• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

SPATA2 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol SPATA2
Entrez Gene ID 9825
Full Name spermatogenesis associated 2
Synonyms PD1,PPP1R145,tamo
General protein information
Preferred Names

spermatogenesis associated 2

Names

spermatogenesis-associated protein 2
protein phosphatase 1, regulatory subunit 145
spermatogenesis associated PD1
spermatogenesis-associated protein PD1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

20

20q13.13
Disorder MIM:

607662


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001135773.1 NP_001129245.1 spermatogenesis-associated protein 2
XM_011529116.1 XP_011527418.1 spermatogenesis-associated protein 2 isoform X1
XM_006723894.1 XP_006723957.1 spermatogenesis-associated protein 2 isoform X1
NM_006038.3 NP_006029.1 spermatogenesis-associated protein 2
NM_006038.4 NP_006029.1 spermatogenesis-associated protein 2

Pathways from BioSystems


Homologies


Homo sapiens (human) SPATA2 NP_006029.1
Rattus norvegicus (Norway rat) Spata2 NP_446127.3
Danio rerio (zebrafish) spata2 NP_001073660.2
Xenopus tropicalis (tropical clawed frog) spata2 XP_002933200.1
Pan troglodytes (chimpanzee) SPATA2 XP_001167299.1
Mus musculus (house mouse) Spata2 NP_739562.1
Gallus gallus (chicken) SPATA2 NP_001007874.1
SPATA2 XP_001098177.1
Canis lupus familiaris (dog) SPATA2 XP_543047.2
Bos taurus (cattle) SPATA2 NP_001179609.1

Gene Ontology


Bibliography

Related articles in PubMed

SPATA2 promotes CYLD activity and regulates TNF-induced NF-?B signaling and cell death.
Schlicher L, Wissler M, Preiss F, Brauns-Schubert P, Jakob C, Dumit V, Borner C, Dengjel J, Maurer U
EMBO reports17(10)1485-1497(2016 10)

SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
Elliott PR, Leske D, Hrdinka M, Bagola K, Fiil BK, McLaughlin SH, Wagstaff J, Volkmar N, Christianson JC, Kessler BM, Freund SM, Komander D, Gyrd-Hansen M
Molecular cell63(6)990-1005(2016 Sep)

SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes.
Wagner SA, Satpathy S, Beli P, Choudhary C
The EMBO journal35(17)1868-84(2016 09)

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
Kupka S, De Miguel D, Draber P, Martino L, Surinova S, Rittinger K, Walczak H
Cell reports16(9)2271-80(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study identifies SPATA2 as an important factor in the TNF signaling pathway with a substantial role for the effects mediated by the cytokine.
Title: SPATA2 promotes CYLD activity and regulates TNF-induced NF-?B signaling and cell death.

The data reveal SPATA2 as a high-affinity binding partner of CYLD and HOIP, and a regulatory component of linear ubiquitin chain assembly complex-mediated NF-kappaB signaling.
Title: SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.

The predicted PUB domain in the N-terminus of SPATA2 interacts with the USP domain of CYLD and SPATA2 is required for recruitment of CYLD to the TNF-alpha receptor-associated signaling complexes.
Title: SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes.

SPATA2 has been described as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes.
Title: SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.

The aim of this study was to investigate whether the HCP5, TNIP1, TNFAIP3, SPATA2 and COG6 genes were genetic risk factors for psoriasis in Chinese population.
Title: Investigating the genetic association of HCP5, SPATA2, TNIP1, TNFAIP3 and COG6 with psoriasis in Chinese population.

The following SPATA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPATA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu28204 NM_001135773.1
Latest version!		 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu28204 NM_006038.4
Latest version!		 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu28204 XM_006723894.1
Latest version!		 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
OHu28204 XM_011529116.1
Latest version!		 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
View Old Accession Versions >>
OHu28204 NM_006038.3 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $219.50-$307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu28204
Clone ID Related Accession (Same CDS sequence) NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version NM_001135773.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-23
Organism Homo sapiens(human)
Product spermatogenesis-associated protein 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK289644.1, BC009481.2, AL031685.18 and N62318.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289644.1, SRR1803616.9152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001129245.1
CDS351..1913
Translation

Target ORF information:

RefSeq Version NM_001135773.1
Organism Homo sapiens(human)
Definition Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001135773.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28204
Clone ID Related Accession (Same CDS sequence) NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version NM_006038.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-11-19
Organism Homo sapiens(human)
Product spermatogenesis-associated protein 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295536.1, BC009481.2, AL031685.18 and N62318.1. On Oct 11, 2008 this sequence version replaced NM_006038.2. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018300.1, SRR1660807.54080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006029.1
CDS267..1829
Translation

Target ORF information:

RefSeq Version NM_006038.3
Organism Homo sapiens(human)
Definition Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006038.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28204
Clone ID Related Accession (Same CDS sequence) NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version NM_006038.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-27
Organism Homo sapiens(human)
Product spermatogenesis-associated protein 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295536.1, BC009481.2, AL031685.18 and N62318.1. On Nov 23, 2018 this sequence version replaced NM_006038.3. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.445800.1, AB018300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000289431.10/ ENSP00000289431.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006029.1
CDS253..1815
Translation

Target ORF information:

RefSeq Version NM_006038.4
Organism Homo sapiens(human)
Definition Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_006038.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28204
Clone ID Related Accession (Same CDS sequence) NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version XM_011529116.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product spermatogenesis-associated protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011527418.1
CDS224..1786
Translation

Target ORF information:

RefSeq Version XM_011529116.1
Organism Homo sapiens(human)
Definition Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011529116.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28204
Clone ID Related Accession (Same CDS sequence) NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version XM_006723894.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product spermatogenesis-associated protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006723957.1
CDS187..1749
Translation

Target ORF information:

RefSeq Version XM_006723894.1
Organism Homo sapiens(human)
Definition Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006723894.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

SPATA2 promotes CYLD activity and regulates TNF-induced NF-?B signaling and cell death.
EMBO reports17(10)1485-1497(2016 10)
Schlicher L,Wissler M,Preiss F,Brauns-Schubert P,Jakob C,Dumit V,Borner C,Dengjel J,Maurer U


book

SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
Molecular cell63(6)990-1005(2016 Sep)
Elliott PR,Leske D,Hrdinka M,Bagola K,Fiil BK,McLaughlin SH,Wagstaff J,Volkmar N,Christianson JC,Kessler BM,Freund SM,Komander D,Gyrd-Hansen M


book

SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes.
The EMBO journal35(17)1868-84(2016 09)
Wagner SA,Satpathy S,Beli P,Choudhary C


book

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
Cell reports16(9)2271-80(2016 08)
Kupka S,De Miguel D,Draber P,Martino L,Surinova S,Rittinger K,Walczak H