PITPNM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PITPNM1
Entrez Gene ID	9600
Full Name	phosphatidylinositol transfer protein membrane associated 1
Synonyms	DRES9,NIR2,PITPNM,RDGB,RDGB1,RDGBA,RDGBA1,Rd9
General protein information	Preferred Names phosphatidylinositol transfer protein membrane associated 1 Names membrane-associated phosphatidylinositol transfer protein 1 NIR-2 PITPnm 1 PYK2 N-terminal domain-interacting receptor 2 drosophila retinal degeneration B homolog retinal degeneration B alpha 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.2
Summary	PITPNM1 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al., 2005 [PubMed 15627748]).[supplied by OMIM, Mar 2008].
Disorder MIM:	608794

mRNA and Protein(s)

mRNA	Protein	Name
XM_011545396.2	XP_011543698.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
XM_017018586.1	XP_016874075.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X1
NM_001130848.2	NP_001124320.1	membrane-associated phosphatidylinositol transfer protein 1 isoform b
NM_004910.3	NP_004901.2	membrane-associated phosphatidylinositol transfer protein 1 isoform a
XM_011545396.3	XP_011543698.1	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
NM_001130848.1	NP_001124320.1	membrane-associated phosphatidylinositol transfer protein 1 isoform b
NM_004910.2	NP_004901.2	membrane-associated phosphatidylinositol transfer protein 1 isoform a

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pitpnm1	NP_032877.1
Rattus norvegicus (Norway rat)	Pitpnm1	NP_001008370.1
Homo sapiens (human)	PITPNM1	NP_004901.2
Pan troglodytes (chimpanzee)	PITPNM1	XP_003313225.1
	PITPNM1	XP_001117918.1
Gallus gallus (chicken)	PITPNM1	XP_001233221.3
Canis lupus familiaris (dog)	PITPNM1	XP_851829.1
Bos taurus (cattle)	PITPNM1	XP_002699412.1

Gene Ontology
Bibliography

Related articles in PubMed

Phosphatidylinositol 4,5-Bisphosphate Homeostasis Regulated by Nir2 and Nir3 Proteins at Endoplasmic Reticulum-Plasma Membrane Junctions.
Chang CL, Liou J
The Journal of biological chemistry290(23)14289-301(2015 Jun)

The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis.
Keinan O, Kedan A, Gavert N, Selitrennik M, Kim S, Karn T, Becker S, Lev S
Journal of cell science127(Pt 21)4740-9(2014 Nov)

Feedback regulation of receptor-induced Ca2+ signaling mediated by E-Syt1 and Nir2 at endoplasmic reticulum-plasma membrane junctions.
Chang CL, Hsieh TS, Yang TT, Rothberg KG, Azizoglu DB, Volk E, Liao JC, Liou J
Cell reports5(3)813-25(2013 Nov)

The phosphatidylinositol-transfer protein Nir2 binds phosphatidic acid and positively regulates phosphoinositide signalling.
Kim S, Kedan A, Marom M, Gavert N, Keinan O, Selitrennik M, Laufman O, Lev S
EMBO reports14(10)891-9(2013 Oct)

Phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) binds and transfers phosphatidic acid.
Garner K, Hunt AN, Koster G, Somerharju P, Groves E, Li M, Raghu P, Holic R, Cockcroft S
The Journal of biological chemistry287(38)32263-76(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphatidic acid production triggers Phosphatidylinositol 4,5-Bisphosphate replenishment mediated by Nir2 and Nir3 at endoplasmic reticulum-plasma membrane junctions.
Title: Phosphatidylinositol 4,5-Bisphosphate Homeostasis Regulated by Nir2 and Nir3 Proteins at Endoplasmic Reticulum-Plasma Membrane Junctions.

results suggest that Nir2 not only enhances EMT in vitro and breast cancer metastasis in animal models, but also contributes to breast cancer progression in human patients.
Title: The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis.

Results suggest a feedback mechanism that replenishes PM PIP2 during receptor-induced Ca(2+) signaling via the Ca(2+) effector E-Syt1 and the PITP Nir2 at ER-PM junctions.
Title: Feedback regulation of receptor-induced Ca2+ signaling mediated by E-Syt1 and Nir2 at endoplasmic reticulum-plasma membrane junctions.

Nir2 translocates from the Golgi complex to the plasma membrane in response to GF stimulation.
Title: The phosphatidylinositol-transfer protein Nir2 binds phosphatidic acid and positively regulates phosphoinositide signalling.

RdgB homologs play a preemptive role in excluding endogenous and exogenous modified purine deoxyribonucleoside triphosphates (dTNPs) from incorporation into DNA.
Title: Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase.

The following PITPNM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PITPNM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04677	NM_004910.3 Latest version!	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu07087	NM_001130848.2 Latest version!	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu04677	XM_017018586.1 Latest version!	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu62963	XM_011545396.3 Latest version!	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu62963	XM_011545396.2	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu07087	NM_001130848.1	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu04677	NM_004910.2	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PITPNM1 ( NM_004910.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004901.2 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04677
Clone ID Related Accession (Same CDS sequence)	XM_017018586.1 , NM_004910.3 , NM_004910.2
Accession Version	NM_004910.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA318858.1, AF334584.1, AA465501.1, AK312886.1 and X98654.1. On Nov 23, 2018 this sequence version replaced NM_004910.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF334584.1, SRR1660805.203889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356404.8/ ENSP00000348772.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCAGC CCAGATGCGC 
CCAGCCTGTG AACAGATCTA CAACCTCTTC CACGCGGCTG ACCCCTGCGC CTCACGCCTC 
GAGCCCCTGC TGGCCCCGAA GTTCCAGGCC ATCGCCCCAC TGACCGTGCC CCGCTACCAG 
AAGTTCCCCC TGGGAGATGG CTCATCCCTG CTGCTGGCCG ACACTCTGCA GACGCACTCC 
AGCCTCTTTC TGGAGGAGCT GGAGATGCTG GTGCCCTCAA CACCCACCTC TACTAGCGGT 
GCCTTCTGGA AGGGCAGTGA GTTGGCCACT GACCCCCCGG CCCAGCCAGC CGCCCCCAGC 
ACCACCAGTG AGGTGGTTAA GATCCTGGAG CGCTGGTGGG GGACCAAGCG GATCGACTAC 
TCGCTGTACT GCCCCGAGGC GCTCACCGCC TTTCCCACCG TCACGCTGCC CCACCTCTTC 
CACGCCAGCT ACTGGGAGTC CGCCGACGTG GTGGCGTTCA TCCTGCGCCA GGTGATCGAG 
AAGGAGCGGC CACAGCTGGC GGAATGCGAG GAGCCGTCCA TCTACAGCCC GGCCTTCCCC 
AGGGAGAAGT GGCAGCGAAA ACGCACGCAG GTCAAGATCC GGAACGTCAC TTCCAACCAC 
CGGGCGAGCG ACACGGTGGT GTGCGAGGGC CGCCCCCAGG TGCTAAGCGG GCGCTTCATG 
TACGGGCCCC TGGACGTCGT CACGCTCACT GGAGAGAAGG TGGATGTCTA CATCATGACG 
CAGCCGCTGT CGGGCAAGTG GATCCACTTT GGCACCGAAG TCACCAATAG CTCGGGCCGC 
CTCACCTTCC CAGTTCCCCC AGAACGCGCG CTGGGCATTG GTGTCTACCC CGTGCGCATG 
GTGGTCAGGG GCGACCACAC CTATGCCGAA TGCTGCCTGA CTGTGGTGGC CCGCGGCACG 
GAGGCTGTGG TCTTCAGCAT CGACGGCTCC TTCACCGCCA GCGTCTCCAT CATGGGCAGC 
GACCCCAAGG TGCGAGCTGG CGCCGTGGAC GTGGTCAGGC ACTGGCAGGA CTCCGGCTAC 
CTGATCGTGT ATGTCACAGG CCGGCCGGAT ATGCAGAAGC ACCGCGTGGT GGCATGGCTG 
TCGCAGCACA ACTTCCCCCA CGGCGTCGTC TCCTTCTGCG ACGGCCTCAC CCACGACCCA 
CTACGCCAGA AGGCAATGTT TCTGCAGAGC CTGGTGCAGG AGGTAGAACT GAACATCGTG 
GCCGGTTATG GGTCTCCCAA AGATGTGGCT GTATACGCGG CGCTGGGGCT GTCCCCGAGC 
CAGACCTACA TCGTGGGCCG TGCCGTGCGG AAGCTACAGG CGCAGTGCCA GTTCCTGTCA 
GACGGCTATG TGGCCCACCT GGGCCAGCTG GAAGCGGGCT CGCACTCGCA TGCCTCCTCG 
GGACCCCCGA GAGCTGCCTT GGGCAAGAGC AGCTATGGTG TGGCTGCCCC CGTGGACTTC 
CTGCGCAAAC AGAGCCAGCT GCTTCGCTCG AGGGGCCCCA GCCAGGCGGA GCGTGAGGGC 
CCGGGAACAC CACCCACCAC CCTGGCACGG GGCAAAGCAC GGAGCATCAG CCTGAAGCTG 
GACAGCGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004901.2
CDS	218..3952
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	NM_004910.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004910.3

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCAGC CCAGATGCGC 
CCAGCCTGTG AACAGATCTA CAACCTCTTC CACGCGGCTG ACCCCTGCGC CTCACGCCTC 
GAGCCCCTGC TGGCCCCGAA GTTCCAGGCC ATCGCCCCAC TGACCGTGCC CCGCTACCAG 
AAGTTCCCCC TGGGAGATGG CTCATCCCTG CTGCTGGCCG ACACTCTGCA GACGCACTCC 
AGCCTCTTTC TGGAGGAGCT GGAGATGCTG GTGCCCTCAA CACCCACCTC TACTAGCGGT 
GCCTTCTGGA AGGGCAGTGA GTTGGCCACT GACCCCCCGG CCCAGCCAGC CGCCCCCAGC 
ACCACCAGTG AGGTGGTTAA GATCCTGGAG CGCTGGTGGG GGACCAAGCG GATCGACTAC 
TCGCTGTACT GCCCCGAGGC GCTCACCGCC TTTCCCACCG TCACGCTGCC CCACCTCTTC 
CACGCCAGCT ACTGGGAGTC CGCCGACGTG GTGGCGTTCA TCCTGCGCCA GGTGATCGAG 
AAGGAGCGGC CACAGCTGGC GGAATGCGAG GAGCCGTCCA TCTACAGCCC GGCCTTCCCC 
AGGGAGAAGT GGCAGCGAAA ACGCACGCAG GTCAAGATCC GGAACGTCAC TTCCAACCAC 
CGGGCGAGCG ACACGGTGGT GTGCGAGGGC CGCCCCCAGG TGCTAAGCGG GCGCTTCATG 
TACGGGCCCC TGGACGTCGT CACGCTCACT GGAGAGAAGG TGGATGTCTA CATCATGACG 
CAGCCGCTGT CGGGCAAGTG GATCCACTTT GGCACCGAAG TCACCAATAG CTCGGGCCGC 
CTCACCTTCC CAGTTCCCCC AGAACGCGCG CTGGGCATTG GTGTCTACCC CGTGCGCATG 
GTGGTCAGGG GCGACCACAC CTATGCCGAA TGCTGCCTGA CTGTGGTGGC CCGCGGCACG 
GAGGCTGTGG TCTTCAGCAT CGACGGCTCC TTCACCGCCA GCGTCTCCAT CATGGGCAGC 
GACCCCAAGG TGCGAGCTGG CGCCGTGGAC GTGGTCAGGC ACTGGCAGGA CTCCGGCTAC 
CTGATCGTGT ATGTCACAGG CCGGCCGGAT ATGCAGAAGC ACCGCGTGGT GGCATGGCTG 
TCGCAGCACA ACTTCCCCCA CGGCGTCGTC TCCTTCTGCG ACGGCCTCAC CCACGACCCA 
CTACGCCAGA AGGCAATGTT TCTGCAGAGC CTGGTGCAGG AGGTAGAACT GAACATCGTG 
GCCGGTTATG GGTCTCCCAA AGATGTGGCT GTATACGCGG CGCTGGGGCT GTCCCCGAGC 
CAGACCTACA TCGTGGGCCG TGCCGTGCGG AAGCTACAGG CGCAGTGCCA GTTCCTGTCA 
GACGGCTATG TGGCCCACCT GGGCCAGCTG GAAGCGGGCT CGCACTCGCA TGCCTCCTCG 
GGACCCCCGA GAGCTGCCTT GGGCAAGAGC AGCTATGGTG TGGCTGCCCC CGTGGACTTC 
CTGCGCAAAC AGAGCCAGCT GCTTCGCTCG AGGGGCCCCA GCCAGGCGGA GCGTGAGGGC 
CCGGGAACAC CACCCACCAC CCTGGCACGG GGCAAAGCAC GGAGCATCAG CCTGAAGCTG 
GACAGCGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITPNM1 ( NM_004910.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004901.2 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04677
Clone ID Related Accession (Same CDS sequence)	XM_017018586.1 , NM_004910.3 , NM_004910.2
Accession Version	NM_004910.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA318858.1, AF334584.1, AA465501.1, AK312886.1 and X98654.1. On Aug 6, 2008 this sequence version replaced NM_004910.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF334584.1, SRR1660805.203889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCAGC CCAGATGCGC 
CCAGCCTGTG AACAGATCTA CAACCTCTTC CACGCGGCTG ACCCCTGCGC CTCACGCCTC 
GAGCCCCTGC TGGCCCCGAA GTTCCAGGCC ATCGCCCCAC TGACCGTGCC CCGCTACCAG 
AAGTTCCCCC TGGGAGATGG CTCATCCCTG CTGCTGGCCG ACACTCTGCA GACGCACTCC 
AGCCTCTTTC TGGAGGAGCT GGAGATGCTG GTGCCCTCAA CACCCACCTC TACTAGCGGT 
GCCTTCTGGA AGGGCAGTGA GTTGGCCACT GACCCCCCGG CCCAGCCAGC CGCCCCCAGC 
ACCACCAGTG AGGTGGTTAA GATCCTGGAG CGCTGGTGGG GGACCAAGCG GATCGACTAC 
TCGCTGTACT GCCCCGAGGC GCTCACCGCC TTTCCCACCG TCACGCTGCC CCACCTCTTC 
CACGCCAGCT ACTGGGAGTC CGCCGACGTG GTGGCGTTCA TCCTGCGCCA GGTGATCGAG 
AAGGAGCGGC CACAGCTGGC GGAATGCGAG GAGCCGTCCA TCTACAGCCC GGCCTTCCCC 
AGGGAGAAGT GGCAGCGAAA ACGCACGCAG GTCAAGATCC GGAACGTCAC TTCCAACCAC 
CGGGCGAGCG ACACGGTGGT GTGCGAGGGC CGCCCCCAGG TGCTAAGCGG GCGCTTCATG 
TACGGGCCCC TGGACGTCGT CACGCTCACT GGAGAGAAGG TGGATGTCTA CATCATGACG 
CAGCCGCTGT CGGGCAAGTG GATCCACTTT GGCACCGAAG TCACCAATAG CTCGGGCCGC 
CTCACCTTCC CAGTTCCCCC AGAACGCGCG CTGGGCATTG GTGTCTACCC CGTGCGCATG 
GTGGTCAGGG GCGACCACAC CTATGCCGAA TGCTGCCTGA CTGTGGTGGC CCGCGGCACG 
GAGGCTGTGG TCTTCAGCAT CGACGGCTCC TTCACCGCCA GCGTCTCCAT CATGGGCAGC 
GACCCCAAGG TGCGAGCTGG CGCCGTGGAC GTGGTCAGGC ACTGGCAGGA CTCCGGCTAC 
CTGATCGTGT ATGTCACAGG CCGGCCGGAT ATGCAGAAGC ACCGCGTGGT GGCATGGCTG 
TCGCAGCACA ACTTCCCCCA CGGCGTCGTC TCCTTCTGCG ACGGCCTCAC CCACGACCCA 
CTACGCCAGA AGGCAATGTT TCTGCAGAGC CTGGTGCAGG AGGTAGAACT GAACATCGTG 
GCCGGTTATG GGTCTCCCAA AGATGTGGCT GTATACGCGG CGCTGGGGCT GTCCCCGAGC 
CAGACCTACA TCGTGGGCCG TGCCGTGCGG AAGCTACAGG CGCAGTGCCA GTTCCTGTCA 
GACGGCTATG TGGCCCACCT GGGCCAGCTG GAAGCGGGCT CGCACTCGCA TGCCTCCTCG 
GGACCCCCGA GAGCTGCCTT GGGCAAGAGC AGCTATGGTG TGGCTGCCCC CGTGGACTTC 
CTGCGCAAAC AGAGCCAGCT GCTTCGCTCG AGGGGCCCCA GCCAGGCGGA GCGTGAGGGC 
CCGGGAACAC CACCCACCAC CCTGGCACGG GGCAAAGCAC GGAGCATCAG CCTGAAGCTG 
GACAGCGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004901.2
CDS	227..3961
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	NM_004910.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004910.2

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCAGC CCAGATGCGC 
CCAGCCTGTG AACAGATCTA CAACCTCTTC CACGCGGCTG ACCCCTGCGC CTCACGCCTC 
GAGCCCCTGC TGGCCCCGAA GTTCCAGGCC ATCGCCCCAC TGACCGTGCC CCGCTACCAG 
AAGTTCCCCC TGGGAGATGG CTCATCCCTG CTGCTGGCCG ACACTCTGCA GACGCACTCC 
AGCCTCTTTC TGGAGGAGCT GGAGATGCTG GTGCCCTCAA CACCCACCTC TACTAGCGGT 
GCCTTCTGGA AGGGCAGTGA GTTGGCCACT GACCCCCCGG CCCAGCCAGC CGCCCCCAGC 
ACCACCAGTG AGGTGGTTAA GATCCTGGAG CGCTGGTGGG GGACCAAGCG GATCGACTAC 
TCGCTGTACT GCCCCGAGGC GCTCACCGCC TTTCCCACCG TCACGCTGCC CCACCTCTTC 
CACGCCAGCT ACTGGGAGTC CGCCGACGTG GTGGCGTTCA TCCTGCGCCA GGTGATCGAG 
AAGGAGCGGC CACAGCTGGC GGAATGCGAG GAGCCGTCCA TCTACAGCCC GGCCTTCCCC 
AGGGAGAAGT GGCAGCGAAA ACGCACGCAG GTCAAGATCC GGAACGTCAC TTCCAACCAC 
CGGGCGAGCG ACACGGTGGT GTGCGAGGGC CGCCCCCAGG TGCTAAGCGG GCGCTTCATG 
TACGGGCCCC TGGACGTCGT CACGCTCACT GGAGAGAAGG TGGATGTCTA CATCATGACG 
CAGCCGCTGT CGGGCAAGTG GATCCACTTT GGCACCGAAG TCACCAATAG CTCGGGCCGC 
CTCACCTTCC CAGTTCCCCC AGAACGCGCG CTGGGCATTG GTGTCTACCC CGTGCGCATG 
GTGGTCAGGG GCGACCACAC CTATGCCGAA TGCTGCCTGA CTGTGGTGGC CCGCGGCACG 
GAGGCTGTGG TCTTCAGCAT CGACGGCTCC TTCACCGCCA GCGTCTCCAT CATGGGCAGC 
GACCCCAAGG TGCGAGCTGG CGCCGTGGAC GTGGTCAGGC ACTGGCAGGA CTCCGGCTAC 
CTGATCGTGT ATGTCACAGG CCGGCCGGAT ATGCAGAAGC ACCGCGTGGT GGCATGGCTG 
TCGCAGCACA ACTTCCCCCA CGGCGTCGTC TCCTTCTGCG ACGGCCTCAC CCACGACCCA 
CTACGCCAGA AGGCAATGTT TCTGCAGAGC CTGGTGCAGG AGGTAGAACT GAACATCGTG 
GCCGGTTATG GGTCTCCCAA AGATGTGGCT GTATACGCGG CGCTGGGGCT GTCCCCGAGC 
CAGACCTACA TCGTGGGCCG TGCCGTGCGG AAGCTACAGG CGCAGTGCCA GTTCCTGTCA 
GACGGCTATG TGGCCCACCT GGGCCAGCTG GAAGCGGGCT CGCACTCGCA TGCCTCCTCG 
GGACCCCCGA GAGCTGCCTT GGGCAAGAGC AGCTATGGTG TGGCTGCCCC CGTGGACTTC 
CTGCGCAAAC AGAGCCAGCT GCTTCGCTCG AGGGGCCCCA GCCAGGCGGA GCGTGAGGGC 
CCGGGAACAC CACCCACCAC CCTGGCACGG GGCAAAGCAC GGAGCATCAG CCTGAAGCTG 
GACAGCGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITPNM1 ( NM_001130848.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124320.1 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 2, mRNA.

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CloneID	OHu07087
Clone ID Related Accession (Same CDS sequence)	NM_001130848.2 , NM_001130848.1
Accession Version	NM_001130848.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC022230.1, AA465501.1, AK312886.1 and X98654.1. On May 31, 2019 this sequence version replaced NM_001130848.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is 1 aa shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022230.1, SRR1660809.82186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124320.1
CDS	218..3949
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	NM_001130848.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130848.2

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITPNM1 ( NM_001130848.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124320.1 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 2, mRNA.

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CloneID	OHu07087
Clone ID Related Accession (Same CDS sequence)	NM_001130848.2 , NM_001130848.1
Accession Version	NM_001130848.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA318858.1, BC022230.1, AA465501.1, AK312886.1 and X98654.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the end of an exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is 1 aa shorter compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022230.1, SRR1660809.82186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124320.1
CDS	227..3958
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	NM_001130848.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130848.1

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITPNM1 ( XM_017018586.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874075.1 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X1, mRNA.

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CloneID	OHu04677
Clone ID Related Accession (Same CDS sequence)	XM_017018586.1 , NM_004910.3 , NM_004910.2
Accession Version	XM_017018586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCAGC CCAGATGCGC 
CCAGCCTGTG AACAGATCTA CAACCTCTTC CACGCGGCTG ACCCCTGCGC CTCACGCCTC 
GAGCCCCTGC TGGCCCCGAA GTTCCAGGCC ATCGCCCCAC TGACCGTGCC CCGCTACCAG 
AAGTTCCCCC TGGGAGATGG CTCATCCCTG CTGCTGGCCG ACACTCTGCA GACGCACTCC 
AGCCTCTTTC TGGAGGAGCT GGAGATGCTG GTGCCCTCAA CACCCACCTC TACTAGCGGT 
GCCTTCTGGA AGGGCAGTGA GTTGGCCACT GACCCCCCGG CCCAGCCAGC CGCCCCCAGC 
ACCACCAGTG AGGTGGTTAA GATCCTGGAG CGCTGGTGGG GGACCAAGCG GATCGACTAC 
TCGCTGTACT GCCCCGAGGC GCTCACCGCC TTTCCCACCG TCACGCTGCC CCACCTCTTC 
CACGCCAGCT ACTGGGAGTC CGCCGACGTG GTGGCGTTCA TCCTGCGCCA GGTGATCGAG 
AAGGAGCGGC CACAGCTGGC GGAATGCGAG GAGCCGTCCA TCTACAGCCC GGCCTTCCCC 
AGGGAGAAGT GGCAGCGAAA ACGCACGCAG GTCAAGATCC GGAACGTCAC TTCCAACCAC 
CGGGCGAGCG ACACGGTGGT GTGCGAGGGC CGCCCCCAGG TGCTAAGCGG GCGCTTCATG 
TACGGGCCCC TGGACGTCGT CACGCTCACT GGAGAGAAGG TGGATGTCTA CATCATGACG 
CAGCCGCTGT CGGGCAAGTG GATCCACTTT GGCACCGAAG TCACCAATAG CTCGGGCCGC 
CTCACCTTCC CAGTTCCCCC AGAACGCGCG CTGGGCATTG GTGTCTACCC CGTGCGCATG 
GTGGTCAGGG GCGACCACAC CTATGCCGAA TGCTGCCTGA CTGTGGTGGC CCGCGGCACG 
GAGGCTGTGG TCTTCAGCAT CGACGGCTCC TTCACCGCCA GCGTCTCCAT CATGGGCAGC 
GACCCCAAGG TGCGAGCTGG CGCCGTGGAC GTGGTCAGGC ACTGGCAGGA CTCCGGCTAC 
CTGATCGTGT ATGTCACAGG CCGGCCGGAT ATGCAGAAGC ACCGCGTGGT GGCATGGCTG 
TCGCAGCACA ACTTCCCCCA CGGCGTCGTC TCCTTCTGCG ACGGCCTCAC CCACGACCCA 
CTACGCCAGA AGGCAATGTT TCTGCAGAGC CTGGTGCAGG AGGTAGAACT GAACATCGTG 
GCCGGTTATG GGTCTCCCAA AGATGTGGCT GTATACGCGG CGCTGGGGCT GTCCCCGAGC 
CAGACCTACA TCGTGGGCCG TGCCGTGCGG AAGCTACAGG CGCAGTGCCA GTTCCTGTCA 
GACGGCTATG TGGCCCACCT GGGCCAGCTG GAAGCGGGCT CGCACTCGCA TGCCTCCTCG 
GGACCCCCGA GAGCTGCCTT GGGCAAGAGC AGCTATGGTG TGGCTGCCCC CGTGGACTTC 
CTGCGCAAAC AGAGCCAGCT GCTTCGCTCG AGGGGCCCCA GCCAGGCGGA GCGTGAGGGC 
CCGGGAACAC CACCCACCAC CCTGGCACGG GGCAAAGCAC GGAGCATCAG CCTGAAGCTG 
GACAGCGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874075.1
CDS	287..4021
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHEGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	XM_017018586.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018586.1

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGAAGGCTT CTCGGACAGT 
GAGGAGGTCT TCCCCAAGGA GATGACCAAG TGGAACTCCA ATGACTTCAT TGATGCCTTT 
GCCTCCCCAG TGGAGGCAGA GGGAACGCCA GAGCCTGGAG CCGAGGCAGC TAAAGGCATT 
GAGGATGGGG CCCAAGCACC CAGGGACTCA GAGGGCCTGG ATGGAGCCGG GGAGCTGGGG 
GCTGAGGCAT GCGCAGTCCA CGCCCTCTTC CTTATCCTGC ACAGCGGCAA CATCCTGGAC 
TCAGGCCCTG GAGACGCCAA CTCCAAGCAG GCGGATGTGC AGACGCTGAG CTCCGCCTTC 
GAGGCCGTCA CCCGCATCCA CTTCCCTGAG GCCTTGGGCC ACGTGGCGCT GCGACTGGTG 
CCCTGTCCAC CCATCTGCGC CGCCGCCTAT GCCCTTGTCT CCAACCTGAG CCCTTACAGC 
CACGATGGGG ACAGCCTGTC TCGCTCCCAA GACCACATTC CACTGGCTGC CCTGCCACTG 
CTGGCCACCT CATCCTCCCG CTACCAGGGC GCCGTGGCCA CCGTCATTGC CCGCACCAAC 
CAGGCCTACT CAGCCTTCCT GCGCTCACCT GAGGGTGCCG GCTTCTGTGG GCAGGTCGCA 
CTGATTGGAG ATGGTGTTGG TGGCATCCTG GGCTTTGATG CACTCTGCCA CAGTGCTAAC 
GCGGGCACCG GGAGTCGGGG CAGCAGCCGC CGTGGGAGCA TGAACAATGA GCTGCTCTCT 
CCGGAGTTTG GCCCAGTGCG GGACCCCCTG GCAGATGGTG TGGAAGGCCT GGGTCGGGGC 
AGCCCAGAAC CCTCGGCCTT GCCTCCCCAG CGCATCCCCA GCGACATGGC CAGTCCTGAG 
CCCGAGGGCT CTCAGAACAG CCTTCAGGCA GCCCCCGCAA CCACCTCCTC CTGGGAGCCC 
CGGCGGGCAA GCACGGCCTT CTGCCCACCC GCTGCCAGTT CCGAGGCACC TGACGGCCCC 
AGCAGCACTG CCCGCCTTGA CTTCAAGGTC TCTGGCTTCT TCCTCTTCGG CTCCCCACTG 
GGCCTGGTGC TGGCTCTGCG CAAAACTGTG ATGCCCGCCC TGGAGGCAGC CCAGATGCGC 
CCAGCCTGTG AACAGATCTA CAACCTCTTC CACGCGGCTG ACCCCTGCGC CTCACGCCTC 
GAGCCCCTGC TGGCCCCGAA GTTCCAGGCC ATCGCCCCAC TGACCGTGCC CCGCTACCAG 
AAGTTCCCCC TGGGAGATGG CTCATCCCTG CTGCTGGCCG ACACTCTGCA GACGCACTCC 
AGCCTCTTTC TGGAGGAGCT GGAGATGCTG GTGCCCTCAA CACCCACCTC TACTAGCGGT 
GCCTTCTGGA AGGGCAGTGA GTTGGCCACT GACCCCCCGG CCCAGCCAGC CGCCCCCAGC 
ACCACCAGTG AGGTGGTTAA GATCCTGGAG CGCTGGTGGG GGACCAAGCG GATCGACTAC 
TCGCTGTACT GCCCCGAGGC GCTCACCGCC TTTCCCACCG TCACGCTGCC CCACCTCTTC 
CACGCCAGCT ACTGGGAGTC CGCCGACGTG GTGGCGTTCA TCCTGCGCCA GGTGATCGAG 
AAGGAGCGGC CACAGCTGGC GGAATGCGAG GAGCCGTCCA TCTACAGCCC GGCCTTCCCC 
AGGGAGAAGT GGCAGCGAAA ACGCACGCAG GTCAAGATCC GGAACGTCAC TTCCAACCAC 
CGGGCGAGCG ACACGGTGGT GTGCGAGGGC CGCCCCCAGG TGCTAAGCGG GCGCTTCATG 
TACGGGCCCC TGGACGTCGT CACGCTCACT GGAGAGAAGG TGGATGTCTA CATCATGACG 
CAGCCGCTGT CGGGCAAGTG GATCCACTTT GGCACCGAAG TCACCAATAG CTCGGGCCGC 
CTCACCTTCC CAGTTCCCCC AGAACGCGCG CTGGGCATTG GTGTCTACCC CGTGCGCATG 
GTGGTCAGGG GCGACCACAC CTATGCCGAA TGCTGCCTGA CTGTGGTGGC CCGCGGCACG 
GAGGCTGTGG TCTTCAGCAT CGACGGCTCC TTCACCGCCA GCGTCTCCAT CATGGGCAGC 
GACCCCAAGG TGCGAGCTGG CGCCGTGGAC GTGGTCAGGC ACTGGCAGGA CTCCGGCTAC 
CTGATCGTGT ATGTCACAGG CCGGCCGGAT ATGCAGAAGC ACCGCGTGGT GGCATGGCTG 
TCGCAGCACA ACTTCCCCCA CGGCGTCGTC TCCTTCTGCG ACGGCCTCAC CCACGACCCA 
CTACGCCAGA AGGCAATGTT TCTGCAGAGC CTGGTGCAGG AGGTAGAACT GAACATCGTG 
GCCGGTTATG GGTCTCCCAA AGATGTGGCT GTATACGCGG CGCTGGGGCT GTCCCCGAGC 
CAGACCTACA TCGTGGGCCG TGCCGTGCGG AAGCTACAGG CGCAGTGCCA GTTCCTGTCA 
GACGGCTATG TGGCCCACCT GGGCCAGCTG GAAGCGGGCT CGCACTCGCA TGCCTCCTCG 
GGACCCCCGA GAGCTGCCTT GGGCAAGAGC AGCTATGGTG TGGCTGCCCC CGTGGACTTC 
CTGCGCAAAC AGAGCCAGCT GCTTCGCTCG AGGGGCCCCA GCCAGGCGGA GCGTGAGGGC 
CCGGGAACAC CACCCACCAC CCTGGCACGG GGCAAAGCAC GGAGCATCAG CCTGAAGCTG 
GACAGCGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITPNM1 ( XM_011545396.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543698.1 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X2, mRNA.

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CloneID	OHu62963
Clone ID Related Accession (Same CDS sequence)	XM_011545396.2 , XM_011545396.3
Accession Version	XM_011545396.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545396.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGGCTTCTC GGACAGTGAG 
GAGGTCTTCC CCAAGGAGAT GACCAAGTGG AACTCCAATG ACTTCATTGA TGCCTTTGCC 
TCCCCAGTGG AGGCAGAGGG AACGCCAGAG CCTGGAGCCG AGGCAGCTAA AGGCATTGAG 
GATGGGGCCC AAGCACCCAG GGACTCAGAG GGCCTGGATG GAGCCGGGGA GCTGGGGGCT 
GAGGCATGCG CAGTCCACGC CCTCTTCCTT ATCCTGCACA GCGGCAACAT CCTGGACTCA 
GGCCCTGGAG ACGCCAACTC CAAGCAGGCG GATGTGCAGA CGCTGAGCTC CGCCTTCGAG 
GCCGTCACCC GCATCCACTT CCCTGAGGCC TTGGGCCACG TGGCGCTGCG ACTGGTGCCC 
TGTCCACCCA TCTGCGCCGC CGCCTATGCC CTTGTCTCCA ACCTGAGCCC TTACAGCCAC 
GATGGGGACA GCCTGTCTCG CTCCCAAGAC CACATTCCAC TGGCTGCCCT GCCACTGCTG 
GCCACCTCAT CCTCCCGCTA CCAGGGCGCC GTGGCCACCG TCATTGCCCG CACCAACCAG 
GCCTACTCAG CCTTCCTGCG CTCACCTGAG GGTGCCGGCT TCTGTGGGCA GGTCGCACTG 
ATTGGAGATG GTGTTGGTGG CATCCTGGGC TTTGATGCAC TCTGCCACAG TGCTAACGCG 
GGCACCGGGA GTCGGGGCAG CAGCCGCCGT GGGAGCATGA ACAATGAGCT GCTCTCTCCG 
GAGTTTGGCC CAGTGCGGGA CCCCCTGGCA GATGGTGTGG AAGGCCTGGG TCGGGGCAGC 
CCAGAACCCT CGGCCTTGCC TCCCCAGCGC ATCCCCAGCG ACATGGCCAG TCCTGAGCCC 
GAGGGCTCTC AGAACAGCCT TCAGGCAGCC CCCGCAACCA CCTCCTCCTG GGAGCCCCGG 
CGGGCAAGCA CGGCCTTCTG CCCACCCGCT GCCAGTTCCG AGGCACCTGA CGGCCCCAGC 
AGCACTGCCC GCCTTGACTT CAAGGTCTCT GGCTTCTTCC TCTTCGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTGCGCAA AACTGTGATG CCCGCCCTGG AGGCAGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543698.1
CDS	246..3977
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	XM_011545396.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545396.2

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGGCTTCTC GGACAGTGAG 
GAGGTCTTCC CCAAGGAGAT GACCAAGTGG AACTCCAATG ACTTCATTGA TGCCTTTGCC 
TCCCCAGTGG AGGCAGAGGG AACGCCAGAG CCTGGAGCCG AGGCAGCTAA AGGCATTGAG 
GATGGGGCCC AAGCACCCAG GGACTCAGAG GGCCTGGATG GAGCCGGGGA GCTGGGGGCT 
GAGGCATGCG CAGTCCACGC CCTCTTCCTT ATCCTGCACA GCGGCAACAT CCTGGACTCA 
GGCCCTGGAG ACGCCAACTC CAAGCAGGCG GATGTGCAGA CGCTGAGCTC CGCCTTCGAG 
GCCGTCACCC GCATCCACTT CCCTGAGGCC TTGGGCCACG TGGCGCTGCG ACTGGTGCCC 
TGTCCACCCA TCTGCGCCGC CGCCTATGCC CTTGTCTCCA ACCTGAGCCC TTACAGCCAC 
GATGGGGACA GCCTGTCTCG CTCCCAAGAC CACATTCCAC TGGCTGCCCT GCCACTGCTG 
GCCACCTCAT CCTCCCGCTA CCAGGGCGCC GTGGCCACCG TCATTGCCCG CACCAACCAG 
GCCTACTCAG CCTTCCTGCG CTCACCTGAG GGTGCCGGCT TCTGTGGGCA GGTCGCACTG 
ATTGGAGATG GTGTTGGTGG CATCCTGGGC TTTGATGCAC TCTGCCACAG TGCTAACGCG 
GGCACCGGGA GTCGGGGCAG CAGCCGCCGT GGGAGCATGA ACAATGAGCT GCTCTCTCCG 
GAGTTTGGCC CAGTGCGGGA CCCCCTGGCA GATGGTGTGG AAGGCCTGGG TCGGGGCAGC 
CCAGAACCCT CGGCCTTGCC TCCCCAGCGC ATCCCCAGCG ACATGGCCAG TCCTGAGCCC 
GAGGGCTCTC AGAACAGCCT TCAGGCAGCC CCCGCAACCA CCTCCTCCTG GGAGCCCCGG 
CGGGCAAGCA CGGCCTTCTG CCCACCCGCT GCCAGTTCCG AGGCACCTGA CGGCCCCAGC 
AGCACTGCCC GCCTTGACTT CAAGGTCTCT GGCTTCTTCC TCTTCGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTGCGCAA AACTGTGATG CCCGCCCTGG AGGCAGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PITPNM1 ( XM_011545396.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543698.1 Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X2, mRNA.

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CloneID	OHu62963
Clone ID Related Accession (Same CDS sequence)	XM_011545396.2 , XM_011545396.3
Accession Version	XM_011545396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	membrane-associated phosphatidylinositol transfer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGGCTTCTC GGACAGTGAG 
GAGGTCTTCC CCAAGGAGAT GACCAAGTGG AACTCCAATG ACTTCATTGA TGCCTTTGCC 
TCCCCAGTGG AGGCAGAGGG AACGCCAGAG CCTGGAGCCG AGGCAGCTAA AGGCATTGAG 
GATGGGGCCC AAGCACCCAG GGACTCAGAG GGCCTGGATG GAGCCGGGGA GCTGGGGGCT 
GAGGCATGCG CAGTCCACGC CCTCTTCCTT ATCCTGCACA GCGGCAACAT CCTGGACTCA 
GGCCCTGGAG ACGCCAACTC CAAGCAGGCG GATGTGCAGA CGCTGAGCTC CGCCTTCGAG 
GCCGTCACCC GCATCCACTT CCCTGAGGCC TTGGGCCACG TGGCGCTGCG ACTGGTGCCC 
TGTCCACCCA TCTGCGCCGC CGCCTATGCC CTTGTCTCCA ACCTGAGCCC TTACAGCCAC 
GATGGGGACA GCCTGTCTCG CTCCCAAGAC CACATTCCAC TGGCTGCCCT GCCACTGCTG 
GCCACCTCAT CCTCCCGCTA CCAGGGCGCC GTGGCCACCG TCATTGCCCG CACCAACCAG 
GCCTACTCAG CCTTCCTGCG CTCACCTGAG GGTGCCGGCT TCTGTGGGCA GGTCGCACTG 
ATTGGAGATG GTGTTGGTGG CATCCTGGGC TTTGATGCAC TCTGCCACAG TGCTAACGCG 
GGCACCGGGA GTCGGGGCAG CAGCCGCCGT GGGAGCATGA ACAATGAGCT GCTCTCTCCG 
GAGTTTGGCC CAGTGCGGGA CCCCCTGGCA GATGGTGTGG AAGGCCTGGG TCGGGGCAGC 
CCAGAACCCT CGGCCTTGCC TCCCCAGCGC ATCCCCAGCG ACATGGCCAG TCCTGAGCCC 
GAGGGCTCTC AGAACAGCCT TCAGGCAGCC CCCGCAACCA CCTCCTCCTG GGAGCCCCGG 
CGGGCAAGCA CGGCCTTCTG CCCACCCGCT GCCAGTTCCG AGGCACCTGA CGGCCCCAGC 
AGCACTGCCC GCCTTGACTT CAAGGTCTCT GGCTTCTTCC TCTTCGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTGCGCAA AACTGTGATG CCCGCCCTGG AGGCAGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543698.1
CDS	227..3958
Translation	MLIKEYHILLPMSLDEYQVAQLYMIQKKSREESSGEGSGVEILANRPYTDGPGGSGQYTHKVYHVGSHIPGWFRALLPKAALQVEEESWNAYPYTRTRYTCPFVEKFSIEIETYYLPDGGQQPNVFNLSGAERRQRILDTIDIVRDAVAPGEYKAEEDPRLYHSVKTGRGPLSDDWARTAAQTGPLMCAYKLCKVEFRYWGMQAKIEQFIHDVGLRRVMLRAHRQAWCWQDEWTELSMADIRALEEETARMLAQRMAKCNTGSEGSEAQPPGKPSTEARSAASNTGTPDGPEAPPGPDASPDASFGKQWSSSSRSSYSSQHGGAVSPQSLSEWRMQNIARDSENSSEEEFFDAHGFSDSEEVFPKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGPGDANSKQADVQTLSSAFEAVTRIHFPEALGHVALRLVPCPPICAAAYALVSNLSPYSHDGDSLSRSQDHIPLAALPLLATSSSRYQGAVATVIARTNQAYSAFLRSPEGAGFCGQVALIGDGVGGILGFDALCHSANAGTGSRGSSRRGSMNNELLSPEFGPVRDPLADGVEGLGRGSPEPSALPPQRIPSDMASPEPEGSQNSLQAAPATTSSWEPRRASTAFCPPAASSEAPDGPSSTARLDFKVSGFFLFGSPLGLVLALRKTVMPALEAAQMRPACEQIYNLFHAADPCASRLEPLLAPKFQAIAPLTVPRYQKFPLGDGSSLLLADTLQTHSSLFLEELEMLVPSTPTSTSGAFWKGSELATDPPAQPAAPSTTSEVVKILERWWGTKRIDYSLYCPEALTAFPTVTLPHLFHASYWESADVVAFILRQVIEKERPQLAECEEPSIYSPAFPREKWQRKRTQVKIRNVTSNHRASDTVVCEGRPQVLSGRFMYGPLDVVTLTGEKVDVYIMTQPLSGKWIHFGTEVTNSSGRLTFPVPPERALGIGVYPVRMVVRGDHTYAECCLTVVARGTEAVVFSIDGSFTASVSIMGSDPKVRAGAVDVVRHWQDSGYLIVYVTGRPDMQKHRVVAWLSQHNFPHGVVSFCDGLTHDPLRQKAMFLQSLVQEVELNIVAGYGSPKDVAVYAALGLSPSQTYIVGRAVRKLQAQCQFLSDGYVAHLGQLEAGSHSHASSGPPRAALGKSSYGVAAPVDFLRKQSQLLRSRGPSQAEREGPGTPPTTLARGKARSISLKLDSEE

Target ORF information:

RefSeq Version	XM_011545396.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol transfer protein membrane associated 1 (PITPNM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545396.3

ORF Insert Sequence:

ATGCTCATCA AGGAATACCA CATTCTGCTG CCCATGAGCC TGGACGAGTA CCAGGTGGCC 
CAGCTCTACA TGATCCAGAA AAAGAGCCGG GAGGAGTCTA GTGGTGAGGG CAGCGGCGTG 
GAGATCCTGG CCAACCGGCC CTACACGGAT GGGCCCGGGG GCAGCGGGCA ATACACACAC 
AAGGTGTACC ACGTGGGCTC CCACATCCCA GGCTGGTTCC GGGCACTGCT GCCCAAGGCT 
GCCCTGCAGG TAGAAGAGGA ATCCTGGAAT GCCTACCCCT ACACCCGAAC CCGGTACACC 
TGCCCTTTCG TGGAGAAATT CTCCATTGAA ATTGAGACCT ATTACCTGCC TGATGGGGGG 
CAGCAGCCAA ACGTCTTCAA CCTGAGCGGG GCCGAGAGGA GACAGCGCAT CCTGGACACC 
ATCGACATCG TGCGGGATGC AGTGGCCCCA GGCGAGTACA AAGCAGAAGA GGACCCCCGG 
CTTTATCACT CGGTCAAGAC GGGCCGAGGG CCACTGTCTG ATGACTGGGC ACGGACGGCG 
GCACAGACGG GGCCCCTTAT GTGTGCCTAT AAGCTGTGCA AGGTTGAGTT CCGCTACTGG 
GGCATGCAAG CCAAGATCGA GCAGTTCATC CATGATGTAG GTCTGCGTCG GGTGATGCTG 
CGGGCCCACC GCCAGGCCTG GTGCTGGCAG GATGAGTGGA CAGAGCTGAG CATGGCTGAC 
ATCCGGGCAC TGGAAGAGGA GACTGCTCGC ATGCTGGCCC AGCGCATGGC CAAGTGCAAC 
ACAGGCAGTG AGGGGTCCGA GGCCCAGCCC CCCGGGAAAC CGAGCACCGA GGCCCGGTCT 
GCGGCCAGCA ACACTGGCAC CCCCGATGGG CCTGAGGCCC CCCCAGGCCC AGATGCCTCC 
CCCGATGCCA GCTTTGGGAA GCAGTGGTCC TCATCCTCCC GTTCCTCCTA CTCATCCCAA 
CATGGAGGGG CTGTGTCTCC CCAGAGCTTG TCTGAGTGGC GCATGCAGAA CATTGCCCGA 
GACTCTGAGA ACAGCTCCGA GGAAGAGTTC TTTGATGCCC ACGGCTTCTC GGACAGTGAG 
GAGGTCTTCC CCAAGGAGAT GACCAAGTGG AACTCCAATG ACTTCATTGA TGCCTTTGCC 
TCCCCAGTGG AGGCAGAGGG AACGCCAGAG CCTGGAGCCG AGGCAGCTAA AGGCATTGAG 
GATGGGGCCC AAGCACCCAG GGACTCAGAG GGCCTGGATG GAGCCGGGGA GCTGGGGGCT 
GAGGCATGCG CAGTCCACGC CCTCTTCCTT ATCCTGCACA GCGGCAACAT CCTGGACTCA 
GGCCCTGGAG ACGCCAACTC CAAGCAGGCG GATGTGCAGA CGCTGAGCTC CGCCTTCGAG 
GCCGTCACCC GCATCCACTT CCCTGAGGCC TTGGGCCACG TGGCGCTGCG ACTGGTGCCC 
TGTCCACCCA TCTGCGCCGC CGCCTATGCC CTTGTCTCCA ACCTGAGCCC TTACAGCCAC 
GATGGGGACA GCCTGTCTCG CTCCCAAGAC CACATTCCAC TGGCTGCCCT GCCACTGCTG 
GCCACCTCAT CCTCCCGCTA CCAGGGCGCC GTGGCCACCG TCATTGCCCG CACCAACCAG 
GCCTACTCAG CCTTCCTGCG CTCACCTGAG GGTGCCGGCT TCTGTGGGCA GGTCGCACTG 
ATTGGAGATG GTGTTGGTGG CATCCTGGGC TTTGATGCAC TCTGCCACAG TGCTAACGCG 
GGCACCGGGA GTCGGGGCAG CAGCCGCCGT GGGAGCATGA ACAATGAGCT GCTCTCTCCG 
GAGTTTGGCC CAGTGCGGGA CCCCCTGGCA GATGGTGTGG AAGGCCTGGG TCGGGGCAGC 
CCAGAACCCT CGGCCTTGCC TCCCCAGCGC ATCCCCAGCG ACATGGCCAG TCCTGAGCCC 
GAGGGCTCTC AGAACAGCCT TCAGGCAGCC CCCGCAACCA CCTCCTCCTG GGAGCCCCGG 
CGGGCAAGCA CGGCCTTCTG CCCACCCGCT GCCAGTTCCG AGGCACCTGA CGGCCCCAGC 
AGCACTGCCC GCCTTGACTT CAAGGTCTCT GGCTTCTTCC TCTTCGGCTC CCCACTGGGC 
CTGGTGCTGG CTCTGCGCAA AACTGTGATG CCCGCCCTGG AGGCAGCCCA GATGCGCCCA 
GCCTGTGAAC AGATCTACAA CCTCTTCCAC GCGGCTGACC CCTGCGCCTC ACGCCTCGAG 
CCCCTGCTGG CCCCGAAGTT CCAGGCCATC GCCCCACTGA CCGTGCCCCG CTACCAGAAG 
TTCCCCCTGG GAGATGGCTC ATCCCTGCTG CTGGCCGACA CTCTGCAGAC GCACTCCAGC 
CTCTTTCTGG AGGAGCTGGA GATGCTGGTG CCCTCAACAC CCACCTCTAC TAGCGGTGCC 
TTCTGGAAGG GCAGTGAGTT GGCCACTGAC CCCCCGGCCC AGCCAGCCGC CCCCAGCACC 
ACCAGTGAGG TGGTTAAGAT CCTGGAGCGC TGGTGGGGGA CCAAGCGGAT CGACTACTCG 
CTGTACTGCC CCGAGGCGCT CACCGCCTTT CCCACCGTCA CGCTGCCCCA CCTCTTCCAC 
GCCAGCTACT GGGAGTCCGC CGACGTGGTG GCGTTCATCC TGCGCCAGGT GATCGAGAAG 
GAGCGGCCAC AGCTGGCGGA ATGCGAGGAG CCGTCCATCT ACAGCCCGGC CTTCCCCAGG 
GAGAAGTGGC AGCGAAAACG CACGCAGGTC AAGATCCGGA ACGTCACTTC CAACCACCGG 
GCGAGCGACA CGGTGGTGTG CGAGGGCCGC CCCCAGGTGC TAAGCGGGCG CTTCATGTAC 
GGGCCCCTGG ACGTCGTCAC GCTCACTGGA GAGAAGGTGG ATGTCTACAT CATGACGCAG 
CCGCTGTCGG GCAAGTGGAT CCACTTTGGC ACCGAAGTCA CCAATAGCTC GGGCCGCCTC 
ACCTTCCCAG TTCCCCCAGA ACGCGCGCTG GGCATTGGTG TCTACCCCGT GCGCATGGTG 
GTCAGGGGCG ACCACACCTA TGCCGAATGC TGCCTGACTG TGGTGGCCCG CGGCACGGAG 
GCTGTGGTCT TCAGCATCGA CGGCTCCTTC ACCGCCAGCG TCTCCATCAT GGGCAGCGAC 
CCCAAGGTGC GAGCTGGCGC CGTGGACGTG GTCAGGCACT GGCAGGACTC CGGCTACCTG 
ATCGTGTATG TCACAGGCCG GCCGGATATG CAGAAGCACC GCGTGGTGGC ATGGCTGTCG 
CAGCACAACT TCCCCCACGG CGTCGTCTCC TTCTGCGACG GCCTCACCCA CGACCCACTA 
CGCCAGAAGG CAATGTTTCT GCAGAGCCTG GTGCAGGAGG TAGAACTGAA CATCGTGGCC 
GGTTATGGGT CTCCCAAAGA TGTGGCTGTA TACGCGGCGC TGGGGCTGTC CCCGAGCCAG 
ACCTACATCG TGGGCCGTGC CGTGCGGAAG CTACAGGCGC AGTGCCAGTT CCTGTCAGAC 
GGCTATGTGG CCCACCTGGG CCAGCTGGAA GCGGGCTCGC ACTCGCATGC CTCCTCGGGA 
CCCCCGAGAG CTGCCTTGGG CAAGAGCAGC TATGGTGTGG CTGCCCCCGT GGACTTCCTG 
CGCAAACAGA GCCAGCTGCT TCGCTCGAGG GGCCCCAGCC AGGCGGAGCG TGAGGGCCCG 
GGAACACCAC CCACCACCCT GGCACGGGGC AAAGCACGGA GCATCAGCCT GAAGCTGGAC 
AGCGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphatidylinositol 4,5-Bisphosphate Homeostasis Regulated by Nir2 and Nir3 Proteins at Endoplasmic Reticulum-Plasma Membrane Junctions.
The Journal of biological chemistry290(23)14289-301(2015 Jun)
Chang CL,Liou J

The lipid-transfer protein Nir2 enhances epithelial-mesenchymal transition and facilitates breast cancer metastasis.
Journal of cell science127(Pt 21)4740-9(2014 Nov)
Keinan O,Kedan A,Gavert N,Selitrennik M,Kim S,Karn T,Becker S,Lev S

Feedback regulation of receptor-induced Ca2+ signaling mediated by E-Syt1 and Nir2 at endoplasmic reticulum-plasma membrane junctions.
Cell reports5(3)813-25(2013 Nov)
Chang CL,Hsieh TS,Yang TT,Rothberg KG,Azizoglu DB,Volk E,Liao JC,Liou J

The phosphatidylinositol-transfer protein Nir2 binds phosphatidic acid and positively regulates phosphoinositide signalling.
EMBO reports14(10)891-9(2013 Oct)
Kim S,Kedan A,Marom M,Gavert N,Keinan O,Selitrennik M,Laufman O,Lev S

Phosphatidylinositol transfer protein, cytoplasmic 1 (PITPNC1) binds and transfers phosphatidic acid.
The Journal of biological chemistry287(38)32263-76(2012 Sep)
Garner K,Hunt AN,Koster G,Somerharju P,Groves E,Li M,Raghu P,Holic R,Cockcroft S

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