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KL cDNA ORF clone, Homo sapiens(Human)

Gene Symbol KL
Entrez Gene ID 9365
Full Name klotho
General protein information
Preferred Names

klotho

Names

klotho

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

13

13q13.1

Summary This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008].
Disorder MIM:

604824

Disorder Html: {Coronary artery disease, susceptibility to} (3); Tumoral calcinosis,

mRNA and Protein(s)

mRNA Protein Name
XM_006719895.2 XP_006719958.1 klotho isoform X1
NM_004795.3 NP_004786.2 klotho precursor



Pan troglodytes (chimpanzee) KL XP_522655.2
Macaca mulatta (rhesus monkey) LOC714042 XP_001101127.1
Homo sapiens (human) KL NP_004786.2
Canis lupus familiaris (dog) KL XP_003433357.1
Bos taurus (cattle) KL NP_001178124.1
Mus musculus (house mouse) Kl NP_038851.2
Danio rerio (zebrafish) kl XP_690797.4
Rattus norvegicus (Norway rat) Kl NP_112626.1
Gallus gallus (chicken) KL XP_004938814.1
Xenopus tropicalis (tropical clawed frog) kl XP_002934067.1


Related articles in PubMed

Association Study of Klotho Gene Polymorphism With Calcium Oxalate Stones in The Uyghur Population of Xinjiang, China.
Ali A, Tursun H, Talat A, Abla A, Muhtar E, Zhang T, Mahmut M
Urology journal14(1)2939-2943(2017 Jan)

Decreased Level of Klotho Contributes to Drug Resistance in Lung Cancer Cells: Involving in Klotho-Mediated Cell Autophagy.
Chen T, Ren H, Thakur A, Yang T, Li Y, Zhang S, Wang T, Chen M
DNA and cell biology35(12)751-757(2016 Dec)

Clinical significance of α‑ and β‑Klotho in urothelial carcinoma of the bladder.
Hori S, Miyake M, Onishi S, Tatsumi Y, Morizawa Y, Nakai Y, Anai S, Tanaka N, Fujimoto K
Oncology reports36(4)2117-25(2016 Oct)

Soluble α-Klotho - a new marker of acromegaly?
Jawiarczyk-Przybyłowska A, Halupczok-Żyła J, Bolanowski M
Endokrynologia Polska67(4)390-6(2016)

Changes in Serum Concentrations of Fibroblast Growth Factor 23 and Soluble Klotho in Hemodialysis Patients after Total Parathyroidectomy.
Liao SC, Moi SH, Chou FF, Yang CH, Chen JB
BioMed research international20166453803(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Klotho levels were higher in acute kidney injury patients and females and lower in end stage renal disease patients than in healthy adults and patients with moderate chronic kidney disease.
Title: Serum klotho levels in acute kidney injury
.

Data suggest Klotho attenuates ox-LDL- (oxidized low density lipoprotein)-induced oxidative stress in vascular endothelial cells via up-regulation of oxidative scavengers (lipoprotein and nitric oxide), activation of the PI3K/Akt/eNOS signaling, and down-regulation of LOX1 (lectin-like ox-LDL receptor) expression. (PI3K = phosphatidylinositol 3-Kinase; Akt = proto-oncogene c-Akt; eNOS = endothelial nitric oxide synthase)
Title: Klotho ameliorates oxidized low density lipoprotein (ox-LDL)-induced oxidative stress via regulating LOX-1 and PI3K/Akt/eNOS pathways.

disruption of the FGF23-Klotho system causes phosphate retention and a syndrome resembling aging in mammals has led to the notion that phosphate accelerates aging--{REVIEW}
Title: The FGF23 and Klotho system beyond mineral metabolism.

Results showed that human endothelial cells differentially express functional full-length alpha-Klotho protein. And that it is this full-length alpha-Klotho protein that modulates endothelial cells response to FGF-23.
Title: α-Klotho expression determines nitric oxide synthesis in response to FGF-23 in human aortic endothelial cells.

There was no change in circulating FGF23 and Klotho concentrations after PTX in hemodialysis patients given postoperative calcium supplements and/or vitamin D analogue. Serum FGF23 concentrations pre-PTX and at days 5 and 90 after PTX were inversely related to serum calcium concentrations.
Title: Changes in Serum Concentrations of Fibroblast Growth Factor 23 and Soluble Klotho in Hemodialysis Patients after Total Parathyroidectomy.

The following KL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18278 NM_004795.3
Latest version!
Homo sapiens klotho (KL), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$399.50-$559.30
$799.00
OHu47313 XM_006719895.2
Latest version!
Homo sapiens klotho (KL), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18278
Clone ID Related Accession (Same CDS sequence) NM_004795.3
Accession Version NM_004795.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3039bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-04
Organism Homo sapiens(Human)
Product klotho precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161898.17, AB005142.1 and AA890465.1. This sequence is a reference standard in the RefSeqGene project. On Oct 16, 2008 this sequence version replaced NM_004795.2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB005142.1, SRR1803611.233700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
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121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGCCCGCCA GCGCCCCGCC GCGCCGCCCG CGGCCGCCGC CGCCGTCGCT GTCGCTGCTG 
CTGGTGCTGC TGGGCCTGGG CGGCCGCCGC CTGCGTGCGG AGCCGGGCGA CGGCGCGCAG
ACCTGGGCCC GTTTCTCGCG GCCTCCTGCC CCCGAGGCCG CGGGCCTCTT CCAGGGCACC
TTCCCCGACG GCTTCCTCTG GGCCGTGGGC AGCGCCGCCT ACCAGACCGA GGGCGGCTGG
CAGCAGCACG GCAAGGGTGC GTCCATCTGG GATACGTTCA CCCACCACCC CCTGGCACCC
CCGGGAGACT CCCGGAACGC CAGTCTGCCG TTGGGCGCCC CGTCGCCGCT GCAGCCCGCC
ACCGGGGACG TAGCCAGCGA CAGCTACAAC AACGTCTTCC GCGACACGGA GGCGCTGCGC
GAGCTCGGGG TCACTCACTA CCGCTTCTCC ATCTCGTGGG CGCGAGTGCT CCCCAATGGC
AGCGCGGGCG TCCCCAACCG CGAGGGGCTG CGCTACTACC GGCGCCTGCT GGAGCGGCTG
CGGGAGCTGG GCGTGCAGCC CGTGGTCACC CTGTACCACT GGGACCTGCC CCAGCGCCTG
CAGGACGCCT ACGGCGGCTG GGCCAACCGC GCCCTGGCCG ACCACTTCAG GGATTACGCG
GAGCTCTGCT TCCGCCACTT CGGCGGTCAG GTCAAGTACT GGATCACCAT CGACAACCCC
TACGTGGTGG CCTGGCACGG CTACGCCACC GGGCGCCTGG CCCCCGGCAT CCGGGGCAGC
CCGCGGCTCG GGTACCTGGT GGCGCACAAC CTCCTCCTGG CTCATGCCAA AGTCTGGCAT
CTCTACAATA CTTCTTTCCG TCCCACTCAG GGAGGTCAGG TGTCCATTGC CCTAAGCTCT
CACTGGATCA ATCCTCGAAG AATGACCGAC CACAGCATCA AAGAATGTCA AAAATCTCTG
GACTTTGTAC TAGGTTGGTT TGCCAAACCC GTATTTATTG ATGGTGACTA TCCCGAGAGC
ATGAAGAATA ACCTTTCATC TATTCTGCCT GATTTTACTG AATCTGAGAA AAAGTTCATC
AAAGGAACTG CTGACTTTTT TGCTCTTTGC TTTGGACCCA CCTTGAGTTT TCAACTTTTG
GACCCTCACA TGAAGTTCCG CCAATTGGAA TCTCCCAACC TGAGGCAACT GCTTTCCTGG
ATTGACCTTG AATTTAACCA TCCTCAAATA TTTATTGTGG AAAATGGCTG GTTTGTCTCA
GGGACCACCA AGAGAGATGA TGCCAAATAT ATGTATTACC TCAAAAAGTT CATCATGGAA
ACCTTAAAAG CCATCAAGCT GGATGGGGTG GATGTCATCG GGTATACCGC ATGGTCCCTC
ATGGATGGTT TCGAGTGGCA CAGAGGTTAC AGCATCAGGC GTGGACTCTT CTATGTTGAC
TTTCTAAGCC AGGACAAGAT GTTGTTGCCA AAGTCTTCAG CCTTGTTCTA CCAAAAGCTG
ATAGAGAAAA ATGGCTTCCC TCCTTTACCT GAAAATCAGC CCCTAGAAGG GACATTTCCC
TGTGACTTTG CTTGGGGAGT TGTTGACAAC TACATTCAAG TAGATACCAC TCTGTCTCAG
TTTACCGACC TGAATGTTTA CCTGTGGGAT GTCCACCACA GTAAAAGGCT TATTAAAGTG
GATGGGGTTG TGACCAAGAA GAGGAAATCC TACTGTGTTG ACTTTGCTGC CATCCAGCCC
CAGATCGCTT TACTCCAGGA AATGCACGTT ACACATTTTC GCTTCTCCCT GGACTGGGCC
CTGATTCTCC CTCTGGGTAA CCAGTCCCAG GTGAACCACA CCATCCTGCA GTACTATCGC
TGCATGGCCA GCGAGCTTGT CCGTGTCAAC ATCACCCCAG TGGTGGCCCT GTGGCAGCCT
ATGGCCCCGA ACCAAGGACT GCCGCGCCTC CTGGCCAGGC AGGGCGCCTG GGAGAACCCC
TACACTGCCC TGGCCTTTGC AGAGTATGCC CGACTGTGCT TTCAAGAGCT CGGCCATCAC
GTCAAGCTTT GGATAACGAT GAATGAGCCG TATACAAGGA ATATGACATA CAGTGCTGGC
CACAACCTTC TGAAGGCCCA TGCCCTGGCT TGGCATGTGT ACAATGAAAA GTTTAGGCAT
GCTCAGAATG GGAAAATATC CATAGCCTTG CAGGCTGATT GGATAGAACC TGCCTGCCCT
TTCTCCCAAA AGGACAAAGA GGTGGCTGAG AGAGTTTTGG AATTTGACAT TGGCTGGCTG
GCTGAGCCCA TTTTCGGCTC TGGAGATTAT CCATGGGTGA TGAGGGACTG GCTGAACCAA
AGAAACAATT TTCTTCTTCC TTATTTCACT GAAGATGAAA AAAAGCTAAT CCAGGGTACC
TTTGACTTTT TGGCTTTAAG CCATTATACC ACCATCCTTG TAGACTCAGA AAAAGAAGAT
CCAATAAAAT ACAATGATTA CCTAGAAGTG CAAGAAATGA CCGACATCAC GTGGCTCAAC
TCCCCCAGTC AGGTGGCGGT AGTGCCCTGG GGGTTGCGCA AAGTGCTGAA CTGGCTGAAG
TTCAAGTACG GAGACCTCCC CATGTACATA ATATCCAATG GAATCGATGA CGGGCTGCAT
GCTGAGGACG ACCAGCTGAG GGTGTATTAT ATGCAGAATT ACATAAACGA AGCTCTCAAA
GCCCACATAC TGGATGGTAT CAATCTTTGC GGATACTTTG CTTATTCGTT TAACGACCGC
ACAGCTCCGA GGTTTGGCCT CTATCGTTAT GCTGCAGATC AGTTTGAGCC CAAGGCATCC
ATGAAACATT ACAGGAAAAT TATTGACAGC AATGGTTTCC CGGGCCCAGA AACTCTGGAA
AGATTTTGTC CAGAAGAATT CACCGTGTGT ACTGAGTGCA GTTTTTTTCA CACCCGAAAG
TCTTTACTGG CTTTCATAGC TTTTCTATTT TTTGCTTCTA TTATTTCTCT CTCCCTTATA
TTTTACTACT CGAAGAAAGG CAGAAGAAGT TACAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004786.2
CDS9..3047
Misc Feature(1)177..1526
Misc Feature(2)180..1526
Misc Feature(3)1455..1457
Misc Feature(4)1551..2867
Misc Feature(5)1557..2867
Misc Feature(6)2952..3014
Exon (1)1..827
Exon (2)828..1338
Exon (3)1339..1607
Exon (4)1608..2709
Exon (5)2710..5006
Translation

Target ORF information:

RefSeq Version NM_004795.3
Organism Homo sapiens(Human)
Definition Homo sapiens klotho (KL), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004795.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
ATGCCCGCCA GCGCCCCGCC GCGCCGCCCG CGGCCGCCGC CGCCGTCGCT GTCGCTGCTG 
CTGGTGCTGC TGGGCCTGGG CGGCCGCCGC CTGCGTGCGG AGCCGGGCGA CGGCGCGCAG
ACCTGGGCCC GTTTCTCGCG GCCTCCTGCC CCCGAGGCCG CGGGCCTCTT CCAGGGCACC
TTCCCCGACG GCTTCCTCTG GGCCGTGGGC AGCGCCGCCT ACCAGACCGA GGGCGGCTGG
CAGCAGCACG GCAAGGGTGC GTCCATCTGG GATACGTTCA CCCACCACCC CCTGGCACCC
CCGGGAGACT CCCGGAACGC CAGTCTGCCG TTGGGCGCCC CGTCGCCGCT GCAGCCCGCC
ACCGGGGACG TAGCCAGCGA CAGCTACAAC AACGTCTTCC GCGACACGGA GGCGCTGCGC
GAGCTCGGGG TCACTCACTA CCGCTTCTCC ATCTCGTGGG CGCGAGTGCT CCCCAATGGC
AGCGCGGGCG TCCCCAACCG CGAGGGGCTG CGCTACTACC GGCGCCTGCT GGAGCGGCTG
CGGGAGCTGG GCGTGCAGCC CGTGGTCACC CTGTACCACT GGGACCTGCC CCAGCGCCTG
CAGGACGCCT ACGGCGGCTG GGCCAACCGC GCCCTGGCCG ACCACTTCAG GGATTACGCG
GAGCTCTGCT TCCGCCACTT CGGCGGTCAG GTCAAGTACT GGATCACCAT CGACAACCCC
TACGTGGTGG CCTGGCACGG CTACGCCACC GGGCGCCTGG CCCCCGGCAT CCGGGGCAGC
CCGCGGCTCG GGTACCTGGT GGCGCACAAC CTCCTCCTGG CTCATGCCAA AGTCTGGCAT
CTCTACAATA CTTCTTTCCG TCCCACTCAG GGAGGTCAGG TGTCCATTGC CCTAAGCTCT
CACTGGATCA ATCCTCGAAG AATGACCGAC CACAGCATCA AAGAATGTCA AAAATCTCTG
GACTTTGTAC TAGGTTGGTT TGCCAAACCC GTATTTATTG ATGGTGACTA TCCCGAGAGC
ATGAAGAATA ACCTTTCATC TATTCTGCCT GATTTTACTG AATCTGAGAA AAAGTTCATC
AAAGGAACTG CTGACTTTTT TGCTCTTTGC TTTGGACCCA CCTTGAGTTT TCAACTTTTG
GACCCTCACA TGAAGTTCCG CCAATTGGAA TCTCCCAACC TGAGGCAACT GCTTTCCTGG
ATTGACCTTG AATTTAACCA TCCTCAAATA TTTATTGTGG AAAATGGCTG GTTTGTCTCA
GGGACCACCA AGAGAGATGA TGCCAAATAT ATGTATTACC TCAAAAAGTT CATCATGGAA
ACCTTAAAAG CCATCAAGCT GGATGGGGTG GATGTCATCG GGTATACCGC ATGGTCCCTC
ATGGATGGTT TCGAGTGGCA CAGAGGTTAC AGCATCAGGC GTGGACTCTT CTATGTTGAC
TTTCTAAGCC AGGACAAGAT GTTGTTGCCA AAGTCTTCAG CCTTGTTCTA CCAAAAGCTG
ATAGAGAAAA ATGGCTTCCC TCCTTTACCT GAAAATCAGC CCCTAGAAGG GACATTTCCC
TGTGACTTTG CTTGGGGAGT TGTTGACAAC TACATTCAAG TAGATACCAC TCTGTCTCAG
TTTACCGACC TGAATGTTTA CCTGTGGGAT GTCCACCACA GTAAAAGGCT TATTAAAGTG
GATGGGGTTG TGACCAAGAA GAGGAAATCC TACTGTGTTG ACTTTGCTGC CATCCAGCCC
CAGATCGCTT TACTCCAGGA AATGCACGTT ACACATTTTC GCTTCTCCCT GGACTGGGCC
CTGATTCTCC CTCTGGGTAA CCAGTCCCAG GTGAACCACA CCATCCTGCA GTACTATCGC
TGCATGGCCA GCGAGCTTGT CCGTGTCAAC ATCACCCCAG TGGTGGCCCT GTGGCAGCCT
ATGGCCCCGA ACCAAGGACT GCCGCGCCTC CTGGCCAGGC AGGGCGCCTG GGAGAACCCC
TACACTGCCC TGGCCTTTGC AGAGTATGCC CGACTGTGCT TTCAAGAGCT CGGCCATCAC
GTCAAGCTTT GGATAACGAT GAATGAGCCG TATACAAGGA ATATGACATA CAGTGCTGGC
CACAACCTTC TGAAGGCCCA TGCCCTGGCT TGGCATGTGT ACAATGAAAA GTTTAGGCAT
GCTCAGAATG GGAAAATATC CATAGCCTTG CAGGCTGATT GGATAGAACC TGCCTGCCCT
TTCTCCCAAA AGGACAAAGA GGTGGCTGAG AGAGTTTTGG AATTTGACAT TGGCTGGCTG
GCTGAGCCCA TTTTCGGCTC TGGAGATTAT CCATGGGTGA TGAGGGACTG GCTGAACCAA
AGAAACAATT TTCTTCTTCC TTATTTCACT GAAGATGAAA AAAAGCTAAT CCAGGGTACC
TTTGACTTTT TGGCTTTAAG CCATTATACC ACCATCCTTG TAGACTCAGA AAAAGAAGAT
CCAATAAAAT ACAATGATTA CCTAGAAGTG CAAGAAATGA CCGACATCAC GTGGCTCAAC
TCCCCCAGTC AGGTGGCGGT AGTGCCCTGG GGGTTGCGCA AAGTGCTGAA CTGGCTGAAG
TTCAAGTACG GAGACCTCCC CATGTACATA ATATCCAATG GAATCGATGA CGGGCTGCAT
GCTGAGGACG ACCAGCTGAG GGTGTATTAT ATGCAGAATT ACATAAACGA AGCTCTCAAA
GCCCACATAC TGGATGGTAT CAATCTTTGC GGATACTTTG CTTATTCGTT TAACGACCGC
ACAGCTCCGA GGTTTGGCCT CTATCGTTAT GCTGCAGATC AGTTTGAGCC CAAGGCATCC
ATGAAACATT ACAGGAAAAT TATTGACAGC AATGGTTTCC CGGGCCCAGA AACTCTGGAA
AGATTTTGTC CAGAAGAATT CACCGTGTGT ACTGAGTGCA GTTTTTTTCA CACCCGAAAG
TCTTTACTGG CTTTCATAGC TTTTCTATTT TTTGCTTCTA TTATTTCTCT CTCCCTTATA
TTTTACTACT CGAAGAAAGG CAGAAGAAGT TACAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47313
Clone ID Related Accession (Same CDS sequence) XM_006719895.2
Accession Version XM_006719895.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product klotho isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGACC ACAGCATCAA AGAATGTCAA AAATCTCTGG ACTTTGTACT AGGTTGGTTT 
GCCAAACCCG TATTTATTGA TGGTGACTAT CCCGAGAGCA TGAAGAATAA CCTTTCATCT
ATTCTGCCTG ATTTTACTGA ATCTGAGAAA AAGTTCATCA AAGGAACTGC TGACTTTTTT
GCTCTTTGCT TTGGACCCAC CTTGAGTTTT CAACTTTTGG ACCCTCACAT GAAGTTCCGC
CAATTGGAAT CTCCCAACCT GAGGCAACTG CTTTCCTGGA TTGACCTTGA ATTTAACCAT
CCTCAAATAT TTATTGTGGA AAATGGCTGG TTTGTCTCAG GGACCACCAA GAGAGATGAT
GCCAAATATA TGTATTACCT CAAAAAGTTC ATCATGGAAA CCTTAAAAGC CATCAAGCTG
GATGGGGTGG ATGTCATCGG GTATACCGCA TGGTCCCTCA TGGATGGTTT CGAGTGGCAC
AGAGGTTACA GCATCAGGCG TGGACTCTTC TATGTTGACT TTCTAAGCCA GGACAAGATG
TTGTTGCCAA AGTCTTCAGC CTTGTTCTAC CAAAAGCTGA TAGAGAAAAA TGGCTTCCCT
CCTTTACCTG AAAATCAGCC CCTAGAAGGG ACATTTCCCT GTGACTTTGC TTGGGGAGTT
GTTGACAACT ACATTCAAGT AGATACCACT CTGTCTCAGT TTACCGACCT GAATGTTTAC
CTGTGGGATG TCCACCACAG TAAAAGGCTT ATTAAAGTGG ATGGGGTTGT GACCAAGAAG
AGGAAATCCT ACTGTGTTGA CTTTGCTGCC ATCCAGCCCC AGATCGCTTT ACTCCAGGAA
ATGCACGTTA CACATTTTCG CTTCTCCCTG GACTGGGCCC TGATTCTCCC TCTGGGTAAC
CAGTCCCAGG TGAACCACAC CATCCTGCAG TACTATCGCT GCATGGCCAG CGAGCTTGTC
CGTGTCAACA TCACCCCAGT GGTGGCCCTG TGGCAGCCTA TGGCCCCGAA CCAAGGACTG
CCGCGCCTCC TGGCCAGGCA GGGCGCCTGG GAGAACCCCT ACACTGCCCT GGCCTTTGCA
GAGTATGCCC GACTGTGCTT TCAAGAGCTC GGCCATCACG TCAAGCTTTG GATAACGATG
AATGAGCCGT ATACAAGGAA TATGACATAC AGTGCTGGCC ACAACCTTCT GAAGGCCCAT
GCCCTGGCTT GGCATGTGTA CAATGAAAAG TTTAGGCATG CTCAGAATGG GAAAATATCC
ATAGCCTTGC AGGCTGATTG GATAGAACCT GCCTGCCCTT TCTCCCAAAA GGACAAAGAG
GTGGCTGAGA GAGTTTTGGA ATTTGACATT GGCTGGCTGG CTGAGCCCAT TTTCGGCTCT
GGAGATTATC CATGGGTGAT GAGGGACTGG CTGAACCAAA GAAACAATTT TCTTCTTCCT
TATTTCACTG AAGATGAAAA AAAGCTAATC CAGGGTACCT TTGACTTTTT GGCTTTAAGC
CATTATACCA CCATCCTTGT AGACTCAGAA AAAGAAGATC CAATAAAATA CAATGATTAC
CTAGAAGTGC AAGAAATGAC CGACATCACG TGGCTCAACT CCCCCAGTCA GGTGGCGGTA
GTGCCCTGGG GGTTGCGCAA AGTGCTGAAC TGGCTGAAGT TCAAGTACGG AGACCTCCCC
ATGTACATAA TATCCAATGG AATCGATGAC GGGCTGCATG CTGAGGACGA CCAGCTGAGG
GTGTATTATA TGCAGAATTA CATAAACGAA GCTCTCAAAG CCCACATACT GGATGGTATC
AATCTTTGCG GATACTTTGC TTATTCGTTT AACGACCGCA CAGCTCCGAG GTTTGGCCTC
TATCGTTATG CTGCAGATCA GTTTGAGCCC AAGGCATCCA TGAAACATTA CAGGAAAATT
ATTGACAGCA ATGGTTTCCC GGGCCCAGAA ACTCTGGAAA GATTTTGTCC AGAAGAATTC
ACCGTGTGTA CTGAGTGCAG TTTTTTTCAC ACCCGAAAGT CTTTACTGGC TTTCATAGCT
TTTCTATTTT TTGCTTCTAT TATTTCTCTC TCCCTTATAT TTTACTACTC GAAGAAAGGC
AGAAGAAGTT ACAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006719958.1
CDS354..2471
Misc Feature(1)<369..950
Misc Feature(2)981..2291
Translation

Target ORF information:

RefSeq Version XM_006719895.2
Organism Homo sapiens(Human)
Definition Homo sapiens klotho (KL), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006719895.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGACCGACC ACAGCATCAA AGAATGTCAA AAATCTCTGG ACTTTGTACT AGGTTGGTTT 
GCCAAACCCG TATTTATTGA TGGTGACTAT CCCGAGAGCA TGAAGAATAA CCTTTCATCT
ATTCTGCCTG ATTTTACTGA ATCTGAGAAA AAGTTCATCA AAGGAACTGC TGACTTTTTT
GCTCTTTGCT TTGGACCCAC CTTGAGTTTT CAACTTTTGG ACCCTCACAT GAAGTTCCGC
CAATTGGAAT CTCCCAACCT GAGGCAACTG CTTTCCTGGA TTGACCTTGA ATTTAACCAT
CCTCAAATAT TTATTGTGGA AAATGGCTGG TTTGTCTCAG GGACCACCAA GAGAGATGAT
GCCAAATATA TGTATTACCT CAAAAAGTTC ATCATGGAAA CCTTAAAAGC CATCAAGCTG
GATGGGGTGG ATGTCATCGG GTATACCGCA TGGTCCCTCA TGGATGGTTT CGAGTGGCAC
AGAGGTTACA GCATCAGGCG TGGACTCTTC TATGTTGACT TTCTAAGCCA GGACAAGATG
TTGTTGCCAA AGTCTTCAGC CTTGTTCTAC CAAAAGCTGA TAGAGAAAAA TGGCTTCCCT
CCTTTACCTG AAAATCAGCC CCTAGAAGGG ACATTTCCCT GTGACTTTGC TTGGGGAGTT
GTTGACAACT ACATTCAAGT AGATACCACT CTGTCTCAGT TTACCGACCT GAATGTTTAC
CTGTGGGATG TCCACCACAG TAAAAGGCTT ATTAAAGTGG ATGGGGTTGT GACCAAGAAG
AGGAAATCCT ACTGTGTTGA CTTTGCTGCC ATCCAGCCCC AGATCGCTTT ACTCCAGGAA
ATGCACGTTA CACATTTTCG CTTCTCCCTG GACTGGGCCC TGATTCTCCC TCTGGGTAAC
CAGTCCCAGG TGAACCACAC CATCCTGCAG TACTATCGCT GCATGGCCAG CGAGCTTGTC
CGTGTCAACA TCACCCCAGT GGTGGCCCTG TGGCAGCCTA TGGCCCCGAA CCAAGGACTG
CCGCGCCTCC TGGCCAGGCA GGGCGCCTGG GAGAACCCCT ACACTGCCCT GGCCTTTGCA
GAGTATGCCC GACTGTGCTT TCAAGAGCTC GGCCATCACG TCAAGCTTTG GATAACGATG
AATGAGCCGT ATACAAGGAA TATGACATAC AGTGCTGGCC ACAACCTTCT GAAGGCCCAT
GCCCTGGCTT GGCATGTGTA CAATGAAAAG TTTAGGCATG CTCAGAATGG GAAAATATCC
ATAGCCTTGC AGGCTGATTG GATAGAACCT GCCTGCCCTT TCTCCCAAAA GGACAAAGAG
GTGGCTGAGA GAGTTTTGGA ATTTGACATT GGCTGGCTGG CTGAGCCCAT TTTCGGCTCT
GGAGATTATC CATGGGTGAT GAGGGACTGG CTGAACCAAA GAAACAATTT TCTTCTTCCT
TATTTCACTG AAGATGAAAA AAAGCTAATC CAGGGTACCT TTGACTTTTT GGCTTTAAGC
CATTATACCA CCATCCTTGT AGACTCAGAA AAAGAAGATC CAATAAAATA CAATGATTAC
CTAGAAGTGC AAGAAATGAC CGACATCACG TGGCTCAACT CCCCCAGTCA GGTGGCGGTA
GTGCCCTGGG GGTTGCGCAA AGTGCTGAAC TGGCTGAAGT TCAAGTACGG AGACCTCCCC
ATGTACATAA TATCCAATGG AATCGATGAC GGGCTGCATG CTGAGGACGA CCAGCTGAGG
GTGTATTATA TGCAGAATTA CATAAACGAA GCTCTCAAAG CCCACATACT GGATGGTATC
AATCTTTGCG GATACTTTGC TTATTCGTTT AACGACCGCA CAGCTCCGAG GTTTGGCCTC
TATCGTTATG CTGCAGATCA GTTTGAGCCC AAGGCATCCA TGAAACATTA CAGGAAAATT
ATTGACAGCA ATGGTTTCCC GGGCCCAGAA ACTCTGGAAA GATTTTGTCC AGAAGAATTC
ACCGTGTGTA CTGAGTGCAG TTTTTTTCAC ACCCGAAAGT CTTTACTGGC TTTCATAGCT
TTTCTATTTT TTGCTTCTAT TATTTCTCTC TCCCTTATAT TTTACTACTC GAAGAAAGGC
AGAAGAAGTT ACAAATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Association Study of Klotho Gene Polymorphism With Calcium Oxalate Stones in The Uyghur Population of Xinjiang, China.
Urology journal14(1)2939-2943(2017 Jan)
Ali A,Tursun H,Talat A,Abla A,Muhtar E,Zhang T,Mahmut M


book

Decreased Level of Klotho Contributes to Drug Resistance in Lung Cancer Cells: Involving in Klotho-Mediated Cell Autophagy.
DNA and cell biology35(12)751-757(2016 Dec)
Chen T,Ren H,Thakur A,Yang T,Li Y,Zhang S,Wang T,Chen M


book

Clinical significance of α‑ and β‑Klotho in urothelial carcinoma of the bladder.
Oncology reports36(4)2117-25(2016 Oct)
Hori S,Miyake M,Onishi S,Tatsumi Y,Morizawa Y,Nakai Y,Anai S,Tanaka N,Fujimoto K


book

Soluble α-Klotho - a new marker of acromegaly?
Endokrynologia Polska67(4)390-6(2016)
Jawiarczyk-Przybyłowska A,Halupczok-Żyła J,Bolanowski M


book

Changes in Serum Concentrations of Fibroblast Growth Factor 23 and Soluble Klotho in Hemodialysis Patients after Total Parathyroidectomy.
BioMed research international20166453803(2016)
Liao SC,Moi SH,Chou FF,Yang CH,Chen JB


 
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