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RNF10 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol RNF10
Entrez Gene ID 9921
Full Name ring finger protein 10
Synonyms RIE2
General protein information
Preferred Names

ring finger protein 10

Names

RING finger protein 10
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

12

12q24.31
Summary The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known. [provided by RefSeq, Jul 2008].
Disorder MIM:

615998


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017020283.1 XP_016875772.1 RING finger protein 10 isoform X4
NM_001330474.1 NP_001317403.1 RING finger protein 10 isoform 2
NM_001330474.2 NP_001317403.1 RING finger protein 10 isoform 2
XM_017020283.2 XP_016875772.1 RING finger protein 10 isoform X3
NM_014868.4 NP_055683.3 RING finger protein 10 isoform 1
XM_005254013.2 XP_005254070.1 RING finger protein 10 isoform X1
XM_017020282.1 XP_016875771.1 RING finger protein 10 isoform X2
NM_014868.5 NP_055683.3 RING finger protein 10 isoform 1

Pathways from BioSystems


Homologies


Drosophila melanogaster (fruit fly) CG12099 NP_647623.1
Bos taurus (cattle) RNF10 NP_001070337.1
Rattus norvegicus (Norway rat) Rnf10 NP_001011904.1
Homo sapiens (human) RNF10 NP_055683.3
Canis lupus familiaris (dog) RNF10 XP_851332.2
Danio rerio (zebrafish) rnf10 NP_001116727.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007954 XP_317518.4
RNF10 XP_002798843.1
Mus musculus (house mouse) Rnf10 NP_057907.2
Gallus gallus (chicken) RNF10 XP_415267.2
Xenopus tropicalis (tropical clawed frog) rnf10 NP_989388.1
Pan troglodytes (chimpanzee) RNF10 XP_001161754.2

Gene Ontology


Bibliography

Related articles in PubMed

Whole exome sequencing identifies variation in CYB5A and RNF10 associated with adiposity and type 2 diabetes.
Huang K, Nair AK, Muller YL, Piaggi P, Bian L, Del Rosario M, Knowler WC, Kobes S, Hanson RL, Bogardus C, Baier LJ
Obesity (Silver Spring, Md.)22(4)984-8(2014 Apr)

Protein microarray characterization of the S-nitrosoproteome.
Lee YI, Giovinazzo D, Kang HC, Lee Y, Jeong JS, Doulias PT, Xie Z, Hu J, Ghasemi M, Ischiropoulos H, Qian J, Zhu H, Blackshaw S, Dawson VL, Dawson TM
Molecular & cellular proteomics : MCP13(1)63-72(2014 Jan)

New susceptibility loci associated with kidney disease in type 1 diabetes.
Sandholm N, Salem RM, McKnight AJ, Brennan EP, Forsblom C, Isakova T, McKay GJ, Williams WW, Sadlier DM, M?kinen VP, Swan EJ, Palmer C, Boright AP, Ahlqvist E, Deshmukh HA, Keller BJ, Huang H, Ahola AJ, Fagerholm E, Gordin D, Harjutsalo V, He B, Heikkil? O, Hietala K, Kyt? J, Lahermo P, Lehto M, Lithovius R, Osterholm AM, Parkkonen M, Pitk?niemi J, Roseng?rd-B?rlund M, Saraheimo M, Sarti C, S?derlund J, Soro-Paavonen A, Syreeni A, Thorn LM, Tikkanen H, Tolonen N, Tryggvason K, Tuomilehto J, Wad?n J, Gill GV, Prior S, Guiducci C, Mirel DB, Taylor A, Hosseini SM, , Parving HH, Rossing P, Tarnow L, Ladenvall C, Alhenc-Gelas F, Lefebvre P, Rigalleau V, Roussel R, Tregouet DA, Maestroni A, Maestroni S, Falhammar H, Gu T, M?llsten A, Cimponeriu D, Ioana M, Mota M, Mota E, Serafinceanu C, Stavarachi M, Hanson RL, Nelson RG, Kretzler M, Colhoun HM, Panduru NM, Gu HF, Brismar K, Zerbini G, Hadjadj S, Marre M, Groop L, Lajer M, Bull SB, Waggott D, Paterson AD, Savage DA, Bain SC, Martin F, Hirschhorn JN, Godson C, Florez JC, Groop PH, Maxwell AP
PLoS genetics8(9)e1002921(2012 Sep)

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
Lee OH, Kim H, He Q, Baek HJ, Yang D, Chen LY, Liang J, Chae HK, Safari A, Liu D, Songyang Z
Molecular & cellular proteomics : MCP10(2)M110.001628(2011 Feb)

A novel function of RING finger protein 10 in transcriptional regulation of the myelin-associated glycoprotein gene and myelin formation in Schwann cells.
Hoshikawa S, Ogata T, Fujiwara S, Nakamura K, Tanaka S
PloS one3(10)e3464(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CYB5A, which has a role in stearyl-CoA-desaturase activity, and RNF10, with an unknown role in weight regulating pathways, associated with adiposity and nominally increased the risk for T2D in American Indians.
Title: Whole exome sequencing identifies variation in CYB5A and RNF10 associated with adiposity and type 2 diabetes.

RNF10 is a trans-acting protein regulating MAG expression and is required for myelin formation.
Title: A novel function of RING finger protein 10 in transcriptional regulation of the myelin-associated glycoprotein gene and myelin formation in Schwann cells.

MEOX2 and Meox2 binding to RNF10 protein was characterized.
Title: Characterization of Mesenchyme Homeobox 2 (MEOX2) transcription factor binding to RING finger protein 10.

The following RNF10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu31406 NM_014868.5
Latest version!		 Homo sapiens ring finger protein 10 (RNF10), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
OHu47004 NM_001330474.2
Latest version!		 Homo sapiens ring finger protein 10 (RNF10), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu47005 XM_005254013.2
Latest version!		 Homo sapiens ring finger protein 10 (RNF10), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu84364 XM_017020282.1
Latest version!		 Homo sapiens ring finger protein 10 (RNF10), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu84365 XM_017020283.2
Latest version!		 Homo sapiens ring finger protein 10 (RNF10), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu47004 NM_001330474.1 Homo sapiens ring finger protein 10 (RNF10), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu84365 XM_017020283.1 Homo sapiens ring finger protein 10 (RNF10), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu31406 NM_014868.4 Homo sapiens ring finger protein 10 (RNF10), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu31406
Clone ID Related Accession (Same CDS sequence) NM_014868.4 , NM_014868.5
Accession Version NM_014868.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-01
Organism Homo sapiens(human)
Product RING finger protein 10 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA103961.1, BC016622.1, DB526099.1 and BQ015862.1. On Aug 31, 2007 this sequence version replaced NM_014868.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.18609.1, SRR1660809.29793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGGGTGTG 
CTGGAGTATC TGTCTGCCTT CGATGAAGAA ACCACGGAAG TTTGTTCTCT GGACACTCCT 
TCTAGACCTC TTGCTCTCCC TCTGGTAGAA GAGGAGGAAG CAGTGTCTGA ACCAGAGCCT 
GAGGGGTTGC CAGAGGCCTG TGATGACTTG GAGTTAGCAG ATGACAATCT TAAAGAGGGG 
ACCATTTGCA CTGAGTCCAG CCAGCAGGAA CCCATCACCA AGTCAGGCTT CACACGCCTC 
AGCAGCTCTC CTTGTTACTA CTTTTACCAA GCGGAAGATG GACAGCATAT GTTCCTGCAC 
CCTGTGAATG TGCGCTGCCT CGTGCGGGAG TACGGCAGCC TGGAGAGGAG CCCCGAGAAG 
ATCTCAGCAA CTGTGGTGGA GATTGCTGGC TACTCCATGT CTGAGGATGT TCGACAGCGT 
CACAGATATC TCTCTCACTT GCCACTCACC TGTGAGTTCA GCATCTGTGA ACTGGCTTTG 
CAACCTCCTG TGGTCTCTAA GGAAACCCTA GAGATGTTCT CAGATGACAT TGAGAAGAGG 
AAACGTCAGC GCCAAAAGAA GGCTCGGGAG GAACGCCGCC GAGAGCGCAG GATTGAGATA 
GAGGAGAACA AGAAACAGGG CAAGTACCCA GAAGTCCACA TTCCCCTCGA GAATCTACAG 
CAGTTTCCTG CCTTCAATTC TTATACCTGC TCCTCTGATT CTGCTTTGGG TCCCACCAGC 
ACCGAGGGCC ATGGGGCCCT CTCCATTTCT CCTCTCAGCA GAAGTCCAGG TTCCCATGCA 
GACTTTCTGC TGACCCCTCT GTCACCCACT GCCAGTCAGG GCAGTCCCTC ATTCTGCGTT 
GGGAGTCTGG AAGAAGACTC TCCCTTCCCT TCCTTTGCCC AGATGCTGAG GGTTGGAAAA 
GCAAAAGCAG ATGTGTGGCC CAAAACTGCT CCAAAGAAAG ATGAGAACAG CTTAGTTCCT 
CCTGCCCCTG TGGACAGCGA CGGGGAGAGT GATAATTCAG ACCGTGTTCC TGTGCCCAGT 
TTTCAAAATT CCTTCAGCCA AGCTATTGAA GCAGCCTTCA TGAAACTGGA CACACCAGCT 
ACTTCAGATC CCCTCTCTGA AGAGAAAGGA GGAAAGAAAA GAAAAAAACA GAAACAGAAG 
CTCCTGTTCA GCACCTCAGT CGTCCACACC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055683.3
CDS484..2919
Translation

Target ORF information:

RefSeq Version NM_014868.4
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014868.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGGGTGTG 
CTGGAGTATC TGTCTGCCTT CGATGAAGAA ACCACGGAAG TTTGTTCTCT GGACACTCCT 
TCTAGACCTC TTGCTCTCCC TCTGGTAGAA GAGGAGGAAG CAGTGTCTGA ACCAGAGCCT 
GAGGGGTTGC CAGAGGCCTG TGATGACTTG GAGTTAGCAG ATGACAATCT TAAAGAGGGG 
ACCATTTGCA CTGAGTCCAG CCAGCAGGAA CCCATCACCA AGTCAGGCTT CACACGCCTC 
AGCAGCTCTC CTTGTTACTA CTTTTACCAA GCGGAAGATG GACAGCATAT GTTCCTGCAC 
CCTGTGAATG TGCGCTGCCT CGTGCGGGAG TACGGCAGCC TGGAGAGGAG CCCCGAGAAG 
ATCTCAGCAA CTGTGGTGGA GATTGCTGGC TACTCCATGT CTGAGGATGT TCGACAGCGT 
CACAGATATC TCTCTCACTT GCCACTCACC TGTGAGTTCA GCATCTGTGA ACTGGCTTTG 
CAACCTCCTG TGGTCTCTAA GGAAACCCTA GAGATGTTCT CAGATGACAT TGAGAAGAGG 
AAACGTCAGC GCCAAAAGAA GGCTCGGGAG GAACGCCGCC GAGAGCGCAG GATTGAGATA 
GAGGAGAACA AGAAACAGGG CAAGTACCCA GAAGTCCACA TTCCCCTCGA GAATCTACAG 
CAGTTTCCTG CCTTCAATTC TTATACCTGC TCCTCTGATT CTGCTTTGGG TCCCACCAGC 
ACCGAGGGCC ATGGGGCCCT CTCCATTTCT CCTCTCAGCA GAAGTCCAGG TTCCCATGCA 
GACTTTCTGC TGACCCCTCT GTCACCCACT GCCAGTCAGG GCAGTCCCTC ATTCTGCGTT 
GGGAGTCTGG AAGAAGACTC TCCCTTCCCT TCCTTTGCCC AGATGCTGAG GGTTGGAAAA 
GCAAAAGCAG ATGTGTGGCC CAAAACTGCT CCAAAGAAAG ATGAGAACAG CTTAGTTCCT 
CCTGCCCCTG TGGACAGCGA CGGGGAGAGT GATAATTCAG ACCGTGTTCC TGTGCCCAGT 
TTTCAAAATT CCTTCAGCCA AGCTATTGAA GCAGCCTTCA TGAAACTGGA CACACCAGCT 
ACTTCAGATC CCCTCTCTGA AGAGAAAGGA GGAAAGAAAA GAAAAAAACA GAAACAGAAG 
CTCCTGTTCA GCACCTCAGT CGTCCACACC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu31406
Clone ID Related Accession (Same CDS sequence) NM_014868.4 , NM_014868.5
Accession Version NM_014868.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-16
Organism Homo sapiens(human)
Product RING finger protein 10 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA103961.1, BC016622.1, DB526099.1 and BQ015862.1. On Nov 22, 2018 this sequence version replaced NM_014868.4. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660807.177399.1, SRR1660807.18609.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000325954.9/ ENSP00000322242.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGGGTGTG 
CTGGAGTATC TGTCTGCCTT CGATGAAGAA ACCACGGAAG TTTGTTCTCT GGACACTCCT 
TCTAGACCTC TTGCTCTCCC TCTGGTAGAA GAGGAGGAAG CAGTGTCTGA ACCAGAGCCT 
GAGGGGTTGC CAGAGGCCTG TGATGACTTG GAGTTAGCAG ATGACAATCT TAAAGAGGGG 
ACCATTTGCA CTGAGTCCAG CCAGCAGGAA CCCATCACCA AGTCAGGCTT CACACGCCTC 
AGCAGCTCTC CTTGTTACTA CTTTTACCAA GCGGAAGATG GACAGCATAT GTTCCTGCAC 
CCTGTGAATG TGCGCTGCCT CGTGCGGGAG TACGGCAGCC TGGAGAGGAG CCCCGAGAAG 
ATCTCAGCAA CTGTGGTGGA GATTGCTGGC TACTCCATGT CTGAGGATGT TCGACAGCGT 
CACAGATATC TCTCTCACTT GCCACTCACC TGTGAGTTCA GCATCTGTGA ACTGGCTTTG 
CAACCTCCTG TGGTCTCTAA GGAAACCCTA GAGATGTTCT CAGATGACAT TGAGAAGAGG 
AAACGTCAGC GCCAAAAGAA GGCTCGGGAG GAACGCCGCC GAGAGCGCAG GATTGAGATA 
GAGGAGAACA AGAAACAGGG CAAGTACCCA GAAGTCCACA TTCCCCTCGA GAATCTACAG 
CAGTTTCCTG CCTTCAATTC TTATACCTGC TCCTCTGATT CTGCTTTGGG TCCCACCAGC 
ACCGAGGGCC ATGGGGCCCT CTCCATTTCT CCTCTCAGCA GAAGTCCAGG TTCCCATGCA 
GACTTTCTGC TGACCCCTCT GTCACCCACT GCCAGTCAGG GCAGTCCCTC ATTCTGCGTT 
GGGAGTCTGG AAGAAGACTC TCCCTTCCCT TCCTTTGCCC AGATGCTGAG GGTTGGAAAA 
GCAAAAGCAG ATGTGTGGCC CAAAACTGCT CCAAAGAAAG ATGAGAACAG CTTAGTTCCT 
CCTGCCCCTG TGGACAGCGA CGGGGAGAGT GATAATTCAG ACCGTGTTCC TGTGCCCAGT 
TTTCAAAATT CCTTCAGCCA AGCTATTGAA GCAGCCTTCA TGAAACTGGA CACACCAGCT 
ACTTCAGATC CCCTCTCTGA AGAGAAAGGA GGAAAGAAAA GAAAAAAACA GAAACAGAAG 
CTCCTGTTCA GCACCTCAGT CGTCCACACC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_055683.3
CDS457..2892
Translation

Target ORF information:

RefSeq Version NM_014868.5
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_014868.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGGGTGTG 
CTGGAGTATC TGTCTGCCTT CGATGAAGAA ACCACGGAAG TTTGTTCTCT GGACACTCCT 
TCTAGACCTC TTGCTCTCCC TCTGGTAGAA GAGGAGGAAG CAGTGTCTGA ACCAGAGCCT 
GAGGGGTTGC CAGAGGCCTG TGATGACTTG GAGTTAGCAG ATGACAATCT TAAAGAGGGG 
ACCATTTGCA CTGAGTCCAG CCAGCAGGAA CCCATCACCA AGTCAGGCTT CACACGCCTC 
AGCAGCTCTC CTTGTTACTA CTTTTACCAA GCGGAAGATG GACAGCATAT GTTCCTGCAC 
CCTGTGAATG TGCGCTGCCT CGTGCGGGAG TACGGCAGCC TGGAGAGGAG CCCCGAGAAG 
ATCTCAGCAA CTGTGGTGGA GATTGCTGGC TACTCCATGT CTGAGGATGT TCGACAGCGT 
CACAGATATC TCTCTCACTT GCCACTCACC TGTGAGTTCA GCATCTGTGA ACTGGCTTTG 
CAACCTCCTG TGGTCTCTAA GGAAACCCTA GAGATGTTCT CAGATGACAT TGAGAAGAGG 
AAACGTCAGC GCCAAAAGAA GGCTCGGGAG GAACGCCGCC GAGAGCGCAG GATTGAGATA 
GAGGAGAACA AGAAACAGGG CAAGTACCCA GAAGTCCACA TTCCCCTCGA GAATCTACAG 
CAGTTTCCTG CCTTCAATTC TTATACCTGC TCCTCTGATT CTGCTTTGGG TCCCACCAGC 
ACCGAGGGCC ATGGGGCCCT CTCCATTTCT CCTCTCAGCA GAAGTCCAGG TTCCCATGCA 
GACTTTCTGC TGACCCCTCT GTCACCCACT GCCAGTCAGG GCAGTCCCTC ATTCTGCGTT 
GGGAGTCTGG AAGAAGACTC TCCCTTCCCT TCCTTTGCCC AGATGCTGAG GGTTGGAAAA 
GCAAAAGCAG ATGTGTGGCC CAAAACTGCT CCAAAGAAAG ATGAGAACAG CTTAGTTCCT 
CCTGCCCCTG TGGACAGCGA CGGGGAGAGT GATAATTCAG ACCGTGTTCC TGTGCCCAGT 
TTTCAAAATT CCTTCAGCCA AGCTATTGAA GCAGCCTTCA TGAAACTGGA CACACCAGCT 
ACTTCAGATC CCCTCTCTGA AGAGAAAGGA GGAAAGAAAA GAAAAAAACA GAAACAGAAG 
CTCCTGTTCA GCACCTCAGT CGTCCACACC AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47004
Clone ID Related Accession (Same CDS sequence) NM_001330474.1 , NM_001330474.2
Accession Version NM_001330474.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-25
Organism Homo sapiens(human)
Product RING finger protein 10 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA956437.1, AB027196.1, BU184035.1, HY376488.1 and BQ015862.1. On Aug 25, 2016 this sequence version replaced XM_006719717.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is longer, but has the same N- and C-termini compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.201022.1, SRR1803613.265303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGACTT TCTGCTGACC CCTCTGTCAC CCACTGCCAG TCAGGGCAGT 
CCCTCATTCT GCGTTGGGAG TCTGGAAGAA GACTCTCCCT TCCCTTCCTT TGCCCAGATG 
CTGAGGGTTG GAAAAGCAAA AGCAGATGTG TGGCCCAAAA CTGCTCCAAA GAAAGATGAG 
AACAGCTTAG TTCCTCCTGC CCCTGTGGAC AGCGACGGGG AGAGTGATAA TTCAGACCGT 
GTTCCTGTGC CCAGTTTTCA AAATTCCTTC AGCCAAGCTA TTGAAGCAGC CTTCATGAAA 
CTGGACACAC CAGCTACTTC AGATCCCCTC TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA 
AAACAGAAAC AGAAGCTCCT GTTCAGCACC TCAGTCGTCC ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317403.1
CDS483..2933
Translation

Target ORF information:

RefSeq Version NM_001330474.1
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001330474.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGACTT TCTGCTGACC CCTCTGTCAC CCACTGCCAG TCAGGGCAGT 
CCCTCATTCT GCGTTGGGAG TCTGGAAGAA GACTCTCCCT TCCCTTCCTT TGCCCAGATG 
CTGAGGGTTG GAAAAGCAAA AGCAGATGTG TGGCCCAAAA CTGCTCCAAA GAAAGATGAG 
AACAGCTTAG TTCCTCCTGC CCCTGTGGAC AGCGACGGGG AGAGTGATAA TTCAGACCGT 
GTTCCTGTGC CCAGTTTTCA AAATTCCTTC AGCCAAGCTA TTGAAGCAGC CTTCATGAAA 
CTGGACACAC CAGCTACTTC AGATCCCCTC TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA 
AAACAGAAAC AGAAGCTCCT GTTCAGCACC TCAGTCGTCC ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47004
Clone ID Related Accession (Same CDS sequence) NM_001330474.1 , NM_001330474.2
Accession Version NM_001330474.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2451bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-27
Organism Homo sapiens(human)
Product RING finger protein 10 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA956437.1, AB027196.1, BU184035.1, HY376488.1 and BQ015862.1. On May 31, 2019 this sequence version replaced NM_001330474.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is longer, but has the same N- and C-termini compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.141311.1, SRR1803611.201022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGACTT TCTGCTGACC CCTCTGTCAC CCACTGCCAG TCAGGGCAGT 
CCCTCATTCT GCGTTGGGAG TCTGGAAGAA GACTCTCCCT TCCCTTCCTT TGCCCAGATG 
CTGAGGGTTG GAAAAGCAAA AGCAGATGTG TGGCCCAAAA CTGCTCCAAA GAAAGATGAG 
AACAGCTTAG TTCCTCCTGC CCCTGTGGAC AGCGACGGGG AGAGTGATAA TTCAGACCGT 
GTTCCTGTGC CCAGTTTTCA AAATTCCTTC AGCCAAGCTA TTGAAGCAGC CTTCATGAAA 
CTGGACACAC CAGCTACTTC AGATCCCCTC TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA 
AAACAGAAAC AGAAGCTCCT GTTCAGCACC TCAGTCGTCC ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317403.1
CDS457..2907
Translation

Target ORF information:

RefSeq Version NM_001330474.2
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001330474.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGACTT TCTGCTGACC CCTCTGTCAC CCACTGCCAG TCAGGGCAGT 
CCCTCATTCT GCGTTGGGAG TCTGGAAGAA GACTCTCCCT TCCCTTCCTT TGCCCAGATG 
CTGAGGGTTG GAAAAGCAAA AGCAGATGTG TGGCCCAAAA CTGCTCCAAA GAAAGATGAG 
AACAGCTTAG TTCCTCCTGC CCCTGTGGAC AGCGACGGGG AGAGTGATAA TTCAGACCGT 
GTTCCTGTGC CCAGTTTTCA AAATTCCTTC AGCCAAGCTA TTGAAGCAGC CTTCATGAAA 
CTGGACACAC CAGCTACTTC AGATCCCCTC TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA 
AAACAGAAAC AGAAGCTCCT GTTCAGCACC TCAGTCGTCC ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu47005
Clone ID Related Accession (Same CDS sequence) XM_005254013.2
Accession Version XM_005254013.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product RING finger protein 10 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGATGGATG GAAAGAACTC CAGTGGATCC AAGCGTTATA ATCGCAAACG TGAACTTTCC 
TACCCCAAAA ATGAAAGTTT TAACAACCAG TCCCGTCGCT CCAGTTCACA GAAAAGCAAG 
ACTTTTAACA AGATGCCTCC TCAAAGGGGC GGCGGCAGCA GCAAACTCTT TAGCTCTTCT 
TTTAATGGTG GAAGACGAGA TGAGGTAGCA GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC 
CAGTTCTCTG GTCCTAAGAA GATCAACCTG AACCACTTGT TGAATTTCAC TTTTGAACCC 
CGTGGCCAGA CGGGTCACTT TGAAGGCAGT GGACATGGTA GCTGGGGAAA GAGGAACAAG 
TGGGGACATA AGCCTTTTAA CAAGGAACTC TTTTTACAGG CCAACTGCCA ATTTGTGGTG 
TCTGAAGACC AAGACTACAC AGCTCATTTT GCTGATCCTG ATACATTAGT TAACTGGGAC 
TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT 
CCACCTACTG CAGCCAAGAT AACCCGTTGT GGACACATCT TCTGCTGGGC ATGCATCCTG 
CACTATCTTT CACTGAGTGA GAAGACGTGG AGTAAATGTC CCATCTGTTA CAGTTCTGTG 
CATAAGAAGG ATCTCAAGAG TGTTGTTGCC ACAGAGTCAC ATCAGTATGT TGTTGGTGAT 
ACCATTACGA TGCAGCTGAT GAAGAGGGAG AAAGGGGTGT TGGTGGCTTT GCCCAAATCC 
AAATGGATGA ATGTAGACCA TCCCATTCAT CTAGGAGATG AACAGCACAG CCAGTACTCC 
AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG CTGCACCGGG TAGTTCTGGA GGAGAAAGTA 
GCACTAGAGC AGCAGCTGGC AGAGGAGAAG CACACTCCCG AGTCCTGCTT TATTGAGGCA 
GCTATCCAGG AGCTCAAGAC TCGGGAAGAG GCTCTGTCGG GATTGGCCGG AAGCAGAAGG 
GAGGTCACTG GTGTTGTGGC TGCTCTGGAA CAACTGGTGC TGATGGCTCC CTTGGCGAAG 
GAGTCTGTTT TTCAACCCAG GAAGAGTTTG CTGCAACAGG GTGTGCTGGA GTATCTGTCT 
GCCTTCGATG AAGAAACCAC GGAAGTTTGT TCTCTGGACA CTCCTTCTAG ACCTCTTGCT 
CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG TCTGAACCAG AGCCTGAGGG GTTGCCAGAG 
GCCTGTGATG ACTTGGAGTT AGCAGATGAC AATCTTAAAG AGGGGACCAT TTGCACTGAG 
TCCAGCCAGC AGGAACCCAT CACCAAGTCA GGCTTCACAC GCCTCAGCAG CTCTCCTTGT 
TACTACTTTT ACCAAGCGGA AGATGGACAG CATATGTTCC TGCACCCTGT GAATGTGCGC 
TGCCTCGTGC GGGAGTACGG CAGCCTGGAG AGGAGCCCCG AGAAGATCTC AGCAACTGTG 
GTGGAGATTG CTGGCTACTC CATGTCTGAG GATGTTCGAC AGCGTCACAG ATATCTCTCT 
CACTTGCCAC TCACCTGTGA GTTCAGCATC TGTGAACTGG CTTTGCAACC TCCTGTGGTC 
TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT GACATTGAGA AGAGGAAACG TCAGCGCCAA 
AAGAAGGCTC GGGAGGAACG CCGCCGAGAG CGCAGGATTG AGATAGAGGA GAACAAGAAA 
CAGGGCAAGT ACCCAGAAGT CCACATTCCC CTCGAGAATC TACAGCAGTT TCCTGCCTTC 
AATTCTTATA CCTGCTCCTC TGATTCTGCT TTGGGTCCCA CCAGCACCGA GGGCCATGGG 
GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT CCAGGTTCCC ATGCAGACTT TCTGCTGACC 
CCTCTGTCAC CCACTGCCAG TCAGGGCAGT CCCTCATTCT GCGTTGGGAG TCTGGAAGAA 
GACTCTCCCT TCCCTTCCTT TGCCCAGATG CTGAGGGTTG GAAAAGCAAA AGCAGATGTG 
TGGCCCAAAA CTGCTCCAAA GAAAGATGAG AACAGCTTAG TTCCTCCTGC CCCTGTGGAC 
AGCGACGGGG AGAGTGATAA TTCAGACCGT GTTCCTGTGC CCAGTTTTCA AAATTCCTTC 
AGCCAAGCTA TTGAAGCAGC CTTCATGAAA CTGGACACAC CAGCTACTTC AGATCCCCTC 
TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA AAACAGAAAC AGAAGCTCCT GTTCAGCACC 
TCAGTCGTCC ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005254070.1
CDS711..3011
Translation

Target ORF information:

RefSeq Version XM_005254013.2
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005254013.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGATGGATG GAAAGAACTC CAGTGGATCC AAGCGTTATA ATCGCAAACG TGAACTTTCC 
TACCCCAAAA ATGAAAGTTT TAACAACCAG TCCCGTCGCT CCAGTTCACA GAAAAGCAAG 
ACTTTTAACA AGATGCCTCC TCAAAGGGGC GGCGGCAGCA GCAAACTCTT TAGCTCTTCT 
TTTAATGGTG GAAGACGAGA TGAGGTAGCA GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC 
CAGTTCTCTG GTCCTAAGAA GATCAACCTG AACCACTTGT TGAATTTCAC TTTTGAACCC 
CGTGGCCAGA CGGGTCACTT TGAAGGCAGT GGACATGGTA GCTGGGGAAA GAGGAACAAG 
TGGGGACATA AGCCTTTTAA CAAGGAACTC TTTTTACAGG CCAACTGCCA ATTTGTGGTG 
TCTGAAGACC AAGACTACAC AGCTCATTTT GCTGATCCTG ATACATTAGT TAACTGGGAC 
TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT 
CCACCTACTG CAGCCAAGAT AACCCGTTGT GGACACATCT TCTGCTGGGC ATGCATCCTG 
CACTATCTTT CACTGAGTGA GAAGACGTGG AGTAAATGTC CCATCTGTTA CAGTTCTGTG 
CATAAGAAGG ATCTCAAGAG TGTTGTTGCC ACAGAGTCAC ATCAGTATGT TGTTGGTGAT 
ACCATTACGA TGCAGCTGAT GAAGAGGGAG AAAGGGGTGT TGGTGGCTTT GCCCAAATCC 
AAATGGATGA ATGTAGACCA TCCCATTCAT CTAGGAGATG AACAGCACAG CCAGTACTCC 
AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG CTGCACCGGG TAGTTCTGGA GGAGAAAGTA 
GCACTAGAGC AGCAGCTGGC AGAGGAGAAG CACACTCCCG AGTCCTGCTT TATTGAGGCA 
GCTATCCAGG AGCTCAAGAC TCGGGAAGAG GCTCTGTCGG GATTGGCCGG AAGCAGAAGG 
GAGGTCACTG GTGTTGTGGC TGCTCTGGAA CAACTGGTGC TGATGGCTCC CTTGGCGAAG 
GAGTCTGTTT TTCAACCCAG GAAGAGTTTG CTGCAACAGG GTGTGCTGGA GTATCTGTCT 
GCCTTCGATG AAGAAACCAC GGAAGTTTGT TCTCTGGACA CTCCTTCTAG ACCTCTTGCT 
CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG TCTGAACCAG AGCCTGAGGG GTTGCCAGAG 
GCCTGTGATG ACTTGGAGTT AGCAGATGAC AATCTTAAAG AGGGGACCAT TTGCACTGAG 
TCCAGCCAGC AGGAACCCAT CACCAAGTCA GGCTTCACAC GCCTCAGCAG CTCTCCTTGT 
TACTACTTTT ACCAAGCGGA AGATGGACAG CATATGTTCC TGCACCCTGT GAATGTGCGC 
TGCCTCGTGC GGGAGTACGG CAGCCTGGAG AGGAGCCCCG AGAAGATCTC AGCAACTGTG 
GTGGAGATTG CTGGCTACTC CATGTCTGAG GATGTTCGAC AGCGTCACAG ATATCTCTCT 
CACTTGCCAC TCACCTGTGA GTTCAGCATC TGTGAACTGG CTTTGCAACC TCCTGTGGTC 
TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT GACATTGAGA AGAGGAAACG TCAGCGCCAA 
AAGAAGGCTC GGGAGGAACG CCGCCGAGAG CGCAGGATTG AGATAGAGGA GAACAAGAAA 
CAGGGCAAGT ACCCAGAAGT CCACATTCCC CTCGAGAATC TACAGCAGTT TCCTGCCTTC 
AATTCTTATA CCTGCTCCTC TGATTCTGCT TTGGGTCCCA CCAGCACCGA GGGCCATGGG 
GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT CCAGGTTCCC ATGCAGACTT TCTGCTGACC 
CCTCTGTCAC CCACTGCCAG TCAGGGCAGT CCCTCATTCT GCGTTGGGAG TCTGGAAGAA 
GACTCTCCCT TCCCTTCCTT TGCCCAGATG CTGAGGGTTG GAAAAGCAAA AGCAGATGTG 
TGGCCCAAAA CTGCTCCAAA GAAAGATGAG AACAGCTTAG TTCCTCCTGC CCCTGTGGAC 
AGCGACGGGG AGAGTGATAA TTCAGACCGT GTTCCTGTGC CCAGTTTTCA AAATTCCTTC 
AGCCAAGCTA TTGAAGCAGC CTTCATGAAA CTGGACACAC CAGCTACTTC AGATCCCCTC 
TCTGAAGAGA AAGGAGGAAA GAAAAGAAAA AAACAGAAAC AGAAGCTCCT GTTCAGCACC 
TCAGTCGTCC ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84364
Clone ID Related Accession (Same CDS sequence) XM_017020282.1
Accession Version XM_017020282.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product RING finger protein 10 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGATGGATG GAAAGAACTC CAGTGGATCC AAGCGTTATA ATCGCAAACG TGAACTTTCC 
TACCCCAAAA ATGAAAGTTT TAACAACCAG TCCCGTCGCT CCAGTTCACA GAAAAGCAAG 
ACTTTTAACA AGATGCCTCC TCAAAGGGGC GGCGGCAGCA GCAAACTCTT TAGCTCTTCT 
TTTAATGGTG GAAGACGAGA TGAGGTAGCA GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC 
CAGTTCTCTG GTCCTAAGAA GATCAACCTG AACCACTTGT TGAATTTCAC TTTTGAACCC 
CGTGGCCAGA CGGGTCACTT TGAAGGCAGT GGACATGGTA GCTGGGGAAA GAGGAACAAG 
TGGGGACATA AGCCTTTTAA CAAGGAACTC TTTTTACAGG CCAACTGCCA ATTTGTGGTG 
TCTGAAGACC AAGACTACAC AGCTCATTTT GCTGATCCTG ATACATTAGT TAACTGGGAC 
TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT 
CCACCTACTG CAGCCAAGAT AACCCGTTGT GGACACATCT TCTGCTGGGC ATGCATCCTG 
CACTATCTTT CACTGAGTGA GAAGACGTGG AGTAAATGTC CCATCTGTTA CAGTTCTGTG 
CATAAGAAGG ATCTCAAGAG TGTTGTTGCC ACAGAGTCAC ATCAGTATGT TGTTGGTGAT 
ACCATTACGA TGCAGCTGAT GAAGAGGGAG AAAGGGGTGT TGGTGGCTTT GCCCAAATCC 
AAATGGATGA ATGTAGACCA TCCCATTCAT CTAGGAGATG AACAGCACAG CCAGTACTCC 
AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG CTGCACCGGG TAGTTCTGGA GGAGAAAGTA 
GCACTAGAGC AGCAGCTGGC AGAGGAGAAG CACACTCCCG AGTCCTGCTT TATTGAGGCA 
GCTATCCAGG AGCTCAAGAC TCGGGAAGAG GCTCTGTCGG GATTGGCCGG AAGCAGAAGG 
GAGGTCACTG GTGTTGTGGC TGCTCTGGAA CAACTGGTGC TGATGGCTCC CTTGGCGAAG 
GAGTCTGTTT TTCAACCCAG GAAGGGTGTG CTGGAGTATC TGTCTGCCTT CGATGAAGAA 
ACCACGGAAG TTTGTTCTCT GGACACTCCT TCTAGACCTC TTGCTCTCCC TCTGGTAGAA 
GAGGAGGAAG CAGTGTCTGA ACCAGAGCCT GAGGGGTTGC CAGAGGCCTG TGATGACTTG 
GAGTTAGCAG ATGACAATCT TAAAGAGGGG ACCATTTGCA CTGAGTCCAG CCAGCAGGAA 
CCCATCACCA AGTCAGGCTT CACACGCCTC AGCAGCTCTC CTTGTTACTA CTTTTACCAA 
GCGGAAGATG GACAGCATAT GTTCCTGCAC CCTGTGAATG TGCGCTGCCT CGTGCGGGAG 
TACGGCAGCC TGGAGAGGAG CCCCGAGAAG ATCTCAGCAA CTGTGGTGGA GATTGCTGGC 
TACTCCATGT CTGAGGATGT TCGACAGCGT CACAGATATC TCTCTCACTT GCCACTCACC 
TGTGAGTTCA GCATCTGTGA ACTGGCTTTG CAACCTCCTG TGGTCTCTAA GGAAACCCTA 
GAGATGTTCT CAGATGACAT TGAGAAGAGG AAACGTCAGC GCCAAAAGAA GGCTCGGGAG 
GAACGCCGCC GAGAGCGCAG GATTGAGATA GAGGAGAACA AGAAACAGGG CAAGTACCCA 
GAAGTCCACA TTCCCCTCGA GAATCTACAG CAGTTTCCTG CCTTCAATTC TTATACCTGC 
TCCTCTGATT CTGCTTTGGG TCCCACCAGC ACCGAGGGCC ATGGGGCCCT CTCCATTTCT 
CCTCTCAGCA GAAGTCCAGG TTCCCATGCA GACTTTCTGC TGACCCCTCT GTCACCCACT 
GCCAGTCAGG GCAGTCCCTC ATTCTGCGTT GGGAGTCTGG AAGAAGACTC TCCCTTCCCT 
TCCTTTGCCC AGATGCTGAG GGTTGGAAAA GCAAAAGCAG ATGTGTGGCC CAAAACTGCT 
CCAAAGAAAG ATGAGAACAG CTTAGTTCCT CCTGCCCCTG TGGACAGCGA CGGGGAGAGT 
GATAATTCAG ACCGTGTTCC TGTGCCCAGT TTTCAAAATT CCTTCAGCCA AGCTATTGAA 
GCAGCCTTCA TGAAACTGGA CACACCAGCT ACTTCAGATC CCCTCTCTGA AGAGAAAGGA 
GGAAAGAAAA GAAAAAAACA GAAACAGAAG CTCCTGTTCA GCACCTCAGT CGTCCACACC 
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875771.1
CDS711..2996
Translation

Target ORF information:

RefSeq Version XM_017020282.1
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020282.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGATGGATG GAAAGAACTC CAGTGGATCC AAGCGTTATA ATCGCAAACG TGAACTTTCC 
TACCCCAAAA ATGAAAGTTT TAACAACCAG TCCCGTCGCT CCAGTTCACA GAAAAGCAAG 
ACTTTTAACA AGATGCCTCC TCAAAGGGGC GGCGGCAGCA GCAAACTCTT TAGCTCTTCT 
TTTAATGGTG GAAGACGAGA TGAGGTAGCA GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC 
CAGTTCTCTG GTCCTAAGAA GATCAACCTG AACCACTTGT TGAATTTCAC TTTTGAACCC 
CGTGGCCAGA CGGGTCACTT TGAAGGCAGT GGACATGGTA GCTGGGGAAA GAGGAACAAG 
TGGGGACATA AGCCTTTTAA CAAGGAACTC TTTTTACAGG CCAACTGCCA ATTTGTGGTG 
TCTGAAGACC AAGACTACAC AGCTCATTTT GCTGATCCTG ATACATTAGT TAACTGGGAC 
TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT 
CCACCTACTG CAGCCAAGAT AACCCGTTGT GGACACATCT TCTGCTGGGC ATGCATCCTG 
CACTATCTTT CACTGAGTGA GAAGACGTGG AGTAAATGTC CCATCTGTTA CAGTTCTGTG 
CATAAGAAGG ATCTCAAGAG TGTTGTTGCC ACAGAGTCAC ATCAGTATGT TGTTGGTGAT 
ACCATTACGA TGCAGCTGAT GAAGAGGGAG AAAGGGGTGT TGGTGGCTTT GCCCAAATCC 
AAATGGATGA ATGTAGACCA TCCCATTCAT CTAGGAGATG AACAGCACAG CCAGTACTCC 
AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG CTGCACCGGG TAGTTCTGGA GGAGAAAGTA 
GCACTAGAGC AGCAGCTGGC AGAGGAGAAG CACACTCCCG AGTCCTGCTT TATTGAGGCA 
GCTATCCAGG AGCTCAAGAC TCGGGAAGAG GCTCTGTCGG GATTGGCCGG AAGCAGAAGG 
GAGGTCACTG GTGTTGTGGC TGCTCTGGAA CAACTGGTGC TGATGGCTCC CTTGGCGAAG 
GAGTCTGTTT TTCAACCCAG GAAGGGTGTG CTGGAGTATC TGTCTGCCTT CGATGAAGAA 
ACCACGGAAG TTTGTTCTCT GGACACTCCT TCTAGACCTC TTGCTCTCCC TCTGGTAGAA 
GAGGAGGAAG CAGTGTCTGA ACCAGAGCCT GAGGGGTTGC CAGAGGCCTG TGATGACTTG 
GAGTTAGCAG ATGACAATCT TAAAGAGGGG ACCATTTGCA CTGAGTCCAG CCAGCAGGAA 
CCCATCACCA AGTCAGGCTT CACACGCCTC AGCAGCTCTC CTTGTTACTA CTTTTACCAA 
GCGGAAGATG GACAGCATAT GTTCCTGCAC CCTGTGAATG TGCGCTGCCT CGTGCGGGAG 
TACGGCAGCC TGGAGAGGAG CCCCGAGAAG ATCTCAGCAA CTGTGGTGGA GATTGCTGGC 
TACTCCATGT CTGAGGATGT TCGACAGCGT CACAGATATC TCTCTCACTT GCCACTCACC 
TGTGAGTTCA GCATCTGTGA ACTGGCTTTG CAACCTCCTG TGGTCTCTAA GGAAACCCTA 
GAGATGTTCT CAGATGACAT TGAGAAGAGG AAACGTCAGC GCCAAAAGAA GGCTCGGGAG 
GAACGCCGCC GAGAGCGCAG GATTGAGATA GAGGAGAACA AGAAACAGGG CAAGTACCCA 
GAAGTCCACA TTCCCCTCGA GAATCTACAG CAGTTTCCTG CCTTCAATTC TTATACCTGC 
TCCTCTGATT CTGCTTTGGG TCCCACCAGC ACCGAGGGCC ATGGGGCCCT CTCCATTTCT 
CCTCTCAGCA GAAGTCCAGG TTCCCATGCA GACTTTCTGC TGACCCCTCT GTCACCCACT 
GCCAGTCAGG GCAGTCCCTC ATTCTGCGTT GGGAGTCTGG AAGAAGACTC TCCCTTCCCT 
TCCTTTGCCC AGATGCTGAG GGTTGGAAAA GCAAAAGCAG ATGTGTGGCC CAAAACTGCT 
CCAAAGAAAG ATGAGAACAG CTTAGTTCCT CCTGCCCCTG TGGACAGCGA CGGGGAGAGT 
GATAATTCAG ACCGTGTTCC TGTGCCCAGT TTTCAAAATT CCTTCAGCCA AGCTATTGAA 
GCAGCCTTCA TGAAACTGGA CACACCAGCT ACTTCAGATC CCCTCTCTGA AGAGAAAGGA 
GGAAAGAAAA GAAAAAAACA GAAACAGAAG CTCCTGTTCA GCACCTCAGT CGTCCACACC 
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84365
Clone ID Related Accession (Same CDS sequence) XM_017020283.1 , XM_017020283.2
Accession Version XM_017020283.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product RING finger protein 10 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGGTCT CTGGTTCCCT TTCAGACTTT CTGCTGACCC CTCTGTCACC 
CACTGCCAGT CAGGGCAGTC CCTCATTCTG CGTTGGGAGT CTGGAAGAAG ACTCTCCCTT 
CCCTTCCTTT GCCCAGGAAT TTTACTGGCC TGTGGTTGGA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875772.1
CDS504..2708
Translation

Target ORF information:

RefSeq Version XM_017020283.1
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020283.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGGTCT CTGGTTCCCT TTCAGACTTT CTGCTGACCC CTCTGTCACC 
CACTGCCAGT CAGGGCAGTC CCTCATTCTG CGTTGGGAGT CTGGAAGAAG ACTCTCCCTT 
CCCTTCCTTT GCCCAGGAAT TTTACTGGCC TGTGGTTGGA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu84365
Clone ID Related Accession (Same CDS sequence) XM_017020283.1 , XM_017020283.2
Accession Version XM_017020283.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product RING finger protein 10 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGGTCT CTGGTTCCCT TTCAGACTTT CTGCTGACCC CTCTGTCACC 
CACTGCCAGT CAGGGCAGTC CCTCATTCTG CGTTGGGAGT CTGGAAGAAG ACTCTCCCTT 
CCCTTCCTTT GCCCAGGAAT TTTACTGGCC TGTGGTTGGA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016875772.1
CDS460..2664
Translation

Target ORF information:

RefSeq Version XM_017020283.2
Organism Homo sapiens(human)
Definition Homo sapiens ring finger protein 10 (RNF10), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017020283.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCCGCTGA GCTCCCCCAA CGCCGCCGCC ACCGCCTCCG ACATGGACAA GAACAGCGGC 
TCCAACAGCT CCTCCGCCTC TTCGGGCAGC AGCAAAGGGC AACAGCCGCC CCGCTCCGCC 
TCGGCGGGGC CAGCCGGCGA GTCTAAACCC AAGAGCGATG GAAAGAACTC CAGTGGATCC 
AAGCGTTATA ATCGCAAACG TGAACTTTCC TACCCCAAAA ATGAAAGTTT TAACAACCAG 
TCCCGTCGCT CCAGTTCACA GAAAAGCAAG ACTTTTAACA AGATGCCTCC TCAAAGGGGC 
GGCGGCAGCA GCAAACTCTT TAGCTCTTCT TTTAATGGTG GAAGACGAGA TGAGGTAGCA 
GAGGCTCAAC GGGCAGAGTT TAGCCCTGCC CAGTTCTCTG GTCCTAAGAA GATCAACCTG 
AACCACTTGT TGAATTTCAC TTTTGAACCC CGTGGCCAGA CGGGTCACTT TGAAGGCAGT 
GGACATGGTA GCTGGGGAAA GAGGAACAAG TGGGGACATA AGCCTTTTAA CAAGGAACTC 
TTTTTACAGG CCAACTGCCA ATTTGTGGTG TCTGAAGACC AAGACTACAC AGCTCATTTT 
GCTGATCCTG ATACATTAGT TAACTGGGAC TTTGTGGAAC AAGTGCGCAT TTGTAGCCAT 
GAAGTGCCAT CTTGCCCAAT ATGCCTCTAT CCACCTACTG CAGCCAAGAT AACCCGTTGT 
GGACACATCT TCTGCTGGGC ATGCATCCTG CACTATCTTT CACTGAGTGA GAAGACGTGG 
AGTAAATGTC CCATCTGTTA CAGTTCTGTG CATAAGAAGG ATCTCAAGAG TGTTGTTGCC 
ACAGAGTCAC ATCAGTATGT TGTTGGTGAT ACCATTACGA TGCAGCTGAT GAAGAGGGAG 
AAAGGGGTGT TGGTGGCTTT GCCCAAATCC AAATGGATGA ATGTAGACCA TCCCATTCAT 
CTAGGAGATG AACAGCACAG CCAGTACTCC AAGTTGCTGC TGGCCTCTAA GGAGCAGGTG 
CTGCACCGGG TAGTTCTGGA GGAGAAAGTA GCACTAGAGC AGCAGCTGGC AGAGGAGAAG 
CACACTCCCG AGTCCTGCTT TATTGAGGCA GCTATCCAGG AGCTCAAGAC TCGGGAAGAG 
GCTCTGTCGG GATTGGCCGG AAGCAGAAGG GAGGTCACTG GTGTTGTGGC TGCTCTGGAA 
CAACTGGTGC TGATGGCTCC CTTGGCGAAG GAGTCTGTTT TTCAACCCAG GAAGAGTTTG 
CTGCAACAGG GTGTGCTGGA GTATCTGTCT GCCTTCGATG AAGAAACCAC GGAAGTTTGT 
TCTCTGGACA CTCCTTCTAG ACCTCTTGCT CTCCCTCTGG TAGAAGAGGA GGAAGCAGTG 
TCTGAACCAG AGCCTGAGGG GTTGCCAGAG GCCTGTGATG ACTTGGAGTT AGCAGATGAC 
AATCTTAAAG AGGGGACCAT TTGCACTGAG TCCAGCCAGC AGGAACCCAT CACCAAGTCA 
GGCTTCACAC GCCTCAGCAG CTCTCCTTGT TACTACTTTT ACCAAGCGGA AGATGGACAG 
CATATGTTCC TGCACCCTGT GAATGTGCGC TGCCTCGTGC GGGAGTACGG CAGCCTGGAG 
AGGAGCCCCG AGAAGATCTC AGCAACTGTG GTGGAGATTG CTGGCTACTC CATGTCTGAG 
GATGTTCGAC AGCGTCACAG ATATCTCTCT CACTTGCCAC TCACCTGTGA GTTCAGCATC 
TGTGAACTGG CTTTGCAACC TCCTGTGGTC TCTAAGGAAA CCCTAGAGAT GTTCTCAGAT 
GACATTGAGA AGAGGAAACG TCAGCGCCAA AAGAAGGCTC GGGAGGAACG CCGCCGAGAG 
CGCAGGATTG AGATAGAGGA GAACAAGAAA CAGGGCAAGT ACCCAGAAGT CCACATTCCC 
CTCGAGAATC TACAGCAGTT TCCTGCCTTC AATTCTTATA CCTGCTCCTC TGATTCTGCT 
TTGGGTCCCA CCAGCACCGA GGGCCATGGG GCCCTCTCCA TTTCTCCTCT CAGCAGAAGT 
CCAGGTTCCC ATGCAGGTCT CTGGTTCCCT TTCAGACTTT CTGCTGACCC CTCTGTCACC 
CACTGCCAGT CAGGGCAGTC CCTCATTCTG CGTTGGGAGT CTGGAAGAAG ACTCTCCCTT 
CCCTTCCTTT GCCCAGGAAT TTTACTGGCC TGTGGTTGGA TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Whole exome sequencing identifies variation in CYB5A and RNF10 associated with adiposity and type 2 diabetes.
Obesity (Silver Spring, Md.)22(4)984-8(2014 Apr)
Huang K,Nair AK,Muller YL,Piaggi P,Bian L,Del Rosario M,Knowler WC,Kobes S,Hanson RL,Bogardus C,Baier LJ


book

Protein microarray characterization of the S-nitrosoproteome.
Molecular & cellular proteomics : MCP13(1)63-72(2014 Jan)
Lee YI,Giovinazzo D,Kang HC,Lee Y,Jeong JS,Doulias PT,Xie Z,Hu J,Ghasemi M,Ischiropoulos H,Qian J,Zhu H,Blackshaw S,Dawson VL,Dawson TM


book

New susceptibility loci associated with kidney disease in type 1 diabetes.
PLoS genetics8(9)e1002921(2012 Sep)
Sandholm N,Salem RM,McKnight AJ,Brennan EP,Forsblom C,Isakova T,McKay GJ,Williams WW,Sadlier DM,M?kinen VP,Swan EJ,Palmer C,Boright AP,Ahlqvist E,Deshmukh HA,Keller BJ,Huang H,Ahola AJ,Fagerholm E,Gordin D,Harjutsalo V,He B,Heikkil? O,Hietala K,Kyt? J,Lahermo P,Lehto M,Lithovius R,Osterholm AM,Parkkonen M,Pitk?niemi J,Roseng?rd-B?rlund M,Saraheimo M,Sarti C,S?derlund J,Soro-Paavonen A,Syreeni A,Thorn LM,Tikkanen H,Tolonen N,Tryggvason K,Tuomilehto J,Wad?n J,Gill GV,Prior S,Guiducci C,Mirel DB,Taylor A,Hosseini SM,,Parving HH,Rossing P,Tarnow L,Ladenvall C,Alhenc-Gelas F,Lefebvre P,Rigalleau V,Roussel R,Tregouet DA,Maestroni A,Maestroni S,Falhammar H,Gu T,M?llsten A,Cimponeriu D,Ioana M,Mota M,Mota E,Serafinceanu C,Stavarachi M,Hanson RL,Nelson RG,Kretzler M,Colhoun HM,Panduru NM,Gu HF,Brismar K,Zerbini G,Hadjadj S,Marre M,Groop L,Lajer M,Bull SB,Waggott D,Paterson AD,Savage DA,Bain SC,Martin F,Hirschhorn JN,Godson C,Florez JC,Groop PH,Maxwell AP


book

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells.
Molecular & cellular proteomics : MCP10(2)M110.001628(2011 Feb)
Lee OH,Kim H,He Q,Baek HJ,Yang D,Chen LY,Liang J,Chae HK,Safari A,Liu D,Songyang Z


book

A novel function of RING finger protein 10 in transcriptional regulation of the myelin-associated glycoprotein gene and myelin formation in Schwann cells.
PloS one3(10)e3464(2008)
Hoshikawa S,Ogata T,Fujiwara S,Nakamura K,Tanaka S