTTAACGTGG ACAAGGTTCT GGAAAGAGAC
CAGAAGCTCT CTGAGTTAGA CGACCGTGCA GACGCACTGC AGGCAGGCGC TTCTCAATTT
GAAACGAGCG CAGCCAAGTT GAAGAGGAAA TATTGGTGGA AGAATTGCAA GATGTGGGCA
ATCGGGATTA CTGTTCTGGT TATCTTCATC ATCATCATCA TCGTGTGGGT TGTCTCTTCA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004772.1
CDS116..418
Misc Feature(1)74..76
Misc Feature(2)119..121
Misc Feature(3)146..>358
Misc Feature(4)152..340
Misc Feature(5)order(155..163,167..172,176..193,197..205,209..211,218..223,227..235,239..256,260..277,281..298,302..319,323..331,338..340)
Misc Feature(6)230..232
Misc Feature(7)347..409
Exon (1)1..117
Exon (2)118..187
Exon (3)188..346
Exon (4)347..398
Exon (5)399..2205
Translation

Target ORF information:

RefSeq Version NM_004781.3
Organism Homo sapiens(Human)
Definition Homo sapiens vesicle associated membrane protein 3 (VAMP3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004781.3

ORF Insert Sequence:

1
61
121
181
241
301
ATGTCTACAG GTCCAACTGC TGCCACTGGC AGTAATCGAA GACTTCAGCA GACACAAAAT 
CAAGTAGATG AGGTGGTGGA CATAATGCGA GTTAACGTGG ACAAGGTTCT GGAAAGAGAC
CAGAAGCTCT CTGAGTTAGA CGACCGTGCA GACGCACTGC AGGCAGGCGC TTCTCAATTT
GAAACGAGCG CAGCCAAGTT GAAGAGGAAA TATTGGTGGA AGAATTGCAA GATGTGGGCA
ATCGGGATTA CTGTTCTGGT TATCTTCATC ATCATCATCA TCGTGTGGGT TGTCTCTTCA
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu90807
Clone ID Related Accession (Same CDS sequence) XM_017002841.1
Accession Version XM_017002841.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 369bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product vesicle-associated membrane protein 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
ATGTCTACAG GTCCAACTGC TGCCACTGGC AGTAATCGAA GACTTCAGCA GACACAAAAT 
CAAGTAGATG AGGTGGTGGA CATAATGCGA GTTAACGTGG ACAAGGTTCT GGAAAGAGAC
CAGAAGCTCT CTGAGTTAGA CGACCGTGCA GACGCACTGC AGGCAGGCGC TTCTCAATTT
GAAACGAGCG CAGCCAAGTT GAAGAGGAAA TATTGGTGGA AGAATTGCAA GATGTGGGCA
ATCGGGATTA CTGTTCTGGT TATCTTCATC ATCATCATCA TCGGTGAGTT ACCCTTTTCT
AAACTGATTG GAAAAGTCTT CTCCATGTGT TCACAGACCA TTGATTTTGT GTACAAGAAC
TCAGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016858330.1
CDS110..478
Translation

Target ORF information:

RefSeq Version XM_017002841.1
Organism Homo sapiens(Human)
Definition Homo sapiens vesicle associated membrane protein 3 (VAMP3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017002841.1

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCTACAG GTCCAACTGC TGCCACTGGC AGTAATCGAA GACTTCAGCA GACACAAAAT 
CAAGTAGATG AGGTGGTGGA CATAATGCGA GTTAACGTGG ACAAGGTTCT GGAAAGAGAC
CAGAAGCTCT CTGAGTTAGA CGACCGTGCA GACGCACTGC AGGCAGGCGC TTCTCAATTT
GAAACGAGCG CAGCCAAGTT GAAGAGGAAA TATTGGTGGA AGAATTGCAA GATGTGGGCA
ATCGGGATTA CTGTTCTGGT TATCTTCATC ATCATCATCA TCGGTGAGTT ACCCTTTTCT
AAACTGATTG GAAAAGTCTT CTCCATGTGT TCACAGACCA TTGATTTTGT GTACAAGAAC
TCAGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP


book

The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs).
The Journal of biological chemistry290(47)28056-69(2015 Nov)
Kuster A,Nola S,Dingli F,Vacca B,Gauchy C,Beaujouan JC,Nunez M,Moncion T,Loew D,Formstecher E,Galli T,Proux-Gillardeaux V


book

The small GTPase Rab8 interacts with VAMP-3 to regulate the delivery of recycling T-cell receptors to the immune synapse.
Journal of cell science128(14)2541-52(2015 Jul)
Finetti F,Patrussi L,Galgano D,Cassioli C,Perinetti G,Pazour GJ,Baldari CT


book

Human herpesvirus 6 gM/gN complex interacts with v-SNARE in infected cells.
The Journal of general virology95(Pt 12)2769-77(2014 Dec)
Kawabata A,Serada S,Naka T,Mori Y


book

Role of VAMP3 and VAMP7 in the commitment of Yersinia pseudotuberculosis to LC3-associated pathways involving single- or double-membrane vacuoles.
Autophagy10(9)1588-602(2014 Sep)
Ligeon LA,Moreau K,Barois N,Bongiovanni A,Lacorre DA,Werkmeister E,Proux-Gillardeaux V,Galli T,Lafont F


 
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