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PCYT1B cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol PCYT1B
Entrez Gene ID 9468
Full Name phosphate cytidylyltransferase 1, choline, beta
Synonyms CCTB,CTB
General protein information
Preferred Names

phosphate cytidylyltransferase 1, choline, beta

Names

choline-phosphate cytidylyltransferase B
CCT B
CCT-beta
CT B
CTP:phosphocholine cytidylyltransferase b
phosphorylcholine transferase B
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

X

Xp22.11
Summary The protein encoded by this gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009].
Disorder MIM:

300948


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001163265.1 NP_001156737.1 choline-phosphate cytidylyltransferase B isoform 3
NM_001163264.2 NP_001156736.1 choline-phosphate cytidylyltransferase B isoform 2
NM_001163265.2 NP_001156737.1 choline-phosphate cytidylyltransferase B isoform 3
XM_017029977.1 XP_016885466.1 choline-phosphate cytidylyltransferase B isoform X1
NM_004845.4 NP_004836.2 choline-phosphate cytidylyltransferase B isoform 1
NM_001163264.1 NP_001156736.1 choline-phosphate cytidylyltransferase B isoform 2
NM_004845.5 NP_004836.2 choline-phosphate cytidylyltransferase B isoform 1

Pathways from BioSystems


Homologies


Bos taurus (cattle) PCYT1B NP_001179980.1
Oryza sativa (rice) Os10g0578900 NP_001065503.1
Xenopus tropicalis (tropical clawed frog) pcyt1b XP_004911814.1
Homo sapiens (human) PCYT1B NP_004836.2
Canis lupus familiaris (dog) PCYT1B XP_548900.2
Rattus norvegicus (Norway rat) Pcyt1b NP_775174.1
Danio rerio (zebrafish) pcyt1ba NP_001076415.1
Mus musculus (house mouse) Pcyt1b NP_997593.1
Oryza sativa (rice) Os08g0161800 NP_001061052.1
PCYT1B XP_001090055.2
Gallus gallus (chicken) PCYT1B XP_416793.4

Gene Ontology


Bibliography

Related articles in PubMed

CTP:phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis.
Cornell RB, Ridgway ND
Progress in lipid research59147-71(2015 Jul)

Neurokinin 3 receptor and phosphocholine transferase: missing factors for pathogenesis of C-reactive protein in preeclampsia.
Parchim NF, Wang W, Iriyama T, Ashimi OA, Siddiqui AH, Blackwell S, Sibai B, Kellems RE, Xia Y
Hypertension (Dallas, Tex. : 1979)65(2)430-9(2015 Feb)

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

Mass spectrometric identification of human phosphate cytidylyltransferase 1 as a novel calcium oxalate crystal growth inhibitor purified from human renal stone matrix.
Priyadarshini, Singh SK, Tandon C
Clinica chimica acta; international journal of clinical chemistry408(1-2)34-8(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CCT contributes to phospholipid compositional homeostasis. [Review]
Title: CTP:phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis.

PCYT1B, a placental-specific enzyme post-translationally modifying neurokinin B, is essential for the pathogenic role of CRP in preeclampsia through activation of the neurokinin 3 receptor.
Title: Neurokinin 3 receptor and phosphocholine transferase: missing factors for pathogenesis of C-reactive protein in preeclampsia.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Human phosphate cytidylyltransferase 1, choline, beta is a novel CaOx crystal growth inhibitor.
Title: Mass spectrometric identification of human phosphate cytidylyltransferase 1 as a novel calcium oxalate crystal growth inhibitor purified from human renal stone matrix.

The following PCYT1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCYT1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03331 NM_001163264.2
Latest version!		 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu03333 NM_001163265.2
Latest version!		 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu17852 NM_004845.5
Latest version!		 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu99543 XM_017029977.1
Latest version!		 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu03333 NM_001163265.1 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu03331 NM_001163264.1 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu17852 NM_004845.4 Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu03331
Clone ID Related Accession (Same CDS sequence) NM_001163264.2 , NM_001163264.1
Accession Version NM_001163264.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-31
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079168.22, AK226121.1 and BC045634.1. On Jun 1, 2019 this sequence version replaced NM_001163264.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in the use of a different start codon, and a shorter isoform (2) with a distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC045634.1, AK226121.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGTAGGCC ATCAGGAGTG CATCATGGAA GAGGACAATC GCGCTCCCCA ATTGTGGCGC 
AAGACCCTGA CTGCACCTGC CCCATTTGCT GATGAAACCA ACTGCCAGTG TCAAGCACCC 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACAC CAGCTGACAG GCCTGTCAGA 
GTATACGCCG ATGGAATATT TGACCTCTTC CACTCAGGTC ATGCAAGAGC CCTTATGCAA 
GCAAAAACAC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGATCTCACC 
CACAAATTCA AAGGTTTCAC CGTGATGAAT GAAGCCGAGA GATACGAAGC TCTCAGACAC 
TGTCGCTACG TAGACGAAGT TATCAGAGAT GCTCCCTGGA CACTCACGCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAT GATGACATTC CGTATTCCTC TGCTGGCTCT 
GATGATGTTT ACAAGCACAT AAAGGAAGCA GGGATGTTCG TTCCAACGCA GAGAACAGAA 
GGCATCTCAA CATCGGACAT CATTACCAGA ATTGTTCGTG ACTATGATGT TTATGCCCGA 
CGTAACCTCC AGAGAGGGTA TACAGCCAAG GAACTGAATG TCAGCTTTAT AAATGAGAAG 
AGGTACCGTT TCCAGAACCA AGTGGACAAA ATGAAGGAAA AAGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTGAA CAGAGTGGAA GAAAAGAGCC ATGATCTAAT TCAAAAGTGG 
GAAGAGAAGT CAAGGGAATT CATTGGCAAC TTCCTAGAAC TGTTTGGACC TGATGGAGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGCAGC CGGATGCTGC AGGCCTTATC CCCGAAGCAG 
AGCCCTGTGA GCAGCCCAAC CCGGAGCCGG TCCCCTTCCC GCTCCCCATC GCCCACCTTC 
TCATGGCTTC CACTCAAAAC CTCACCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCTATC 
AGCAGCATGA GCGAGGGGGA TGAGGATGAA AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156736.1
CDS256..1311
Translation

Target ORF information:

RefSeq Version NM_001163264.2
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001163264.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGTAGGCC ATCAGGAGTG CATCATGGAA GAGGACAATC GCGCTCCCCA ATTGTGGCGC 
AAGACCCTGA CTGCACCTGC CCCATTTGCT GATGAAACCA ACTGCCAGTG TCAAGCACCC 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACAC CAGCTGACAG GCCTGTCAGA 
GTATACGCCG ATGGAATATT TGACCTCTTC CACTCAGGTC ATGCAAGAGC CCTTATGCAA 
GCAAAAACAC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGATCTCACC 
CACAAATTCA AAGGTTTCAC CGTGATGAAT GAAGCCGAGA GATACGAAGC TCTCAGACAC 
TGTCGCTACG TAGACGAAGT TATCAGAGAT GCTCCCTGGA CACTCACGCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAT GATGACATTC CGTATTCCTC TGCTGGCTCT 
GATGATGTTT ACAAGCACAT AAAGGAAGCA GGGATGTTCG TTCCAACGCA GAGAACAGAA 
GGCATCTCAA CATCGGACAT CATTACCAGA ATTGTTCGTG ACTATGATGT TTATGCCCGA 
CGTAACCTCC AGAGAGGGTA TACAGCCAAG GAACTGAATG TCAGCTTTAT AAATGAGAAG 
AGGTACCGTT TCCAGAACCA AGTGGACAAA ATGAAGGAAA AAGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTGAA CAGAGTGGAA GAAAAGAGCC ATGATCTAAT TCAAAAGTGG 
GAAGAGAAGT CAAGGGAATT CATTGGCAAC TTCCTAGAAC TGTTTGGACC TGATGGAGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGCAGC CGGATGCTGC AGGCCTTATC CCCGAAGCAG 
AGCCCTGTGA GCAGCCCAAC CCGGAGCCGG TCCCCTTCCC GCTCCCCATC GCCCACCTTC 
TCATGGCTTC CACTCAAAAC CTCACCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCTATC 
AGCAGCATGA GCGAGGGGGA TGAGGATGAA AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03331
Clone ID Related Accession (Same CDS sequence) NM_001163264.2 , NM_001163264.1
Accession Version NM_001163264.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1056bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-06-23
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK226121.1 and BC045634.1. Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in the use of a different start codon, and a shorter isoform (2) with a distinct N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK226121.1, SRR1803613.271066.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGTAGGCC ATCAGGAGTG CATCATGGAA GAGGACAATC GCGCTCCCCA ATTGTGGCGC 
AAGACCCTGA CTGCACCTGC CCCATTTGCT GATGAAACCA ACTGCCAGTG TCAAGCACCC 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACAC CAGCTGACAG GCCTGTCAGA 
GTATACGCCG ATGGAATATT TGACCTCTTC CACTCAGGTC ATGCAAGAGC CCTTATGCAA 
GCAAAAACAC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGATCTCACC 
CACAAATTCA AAGGTTTCAC CGTGATGAAT GAAGCCGAGA GATACGAAGC TCTCAGACAC 
TGTCGCTACG TAGACGAAGT TATCAGAGAT GCTCCCTGGA CACTCACGCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAT GATGACATTC CGTATTCCTC TGCTGGCTCT 
GATGATGTTT ACAAGCACAT AAAGGAAGCA GGGATGTTCG TTCCAACGCA GAGAACAGAA 
GGCATCTCAA CATCGGACAT CATTACCAGA ATTGTTCGTG ACTATGATGT TTATGCCCGA 
CGTAACCTCC AGAGAGGGTA TACAGCCAAG GAACTGAATG TCAGCTTTAT AAATGAGAAG 
AGGTACCGTT TCCAGAACCA AGTGGACAAA ATGAAGGAAA AAGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTGAA CAGAGTGGAA GAAAAGAGCC ATGATCTAAT TCAAAAGTGG 
GAAGAGAAGT CAAGGGAATT CATTGGCAAC TTCCTAGAAC TGTTTGGACC TGATGGAGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGCAGC CGGATGCTGC AGGCCTTATC CCCGAAGCAG 
AGCCCTGTGA GCAGCCCAAC CCGGAGCCGG TCCCCTTCCC GCTCCCCATC GCCCACCTTC 
TCATGGCTTC CACTCAAAAC CTCACCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCTATC 
AGCAGCATGA GCGAGGGGGA TGAGGATGAA AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156736.1
CDS231..1286
Translation

Target ORF information:

RefSeq Version NM_001163264.1
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001163264.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGTAGGCC ATCAGGAGTG CATCATGGAA GAGGACAATC GCGCTCCCCA ATTGTGGCGC 
AAGACCCTGA CTGCACCTGC CCCATTTGCT GATGAAACCA ACTGCCAGTG TCAAGCACCC 
CATGAAAAAC TGACCATTGC TCAGGCCCGC TTAGGAACAC CAGCTGACAG GCCTGTCAGA 
GTATACGCCG ATGGAATATT TGACCTCTTC CACTCAGGTC ATGCAAGAGC CCTTATGCAA 
GCAAAAACAC TGTTTCCCAA CAGCTACTTG TTGGTAGGAG TTTGCAGTGA TGATCTCACC 
CACAAATTCA AAGGTTTCAC CGTGATGAAT GAAGCCGAGA GATACGAAGC TCTCAGACAC 
TGTCGCTACG TAGACGAAGT TATCAGAGAT GCTCCCTGGA CACTCACGCC AGAGTTTCTG 
GAAAAACACA AGATTGACTT TGTGGCTCAT GATGACATTC CGTATTCCTC TGCTGGCTCT 
GATGATGTTT ACAAGCACAT AAAGGAAGCA GGGATGTTCG TTCCAACGCA GAGAACAGAA 
GGCATCTCAA CATCGGACAT CATTACCAGA ATTGTTCGTG ACTATGATGT TTATGCCCGA 
CGTAACCTCC AGAGAGGGTA TACAGCCAAG GAACTGAATG TCAGCTTTAT AAATGAGAAG 
AGGTACCGTT TCCAGAACCA AGTGGACAAA ATGAAGGAAA AAGTCAAGAA TGTGGAGGAA 
AGATCAAAGG AATTTGTGAA CAGAGTGGAA GAAAAGAGCC ATGATCTAAT TCAAAAGTGG 
GAAGAGAAGT CAAGGGAATT CATTGGCAAC TTCCTAGAAC TGTTTGGACC TGATGGAGCA 
TGGAAGCAGA TGTTCCAGGA GAGGAGCAGC CGGATGCTGC AGGCCTTATC CCCGAAGCAG 
AGCCCTGTGA GCAGCCCAAC CCGGAGCCGG TCCCCTTCCC GCTCCCCATC GCCCACCTTC 
TCATGGCTTC CACTCAAAAC CTCACCCCCT TCCTCACCCA AAGCAGCCTC AGCCTCTATC 
AGCAGCATGA GCGAGGGGGA TGAGGATGAA AAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03333
Clone ID Related Accession (Same CDS sequence) NM_001163265.1 , NM_001163265.2
Accession Version NM_001163265.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 993bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-21
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315323.1, AF052510.1, AK226121.1 and BC045634.1. Transcript Variant: This variant (3) is alternatively spliced at the 3' end compared to variant 1, resulting in a frameshift and a shorter isoform (3) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF052510.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
CTGAAGAGTT GGGCGAGGTG CAGAGACTTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156737.1
CDS234..1226
Translation

Target ORF information:

RefSeq Version NM_001163265.1
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001163265.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
CTGAAGAGTT GGGCGAGGTG CAGAGACTTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu03333
Clone ID Related Accession (Same CDS sequence) NM_001163265.1 , NM_001163265.2
Accession Version NM_001163265.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 993bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-05-30
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform 3
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079168.22, AK315323.1, AF052510.1, AK226121.1 and BC045634.1. On May 31, 2019 this sequence version replaced NM_001163265.1. Transcript Variant: This variant (3) is alternatively spliced at the 3' end compared to variant 1, resulting in a frameshift and a shorter isoform (3) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AF052510.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
CTGAAGAGTT GGGCGAGGTG CAGAGACTTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001156737.1
CDS243..1235
Translation

Target ORF information:

RefSeq Version NM_001163265.2
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001163265.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
CTGAAGAGTT GGGCGAGGTG CAGAGACTTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17852
Clone ID Related Accession (Same CDS sequence) NM_004845.4 , NM_004845.5
Accession Version NM_004845.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-21
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315323.1, AK226121.1 and BC045634.1. This sequence is a reference standard in the RefSeqGene project. On Jul 14, 2009 this sequence version replaced NM_004845.3. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK315323.1, SRR1660805.22004.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
GTGAGCAGCC CAACCCGGAG CCGGTCCCCT TCCCGCTCCC CATCGCCCAC CTTCTCATGG 
CTTCCACTCA AAACCTCACC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC TATCAGCAGC 
ATGAGCGAGG GGGATGAGGA TGAAAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004836.2
CDS234..1343
Translation

Target ORF information:

RefSeq Version NM_004845.4
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_004845.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
GTGAGCAGCC CAACCCGGAG CCGGTCCCCT TCCCGCTCCC CATCGCCCAC CTTCTCATGG 
CTTCCACTCA AAACCTCACC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC TATCAGCAGC 
ATGAGCGAGG GGGATGAGGA TGAAAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17852
Clone ID Related Accession (Same CDS sequence) NM_004845.4 , NM_004845.5
Accession Version NM_004845.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1110bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-25
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC079168.22, AK315323.1, AK226121.1 and BC045634.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004845.4. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.59370.1, SRR1660805.104310.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379144.7/ ENSP00000368439.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
GTGAGCAGCC CAACCCGGAG CCGGTCCCCT TCCCGCTCCC CATCGCCCAC CTTCTCATGG 
CTTCCACTCA AAACCTCACC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC TATCAGCAGC 
ATGAGCGAGG GGGATGAGGA TGAAAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004836.2
CDS243..1352
Translation

Target ORF information:

RefSeq Version NM_004845.5
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_004845.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGCCAGTAG TTACCACTGA TGCTGAGTCA GAAACAGGTA TCCCAAAATC CCTTTCCAAT 
GAGCCTCCCT CAGAAACCAT GGAGGAAATA GAGCACACAT GCCCACAGCC TCGACTGACC 
CTGACTGCAC CTGCCCCATT TGCTGATGAA ACCAACTGCC AGTGTCAAGC ACCCCATGAA 
AAACTGACCA TTGCTCAGGC CCGCTTAGGA ACACCAGCTG ACAGGCCTGT CAGAGTATAC 
GCCGATGGAA TATTTGACCT CTTCCACTCA GGTCATGCAA GAGCCCTTAT GCAAGCAAAA 
ACACTGTTTC CCAACAGCTA CTTGTTGGTA GGAGTTTGCA GTGATGATCT CACCCACAAA 
TTCAAAGGTT TCACCGTGAT GAATGAAGCC GAGAGATACG AAGCTCTCAG ACACTGTCGC 
TACGTAGACG AAGTTATCAG AGATGCTCCC TGGACACTCA CGCCAGAGTT TCTGGAAAAA 
CACAAGATTG ACTTTGTGGC TCATGATGAC ATTCCGTATT CCTCTGCTGG CTCTGATGAT 
GTTTACAAGC ACATAAAGGA AGCAGGGATG TTCGTTCCAA CGCAGAGAAC AGAAGGCATC 
TCAACATCGG ACATCATTAC CAGAATTGTT CGTGACTATG ATGTTTATGC CCGACGTAAC 
CTCCAGAGAG GGTATACAGC CAAGGAACTG AATGTCAGCT TTATAAATGA GAAGAGGTAC 
CGTTTCCAGA ACCAAGTGGA CAAAATGAAG GAAAAAGTCA AGAATGTGGA GGAAAGATCA 
AAGGAATTTG TGAACAGAGT GGAAGAAAAG AGCCATGATC TAATTCAAAA GTGGGAAGAG 
AAGTCAAGGG AATTCATTGG CAACTTCCTA GAACTGTTTG GACCTGATGG AGCATGGAAG 
CAGATGTTCC AGGAGAGGAG CAGCCGGATG CTGCAGGCCT TATCCCCGAA GCAGAGCCCT 
GTGAGCAGCC CAACCCGGAG CCGGTCCCCT TCCCGCTCCC CATCGCCCAC CTTCTCATGG 
CTTCCACTCA AAACCTCACC CCCTTCCTCA CCCAAAGCAG CCTCAGCCTC TATCAGCAGC 
ATGAGCGAGG GGGATGAGGA TGAAAAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu99543
Clone ID Related Accession (Same CDS sequence) XM_017029977.1
Accession Version XM_017029977.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product choline-phosphate cytidylyltransferase B isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAAGCAA AAACACTGTT TCCCAACAGC TACTTGTTGG TAGGAGTTTG CAGTGATGAT 
CTCACCCACA AATTCAAAGG TTTCACCGTG ATGAATGAAG CCGAGAGATA CGAAGCTCTC 
AGACACTGTC GCTACGTAGA CGAAGTTATC AGAGATGCTC CCTGGACACT CACGCCAGAG 
TTTCTGGAAA AACACAAGAT TGACTTTGTG GCTCATGATG ACATTCCGTA TTCCTCTGCT 
GGCTCTGATG ATGTTTACAA GCACATAAAG GAAGCAGGGA TGTTCGTTCC AACGCAGAGA 
ACAGAAGGCA TCTCAACATC GGACATCATT ACCAGAATTG TTCGTGACTA TGATGTTTAT 
GCCCGACGTA ACCTCCAGAG AGGGTATACA GCCAAGGAAC TGAATGTCAG CTTTATAAAT 
GAGAAGAGGT ACCGTTTCCA GAACCAAGTG GACAAAATGA AGGAAAAAGT CAAGAATGTG 
GAGGAAAGAT CAAAGGAATT TGTGAACAGA GTGGAAGAAA AGAGCCATGA TCTAATTCAA 
AAGTGGGAAG AGAAGTCAAG GGAATTCATT GGCAACTTCC TAGAACTGTT TGGACCTGAT 
GGAGCATGGA AGCAGATGTT CCAGGAGAGG AGCAGCCGGA TGCTGCAGGC CTTATCCCCG 
AAGCAGAGCC CTGTGAGCAG CCCAACCCGG AGCCGGTCCC CTTCCCGCTC CCCATCGCCC 
ACCTTCTCAT GGCTTCCACT CAAAACCTCA CCCCCTTCCT CACCCAAAGC AGCCTCAGCC 
TCTATCAGCA GCATGAGCGA GGGGGATGAG GATGAAAAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016885466.1
CDS347..1168
Translation

Target ORF information:

RefSeq Version XM_017029977.1
Organism Homo sapiens(human)
Definition Homo sapiens phosphate cytidylyltransferase 1, choline, beta (PCYT1B), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017029977.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAAGCAA AAACACTGTT TCCCAACAGC TACTTGTTGG TAGGAGTTTG CAGTGATGAT 
CTCACCCACA AATTCAAAGG TTTCACCGTG ATGAATGAAG CCGAGAGATA CGAAGCTCTC 
AGACACTGTC GCTACGTAGA CGAAGTTATC AGAGATGCTC CCTGGACACT CACGCCAGAG 
TTTCTGGAAA AACACAAGAT TGACTTTGTG GCTCATGATG ACATTCCGTA TTCCTCTGCT 
GGCTCTGATG ATGTTTACAA GCACATAAAG GAAGCAGGGA TGTTCGTTCC AACGCAGAGA 
ACAGAAGGCA TCTCAACATC GGACATCATT ACCAGAATTG TTCGTGACTA TGATGTTTAT 
GCCCGACGTA ACCTCCAGAG AGGGTATACA GCCAAGGAAC TGAATGTCAG CTTTATAAAT 
GAGAAGAGGT ACCGTTTCCA GAACCAAGTG GACAAAATGA AGGAAAAAGT CAAGAATGTG 
GAGGAAAGAT CAAAGGAATT TGTGAACAGA GTGGAAGAAA AGAGCCATGA TCTAATTCAA 
AAGTGGGAAG AGAAGTCAAG GGAATTCATT GGCAACTTCC TAGAACTGTT TGGACCTGAT 
GGAGCATGGA AGCAGATGTT CCAGGAGAGG AGCAGCCGGA TGCTGCAGGC CTTATCCCCG 
AAGCAGAGCC CTGTGAGCAG CCCAACCCGG AGCCGGTCCC CTTCCCGCTC CCCATCGCCC 
ACCTTCTCAT GGCTTCCACT CAAAACCTCA CCCCCTTCCT CACCCAAAGC AGCCTCAGCC 
TCTATCAGCA GCATGAGCGA GGGGGATGAG GATGAAAAGT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

CTP:phosphocholine cytidylyltransferase: Function, regulation, and structure of an amphitropic enzyme required for membrane biogenesis.
Progress in lipid research59147-71(2015 Jul)
Cornell RB,Ridgway ND


book

Neurokinin 3 receptor and phosphocholine transferase: missing factors for pathogenesis of C-reactive protein in preeclampsia.
Hypertension (Dallas, Tex. : 1979)65(2)430-9(2015 Feb)
Parchim NF,Wang W,Iriyama T,Ashimi OA,Siddiqui AH,Blackwell S,Sibai B,Kellems RE,Xia Y


book

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,


book

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,


book

Mass spectrometric identification of human phosphate cytidylyltransferase 1 as a novel calcium oxalate crystal growth inhibitor purified from human renal stone matrix.
Clinica chimica acta; international journal of clinical chemistry408(1-2)34-8(2009 Oct)
Priyadarshini,Singh SK,Tandon C