LOC8030472 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8030472
Entrez Gene ID	8030472
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029988196.1	XP_029844056.1	trafficking protein particle complex subunit 8 isoform X1
XM_002401286.1	XP_002401330.1	hypothetical protein
XM_029988197.1	XP_029844057.1	trafficking protein particle complex subunit 8 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8030472 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8030472 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa04155	XM_002401286.1 Latest version!	Ixodes scapularis conserved hypothetical protein, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OIa30113	XM_029988196.1 Latest version!	Ixodes scapularis trafficking protein particle complex subunit 8 (LOC8030472), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OIa30114	XM_029988197.1 Latest version!	Ixodes scapularis trafficking protein particle complex subunit 8 (LOC8030472), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8030472 ( XM_002401286.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002401330.1 Ixodes scapularis conserved hypothetical protein, mRNA.

Price & Availability↑

CloneID	OIa04155
Clone ID Related Accession (Same CDS sequence)	XM_002401286.1
Accession Version	XM_002401286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002668661). COMPLETENESS: incomplete on both ends.

ATGCAGCCAG GCAAGCGAGG CGTCCAGGAG TTCATCCAAG GCGTCTTTGC GCCGATGATC 
ACGGCCCAGT GCAGCCCCGA CGCTGAAGAT GTGTGCCGCA GGAACAATCT TTCCTTCGTC 
GAGCTTCTAC AGCCGTTCTG CCAGGCAGTC GAAGTGACTG TTCGGCCGCC GAACACCATC 
CAGGGCTCGA TTGCCACAAA ACTTCGCCTC TGCGTTCGGG ACCTGAACAA CCCACTGCCC 
CAACTTGCGG CAGCCAAGAA GCTCCTCAAC GACTCGGTGG CCAACGTCCA GCCATCGCTC 
AATGAAAGTG GTGCATCCCT GAGCAGCATT AAAGCTGGCT GTTACAATTT GCAAGTGTCG 
AGTAACTACG GCCAAGATGC TCCTGAACTG CAAGTGAGAC GTCTGGGCGA CCTGGCCTTC 
CTCTTCCAAC TGTACGAGTT TGCCTACCAG GCCTACCACA GTGCCAAGAG GGACTTCAGC 
AGCGACAGTG CTTGGCTTCA CTTTGCCGGC GCACAGGAAA TGGCTGCCCT GGCGGTGTTT 
ATGGCAGGCG TGAATGCCCA GCGACCTTTC CCATTACGTT ACATGGACTC GGCAATTGAC 
CTCTACCTCA ACACCTGCAA ACTTCCCCAG CTGGCAACGA GGGCAGCCCT GCTGAGCACA 
GAGTGCCTTC GCCACCTGGG ACAGCACTCA AGCGTAGCGG CCCAGCTGAT CCGGCTGACG 
AGCGAGGACT CTGACCTGCG CAGTGCGCTT CTCCTCGAGC AGGCGGCCTA CTGCTTCCTT 
CAAGCCGGCA CGGCACCCTC GGTGCGCAAG TATGCCTTCC ACGTGGTGCT AGCCGGACAC 
CGCTACAGCA AGGCAGGGCA CCGCAAGCAC TCCCTTCGGG CCTACCAGGA GGCGCTGCAG 
GTGTACCAGG GCCGCAACTG GAGTCTGGCA GAGGACCACA TCCACTTCAC TATTGGCCGG 
CAGTCGACAT ACCTCAAGCT GCTTCAGGGG GCCCACGAGG CATTCCACTG CCTGCTGGCC 
AAGCACAGCG AGCAGTCACC CACCCAGCAG CTGCTCTTCC TCAGGGAGTA CCTCCTGGTG 
CTCAAGATGT TGCACGAGAA CGACCTGGCA GCAGCCACGG TGCTTCTGCC GGTGCCAGAG 
GTGCACACGA GCTCCACGAG GGTCTACCTG GGACCCGTGG CTGATGAGGG TGCCCTCTGG 
GGGGACCTGG GCACCTGGCT AAAGCTGGAG GAGTGTGCCG CTGCAGTGGC CAACGACGGC 
ACGCTCCCGG CCACCTTTCG TCCCCAGCTG AGTTTGCTCA GCGACGACAC CGACAATGGG 
GCCAGGCCTC TCACAGTCGT CGGGGAACCG GTGACAGTTG AACTAGAACT GCGCAATCCG 
CTCCAAATCG CTCTCCAGCT AAGCCAGGTG CATCTGCTTT GGAGCTTTCT TCCCTCAGAC 
AGGGAGGACC TTATTAGCAA TGACAGAGCT GATGCTCAGG CAAGTGACGT GCCCTTACTG 
GGACTTGCTT CTTTGACGTC TACACTCATC AATACTGGCA TGCTGGACGA AGTTCTCCTC 
GAGTCGGAAC AGAAGCAAAG GGTGCGTTTG ACCTTGGTTC CCCACTGCAC GGGCGACCTC 
CACGTAACCG GGTTTGCCTA TCGGGTTGGG CTCTCGCCCA CCCAACTGGA TGCAACCTCG 
GACCTCGGAC CTCTGCCCAG CCCCGTGAGC GGACGCCAGT TGTTGTCGGT CCAGGGTCCG 
AAGCTGAAAA ACCTCAAGAC TGCCAAAAAC GGACAGTTCT ACTCTCCCGA CTGGAGGCTG 
CATCCGACGG TCGTCGCTGC CATGCCGCAG CTTACGGTCA GCTTCTCCAA TGTTCCCCGG 
CAACTCCTCT GTGGCGAAGT GCAGCGCACC GTTCTACACG TTTCCAATGC GCCGAACCTC 
CCTGAACTCT CATCACTTCT CATCGCTTCA CCGACCCCCC AACTCTTCTG CCTCGGCTTC 
AACGATCCCG ACGACGAAGG CGCTCTGGGT TCGGCGGACG AAGGCATTGC GTACCGTGAA 
AAGCCCCTGC AGTCAAGCAG GGCTGCGCCA GTTTTAGCAC AGCGACCCAC GGCAAACTTC 
GTCTGCAGTC TCCCAGAGGC TGTCTGCAGG ATCCCTGGTG GTGGAGATGC CTCAGTGCCC 
CTGTGGATTC GGGGACCTGA CGTAGTGGGA CGTCACACTC TCAGACTGCT CTTTTACTAC 
GAGAGTGCCC AGAAGAGTAG CAGGCTCACG CACCGTGTTC TCTACCACAA CATCCACTTC 
TCAACGCTTC CCTCGCTGGC TCTGCAGGCC TCAGCTCGAC AGAGCGTGTC AGCAGTCGGG 
GCAGGTTCTC CCAACGGACT TCTAGTTTCC CTTCAGGCCA AAAACTTGAG TGAGGCTGCC 
ATCGAGCGAG ATGGTCAGAT TAAAGTGGTC ATGGGACAGC AGCATGTGAC TTTGAAGGAC 
TTCCATACGC TCTTCTCGTC TGTGCCTGGA CAAAAGGTGA GGTGTTCCCT TGTGAGCCTC 
GTAGTTCACA ACTCGAGCTT TAGGACCGTC ACAGTAAGCA TCGAGCCGGC CGTCAGAACA 
CCACCGGCGG TCGTCCTGTC CAGTCCCGTG ACCACCCCAG AGCTAGTGGC CACCGACGTG 
GGCGGTTTCA GCTGGGTAAC CGGAATCCGG CGCCACTACC CGATGAAGTC GCGCGAGACG 
GTCACCTTTC CGTTGACGGC CGCATTCTCC ATGGCCGGGA CGTACAATGT GGCCGCCCTT 
GAAGTGAGCG TCCTCACGGA CGACGGCCGC CTCTCCCACG AGCCACTGTG TCCGAGCCTC 
GTGGTGGTGC AGCAGCACCG GGCACCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002401330.1
CDS	1..2850
Translation	MQPGKRGVQEFIQGVFAPMITAQCSPDAEDVCRRNNLSFVELLQPFCQAVEVTVRPPNTIQGSIATKLRLCVRDLNNPLPQLAAAKKLLNDSVANVQPSLNESGASLSSIKAGCYNLQVSSNYGQDAPELQVRRLGDLAFLFQLYEFAYQAYHSAKRDFSSDSAWLHFAGAQEMAALAVFMAGVNAQRPFPLRYMDSAIDLYLNTCKLPQLATRAALLSTECLRHLGQHSSVAAQLIRLTSEDSDLRSALLLEQAAYCFLQAGTAPSVRKYAFHVVLAGHRYSKAGHRKHSLRAYQEALQVYQGRNWSLAEDHIHFTIGRQSTYLKLLQGAHEAFHCLLAKHSEQSPTQQLLFLREYLLVLKMLHENDLAAATVLLPVPEVHTSSTRVYLGPVADEGALWGDLGTWLKLEECAAAVANDGTLPATFRPQLSLLSDDTDNGARPLTVVGEPVTVELELRNPLQIALQLSQVHLLWSFLPSDREDLISNDRADAQASDVPLLGLASLTSTLINTGMLDEVLLESEQKQRVRLTLVPHCTGDLHVTGFAYRVGLSPTQLDATSDLGPLPSPVSGRQLLSVQGPKLKNLKTAKNGQFYSPDWRLHPTVVAAMPQLTVSFSNVPRQLLCGEVQRTVLHVSNAPNLPELSSLLIASPTPQLFCLGFNDPDDEGALGSADEGIAYREKPLQSSRAAPVLAQRPTANFVCSLPEAVCRIPGGGDASVPLWIRGPDVVGRHTLRLLFYYESAQKSSRLTHRVLYHNIHFSTLPSLALQASARQSVSAVGAGSPNGLLVSLQAKNLSEAAIERDGQIKVVMGQQHVTLKDFHTLFSSVPGQKVRCSLVSLVVHNSSFRTVTVSIEPAVRTPPAVVLSSPVTTPELVATDVGGFSWVTGIRRHYPMKSRETVTFPLTAAFSMAGTYNVAALEVSVLTDDGRLSHEPLCPSLVVVQQHRAP

Target ORF information:

RefSeq Version	XM_002401286.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis conserved hypothetical protein, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002401286.1

ORF Insert Sequence:

ATGCAGCCAG GCAAGCGAGG CGTCCAGGAG TTCATCCAAG GCGTCTTTGC GCCGATGATC 
ACGGCCCAGT GCAGCCCCGA CGCTGAAGAT GTGTGCCGCA GGAACAATCT TTCCTTCGTC 
GAGCTTCTAC AGCCGTTCTG CCAGGCAGTC GAAGTGACTG TTCGGCCGCC GAACACCATC 
CAGGGCTCGA TTGCCACAAA ACTTCGCCTC TGCGTTCGGG ACCTGAACAA CCCACTGCCC 
CAACTTGCGG CAGCCAAGAA GCTCCTCAAC GACTCGGTGG CCAACGTCCA GCCATCGCTC 
AATGAAAGTG GTGCATCCCT GAGCAGCATT AAAGCTGGCT GTTACAATTT GCAAGTGTCG 
AGTAACTACG GCCAAGATGC TCCTGAACTG CAAGTGAGAC GTCTGGGCGA CCTGGCCTTC 
CTCTTCCAAC TGTACGAGTT TGCCTACCAG GCCTACCACA GTGCCAAGAG GGACTTCAGC 
AGCGACAGTG CTTGGCTTCA CTTTGCCGGC GCACAGGAAA TGGCTGCCCT GGCGGTGTTT 
ATGGCAGGCG TGAATGCCCA GCGACCTTTC CCATTACGTT ACATGGACTC GGCAATTGAC 
CTCTACCTCA ACACCTGCAA ACTTCCCCAG CTGGCAACGA GGGCAGCCCT GCTGAGCACA 
GAGTGCCTTC GCCACCTGGG ACAGCACTCA AGCGTAGCGG CCCAGCTGAT CCGGCTGACG 
AGCGAGGACT CTGACCTGCG CAGTGCGCTT CTCCTCGAGC AGGCGGCCTA CTGCTTCCTT 
CAAGCCGGCA CGGCACCCTC GGTGCGCAAG TATGCCTTCC ACGTGGTGCT AGCCGGACAC 
CGCTACAGCA AGGCAGGGCA CCGCAAGCAC TCCCTTCGGG CCTACCAGGA GGCGCTGCAG 
GTGTACCAGG GCCGCAACTG GAGTCTGGCA GAGGACCACA TCCACTTCAC TATTGGCCGG 
CAGTCGACAT ACCTCAAGCT GCTTCAGGGG GCCCACGAGG CATTCCACTG CCTGCTGGCC 
AAGCACAGCG AGCAGTCACC CACCCAGCAG CTGCTCTTCC TCAGGGAGTA CCTCCTGGTG 
CTCAAGATGT TGCACGAGAA CGACCTGGCA GCAGCCACGG TGCTTCTGCC GGTGCCAGAG 
GTGCACACGA GCTCCACGAG GGTCTACCTG GGACCCGTGG CTGATGAGGG TGCCCTCTGG 
GGGGACCTGG GCACCTGGCT AAAGCTGGAG GAGTGTGCCG CTGCAGTGGC CAACGACGGC 
ACGCTCCCGG CCACCTTTCG TCCCCAGCTG AGTTTGCTCA GCGACGACAC CGACAATGGG 
GCCAGGCCTC TCACAGTCGT CGGGGAACCG GTGACAGTTG AACTAGAACT GCGCAATCCG 
CTCCAAATCG CTCTCCAGCT AAGCCAGGTG CATCTGCTTT GGAGCTTTCT TCCCTCAGAC 
AGGGAGGACC TTATTAGCAA TGACAGAGCT GATGCTCAGG CAAGTGACGT GCCCTTACTG 
GGACTTGCTT CTTTGACGTC TACACTCATC AATACTGGCA TGCTGGACGA AGTTCTCCTC 
GAGTCGGAAC AGAAGCAAAG GGTGCGTTTG ACCTTGGTTC CCCACTGCAC GGGCGACCTC 
CACGTAACCG GGTTTGCCTA TCGGGTTGGG CTCTCGCCCA CCCAACTGGA TGCAACCTCG 
GACCTCGGAC CTCTGCCCAG CCCCGTGAGC GGACGCCAGT TGTTGTCGGT CCAGGGTCCG 
AAGCTGAAAA ACCTCAAGAC TGCCAAAAAC GGACAGTTCT ACTCTCCCGA CTGGAGGCTG 
CATCCGACGG TCGTCGCTGC CATGCCGCAG CTTACGGTCA GCTTCTCCAA TGTTCCCCGG 
CAACTCCTCT GTGGCGAAGT GCAGCGCACC GTTCTACACG TTTCCAATGC GCCGAACCTC 
CCTGAACTCT CATCACTTCT CATCGCTTCA CCGACCCCCC AACTCTTCTG CCTCGGCTTC 
AACGATCCCG ACGACGAAGG CGCTCTGGGT TCGGCGGACG AAGGCATTGC GTACCGTGAA 
AAGCCCCTGC AGTCAAGCAG GGCTGCGCCA GTTTTAGCAC AGCGACCCAC GGCAAACTTC 
GTCTGCAGTC TCCCAGAGGC TGTCTGCAGG ATCCCTGGTG GTGGAGATGC CTCAGTGCCC 
CTGTGGATTC GGGGACCTGA CGTAGTGGGA CGTCACACTC TCAGACTGCT CTTTTACTAC 
GAGAGTGCCC AGAAGAGTAG CAGGCTCACG CACCGTGTTC TCTACCACAA CATCCACTTC 
TCAACGCTTC CCTCGCTGGC TCTGCAGGCC TCAGCTCGAC AGAGCGTGTC AGCAGTCGGG 
GCAGGTTCTC CCAACGGACT TCTAGTTTCC CTTCAGGCCA AAAACTTGAG TGAGGCTGCC 
ATCGAGCGAG ATGGTCAGAT TAAAGTGGTC ATGGGACAGC AGCATGTGAC TTTGAAGGAC 
TTCCATACGC TCTTCTCGTC TGTGCCTGGA CAAAAGGTGA GGTGTTCCCT TGTGAGCCTC 
GTAGTTCACA ACTCGAGCTT TAGGACCGTC ACAGTAAGCA TCGAGCCGGC CGTCAGAACA 
CCACCGGCGG TCGTCCTGTC CAGTCCCGTG ACCACCCCAG AGCTAGTGGC CACCGACGTG 
GGCGGTTTCA GCTGGGTAAC CGGAATCCGG CGCCACTACC CGATGAAGTC GCGCGAGACG 
GTCACCTTTC CGTTGACGGC CGCATTCTCC ATGGCCGGGA CGTACAATGT GGCCGCCCTT 
GAAGTGAGCG TCCTCACGGA CGACGGCCGC CTCTCCCACG AGCCACTGTG TCCGAGCCTC 
GTGGTGGTGC AGCAGCACCG GGCACCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8030472 ( XM_029988196.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029844056.1 Ixodes scapularis trafficking protein particle complex subunit 8 (LOC8030472), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa30113
Clone ID Related Accession (Same CDS sequence)	XM_029988196.1
Accession Version	XM_029988196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	trafficking protein particle complex subunit 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAG GCAAGCGAGG CGTCCAGGAG TTCATCCAAG GCGTCTTTGC GCCGATGATC 
ACGGCCCAGT GCAGCCCCGA CGCTGAAGAT GTGTGCCGCA GGAACAATCT TTCCTTCGTC 
GAGCTTCTAC AGCCGTTCTG CCAGGCAGTC GAAGTGACTG TTCGGCCGCC GAACACCATC 
CAGGGCTCGA TTGCCACAAA ACTTCGCCTC TGCGTTCGGG ACCTGAACAA CCCACTGCCC 
CAACTTGCGG CAGCCAAGAA GCTCCTCAAC GACTCGGTGG CCAACGTCCA GCCATCGCTC 
AATGAAAGTG GTGCATCCCT GAGCAGCATT AAAGCTGGCT GTTACAATTT GCAAGTGTCG 
AGCTCAACAC CATGGTTCCA GTCATATAGA GACACATTCT TCCGAGTGAT GCCACCGTTG 
GAGCATGATT TCACCAGGCA CTTTCTGGCT TGCATGTTTG TGATTTCCTC GGGGCATGCG 
GCTCCTCAGG AAGAGTTGCA GCGCCTGGTG CAAGCTCAGC AGCAATGCCA GGCGAATGGG 
ACACACCACT CTCGTTGGTT TTACCCTCAG ACGCTCAAGT ATTATGTGCT GATTCACGAC 
GCTCTACTTG GAAACCAGGC CAAAGCGGAG GCAACATTCC AGGGCATGAA AAAACTGTAT 
GGTGCCAGCT GCTGCCACCT ACTTCAAGTC AACTCTGCTC CCGCTGACGA TCCAACCGTC 
TCCAGTCTTC CAGACCTCTG GTCGCAGTTC TTGCATGCAG AATCCTCTGA CAAGCAGGCT 
GTGAACGACA CTTCTTCCAA GGCCCTAGGA AGCAACTTGT CTCTCGACGA CGACATCTTT 
CAACAAGAGC TTGGTAACTC CGACGATGAG ACGAGAAACC ACTGTGCCGT GCAGCACCCT 
CTCCTGAGCA GTGGTGACTC TTCTTCGCCC ACTCTGGATG TTGCGGCCAG CGAAGATAGC 
GTTTGGCGGA AGCCGGGATC CCGCCTGGGA CGCTGCCTGT CGCAGAGCGA CCGAGAGCGT 
GTGCGAGTGT TTGTCCAGGA GTTCTGCAGT CAAGGCTTGG TCCCTTACGT TGAGGAGCAG 
ATGCGCATTC TCAACGACCA GGTGACCAAC AAGAAAGGAA TCCACCGCTC ATTCATGAGC 
ATGAAGAAAC TCTTTGCTGG AAGCAAAACA GTTCCTCAAG GTCCTCATTC ATCTGTCAAC 
TCTGTCGTCT ACGGCCAAGA TGCTCCTGAA CTGCAAGTGA GACGTCTGGG CGACCTGGCC 
TTCCTCTTCC AACTGTACGA GTTTGCCTAC CAGGCCTACC ACAGTGCCAA GAGGGACTTC 
AGCAGCGACA GTGCTTGGCT TCACTTTGCC GGCGCACAGG AAATGGCTGC CCTGGCGGTG 
TTTATGGCAG GCGTGAATGC CCAGCGACCT TTCCCATTAC GTTACATGGA CTCGGCAATT 
GACCTCTACC TCAACACCTG CAAACTTCCC CAGCTGGCAA CGAGGGCAGC CCTGCTGAGC 
ACAGAGTGCC TTCGCCACCT GGGACAGCAC TCAAGCGTAG CGGCCCAGCT GATCCGGCTG 
ACGAGCGAGG ACTCTGACCT GCGCAGTGCG CTTCTCCTCG AGCAGGCGGC CTACTGCTTC 
CTTCAAGCCG GCACAGCACC CTCGGTGCGC AAGTATGCCT TCCACGTGGT GCTAGCCGGA 
CACCGCTACA GCAAGGCAGG GCACCGCAAG CACTCCCTTC GGGCCTACCA GGAGGCGCTG 
CAGGTGTACC AGGGCCGCAA CTGGAGTCTG GCAGAGGACC ACATCCACTT CACTATTGGC 
CGGCAGTCGA CATACCTCAA GCTGCTTCAG GGGGCCCACG AGGCATTCCA CTGCCTGCTG 
GCCAAGCACA GCGAGCAGTC ACCCACCCAG CAGCTGCTCT TCCTCAGGGA GTACCTCCTG 
GTGCTCAAGA TGTTGCACGA GAACGACCTG GCGGCAGCCA CGGTGCTTCT GCCGGTGCCA 
GAGGTGCACA CGAGCTCCAC GAGGGTCTAC CTGGGACCCG TGGCTGATGA GGGTGCCCTC 
TGGGGGGACC TGGGCACCTG GCTCAAGCTG GAGGAGTGTG CCGCTTCAGT GGCCAACGAC 
GGCACGCTCC CGGCCACCTT TCGTCCCCAG CTGAGTTTGC TCAGCGACGA CACCGACAAT 
GGGGCCAGGC CTCTCACAGT CGTCGGGGAA CCGGTGACAG TTGAACTAGA ACTGCGCAAT 
CCGCTCCAAA TCGCTCTCCA GCTAAGCCAG GTGCATCTGC TTTGGAGCTT TCTTCCCTCA 
GACAGGGAGG ACCTTATTAG CAATGACAGA GCTGATGCTC AGGATGGTCA GGCAGCTACA 
CTCATCAATA CTGGGATGCT GGACGAAGTT CTCCTCGAGT CGGAACAGAA GCAAAGGGTG 
CGTTTGACCT TGGTTCCCCA CTGCACGGGC GACCTCCACG TAACCGGGTT TGCCTATCGG 
GTTGGGCTCT CACCCACCCA ACTGGATGCA ACCTCGGACC TCGGACCTCT GCCCAGCCCC 
GTGAGCGGAC GCCAGTTGTT GTCAGTCCGG GGTCCGAAGC TGAAAAACCT CAAGACTGCC 
AAAAACGGAC AGTTCTACTC TCCCGACTGG AGGCTGCATC CGACGGTCGT CGCTGCCATG 
CCGCAGCTTA CGGTCAGCTT CTCCAATGTT CCCCGGCAAC TCCTCTGTGG CGAAGTGCAG 
CGCACCGTTC TACACGTTTC CAATGCGCCG AACCTCCCTG AACTCTCATC ACTTCTCATC 
GCTTCACCGA CCCCCCAACT CTTCTGCCTC GGCTTCAACG ATCCCGACGA CGAAGGCGCT 
CTGGGTTCGG CGGACGAAGG CATTGCGTAC CGTGAAAAGC CCCTGCAGTC GAGCAGGGCT 
GCACCAGTTT TAGCACAGCG ACCCACGGCA AACTTCGTCT GCAGTCTCCC AGAGGCTGTC 
TGCAGGATCC CTGGTGGTGG AGATGCCTCA GTGCCCCTGT GGATTCGGGG ACCTGACGTA 
GTGGGACGTC ACACTCTCAG ACTGCTCTTT TACTACGAGA GTGCCCAGAA GAGTAGCAGG 
CTCACGCACC GTGTTCTCTA CCACAACATC CACTTCTCAA CGCTTCCCTC GCTGGCTCTG 
CAGGCCTCAG CTCGACAGAG CGTGTCAGCA GTCGGGGCAG GTTCTCCCAA CGGACTTCTA 
GTTTCCCTTC AGGCCAAAAA CTTGAGTGAG GCTGAAAGCA TTGGAGGCGT CGACCTTAGT 
TTACTCCAGG TGACGTGCGT GAGCCGTGTT TGGAATTTGA TTCCTTTGAC GACACACCTG 
AGGGTTGGGG CAAGTGTAGT GAAACCTCAG GAAACGAAAA ACCTAGTCTT CAAAGCTGTT 
CCCTTCAGCT CCAAATTGAG CCAGGATAAC ACAGCCGTTG TTTCCCAACT ACCGTTAGCA 
CGGGTTCAGG TGGAGAGCAC GTGCACGCCG TGTCTTGATT TCTGTTGGCG AGGAAGCCAA 
GAATGGCCAC CCTTGAGCCA GGAAAAAGCA TCCTCCCAGG GAGACAATGT AGCCATGCAA 
CTTGCAGCAG CAAACTTGGA GAAGGCACCA TCCCTGGACA TTACCCTTGC AATCCTTTGG 
AAGGCTGCCA TCGAGCGAGA TGGTCAGATT AAAGTGGTCA TGGGACAGCA GCATGTGACT 
TTGAAGGACT TCCATACGCT CTTCTCGTCT GTGCCTGGAC AAAAGATGGA TCTAAGGCCA 
GCAATCCCCG TCTATCCTAC AATGGGACTT GCTGCCCCGA CGTTCCAGTA TGTTCGATGT 
GCGCTGGTTC ATCCTGTACA AGCGAACCAT GACTTTATGT CAAGCAGAAT TCTGGTGATT 
CCAGTGAGCC TCGTAGTTCA CAACTCGAGC TTTAGGACCG TCACAGTAAG CATCGAGCCG 
GCCGTCAGAA CACCACCGGC GGTCGTCCTG TCCAGTCCCG TGACCACCCC AGAGCTGGTG 
GCCACCGACG TGGGCGGTTT CAGCTGGGTA ACCGGCATCC GGCGCCACTA CCCGATGAAG 
TCGCGCGAGA CGGTCACGTT TCCGTTGACG GCCGCATTCT CCATGGCCGG GACGTACAAT 
GTGGCCGCCC TTGAAGTGAG CGTCCTCACG GACGACGGCC GCCTCTCCCA CGAGCCACTG 
TGTCCGAGCC TCGTGGTGGT GCAGCAGCAC CGGGCACCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029844056.1
CDS	286..4467
Translation	MQPGKRGVQEFIQGVFAPMITAQCSPDAEDVCRRNNLSFVELLQPFCQAVEVTVRPPNTIQGSIATKLRLCVRDLNNPLPQLAAAKKLLNDSVANVQPSLNESGASLSSIKAGCYNLQVSSSTPWFQSYRDTFFRVMPPLEHDFTRHFLACMFVISSGHAAPQEELQRLVQAQQQCQANGTHHSRWFYPQTLKYYVLIHDALLGNQAKAEATFQGMKKLYGASCCHLLQVNSAPADDPTVSSLPDLWSQFLHAESSDKQAVNDTSSKALGSNLSLDDDIFQQELGNSDDETRNHCAVQHPLLSSGDSSSPTLDVAASEDSVWRKPGSRLGRCLSQSDRERVRVFVQEFCSQGLVPYVEEQMRILNDQVTNKKGIHRSFMSMKKLFAGSKTVPQGPHSSVNSVVYGQDAPELQVRRLGDLAFLFQLYEFAYQAYHSAKRDFSSDSAWLHFAGAQEMAALAVFMAGVNAQRPFPLRYMDSAIDLYLNTCKLPQLATRAALLSTECLRHLGQHSSVAAQLIRLTSEDSDLRSALLLEQAAYCFLQAGTAPSVRKYAFHVVLAGHRYSKAGHRKHSLRAYQEALQVYQGRNWSLAEDHIHFTIGRQSTYLKLLQGAHEAFHCLLAKHSEQSPTQQLLFLREYLLVLKMLHENDLAAATVLLPVPEVHTSSTRVYLGPVADEGALWGDLGTWLKLEECAASVANDGTLPATFRPQLSLLSDDTDNGARPLTVVGEPVTVELELRNPLQIALQLSQVHLLWSFLPSDREDLISNDRADAQDGQAATLINTGMLDEVLLESEQKQRVRLTLVPHCTGDLHVTGFAYRVGLSPTQLDATSDLGPLPSPVSGRQLLSVRGPKLKNLKTAKNGQFYSPDWRLHPTVVAAMPQLTVSFSNVPRQLLCGEVQRTVLHVSNAPNLPELSSLLIASPTPQLFCLGFNDPDDEGALGSADEGIAYREKPLQSSRAAPVLAQRPTANFVCSLPEAVCRIPGGGDASVPLWIRGPDVVGRHTLRLLFYYESAQKSSRLTHRVLYHNIHFSTLPSLALQASARQSVSAVGAGSPNGLLVSLQAKNLSEAESIGGVDLSLLQVTCVSRVWNLIPLTTHLRVGASVVKPQETKNLVFKAVPFSSKLSQDNTAVVSQLPLARVQVESTCTPCLDFCWRGSQEWPPLSQEKASSQGDNVAMQLAAANLEKAPSLDITLAILWKAAIERDGQIKVVMGQQHVTLKDFHTLFSSVPGQKMDLRPAIPVYPTMGLAAPTFQYVRCALVHPVQANHDFMSSRILVIPVSLVVHNSSFRTVTVSIEPAVRTPPAVVLSSPVTTPELVATDVGGFSWVTGIRRHYPMKSRETVTFPLTAAFSMAGTYNVAALEVSVLTDDGRLSHEPLCPSLVVVQQHRAP

Target ORF information:

RefSeq Version	XM_029988196.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis trafficking protein particle complex subunit 8 (LOC8030472), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029988196.1

ORF Insert Sequence:

ATGCAGCCAG GCAAGCGAGG CGTCCAGGAG TTCATCCAAG GCGTCTTTGC GCCGATGATC 
ACGGCCCAGT GCAGCCCCGA CGCTGAAGAT GTGTGCCGCA GGAACAATCT TTCCTTCGTC 
GAGCTTCTAC AGCCGTTCTG CCAGGCAGTC GAAGTGACTG TTCGGCCGCC GAACACCATC 
CAGGGCTCGA TTGCCACAAA ACTTCGCCTC TGCGTTCGGG ACCTGAACAA CCCACTGCCC 
CAACTTGCGG CAGCCAAGAA GCTCCTCAAC GACTCGGTGG CCAACGTCCA GCCATCGCTC 
AATGAAAGTG GTGCATCCCT GAGCAGCATT AAAGCTGGCT GTTACAATTT GCAAGTGTCG 
AGCTCAACAC CATGGTTCCA GTCATATAGA GACACATTCT TCCGAGTGAT GCCACCGTTG 
GAGCATGATT TCACCAGGCA CTTTCTGGCT TGCATGTTTG TGATTTCCTC GGGGCATGCG 
GCTCCTCAGG AAGAGTTGCA GCGCCTGGTG CAAGCTCAGC AGCAATGCCA GGCGAATGGG 
ACACACCACT CTCGTTGGTT TTACCCTCAG ACGCTCAAGT ATTATGTGCT GATTCACGAC 
GCTCTACTTG GAAACCAGGC CAAAGCGGAG GCAACATTCC AGGGCATGAA AAAACTGTAT 
GGTGCCAGCT GCTGCCACCT ACTTCAAGTC AACTCTGCTC CCGCTGACGA TCCAACCGTC 
TCCAGTCTTC CAGACCTCTG GTCGCAGTTC TTGCATGCAG AATCCTCTGA CAAGCAGGCT 
GTGAACGACA CTTCTTCCAA GGCCCTAGGA AGCAACTTGT CTCTCGACGA CGACATCTTT 
CAACAAGAGC TTGGTAACTC CGACGATGAG ACGAGAAACC ACTGTGCCGT GCAGCACCCT 
CTCCTGAGCA GTGGTGACTC TTCTTCGCCC ACTCTGGATG TTGCGGCCAG CGAAGATAGC 
GTTTGGCGGA AGCCGGGATC CCGCCTGGGA CGCTGCCTGT CGCAGAGCGA CCGAGAGCGT 
GTGCGAGTGT TTGTCCAGGA GTTCTGCAGT CAAGGCTTGG TCCCTTACGT TGAGGAGCAG 
ATGCGCATTC TCAACGACCA GGTGACCAAC AAGAAAGGAA TCCACCGCTC ATTCATGAGC 
ATGAAGAAAC TCTTTGCTGG AAGCAAAACA GTTCCTCAAG GTCCTCATTC ATCTGTCAAC 
TCTGTCGTCT ACGGCCAAGA TGCTCCTGAA CTGCAAGTGA GACGTCTGGG CGACCTGGCC 
TTCCTCTTCC AACTGTACGA GTTTGCCTAC CAGGCCTACC ACAGTGCCAA GAGGGACTTC 
AGCAGCGACA GTGCTTGGCT TCACTTTGCC GGCGCACAGG AAATGGCTGC CCTGGCGGTG 
TTTATGGCAG GCGTGAATGC CCAGCGACCT TTCCCATTAC GTTACATGGA CTCGGCAATT 
GACCTCTACC TCAACACCTG CAAACTTCCC CAGCTGGCAA CGAGGGCAGC CCTGCTGAGC 
ACAGAGTGCC TTCGCCACCT GGGACAGCAC TCAAGCGTAG CGGCCCAGCT GATCCGGCTG 
ACGAGCGAGG ACTCTGACCT GCGCAGTGCG CTTCTCCTCG AGCAGGCGGC CTACTGCTTC 
CTTCAAGCCG GCACAGCACC CTCGGTGCGC AAGTATGCCT TCCACGTGGT GCTAGCCGGA 
CACCGCTACA GCAAGGCAGG GCACCGCAAG CACTCCCTTC GGGCCTACCA GGAGGCGCTG 
CAGGTGTACC AGGGCCGCAA CTGGAGTCTG GCAGAGGACC ACATCCACTT CACTATTGGC 
CGGCAGTCGA CATACCTCAA GCTGCTTCAG GGGGCCCACG AGGCATTCCA CTGCCTGCTG 
GCCAAGCACA GCGAGCAGTC ACCCACCCAG CAGCTGCTCT TCCTCAGGGA GTACCTCCTG 
GTGCTCAAGA TGTTGCACGA GAACGACCTG GCGGCAGCCA CGGTGCTTCT GCCGGTGCCA 
GAGGTGCACA CGAGCTCCAC GAGGGTCTAC CTGGGACCCG TGGCTGATGA GGGTGCCCTC 
TGGGGGGACC TGGGCACCTG GCTCAAGCTG GAGGAGTGTG CCGCTTCAGT GGCCAACGAC 
GGCACGCTCC CGGCCACCTT TCGTCCCCAG CTGAGTTTGC TCAGCGACGA CACCGACAAT 
GGGGCCAGGC CTCTCACAGT CGTCGGGGAA CCGGTGACAG TTGAACTAGA ACTGCGCAAT 
CCGCTCCAAA TCGCTCTCCA GCTAAGCCAG GTGCATCTGC TTTGGAGCTT TCTTCCCTCA 
GACAGGGAGG ACCTTATTAG CAATGACAGA GCTGATGCTC AGGATGGTCA GGCAGCTACA 
CTCATCAATA CTGGGATGCT GGACGAAGTT CTCCTCGAGT CGGAACAGAA GCAAAGGGTG 
CGTTTGACCT TGGTTCCCCA CTGCACGGGC GACCTCCACG TAACCGGGTT TGCCTATCGG 
GTTGGGCTCT CACCCACCCA ACTGGATGCA ACCTCGGACC TCGGACCTCT GCCCAGCCCC 
GTGAGCGGAC GCCAGTTGTT GTCAGTCCGG GGTCCGAAGC TGAAAAACCT CAAGACTGCC 
AAAAACGGAC AGTTCTACTC TCCCGACTGG AGGCTGCATC CGACGGTCGT CGCTGCCATG 
CCGCAGCTTA CGGTCAGCTT CTCCAATGTT CCCCGGCAAC TCCTCTGTGG CGAAGTGCAG 
CGCACCGTTC TACACGTTTC CAATGCGCCG AACCTCCCTG AACTCTCATC ACTTCTCATC 
GCTTCACCGA CCCCCCAACT CTTCTGCCTC GGCTTCAACG ATCCCGACGA CGAAGGCGCT 
CTGGGTTCGG CGGACGAAGG CATTGCGTAC CGTGAAAAGC CCCTGCAGTC GAGCAGGGCT 
GCACCAGTTT TAGCACAGCG ACCCACGGCA AACTTCGTCT GCAGTCTCCC AGAGGCTGTC 
TGCAGGATCC CTGGTGGTGG AGATGCCTCA GTGCCCCTGT GGATTCGGGG ACCTGACGTA 
GTGGGACGTC ACACTCTCAG ACTGCTCTTT TACTACGAGA GTGCCCAGAA GAGTAGCAGG 
CTCACGCACC GTGTTCTCTA CCACAACATC CACTTCTCAA CGCTTCCCTC GCTGGCTCTG 
CAGGCCTCAG CTCGACAGAG CGTGTCAGCA GTCGGGGCAG GTTCTCCCAA CGGACTTCTA 
GTTTCCCTTC AGGCCAAAAA CTTGAGTGAG GCTGAAAGCA TTGGAGGCGT CGACCTTAGT 
TTACTCCAGG TGACGTGCGT GAGCCGTGTT TGGAATTTGA TTCCTTTGAC GACACACCTG 
AGGGTTGGGG CAAGTGTAGT GAAACCTCAG GAAACGAAAA ACCTAGTCTT CAAAGCTGTT 
CCCTTCAGCT CCAAATTGAG CCAGGATAAC ACAGCCGTTG TTTCCCAACT ACCGTTAGCA 
CGGGTTCAGG TGGAGAGCAC GTGCACGCCG TGTCTTGATT TCTGTTGGCG AGGAAGCCAA 
GAATGGCCAC CCTTGAGCCA GGAAAAAGCA TCCTCCCAGG GAGACAATGT AGCCATGCAA 
CTTGCAGCAG CAAACTTGGA GAAGGCACCA TCCCTGGACA TTACCCTTGC AATCCTTTGG 
AAGGCTGCCA TCGAGCGAGA TGGTCAGATT AAAGTGGTCA TGGGACAGCA GCATGTGACT 
TTGAAGGACT TCCATACGCT CTTCTCGTCT GTGCCTGGAC AAAAGATGGA TCTAAGGCCA 
GCAATCCCCG TCTATCCTAC AATGGGACTT GCTGCCCCGA CGTTCCAGTA TGTTCGATGT 
GCGCTGGTTC ATCCTGTACA AGCGAACCAT GACTTTATGT CAAGCAGAAT TCTGGTGATT 
CCAGTGAGCC TCGTAGTTCA CAACTCGAGC TTTAGGACCG TCACAGTAAG CATCGAGCCG 
GCCGTCAGAA CACCACCGGC GGTCGTCCTG TCCAGTCCCG TGACCACCCC AGAGCTGGTG 
GCCACCGACG TGGGCGGTTT CAGCTGGGTA ACCGGCATCC GGCGCCACTA CCCGATGAAG 
TCGCGCGAGA CGGTCACGTT TCCGTTGACG GCCGCATTCT CCATGGCCGG GACGTACAAT 
GTGGCCGCCC TTGAAGTGAG CGTCCTCACG GACGACGGCC GCCTCTCCCA CGAGCCACTG 
TGTCCGAGCC TCGTGGTGGT GCAGCAGCAC CGGGCACCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8030472 ( XM_029988197.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029844057.1 Ixodes scapularis trafficking protein particle complex subunit 8 (LOC8030472), transcript variant X2, mRNA.

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CloneID	OIa30114
Clone ID Related Accession (Same CDS sequence)	XM_029988197.1
Accession Version	XM_029988197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	trafficking protein particle complex subunit 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAG GCAAGCGAGG CGTCCAGGAG TTCATCCAAG GCGTCTTTGC GCCGATGATC 
ACGGCCCAGT GCAGCCCCGA CGCTGAAGAT GTGTGCCGCA GGAACAATCT TTCCTTCGTC 
GAGCTTCTAC AGCCGTTCTG CCAGGCAGTC GAAGTGACTG TTCGGCCGCC GAACACCATC 
CAGGGCTCGA TTGCCACAAA ACTTCGCCTC TGCGTTCGGG ACCTGAACAA CCCACTGCCC 
CAACTTGCGG CAGCCAAGAA GCTCCTCAAC GACTCGGTGG CCAACGTCCA GCCATCGCTC 
AATGAAAGTG GTGCATCCCT GAGCAGCATT AAAGCTGGCT GTTACAATTT GCAAGTGTCG 
AGCTCAACAC CATGGTTCCA GTCATATAGA GACACATTCT TCCGAGTGAT GCCACCGTTG 
GAGCATGATT TCACCAGGCA CTTTCTGGCT TGCATGTTTG TGATTTCCTC GGGGCATGCG 
GCTCCTCAGG AAGAGTTGCA GCGCCTGGTG CAAGCTCAGC AGCAATGCCA GGCGAATGGG 
ACACACCACT CTCGTTGGTT TTACCCTCAG ACGCTCAAGT ATTATGTGCT GATTCACGAC 
GCTCTACTTG GAAACCAGGC CAAAGCGGAG GCAACATTCC AGGGCATGAA AAAACTGTAT 
GGTGCCAGCT GCTGCCACCT ACTTCAAGTC AACTCTGCTC CCGCTGACGA TCCAACCGTC 
TCCAGTCTTC CAGACCTCTG GTCGCAGTTC TTGCATGCAG AATCCTCTGA CAAGGCTGTG 
AACGACACTT CTTCCAAGGC CCTAGGAAGC AACTTGTCTC TCGACGACGA CATCTTTCAA 
CAAGAGCTTG GTAACTCCGA CGATGAGACG AGAAACCACT GTGCCGTGCA GCACCCTCTC 
CTGAGCAGTG GTGACTCTTC TTCGCCCACT CTGGATGTTG CGGCCAGCGA AGATAGCGTT 
TGGCGGAAGC CGGGATCCCG CCTGGGACGC TGCCTGTCGC AGAGCGACCG AGAGCGTGTG 
CGAGTGTTTG TCCAGGAGTT CTGCAGTCAA GGCTTGGTCC CTTACGTTGA GGAGCAGATG 
CGCATTCTCA ACGACCAGGT GACCAACAAG AAAGGAATCC ACCGCTCATT CATGAGCATG 
AAGAAACTCT TTGCTGGAAG CAAAACAGTT CCTCAAGGTC CTCATTCATC TGTCAACTCT 
GTCGTCTACG GCCAAGATGC TCCTGAACTG CAAGTGAGAC GTCTGGGCGA CCTGGCCTTC 
CTCTTCCAAC TGTACGAGTT TGCCTACCAG GCCTACCACA GTGCCAAGAG GGACTTCAGC 
AGCGACAGTG CTTGGCTTCA CTTTGCCGGC GCACAGGAAA TGGCTGCCCT GGCGGTGTTT 
ATGGCAGGCG TGAATGCCCA GCGACCTTTC CCATTACGTT ACATGGACTC GGCAATTGAC 
CTCTACCTCA ACACCTGCAA ACTTCCCCAG CTGGCAACGA GGGCAGCCCT GCTGAGCACA 
GAGTGCCTTC GCCACCTGGG ACAGCACTCA AGCGTAGCGG CCCAGCTGAT CCGGCTGACG 
AGCGAGGACT CTGACCTGCG CAGTGCGCTT CTCCTCGAGC AGGCGGCCTA CTGCTTCCTT 
CAAGCCGGCA CAGCACCCTC GGTGCGCAAG TATGCCTTCC ACGTGGTGCT AGCCGGACAC 
CGCTACAGCA AGGCAGGGCA CCGCAAGCAC TCCCTTCGGG CCTACCAGGA GGCGCTGCAG 
GTGTACCAGG GCCGCAACTG GAGTCTGGCA GAGGACCACA TCCACTTCAC TATTGGCCGG 
CAGTCGACAT ACCTCAAGCT GCTTCAGGGG GCCCACGAGG CATTCCACTG CCTGCTGGCC 
AAGCACAGCG AGCAGTCACC CACCCAGCAG CTGCTCTTCC TCAGGGAGTA CCTCCTGGTG 
CTCAAGATGT TGCACGAGAA CGACCTGGCG GCAGCCACGG TGCTTCTGCC GGTGCCAGAG 
GTGCACACGA GCTCCACGAG GGTCTACCTG GGACCCGTGG CTGATGAGGG TGCCCTCTGG 
GGGGACCTGG GCACCTGGCT CAAGCTGGAG GAGTGTGCCG CTTCAGTGGC CAACGACGGC 
ACGCTCCCGG CCACCTTTCG TCCCCAGCTG AGTTTGCTCA GCGACGACAC CGACAATGGG 
GCCAGGCCTC TCACAGTCGT CGGGGAACCG GTGACAGTTG AACTAGAACT GCGCAATCCG 
CTCCAAATCG CTCTCCAGCT AAGCCAGGTG CATCTGCTTT GGAGCTTTCT TCCCTCAGAC 
AGGGAGGACC TTATTAGCAA TGACAGAGCT GATGCTCAGG ATGGTCAGGC AGCTACACTC 
ATCAATACTG GGATGCTGGA CGAAGTTCTC CTCGAGTCGG AACAGAAGCA AAGGGTGCGT 
TTGACCTTGG TTCCCCACTG CACGGGCGAC CTCCACGTAA CCGGGTTTGC CTATCGGGTT 
GGGCTCTCAC CCACCCAACT GGATGCAACC TCGGACCTCG GACCTCTGCC CAGCCCCGTG 
AGCGGACGCC AGTTGTTGTC AGTCCGGGGT CCGAAGCTGA AAAACCTCAA GACTGCCAAA 
AACGGACAGT TCTACTCTCC CGACTGGAGG CTGCATCCGA CGGTCGTCGC TGCCATGCCG 
CAGCTTACGG TCAGCTTCTC CAATGTTCCC CGGCAACTCC TCTGTGGCGA AGTGCAGCGC 
ACCGTTCTAC ACGTTTCCAA TGCGCCGAAC CTCCCTGAAC TCTCATCACT TCTCATCGCT 
TCACCGACCC CCCAACTCTT CTGCCTCGGC TTCAACGATC CCGACGACGA AGGCGCTCTG 
GGTTCGGCGG ACGAAGGCAT TGCGTACCGT GAAAAGCCCC TGCAGTCGAG CAGGGCTGCA 
CCAGTTTTAG CACAGCGACC CACGGCAAAC TTCGTCTGCA GTCTCCCAGA GGCTGTCTGC 
AGGATCCCTG GTGGTGGAGA TGCCTCAGTG CCCCTGTGGA TTCGGGGACC TGACGTAGTG 
GGACGTCACA CTCTCAGACT GCTCTTTTAC TACGAGAGTG CCCAGAAGAG TAGCAGGCTC 
ACGCACCGTG TTCTCTACCA CAACATCCAC TTCTCAACGC TTCCCTCGCT GGCTCTGCAG 
GCCTCAGCTC GACAGAGCGT GTCAGCAGTC GGGGCAGGTT CTCCCAACGG ACTTCTAGTT 
TCCCTTCAGG CCAAAAACTT GAGTGAGGCT GAAAGCATTG GAGGCGTCGA CCTTAGTTTA 
CTCCAGGTGA CGTGCGTGAG CCGTGTTTGG AATTTGATTC CTTTGACGAC ACACCTGAGG 
GTTGGGGCAA GTGTAGTGAA ACCTCAGGAA ACGAAAAACC TAGTCTTCAA AGCTGTTCCC 
TTCAGCTCCA AATTGAGCCA GGATAACACA GCCGTTGTTT CCCAACTACC GTTAGCACGG 
GTTCAGGTGG AGAGCACGTG CACGCCGTGT CTTGATTTCT GTTGGCGAGG AAGCCAAGAA 
TGGCCACCCT TGAGCCAGGA AAAAGCATCC TCCCAGGGAG ACAATGTAGC CATGCAACTT 
GCAGCAGCAA ACTTGGAGAA GGCACCATCC CTGGACATTA CCCTTGCAAT CCTTTGGAAG 
GCTGCCATCG AGCGAGATGG TCAGATTAAA GTGGTCATGG GACAGCAGCA TGTGACTTTG 
AAGGACTTCC ATACGCTCTT CTCGTCTGTG CCTGGACAAA AGATGGATCT AAGGCCAGCA 
ATCCCCGTCT ATCCTACAAT GGGACTTGCT GCCCCGACGT TCCAGTATGT TCGATGTGCG 
CTGGTTCATC CTGTACAAGC GAACCATGAC TTTATGTCAA GCAGAATTCT GGTGATTCCA 
GTGAGCCTCG TAGTTCACAA CTCGAGCTTT AGGACCGTCA CAGTAAGCAT CGAGCCGGCC 
GTCAGAACAC CACCGGCGGT CGTCCTGTCC AGTCCCGTGA CCACCCCAGA GCTGGTGGCC 
ACCGACGTGG GCGGTTTCAG CTGGGTAACC GGCATCCGGC GCCACTACCC GATGAAGTCG 
CGCGAGACGG TCACGTTTCC GTTGACGGCC GCATTCTCCA TGGCCGGGAC GTACAATGTG 
GCCGCCCTTG AAGTGAGCGT CCTCACGGAC GACGGCCGCC TCTCCCACGA GCCACTGTGT 
CCGAGCCTCG TGGTGGTGCA GCAGCACCGG GCACCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029844057.1
CDS	286..4464
Translation	MQPGKRGVQEFIQGVFAPMITAQCSPDAEDVCRRNNLSFVELLQPFCQAVEVTVRPPNTIQGSIATKLRLCVRDLNNPLPQLAAAKKLLNDSVANVQPSLNESGASLSSIKAGCYNLQVSSSTPWFQSYRDTFFRVMPPLEHDFTRHFLACMFVISSGHAAPQEELQRLVQAQQQCQANGTHHSRWFYPQTLKYYVLIHDALLGNQAKAEATFQGMKKLYGASCCHLLQVNSAPADDPTVSSLPDLWSQFLHAESSDKAVNDTSSKALGSNLSLDDDIFQQELGNSDDETRNHCAVQHPLLSSGDSSSPTLDVAASEDSVWRKPGSRLGRCLSQSDRERVRVFVQEFCSQGLVPYVEEQMRILNDQVTNKKGIHRSFMSMKKLFAGSKTVPQGPHSSVNSVVYGQDAPELQVRRLGDLAFLFQLYEFAYQAYHSAKRDFSSDSAWLHFAGAQEMAALAVFMAGVNAQRPFPLRYMDSAIDLYLNTCKLPQLATRAALLSTECLRHLGQHSSVAAQLIRLTSEDSDLRSALLLEQAAYCFLQAGTAPSVRKYAFHVVLAGHRYSKAGHRKHSLRAYQEALQVYQGRNWSLAEDHIHFTIGRQSTYLKLLQGAHEAFHCLLAKHSEQSPTQQLLFLREYLLVLKMLHENDLAAATVLLPVPEVHTSSTRVYLGPVADEGALWGDLGTWLKLEECAASVANDGTLPATFRPQLSLLSDDTDNGARPLTVVGEPVTVELELRNPLQIALQLSQVHLLWSFLPSDREDLISNDRADAQDGQAATLINTGMLDEVLLESEQKQRVRLTLVPHCTGDLHVTGFAYRVGLSPTQLDATSDLGPLPSPVSGRQLLSVRGPKLKNLKTAKNGQFYSPDWRLHPTVVAAMPQLTVSFSNVPRQLLCGEVQRTVLHVSNAPNLPELSSLLIASPTPQLFCLGFNDPDDEGALGSADEGIAYREKPLQSSRAAPVLAQRPTANFVCSLPEAVCRIPGGGDASVPLWIRGPDVVGRHTLRLLFYYESAQKSSRLTHRVLYHNIHFSTLPSLALQASARQSVSAVGAGSPNGLLVSLQAKNLSEAESIGGVDLSLLQVTCVSRVWNLIPLTTHLRVGASVVKPQETKNLVFKAVPFSSKLSQDNTAVVSQLPLARVQVESTCTPCLDFCWRGSQEWPPLSQEKASSQGDNVAMQLAAANLEKAPSLDITLAILWKAAIERDGQIKVVMGQQHVTLKDFHTLFSSVPGQKMDLRPAIPVYPTMGLAAPTFQYVRCALVHPVQANHDFMSSRILVIPVSLVVHNSSFRTVTVSIEPAVRTPPAVVLSSPVTTPELVATDVGGFSWVTGIRRHYPMKSRETVTFPLTAAFSMAGTYNVAALEVSVLTDDGRLSHEPLCPSLVVVQQHRAP

Target ORF information:

RefSeq Version	XM_029988197.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis trafficking protein particle complex subunit 8 (LOC8030472), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029988197.1

ORF Insert Sequence:

ATGCAGCCAG GCAAGCGAGG CGTCCAGGAG TTCATCCAAG GCGTCTTTGC GCCGATGATC 
ACGGCCCAGT GCAGCCCCGA CGCTGAAGAT GTGTGCCGCA GGAACAATCT TTCCTTCGTC 
GAGCTTCTAC AGCCGTTCTG CCAGGCAGTC GAAGTGACTG TTCGGCCGCC GAACACCATC 
CAGGGCTCGA TTGCCACAAA ACTTCGCCTC TGCGTTCGGG ACCTGAACAA CCCACTGCCC 
CAACTTGCGG CAGCCAAGAA GCTCCTCAAC GACTCGGTGG CCAACGTCCA GCCATCGCTC 
AATGAAAGTG GTGCATCCCT GAGCAGCATT AAAGCTGGCT GTTACAATTT GCAAGTGTCG 
AGCTCAACAC CATGGTTCCA GTCATATAGA GACACATTCT TCCGAGTGAT GCCACCGTTG 
GAGCATGATT TCACCAGGCA CTTTCTGGCT TGCATGTTTG TGATTTCCTC GGGGCATGCG 
GCTCCTCAGG AAGAGTTGCA GCGCCTGGTG CAAGCTCAGC AGCAATGCCA GGCGAATGGG 
ACACACCACT CTCGTTGGTT TTACCCTCAG ACGCTCAAGT ATTATGTGCT GATTCACGAC 
GCTCTACTTG GAAACCAGGC CAAAGCGGAG GCAACATTCC AGGGCATGAA AAAACTGTAT 
GGTGCCAGCT GCTGCCACCT ACTTCAAGTC AACTCTGCTC CCGCTGACGA TCCAACCGTC 
TCCAGTCTTC CAGACCTCTG GTCGCAGTTC TTGCATGCAG AATCCTCTGA CAAGGCTGTG 
AACGACACTT CTTCCAAGGC CCTAGGAAGC AACTTGTCTC TCGACGACGA CATCTTTCAA 
CAAGAGCTTG GTAACTCCGA CGATGAGACG AGAAACCACT GTGCCGTGCA GCACCCTCTC 
CTGAGCAGTG GTGACTCTTC TTCGCCCACT CTGGATGTTG CGGCCAGCGA AGATAGCGTT 
TGGCGGAAGC CGGGATCCCG CCTGGGACGC TGCCTGTCGC AGAGCGACCG AGAGCGTGTG 
CGAGTGTTTG TCCAGGAGTT CTGCAGTCAA GGCTTGGTCC CTTACGTTGA GGAGCAGATG 
CGCATTCTCA ACGACCAGGT GACCAACAAG AAAGGAATCC ACCGCTCATT CATGAGCATG 
AAGAAACTCT TTGCTGGAAG CAAAACAGTT CCTCAAGGTC CTCATTCATC TGTCAACTCT 
GTCGTCTACG GCCAAGATGC TCCTGAACTG CAAGTGAGAC GTCTGGGCGA CCTGGCCTTC 
CTCTTCCAAC TGTACGAGTT TGCCTACCAG GCCTACCACA GTGCCAAGAG GGACTTCAGC 
AGCGACAGTG CTTGGCTTCA CTTTGCCGGC GCACAGGAAA TGGCTGCCCT GGCGGTGTTT 
ATGGCAGGCG TGAATGCCCA GCGACCTTTC CCATTACGTT ACATGGACTC GGCAATTGAC 
CTCTACCTCA ACACCTGCAA ACTTCCCCAG CTGGCAACGA GGGCAGCCCT GCTGAGCACA 
GAGTGCCTTC GCCACCTGGG ACAGCACTCA AGCGTAGCGG CCCAGCTGAT CCGGCTGACG 
AGCGAGGACT CTGACCTGCG CAGTGCGCTT CTCCTCGAGC AGGCGGCCTA CTGCTTCCTT 
CAAGCCGGCA CAGCACCCTC GGTGCGCAAG TATGCCTTCC ACGTGGTGCT AGCCGGACAC 
CGCTACAGCA AGGCAGGGCA CCGCAAGCAC TCCCTTCGGG CCTACCAGGA GGCGCTGCAG 
GTGTACCAGG GCCGCAACTG GAGTCTGGCA GAGGACCACA TCCACTTCAC TATTGGCCGG 
CAGTCGACAT ACCTCAAGCT GCTTCAGGGG GCCCACGAGG CATTCCACTG CCTGCTGGCC 
AAGCACAGCG AGCAGTCACC CACCCAGCAG CTGCTCTTCC TCAGGGAGTA CCTCCTGGTG 
CTCAAGATGT TGCACGAGAA CGACCTGGCG GCAGCCACGG TGCTTCTGCC GGTGCCAGAG 
GTGCACACGA GCTCCACGAG GGTCTACCTG GGACCCGTGG CTGATGAGGG TGCCCTCTGG 
GGGGACCTGG GCACCTGGCT CAAGCTGGAG GAGTGTGCCG CTTCAGTGGC CAACGACGGC 
ACGCTCCCGG CCACCTTTCG TCCCCAGCTG AGTTTGCTCA GCGACGACAC CGACAATGGG 
GCCAGGCCTC TCACAGTCGT CGGGGAACCG GTGACAGTTG AACTAGAACT GCGCAATCCG 
CTCCAAATCG CTCTCCAGCT AAGCCAGGTG CATCTGCTTT GGAGCTTTCT TCCCTCAGAC 
AGGGAGGACC TTATTAGCAA TGACAGAGCT GATGCTCAGG ATGGTCAGGC AGCTACACTC 
ATCAATACTG GGATGCTGGA CGAAGTTCTC CTCGAGTCGG AACAGAAGCA AAGGGTGCGT 
TTGACCTTGG TTCCCCACTG CACGGGCGAC CTCCACGTAA CCGGGTTTGC CTATCGGGTT 
GGGCTCTCAC CCACCCAACT GGATGCAACC TCGGACCTCG GACCTCTGCC CAGCCCCGTG 
AGCGGACGCC AGTTGTTGTC AGTCCGGGGT CCGAAGCTGA AAAACCTCAA GACTGCCAAA 
AACGGACAGT TCTACTCTCC CGACTGGAGG CTGCATCCGA CGGTCGTCGC TGCCATGCCG 
CAGCTTACGG TCAGCTTCTC CAATGTTCCC CGGCAACTCC TCTGTGGCGA AGTGCAGCGC 
ACCGTTCTAC ACGTTTCCAA TGCGCCGAAC CTCCCTGAAC TCTCATCACT TCTCATCGCT 
TCACCGACCC CCCAACTCTT CTGCCTCGGC TTCAACGATC CCGACGACGA AGGCGCTCTG 
GGTTCGGCGG ACGAAGGCAT TGCGTACCGT GAAAAGCCCC TGCAGTCGAG CAGGGCTGCA 
CCAGTTTTAG CACAGCGACC CACGGCAAAC TTCGTCTGCA GTCTCCCAGA GGCTGTCTGC 
AGGATCCCTG GTGGTGGAGA TGCCTCAGTG CCCCTGTGGA TTCGGGGACC TGACGTAGTG 
GGACGTCACA CTCTCAGACT GCTCTTTTAC TACGAGAGTG CCCAGAAGAG TAGCAGGCTC 
ACGCACCGTG TTCTCTACCA CAACATCCAC TTCTCAACGC TTCCCTCGCT GGCTCTGCAG 
GCCTCAGCTC GACAGAGCGT GTCAGCAGTC GGGGCAGGTT CTCCCAACGG ACTTCTAGTT 
TCCCTTCAGG CCAAAAACTT GAGTGAGGCT GAAAGCATTG GAGGCGTCGA CCTTAGTTTA 
CTCCAGGTGA CGTGCGTGAG CCGTGTTTGG AATTTGATTC CTTTGACGAC ACACCTGAGG 
GTTGGGGCAA GTGTAGTGAA ACCTCAGGAA ACGAAAAACC TAGTCTTCAA AGCTGTTCCC 
TTCAGCTCCA AATTGAGCCA GGATAACACA GCCGTTGTTT CCCAACTACC GTTAGCACGG 
GTTCAGGTGG AGAGCACGTG CACGCCGTGT CTTGATTTCT GTTGGCGAGG AAGCCAAGAA 
TGGCCACCCT TGAGCCAGGA AAAAGCATCC TCCCAGGGAG ACAATGTAGC CATGCAACTT 
GCAGCAGCAA ACTTGGAGAA GGCACCATCC CTGGACATTA CCCTTGCAAT CCTTTGGAAG 
GCTGCCATCG AGCGAGATGG TCAGATTAAA GTGGTCATGG GACAGCAGCA TGTGACTTTG 
AAGGACTTCC ATACGCTCTT CTCGTCTGTG CCTGGACAAA AGATGGATCT AAGGCCAGCA 
ATCCCCGTCT ATCCTACAAT GGGACTTGCT GCCCCGACGT TCCAGTATGT TCGATGTGCG 
CTGGTTCATC CTGTACAAGC GAACCATGAC TTTATGTCAA GCAGAATTCT GGTGATTCCA 
GTGAGCCTCG TAGTTCACAA CTCGAGCTTT AGGACCGTCA CAGTAAGCAT CGAGCCGGCC 
GTCAGAACAC CACCGGCGGT CGTCCTGTCC AGTCCCGTGA CCACCCCAGA GCTGGTGGCC 
ACCGACGTGG GCGGTTTCAG CTGGGTAACC GGCATCCGGC GCCACTACCC GATGAAGTCG 
CGCGAGACGG TCACGTTTCC GTTGACGGCC GCATTCTCCA TGGCCGGGAC GTACAATGTG 
GCCGCCCTTG AAGTGAGCGT CCTCACGGAC GACGGCCGCC TCTCCCACGA GCCACTGTGT 
CCGAGCCTCG TGGTGGTGCA GCAGCACCGG GCACCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8030472 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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