LOC8032044 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8032044
Entrez Gene ID	8032044
Full Name	Rho/RAC guanine nucleotide exchange factor, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029987662.1	XP_029843522.1	intersectin-1 isoform X3
XM_029987663.1	XP_029843523.1	intersectin-1 isoform X4
XM_029987659.1	XP_029843519.1	intersectin-1 isoform X1
XM_029987660.1	XP_029843520.1	intersectin-1 isoform X1
XM_002408314.1	XP_002408358.1	Rho/RAC guanine nucleotide exchange factor, putative
XM_029987661.1	XP_029843521.1	intersectin-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC8032044 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8032044 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa05025	XM_002408314.1 Latest version!	Ixodes scapularis Rho/RAC guanine nucleotide exchange factor, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OIa29638	XM_029987660.1 Latest version!	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29638	XM_029987659.1 Latest version!	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29639	XM_029987661.1 Latest version!	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29640	XM_029987662.1 Latest version!	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29641	XM_029987663.1 Latest version!	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8032044 ( XM_002408314.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002408358.1 Ixodes scapularis Rho/RAC guanine nucleotide exchange factor, putative, mRNA.

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CloneID	OIa05025
Clone ID Related Accession (Same CDS sequence)	XM_002408314.1
Accession Version	XM_002408314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2009-06-22
Organism	Ixodes scapularis(black-legged tick)
Product	Rho/RAC guanine nucleotide exchange factor, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002694441). COMPLETENESS: incomplete on the 5' end.

ATGAAGCTGA CTAAGAAGCC AGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGAG 
CAAGTGCGTG CCCTGTTCCC ATTTGTGGCC CAACACGAGG ACGAGCTGTC ATTCCAGAAG 
GGTCAGGTGG TGACGGTGCT GAGCAAGGAG GACCCCTCTT GGTGGAAGGG GGAGCTGGGC 
GGACACGTGG GACTCTTCCC TTCCAACTAC GTCGAGCCAC TGGATCGGGC TGACAAGTGT 
AAGTGCCCCC TCCCTGACAT TTTGTTCAGC TCCTCCCTGA GCACGCGCGA GAAGAAGAGG 
CAAGGCCACA TCTACGAGCT CCTGTCCACG GAAGAGAACT ACCTGAAGGA CCTGACCCTA 
GTCAAGGAGG TTTTCTACAG GCCAATGAAA CAGTCCACTC TTCTAACGGA CGACGAGGTT 
AAACTGATCT TCGTAAACTG GCCAGAGCTC ATCATGTGCA ACACCAAGAT GCTCAAATCG 
TTTCGGGTAC GACAGCGAAT GAGCGAGAAC GGCATCATAG AGATGATTGG AGACATCCTT 
TGTGAAAATC TACCGTACCT TAACCCGTAC GTGAGGTTCT GCAGCTGCCA GGTCCGATCC 
ATGGCCTTCA TTCAACAGAA ATCCGAAAGC GATCCCCGCT TCAAATCCTT CACCAGGGAT 
TTCTGCAAGA ACCCGAAGAC CCGCGGCATG CCTTTCACCA GCTTCCTTCT GAAGCCAATG 
CAGAGGATGA CCAAGTACCC CCTCATGATT AAAAAGATTC GCGAGTACAC GGACCCGACG 
CACCACGACC GCGGCTACCT GGACGAGGCC CTGGAAAAAG CGGAACAGCT CTGCAACCAG 
ATCAACGAAG CGGTCAAGGA GCGGGAGAAC TCGGACCACC TGGAGTGGGC GCAGACGCAT 
ATCCAGTGCA ACGGCATCTC GGAGAAACTC GTGTTCAATT CAACCACCAA CGTCCTCGGC 
TCCCGAAAGT TTTTACACTC GGGTTCTCTC ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG 
GGATTCCTTT TCAACGACTT TCTGCTGCTC GCGCAACCCG TTCGAGACCT GGGGAGAGTG 
ACCAACGTAT TCATGTCGGA TCGAGCCATG AACACCGCCT ACAGGTTATA CAAGCAGCCT 
CTATTCCTGA ACACGGTGAT GGTGCAGGAC ACCTCGGACG GAAGTCCCAC GGACGACACG 
TTCCAGATCT CGGCTCCCGA TTACATCTGC GTGTTCCGAG CGGCCAACCC AAACGAAAAG 
TTGCTTTGGG TGAAGAAGAT TAAAGACGCC GCGCACAACT ACCGGAACAC GGAAGCCAAG 
CAGATGAAGG TTTCGGCCAA GATGCAGAAA CAGAGCGGTG TGGGCCGCTT GCTAGTTGTG 
GTTGTAGAAG GACGTAACCT GAGGAACGGG TCTCCCCAGG GGCAGATCGA TTCGTACTGC 
GAGGTGAACA TGGCCTCCCA GGAGCACAAG ACGCACGTGG TGTCGAGTAC CACGAGCCCC 
CAGTGGAACA CGTCCATGCA GTTCCTGGTC AGGGACCTCC GGCAAGACGT CCTCTGCGTC 
ACCGTCTTCA ACAAGGAGCT CTTTTCGCCC AACGACTTCT TGGGCCGCAC CGAGGTCCGG 
GTGTGGGACA TCGCCCAGGA GACGCGAGAG AGCCACGGGC CAATCACCAA GCAGCTCCGG 
CTCTACGAGG TGGACCAAGG AGAAGTGCTC ATCAGGCTGG ACCTGCAGAT CTTCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002408358.1
CDS	1..1800
Translation	MKLTKKPEIASVIAPYKATGPEQLTLEKGQLIQVRKKTEEQVRALFPFVAQHEDELSFQKGQVVTVLSKEDPSWWKGELGGHVGLFPSNYVEPLDRADKCKCPLPDILFSSSLSTREKKRQGHIYELLSTEENYLKDLTLVKEVFYRPMKQSTLLTDDEVKLIFVNWPELIMCNTKMLKSFRVRQRMSENGIIEMIGDILCENLPYLNPYVRFCSCQVRSMAFIQQKSESDPRFKSFTRDFCKNPKTRGMPFTSFLLKPMQRMTKYPLMIKKIREYTDPTHHDRGYLDEALEKAEQLCNQINEAVKERENSDHLEWAQTHIQCNGISEKLVFNSTTNVLGSRKFLHSGSLTKVKSGKELVGFLFNDFLLLAQPVRDLGRVTNVFMSDRAMNTAYRLYKQPLFLNTVMVQDTSDGSPTDDTFQISAPDYICVFRAANPNEKLLWVKKIKDAAHNYRNTEAKQMKVSAKMQKQSGVGRLLVVVVEGRNLRNGSPQGQIDSYCEVNMASQEHKTHVVSSTTSPQWNTSMQFLVRDLRQDVLCVTVFNKELFSPNDFLGRTEVRVWDIAQETRESHGPITKQLRLYEVDQGEVLIRLDLQIFE

Target ORF information:

RefSeq Version	XM_002408314.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis Rho/RAC guanine nucleotide exchange factor, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002408314.1

ORF Insert Sequence:

ATGAAGCTGA CTAAGAAGCC AGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGAG 
CAAGTGCGTG CCCTGTTCCC ATTTGTGGCC CAACACGAGG ACGAGCTGTC ATTCCAGAAG 
GGTCAGGTGG TGACGGTGCT GAGCAAGGAG GACCCCTCTT GGTGGAAGGG GGAGCTGGGC 
GGACACGTGG GACTCTTCCC TTCCAACTAC GTCGAGCCAC TGGATCGGGC TGACAAGTGT 
AAGTGCCCCC TCCCTGACAT TTTGTTCAGC TCCTCCCTGA GCACGCGCGA GAAGAAGAGG 
CAAGGCCACA TCTACGAGCT CCTGTCCACG GAAGAGAACT ACCTGAAGGA CCTGACCCTA 
GTCAAGGAGG TTTTCTACAG GCCAATGAAA CAGTCCACTC TTCTAACGGA CGACGAGGTT 
AAACTGATCT TCGTAAACTG GCCAGAGCTC ATCATGTGCA ACACCAAGAT GCTCAAATCG 
TTTCGGGTAC GACAGCGAAT GAGCGAGAAC GGCATCATAG AGATGATTGG AGACATCCTT 
TGTGAAAATC TACCGTACCT TAACCCGTAC GTGAGGTTCT GCAGCTGCCA GGTCCGATCC 
ATGGCCTTCA TTCAACAGAA ATCCGAAAGC GATCCCCGCT TCAAATCCTT CACCAGGGAT 
TTCTGCAAGA ACCCGAAGAC CCGCGGCATG CCTTTCACCA GCTTCCTTCT GAAGCCAATG 
CAGAGGATGA CCAAGTACCC CCTCATGATT AAAAAGATTC GCGAGTACAC GGACCCGACG 
CACCACGACC GCGGCTACCT GGACGAGGCC CTGGAAAAAG CGGAACAGCT CTGCAACCAG 
ATCAACGAAG CGGTCAAGGA GCGGGAGAAC TCGGACCACC TGGAGTGGGC GCAGACGCAT 
ATCCAGTGCA ACGGCATCTC GGAGAAACTC GTGTTCAATT CAACCACCAA CGTCCTCGGC 
TCCCGAAAGT TTTTACACTC GGGTTCTCTC ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG 
GGATTCCTTT TCAACGACTT TCTGCTGCTC GCGCAACCCG TTCGAGACCT GGGGAGAGTG 
ACCAACGTAT TCATGTCGGA TCGAGCCATG AACACCGCCT ACAGGTTATA CAAGCAGCCT 
CTATTCCTGA ACACGGTGAT GGTGCAGGAC ACCTCGGACG GAAGTCCCAC GGACGACACG 
TTCCAGATCT CGGCTCCCGA TTACATCTGC GTGTTCCGAG CGGCCAACCC AAACGAAAAG 
TTGCTTTGGG TGAAGAAGAT TAAAGACGCC GCGCACAACT ACCGGAACAC GGAAGCCAAG 
CAGATGAAGG TTTCGGCCAA GATGCAGAAA CAGAGCGGTG TGGGCCGCTT GCTAGTTGTG 
GTTGTAGAAG GACGTAACCT GAGGAACGGG TCTCCCCAGG GGCAGATCGA TTCGTACTGC 
GAGGTGAACA TGGCCTCCCA GGAGCACAAG ACGCACGTGG TGTCGAGTAC CACGAGCCCC 
CAGTGGAACA CGTCCATGCA GTTCCTGGTC AGGGACCTCC GGCAAGACGT CCTCTGCGTC 
ACCGTCTTCA ACAAGGAGCT CTTTTCGCCC AACGACTTCT TGGGCCGCAC CGAGGTCCGG 
GTGTGGGACA TCGCCCAGGA GACGCGAGAG AGCCACGGGC CAATCACCAA GCAGCTCCGG 
CTCTACGAGG TGGACCAAGG AGAAGTGCTC ATCAGGCTGG ACCTGCAGAT CTTCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8032044 ( XM_029987659.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843519.1 Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa29638
Clone ID Related Accession (Same CDS sequence)	XM_029987659.1 , XM_029987660.1
Accession Version	XM_029987659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920985.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGCCAA GATGCAGAAA 
CAGAGCGGTG TGGGCCGCTT GCTAGTTGTG GTTGTAGAAG GACGTAACCT GAGGAACGGG 
TCTCCCCAGG GGCAGATCGA TTCGTACTGC GAGGTGAACA TGGCCTCCCA GGAGCACAAG 
ACGCACGTGG TGTCGAGCAC CACCAGCCCC CAGTGGAACA CGTCCATGCA GTTCCTGGTC 
AGGGACCTCC GGCAAGACGT CCTCTGCGTC ACCGTCTTCA ACAAGGAGCT CTTTTCGCCC 
AACGACTTCT TGGGCCGCAC CGAGGTCCGG GTGTGGGACA TCGCGCAGGA GACGCGAGAG 
AGCCACGGGC CAATCACCAA GCAGCTCCGG CTCTACGAGG TGGACCAAGG AGAAGTGCTC 
ATCAGGCTGG ACCTGCAGAT CTTCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843519.1
CDS	183..5072
Translation	MAGFDPWTIPPESRAKFDAQFLQMQPVGGLITGEQAKKLFLQSGLPPAVLAKVWSLADMDADGKINRHEFAVALHLIQMKLKGLELPAMLPSSLRSPPFPTGAFSPTFGPPLEQVVSPVAQPLVNAALPGAAFPVASPLPPPSMVGPHVAPLGIIPGAVGAGKPYTPGSATPPALTEWAVPQPSKLKYTQLFNSHDRSRSGFLGGGQARTILLQSALPHTVLAQIWNLSDIDSDGRLTCEEFVLAMHLVDCVRAGDTLPAKLPLDLIPPSYRRQRSDSVQSTGSSGTELSGPAGEARAEEEKLRSLATFEDKRRANFEKGQAELEKRRQALLESQRKEQQERERKDREEQEKRERIRQEQERRRQLELEKQLARQRELEQEKEEQRRKALEQREAARREMERQRQLEWEKQRMQELLSQKQREQENMSRLKAHKTSIFLQLEQMENKINESNLKLADTKKGVAEMKACIDEMRLERDAKMKELNETKLELKEISDKMLLLNQEKLSHQGRLKMAAGGSNNTYSLAMNNYGSKEISLNMLRTTLSNIEKETETKQQDVNNNNAQLKELKEKLSELVQQNELLLQEFTQKKQQVMALKKQKGNVASVDAWNDQSEFAPVDSWPGDGVQQTTTSTGLVKYRALYAFEARNEDELSIMPGDIVMVQENQHGEPGWLGGELRGKTGWFPESYVEKLVSTPPGAFAEVPGEAVATAADVLTAAETVHIVGTEMKRTLEGISEAPENGREHAVELAAAPDVLQPSENSCFTQLPRVPPMDTTSPVPGEGQAAPQGLQAQALFPWRAKKENHLSFNKGDVITVKEQQDMWWYGDFEGKVGWFPKSYVRLVSGPMKSFNNNQISDSQEFGDVPPEPPSDFPVVSERYVAVYPYQSQEPGDLSFLAGDVLIVNKKEGEWWTGTIGGMTGIFPSNYVRPCEPEANNKGKKNSISEDFIPSATTLPTNEEVGLSGQEPSRPDTPQSDAKAKNMKLTKKPEIASVIAPYKATGPEQLTLEKGQLIQVRKKTEGGWWEGELQVKGKKRQSGWFPASYVKVLGGSGASSSRSSPVPTAFASSTQGEQVRALFPFVAQHEDELSFQKGQVVTVLSKEDPSWWKGELGGHVGLFPSNYVEPLDRADKCSGLQDILFSSSLSTREKKRQGHIYELLSTEENYLKDLTLVKEVFYRPMKQSTLLTDDEVKLIFVNWPELIMCNTKMLKSFRVRQRMSENGIIEMIGDILCENLPYLNPYVRFCSCQVRSMAFIQQKSESDPRFKSFTRDFCKNPKTRGMPFTSFLLKPMQRMTKYPLMIKKIREYTDPTHHDRGYLDEALEKAEQLCNQINEAVKERENSDHLEWAQTHIQCNGISEKLVFNSTTNVLGSRKFLHSGSLTKVKSGKELVGFLFNDFLLLSQPVRDLGRVTNVFMSDRAMNTAYRLYKQPLFLNTVMVQDTSDGSPTDDTFQISAPDYICVFRAANPNEKLLWVKKIKDAAHNYRNTEAKQMKVSAKMQKQSGVGRLLVVVVEGRNLRNGSPQGQIDSYCEVNMASQEHKTHVVSSTTSPQWNTSMQFLVRDLRQDVLCVTVFNKELFSPNDFLGRTEVRVWDIAQETRESHGPITKQLRLYEVDQGEVLIRLDLQIFE

Target ORF information:

RefSeq Version	XM_029987659.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987659.1

ORF Insert Sequence:

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGCCAA GATGCAGAAA 
CAGAGCGGTG TGGGCCGCTT GCTAGTTGTG GTTGTAGAAG GACGTAACCT GAGGAACGGG 
TCTCCCCAGG GGCAGATCGA TTCGTACTGC GAGGTGAACA TGGCCTCCCA GGAGCACAAG 
ACGCACGTGG TGTCGAGCAC CACCAGCCCC CAGTGGAACA CGTCCATGCA GTTCCTGGTC 
AGGGACCTCC GGCAAGACGT CCTCTGCGTC ACCGTCTTCA ACAAGGAGCT CTTTTCGCCC 
AACGACTTCT TGGGCCGCAC CGAGGTCCGG GTGTGGGACA TCGCGCAGGA GACGCGAGAG 
AGCCACGGGC CAATCACCAA GCAGCTCCGG CTCTACGAGG TGGACCAAGG AGAAGTGCTC 
ATCAGGCTGG ACCTGCAGAT CTTCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8032044 ( XM_029987660.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843520.1 Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X2, mRNA.

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CloneID	OIa29638
Clone ID Related Accession (Same CDS sequence)	XM_029987659.1 , XM_029987660.1
Accession Version	XM_029987660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920985.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGCCAA GATGCAGAAA 
CAGAGCGGTG TGGGCCGCTT GCTAGTTGTG GTTGTAGAAG GACGTAACCT GAGGAACGGG 
TCTCCCCAGG GGCAGATCGA TTCGTACTGC GAGGTGAACA TGGCCTCCCA GGAGCACAAG 
ACGCACGTGG TGTCGAGCAC CACCAGCCCC CAGTGGAACA CGTCCATGCA GTTCCTGGTC 
AGGGACCTCC GGCAAGACGT CCTCTGCGTC ACCGTCTTCA ACAAGGAGCT CTTTTCGCCC 
AACGACTTCT TGGGCCGCAC CGAGGTCCGG GTGTGGGACA TCGCGCAGGA GACGCGAGAG 
AGCCACGGGC CAATCACCAA GCAGCTCCGG CTCTACGAGG TGGACCAAGG AGAAGTGCTC 
ATCAGGCTGG ACCTGCAGAT CTTCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843520.1
CDS	135..5024
Translation	MAGFDPWTIPPESRAKFDAQFLQMQPVGGLITGEQAKKLFLQSGLPPAVLAKVWSLADMDADGKINRHEFAVALHLIQMKLKGLELPAMLPSSLRSPPFPTGAFSPTFGPPLEQVVSPVAQPLVNAALPGAAFPVASPLPPPSMVGPHVAPLGIIPGAVGAGKPYTPGSATPPALTEWAVPQPSKLKYTQLFNSHDRSRSGFLGGGQARTILLQSALPHTVLAQIWNLSDIDSDGRLTCEEFVLAMHLVDCVRAGDTLPAKLPLDLIPPSYRRQRSDSVQSTGSSGTELSGPAGEARAEEEKLRSLATFEDKRRANFEKGQAELEKRRQALLESQRKEQQERERKDREEQEKRERIRQEQERRRQLELEKQLARQRELEQEKEEQRRKALEQREAARREMERQRQLEWEKQRMQELLSQKQREQENMSRLKAHKTSIFLQLEQMENKINESNLKLADTKKGVAEMKACIDEMRLERDAKMKELNETKLELKEISDKMLLLNQEKLSHQGRLKMAAGGSNNTYSLAMNNYGSKEISLNMLRTTLSNIEKETETKQQDVNNNNAQLKELKEKLSELVQQNELLLQEFTQKKQQVMALKKQKGNVASVDAWNDQSEFAPVDSWPGDGVQQTTTSTGLVKYRALYAFEARNEDELSIMPGDIVMVQENQHGEPGWLGGELRGKTGWFPESYVEKLVSTPPGAFAEVPGEAVATAADVLTAAETVHIVGTEMKRTLEGISEAPENGREHAVELAAAPDVLQPSENSCFTQLPRVPPMDTTSPVPGEGQAAPQGLQAQALFPWRAKKENHLSFNKGDVITVKEQQDMWWYGDFEGKVGWFPKSYVRLVSGPMKSFNNNQISDSQEFGDVPPEPPSDFPVVSERYVAVYPYQSQEPGDLSFLAGDVLIVNKKEGEWWTGTIGGMTGIFPSNYVRPCEPEANNKGKKNSISEDFIPSATTLPTNEEVGLSGQEPSRPDTPQSDAKAKNMKLTKKPEIASVIAPYKATGPEQLTLEKGQLIQVRKKTEGGWWEGELQVKGKKRQSGWFPASYVKVLGGSGASSSRSSPVPTAFASSTQGEQVRALFPFVAQHEDELSFQKGQVVTVLSKEDPSWWKGELGGHVGLFPSNYVEPLDRADKCSGLQDILFSSSLSTREKKRQGHIYELLSTEENYLKDLTLVKEVFYRPMKQSTLLTDDEVKLIFVNWPELIMCNTKMLKSFRVRQRMSENGIIEMIGDILCENLPYLNPYVRFCSCQVRSMAFIQQKSESDPRFKSFTRDFCKNPKTRGMPFTSFLLKPMQRMTKYPLMIKKIREYTDPTHHDRGYLDEALEKAEQLCNQINEAVKERENSDHLEWAQTHIQCNGISEKLVFNSTTNVLGSRKFLHSGSLTKVKSGKELVGFLFNDFLLLSQPVRDLGRVTNVFMSDRAMNTAYRLYKQPLFLNTVMVQDTSDGSPTDDTFQISAPDYICVFRAANPNEKLLWVKKIKDAAHNYRNTEAKQMKVSAKMQKQSGVGRLLVVVVEGRNLRNGSPQGQIDSYCEVNMASQEHKTHVVSSTTSPQWNTSMQFLVRDLRQDVLCVTVFNKELFSPNDFLGRTEVRVWDIAQETRESHGPITKQLRLYEVDQGEVLIRLDLQIFE

Target ORF information:

RefSeq Version	XM_029987660.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987660.1

ORF Insert Sequence:

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGCCAA GATGCAGAAA 
CAGAGCGGTG TGGGCCGCTT GCTAGTTGTG GTTGTAGAAG GACGTAACCT GAGGAACGGG 
TCTCCCCAGG GGCAGATCGA TTCGTACTGC GAGGTGAACA TGGCCTCCCA GGAGCACAAG 
ACGCACGTGG TGTCGAGCAC CACCAGCCCC CAGTGGAACA CGTCCATGCA GTTCCTGGTC 
AGGGACCTCC GGCAAGACGT CCTCTGCGTC ACCGTCTTCA ACAAGGAGCT CTTTTCGCCC 
AACGACTTCT TGGGCCGCAC CGAGGTCCGG GTGTGGGACA TCGCGCAGGA GACGCGAGAG 
AGCCACGGGC CAATCACCAA GCAGCTCCGG CTCTACGAGG TGGACCAAGG AGAAGTGCTC 
ATCAGGCTGG ACCTGCAGAT CTTCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8032044 ( XM_029987661.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843521.1 Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X3, mRNA.

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CloneID	OIa29639
Clone ID Related Accession (Same CDS sequence)	XM_029987661.1
Accession Version	XM_029987661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920985.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGTGCA CCGCAGACCT 
GCGGAGGGAA AACTAAGGGT AGTTGTAATG CAAGCATGCG ATCTCGTCTC GATGGACCGA 
CCAGGGCAGA TCGATTCGTA CTGCGAGGTG AACATGGCCT CCCAGGAGCA CAAGACGCAC 
GTGGTGTCGA GCACCACCAG CCCCCAGTGG AACACGTCCA TGCAGTTCCT GGTCAGGGAC 
CTCCGGCAAG ACGTCCTCTG CGTCACCGTC TTCAACAAGG AGCTCTTTTC GCCCAACGAC 
TTCTTGGGCC GCACCGAGGT CCGGGTGTGG GACATCGCGC AGGAGACGCG AGAGAGCCAC 
GGGCCAATCA CCAAGCAGCT CCGGCTCTAC GAGGTGGACC AAGGAGAAGT GCTCATCAGG 
CTGGACCTGC AGATCTTCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843521.1
CDS	183..5066
Translation	MAGFDPWTIPPESRAKFDAQFLQMQPVGGLITGEQAKKLFLQSGLPPAVLAKVWSLADMDADGKINRHEFAVALHLIQMKLKGLELPAMLPSSLRSPPFPTGAFSPTFGPPLEQVVSPVAQPLVNAALPGAAFPVASPLPPPSMVGPHVAPLGIIPGAVGAGKPYTPGSATPPALTEWAVPQPSKLKYTQLFNSHDRSRSGFLGGGQARTILLQSALPHTVLAQIWNLSDIDSDGRLTCEEFVLAMHLVDCVRAGDTLPAKLPLDLIPPSYRRQRSDSVQSTGSSGTELSGPAGEARAEEEKLRSLATFEDKRRANFEKGQAELEKRRQALLESQRKEQQERERKDREEQEKRERIRQEQERRRQLELEKQLARQRELEQEKEEQRRKALEQREAARREMERQRQLEWEKQRMQELLSQKQREQENMSRLKAHKTSIFLQLEQMENKINESNLKLADTKKGVAEMKACIDEMRLERDAKMKELNETKLELKEISDKMLLLNQEKLSHQGRLKMAAGGSNNTYSLAMNNYGSKEISLNMLRTTLSNIEKETETKQQDVNNNNAQLKELKEKLSELVQQNELLLQEFTQKKQQVMALKKQKGNVASVDAWNDQSEFAPVDSWPGDGVQQTTTSTGLVKYRALYAFEARNEDELSIMPGDIVMVQENQHGEPGWLGGELRGKTGWFPESYVEKLVSTPPGAFAEVPGEAVATAADVLTAAETVHIVGTEMKRTLEGISEAPENGREHAVELAAAPDVLQPSENSCFTQLPRVPPMDTTSPVPGEGQAAPQGLQAQALFPWRAKKENHLSFNKGDVITVKEQQDMWWYGDFEGKVGWFPKSYVRLVSGPMKSFNNNQISDSQEFGDVPPEPPSDFPVVSERYVAVYPYQSQEPGDLSFLAGDVLIVNKKEGEWWTGTIGGMTGIFPSNYVRPCEPEANNKGKKNSISEDFIPSATTLPTNEEVGLSGQEPSRPDTPQSDAKAKNMKLTKKPEIASVIAPYKATGPEQLTLEKGQLIQVRKKTEGGWWEGELQVKGKKRQSGWFPASYVKVLGGSGASSSRSSPVPTAFASSTQGEQVRALFPFVAQHEDELSFQKGQVVTVLSKEDPSWWKGELGGHVGLFPSNYVEPLDRADKCSGLQDILFSSSLSTREKKRQGHIYELLSTEENYLKDLTLVKEVFYRPMKQSTLLTDDEVKLIFVNWPELIMCNTKMLKSFRVRQRMSENGIIEMIGDILCENLPYLNPYVRFCSCQVRSMAFIQQKSESDPRFKSFTRDFCKNPKTRGMPFTSFLLKPMQRMTKYPLMIKKIREYTDPTHHDRGYLDEALEKAEQLCNQINEAVKERENSDHLEWAQTHIQCNGISEKLVFNSTTNVLGSRKFLHSGSLTKVKSGKELVGFLFNDFLLLSQPVRDLGRVTNVFMSDRAMNTAYRLYKQPLFLNTVMVQDTSDGSPTDDTFQISAPDYICVFRAANPNEKLLWVKKIKDAAHNYRNTEAKQMKVSVHRRPAEGKLRVVVMQACDLVSMDRPGQIDSYCEVNMASQEHKTHVVSSTTSPQWNTSMQFLVRDLRQDVLCVTVFNKELFSPNDFLGRTEVRVWDIAQETRESHGPITKQLRLYEVDQGEVLIRLDLQIFE

Target ORF information:

RefSeq Version	XM_029987661.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987661.1

ORF Insert Sequence:

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGTGCA CCGCAGACCT 
GCGGAGGGAA AACTAAGGGT AGTTGTAATG CAAGCATGCG ATCTCGTCTC GATGGACCGA 
CCAGGGCAGA TCGATTCGTA CTGCGAGGTG AACATGGCCT CCCAGGAGCA CAAGACGCAC 
GTGGTGTCGA GCACCACCAG CCCCCAGTGG AACACGTCCA TGCAGTTCCT GGTCAGGGAC 
CTCCGGCAAG ACGTCCTCTG CGTCACCGTC TTCAACAAGG AGCTCTTTTC GCCCAACGAC 
TTCTTGGGCC GCACCGAGGT CCGGGTGTGG GACATCGCGC AGGAGACGCG AGAGAGCCAC 
GGGCCAATCA CCAAGCAGCT CCGGCTCTAC GAGGTGGACC AAGGAGAAGT GCTCATCAGG 
CTGGACCTGC AGATCTTCGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8032044 ( XM_029987662.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843522.1 Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OIa29640
Clone ID Related Accession (Same CDS sequence)	XM_029987662.1
Accession Version	XM_029987662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920985.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGAACAT GAAGCTGACC AAGAAGCCGG AGATAGCGTC GGTGATTGCC 
CCTTACAAGG CGACCGGGCC GGAACAACTT ACCCTGGAGA AAGGGCAGCT CATCCAAGTG 
CGCAAAAAAA CGGAAGGTGG CTGGTGGGAA GGAGAACTAC AGGTCAAAGG AAAGAAGAGG 
CAGTCGGGAT GGTTCCCGGC ATCTTACGTG AAAGTCCTCG GAGGTAGCGG TGCAAGCAGC 
AGCAGAAGTT CGCCTGTGCC CACTGCATTC GCTTCCAGTA CTCAAGGGGA GCAAGTGCGT 
GCCCTGTTCC CATTTGTGGC CCAACACGAG GACGAGCTGT CATTCCAGAA GGGTCAGGTG 
GTGACGGTGC TGAGCAAGGA GGACCCCTCT TGGTGGAAAG GGGAGCTGGG CGGACACGTG 
GGACTCTTCC CTTCCAACTA CGTCGAGCCA CTGGATCGGG CTGACAAGTG TTCCGGGCTC 
CAGGACATTC TGTTCAGCTC CTCCCTGAGC ACGCGCGAGA AGAAGAGACA AGGCCACATC 
TACGAGCTCC TGTCCACGGA AGAGAACTAC CTGAAGGACC TGACCCTAGT CAAGGAGGTT 
TTTTACAGGC CAATGAAACA GTCCACTCTT CTAACGGACG ATGAGGTTAA ACTGATCTTC 
GTAAATTGGC CAGAGCTCAT CATGTGCAAC ACCAAGATGC TCAAATCGTT TCGAGTACGA 
CAGCGAATGA GCGAGAACGG CATCATAGAG ATGATTGGAG ACATCCTCTG TGAAAATCTA 
CCGTACCTTA ACCCGTACGT GAGGTTCTGC AGCTGCCAGG TCCGATCCAT GGCCTTCATT 
CAGCAGAAAT CCGAAAGCGA CCCCCGCTTC AAATCCTTCA CCAGGGATTT CTGCAAGAAC 
CCGAAGACCC GCGGCATGCC TTTCACCAGC TTCCTTCTGA AGCCAATGCA GAGGATGACC 
AAGTACCCCC TGATGATTAA AAAGATTCGC GAGTACACGG ACCCGACGCA CCACGACCGC 
GGCTACCTGG ACGAGGCCCT GGAGAAAGCG GAACAGCTCT GCAACCAGAT CAACGAAGCG 
GTCAAGGAGC GGGAGAACTC GGACCACCTG GAGTGGGCGC AGACGCATAT CCAGTGCAAC 
GGCATCTCGG AGAAACTCGT GTTCAATTCA ACCACCAACG TCCTCGGCTC CCGAAAGTTT 
TTGCACTCGG GTTCTCTCAC GAAAGTCAAA AGCGGGAAGG AGTTGGTGGG ATTCCTTTTC 
AACGACTTTC TGCTGCTCTC GCAACCCGTT CGAGACCTGG GGAGAGTGAC CAACGTATTC 
ATGTCGGATC GAGCCATGAA CACTGCCTAC AGGTTATACA AGCAGCCTCT ATTCCTGAAC 
ACGGTGATGG TGCAGGACAC CTCGGACGGA AGTCCCACGG ACGACACGTT CCAGATCTCG 
GCTCCCGATT ACATCTGCGT GTTCCGAGCG GCCAACCCAA ACGAAAAGTT GCTTTGGGTG 
AAGAAGATTA AAGACGCCGC GCACAACTAC CGGAACACGG AAGCCAAGCA GATGAAGGTT 
TCGGCCAAGA TGCAGAAACA GAGCGGTGTG GGCCGCTTGC TAGTTGTGGT TGTAGAAGGA 
CGTAACCTGA GGAACGGGTC TCCCCAGGGG CAGATCGATT CGTACTGCGA GGTGAACATG 
GCCTCCCAGG AGCACAAGAC GCACGTGGTG TCGAGCACCA CCAGCCCCCA GTGGAACACG 
TCCATGCAGT TCCTGGTCAG GGACCTCCGG CAAGACGTCC TCTGCGTCAC CGTCTTCAAC 
AAGGAGCTCT TTTCGCCCAA CGACTTCTTG GGCCGCACCG AGGTCCGGGT GTGGGACATC 
GCGCAGGAGA CGCGAGAGAG CCACGGGCCA ATCACCAAGC AGCTCCGGCT CTACGAGGTG 
GACCAAGGAG AAGTGCTCAT CAGGCTGGAC CTGCAGATCT TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843522.1
CDS	183..5030
Translation	MAGFDPWTIPPESRAKFDAQFLQMQPVGGLITGEQAKKLFLQSGLPPAVLAKVWSLADMDADGKINRHEFAVALHLIQMKLKGLELPAMLPSSLRSPPFPTGAFSPTFGPPLEQVVSPVAQPLVNAALPGAAFPVASPLPPPSMVGPHVAPLGIIPGAVGAGKPYTPGSATPPALTEWAVPQPSKLKYTQLFNSHDRSRSGFLGGGQARTILLQSALPHTVLAQIWNLSDIDSDGRLTCEEFVLAMHLVDCVRAGDTLPAKLPLDLIPPSYRRQRSDSVQSTGSSGTELSGPAGEARAEEEKLRSLATFEDKRRANFEKGQAELEKRRQALLESQRKEQQERERKDREEQEKRERIRQEQERRRQLELEKQLARQRELEQEKEEQRRKALEQREAARREMERQRQLEWEKQRMQELLSQKQREQENMSRLKAHKTSIFLQLEQMENKINESNLKLADTKKGVAEMKACIDEMRLERDAKMKELNETKLELKEISDKMLLLNQEKLSHQGRLKMAAGGSNNTYSLAMNNYGSKEISLNMLRTTLSNIEKETETKQQDVNNNNAQLKELKEKLSELVQQNELLLQEFTQKKQQVMALKKQKGNVASVDAWNDQSEFAPVDSWPGDGVQQTTTSTGLVKYRALYAFEARNEDELSIMPGDIVMVQENQHGEPGWLGGELRGKTGWFPESYVEKLVSTPPGAFAEVPGEAVATAADVLTAAETVHIVGTEMKRTLEGISEAPENGREHAVELAAAPDVLQPSENSCFTQLPRVPPMDTTSPVPGEGQAAPQGLQAQALFPWRAKKENHLSFNKGDVITVKEQQDMWWYGDFEGKVGWFPKSYVRLVSGPMKSFNNNQISDSQEFGDVPPEPPSDFPVVSERYVAVYPYQSQEPGDLSFLAGDVLIVNKKEGEWWTGTIGGMTGIFPSNYVRPCEPEANNKGKKNSISEDFIPSATTLPTNEEVGLSGQENMKLTKKPEIASVIAPYKATGPEQLTLEKGQLIQVRKKTEGGWWEGELQVKGKKRQSGWFPASYVKVLGGSGASSSRSSPVPTAFASSTQGEQVRALFPFVAQHEDELSFQKGQVVTVLSKEDPSWWKGELGGHVGLFPSNYVEPLDRADKCSGLQDILFSSSLSTREKKRQGHIYELLSTEENYLKDLTLVKEVFYRPMKQSTLLTDDEVKLIFVNWPELIMCNTKMLKSFRVRQRMSENGIIEMIGDILCENLPYLNPYVRFCSCQVRSMAFIQQKSESDPRFKSFTRDFCKNPKTRGMPFTSFLLKPMQRMTKYPLMIKKIREYTDPTHHDRGYLDEALEKAEQLCNQINEAVKERENSDHLEWAQTHIQCNGISEKLVFNSTTNVLGSRKFLHSGSLTKVKSGKELVGFLFNDFLLLSQPVRDLGRVTNVFMSDRAMNTAYRLYKQPLFLNTVMVQDTSDGSPTDDTFQISAPDYICVFRAANPNEKLLWVKKIKDAAHNYRNTEAKQMKVSAKMQKQSGVGRLLVVVVEGRNLRNGSPQGQIDSYCEVNMASQEHKTHVVSSTTSPQWNTSMQFLVRDLRQDVLCVTVFNKELFSPNDFLGRTEVRVWDIAQETRESHGPITKQLRLYEVDQGEVLIRLDLQIFE

Target ORF information:

RefSeq Version	XM_029987662.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987662.1

ORF Insert Sequence:

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGAACAT GAAGCTGACC AAGAAGCCGG AGATAGCGTC GGTGATTGCC 
CCTTACAAGG CGACCGGGCC GGAACAACTT ACCCTGGAGA AAGGGCAGCT CATCCAAGTG 
CGCAAAAAAA CGGAAGGTGG CTGGTGGGAA GGAGAACTAC AGGTCAAAGG AAAGAAGAGG 
CAGTCGGGAT GGTTCCCGGC ATCTTACGTG AAAGTCCTCG GAGGTAGCGG TGCAAGCAGC 
AGCAGAAGTT CGCCTGTGCC CACTGCATTC GCTTCCAGTA CTCAAGGGGA GCAAGTGCGT 
GCCCTGTTCC CATTTGTGGC CCAACACGAG GACGAGCTGT CATTCCAGAA GGGTCAGGTG 
GTGACGGTGC TGAGCAAGGA GGACCCCTCT TGGTGGAAAG GGGAGCTGGG CGGACACGTG 
GGACTCTTCC CTTCCAACTA CGTCGAGCCA CTGGATCGGG CTGACAAGTG TTCCGGGCTC 
CAGGACATTC TGTTCAGCTC CTCCCTGAGC ACGCGCGAGA AGAAGAGACA AGGCCACATC 
TACGAGCTCC TGTCCACGGA AGAGAACTAC CTGAAGGACC TGACCCTAGT CAAGGAGGTT 
TTTTACAGGC CAATGAAACA GTCCACTCTT CTAACGGACG ATGAGGTTAA ACTGATCTTC 
GTAAATTGGC CAGAGCTCAT CATGTGCAAC ACCAAGATGC TCAAATCGTT TCGAGTACGA 
CAGCGAATGA GCGAGAACGG CATCATAGAG ATGATTGGAG ACATCCTCTG TGAAAATCTA 
CCGTACCTTA ACCCGTACGT GAGGTTCTGC AGCTGCCAGG TCCGATCCAT GGCCTTCATT 
CAGCAGAAAT CCGAAAGCGA CCCCCGCTTC AAATCCTTCA CCAGGGATTT CTGCAAGAAC 
CCGAAGACCC GCGGCATGCC TTTCACCAGC TTCCTTCTGA AGCCAATGCA GAGGATGACC 
AAGTACCCCC TGATGATTAA AAAGATTCGC GAGTACACGG ACCCGACGCA CCACGACCGC 
GGCTACCTGG ACGAGGCCCT GGAGAAAGCG GAACAGCTCT GCAACCAGAT CAACGAAGCG 
GTCAAGGAGC GGGAGAACTC GGACCACCTG GAGTGGGCGC AGACGCATAT CCAGTGCAAC 
GGCATCTCGG AGAAACTCGT GTTCAATTCA ACCACCAACG TCCTCGGCTC CCGAAAGTTT 
TTGCACTCGG GTTCTCTCAC GAAAGTCAAA AGCGGGAAGG AGTTGGTGGG ATTCCTTTTC 
AACGACTTTC TGCTGCTCTC GCAACCCGTT CGAGACCTGG GGAGAGTGAC CAACGTATTC 
ATGTCGGATC GAGCCATGAA CACTGCCTAC AGGTTATACA AGCAGCCTCT ATTCCTGAAC 
ACGGTGATGG TGCAGGACAC CTCGGACGGA AGTCCCACGG ACGACACGTT CCAGATCTCG 
GCTCCCGATT ACATCTGCGT GTTCCGAGCG GCCAACCCAA ACGAAAAGTT GCTTTGGGTG 
AAGAAGATTA AAGACGCCGC GCACAACTAC CGGAACACGG AAGCCAAGCA GATGAAGGTT 
TCGGCCAAGA TGCAGAAACA GAGCGGTGTG GGCCGCTTGC TAGTTGTGGT TGTAGAAGGA 
CGTAACCTGA GGAACGGGTC TCCCCAGGGG CAGATCGATT CGTACTGCGA GGTGAACATG 
GCCTCCCAGG AGCACAAGAC GCACGTGGTG TCGAGCACCA CCAGCCCCCA GTGGAACACG 
TCCATGCAGT TCCTGGTCAG GGACCTCCGG CAAGACGTCC TCTGCGTCAC CGTCTTCAAC 
AAGGAGCTCT TTTCGCCCAA CGACTTCTTG GGCCGCACCG AGGTCCGGGT GTGGGACATC 
GCGCAGGAGA CGCGAGAGAG CCACGGGCCA ATCACCAAGC AGCTCCGGCT CTACGAGGTG 
GACCAAGGAG AAGTGCTCAT CAGGCTGGAC CTGCAGATCT TCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8032044 ( XM_029987663.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843523.1 Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X5, mRNA.

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CloneID	OIa29641
Clone ID Related Accession (Same CDS sequence)	XM_029987663.1
Accession Version	XM_029987663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-29
Organism	Ixodes scapularis(black-legged tick)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021920985.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGGGCA GATCGATTCG 
TACTGCGAGG TGAACATGGC CTCCCAGGAG CACAAGACGC ACGTGGTGTC GAGCACCACC 
AGCCCCCAGT GGAACACGTC CATGCAGTTC CTGGTCAGGG ACCTCCGGCA AGACGTCCTC 
TGCGTCACCG TCTTCAACAA GGAGCTCTTT TCGCCCAACG ACTTCTTGGG CCGCACCGAG 
GTCCGGGTGT GGGACATCGC GCAGGAGACG CGAGAGAGCC ACGGGCCAAT CACCAAGCAG 
CTCCGGCTCT ACGAGGTGGA CCAAGGAGAA GTGCTCATCA GGCTGGACCT GCAGATCTTC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843523.1
CDS	183..4988
Translation	MAGFDPWTIPPESRAKFDAQFLQMQPVGGLITGEQAKKLFLQSGLPPAVLAKVWSLADMDADGKINRHEFAVALHLIQMKLKGLELPAMLPSSLRSPPFPTGAFSPTFGPPLEQVVSPVAQPLVNAALPGAAFPVASPLPPPSMVGPHVAPLGIIPGAVGAGKPYTPGSATPPALTEWAVPQPSKLKYTQLFNSHDRSRSGFLGGGQARTILLQSALPHTVLAQIWNLSDIDSDGRLTCEEFVLAMHLVDCVRAGDTLPAKLPLDLIPPSYRRQRSDSVQSTGSSGTELSGPAGEARAEEEKLRSLATFEDKRRANFEKGQAELEKRRQALLESQRKEQQERERKDREEQEKRERIRQEQERRRQLELEKQLARQRELEQEKEEQRRKALEQREAARREMERQRQLEWEKQRMQELLSQKQREQENMSRLKAHKTSIFLQLEQMENKINESNLKLADTKKGVAEMKACIDEMRLERDAKMKELNETKLELKEISDKMLLLNQEKLSHQGRLKMAAGGSNNTYSLAMNNYGSKEISLNMLRTTLSNIEKETETKQQDVNNNNAQLKELKEKLSELVQQNELLLQEFTQKKQQVMALKKQKGNVASVDAWNDQSEFAPVDSWPGDGVQQTTTSTGLVKYRALYAFEARNEDELSIMPGDIVMVQENQHGEPGWLGGELRGKTGWFPESYVEKLVSTPPGAFAEVPGEAVATAADVLTAAETVHIVGTEMKRTLEGISEAPENGREHAVELAAAPDVLQPSENSCFTQLPRVPPMDTTSPVPGEGQAAPQGLQAQALFPWRAKKENHLSFNKGDVITVKEQQDMWWYGDFEGKVGWFPKSYVRLVSGPMKSFNNNQISDSQEFGDVPPEPPSDFPVVSERYVAVYPYQSQEPGDLSFLAGDVLIVNKKEGEWWTGTIGGMTGIFPSNYVRPCEPEANNKGKKNSISEDFIPSATTLPTNEEVGLSGQEPSRPDTPQSDAKAKNMKLTKKPEIASVIAPYKATGPEQLTLEKGQLIQVRKKTEGGWWEGELQVKGKKRQSGWFPASYVKVLGGSGASSSRSSPVPTAFASSTQGEQVRALFPFVAQHEDELSFQKGQVVTVLSKEDPSWWKGELGGHVGLFPSNYVEPLDRADKCSGLQDILFSSSLSTREKKRQGHIYELLSTEENYLKDLTLVKEVFYRPMKQSTLLTDDEVKLIFVNWPELIMCNTKMLKSFRVRQRMSENGIIEMIGDILCENLPYLNPYVRFCSCQVRSMAFIQQKSESDPRFKSFTRDFCKNPKTRGMPFTSFLLKPMQRMTKYPLMIKKIREYTDPTHHDRGYLDEALEKAEQLCNQINEAVKERENSDHLEWAQTHIQCNGISEKLVFNSTTNVLGSRKFLHSGSLTKVKSGKELVGFLFNDFLLLSQPVRDLGRVTNVFMSDRAMNTAYRLYKQPLFLNTVMVQDTSDGSPTDDTFQISAPDYICVFRAANPNEKLLWVKKIKDAAHNYRNTEAKQMKVSGQIDSYCEVNMASQEHKTHVVSSTTSPQWNTSMQFLVRDLRQDVLCVTVFNKELFSPNDFLGRTEVRVWDIAQETRESHGPITKQLRLYEVDQGEVLIRLDLQIFE

Target ORF information:

RefSeq Version	XM_029987663.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis intersectin-1 (LOC8032044), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987663.1

ORF Insert Sequence:

ATGGCGGGGT TCGACCCGTG GACGATACCA CCTGAATCCC GGGCAAAATT TGATGCCCAG 
TTTCTTCAGA TGCAGCCTGT AGGGGGGCTC ATCACAGGTG AACAGGCCAA GAAACTTTTC 
CTTCAGTCTG GATTGCCTCC TGCTGTCCTT GCAAAAGTCT GGTCCCTGGC AGACATGGAC 
GCGGATGGCA AGATCAACCG GCACGAGTTT GCAGTGGCCT TGCATTTGAT ACAGATGAAG 
CTGAAGGGCC TGGAGTTGCC CGCCATGTTG CCTTCTTCCT TGCGCTCTCC GCCTTTTCCC 
ACGGGGGCAT TCAGCCCCAC CTTCGGGCCC CCACTAGAAC AAGTGGTGTC CCCGGTGGCA 
CAGCCTCTAG TCAACGCAGC TCTCCCGGGG GCTGCCTTCC CCGTGGCATC GCCCCTGCCG 
CCTCCATCCA TGGTGGGGCC GCACGTTGCA CCGCTGGGCA TCATTCCAGG GGCCGTGGGG 
GCGGGAAAGC CCTACACCCC TGGGAGTGCG ACCCCTCCCG CCCTGACAGA GTGGGCGGTG 
CCGCAGCCTT CCAAGCTCAA GTACACGCAG CTGTTCAACT CTCACGACCG CAGCCGCAGC 
GGGTTCCTCG GCGGGGGTCA GGCCCGCACC ATCCTGCTCC AGTCGGCCCT GCCGCACACG 
GTCCTCGCCC AGATCTGGAA CCTTTCAGAC ATAGACTCTG ATGGCCGTCT GACCTGCGAG 
GAGTTTGTGC TGGCCATGCA CCTGGTGGAC TGTGTGAGGG CAGGGGACAC CCTTCCAGCC 
AAGCTGCCTC TGGACCTGAT TCCACCGTCG TACCGGCGCC AGCGCTCGGA CAGCGTGCAG 
TCCACAGGCT CGTCCGGCAC GGAGCTGTCT GGCCCGGCGG GGGAGGCCCG GGCGGAGGAG 
GAGAAGCTGC GCAGCCTGGC GACGTTTGAG GACAAGCGGC GTGCCAACTT TGAGAAGGGC 
CAGGCGGAGC TGGAGAAGCG CAGGCAGGCC CTGCTGGAGA GCCAGCGCAA GGAGCAGCAG 
GAGCGGGAGC GCAAGGACCG CGAGGAGCAG GAGAAGAGGG AGCGCATACG GCAGGAGCAG 
GAGAGGCGGC GTCAGCTGGA GCTTGAGAAG CAGCTGGCTC GGCAACGAGA GCTGGAGCAG 
GAGAAAGAGG AGCAGAGGAG GAAGGCGCTG GAGCAGAGGG AGGCGGCCCG TCGGGAAATG 
GAGCGCCAGC GCCAGCTGGA GTGGGAGAAG CAGCGGATGC AGGAGCTGCT GTCGCAGAAG 
CAGAGGGAGC AGGAGAACAT GAGCCGGCTC AAGGCCCACA AGACCAGCAT CTTCCTCCAG 
CTGGAGCAGA TGGAGAACAA GATCAACGAG TCCAACCTGA AGCTTGCCGA CACTAAAAAG 
GGCGTAGCTG AGATGAAGGC CTGCATCGAC GAGATGAGGC TGGAGAGGGA CGCCAAGATG 
AAGGAACTGA ACGAGACCAA GCTGGAACTG AAAGAAATCA GTGACAAGAT GCTTCTTCTA 
AATCAAGAAA AGCTCAGTCA TCAGGGACGA TTGAAAATGG CAGCAGGTGG CTCCAACAAT 
ACATATTCTC TAGCAATGAA TAACTATGGT AGCAAAGAAA TTTCACTGAA TATGCTGCGC 
ACGACTTTGT CAAACATAGA AAAGGAAACT GAGACGAAGC AACAAGATGT GAACAACAAC 
AATGCCCAAC TCAAGGAGTT GAAAGAAAAA CTGTCCGAGC TGGTGCAACA GAACGAGCTC 
CTGTTGCAAG AGTTCACGCA GAAGAAGCAG CAAGTCATGG CCTTGAAGAA GCAAAAAGGA 
AATGTTGCGT CGGTCGACGC GTGGAACGAT CAGTCGGAAT TTGCACCTGT GGACTCCTGG 
CCAGGAGATG GTGTGCAACA GACCACTACA TCTACCGGTT TGGTCAAGTA CAGAGCTCTC 
TACGCCTTTG AAGCACGCAA TGAGGATGAA CTGTCAATAA TGCCTGGGGA CATAGTCATG 
GTTCAGGAGA ACCAGCATGG GGAACCGGGG TGGCTCGGGG GAGAGCTGCG GGGCAAGACA 
GGCTGGTTTC CAGAGTCCTA CGTGGAAAAG CTGGTCTCCA CCCCGCCGGG GGCCTTTGCG 
GAGGTCCCGG GCGAGGCCGT GGCTACCGCC GCAGACGTGC TCACCGCGGC CGAGACCGTC 
CACATCGTGG GAACAGAGAT GAAGAGGACC CTAGAAGGAA TTTCCGAGGC TCCAGAAAAC 
GGCCGGGAGC ACGCCGTCGA GCTCGCCGCC GCCCCGGATG TTCTGCAGCC CTCGGAGAAC 
TCCTGCTTTA CCCAGCTGCC CAGGGTGCCC CCCATGGACA CCACTTCGCC CGTGCCCGGA 
GAGGGCCAGG CGGCTCCCCA GGGTTTGCAG GCCCAGGCCC TGTTCCCGTG GAGGGCGAAA 
AAGGAGAACC ACCTTTCCTT CAACAAAGGG GACGTAATCA CCGTCAAAGA ACAACAGGAC 
ATGTGGTGGT ACGGCGACTT CGAAGGGAAG GTTGGGTGGT TCCCAAAGTC TTACGTGAGG 
CTTGTGTCTG GGCCTATGAA GTCCTTCAAC AACAATCAAA TATCCGATTC TCAGGAGTTT 
GGAGATGTAC CTCCGGAACC TCCCAGTGAC TTCCCAGTGG TGAGCGAGCG GTACGTGGCC 
GTCTACCCGT ACCAGTCCCA GGAACCAGGC GACCTCAGCT TCCTCGCCGG GGACGTGCTC 
ATTGTGAACA AGAAGGAAGG GGAGTGGTGG ACGGGGACCA TCGGGGGCAT GACAGGCATC 
TTCCCCAGCA ACTACGTCCG CCCGTGCGAG CCCGAGGCCA ACAACAAGGG CAAAAAGAAC 
TCCATCTCTG AGGATTTCAT TCCATCTGCC ACCACATTGC CAACTAACGA AGAAGTGGGC 
CTCTCGGGAC AAGAGCCATC GAGGCCAGAC ACTCCACAAA GTGATGCAAA AGCAAAGAAC 
ATGAAGCTGA CCAAGAAGCC GGAGATAGCG TCGGTGATTG CCCCTTACAA GGCGACCGGG 
CCGGAACAAC TTACCCTGGA GAAAGGGCAG CTCATCCAAG TGCGCAAAAA AACGGAAGGT 
GGCTGGTGGG AAGGAGAACT ACAGGTCAAA GGAAAGAAGA GGCAGTCGGG ATGGTTCCCG 
GCATCTTACG TGAAAGTCCT CGGAGGTAGC GGTGCAAGCA GCAGCAGAAG TTCGCCTGTG 
CCCACTGCAT TCGCTTCCAG TACTCAAGGG GAGCAAGTGC GTGCCCTGTT CCCATTTGTG 
GCCCAACACG AGGACGAGCT GTCATTCCAG AAGGGTCAGG TGGTGACGGT GCTGAGCAAG 
GAGGACCCCT CTTGGTGGAA AGGGGAGCTG GGCGGACACG TGGGACTCTT CCCTTCCAAC 
TACGTCGAGC CACTGGATCG GGCTGACAAG TGTTCCGGGC TCCAGGACAT TCTGTTCAGC 
TCCTCCCTGA GCACGCGCGA GAAGAAGAGA CAAGGCCACA TCTACGAGCT CCTGTCCACG 
GAAGAGAACT ACCTGAAGGA CCTGACCCTA GTCAAGGAGG TTTTTTACAG GCCAATGAAA 
CAGTCCACTC TTCTAACGGA CGATGAGGTT AAACTGATCT TCGTAAATTG GCCAGAGCTC 
ATCATGTGCA ACACCAAGAT GCTCAAATCG TTTCGAGTAC GACAGCGAAT GAGCGAGAAC 
GGCATCATAG AGATGATTGG AGACATCCTC TGTGAAAATC TACCGTACCT TAACCCGTAC 
GTGAGGTTCT GCAGCTGCCA GGTCCGATCC ATGGCCTTCA TTCAGCAGAA ATCCGAAAGC 
GACCCCCGCT TCAAATCCTT CACCAGGGAT TTCTGCAAGA ACCCGAAGAC CCGCGGCATG 
CCTTTCACCA GCTTCCTTCT GAAGCCAATG CAGAGGATGA CCAAGTACCC CCTGATGATT 
AAAAAGATTC GCGAGTACAC GGACCCGACG CACCACGACC GCGGCTACCT GGACGAGGCC 
CTGGAGAAAG CGGAACAGCT CTGCAACCAG ATCAACGAAG CGGTCAAGGA GCGGGAGAAC 
TCGGACCACC TGGAGTGGGC GCAGACGCAT ATCCAGTGCA ACGGCATCTC GGAGAAACTC 
GTGTTCAATT CAACCACCAA CGTCCTCGGC TCCCGAAAGT TTTTGCACTC GGGTTCTCTC 
ACGAAAGTCA AAAGCGGGAA GGAGTTGGTG GGATTCCTTT TCAACGACTT TCTGCTGCTC 
TCGCAACCCG TTCGAGACCT GGGGAGAGTG ACCAACGTAT TCATGTCGGA TCGAGCCATG 
AACACTGCCT ACAGGTTATA CAAGCAGCCT CTATTCCTGA ACACGGTGAT GGTGCAGGAC 
ACCTCGGACG GAAGTCCCAC GGACGACACG TTCCAGATCT CGGCTCCCGA TTACATCTGC 
GTGTTCCGAG CGGCCAACCC AAACGAAAAG TTGCTTTGGG TGAAGAAGAT TAAAGACGCC 
GCGCACAACT ACCGGAACAC GGAAGCCAAG CAGATGAAGG TTTCGGGGCA GATCGATTCG 
TACTGCGAGG TGAACATGGC CTCCCAGGAG CACAAGACGC ACGTGGTGTC GAGCACCACC 
AGCCCCCAGT GGAACACGTC CATGCAGTTC CTGGTCAGGG ACCTCCGGCA AGACGTCCTC 
TGCGTCACCG TCTTCAACAA GGAGCTCTTT TCGCCCAACG ACTTCTTGGG CCGCACCGAG 
GTCCGGGTGT GGGACATCGC GCAGGAGACG CGAGAGAGCC ACGGGCCAAT CACCAAGCAG 
CTCCGGCTCT ACGAGGTGGA CCAAGGAGAA GTGCTCATCA GGCTGGACCT GCAGATCTTC 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC8032044 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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