TRIM66 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006718397.2	XP_006718460.1	tripartite motif-containing protein 66 isoform X1
XM_011520515.1	XP_011518817.1	tripartite motif-containing protein 66 isoform X7
XM_011520518.1	XP_011518820.1	tripartite motif-containing protein 66 isoform X7
XM_011520523.2	XP_011518825.1	tripartite motif-containing protein 66 isoform X7
XM_011520526.2	XP_011518828.1	tripartite motif-containing protein 66 isoform X10
NM_014818.1	NP_055633.1	tripartite motif-containing protein 66
XM_011520507.2	XP_011518809.1	tripartite motif-containing protein 66 isoform X1
XM_011520508.1	XP_011518810.1	tripartite motif-containing protein 66 isoform X2
XM_017018629.1	XP_016874118.1	tripartite motif-containing protein 66 isoform X1
XM_011520507.3	XP_011518809.1	tripartite motif-containing protein 66 isoform X1
XM_011520508.2	XP_011518810.1	tripartite motif-containing protein 66 isoform X2
XM_017018630.1	XP_016874119.1	tripartite motif-containing protein 66 isoform X6
XM_011520512.1	XP_011518814.1	tripartite motif-containing protein 66 isoform X6
XM_011520514.2	XP_011518816.1	tripartite motif-containing protein 66 isoform X6
XM_011520517.2	XP_011518819.1	tripartite motif-containing protein 66 isoform X7
XM_011520519.1	XP_011518821.1	tripartite motif-containing protein 66 isoform X7
XM_011520527.1	XP_011518829.1	tripartite motif-containing protein 66 isoform X11
XM_011520504.1	XP_011518806.1	tripartite motif-containing protein 66 isoform X1
XM_011520509.1	XP_011518811.1	tripartite motif-containing protein 66 isoform X3
XM_011520513.1	XP_011518815.1	tripartite motif-containing protein 66 isoform X6
XM_011520522.2	XP_011518824.1	tripartite motif-containing protein 66 isoform X7
XM_011520511.1	XP_011518813.1	tripartite motif-containing protein 66 isoform X5
XM_011520516.1	XP_011518818.1	tripartite motif-containing protein 66 isoform X7
XM_006718398.3	XP_006718461.1	tripartite motif-containing protein 66 isoform X7
XM_011520524.1	XP_011518826.1	tripartite motif-containing protein 66 isoform X8
XM_011520510.1	XP_011518812.1	tripartite motif-containing protein 66 isoform X4
XM_017018631.1	XP_016874120.1	tripartite motif-containing protein 66 isoform X7
XM_011520525.1	XP_011518827.1	tripartite motif-containing protein 66 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu63427	XM_011520527.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu45122	XM_011520507.3 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu45122	XM_011520504.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu45122	XM_017018629.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu45122	XM_006718397.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu45123	XM_011520515.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_006718398.3 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_011520516.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_011520517.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_011520519.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_011520518.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_011520523.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_017018631.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu45123	XM_011520522.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu63419	XM_011520508.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu63420	XM_011520509.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu63421	XM_011520510.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu63422	XM_011520511.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu63423	XM_017018630.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu63423	XM_011520512.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu63423	XM_011520514.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu63423	XM_011520513.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu63424	XM_011520524.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu63425	XM_011520525.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu63426	XM_011520526.2 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu17490	NM_014818.1 Latest version!	Homo sapiens tripartite motif containing 66 (TRIM66), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$860.30 $1229.00
View Old Accession Versions >>
OHu45122	XM_011520507.2	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
OHu63419	XM_011520508.1	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$727.30 $1039.00
Hide Old Accession Versions >>

TRIM66 ( XM_011520527.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518829.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu63427
Clone ID Related Accession (Same CDS sequence)	XM_011520527.1
Accession Version	XM_011520527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATAA TGCATCACAA GTTTGAGCTG GAGGAAATGC AGAAGGACTT GGAGCTTCTT 
CTCCAGGCTC AACAGCCCAG CCTGCAACTG AGTCAGACCA AATCTCCTCA GCATCTTCAG 
CAAACCATTG TGGGGCAGAT CAACTACATC GTGAGGCAGC CAGCACCTGT CCAGTCCCAG 
AGCCAGGAGG AGACCCTGCA GGCTACAGAT GAGCCCCCAG CATCTCAGGG CTCAAAGCCG 
GCTCTCCCTC TTGACAAGAA TACTGCTGCT GCCTTGCCCC AGGCGTCTGG GGAAGAAACC 
CCTCTCAGTG TCCCCCCAGT GGACAGCACC ATCCAGCACT CCTCTCCAAA TGTGGTGAGA 
AAGCACTCCA CCTCGCTGAG CATCATGGGC TTTTCCAACA CTCTGGAGAT GGAGTTGTCA 
TCTACCAGGT TGGAGAGGCC CCTAGAGCCA CAGATCCAGA GTGTGAGCAA CCTGACAGCT 
GGTGCCCCCC AGGCAGTACC AAGCCTGCTG AGTGCTCCCC CCAAAATGGT GTCCAGCCTG 
ACAAGTGTTC AAAACCAGGC CATGCCCAGC CTGACAACCA GTCACCTACA GACTGTGCCC 
AGCCTTGTGC ATAGCACATT CCAGTCCATG CCCAACCTGA TAAGTGACTC CCCTCAGGCT 
ATGGCAAGCC TGGCAAGTGA TCACCCTCAG GCTGGGCCCA GCCTAATGTC TGGTCACACC 
CAGGCTGTGC CGAGTCTGGC AACTTGTCCT CTGCAGAGCA TCCCTCCAGT TTCTGACATG 
CAGCCAGAAA CTGGGTCCAG CTCCAGTTCT GGCCGAACTT CAGGGAGCCT GTGTCCCAGA 
GATGGGGCTG ATCCCTCCCT GGAGAATGCT CTGTGTAAGA TGGAAAGTGA GGATTCCACT 
CGCTTCACTG ACTTACTGGG ACAAGGTCCC ATAGTCCCCG GTCTGGATGC TCCCAAGGAC 
TTGGCCATCC CCTCAGAACT GGAGGAGCCA ATTAACCTCT CTGTGAAGAA ACCTCCACTG 
GCGCCAGTGG TCAGCACGTC TACAGCTCTG CAGCAGTACC AGAACCCAAA AGAGTGTGAG 
AATTTTGAAC AAGGAGCCCT AGAGCTGGAT GCAAAAGAGA ACCAGAGCAT CAGAGCCTTC 
AATAGTGAGC ATAAGATTCC CTATGTGCGA CTGGAGCGAC TCAAGATCTG TGCTGCCTCC 
TCAGGAGAGA TGCCTGTGTT CAAACTGAAG CCACAGAAGA ATGATCAGGA TGGGAGCTTC 
CTGCTGATCA TCGAGTGTGG CACTGAGTCC TCCAGCATGT CCATTAAGGT CAGCCAGGAC 
AGACTGTCTG AGGCCACCCA GGCCCCAGGT CTGGAGGGAA GAAAGGTCAC TGTCACTTCT 
TTGGCTGGGC AGCGGCCACC AGAAGTGGAG GGCACATCTC CTGAAGAACA CAGACTCATT 
CCTCGAACCC CAGGAGCCAA GAAGGGCCCC CCAGCCCCAA TAGAGAATGA GGACTTCTGT 
GCTGTTTGCC TCAATGGCGG AGAGTTACTG TGCTGTGACC GCTGCCCCAA AGTGTTCCAC 
CTCTCCTGCC ATGTGCCAGC CTTGCTCAGC TTCCCAGGGG GAGAGTGGGT GTGTACCTTG 
TGCCGCAGCC TGACCCAGCC CGAGATGGAG TACGACTGTG AGAATGCCTG CTATAACCAG 
CCTGGAATGC GGGCATCTCC TGGCCTAAGC ATGTATGACC AGAAGAAGTG TGAGAAGCTG 
GTATTGTCCT TGTGCTGCAA TAACCTCAGC CTGCCCTTCC ATGAACCTGT CAGCCCCCTG 
GCCCGGCATT ATTACCAGAT TATCAAGAGG CCCATGGACC TGTCAATCAT CCGGAGGAAG 
CTGCAAAAGA AGGACCCAGC TCACTATACC ACCCCAGAGG AGGTGGTATC AGATGTGCGC 
CTCATGTTCT GGAACTGTGC TAAGTTCAAT TATCCTGACT CCGAGGTTGC AGAGGCTGGC 
CGCTGCCTGG AAGTGTTCTT TGAGGGCTGG TTGAAGGAGA TCTACCCGGA GAAACGGTTT 
GCCCAGCCAA GGCAGGAGGA CTCAGACTCC GAGGAGGTGT CTAGTGAGAG TGGATGTTCC 
ACTCCCCAGG GCTTCCCGTG GCCTCCCTAC ATGCAGGAGG GCATCCAACC CAAGAGGCGG 
CGACGACATA TGGAGAATGA AAGAGCAAAA AGAATGTCAT TTCGCCTGGC CAACAGCATC 
TCTCAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518829.1
CDS	139..2370
Translation	MDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520527.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520527.1

ORF Insert Sequence:

ATGGACATAA TGCATCACAA GTTTGAGCTG GAGGAAATGC AGAAGGACTT GGAGCTTCTT 
CTCCAGGCTC AACAGCCCAG CCTGCAACTG AGTCAGACCA AATCTCCTCA GCATCTTCAG 
CAAACCATTG TGGGGCAGAT CAACTACATC GTGAGGCAGC CAGCACCTGT CCAGTCCCAG 
AGCCAGGAGG AGACCCTGCA GGCTACAGAT GAGCCCCCAG CATCTCAGGG CTCAAAGCCG 
GCTCTCCCTC TTGACAAGAA TACTGCTGCT GCCTTGCCCC AGGCGTCTGG GGAAGAAACC 
CCTCTCAGTG TCCCCCCAGT GGACAGCACC ATCCAGCACT CCTCTCCAAA TGTGGTGAGA 
AAGCACTCCA CCTCGCTGAG CATCATGGGC TTTTCCAACA CTCTGGAGAT GGAGTTGTCA 
TCTACCAGGT TGGAGAGGCC CCTAGAGCCA CAGATCCAGA GTGTGAGCAA CCTGACAGCT 
GGTGCCCCCC AGGCAGTACC AAGCCTGCTG AGTGCTCCCC CCAAAATGGT GTCCAGCCTG 
ACAAGTGTTC AAAACCAGGC CATGCCCAGC CTGACAACCA GTCACCTACA GACTGTGCCC 
AGCCTTGTGC ATAGCACATT CCAGTCCATG CCCAACCTGA TAAGTGACTC CCCTCAGGCT 
ATGGCAAGCC TGGCAAGTGA TCACCCTCAG GCTGGGCCCA GCCTAATGTC TGGTCACACC 
CAGGCTGTGC CGAGTCTGGC AACTTGTCCT CTGCAGAGCA TCCCTCCAGT TTCTGACATG 
CAGCCAGAAA CTGGGTCCAG CTCCAGTTCT GGCCGAACTT CAGGGAGCCT GTGTCCCAGA 
GATGGGGCTG ATCCCTCCCT GGAGAATGCT CTGTGTAAGA TGGAAAGTGA GGATTCCACT 
CGCTTCACTG ACTTACTGGG ACAAGGTCCC ATAGTCCCCG GTCTGGATGC TCCCAAGGAC 
TTGGCCATCC CCTCAGAACT GGAGGAGCCA ATTAACCTCT CTGTGAAGAA ACCTCCACTG 
GCGCCAGTGG TCAGCACGTC TACAGCTCTG CAGCAGTACC AGAACCCAAA AGAGTGTGAG 
AATTTTGAAC AAGGAGCCCT AGAGCTGGAT GCAAAAGAGA ACCAGAGCAT CAGAGCCTTC 
AATAGTGAGC ATAAGATTCC CTATGTGCGA CTGGAGCGAC TCAAGATCTG TGCTGCCTCC 
TCAGGAGAGA TGCCTGTGTT CAAACTGAAG CCACAGAAGA ATGATCAGGA TGGGAGCTTC 
CTGCTGATCA TCGAGTGTGG CACTGAGTCC TCCAGCATGT CCATTAAGGT CAGCCAGGAC 
AGACTGTCTG AGGCCACCCA GGCCCCAGGT CTGGAGGGAA GAAAGGTCAC TGTCACTTCT 
TTGGCTGGGC AGCGGCCACC AGAAGTGGAG GGCACATCTC CTGAAGAACA CAGACTCATT 
CCTCGAACCC CAGGAGCCAA GAAGGGCCCC CCAGCCCCAA TAGAGAATGA GGACTTCTGT 
GCTGTTTGCC TCAATGGCGG AGAGTTACTG TGCTGTGACC GCTGCCCCAA AGTGTTCCAC 
CTCTCCTGCC ATGTGCCAGC CTTGCTCAGC TTCCCAGGGG GAGAGTGGGT GTGTACCTTG 
TGCCGCAGCC TGACCCAGCC CGAGATGGAG TACGACTGTG AGAATGCCTG CTATAACCAG 
CCTGGAATGC GGGCATCTCC TGGCCTAAGC ATGTATGACC AGAAGAAGTG TGAGAAGCTG 
GTATTGTCCT TGTGCTGCAA TAACCTCAGC CTGCCCTTCC ATGAACCTGT CAGCCCCCTG 
GCCCGGCATT ATTACCAGAT TATCAAGAGG CCCATGGACC TGTCAATCAT CCGGAGGAAG 
CTGCAAAAGA AGGACCCAGC TCACTATACC ACCCCAGAGG AGGTGGTATC AGATGTGCGC 
CTCATGTTCT GGAACTGTGC TAAGTTCAAT TATCCTGACT CCGAGGTTGC AGAGGCTGGC 
CGCTGCCTGG AAGTGTTCTT TGAGGGCTGG TTGAAGGAGA TCTACCCGGA GAAACGGTTT 
GCCCAGCCAA GGCAGGAGGA CTCAGACTCC GAGGAGGTGT CTAGTGAGAG TGGATGTTCC 
ACTCCCCAGG GCTTCCCGTG GCCTCCCTAC ATGCAGGAGG GCATCCAACC CAAGAGGCGG 
CGACGACATA TGGAGAATGA AAGAGCAAAA AGAATGTCAT TTCGCCTGGC CAACAGCATC 
TCTCAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_006718397.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718460.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu45122
Clone ID Related Accession (Same CDS sequence)	XM_006718397.2 , XM_011520507.2 , XM_017018629.1 , XM_011520507.3 , XM_011520504.1
Accession Version	XM_006718397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006718397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718460.1
CDS	606..4784
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_006718397.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718397.2

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520507.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518809.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

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CloneID	OHu45122
Clone ID Related Accession (Same CDS sequence)	XM_006718397.2 , XM_011520507.2 , XM_017018629.1 , XM_011520507.3 , XM_011520504.1
Accession Version	XM_011520507.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518809.1
CDS	570..4748
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520507.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520507.2

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_017018629.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874118.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu45122
Clone ID Related Accession (Same CDS sequence)	XM_006718397.2 , XM_011520507.2 , XM_017018629.1 , XM_011520507.3 , XM_011520504.1
Accession Version	XM_017018629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874118.1
CDS	999..5177
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_017018629.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018629.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520507.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518809.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu45122
Clone ID Related Accession (Same CDS sequence)	XM_006718397.2 , XM_011520507.2 , XM_017018629.1 , XM_011520507.3 , XM_011520504.1
Accession Version	XM_011520507.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518809.1
CDS	627..4805
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520507.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520507.3

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520504.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518806.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

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CloneID	OHu45122
Clone ID Related Accession (Same CDS sequence)	XM_006718397.2 , XM_011520507.2 , XM_017018629.1 , XM_011520507.3 , XM_011520504.1
Accession Version	XM_011520504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518806.1
CDS	649..4827
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520504.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520504.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG AGAATGAAAG AGCAAAAAGA 
ATGTCATTTC GCCTGGCCAA CAGCATCTCT CAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520515.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518817.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X13, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518817.1
CDS	472..4215
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520515.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520515.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520518.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518820.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518820.1
CDS	442..4185
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520518.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520523.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518825.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518825.1
CDS	2390..6133
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520523.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520523.2

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520517.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518819.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X15, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518819.1
CDS	1794..5537
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520517.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520517.2

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520519.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518821.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X18, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518821.1
CDS	330..4073
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520519.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520519.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520522.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518824.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X21, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518824.1
CDS	1508..5251
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520522.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520522.2

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520516.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518818.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X14, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_011520516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518818.1
CDS	472..4215
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520516.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520516.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_006718398.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718461.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X16, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_006718398.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718461.1
CDS	1653..5396
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_006718398.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718398.3

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_017018631.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874120.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X20, mRNA.

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CloneID	OHu45123
Clone ID Related Accession (Same CDS sequence)	XM_011520515.1 , XM_011520518.1 , XM_011520523.2 , XM_011520517.2 , XM_011520519.1 , XM_011520522.2 , XM_011520516.1 , XM_006718398.3 , XM_017018631.1
Accession Version	XM_017018631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874120.1
CDS	548..4291
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_017018631.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018631.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GATGGAAAGT 
GAGGATTCCA CTCGCTTCAC TGACTTACTG GGACAAGGTC CCATAGTCCC CGGTCTGGAT 
GCTCCCAAGG ACTTGGCCAT CCCCTCAGAA CTGGAGGAGC CAATTAACCT CTCTGTGAAG 
AAACCTCCAC TGGCGCCAGT GGTCAGCACG TCTACAGCTC TGCAGCAGTA CCAGAACCCA 
AAAGAGTGTG AGAATTTTGA ACAAGGAGCC CTAGAGCTGG ATGCAAAAGA GAACCAGAGC 
ATCAGAGCCT TCAATAGTGA GCATAAGATT CCCTATGTGC GACTGGAGCG ACTCAAGATC 
TGTGCTGCCT CCTCAGGAGA GATGCCTGTG TTCAAACTGA AGCCACAGAA GAATGATCAG 
GATGGGAGCT TCCTGCTGAT CATCGAGTGT GGCACTGAGT CCTCCAGCAT GTCCATTAAG 
GTCAGCCAGG ACAGACTGTC TGAGGCCACC CAGGCCCCAG GTCTGGAGGG AAGAAAGGTC 
ACTGTCACTT CTTTGGCTGG GCAGCGGCCA CCAGAAGTGG AGGGCACATC TCCTGAAGAA 
CACAGACTCA TTCCTCGAAC CCCAGGAGCC AAGAAGGGCC CCCCAGCCCC AATAGAGAAT 
GAGGACTTCT GTGCTGTTTG CCTCAATGGC GGAGAGTTAC TGTGCTGTGA CCGCTGCCCC 
AAAGTGTTCC ACCTCTCCTG CCATGTGCCA GCCTTGCTCA GCTTCCCAGG GGGAGAGTGG 
GTGTGTACCT TGTGCCGCAG CCTGACCCAG CCCGAGATGG AGTACGACTG TGAGAATGCC 
TGCTATAACC AGCCTGGAAT GCGGGCATCT CCTGGCCTAA GCATGTATGA CCAGAAGAAG 
TGTGAGAAGC TGGTATTGTC CTTGTGCTGC AATAACCTCA GCCTGCCCTT CCATGAACCT 
GTCAGCCCCC TGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520508.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518810.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

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CloneID	OHu63419
Clone ID Related Accession (Same CDS sequence)	XM_011520508.1 , XM_011520508.2
Accession Version	XM_011520508.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG TAAAGAGTTA CTGCCAGCCA 
GCAGCAGGTG GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518810.1
CDS	606..4763
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMVKSYCQPAAGGR

Target ORF information:

RefSeq Version	XM_011520508.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520508.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG TAAAGAGTTA CTGCCAGCCA 
GCAGCAGGTG GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520508.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518810.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

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CloneID	OHu63419
Clone ID Related Accession (Same CDS sequence)	XM_011520508.1 , XM_011520508.2
Accession Version	XM_011520508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG TAAAGAGTTA CTGCCAGCCA 
GCAGCAGGTG GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518810.1
CDS	606..4763
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMVKSYCQPAAGGR

Target ORF information:

RefSeq Version	XM_011520508.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520508.2

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGAAGTGTGA GAAGCTGGTA TTGTCCTTGT GCTGCAATAA CCTCAGCCTG 
CCCTTCCATG AACCTGTCAG CCCCCTGGCC CGGCATTATT ACCAGATTAT CAAGAGGCCC 
ATGGACCTGT CAATCATCCG GAGGAAGCTG CAAAAGAAGG ACCCAGCTCA CTATACCACC 
CCAGAGGAGG TGGTATCAGA TGTGCGCCTC ATGTTCTGGA ACTGTGCTAA GTTCAATTAT 
CCTGACTCCG AGGTTGCAGA GGCTGGCCGC TGCCTGGAAG TGTTCTTTGA GGGCTGGTTG 
AAGGAGATCT ACCCGGAGAA ACGGTTTGCC CAGCCAAGGC AGGAGGACTC AGACTCCGAG 
GAGGTGTCTA GTGAGAGTGG ATGTTCCACT CCCCAGGGCT TCCCGTGGCC TCCCTACATG 
CAGGAGGGCA TCCAACCCAA GAGGCGGCGA CGACATATGG TAAAGAGTTA CTGCCAGCCA 
GCAGCAGGTG GGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520509.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518811.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu63420
Clone ID Related Accession (Same CDS sequence)	XM_011520509.1
Accession Version	XM_011520509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518811.1
CDS	606..4709
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520509.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520509.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGGTCAG CCAGGACAGA CTGTCTGAGG CCACCCAGGC CCCAGGTCTG 
GAGGGAAGAA AGGTCACTGT CACTTCTTTG GCTGGGCAGC GGCCACCAGA AGTGGAGGGC 
ACATCTCCTG AAGAACACAG ACTCATTCCT CGAACCCCAG GAGCCAAGAA GGGCCCCCCA 
GCCCCAATAG AGAATGAGGA CTTCTGTGCT GTTTGCCTCA ATGGCGGAGA GTTACTGTGC 
TGTGACCGCT GCCCCAAAGT GTTCCACCTC TCCTGCCATG TGCCAGCCTT GCTCAGCTTC 
CCAGGGGGAG AGTGGGTGTG TACCTTGTGC CGCAGCCTGA CCCAGCCCGA GATGGAGTAC 
GACTGTGAGA ATGCCTGCTA TAACCAGCCT GGAATGCGGG CATCTCCTGG CCTAAGCATG 
TATGACCAGA AGGCCCGGCA TTATTACCAG ATTATCAAGA GGCCCATGGA CCTGTCAATC 
ATCCGGAGGA AGCTGCAAAA GAAGGACCCA GCTCACTATA CCACCCCAGA GGAGGTGGTA 
TCAGATGTGC GCCTCATGTT CTGGAACTGT GCTAAGTTCA ATTATCCTGA CTCCGAGGTT 
GCAGAGGCTG GCCGCTGCCT GGAAGTGTTC TTTGAGGGCT GGTTGAAGGA GATCTACCCG 
GAGAAACGGT TTGCCCAGCC AAGGCAGGAG GACTCAGACT CCGAGGAGGT GTCTAGTGAG 
AGTGGATGTT CCACTCCCCA GGGCTTCCCG TGGCCTCCCT ACATGCAGGA GGGCATCCAA 
CCCAAGAGGC GGCGACGACA TATGGAGAAT GAAAGAGCAA AAAGAATGTC ATTTCGCCTG 
GCCAACAGCA TCTCTCAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520510.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518812.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

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CloneID	OHu63421
Clone ID Related Accession (Same CDS sequence)	XM_011520510.1
Accession Version	XM_011520510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGCGGCC ACCAGAAGTG GAGGGCACAT CTCCTGAAGA ACACAGACTC 
ATTCCTCGAA CCCCAGGAGC CAAGAAGGGC CCCCCAGCCC CAATAGAGAA TGAGGACTTC 
TGTGCTGTTT GCCTCAATGG CGGAGAGTTA CTGTGCTGTG ACCGCTGCCC CAAAGTGTTC 
CACCTCTCCT GCCATGTGCC AGCCTTGCTC AGCTTCCCAG GGGGAGAGTG GGTGTGTACC 
TTGTGCCGCA GCCTGACCCA GCCCGAGATG GAGTACGACT GTGAGAATGC CTGCTATAAC 
CAGCCTGGAA TGCGGGCATC TCCTGGCCTA AGCATGTATG ACCAGAAGAA GTGTGAGAAG 
CTGGTATTGT CCTTGTGCTG CAATAACCTC AGCCTGCCCT TCCATGAACC TGTCAGCCCC 
CTGGCCCGGC ATTATTACCA GATTATCAAG AGGCCCATGG ACCTGTCAAT CATCCGGAGG 
AAGCTGCAAA AGAAGGACCC AGCTCACTAT ACCACCCCAG AGGAGGTGGT ATCAGATGTG 
CGCCTCATGT TCTGGAACTG TGCTAAGTTC AATTATCCTG ACTCCGAGGT TGCAGAGGCT 
GGCCGCTGCC TGGAAGTGTT CTTTGAGGGC TGGTTGAAGG AGATCTACCC GGAGAAACGG 
TTTGCCCAGC CAAGGCAGGA GGACTCAGAC TCCGAGGAGG TGTCTAGTGA GAGTGGATGT 
TCCACTCCCC AGGGCTTCCC GTGGCCTCCC TACATGCAGG AGGGCATCCA ACCCAAGAGG 
CGGCGACGAC ATATGGAGAA TGAAAGAGCA AAAAGAATGT CATTTCGCCT GGCCAACAGC 
ATCTCTCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518812.1
CDS	606..4700
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520510.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520510.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG AGCCTTCAAT AGTGAGCATA AGATTCCCTA TGTGCGACTG 
GAGCGACTCA AGATCTGTGC TGCCTCCTCA GGAGAGATGC CTGTGTTCAA ACTGAAGCCA 
CAGAAGAATG ATCAGGATGG GAGCTTCCTG CTGATCATCG AGTGTGGCAC TGAGTCCTCC 
AGCATGTCCA TTAAGCGGCC ACCAGAAGTG GAGGGCACAT CTCCTGAAGA ACACAGACTC 
ATTCCTCGAA CCCCAGGAGC CAAGAAGGGC CCCCCAGCCC CAATAGAGAA TGAGGACTTC 
TGTGCTGTTT GCCTCAATGG CGGAGAGTTA CTGTGCTGTG ACCGCTGCCC CAAAGTGTTC 
CACCTCTCCT GCCATGTGCC AGCCTTGCTC AGCTTCCCAG GGGGAGAGTG GGTGTGTACC 
TTGTGCCGCA GCCTGACCCA GCCCGAGATG GAGTACGACT GTGAGAATGC CTGCTATAAC 
CAGCCTGGAA TGCGGGCATC TCCTGGCCTA AGCATGTATG ACCAGAAGAA GTGTGAGAAG 
CTGGTATTGT CCTTGTGCTG CAATAACCTC AGCCTGCCCT TCCATGAACC TGTCAGCCCC 
CTGGCCCGGC ATTATTACCA GATTATCAAG AGGCCCATGG ACCTGTCAAT CATCCGGAGG 
AAGCTGCAAA AGAAGGACCC AGCTCACTAT ACCACCCCAG AGGAGGTGGT ATCAGATGTG 
CGCCTCATGT TCTGGAACTG TGCTAAGTTC AATTATCCTG ACTCCGAGGT TGCAGAGGCT 
GGCCGCTGCC TGGAAGTGTT CTTTGAGGGC TGGTTGAAGG AGATCTACCC GGAGAAACGG 
TTTGCCCAGC CAAGGCAGGA GGACTCAGAC TCCGAGGAGG TGTCTAGTGA GAGTGGATGT 
TCCACTCCCC AGGGCTTCCC GTGGCCTCCC TACATGCAGG AGGGCATCCA ACCCAAGAGG 
CGGCGACGAC ATATGGAGAA TGAAAGAGCA AAAAGAATGT CATTTCGCCT GGCCAACAGC 
ATCTCTCAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520511.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518813.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

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CloneID	OHu63422
Clone ID Related Accession (Same CDS sequence)	XM_011520511.1
Accession Version	XM_011520511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGA GCTCATCTCC 
TGTCCTGGGT GTGAACGAGT ATATCTTACC AGGGATGTAA CTGAACATTT TTTTCTGCAT 
TGTGTTCCTA CTGAGCAACC CAAGATGGCC AGGAACTGCT CTGAGTGCAA GGAGAAGAGG 
GCAGCACATA TCCTCTGCAC CTACTGCAAT CGCTGGCTGT GCAGCTCTTG CACAGAGGAA 
CACCGACACA GCCCTGTCCC CGGGGGCCCA TTCTTTCCTC GGGCCCAGAA GGGATCTCCA 
GGAGTGAATG GTGGTCCCGG AGACTTCACC TTGTATTGTC CTCTACACAC ACAGGAAGTA 
CTCAAGCTAT TCTGTGAGAC ATGTGATATG CTCACTTGCC ATAGCTGCCT AGTGGTGGAA 
CACAAAGAAC ACAGGTGCAG ACATGTTGAA GAAGTTTTGC AAAACCAGAG GATGCTTCTG 
GAAGGTGTGA CTACACAGGT GGCACATAAG AAATCCAGTC TACAGACATC TGCAAAGCAA 
ATTGAGGACA GGATTTTTGA AGTGAAGCAT CAGCATAGGA AGGTGGAAAA CCAGATCAAA 
ATGGCCAAGA TGGTTCTGAT GAATGAGCTG AACAAACAGG CCAATGGGCT AATAGAGGAA 
TTAGAGGGGA TTACTAATGA GAGAAAGCGG AAGCTGGAAC AGCAGTTACA GAGCATCATG 
GTTCTCAACC GTCAGTTTGA GCATGTGCAG AATTTCATCA ACTGGGCTGT CTGCAGCAAA 
ACCAGTGTCC CTTTTCTTTT CAGCAAAGAG CTGATTGTGT TTCAGATGCA GCGATTGCTG 
GAGACAAGTT GTAACACAGA TCCTGGCTCC CCTTGGAGTA TCAGATTCAC CTGGGAGCCT 
AACTTCTGGA CCAAGCAGCT AGCTTCTCTT GGCTGCATAA CTACTGAAGG TGGACAAATG 
TCCAGGGCAG ATGCTCCTGC TTATGGAGGC TTACAGGGGT CATCACCCTT TTATCAAAGC 
CACCAGTCTC CAGTGGCTCA GCAAGAGGCT CTTAGCCACC CCTCACACAA GTTCCAGTCT 
CCAGCAGTGT GCTCCTCATC TGTGTGCTGC TCCCACTGCT CCCCAGTCTC GCCTTCCCTC 
AAAGGCCAGG TCCCCCCACC CAGCATACAC CCAGCCCACA GCTTCAGGCA GCCCCCTGAG 
ATGGTGCCCC AGCAGCTGGG GTCTCTGCAG TGCTCTGCCC TGCTGCCCAG GGAGAAAGAG 
CTGGCCTGCA GCCCTCATCC ACCAAAGCTG CTGCAGCCCT GGCTGGAAAC CCAGCCCCCC 
GTGGAGCAGG AGAGCACATC CCAGCGGCTG GGGCAGCAGC TGACTTCCCA GCCCGTGTGC 
ATTGTCCCCC CACAGGATGT TCAGCAAGGA GCCCATGCCC AGCCCACCTT ACAGACACCC 
TCTATCCAAG TCCAGTTTGG CCACCACCAG AAGCTGAAGC TCAGTCACTT TCAGCAGCAG 
CCACAGCAGC AGCTACCACC TCCACCACCA CCCCTCCCCC ATCCCCCACC TCCCCTTCCC 
CCTCCCCCAC AGCAGCCACA CCCACCTCTT CCTCCATCCC AGCATCTGGC TTCTAGTCAG 
CACGAGAGCC CTCCTGGCCC TGCCTGTTCT CAGAACATGG ACATAATGCA TCACAAGTTT 
GAGCTGGAGG AAATGCAGAA GGACTTGGAG CTTCTTCTCC AGGCTCAACA GCCCAGCCTG 
CAACTGAGTC AGACCAAATC TCCTCAGCAT CTTCAGCAAA CCATTGTGGG GCAGATCAAC 
TACATCGTGA GGCAGCCAGC ACCTGTCCAG TCCCAGAGCC AGGAGGAGAC CCTGCAGGCT 
ACAGATGAGC CCCCAGCATC TCAGGGCTCA AAGCCGGCTC TCCCTCTTGA CAAGAATACT 
GCTGCTGCCT TGCCCCAGGC GTCTGGGGAA GAAACCCCTC TCAGTGTCCC CCCAGTGGAC 
AGCACCATCC AGCACTCCTC TCCAAATGTG GTGAGAAAGC ACTCCACCTC GCTGAGCATC 
ATGGGCTTTT CCAACACTCT GGAGATGGAG TTGTCATCTA CCAGGTTGGA GAGGCCCCTA 
GAGCCACAGA TCCAGAGTGT GAGCAACCTG ACAGCTGGTG CCCCCCAGGC AGTACCAAGC 
CTGCTGAGTG CTCCCCCCAA AATGGTGTCC AGCCTGACAA GTGTTCAAAA CCAGGCCATG 
CCCAGCCTGA CAACCAGTCA CCTACAGACT GTGCCCAGCC TTGTGCATAG CACATTCCAG 
TCCATGCCCA ACCTGATAAG TGACTCCCCT CAGGCTATGG CAAGCCTGGC AAGTGATCAC 
CCTCAGGCTG GGCCCAGCCT AATGTCTGGT CACACCCAGG CTGTGCCGAG TCTGGCAACT 
TGTCCTCTGC AGAGCATCCC TCCAGTTTCT GACATGCAGC CAGAAACTGG GTCCAGCTCC 
AGTTCTGGCC GAACTTCAGG GAGCCTGTGT CCCAGAGATG GGGCTGATCC CTCCCTGGAG 
AATGCTCTGT GTAAGATGGA AAGTGAGGAT TCCACTCGCT TCACTGACTT ACTGGGACAA 
GGTCCCATAG TCCCCGGTCT GGATGCTCCC AAGGACTTGG CCATCCCCTC AGAACTGGAG 
GAGCCAATTA ACCTCTCTGT GAAGAAACCT CCACTGGCGC CAGTGGTCAG CACGTCTACA 
GCTCTGCAGC AGTACCAGAA CCCAAAAGAG TGTGAGAATT TTGAACAAGG AGCCCTAGAG 
CTGGATGCAA AAGAGAACCA GAGCATCAGA GCCTTCAATA GTGAGCATAA GATTCCCTAT 
GTGCGACTGG AGCGACTCAA GATCTGTGCT GCCTCCTCAG GAGAGATGCC TGTGTTCAAA 
CTGAAGCCAC AGAAGAATGA TCAGGATGGG AGCTTCCTGC TGATCATCGA GTGTGGCACT 
GAGTCCTCCA GCATGTCCAT TAAGGTCAGC CAGGACAGAC TGTCTGAGGC CACCCAGGCC 
CCAGGTCTGG AGGGAAGAAA GGTCACTGTC ACTTCTTTGG CTGGGCAGCG GCCACCAGAA 
GTGGAGGGCA CATCTCCTGA AGAACACAGA CTCATTCCTC GAACCCCAGG AGCCAAGAAG 
GGCCCCCCAG CCCCAATAGA GAATGAGGAC TTCTGTGCTG TTTGCCTCAA TGGCGGAGAG 
TTACTGTGCT GTGACCGCTG CCCCAAAGTG TTCCACCTCT CCTGCCATGT GCCAGCCTTG 
CTCAGCTTCC CAGGGGGAGA GTGGGTGTGT ACCTTGTGCC GCAGCCTGAC CCAGCCCGAG 
ATGGAGTACG ACTGTGAGAA TGCCTGCTAT AACCAGCCTG GAATGCGGGC ATCTCCTGGC 
CTAAGCATGT ATGACCAGAA GAAGTGTGAG AAGCTGGTAT TGTCCTTGTG CTGCAATAAC 
CTCAGCCTGC CCTTCCATGA ACCTGTCAGC CCCCTGGCCC GGCATTATTA CCAGATTATC 
AAGAGGCCCA TGGACCTGTC AATCATCCGG AGGAAGCTGC AAAAGAAGGA CCCAGCTCAC 
TATACCACCC CAGAGGAGGT GGTATCAGAT GTGCGCCTCA TGTTCTGGAA CTGTGCTAAG 
TTCAATTATC CTGACTCCGA GGTTGCAGAG GCTGGCCGCT GCCTGGAAGT GTTCTTTGAG 
GGCTGGTTGA AGGAGATCTA CCCGGAGAAA CGGTTTGCCC AGCCAAGGCA GGAGGACTCA 
GACTCCGAGG AGGTGTCTAG TGAGAGTGGA TGTTCCACTC CCCAGGGCTT CCCGTGGCCT 
CCCTACATGC AGGAGGGCAT CCAACCCAAG AGGCGGCGAC GACATATGGA GAATGAAAGA 
GCAAAAAGAA TGTCATTTCG CCTGGCCAAC AGCATCTCTC AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518813.1
CDS	606..4673
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520511.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520511.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGA GCTCATCTCC 
TGTCCTGGGT GTGAACGAGT ATATCTTACC AGGGATGTAA CTGAACATTT TTTTCTGCAT 
TGTGTTCCTA CTGAGCAACC CAAGATGGCC AGGAACTGCT CTGAGTGCAA GGAGAAGAGG 
GCAGCACATA TCCTCTGCAC CTACTGCAAT CGCTGGCTGT GCAGCTCTTG CACAGAGGAA 
CACCGACACA GCCCTGTCCC CGGGGGCCCA TTCTTTCCTC GGGCCCAGAA GGGATCTCCA 
GGAGTGAATG GTGGTCCCGG AGACTTCACC TTGTATTGTC CTCTACACAC ACAGGAAGTA 
CTCAAGCTAT TCTGTGAGAC ATGTGATATG CTCACTTGCC ATAGCTGCCT AGTGGTGGAA 
CACAAAGAAC ACAGGTGCAG ACATGTTGAA GAAGTTTTGC AAAACCAGAG GATGCTTCTG 
GAAGGTGTGA CTACACAGGT GGCACATAAG AAATCCAGTC TACAGACATC TGCAAAGCAA 
ATTGAGGACA GGATTTTTGA AGTGAAGCAT CAGCATAGGA AGGTGGAAAA CCAGATCAAA 
ATGGCCAAGA TGGTTCTGAT GAATGAGCTG AACAAACAGG CCAATGGGCT AATAGAGGAA 
TTAGAGGGGA TTACTAATGA GAGAAAGCGG AAGCTGGAAC AGCAGTTACA GAGCATCATG 
GTTCTCAACC GTCAGTTTGA GCATGTGCAG AATTTCATCA ACTGGGCTGT CTGCAGCAAA 
ACCAGTGTCC CTTTTCTTTT CAGCAAAGAG CTGATTGTGT TTCAGATGCA GCGATTGCTG 
GAGACAAGTT GTAACACAGA TCCTGGCTCC CCTTGGAGTA TCAGATTCAC CTGGGAGCCT 
AACTTCTGGA CCAAGCAGCT AGCTTCTCTT GGCTGCATAA CTACTGAAGG TGGACAAATG 
TCCAGGGCAG ATGCTCCTGC TTATGGAGGC TTACAGGGGT CATCACCCTT TTATCAAAGC 
CACCAGTCTC CAGTGGCTCA GCAAGAGGCT CTTAGCCACC CCTCACACAA GTTCCAGTCT 
CCAGCAGTGT GCTCCTCATC TGTGTGCTGC TCCCACTGCT CCCCAGTCTC GCCTTCCCTC 
AAAGGCCAGG TCCCCCCACC CAGCATACAC CCAGCCCACA GCTTCAGGCA GCCCCCTGAG 
ATGGTGCCCC AGCAGCTGGG GTCTCTGCAG TGCTCTGCCC TGCTGCCCAG GGAGAAAGAG 
CTGGCCTGCA GCCCTCATCC ACCAAAGCTG CTGCAGCCCT GGCTGGAAAC CCAGCCCCCC 
GTGGAGCAGG AGAGCACATC CCAGCGGCTG GGGCAGCAGC TGACTTCCCA GCCCGTGTGC 
ATTGTCCCCC CACAGGATGT TCAGCAAGGA GCCCATGCCC AGCCCACCTT ACAGACACCC 
TCTATCCAAG TCCAGTTTGG CCACCACCAG AAGCTGAAGC TCAGTCACTT TCAGCAGCAG 
CCACAGCAGC AGCTACCACC TCCACCACCA CCCCTCCCCC ATCCCCCACC TCCCCTTCCC 
CCTCCCCCAC AGCAGCCACA CCCACCTCTT CCTCCATCCC AGCATCTGGC TTCTAGTCAG 
CACGAGAGCC CTCCTGGCCC TGCCTGTTCT CAGAACATGG ACATAATGCA TCACAAGTTT 
GAGCTGGAGG AAATGCAGAA GGACTTGGAG CTTCTTCTCC AGGCTCAACA GCCCAGCCTG 
CAACTGAGTC AGACCAAATC TCCTCAGCAT CTTCAGCAAA CCATTGTGGG GCAGATCAAC 
TACATCGTGA GGCAGCCAGC ACCTGTCCAG TCCCAGAGCC AGGAGGAGAC CCTGCAGGCT 
ACAGATGAGC CCCCAGCATC TCAGGGCTCA AAGCCGGCTC TCCCTCTTGA CAAGAATACT 
GCTGCTGCCT TGCCCCAGGC GTCTGGGGAA GAAACCCCTC TCAGTGTCCC CCCAGTGGAC 
AGCACCATCC AGCACTCCTC TCCAAATGTG GTGAGAAAGC ACTCCACCTC GCTGAGCATC 
ATGGGCTTTT CCAACACTCT GGAGATGGAG TTGTCATCTA CCAGGTTGGA GAGGCCCCTA 
GAGCCACAGA TCCAGAGTGT GAGCAACCTG ACAGCTGGTG CCCCCCAGGC AGTACCAAGC 
CTGCTGAGTG CTCCCCCCAA AATGGTGTCC AGCCTGACAA GTGTTCAAAA CCAGGCCATG 
CCCAGCCTGA CAACCAGTCA CCTACAGACT GTGCCCAGCC TTGTGCATAG CACATTCCAG 
TCCATGCCCA ACCTGATAAG TGACTCCCCT CAGGCTATGG CAAGCCTGGC AAGTGATCAC 
CCTCAGGCTG GGCCCAGCCT AATGTCTGGT CACACCCAGG CTGTGCCGAG TCTGGCAACT 
TGTCCTCTGC AGAGCATCCC TCCAGTTTCT GACATGCAGC CAGAAACTGG GTCCAGCTCC 
AGTTCTGGCC GAACTTCAGG GAGCCTGTGT CCCAGAGATG GGGCTGATCC CTCCCTGGAG 
AATGCTCTGT GTAAGATGGA AAGTGAGGAT TCCACTCGCT TCACTGACTT ACTGGGACAA 
GGTCCCATAG TCCCCGGTCT GGATGCTCCC AAGGACTTGG CCATCCCCTC AGAACTGGAG 
GAGCCAATTA ACCTCTCTGT GAAGAAACCT CCACTGGCGC CAGTGGTCAG CACGTCTACA 
GCTCTGCAGC AGTACCAGAA CCCAAAAGAG TGTGAGAATT TTGAACAAGG AGCCCTAGAG 
CTGGATGCAA AAGAGAACCA GAGCATCAGA GCCTTCAATA GTGAGCATAA GATTCCCTAT 
GTGCGACTGG AGCGACTCAA GATCTGTGCT GCCTCCTCAG GAGAGATGCC TGTGTTCAAA 
CTGAAGCCAC AGAAGAATGA TCAGGATGGG AGCTTCCTGC TGATCATCGA GTGTGGCACT 
GAGTCCTCCA GCATGTCCAT TAAGGTCAGC CAGGACAGAC TGTCTGAGGC CACCCAGGCC 
CCAGGTCTGG AGGGAAGAAA GGTCACTGTC ACTTCTTTGG CTGGGCAGCG GCCACCAGAA 
GTGGAGGGCA CATCTCCTGA AGAACACAGA CTCATTCCTC GAACCCCAGG AGCCAAGAAG 
GGCCCCCCAG CCCCAATAGA GAATGAGGAC TTCTGTGCTG TTTGCCTCAA TGGCGGAGAG 
TTACTGTGCT GTGACCGCTG CCCCAAAGTG TTCCACCTCT CCTGCCATGT GCCAGCCTTG 
CTCAGCTTCC CAGGGGGAGA GTGGGTGTGT ACCTTGTGCC GCAGCCTGAC CCAGCCCGAG 
ATGGAGTACG ACTGTGAGAA TGCCTGCTAT AACCAGCCTG GAATGCGGGC ATCTCCTGGC 
CTAAGCATGT ATGACCAGAA GAAGTGTGAG AAGCTGGTAT TGTCCTTGTG CTGCAATAAC 
CTCAGCCTGC CCTTCCATGA ACCTGTCAGC CCCCTGGCCC GGCATTATTA CCAGATTATC 
AAGAGGCCCA TGGACCTGTC AATCATCCGG AGGAAGCTGC AAAAGAAGGA CCCAGCTCAC 
TATACCACCC CAGAGGAGGT GGTATCAGAT GTGCGCCTCA TGTTCTGGAA CTGTGCTAAG 
TTCAATTATC CTGACTCCGA GGTTGCAGAG GCTGGCCGCT GCCTGGAAGT GTTCTTTGAG 
GGCTGGTTGA AGGAGATCTA CCCGGAGAAA CGGTTTGCCC AGCCAAGGCA GGAGGACTCA 
GACTCCGAGG AGGTGTCTAG TGAGAGTGGA TGTTCCACTC CCCAGGGCTT CCCGTGGCCT 
CCCTACATGC AGGAGGGCAT CCAACCCAAG AGGCGGCGAC GACATATGGA GAATGAAAGA 
GCAAAAAGAA TGTCATTTCG CCTGGCCAAC AGCATCTCTC AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_017018630.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874119.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu63423
Clone ID Related Accession (Same CDS sequence)	XM_017018630.1 , XM_011520512.1 , XM_011520514.2 , XM_011520513.1
Accession Version	XM_017018630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874119.1
CDS	219..4139
Translation	MGLRHLSGTEETSWARSSKANDGDQSKELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_017018630.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018630.1

ORF Insert Sequence:

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520512.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518814.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X10, mRNA.

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CloneID	OHu63423
Clone ID Related Accession (Same CDS sequence)	XM_017018630.1 , XM_011520512.1 , XM_011520514.2 , XM_011520513.1
Accession Version	XM_011520512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518814.1
CDS	128..4048
Translation	MGLRHLSGTEETSWARSSKANDGDQSKELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520512.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520512.1

ORF Insert Sequence:

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520514.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518816.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu63423
Clone ID Related Accession (Same CDS sequence)	XM_017018630.1 , XM_011520512.1 , XM_011520514.2 , XM_011520513.1
Accession Version	XM_011520514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518816.1
CDS	82..4002
Translation	MGLRHLSGTEETSWARSSKANDGDQSKELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520514.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520514.2

ORF Insert Sequence:

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520513.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518815.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu63423
Clone ID Related Accession (Same CDS sequence)	XM_017018630.1 , XM_011520512.1 , XM_011520514.2 , XM_011520513.1
Accession Version	XM_011520513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518815.1
CDS	126..4046
Translation	MGLRHLSGTEETSWARSSKANDGDQSKELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520513.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520513.1

ORF Insert Sequence:

ATGGGTCTGC GTCACCTCTC AGGTACTGAG GAGACCAGCT GGGCAAGATC TTCAAAAGCT 
AATGATGGGG ACCAGAGCAA AGAGCTCATC TCCTGTCCTG GGTGTGAACG AGTATATCTT 
ACCAGGGATG TAACTGAACA TTTTTTTCTG CATTGTGTTC CTACTGAGCA ACCCAAGATG 
GCCAGGAACT GCTCTGAGTG CAAGGAGAAG AGGGCAGCAC ATATCCTCTG CACCTACTGC 
AATCGCTGGC TGTGCAGCTC TTGCACAGAG GAACACCGAC ACAGCCCTGT CCCCGGGGGC 
CCATTCTTTC CTCGGGCCCA GAAGGGATCT CCAGGAGTGA ATGGTGGTCC CGGAGACTTC 
ACCTTGTATT GTCCTCTACA CACACAGGAA GTACTCAAGC TATTCTGTGA GACATGTGAT 
ATGCTCACTT GCCATAGCTG CCTAGTGGTG GAACACAAAG AACACAGGTG CAGACATGTT 
GAAGAAGTTT TGCAAAACCA GAGGATGCTT CTGGAAGGTG TGACTACACA GGTGGCACAT 
AAGAAATCCA GTCTACAGAC ATCTGCAAAG CAAATTGAGG ACAGGATTTT TGAAGTGAAG 
CATCAGCATA GGAAGGTGGA AAACCAGATC AAAATGGCCA AGATGGTTCT GATGAATGAG 
CTGAACAAAC AGGCCAATGG GCTAATAGAG GAATTAGAGG GGATTACTAA TGAGAGAAAG 
CGGAAGCTGG AACAGCAGTT ACAGAGCATC ATGGTTCTCA ACCGTCAGTT TGAGCATGTG 
CAGAATTTCA TCAACTGGGC TGTCTGCAGC AAAACCAGTG TCCCTTTTCT TTTCAGCAAA 
GAGCTGATTG TGTTTCAGAT GCAGCGATTG CTGGAGACAA GTTGTAACAC AGATCCTGGC 
TCCCCTTGGA GTATCAGATT CACCTGGGAG CCTAACTTCT GGACCAAGCA GCTAGCTTCT 
CTTGGCTGCA TAACTACTGA AGGTGGACAA ATGTCCAGGG CAGATGCTCC TGCTTATGGA 
GGCTTACAGG GGTCATCACC CTTTTATCAA AGCCACCAGT CTCCAGTGGC TCAGCAAGAG 
GCTCTTAGCC ACCCCTCACA CAAGTTCCAG TCTCCAGCAG TGTGCTCCTC ATCTGTGTGC 
TGCTCCCACT GCTCCCCAGT CTCGCCTTCC CTCAAAGGCC AGGTCCCCCC ACCCAGCATA 
CACCCAGCCC ACAGCTTCAG GCAGCCCCCT GAGATGGTGC CCCAGCAGCT GGGGTCTCTG 
CAGTGCTCTG CCCTGCTGCC CAGGGAGAAA GAGCTGGCCT GCAGCCCTCA TCCACCAAAG 
CTGCTGCAGC CCTGGCTGGA AACCCAGCCC CCCGTGGAGC AGGAGAGCAC ATCCCAGCGG 
CTGGGGCAGC AGCTGACTTC CCAGCCCGTG TGCATTGTCC CCCCACAGGA TGTTCAGCAA 
GGAGCCCATG CCCAGCCCAC CTTACAGACA CCCTCTATCC AAGTCCAGTT TGGCCACCAC 
CAGAAGCTGA AGCTCAGTCA CTTTCAGCAG CAGCCACAGC AGCAGCTACC ACCTCCACCA 
CCACCCCTCC CCCATCCCCC ACCTCCCCTT CCCCCTCCCC CACAGCAGCC ACACCCACCT 
CTTCCTCCAT CCCAGCATCT GGCTTCTAGT CAGCACGAGA GCCCTCCTGG CCCTGCCTGT 
TCTCAGAACA TGGACATAAT GCATCACAAG TTTGAGCTGG AGGAAATGCA GAAGGACTTG 
GAGCTTCTTC TCCAGGCTCA ACAGCCCAGC CTGCAACTGA GTCAGACCAA ATCTCCTCAG 
CATCTTCAGC AAACCATTGT GGGGCAGATC AACTACATCG TGAGGCAGCC AGCACCTGTC 
CAGTCCCAGA GCCAGGAGGA GACCCTGCAG GCTACAGATG AGCCCCCAGC ATCTCAGGGC 
TCAAAGCCGG CTCTCCCTCT TGACAAGAAT ACTGCTGCTG CCTTGCCCCA GGCGTCTGGG 
GAAGAAACCC CTCTCAGTGT CCCCCCAGTG GACAGCACCA TCCAGCACTC CTCTCCAAAT 
GTGGTGAGAA AGCACTCCAC CTCGCTGAGC ATCATGGGCT TTTCCAACAC TCTGGAGATG 
GAGTTGTCAT CTACCAGGTT GGAGAGGCCC CTAGAGCCAC AGATCCAGAG TGTGAGCAAC 
CTGACAGCTG GTGCCCCCCA GGCAGTACCA AGCCTGCTGA GTGCTCCCCC CAAAATGGTG 
TCCAGCCTGA CAAGTGTTCA AAACCAGGCC ATGCCCAGCC TGACAACCAG TCACCTACAG 
ACTGTGCCCA GCCTTGTGCA TAGCACATTC CAGTCCATGC CCAACCTGAT AAGTGACTCC 
CCTCAGGCTA TGGCAAGCCT GGCAAGTGAT CACCCTCAGG CTGGGCCCAG CCTAATGTCT 
GGTCACACCC AGGCTGTGCC GAGTCTGGCA ACTTGTCCTC TGCAGAGCAT CCCTCCAGTT 
TCTGACATGC AGCCAGAAAC TGGGTCCAGC TCCAGTTCTG GCCGAACTTC AGGGAGCCTG 
TGTCCCAGAG ATGGGGCTGA TCCCTCCCTG GAGAATGCTC TGTGTAAGAT GGAAAGTGAG 
GATTCCACTC GCTTCACTGA CTTACTGGGA CAAGGTCCCA TAGTCCCCGG TCTGGATGCT 
CCCAAGGACT TGGCCATCCC CTCAGAACTG GAGGAGCCAA TTAACCTCTC TGTGAAGAAA 
CCTCCACTGG CGCCAGTGGT CAGCACGTCT ACAGCTCTGC AGCAGTACCA GAACCCAAAA 
GAGTGTGAGA ATTTTGAACA AGGAGCCCTA GAGCTGGATG CAAAAGAGAA CCAGAGCATC 
AGAGCCTTCA ATAGTGAGCA TAAGATTCCC TATGTGCGAC TGGAGCGACT CAAGATCTGT 
GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC AAACTGAAGC CACAGAAGAA TGATCAGGAT 
GGGAGCTTCC TGCTGATCAT CGAGTGTGGC ACTGAGTCCT CCAGCATGTC CATTAAGGTC 
AGCCAGGACA GACTGTCTGA GGCCACCCAG GCCCCAGGTC TGGAGGGAAG AAAGGTCACT 
GTCACTTCTT TGGCTGGGCA GCGGCCACCA GAAGTGGAGG GCACATCTCC TGAAGAACAC 
AGACTCATTC CTCGAACCCC AGGAGCCAAG AAGGGCCCCC CAGCCCCAAT AGAGAATGAG 
GACTTCTGTG CTGTTTGCCT CAATGGCGGA GAGTTACTGT GCTGTGACCG CTGCCCCAAA 
GTGTTCCACC TCTCCTGCCA TGTGCCAGCC TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG 
TGTACCTTGT GCCGCAGCCT GACCCAGCCC GAGATGGAGT ACGACTGTGA GAATGCCTGC 
TATAACCAGC CTGGAATGCG GGCATCTCCT GGCCTAAGCA TGTATGACCA GAAGAAGTGT 
GAGAAGCTGG TATTGTCCTT GTGCTGCAAT AACCTCAGCC TGCCCTTCCA TGAACCTGTC 
AGCCCCCTGG CCCGGCATTA TTACCAGATT ATCAAGAGGC CCATGGACCT GTCAATCATC 
CGGAGGAAGC TGCAAAAGAA GGACCCAGCT CACTATACCA CCCCAGAGGA GGTGGTATCA 
GATGTGCGCC TCATGTTCTG GAACTGTGCT AAGTTCAATT ATCCTGACTC CGAGGTTGCA 
GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT GAGGGCTGGT TGAAGGAGAT CTACCCGGAG 
AAACGGTTTG CCCAGCCAAG GCAGGAGGAC TCAGACTCCG AGGAGGTGTC TAGTGAGAGT 
GGATGTTCCA CTCCCCAGGG CTTCCCGTGG CCTCCCTACA TGCAGGAGGG CATCCAACCC 
AAGAGGCGGC GACGACATAT GGAGAATGAA AGAGCAAAAA GAATGTCATT TCGCCTGGCC 
AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520524.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518826.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X24, mRNA.

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CloneID	OHu63424
Clone ID Related Accession (Same CDS sequence)	XM_011520524.1
Accession Version	XM_011520524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG GGATCATGGA TGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518826.1
CDS	606..3704
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRDHGCF

Target ORF information:

RefSeq Version	XM_011520524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520524.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGA GTGTGAGAAT TTTGAACAAG GAGCCCTAGA GCTGGATGCA 
AAAGAGAACC AGAGCATCAG GGATCATGGA TGTTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520525.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518827.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu63425
Clone ID Related Accession (Same CDS sequence)	XM_011520525.1
Accession Version	XM_011520525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGG TGAGACTCAA GCAGCCTGCC CTTCATGTTG GCCACAGACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518827.1
CDS	606..3668
Translation	MARLSFWSQGVELARSTRCFSPEDISGKAPVLGTGMAVDMGMSFMGLPLAGQKHCPKSGQMEAMVMTCSLCHQDLPGMGSHLLSCQHLLRKDCFQGLIQELGQIAKAHETVADELISCPGCERVYLTRDVTEHFFLHCVPTEQPKMARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKGETQAACPSCWPQT

Target ORF information:

RefSeq Version	XM_011520525.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520525.1

ORF Insert Sequence:

ATGGCTAGAC TCTCTTTTTG GTCCCAGGGA GTGGAGCTGG CTCGCTCTAC ACGCTGCTTC 
TCACCTGAGG ATATCAGTGG AAAAGCCCCA GTCCTGGGCA CAGGCATGGC TGTGGACATG 
GGCATGAGCT TTATGGGGCT GCCACTAGCT GGCCAGAAAC ACTGCCCTAA GAGTGGACAG 
ATGGAGGCCA TGGTAATGAC CTGCTCATTG TGCCATCAGG ACCTGCCAGG TATGGGCTCT 
CATCTCCTAT CCTGCCAGCA TTTGCTCCGT AAGGACTGCT TCCAGGGCTT GATACAGGAG 
CTAGGGCAGA TTGCCAAGGC TCATGAGACT GTTGCAGATG AGCTCATCTC CTGTCCTGGG 
TGTGAACGAG TATATCTTAC CAGGGATGTA ACTGAACATT TTTTTCTGCA TTGTGTTCCT 
ACTGAGCAAC CCAAGATGGC CAGGAACTGC TCTGAGTGCA AGGAGAAGAG GGCAGCACAT 
ATCCTCTGCA CCTACTGCAA TCGCTGGCTG TGCAGCTCTT GCACAGAGGA ACACCGACAC 
AGCCCTGTCC CCGGGGGCCC ATTCTTTCCT CGGGCCCAGA AGGGATCTCC AGGAGTGAAT 
GGTGGTCCCG GAGACTTCAC CTTGTATTGT CCTCTACACA CACAGGAAGT ACTCAAGCTA 
TTCTGTGAGA CATGTGATAT GCTCACTTGC CATAGCTGCC TAGTGGTGGA ACACAAAGAA 
CACAGGTGCA GACATGTTGA AGAAGTTTTG CAAAACCAGA GGATGCTTCT GGAAGGTGTG 
ACTACACAGG TGGCACATAA GAAATCCAGT CTACAGACAT CTGCAAAGCA AATTGAGGAC 
AGGATTTTTG AAGTGAAGCA TCAGCATAGG AAGGTGGAAA ACCAGATCAA AATGGCCAAG 
ATGGTTCTGA TGAATGAGCT GAACAAACAG GCCAATGGGC TAATAGAGGA ATTAGAGGGG 
ATTACTAATG AGAGAAAGCG GAAGCTGGAA CAGCAGTTAC AGAGCATCAT GGTTCTCAAC 
CGTCAGTTTG AGCATGTGCA GAATTTCATC AACTGGGCTG TCTGCAGCAA AACCAGTGTC 
CCTTTTCTTT TCAGCAAAGA GCTGATTGTG TTTCAGATGC AGCGATTGCT GGAGACAAGT 
TGTAACACAG ATCCTGGCTC CCCTTGGAGT ATCAGATTCA CCTGGGAGCC TAACTTCTGG 
ACCAAGCAGC TAGCTTCTCT TGGCTGCATA ACTACTGAAG GTGGACAAAT GTCCAGGGCA 
GATGCTCCTG CTTATGGAGG CTTACAGGGG TCATCACCCT TTTATCAAAG CCACCAGTCT 
CCAGTGGCTC AGCAAGAGGC TCTTAGCCAC CCCTCACACA AGTTCCAGTC TCCAGCAGTG 
TGCTCCTCAT CTGTGTGCTG CTCCCACTGC TCCCCAGTCT CGCCTTCCCT CAAAGGCCAG 
GTCCCCCCAC CCAGCATACA CCCAGCCCAC AGCTTCAGGC AGCCCCCTGA GATGGTGCCC 
CAGCAGCTGG GGTCTCTGCA GTGCTCTGCC CTGCTGCCCA GGGAGAAAGA GCTGGCCTGC 
AGCCCTCATC CACCAAAGCT GCTGCAGCCC TGGCTGGAAA CCCAGCCCCC CGTGGAGCAG 
GAGAGCACAT CCCAGCGGCT GGGGCAGCAG CTGACTTCCC AGCCCGTGTG CATTGTCCCC 
CCACAGGATG TTCAGCAAGG AGCCCATGCC CAGCCCACCT TACAGACACC CTCTATCCAA 
GTCCAGTTTG GCCACCACCA GAAGCTGAAG CTCAGTCACT TTCAGCAGCA GCCACAGCAG 
CAGCTACCAC CTCCACCACC ACCCCTCCCC CATCCCCCAC CTCCCCTTCC CCCTCCCCCA 
CAGCAGCCAC ACCCACCTCT TCCTCCATCC CAGCATCTGG CTTCTAGTCA GCACGAGAGC 
CCTCCTGGCC CTGCCTGTTC TCAGAACATG GACATAATGC ATCACAAGTT TGAGCTGGAG 
GAAATGCAGA AGGACTTGGA GCTTCTTCTC CAGGCTCAAC AGCCCAGCCT GCAACTGAGT 
CAGACCAAAT CTCCTCAGCA TCTTCAGCAA ACCATTGTGG GGCAGATCAA CTACATCGTG 
AGGCAGCCAG CACCTGTCCA GTCCCAGAGC CAGGAGGAGA CCCTGCAGGC TACAGATGAG 
CCCCCAGCAT CTCAGGGCTC AAAGCCGGCT CTCCCTCTTG ACAAGAATAC TGCTGCTGCC 
TTGCCCCAGG CGTCTGGGGA AGAAACCCCT CTCAGTGTCC CCCCAGTGGA CAGCACCATC 
CAGCACTCCT CTCCAAATGT GGTGAGAAAG CACTCCACCT CGCTGAGCAT CATGGGCTTT 
TCCAACACTC TGGAGATGGA GTTGTCATCT ACCAGGTTGG AGAGGCCCCT AGAGCCACAG 
ATCCAGAGTG TGAGCAACCT GACAGCTGGT GCCCCCCAGG CAGTACCAAG CCTGCTGAGT 
GCTCCCCCCA AAATGGTGTC CAGCCTGACA AGTGTTCAAA ACCAGGCCAT GCCCAGCCTG 
ACAACCAGTC ACCTACAGAC TGTGCCCAGC CTTGTGCATA GCACATTCCA GTCCATGCCC 
AACCTGATAA GTGACTCCCC TCAGGCTATG GCAAGCCTGG CAAGTGATCA CCCTCAGGCT 
GGGCCCAGCC TAATGTCTGG TCACACCCAG GCTGTGCCGA GTCTGGCAAC TTGTCCTCTG 
CAGAGCATCC CTCCAGTTTC TGACATGCAG CCAGAAACTG GGTCCAGCTC CAGTTCTGGC 
CGAACTTCAG GGAGCCTGTG TCCCAGAGAT GGGGCTGATC CCTCCCTGGA GAATGCTCTG 
TGTAAGATGG AAAGTGAGGA TTCCACTCGC TTCACTGACT TACTGGGACA AGGTCCCATA 
GTCCCCGGTC TGGATGCTCC CAAGGACTTG GCCATCCCCT CAGAACTGGA GGAGCCAATT 
AACCTCTCTG TGAAGAAACC TCCACTGGCG CCAGTGGTCA GCACGTCTAC AGCTCTGCAG 
CAGTACCAGA ACCCAAAAGG TGAGACTCAA GCAGCCTGCC CTTCATGTTG GCCACAGACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( XM_011520526.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518828.1 Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu63426
Clone ID Related Accession (Same CDS sequence)	XM_011520526.2
Accession Version	XM_011520526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTATG GCAGTACAGA TGAATGTGTG TCTCTGTGGT CACACAAAGG CTGCATAACT 
ACTGAAGGTG GACAAATGTC CAGGGCAGAT GCTCCTGCTT ATGGAGGCTT ACAGGGGTCA 
TCACCCTTTT ATCAAAGCCA CCAGTCTCCA GTGGCTCAGC AAGAGGCTCT TAGCCACCCC 
TCACACAAGT TCCAGTCTCC AGCAGTGTGC TCCTCATCTG TGTGCTGCTC CCACTGCTCC 
CCAGTCTCGC CTTCCCTCAA AGGCCAGGTC CCCCCACCCA GCATACACCC AGCCCACAGC 
TTCAGGCAGC CCCCTGAGAT GGTGCCCCAG CAGCTGGGGT CTCTGCAGTG CTCTGCCCTG 
CTGCCCAGGG AGAAAGAGCT GGCCTGCAGC CCTCATCCAC CAAAGCTGCT GCAGCCCTGG 
CTGGAAACCC AGCCCCCCGT GGAGCAGGAG AGCACATCCC AGCGGCTGGG GCAGCAGCTG 
ACTTCCCAGC CCGTGTGCAT TGTCCCCCCA CAGGATGTTC AGCAAGGAGC CCATGCCCAG 
CCCACCTTAC AGACACCCTC TATCCAAGTC CAGTTTGGCC ACCACCAGAA GCTGAAGCTC 
AGTCACTTTC AGCAGCAGCC ACAGCAGCAG CTACCACCTC CACCACCACC CCTCCCCCAT 
CCCCCACCTC CCCTTCCCCC TCCCCCACAG CAGCCACACC CACCTCTTCC TCCATCCCAG 
CATCTGGCTT CTAGTCAGCA CGAGAGCCCT CCTGGCCCTG CCTGTTCTCA GAACATGGAC 
ATAATGCATC ACAAGTTTGA GCTGGAGGAA ATGCAGAAGG ACTTGGAGCT TCTTCTCCAG 
GCTCAACAGC CCAGCCTGCA ACTGAGTCAG ACCAAATCTC CTCAGCATCT TCAGCAAACC 
ATTGTGGGGC AGATCAACTA CATCGTGAGG CAGCCAGCAC CTGTCCAGTC CCAGAGCCAG 
GAGGAGACCC TGCAGGCTAC AGATGAGCCC CCAGCATCTC AGGGCTCAAA GCCGGCTCTC 
CCTCTTGACA AGAATACTGC TGCTGCCTTG CCCCAGGCGT CTGGGGAAGA AACCCCTCTC 
AGTGTCCCCC CAGTGGACAG CACCATCCAG CACTCCTCTC CAAATGTGGT GAGAAAGCAC 
TCCACCTCGC TGAGCATCAT GGGCTTTTCC AACACTCTGG AGATGGAGTT GTCATCTACC 
AGGTTGGAGA GGCCCCTAGA GCCACAGATC CAGAGTGTGA GCAACCTGAC AGCTGGTGCC 
CCCCAGGCAG TACCAAGCCT GCTGAGTGCT CCCCCCAAAA TGGTGTCCAG CCTGACAAGT 
GTTCAAAACC AGGCCATGCC CAGCCTGACA ACCAGTCACC TACAGACTGT GCCCAGCCTT 
GTGCATAGCA CATTCCAGTC CATGCCCAAC CTGATAAGTG ACTCCCCTCA GGCTATGGCA 
AGCCTGGCAA GTGATCACCC TCAGGCTGGG CCCAGCCTAA TGTCTGGTCA CACCCAGGCT 
GTGCCGAGTC TGGCAACTTG TCCTCTGCAG AGCATCCCTC CAGTTTCTGA CATGCAGCCA 
GAAACTGGGT CCAGCTCCAG TTCTGGCCGA ACTTCAGGGA GCCTGTGTCC CAGAGATGGG 
GCTGATCCCT CCCTGGAGAA TGCTCTGTGT AAGATGGAAA GTGAGGATTC CACTCGCTTC 
ACTGACTTAC TGGGACAAGG TCCCATAGTC CCCGGTCTGG ATGCTCCCAA GGACTTGGCC 
ATCCCCTCAG AACTGGAGGA GCCAATTAAC CTCTCTGTGA AGAAACCTCC ACTGGCGCCA 
GTGGTCAGCA CGTCTACAGC TCTGCAGCAG TACCAGAACC CAAAAGAGTG TGAGAATTTT 
GAACAAGGAG CCCTAGAGCT GGATGCAAAA GAGAACCAGA GCATCAGAGC CTTCAATAGT 
GAGCATAAGA TTCCCTATGT GCGACTGGAG CGACTCAAGA TCTGTGCTGC CTCCTCAGGA 
GAGATGCCTG TGTTCAAACT GAAGCCACAG AAGAATGATC AGGATGGGAG CTTCCTGCTG 
ATCATCGAGT GTGGCACTGA GTCCTCCAGC ATGTCCATTA AGGTCAGCCA GGACAGACTG 
TCTGAGGCCA CCCAGGCCCC AGGTCTGGAG GGAAGAAAGG TCACTGTCAC TTCTTTGGCT 
GGGCAGCGGC CACCAGAAGT GGAGGGCACA TCTCCTGAAG AACACAGACT CATTCCTCGA 
ACCCCAGGAG CCAAGAAGGG CCCCCCAGCC CCAATAGAGA ATGAGGACTT CTGTGCTGTT 
TGCCTCAATG GCGGAGAGTT ACTGTGCTGT GACCGCTGCC CCAAAGTGTT CCACCTCTCC 
TGCCATGTGC CAGCCTTGCT CAGCTTCCCA GGGGGAGAGT GGGTGTGTAC CTTGTGCCGC 
AGCCTGACCC AGCCCGAGAT GGAGTACGAC TGTGAGAATG CCTGCTATAA CCAGCCTGGA 
ATGCGGGCAT CTCCTGGCCT AAGCATGTAT GACCAGAAGA AGTGTGAGAA GCTGGTATTG 
TCCTTGTGCT GCAATAACCT CAGCCTGCCC TTCCATGAAC CTGTCAGCCC CCTGGCCCGG 
CATTATTACC AGATTATCAA GAGGCCCATG GACCTGTCAA TCATCCGGAG GAAGCTGCAA 
AAGAAGGACC CAGCTCACTA TACCACCCCA GAGGAGGTGG TATCAGATGT GCGCCTCATG 
TTCTGGAACT GTGCTAAGTT CAATTATCCT GACTCCGAGG TTGCAGAGGC TGGCCGCTGC 
CTGGAAGTGT TCTTTGAGGG CTGGTTGAAG GAGATCTACC CGGAGAAACG GTTTGCCCAG 
CCAAGGCAGG AGGACTCAGA CTCCGAGGAG GTGTCTAGTG AGAGTGGATG TTCCACTCCC 
CAGGGCTTCC CGTGGCCTCC CTACATGCAG GAGGGCATCC AACCCAAGAG GCGGCGACGA 
CATATGGAGA ATGAAAGAGC AAAAAGAATG TCATTTCGCC TGGCCAACAG CATCTCTCAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518828.1
CDS	97..3102
Translation	MLYGSTDECVSLWSHKGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKMESEDSTRFTDLLGQGPIVPGLDAPKDLAIPSELEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	XM_011520526.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520526.2

ORF Insert Sequence:

ATGCTGTATG GCAGTACAGA TGAATGTGTG TCTCTGTGGT CACACAAAGG CTGCATAACT 
ACTGAAGGTG GACAAATGTC CAGGGCAGAT GCTCCTGCTT ATGGAGGCTT ACAGGGGTCA 
TCACCCTTTT ATCAAAGCCA CCAGTCTCCA GTGGCTCAGC AAGAGGCTCT TAGCCACCCC 
TCACACAAGT TCCAGTCTCC AGCAGTGTGC TCCTCATCTG TGTGCTGCTC CCACTGCTCC 
CCAGTCTCGC CTTCCCTCAA AGGCCAGGTC CCCCCACCCA GCATACACCC AGCCCACAGC 
TTCAGGCAGC CCCCTGAGAT GGTGCCCCAG CAGCTGGGGT CTCTGCAGTG CTCTGCCCTG 
CTGCCCAGGG AGAAAGAGCT GGCCTGCAGC CCTCATCCAC CAAAGCTGCT GCAGCCCTGG 
CTGGAAACCC AGCCCCCCGT GGAGCAGGAG AGCACATCCC AGCGGCTGGG GCAGCAGCTG 
ACTTCCCAGC CCGTGTGCAT TGTCCCCCCA CAGGATGTTC AGCAAGGAGC CCATGCCCAG 
CCCACCTTAC AGACACCCTC TATCCAAGTC CAGTTTGGCC ACCACCAGAA GCTGAAGCTC 
AGTCACTTTC AGCAGCAGCC ACAGCAGCAG CTACCACCTC CACCACCACC CCTCCCCCAT 
CCCCCACCTC CCCTTCCCCC TCCCCCACAG CAGCCACACC CACCTCTTCC TCCATCCCAG 
CATCTGGCTT CTAGTCAGCA CGAGAGCCCT CCTGGCCCTG CCTGTTCTCA GAACATGGAC 
ATAATGCATC ACAAGTTTGA GCTGGAGGAA ATGCAGAAGG ACTTGGAGCT TCTTCTCCAG 
GCTCAACAGC CCAGCCTGCA ACTGAGTCAG ACCAAATCTC CTCAGCATCT TCAGCAAACC 
ATTGTGGGGC AGATCAACTA CATCGTGAGG CAGCCAGCAC CTGTCCAGTC CCAGAGCCAG 
GAGGAGACCC TGCAGGCTAC AGATGAGCCC CCAGCATCTC AGGGCTCAAA GCCGGCTCTC 
CCTCTTGACA AGAATACTGC TGCTGCCTTG CCCCAGGCGT CTGGGGAAGA AACCCCTCTC 
AGTGTCCCCC CAGTGGACAG CACCATCCAG CACTCCTCTC CAAATGTGGT GAGAAAGCAC 
TCCACCTCGC TGAGCATCAT GGGCTTTTCC AACACTCTGG AGATGGAGTT GTCATCTACC 
AGGTTGGAGA GGCCCCTAGA GCCACAGATC CAGAGTGTGA GCAACCTGAC AGCTGGTGCC 
CCCCAGGCAG TACCAAGCCT GCTGAGTGCT CCCCCCAAAA TGGTGTCCAG CCTGACAAGT 
GTTCAAAACC AGGCCATGCC CAGCCTGACA ACCAGTCACC TACAGACTGT GCCCAGCCTT 
GTGCATAGCA CATTCCAGTC CATGCCCAAC CTGATAAGTG ACTCCCCTCA GGCTATGGCA 
AGCCTGGCAA GTGATCACCC TCAGGCTGGG CCCAGCCTAA TGTCTGGTCA CACCCAGGCT 
GTGCCGAGTC TGGCAACTTG TCCTCTGCAG AGCATCCCTC CAGTTTCTGA CATGCAGCCA 
GAAACTGGGT CCAGCTCCAG TTCTGGCCGA ACTTCAGGGA GCCTGTGTCC CAGAGATGGG 
GCTGATCCCT CCCTGGAGAA TGCTCTGTGT AAGATGGAAA GTGAGGATTC CACTCGCTTC 
ACTGACTTAC TGGGACAAGG TCCCATAGTC CCCGGTCTGG ATGCTCCCAA GGACTTGGCC 
ATCCCCTCAG AACTGGAGGA GCCAATTAAC CTCTCTGTGA AGAAACCTCC ACTGGCGCCA 
GTGGTCAGCA CGTCTACAGC TCTGCAGCAG TACCAGAACC CAAAAGAGTG TGAGAATTTT 
GAACAAGGAG CCCTAGAGCT GGATGCAAAA GAGAACCAGA GCATCAGAGC CTTCAATAGT 
GAGCATAAGA TTCCCTATGT GCGACTGGAG CGACTCAAGA TCTGTGCTGC CTCCTCAGGA 
GAGATGCCTG TGTTCAAACT GAAGCCACAG AAGAATGATC AGGATGGGAG CTTCCTGCTG 
ATCATCGAGT GTGGCACTGA GTCCTCCAGC ATGTCCATTA AGGTCAGCCA GGACAGACTG 
TCTGAGGCCA CCCAGGCCCC AGGTCTGGAG GGAAGAAAGG TCACTGTCAC TTCTTTGGCT 
GGGCAGCGGC CACCAGAAGT GGAGGGCACA TCTCCTGAAG AACACAGACT CATTCCTCGA 
ACCCCAGGAG CCAAGAAGGG CCCCCCAGCC CCAATAGAGA ATGAGGACTT CTGTGCTGTT 
TGCCTCAATG GCGGAGAGTT ACTGTGCTGT GACCGCTGCC CCAAAGTGTT CCACCTCTCC 
TGCCATGTGC CAGCCTTGCT CAGCTTCCCA GGGGGAGAGT GGGTGTGTAC CTTGTGCCGC 
AGCCTGACCC AGCCCGAGAT GGAGTACGAC TGTGAGAATG CCTGCTATAA CCAGCCTGGA 
ATGCGGGCAT CTCCTGGCCT AAGCATGTAT GACCAGAAGA AGTGTGAGAA GCTGGTATTG 
TCCTTGTGCT GCAATAACCT CAGCCTGCCC TTCCATGAAC CTGTCAGCCC CCTGGCCCGG 
CATTATTACC AGATTATCAA GAGGCCCATG GACCTGTCAA TCATCCGGAG GAAGCTGCAA 
AAGAAGGACC CAGCTCACTA TACCACCCCA GAGGAGGTGG TATCAGATGT GCGCCTCATG 
TTCTGGAACT GTGCTAAGTT CAATTATCCT GACTCCGAGG TTGCAGAGGC TGGCCGCTGC 
CTGGAAGTGT TCTTTGAGGG CTGGTTGAAG GAGATCTACC CGGAGAAACG GTTTGCCCAG 
CCAAGGCAGG AGGACTCAGA CTCCGAGGAG GTGTCTAGTG AGAGTGGATG TTCCACTCCC 
CAGGGCTTCC CGTGGCCTCC CTACATGCAG GAGGGCATCC AACCCAAGAG GCGGCGACGA 
CATATGGAGA ATGAAAGAGC AAAAAGAATG TCATTTCGCC TGGCCAACAG CATCTCTCAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM66 ( NM_014818.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_055633.1 Homo sapiens tripartite motif containing 66 (TRIM66), mRNA.

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CloneID	OHu17490
Clone ID Related Accession (Same CDS sequence)	NM_014818.1
Accession Version	NM_014818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	tripartite motif-containing protein 66
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB002296.2 and AC104360.8. On or before Oct 31, 2008 this sequence version replaced XM_001716253.1, XM_001717903.1, XM_001716830.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END##

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GGTAAAACTG 
GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCACTGG CGCCAGTGGT CAGCACGTCT 
ACAGCTCTGC AGCAGTACCA GAACCCAAAA GAGTGTGAGA ATTTTGAACA AGGAGCCCTA 
GAGCTGGATG CAAAAGAGAA CCAGAGCATC AGAGCCTTCA ATAGTGAGCA TAAGATTCCC 
TATGTGCGAC TGGAGCGACT CAAGATCTGT GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC 
AAACTGAAGC CACAGAAGAA TGATCAGGAT GGGAGCTTCC TGCTGATCAT CGAGTGTGGC 
ACTGAGTCCT CCAGCATGTC CATTAAGGTC AGCCAGGACA GACTGTCTGA GGCCACCCAG 
GCCCCAGGTC TGGAGGGAAG AAAGGTCACT GTCACTTCTT TGGCTGGGCA GCGGCCACCA 
GAAGTGGAGG GCACATCTCC TGAAGAACAC AGACTCATTC CTCGAACCCC AGGAGCCAAG 
AAGGGCCCCC CAGCCCCAAT AGAGAATGAG GACTTCTGTG CTGTTTGCCT CAATGGCGGA 
GAGTTACTGT GCTGTGACCG CTGCCCCAAA GTGTTCCACC TCTCCTGCCA TGTGCCAGCC 
TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG TGTACCTTGT GCCGCAGCCT GACCCAGCCC 
GAGATGGAGT ACGACTGTGA GAATGCCTGC TATAACCAGC CTGGAATGCG GGCATCTCCT 
GGCCTAAGCA TGTATGACCA GAAGAAGTGT GAGAAGCTGG TATTGTCCTT GTGCTGCAAT 
AACCTCAGCC TGCCCTTCCA TGAACCTGTC AGCCCCCTGG CCCGGCATTA TTACCAGATT 
ATCAAGAGGC CCATGGACCT GTCAATCATC CGGAGGAAGC TGCAAAAGAA GGACCCAGCT 
CACTATACCA CCCCAGAGGA GGTGGTATCA GATGTGCGCC TCATGTTCTG GAACTGTGCT 
AAGTTCAATT ATCCTGACTC CGAGGTTGCA GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT 
GAGGGCTGGT TGAAGGAGAT CTACCCGGAG AAACGGTTTG CCCAGCCAAG GCAGGAGGAC 
TCAGACTCCG AGGAGGTGTC TAGTGAGAGT GGATGTTCCA CTCCCCAGGG CTTCCCGTGG 
CCTCCCTACA TGCAGGAGGG CATCCAACCC AAGAGGCGGC GACGACATAT GGAGAATGAA 
AGAGCAAAAA GAATGTCATT TCGCCTGGCC AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055633.1
CDS	196..3846
Translation	MARNCSECKEKRAAHILCTYCNRWLCSSCTEEHRHSPVPGGPFFPRAQKGSPGVNGGPGDFTLYCPLHTQEVLKLFCETCDMLTCHSCLVVEHKEHRCRHVEEVLQNQRMLLEGVTTQVAHKKSSLQTSAKQIEDRIFEVKHQHRKVENQIKMAKMVLMNELNKQANGLIEELEGITNERKRKLEQQLQSIMVLNRQFEHVQNFINWAVCSKTSVPFLFSKELIVFQMQRLLETSCNTDPGSPWSIRFTWEPNFWTKQLASLGCITTEGGQMSRADAPAYGGLQGSSPFYQSHQSPVAQQEALSHPSHKFQSPAVCSSSVCCSHCSPVSPSLKGQVPPPSIHPAHSFRQPPEMVPQQLGSLQCSALLPREKELACSPHPPKLLQPWLETQPPVEQESTSQRLGQQLTSQPVCIVPPQDVQQGAHAQPTLQTPSIQVQFGHHQKLKLSHFQQQPQQQLPPPPPPLPHPPPPLPPPPQQPHPPLPPSQHLASSQHESPPGPACSQNMDIMHHKFELEEMQKDLELLLQAQQPSLQLSQTKSPQHLQQTIVGQINYIVRQPAPVQSQSQEETLQATDEPPASQGSKPALPLDKNTAAALPQASGEETPLSVPPVDSTIQHSSPNVVRKHSTSLSIMGFSNTLEMELSSTRLERPLEPQIQSVSNLTAGAPQAVPSLLSAPPKMVSSLTSVQNQAMPSLTTSHLQTVPSLVHSTFQSMPNLISDSPQAMASLASDHPQAGPSLMSGHTQAVPSLATCPLQSIPPVSDMQPETGSSSSSGRTSGSLCPRDGADPSLENALCKVKLEEPINLSVKKPPLAPVVSTSTALQQYQNPKECENFEQGALELDAKENQSIRAFNSEHKIPYVRLERLKICAASSGEMPVFKLKPQKNDQDGSFLLIIECGTESSSMSIKVSQDRLSEATQAPGLEGRKVTVTSLAGQRPPEVEGTSPEEHRLIPRTPGAKKGPPAPIENEDFCAVCLNGGELLCCDRCPKVFHLSCHVPALLSFPGGEWVCTLCRSLTQPEMEYDCENACYNQPGMRASPGLSMYDQKKCEKLVLSLCCNNLSLPFHEPVSPLARHYYQIIKRPMDLSIIRRKLQKKDPAHYTTPEEVVSDVRLMFWNCAKFNYPDSEVAEAGRCLEVFFEGWLKEIYPEKRFAQPRQEDSDSEEVSSESGCSTPQGFPWPPYMQEGIQPKRRRRHMENERAKRMSFRLANSISQV

Target ORF information:

RefSeq Version	NM_014818.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 66 (TRIM66), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014818.1

ORF Insert Sequence:

ATGGCCAGGA ACTGCTCTGA GTGCAAGGAG AAGAGGGCAG CACATATCCT CTGCACCTAC 
TGCAATCGCT GGCTGTGCAG CTCTTGCACA GAGGAACACC GACACAGCCC TGTCCCCGGG 
GGCCCATTCT TTCCTCGGGC CCAGAAGGGA TCTCCAGGAG TGAATGGTGG TCCCGGAGAC 
TTCACCTTGT ATTGTCCTCT ACACACACAG GAAGTACTCA AGCTATTCTG TGAGACATGT 
GATATGCTCA CTTGCCATAG CTGCCTAGTG GTGGAACACA AAGAACACAG GTGCAGACAT 
GTTGAAGAAG TTTTGCAAAA CCAGAGGATG CTTCTGGAAG GTGTGACTAC ACAGGTGGCA 
CATAAGAAAT CCAGTCTACA GACATCTGCA AAGCAAATTG AGGACAGGAT TTTTGAAGTG 
AAGCATCAGC ATAGGAAGGT GGAAAACCAG ATCAAAATGG CCAAGATGGT TCTGATGAAT 
GAGCTGAACA AACAGGCCAA TGGGCTAATA GAGGAATTAG AGGGGATTAC TAATGAGAGA 
AAGCGGAAGC TGGAACAGCA GTTACAGAGC ATCATGGTTC TCAACCGTCA GTTTGAGCAT 
GTGCAGAATT TCATCAACTG GGCTGTCTGC AGCAAAACCA GTGTCCCTTT TCTTTTCAGC 
AAAGAGCTGA TTGTGTTTCA GATGCAGCGA TTGCTGGAGA CAAGTTGTAA CACAGATCCT 
GGCTCCCCTT GGAGTATCAG ATTCACCTGG GAGCCTAACT TCTGGACCAA GCAGCTAGCT 
TCTCTTGGCT GCATAACTAC TGAAGGTGGA CAAATGTCCA GGGCAGATGC TCCTGCTTAT 
GGAGGCTTAC AGGGGTCATC ACCCTTTTAT CAAAGCCACC AGTCTCCAGT GGCTCAGCAA 
GAGGCTCTTA GCCACCCCTC ACACAAGTTC CAGTCTCCAG CAGTGTGCTC CTCATCTGTG 
TGCTGCTCCC ACTGCTCCCC AGTCTCGCCT TCCCTCAAAG GCCAGGTCCC CCCACCCAGC 
ATACACCCAG CCCACAGCTT CAGGCAGCCC CCTGAGATGG TGCCCCAGCA GCTGGGGTCT 
CTGCAGTGCT CTGCCCTGCT GCCCAGGGAG AAAGAGCTGG CCTGCAGCCC TCATCCACCA 
AAGCTGCTGC AGCCCTGGCT GGAAACCCAG CCCCCCGTGG AGCAGGAGAG CACATCCCAG 
CGGCTGGGGC AGCAGCTGAC TTCCCAGCCC GTGTGCATTG TCCCCCCACA GGATGTTCAG 
CAAGGAGCCC ATGCCCAGCC CACCTTACAG ACACCCTCTA TCCAAGTCCA GTTTGGCCAC 
CACCAGAAGC TGAAGCTCAG TCACTTTCAG CAGCAGCCAC AGCAGCAGCT ACCACCTCCA 
CCACCACCCC TCCCCCATCC CCCACCTCCC CTTCCCCCTC CCCCACAGCA GCCACACCCA 
CCTCTTCCTC CATCCCAGCA TCTGGCTTCT AGTCAGCACG AGAGCCCTCC TGGCCCTGCC 
TGTTCTCAGA ACATGGACAT AATGCATCAC AAGTTTGAGC TGGAGGAAAT GCAGAAGGAC 
TTGGAGCTTC TTCTCCAGGC TCAACAGCCC AGCCTGCAAC TGAGTCAGAC CAAATCTCCT 
CAGCATCTTC AGCAAACCAT TGTGGGGCAG ATCAACTACA TCGTGAGGCA GCCAGCACCT 
GTCCAGTCCC AGAGCCAGGA GGAGACCCTG CAGGCTACAG ATGAGCCCCC AGCATCTCAG 
GGCTCAAAGC CGGCTCTCCC TCTTGACAAG AATACTGCTG CTGCCTTGCC CCAGGCGTCT 
GGGGAAGAAA CCCCTCTCAG TGTCCCCCCA GTGGACAGCA CCATCCAGCA CTCCTCTCCA 
AATGTGGTGA GAAAGCACTC CACCTCGCTG AGCATCATGG GCTTTTCCAA CACTCTGGAG 
ATGGAGTTGT CATCTACCAG GTTGGAGAGG CCCCTAGAGC CACAGATCCA GAGTGTGAGC 
AACCTGACAG CTGGTGCCCC CCAGGCAGTA CCAAGCCTGC TGAGTGCTCC CCCCAAAATG 
GTGTCCAGCC TGACAAGTGT TCAAAACCAG GCCATGCCCA GCCTGACAAC CAGTCACCTA 
CAGACTGTGC CCAGCCTTGT GCATAGCACA TTCCAGTCCA TGCCCAACCT GATAAGTGAC 
TCCCCTCAGG CTATGGCAAG CCTGGCAAGT GATCACCCTC AGGCTGGGCC CAGCCTAATG 
TCTGGTCACA CCCAGGCTGT GCCGAGTCTG GCAACTTGTC CTCTGCAGAG CATCCCTCCA 
GTTTCTGACA TGCAGCCAGA AACTGGGTCC AGCTCCAGTT CTGGCCGAAC TTCAGGGAGC 
CTGTGTCCCA GAGATGGGGC TGATCCCTCC CTGGAGAATG CTCTGTGTAA GGTAAAACTG 
GAGGAGCCAA TTAACCTCTC TGTGAAGAAA CCTCCACTGG CGCCAGTGGT CAGCACGTCT 
ACAGCTCTGC AGCAGTACCA GAACCCAAAA GAGTGTGAGA ATTTTGAACA AGGAGCCCTA 
GAGCTGGATG CAAAAGAGAA CCAGAGCATC AGAGCCTTCA ATAGTGAGCA TAAGATTCCC 
TATGTGCGAC TGGAGCGACT CAAGATCTGT GCTGCCTCCT CAGGAGAGAT GCCTGTGTTC 
AAACTGAAGC CACAGAAGAA TGATCAGGAT GGGAGCTTCC TGCTGATCAT CGAGTGTGGC 
ACTGAGTCCT CCAGCATGTC CATTAAGGTC AGCCAGGACA GACTGTCTGA GGCCACCCAG 
GCCCCAGGTC TGGAGGGAAG AAAGGTCACT GTCACTTCTT TGGCTGGGCA GCGGCCACCA 
GAAGTGGAGG GCACATCTCC TGAAGAACAC AGACTCATTC CTCGAACCCC AGGAGCCAAG 
AAGGGCCCCC CAGCCCCAAT AGAGAATGAG GACTTCTGTG CTGTTTGCCT CAATGGCGGA 
GAGTTACTGT GCTGTGACCG CTGCCCCAAA GTGTTCCACC TCTCCTGCCA TGTGCCAGCC 
TTGCTCAGCT TCCCAGGGGG AGAGTGGGTG TGTACCTTGT GCCGCAGCCT GACCCAGCCC 
GAGATGGAGT ACGACTGTGA GAATGCCTGC TATAACCAGC CTGGAATGCG GGCATCTCCT 
GGCCTAAGCA TGTATGACCA GAAGAAGTGT GAGAAGCTGG TATTGTCCTT GTGCTGCAAT 
AACCTCAGCC TGCCCTTCCA TGAACCTGTC AGCCCCCTGG CCCGGCATTA TTACCAGATT 
ATCAAGAGGC CCATGGACCT GTCAATCATC CGGAGGAAGC TGCAAAAGAA GGACCCAGCT 
CACTATACCA CCCCAGAGGA GGTGGTATCA GATGTGCGCC TCATGTTCTG GAACTGTGCT 
AAGTTCAATT ATCCTGACTC CGAGGTTGCA GAGGCTGGCC GCTGCCTGGA AGTGTTCTTT 
GAGGGCTGGT TGAAGGAGAT CTACCCGGAG AAACGGTTTG CCCAGCCAAG GCAGGAGGAC 
TCAGACTCCG AGGAGGTGTC TAGTGAGAGT GGATGTTCCA CTCCCCAGGG CTTCCCGTGG 
CCTCCCTACA TGCAGGAGGG CATCCAACCC AAGAGGCGGC GACGACATAT GGAGAATGAA 
AGAGCAAAAA GAATGTCATT TCGCCTGGCC AACAGCATCT CTCAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TRIM66
Entrez Gene ID	9866
Full Name	tripartite motif containing 66
Synonyms	C11orf29,TIF1D,TIF1DELTA
General protein information	Preferred Names tripartite motif containing 66 Names tripartite motif-containing protein 66 transcriptional intermediary factor 1 delta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.4
Disorder MIM:	612000

Canis lupus familiaris (dog)	TRIM66	XP_005633693.1
Bos taurus (cattle)	TRIM66	XP_002693153.3
Mus musculus (house mouse)	Trim66	NP_001164383.1
Gallus gallus (chicken)	TRIM66	XP_004941449.1
Xenopus tropicalis (tropical clawed frog)	trim66	XP_002942909.2
Homo sapiens (human)	TRIM66	NP_055633.1
Rattus norvegicus (Norway rat)	Trim66	NP_001178768.1
	TRIM66	XP_001097566.2
Pan troglodytes (chimpanzee)	TRIM66	XP_003313014.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TRIM66 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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