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Phactr4 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Phactr4
Entrez Gene ID 100169
Full Name phosphatase and actin regulator 4
Synonyms 3110001B12Rik,AI527228,AW495572,C330013F19Rik,N28169,mKIAA4120
General protein information
Preferred Names

phosphatase and actin regulator 4

Names

phosphatase and actin regulator 4
humdy
protein Humpty dumpty
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

4

4|4 D2.3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006538456.3 XP_006538519.1 phosphatase and actin regulator 4 isoform X1
NM_001161797.1 NP_001155269.1 phosphatase and actin regulator 4 isoform 2
XM_006538457.3 XP_006538520.1 phosphatase and actin regulator 4 isoform X2
XM_011250172.1 XP_011248474.1 phosphatase and actin regulator 4 isoform X3
XM_006538457.4 XP_006538520.1 phosphatase and actin regulator 4 isoform X2
NM_175306.4 NP_780515.2 phosphatase and actin regulator 4 isoform 1
XM_006538456.4 XP_006538519.1 phosphatase and actin regulator 4 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) PHACTR4 NP_076412.3
Mus musculus (house mouse) Phactr4 NP_780515.2
LOC716990 XP_001113123.2
Pan troglodytes (chimpanzee) PHACTR4 XP_513255.3
Rattus norvegicus (Norway rat) Phactr4 XP_006239173.1
Canis lupus familiaris (dog) PHACTR4 XP_005617759.1
Gallus gallus (chicken) PHACTR4 XP_422879.4
Bos taurus (cattle) PHACTR4 NP_001095477.1

Gene Ontology


Bibliography

Related articles in PubMed

ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.
Rohacek AM, Bebee TW, Tilton RK, Radens CM, McDermott-Roe C, Peart N, Kaur M, Zaykaner M, Cieply B, Musunuru K, Barash Y, Germiller JA, Krantz ID, Carstens RP, Epstein DJ
Developmental cell43(3)318-331.e5(2017 11)

Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes.
Cho HM, Kim JY, Kim H, Sun W
Histochemistry and cell biology142(4)411-9(2014 Oct)

Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool.
Huet G, Rajakyl? EK, Viita T, Skarp KP, Crivaro M, Dopie J, Vartiainen MK
Journal of cell science126(Pt 2)497-507(2013 Jan)

Different expression patterns of Phactr family members in normal and injured mouse brain.
Kim JY, Choi SY, Moon Y, Kim HJ, Chin JH, Kim H, Sun W
Neuroscience22137-46(2012 Sep)

Phactr4 regulates directional migration of enteric neural crest through PP1, integrin signaling, and cofilin activity.
Zhang Y, Kim TH, Niswander L
Genes & development26(1)69-81(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Phactr4 forms functional associations with intermediate filament networks in adult neural stem cells.
Title: Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes.

Data discloses the central role of Phactr4 in a feedback loop, where actin monomers regulate their own number via the activation of a key regulator of actin dynamics.
Title: Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool.

This study demonistrated that the mRNA expression of phactr4 was progressively increased in the injury penumbra.
Title: Different expression patterns of Phactr family members in normal and injured mouse brain.

hactr4 negatively regulates integrin signaling through the RHO/ROCK pathway and coordinates protein phosphatase 1 (PP1) with cofilin activity to regulate cytoskeletal dynamics
Title: Phactr4 regulates directional migration of enteric neural crest through PP1, integrin signaling, and cofilin activity.

Phactr4 is critical for the spatially and temporally regulated transition in proliferation through differential regulation of PP1 and the cell cycle during neurulation and eye development
Title: Phactr4 regulates neural tube and optic fissure closure by controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression.

The following Phactr4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phactr4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu71995 XM_011250172.1
Latest version!		 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OMu13147 NM_001161797.1
Latest version!		 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu13228 NM_175306.4
Latest version!		 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $405.30
$579.00
OMu33928 XM_006538456.4
Latest version!		 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OMu33929 XM_006538457.4
Latest version!		 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OMu33929 XM_006538457.3 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OMu33928 XM_006538456.3 Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu71995
Clone ID Related Accession (Same CDS sequence) XM_011250172.1
Accession Version XM_011250172.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1263bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
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361
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481
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661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAACAGTG GCACAGTGCT GTCGAAACCA TCCCCACCCT TGCCACCCAA GAGAGGCATT 
CCATCAACTT CGATACCCAG CTTGGAGCCT GCTGCTTCGT TCACAACTAA GACAGCAAAT 
GATCAAAGAG AAAAAACTGT CTCTTTGTGT TTGGAACCAC CACTGATAAT CCCGCCTTCG 
TCCCCATCAC CCCCACTGCC TACCCACATA CCTCCCGAGC CTCCACGGTC CCCTCTAGTG 
CCTGCTAAGA CTTTTCAAAT TGTGCCAGAG GTTGAGTTTT CCTCTTCTTC AGATCTATTC 
CAGGACATTT CCCAGCAGGA GGATCAGAAA ACAGAAGTCC CCAAGAAGAT ACAGGATCAG 
AGCTTTGGGG AGTCCCATAT ACCCTCCAGG CTGCCCCCAC TCCCACTGCA TATCCGAATT 
CAGCAGGCCC TTACCAGCCC CCTTCCTGTG ACCCCTCCCT TGGAAGGCAC TCACAGAGCC 
CATTCACTGC TTTTTGAGAA CAGTGACAGC TTCTCCGAGG ACACTGGTAC CCTGGGTCGT 
ACCAGGTCAC TTCCCATCAC GATTGAGATG TTGAAAGTTC CAGATGATGA AGAGGAAGAG 
CAGACCTGCC CGTTTGTTGA AGACGTGACG TCTACCTCAG CCACTCCTAG CCTACCACTG 
TGTCTGCGAG AGGAAGAGAA GGAGAGTGAC TCAGATTCAG AGGGTCCCAT TAAGTACCGA 
GATGAAGAAG AAGATGATGA TGATGATGAA AGCCATCAGA GTGCCCTGGC CAACAGAGTG 
AAGAGGAAGG ACACGCTGGC AATGAAGCTG AGCAGCAGAC CCAGTGAACC AGAGACCAAC 
CTGAATTCTT GGCCTCGTAA AAGCAAGGAG GAGTGGAATG AAATACGGCA CCAGATCGGG 
AACACGCTGA TCCGGCGGCT GAGTCAGAGA CCAACAGCAG AAGAATTGGA GCAGCGGAAC 
ATACTACAGC CTAAAAATGA AGCTGATCGG CAAGCAGAGA AACGGGAAAT CAAGCGTCGC 
CTCACTAGAA AGCTCAGTCA AAGGCCAACT GTGGCTGAAC TGCTTGCCAG GAAGATTCTG 
CGGTTTAATG AGTATGTGGA AGTAACTGAT GCTCATGACT ATGACCGGCG AGCAGACAAA 
CCATGGACCA AACTGACTCC TGCTGACAAG GCTGCCATAA GGAAAGAATT AAATGAATTT 
AAGAGCTCGG AGATGGAGGT TCATGTAGAC AGCAAACACT TTACACGCTA CCATCGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248474.1
CDS742..2004
Translation

Target ORF information:

RefSeq Version XM_011250172.1
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250172.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGAACAGTG GCACAGTGCT GTCGAAACCA TCCCCACCCT TGCCACCCAA GAGAGGCATT 
CCATCAACTT CGATACCCAG CTTGGAGCCT GCTGCTTCGT TCACAACTAA GACAGCAAAT 
GATCAAAGAG AAAAAACTGT CTCTTTGTGT TTGGAACCAC CACTGATAAT CCCGCCTTCG 
TCCCCATCAC CCCCACTGCC TACCCACATA CCTCCCGAGC CTCCACGGTC CCCTCTAGTG 
CCTGCTAAGA CTTTTCAAAT TGTGCCAGAG GTTGAGTTTT CCTCTTCTTC AGATCTATTC 
CAGGACATTT CCCAGCAGGA GGATCAGAAA ACAGAAGTCC CCAAGAAGAT ACAGGATCAG 
AGCTTTGGGG AGTCCCATAT ACCCTCCAGG CTGCCCCCAC TCCCACTGCA TATCCGAATT 
CAGCAGGCCC TTACCAGCCC CCTTCCTGTG ACCCCTCCCT TGGAAGGCAC TCACAGAGCC 
CATTCACTGC TTTTTGAGAA CAGTGACAGC TTCTCCGAGG ACACTGGTAC CCTGGGTCGT 
ACCAGGTCAC TTCCCATCAC GATTGAGATG TTGAAAGTTC CAGATGATGA AGAGGAAGAG 
CAGACCTGCC CGTTTGTTGA AGACGTGACG TCTACCTCAG CCACTCCTAG CCTACCACTG 
TGTCTGCGAG AGGAAGAGAA GGAGAGTGAC TCAGATTCAG AGGGTCCCAT TAAGTACCGA 
GATGAAGAAG AAGATGATGA TGATGATGAA AGCCATCAGA GTGCCCTGGC CAACAGAGTG 
AAGAGGAAGG ACACGCTGGC AATGAAGCTG AGCAGCAGAC CCAGTGAACC AGAGACCAAC 
CTGAATTCTT GGCCTCGTAA AAGCAAGGAG GAGTGGAATG AAATACGGCA CCAGATCGGG 
AACACGCTGA TCCGGCGGCT GAGTCAGAGA CCAACAGCAG AAGAATTGGA GCAGCGGAAC 
ATACTACAGC CTAAAAATGA AGCTGATCGG CAAGCAGAGA AACGGGAAAT CAAGCGTCGC 
CTCACTAGAA AGCTCAGTCA AAGGCCAACT GTGGCTGAAC TGCTTGCCAG GAAGATTCTG 
CGGTTTAATG AGTATGTGGA AGTAACTGAT GCTCATGACT ATGACCGGCG AGCAGACAAA 
CCATGGACCA AACTGACTCC TGCTGACAAG GCTGCCATAA GGAAAGAATT AAATGAATTT 
AAGAGCTCGG AGATGGAGGT TCATGTAGAC AGCAAACACT TTACACGCTA CCATCGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu13147
Clone ID Related Accession (Same CDS sequence) NM_001161797.1
Accession Version NM_001161797.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2004bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-20
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY294063.1, BB849287.1, CN460852.1, BC075672.1 and AK029356.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter isoform (2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK220496.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAAGACC CATCGGAAGA AGCAGAGCAG CCCTCTGGAG ACCCAGGCAT GGGCATGGAC 
AGTGTGGAAG CAGGGGACAC AACACCTCCC ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA 
GGCAAGATCT TCAAGCCGTG GAAATGGAGG AAAAAAAAAA GCAGTGATAA GTTCAAAGAG 
ACATCAGAAG ATGGTGAGGA TTCAGGAAAG CTGAGCCATG CTGCATTAAA GAACGGACAC 
ACCACCCCCA TAGGCAGTGC CAGGTCCTCT AGCCCAGTCC TGGTAGAAGA AGAGCCAGAG 
AGAAGTCTCA GGAACCTCAC TCCAGAAGAG GAGTCAAAGA AGAGACTTGG CTCAACTGGA 
AGCCAGCCTA ACTCTGAAGC CGAGCCTGGT CCTGAGCATG CACCTAAGCA GCCTCTGCTG 
CCCCCCAAGA GACCCTTGTC CTCTTCTTGT GAAGCAAAGG AGGTTCCGGC TGGCAGTACA 
GCAAGGTCCG TCTCCTCCAC CTCTGGCTCT ACCACCGTGA CATCTGCTGC CACCACTGCT 
GCCACAGACA TGACAAAGAC TGTTAAGTCC TTTGTGGGCC CCACCCCCGC TCCTGCCCCC 
GCCCCCAGGA CTCTGCCTGC TGCTCCTGCC AGTGCTAACA CTGCTGCCAC CACTACAGCC 
CCTGCCAAGC AACCCCCCAT CCCACCCCCT AAACCAGCTC AGAGAAACAG CAACCCCATC 
ATTGCTGAAC TATCCCAAGC AATGAACAGT GGCACAGTGC TGTCGAAACC ATCCCCACCC 
TTGCCACCCA AGAGAGGCAT TCCATCAACT TCGATACCCA GCTTGGAGCC TGCTGCTTCG 
TTCACAACTA AGACAGCAAA TGATCAAAGA GAAAAAACTG TCTCTTTGTG TTTGGAACCA 
CCACTGATAA TCCCGCCTTC GTCCCCATCA CCCCCACTGC CTACCCACAT ACCTCCCGAG 
CCTCCACGGT CCCCTCTAGT GCCTGCTAAG ACTTTTCAAA TTGTGCCAGA GGTTGAGTTT 
TCCTCTTCTT CAGATCTATT CCAGGACATT TCCCAGCAGG AGGATCAGAA AACAGAAGTC 
CCCAAGAAGA TACAGGATCA GAGCTTTGGG GAGTCCCATA TACCCTCCAG GCTGCCCCCA 
CTCCCACTGC ATATCCGAAT TCAGCAGGCC CTTACCAGCC CCCTTCCTGT GACCCCTCCC 
TTGGAAGGCA CTCACAGAGC CCATTCACTG CTTTTTGAGA ACAGTGACAG CTTCTCCGAG 
GACACTGGTA CCCTGGGTCG TACCAGGTCA CTTCCCATCA CGATTGAGAT GTTGAAAGTT 
CCAGATGATG AAGAGGAAGA GCAGACCTGC CCGTTTGTTG AAGACGTGAC GTCTACCTCA 
GCCACTCCTA GCCTACCACT GTGTCTGCGA GAGGAAGAGA AGGAGAGTGA CTCAGATTCA 
GAGGGTCCCA TTAAGTACCG AGATGAAGAA GAAGATGATG ATGATGATGA AAGCCATCAG 
AGTGCCCTGG CCAACAGAGT GAAGAGGAAG GACACGCTGG CAATGAAGCT GAGCAGCAGA 
CCCAGTGAAC CAGAGACCAA CCTGAATTCT TGGCCTCGTA AAAGCAAGGA GGAGTGGAAT 
GAAATACGGC ACCAGATCGG GAACACGCTG ATCCGGCGGC TGAGTCAGAG ACCAACAGCA 
GAAGAATTGG AGCAGCGGAA CATACTACAG CCTAAAAATG AAGCTGATCG GCAAGCAGAG 
AAACGGGAAA TCAAGCGTCG CCTCACTAGA AAGCTCAGTC AAAGGCCAAC TGTGGCTGAA 
CTGCTTGCCA GGAAGATTCT GCGGTTTAAT GAGTATGTGG AAGTAACTGA TGCTCATGAC 
TATGACCGGC GAGCAGACAA ACCATGGACC AAACTGACTC CTGCTGACAA GGCTGCCATA 
AGGAAAGAAT TAAATGAATT TAAGAGCTCG GAGATGGAGG TTCATGTAGA CAGCAAACAC 
TTTACACGCT ACCATCGCCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001155269.1
CDS141..2144
Translation

Target ORF information:

RefSeq Version NM_001161797.1
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001161797.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGAAGACC CATCGGAAGA AGCAGAGCAG CCCTCTGGAG ACCCAGGCAT GGGCATGGAC 
AGTGTGGAAG CAGGGGACAC AACACCTCCC ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA 
GGCAAGATCT TCAAGCCGTG GAAATGGAGG AAAAAAAAAA GCAGTGATAA GTTCAAAGAG 
ACATCAGAAG ATGGTGAGGA TTCAGGAAAG CTGAGCCATG CTGCATTAAA GAACGGACAC 
ACCACCCCCA TAGGCAGTGC CAGGTCCTCT AGCCCAGTCC TGGTAGAAGA AGAGCCAGAG 
AGAAGTCTCA GGAACCTCAC TCCAGAAGAG GAGTCAAAGA AGAGACTTGG CTCAACTGGA 
AGCCAGCCTA ACTCTGAAGC CGAGCCTGGT CCTGAGCATG CACCTAAGCA GCCTCTGCTG 
CCCCCCAAGA GACCCTTGTC CTCTTCTTGT GAAGCAAAGG AGGTTCCGGC TGGCAGTACA 
GCAAGGTCCG TCTCCTCCAC CTCTGGCTCT ACCACCGTGA CATCTGCTGC CACCACTGCT 
GCCACAGACA TGACAAAGAC TGTTAAGTCC TTTGTGGGCC CCACCCCCGC TCCTGCCCCC 
GCCCCCAGGA CTCTGCCTGC TGCTCCTGCC AGTGCTAACA CTGCTGCCAC CACTACAGCC 
CCTGCCAAGC AACCCCCCAT CCCACCCCCT AAACCAGCTC AGAGAAACAG CAACCCCATC 
ATTGCTGAAC TATCCCAAGC AATGAACAGT GGCACAGTGC TGTCGAAACC ATCCCCACCC 
TTGCCACCCA AGAGAGGCAT TCCATCAACT TCGATACCCA GCTTGGAGCC TGCTGCTTCG 
TTCACAACTA AGACAGCAAA TGATCAAAGA GAAAAAACTG TCTCTTTGTG TTTGGAACCA 
CCACTGATAA TCCCGCCTTC GTCCCCATCA CCCCCACTGC CTACCCACAT ACCTCCCGAG 
CCTCCACGGT CCCCTCTAGT GCCTGCTAAG ACTTTTCAAA TTGTGCCAGA GGTTGAGTTT 
TCCTCTTCTT CAGATCTATT CCAGGACATT TCCCAGCAGG AGGATCAGAA AACAGAAGTC 
CCCAAGAAGA TACAGGATCA GAGCTTTGGG GAGTCCCATA TACCCTCCAG GCTGCCCCCA 
CTCCCACTGC ATATCCGAAT TCAGCAGGCC CTTACCAGCC CCCTTCCTGT GACCCCTCCC 
TTGGAAGGCA CTCACAGAGC CCATTCACTG CTTTTTGAGA ACAGTGACAG CTTCTCCGAG 
GACACTGGTA CCCTGGGTCG TACCAGGTCA CTTCCCATCA CGATTGAGAT GTTGAAAGTT 
CCAGATGATG AAGAGGAAGA GCAGACCTGC CCGTTTGTTG AAGACGTGAC GTCTACCTCA 
GCCACTCCTA GCCTACCACT GTGTCTGCGA GAGGAAGAGA AGGAGAGTGA CTCAGATTCA 
GAGGGTCCCA TTAAGTACCG AGATGAAGAA GAAGATGATG ATGATGATGA AAGCCATCAG 
AGTGCCCTGG CCAACAGAGT GAAGAGGAAG GACACGCTGG CAATGAAGCT GAGCAGCAGA 
CCCAGTGAAC CAGAGACCAA CCTGAATTCT TGGCCTCGTA AAAGCAAGGA GGAGTGGAAT 
GAAATACGGC ACCAGATCGG GAACACGCTG ATCCGGCGGC TGAGTCAGAG ACCAACAGCA 
GAAGAATTGG AGCAGCGGAA CATACTACAG CCTAAAAATG AAGCTGATCG GCAAGCAGAG 
AAACGGGAAA TCAAGCGTCG CCTCACTAGA AAGCTCAGTC AAAGGCCAAC TGTGGCTGAA 
CTGCTTGCCA GGAAGATTCT GCGGTTTAAT GAGTATGTGG AAGTAACTGA TGCTCATGAC 
TATGACCGGC GAGCAGACAA ACCATGGACC AAACTGACTC CTGCTGACAA GGCTGCCATA 
AGGAAAGAAT TAAATGAATT TAAGAGCTCG GAGATGGAGG TTCATGTAGA CAGCAAACAC 
TTTACACGCT ACCATCGCCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu13228
Clone ID Related Accession (Same CDS sequence) NM_175306.4
Accession Version NM_175306.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2085bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-21
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY294063.1, BB849287.1, BC075672.1 and AK029356.1. On Jun 17, 2009 this sequence version replaced NM_175306.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075672.1, AK049209.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGAAGACC CATCGGAAGA AGCAGAGCAG CCCTCTGGAG ACCCAGGCAT GGGCATGGAC 
AGTGTGGAAG CAGGGGACAC AACACCTCCC ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA 
GGCAAGATCT TCAAGCCGTG GAAATGGAGG AAAAAAAAAA GCAGTGATAA GTTCAAAGAG 
ACATCAGAAG TTTTAGAGCG AAAAATATCT ATGCGAAAAC CAAGAGAAGA ACTGGTTAAA 
CGAGGGGTTC TGTTGGAGGA CCCCGAGCAG GATGGTGAGG ATTCAGGAAA GCTGAGCCAT 
GCTGCATTAA AGAACGGACA CACCACCCCC ATAGGCAGTG CCAGGTCCTC TAGCCCAGTC 
CTGGTAGAAG AAGAGCCAGA GAGAAGTCTC AGGAACCTCA CTCCAGAAGA GGAGTCAAAG 
AAGAGACTTG GCTCAACTGG AAGCCAGCCT AACTCTGAAG CCGAGCCTGG TCCTGAGCAT 
GCACCTAAGC AGCCTCTGCT GCCCCCCAAG AGACCCTTGT CCTCTTCTTG TGAAGCAAAG 
GAGGTTCCGG CTGGCAGTAC AGCAAGGTCC GTCTCCTCCA CCTCTGGCTC TACCACCGTG 
ACATCTGCTG CCACCACTGC TGCCACAGAC ATGACAAAGA CTGTTAAGTC CTTTGTGGGC 
CCCACCCCCG CTCCTGCCCC CGCCCCCAGG ACTCTGCCTG CTGCTCCTGC CAGTGCTAAC 
ACTGCTGCCA CCACTACAGC CCCTGCCAAG CAACCCCCCA TCCCACCCCC TAAACCAGCT 
CAGAGAAACA GCAACCCCAT CATTGCTGAA CTATCCCAAG CAATGAACAG TGGCACAGTG 
CTGTCGAAAC CATCCCCACC CTTGCCACCC AAGAGAGGCA TTCCATCAAC TTCGATACCC 
AGCTTGGAGC CTGCTGCTTC GTTCACAACT AAGACAGCAA ATGATCAAAG AGAAAAAACT 
GTCTCTTTGT GTTTGGAACC ACCACTGATA ATCCCGCCTT CGTCCCCATC ACCCCCACTG 
CCTACCCACA TACCTCCCGA GCCTCCACGG TCCCCTCTAG TGCCTGCTAA GACTTTTCAA 
ATTGTGCCAG AGGTTGAGTT TTCCTCTTCT TCAGATCTAT TCCAGGACAT TTCCCAGCAG 
GAGGATCAGA AAACAGAAGT CCCCAAGAAG ATACAGGATC AGAGCTTTGG GGAGTCCCAT 
ATACCCTCCA GGCTGCCCCC ACTCCCACTG CATATCCGAA TTCAGCAGGC CCTTACCAGC 
CCCCTTCCTG TGACCCCTCC CTTGGAAGGC ACTCACAGAG CCCATTCACT GCTTTTTGAG 
AACAGTGACA GCTTCTCCGA GGACACTGGT ACCCTGGGTC GTACCAGGTC ACTTCCCATC 
ACGATTGAGA TGTTGAAAGT TCCAGATGAT GAAGAGGAAG AGCAGACCTG CCCGTTTGTT 
GAAGACGTGA CGTCTACCTC AGCCACTCCT AGCCTACCAC TGTGTCTGCG AGAGGAAGAG 
AAGGAGAGTG ACTCAGATTC AGAGGGTCCC ATTAAGTACC GAGATGAAGA AGAAGATGAT 
GATGATGATG AAAGCCATCA GAGTGCCCTG GCCAACAGAG TGAAGAGGAA GGACACGCTG 
GCAATGAAGC TGAGCAGCAG ACCCAGTGAA CCAGAGACCA ACCTGAATTC TTGGCCTCGT 
AAAAGCAAGG AGGAGTGGAA TGAAATACGG CACCAGATCG GGAACACGCT GATCCGGCGG 
CTGAGTCAGA GACCAACAGC AGAAGAATTG GAGCAGCGGA ACATACTACA GCCTAAAAAT 
GAAGCTGATC GGCAAGCAGA GAAACGGGAA ATCAAGCGTC GCCTCACTAG AAAGCTCAGT 
CAAAGGCCAA CTGTGGCTGA ACTGCTTGCC AGGAAGATTC TGCGGTTTAA TGAGTATGTG 
GAAGTAACTG ATGCTCATGA CTATGACCGG CGAGCAGACA AACCATGGAC CAAACTGACT 
CCTGCTGACA AGGCTGCCAT AAGGAAAGAA TTAAATGAAT TTAAGAGCTC GGAGATGGAG 
GTTCATGTAG ACAGCAAACA CTTTACACGC TACCATCGCC CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_780515.2
CDS141..2225
Translation

Target ORF information:

RefSeq Version NM_175306.4
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175306.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGAAGACC CATCGGAAGA AGCAGAGCAG CCCTCTGGAG ACCCAGGCAT GGGCATGGAC 
AGTGTGGAAG CAGGGGACAC AACACCTCCC ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA 
GGCAAGATCT TCAAGCCGTG GAAATGGAGG AAAAAAAAAA GCAGTGATAA GTTCAAAGAG 
ACATCAGAAG TTTTAGAGCG AAAAATATCT ATGCGAAAAC CAAGAGAAGA ACTGGTTAAA 
CGAGGGGTTC TGTTGGAGGA CCCCGAGCAG GATGGTGAGG ATTCAGGAAA GCTGAGCCAT 
GCTGCATTAA AGAACGGACA CACCACCCCC ATAGGCAGTG CCAGGTCCTC TAGCCCAGTC 
CTGGTAGAAG AAGAGCCAGA GAGAAGTCTC AGGAACCTCA CTCCAGAAGA GGAGTCAAAG 
AAGAGACTTG GCTCAACTGG AAGCCAGCCT AACTCTGAAG CCGAGCCTGG TCCTGAGCAT 
GCACCTAAGC AGCCTCTGCT GCCCCCCAAG AGACCCTTGT CCTCTTCTTG TGAAGCAAAG 
GAGGTTCCGG CTGGCAGTAC AGCAAGGTCC GTCTCCTCCA CCTCTGGCTC TACCACCGTG 
ACATCTGCTG CCACCACTGC TGCCACAGAC ATGACAAAGA CTGTTAAGTC CTTTGTGGGC 
CCCACCCCCG CTCCTGCCCC CGCCCCCAGG ACTCTGCCTG CTGCTCCTGC CAGTGCTAAC 
ACTGCTGCCA CCACTACAGC CCCTGCCAAG CAACCCCCCA TCCCACCCCC TAAACCAGCT 
CAGAGAAACA GCAACCCCAT CATTGCTGAA CTATCCCAAG CAATGAACAG TGGCACAGTG 
CTGTCGAAAC CATCCCCACC CTTGCCACCC AAGAGAGGCA TTCCATCAAC TTCGATACCC 
AGCTTGGAGC CTGCTGCTTC GTTCACAACT AAGACAGCAA ATGATCAAAG AGAAAAAACT 
GTCTCTTTGT GTTTGGAACC ACCACTGATA ATCCCGCCTT CGTCCCCATC ACCCCCACTG 
CCTACCCACA TACCTCCCGA GCCTCCACGG TCCCCTCTAG TGCCTGCTAA GACTTTTCAA 
ATTGTGCCAG AGGTTGAGTT TTCCTCTTCT TCAGATCTAT TCCAGGACAT TTCCCAGCAG 
GAGGATCAGA AAACAGAAGT CCCCAAGAAG ATACAGGATC AGAGCTTTGG GGAGTCCCAT 
ATACCCTCCA GGCTGCCCCC ACTCCCACTG CATATCCGAA TTCAGCAGGC CCTTACCAGC 
CCCCTTCCTG TGACCCCTCC CTTGGAAGGC ACTCACAGAG CCCATTCACT GCTTTTTGAG 
AACAGTGACA GCTTCTCCGA GGACACTGGT ACCCTGGGTC GTACCAGGTC ACTTCCCATC 
ACGATTGAGA TGTTGAAAGT TCCAGATGAT GAAGAGGAAG AGCAGACCTG CCCGTTTGTT 
GAAGACGTGA CGTCTACCTC AGCCACTCCT AGCCTACCAC TGTGTCTGCG AGAGGAAGAG 
AAGGAGAGTG ACTCAGATTC AGAGGGTCCC ATTAAGTACC GAGATGAAGA AGAAGATGAT 
GATGATGATG AAAGCCATCA GAGTGCCCTG GCCAACAGAG TGAAGAGGAA GGACACGCTG 
GCAATGAAGC TGAGCAGCAG ACCCAGTGAA CCAGAGACCA ACCTGAATTC TTGGCCTCGT 
AAAAGCAAGG AGGAGTGGAA TGAAATACGG CACCAGATCG GGAACACGCT GATCCGGCGG 
CTGAGTCAGA GACCAACAGC AGAAGAATTG GAGCAGCGGA ACATACTACA GCCTAAAAAT 
GAAGCTGATC GGCAAGCAGA GAAACGGGAA ATCAAGCGTC GCCTCACTAG AAAGCTCAGT 
CAAAGGCCAA CTGTGGCTGA ACTGCTTGCC AGGAAGATTC TGCGGTTTAA TGAGTATGTG 
GAAGTAACTG ATGCTCATGA CTATGACCGG CGAGCAGACA AACCATGGAC CAAACTGACT 
CCTGCTGACA AGGCTGCCAT AAGGAAAGAA TTAAATGAAT TTAAGAGCTC GGAGATGGAG 
GTTCATGTAG ACAGCAAACA CTTTACACGC TACCATCGCC CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33928
Clone ID Related Accession (Same CDS sequence) XM_006538456.3 , XM_006538456.4
Accession Version XM_006538456.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2115bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538456.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG TTTTAGAGCG AAAAATATCT 
ATGCGAAAAC CAAGAGAAGA ACTGGTTAAA CGAGGGGTTC TGTTGGAGGA CCCCGAGCAG 
GATGGTGAGG ATTCAGGAAA GCTGAGCCAT GCTGCATTAA AGAACGGACA CACCACCCCC 
ATAGGCAGTG CCAGGTCCTC TAGCCCAGTC CTGGTAGAAG AAGAGCCAGA GAGAAGTCTC 
AGGAACCTCA CTCCAGAAGA GGAGTCAAAG AAGAGACTTG GCTCAACTGG AAGCCAGCCT 
AACTCTGAAG CCGAGCCTGG TCCTGAGCAT GCACCTAAGC AGCCTCTGCT GCCCCCCAAG 
AGACCCTTGT CCTCTTCTTG TGAAGCAAAG GAGGTTCCGG CTGGCAGTAC AGCAAGGTCC 
GTCTCCTCCA CCTCTGGCTC TACCACCGTG ACATCTGCTG CCACCACTGC TGCCACAGAC 
ATGACAAAGA CTGTTAAGTC CTTTGTGGGC CCCACCCCCG CTCCTGCCCC CGCCCCCAGG 
ACTCTGCCTG CTGCTCCTGC CAGTGCTAAC ACTGCTGCCA CCACTACAGC CCCTGCCAAG 
CAACCCCCCA TCCCACCCCC TAAACCAGCT CAGAGAAACA GCAACCCCAT CATTGCTGAA 
CTATCCCAAG CAATGAACAG TGGCACAGTG CTGTCGAAAC CATCCCCACC CTTGCCACCC 
AAGAGAGGCA TTCCATCAAC TTCGATACCC AGCTTGGAGC CTGCTGCTTC GTTCACAACT 
AAGACAGCAA ATGATCAAAG AGAAAAAACT GTCTCTTTGT GTTTGGAACC ACCACTGATA 
ATCCCGCCTT CGTCCCCATC ACCCCCACTG CCTACCCACA TACCTCCCGA GCCTCCACGG 
TCCCCTCTAG TGCCTGCTAA GACTTTTCAA ATTGTGCCAG AGGTTGAGTT TTCCTCTTCT 
TCAGATCTAT TCCAGGACAT TTCCCAGCAG GAGGATCAGA AAACAGAAGT CCCCAAGAAG 
ATACAGGATC AGAGCTTTGG GGAGTCCCAT ATACCCTCCA GGCTGCCCCC ACTCCCACTG 
CATATCCGAA TTCAGCAGGC CCTTACCAGC CCCCTTCCTG TGACCCCTCC CTTGGAAGGC 
ACTCACAGAG CCCATTCACT GCTTTTTGAG AACAGTGACA GCTTCTCCGA GGACACTGGT 
ACCCTGGGTC GTACCAGGTC ACTTCCCATC ACGATTGAGA TGTTGAAAGT TCCAGATGAT 
GAAGAGGAAG AGCAGACCTG CCCGTTTGTT GAAGACGTGA CGTCTACCTC AGCCACTCCT 
AGCCTACCAC TGTGTCTGCG AGAGGAAGAG AAGGAGAGTG ACTCAGATTC AGAGGGTCCC 
ATTAAGTACC GAGATGAAGA AGAAGATGAT GATGATGATG AAAGCCATCA GAGTGCCCTG 
GCCAACAGAG TGAAGAGGAA GGACACGCTG GCAATGAAGC TGAGCAGCAG ACCCAGTGAA 
CCAGAGACCA ACCTGAATTC TTGGCCTCGT AAAAGCAAGG AGGAGTGGAA TGAAATACGG 
CACCAGATCG GGAACACGCT GATCCGGCGG CTGAGTCAGA GACCAACAGC AGAAGAATTG 
GAGCAGCGGA ACATACTACA GCCTAAAAAT GAAGCTGATC GGCAAGCAGA GAAACGGGAA 
ATCAAGCGTC GCCTCACTAG AAAGCTCAGT CAAAGGCCAA CTGTGGCTGA ACTGCTTGCC 
AGGAAGATTC TGCGGTTTAA TGAGTATGTG GAAGTAACTG ATGCTCATGA CTATGACCGG 
CGAGCAGACA AACCATGGAC CAAACTGACT CCTGCTGACA AGGCTGCCAT AAGGAAAGAA 
TTAAATGAAT TTAAGAGCTC GGAGATGGAG GTTCATGTAG ACAGCAAACA CTTTACACGC 
TACCATCGCC CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006538519.1
CDS2046..4160
Translation

Target ORF information:

RefSeq Version XM_006538456.3
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006538456.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG TTTTAGAGCG AAAAATATCT 
ATGCGAAAAC CAAGAGAAGA ACTGGTTAAA CGAGGGGTTC TGTTGGAGGA CCCCGAGCAG 
GATGGTGAGG ATTCAGGAAA GCTGAGCCAT GCTGCATTAA AGAACGGACA CACCACCCCC 
ATAGGCAGTG CCAGGTCCTC TAGCCCAGTC CTGGTAGAAG AAGAGCCAGA GAGAAGTCTC 
AGGAACCTCA CTCCAGAAGA GGAGTCAAAG AAGAGACTTG GCTCAACTGG AAGCCAGCCT 
AACTCTGAAG CCGAGCCTGG TCCTGAGCAT GCACCTAAGC AGCCTCTGCT GCCCCCCAAG 
AGACCCTTGT CCTCTTCTTG TGAAGCAAAG GAGGTTCCGG CTGGCAGTAC AGCAAGGTCC 
GTCTCCTCCA CCTCTGGCTC TACCACCGTG ACATCTGCTG CCACCACTGC TGCCACAGAC 
ATGACAAAGA CTGTTAAGTC CTTTGTGGGC CCCACCCCCG CTCCTGCCCC CGCCCCCAGG 
ACTCTGCCTG CTGCTCCTGC CAGTGCTAAC ACTGCTGCCA CCACTACAGC CCCTGCCAAG 
CAACCCCCCA TCCCACCCCC TAAACCAGCT CAGAGAAACA GCAACCCCAT CATTGCTGAA 
CTATCCCAAG CAATGAACAG TGGCACAGTG CTGTCGAAAC CATCCCCACC CTTGCCACCC 
AAGAGAGGCA TTCCATCAAC TTCGATACCC AGCTTGGAGC CTGCTGCTTC GTTCACAACT 
AAGACAGCAA ATGATCAAAG AGAAAAAACT GTCTCTTTGT GTTTGGAACC ACCACTGATA 
ATCCCGCCTT CGTCCCCATC ACCCCCACTG CCTACCCACA TACCTCCCGA GCCTCCACGG 
TCCCCTCTAG TGCCTGCTAA GACTTTTCAA ATTGTGCCAG AGGTTGAGTT TTCCTCTTCT 
TCAGATCTAT TCCAGGACAT TTCCCAGCAG GAGGATCAGA AAACAGAAGT CCCCAAGAAG 
ATACAGGATC AGAGCTTTGG GGAGTCCCAT ATACCCTCCA GGCTGCCCCC ACTCCCACTG 
CATATCCGAA TTCAGCAGGC CCTTACCAGC CCCCTTCCTG TGACCCCTCC CTTGGAAGGC 
ACTCACAGAG CCCATTCACT GCTTTTTGAG AACAGTGACA GCTTCTCCGA GGACACTGGT 
ACCCTGGGTC GTACCAGGTC ACTTCCCATC ACGATTGAGA TGTTGAAAGT TCCAGATGAT 
GAAGAGGAAG AGCAGACCTG CCCGTTTGTT GAAGACGTGA CGTCTACCTC AGCCACTCCT 
AGCCTACCAC TGTGTCTGCG AGAGGAAGAG AAGGAGAGTG ACTCAGATTC AGAGGGTCCC 
ATTAAGTACC GAGATGAAGA AGAAGATGAT GATGATGATG AAAGCCATCA GAGTGCCCTG 
GCCAACAGAG TGAAGAGGAA GGACACGCTG GCAATGAAGC TGAGCAGCAG ACCCAGTGAA 
CCAGAGACCA ACCTGAATTC TTGGCCTCGT AAAAGCAAGG AGGAGTGGAA TGAAATACGG 
CACCAGATCG GGAACACGCT GATCCGGCGG CTGAGTCAGA GACCAACAGC AGAAGAATTG 
GAGCAGCGGA ACATACTACA GCCTAAAAAT GAAGCTGATC GGCAAGCAGA GAAACGGGAA 
ATCAAGCGTC GCCTCACTAG AAAGCTCAGT CAAAGGCCAA CTGTGGCTGA ACTGCTTGCC 
AGGAAGATTC TGCGGTTTAA TGAGTATGTG GAAGTAACTG ATGCTCATGA CTATGACCGG 
CGAGCAGACA AACCATGGAC CAAACTGACT CCTGCTGACA AGGCTGCCAT AAGGAAAGAA 
TTAAATGAAT TTAAGAGCTC GGAGATGGAG GTTCATGTAG ACAGCAAACA CTTTACACGC 
TACCATCGCC CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33929
Clone ID Related Accession (Same CDS sequence) XM_006538457.3 , XM_006538457.4
Accession Version XM_006538457.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG ATGGTGAGGA TTCAGGAAAG 
CTGAGCCATG CTGCATTAAA GAACGGACAC ACCACCCCCA TAGGCAGTGC CAGGTCCTCT 
AGCCCAGTCC TGGTAGAAGA AGAGCCAGAG AGAAGTCTCA GGAACCTCAC TCCAGAAGAG 
GAGTCAAAGA AGAGACTTGG CTCAACTGGA AGCCAGCCTA ACTCTGAAGC CGAGCCTGGT 
CCTGAGCATG CACCTAAGCA GCCTCTGCTG CCCCCCAAGA GACCCTTGTC CTCTTCTTGT 
GAAGCAAAGG AGGTTCCGGC TGGCAGTACA GCAAGGTCCG TCTCCTCCAC CTCTGGCTCT 
ACCACCGTGA CATCTGCTGC CACCACTGCT GCCACAGACA TGACAAAGAC TGTTAAGTCC 
TTTGTGGGCC CCACCCCCGC TCCTGCCCCC GCCCCCAGGA CTCTGCCTGC TGCTCCTGCC 
AGTGCTAACA CTGCTGCCAC CACTACAGCC CCTGCCAAGC AACCCCCCAT CCCACCCCCT 
AAACCAGCTC AGAGAAACAG CAACCCCATC ATTGCTGAAC TATCCCAAGC AATGAACAGT 
GGCACAGTGC TGTCGAAACC ATCCCCACCC TTGCCACCCA AGAGAGGCAT TCCATCAACT 
TCGATACCCA GCTTGGAGCC TGCTGCTTCG TTCACAACTA AGACAGCAAA TGATCAAAGA 
GAAAAAACTG TCTCTTTGTG TTTGGAACCA CCACTGATAA TCCCGCCTTC GTCCCCATCA 
CCCCCACTGC CTACCCACAT ACCTCCCGAG CCTCCACGGT CCCCTCTAGT GCCTGCTAAG 
ACTTTTCAAA TTGTGCCAGA GGTTGAGTTT TCCTCTTCTT CAGATCTATT CCAGGACATT 
TCCCAGCAGG AGGATCAGAA AACAGAAGTC CCCAAGAAGA TACAGGATCA GAGCTTTGGG 
GAGTCCCATA TACCCTCCAG GCTGCCCCCA CTCCCACTGC ATATCCGAAT TCAGCAGGCC 
CTTACCAGCC CCCTTCCTGT GACCCCTCCC TTGGAAGGCA CTCACAGAGC CCATTCACTG 
CTTTTTGAGA ACAGTGACAG CTTCTCCGAG GACACTGGTA CCCTGGGTCG TACCAGGTCA 
CTTCCCATCA CGATTGAGAT GTTGAAAGTT CCAGATGATG AAGAGGAAGA GCAGACCTGC 
CCGTTTGTTG AAGACGTGAC GTCTACCTCA GCCACTCCTA GCCTACCACT GTGTCTGCGA 
GAGGAAGAGA AGGAGAGTGA CTCAGATTCA GAGGGTCCCA TTAAGTACCG AGATGAAGAA 
GAAGATGATG ATGATGATGA AAGCCATCAG AGTGCCCTGG CCAACAGAGT GAAGAGGAAG 
GACACGCTGG CAATGAAGCT GAGCAGCAGA CCCAGTGAAC CAGAGACCAA CCTGAATTCT 
TGGCCTCGTA AAAGCAAGGA GGAGTGGAAT GAAATACGGC ACCAGATCGG GAACACGCTG 
ATCCGGCGGC TGAGTCAGAG ACCAACAGCA GAAGAATTGG AGCAGCGGAA CATACTACAG 
CCTAAAAATG AAGCTGATCG GCAAGCAGAG AAACGGGAAA TCAAGCGTCG CCTCACTAGA 
AAGCTCAGTC AAAGGCCAAC TGTGGCTGAA CTGCTTGCCA GGAAGATTCT GCGGTTTAAT 
GAGTATGTGG AAGTAACTGA TGCTCATGAC TATGACCGGC GAGCAGACAA ACCATGGACC 
AAACTGACTC CTGCTGACAA GGCTGCCATA AGGAAAGAAT TAAATGAATT TAAGAGCTCG 
GAGATGGAGG TTCATGTAGA CAGCAAACAC TTTACACGCT ACCATCGCCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006538520.1
CDS2044..4077
Translation

Target ORF information:

RefSeq Version XM_006538457.3
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006538457.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG ATGGTGAGGA TTCAGGAAAG 
CTGAGCCATG CTGCATTAAA GAACGGACAC ACCACCCCCA TAGGCAGTGC CAGGTCCTCT 
AGCCCAGTCC TGGTAGAAGA AGAGCCAGAG AGAAGTCTCA GGAACCTCAC TCCAGAAGAG 
GAGTCAAAGA AGAGACTTGG CTCAACTGGA AGCCAGCCTA ACTCTGAAGC CGAGCCTGGT 
CCTGAGCATG CACCTAAGCA GCCTCTGCTG CCCCCCAAGA GACCCTTGTC CTCTTCTTGT 
GAAGCAAAGG AGGTTCCGGC TGGCAGTACA GCAAGGTCCG TCTCCTCCAC CTCTGGCTCT 
ACCACCGTGA CATCTGCTGC CACCACTGCT GCCACAGACA TGACAAAGAC TGTTAAGTCC 
TTTGTGGGCC CCACCCCCGC TCCTGCCCCC GCCCCCAGGA CTCTGCCTGC TGCTCCTGCC 
AGTGCTAACA CTGCTGCCAC CACTACAGCC CCTGCCAAGC AACCCCCCAT CCCACCCCCT 
AAACCAGCTC AGAGAAACAG CAACCCCATC ATTGCTGAAC TATCCCAAGC AATGAACAGT 
GGCACAGTGC TGTCGAAACC ATCCCCACCC TTGCCACCCA AGAGAGGCAT TCCATCAACT 
TCGATACCCA GCTTGGAGCC TGCTGCTTCG TTCACAACTA AGACAGCAAA TGATCAAAGA 
GAAAAAACTG TCTCTTTGTG TTTGGAACCA CCACTGATAA TCCCGCCTTC GTCCCCATCA 
CCCCCACTGC CTACCCACAT ACCTCCCGAG CCTCCACGGT CCCCTCTAGT GCCTGCTAAG 
ACTTTTCAAA TTGTGCCAGA GGTTGAGTTT TCCTCTTCTT CAGATCTATT CCAGGACATT 
TCCCAGCAGG AGGATCAGAA AACAGAAGTC CCCAAGAAGA TACAGGATCA GAGCTTTGGG 
GAGTCCCATA TACCCTCCAG GCTGCCCCCA CTCCCACTGC ATATCCGAAT TCAGCAGGCC 
CTTACCAGCC CCCTTCCTGT GACCCCTCCC TTGGAAGGCA CTCACAGAGC CCATTCACTG 
CTTTTTGAGA ACAGTGACAG CTTCTCCGAG GACACTGGTA CCCTGGGTCG TACCAGGTCA 
CTTCCCATCA CGATTGAGAT GTTGAAAGTT CCAGATGATG AAGAGGAAGA GCAGACCTGC 
CCGTTTGTTG AAGACGTGAC GTCTACCTCA GCCACTCCTA GCCTACCACT GTGTCTGCGA 
GAGGAAGAGA AGGAGAGTGA CTCAGATTCA GAGGGTCCCA TTAAGTACCG AGATGAAGAA 
GAAGATGATG ATGATGATGA AAGCCATCAG AGTGCCCTGG CCAACAGAGT GAAGAGGAAG 
GACACGCTGG CAATGAAGCT GAGCAGCAGA CCCAGTGAAC CAGAGACCAA CCTGAATTCT 
TGGCCTCGTA AAAGCAAGGA GGAGTGGAAT GAAATACGGC ACCAGATCGG GAACACGCTG 
ATCCGGCGGC TGAGTCAGAG ACCAACAGCA GAAGAATTGG AGCAGCGGAA CATACTACAG 
CCTAAAAATG AAGCTGATCG GCAAGCAGAG AAACGGGAAA TCAAGCGTCG CCTCACTAGA 
AAGCTCAGTC AAAGGCCAAC TGTGGCTGAA CTGCTTGCCA GGAAGATTCT GCGGTTTAAT 
GAGTATGTGG AAGTAACTGA TGCTCATGAC TATGACCGGC GAGCAGACAA ACCATGGACC 
AAACTGACTC CTGCTGACAA GGCTGCCATA AGGAAAGAAT TAAATGAATT TAAGAGCTCG 
GAGATGGAGG TTCATGTAGA CAGCAAACAC TTTACACGCT ACCATCGCCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33928
Clone ID Related Accession (Same CDS sequence) XM_006538456.3 , XM_006538456.4
Accession Version XM_006538456.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2115bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538456.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG TTTTAGAGCG AAAAATATCT 
ATGCGAAAAC CAAGAGAAGA ACTGGTTAAA CGAGGGGTTC TGTTGGAGGA CCCCGAGCAG 
GATGGTGAGG ATTCAGGAAA GCTGAGCCAT GCTGCATTAA AGAACGGACA CACCACCCCC 
ATAGGCAGTG CCAGGTCCTC TAGCCCAGTC CTGGTAGAAG AAGAGCCAGA GAGAAGTCTC 
AGGAACCTCA CTCCAGAAGA GGAGTCAAAG AAGAGACTTG GCTCAACTGG AAGCCAGCCT 
AACTCTGAAG CCGAGCCTGG TCCTGAGCAT GCACCTAAGC AGCCTCTGCT GCCCCCCAAG 
AGACCCTTGT CCTCTTCTTG TGAAGCAAAG GAGGTTCCGG CTGGCAGTAC AGCAAGGTCC 
GTCTCCTCCA CCTCTGGCTC TACCACCGTG ACATCTGCTG CCACCACTGC TGCCACAGAC 
ATGACAAAGA CTGTTAAGTC CTTTGTGGGC CCCACCCCCG CTCCTGCCCC CGCCCCCAGG 
ACTCTGCCTG CTGCTCCTGC CAGTGCTAAC ACTGCTGCCA CCACTACAGC CCCTGCCAAG 
CAACCCCCCA TCCCACCCCC TAAACCAGCT CAGAGAAACA GCAACCCCAT CATTGCTGAA 
CTATCCCAAG CAATGAACAG TGGCACAGTG CTGTCGAAAC CATCCCCACC CTTGCCACCC 
AAGAGAGGCA TTCCATCAAC TTCGATACCC AGCTTGGAGC CTGCTGCTTC GTTCACAACT 
AAGACAGCAA ATGATCAAAG AGAAAAAACT GTCTCTTTGT GTTTGGAACC ACCACTGATA 
ATCCCGCCTT CGTCCCCATC ACCCCCACTG CCTACCCACA TACCTCCCGA GCCTCCACGG 
TCCCCTCTAG TGCCTGCTAA GACTTTTCAA ATTGTGCCAG AGGTTGAGTT TTCCTCTTCT 
TCAGATCTAT TCCAGGACAT TTCCCAGCAG GAGGATCAGA AAACAGAAGT CCCCAAGAAG 
ATACAGGATC AGAGCTTTGG GGAGTCCCAT ATACCCTCCA GGCTGCCCCC ACTCCCACTG 
CATATCCGAA TTCAGCAGGC CCTTACCAGC CCCCTTCCTG TGACCCCTCC CTTGGAAGGC 
ACTCACAGAG CCCATTCACT GCTTTTTGAG AACAGTGACA GCTTCTCCGA GGACACTGGT 
ACCCTGGGTC GTACCAGGTC ACTTCCCATC ACGATTGAGA TGTTGAAAGT TCCAGATGAT 
GAAGAGGAAG AGCAGACCTG CCCGTTTGTT GAAGACGTGA CGTCTACCTC AGCCACTCCT 
AGCCTACCAC TGTGTCTGCG AGAGGAAGAG AAGGAGAGTG ACTCAGATTC AGAGGGTCCC 
ATTAAGTACC GAGATGAAGA AGAAGATGAT GATGATGATG AAAGCCATCA GAGTGCCCTG 
GCCAACAGAG TGAAGAGGAA GGACACGCTG GCAATGAAGC TGAGCAGCAG ACCCAGTGAA 
CCAGAGACCA ACCTGAATTC TTGGCCTCGT AAAAGCAAGG AGGAGTGGAA TGAAATACGG 
CACCAGATCG GGAACACGCT GATCCGGCGG CTGAGTCAGA GACCAACAGC AGAAGAATTG 
GAGCAGCGGA ACATACTACA GCCTAAAAAT GAAGCTGATC GGCAAGCAGA GAAACGGGAA 
ATCAAGCGTC GCCTCACTAG AAAGCTCAGT CAAAGGCCAA CTGTGGCTGA ACTGCTTGCC 
AGGAAGATTC TGCGGTTTAA TGAGTATGTG GAAGTAACTG ATGCTCATGA CTATGACCGG 
CGAGCAGACA AACCATGGAC CAAACTGACT CCTGCTGACA AGGCTGCCAT AAGGAAAGAA 
TTAAATGAAT TTAAGAGCTC GGAGATGGAG GTTCATGTAG ACAGCAAACA CTTTACACGC 
TACCATCGCC CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006538519.1
CDS2048..4162
Translation

Target ORF information:

RefSeq Version XM_006538456.4
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006538456.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG TTTTAGAGCG AAAAATATCT 
ATGCGAAAAC CAAGAGAAGA ACTGGTTAAA CGAGGGGTTC TGTTGGAGGA CCCCGAGCAG 
GATGGTGAGG ATTCAGGAAA GCTGAGCCAT GCTGCATTAA AGAACGGACA CACCACCCCC 
ATAGGCAGTG CCAGGTCCTC TAGCCCAGTC CTGGTAGAAG AAGAGCCAGA GAGAAGTCTC 
AGGAACCTCA CTCCAGAAGA GGAGTCAAAG AAGAGACTTG GCTCAACTGG AAGCCAGCCT 
AACTCTGAAG CCGAGCCTGG TCCTGAGCAT GCACCTAAGC AGCCTCTGCT GCCCCCCAAG 
AGACCCTTGT CCTCTTCTTG TGAAGCAAAG GAGGTTCCGG CTGGCAGTAC AGCAAGGTCC 
GTCTCCTCCA CCTCTGGCTC TACCACCGTG ACATCTGCTG CCACCACTGC TGCCACAGAC 
ATGACAAAGA CTGTTAAGTC CTTTGTGGGC CCCACCCCCG CTCCTGCCCC CGCCCCCAGG 
ACTCTGCCTG CTGCTCCTGC CAGTGCTAAC ACTGCTGCCA CCACTACAGC CCCTGCCAAG 
CAACCCCCCA TCCCACCCCC TAAACCAGCT CAGAGAAACA GCAACCCCAT CATTGCTGAA 
CTATCCCAAG CAATGAACAG TGGCACAGTG CTGTCGAAAC CATCCCCACC CTTGCCACCC 
AAGAGAGGCA TTCCATCAAC TTCGATACCC AGCTTGGAGC CTGCTGCTTC GTTCACAACT 
AAGACAGCAA ATGATCAAAG AGAAAAAACT GTCTCTTTGT GTTTGGAACC ACCACTGATA 
ATCCCGCCTT CGTCCCCATC ACCCCCACTG CCTACCCACA TACCTCCCGA GCCTCCACGG 
TCCCCTCTAG TGCCTGCTAA GACTTTTCAA ATTGTGCCAG AGGTTGAGTT TTCCTCTTCT 
TCAGATCTAT TCCAGGACAT TTCCCAGCAG GAGGATCAGA AAACAGAAGT CCCCAAGAAG 
ATACAGGATC AGAGCTTTGG GGAGTCCCAT ATACCCTCCA GGCTGCCCCC ACTCCCACTG 
CATATCCGAA TTCAGCAGGC CCTTACCAGC CCCCTTCCTG TGACCCCTCC CTTGGAAGGC 
ACTCACAGAG CCCATTCACT GCTTTTTGAG AACAGTGACA GCTTCTCCGA GGACACTGGT 
ACCCTGGGTC GTACCAGGTC ACTTCCCATC ACGATTGAGA TGTTGAAAGT TCCAGATGAT 
GAAGAGGAAG AGCAGACCTG CCCGTTTGTT GAAGACGTGA CGTCTACCTC AGCCACTCCT 
AGCCTACCAC TGTGTCTGCG AGAGGAAGAG AAGGAGAGTG ACTCAGATTC AGAGGGTCCC 
ATTAAGTACC GAGATGAAGA AGAAGATGAT GATGATGATG AAAGCCATCA GAGTGCCCTG 
GCCAACAGAG TGAAGAGGAA GGACACGCTG GCAATGAAGC TGAGCAGCAG ACCCAGTGAA 
CCAGAGACCA ACCTGAATTC TTGGCCTCGT AAAAGCAAGG AGGAGTGGAA TGAAATACGG 
CACCAGATCG GGAACACGCT GATCCGGCGG CTGAGTCAGA GACCAACAGC AGAAGAATTG 
GAGCAGCGGA ACATACTACA GCCTAAAAAT GAAGCTGATC GGCAAGCAGA GAAACGGGAA 
ATCAAGCGTC GCCTCACTAG AAAGCTCAGT CAAAGGCCAA CTGTGGCTGA ACTGCTTGCC 
AGGAAGATTC TGCGGTTTAA TGAGTATGTG GAAGTAACTG ATGCTCATGA CTATGACCGG 
CGAGCAGACA AACCATGGAC CAAACTGACT CCTGCTGACA AGGCTGCCAT AAGGAAAGAA 
TTAAATGAAT TTAAGAGCTC GGAGATGGAG GTTCATGTAG ACAGCAAACA CTTTACACGC 
TACCATCGCC CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33929
Clone ID Related Accession (Same CDS sequence) XM_006538457.3 , XM_006538457.4
Accession Version XM_006538457.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product phosphatase and actin regulator 4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538457.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG ATGGTGAGGA TTCAGGAAAG 
CTGAGCCATG CTGCATTAAA GAACGGACAC ACCACCCCCA TAGGCAGTGC CAGGTCCTCT 
AGCCCAGTCC TGGTAGAAGA AGAGCCAGAG AGAAGTCTCA GGAACCTCAC TCCAGAAGAG 
GAGTCAAAGA AGAGACTTGG CTCAACTGGA AGCCAGCCTA ACTCTGAAGC CGAGCCTGGT 
CCTGAGCATG CACCTAAGCA GCCTCTGCTG CCCCCCAAGA GACCCTTGTC CTCTTCTTGT 
GAAGCAAAGG AGGTTCCGGC TGGCAGTACA GCAAGGTCCG TCTCCTCCAC CTCTGGCTCT 
ACCACCGTGA CATCTGCTGC CACCACTGCT GCCACAGACA TGACAAAGAC TGTTAAGTCC 
TTTGTGGGCC CCACCCCCGC TCCTGCCCCC GCCCCCAGGA CTCTGCCTGC TGCTCCTGCC 
AGTGCTAACA CTGCTGCCAC CACTACAGCC CCTGCCAAGC AACCCCCCAT CCCACCCCCT 
AAACCAGCTC AGAGAAACAG CAACCCCATC ATTGCTGAAC TATCCCAAGC AATGAACAGT 
GGCACAGTGC TGTCGAAACC ATCCCCACCC TTGCCACCCA AGAGAGGCAT TCCATCAACT 
TCGATACCCA GCTTGGAGCC TGCTGCTTCG TTCACAACTA AGACAGCAAA TGATCAAAGA 
GAAAAAACTG TCTCTTTGTG TTTGGAACCA CCACTGATAA TCCCGCCTTC GTCCCCATCA 
CCCCCACTGC CTACCCACAT ACCTCCCGAG CCTCCACGGT CCCCTCTAGT GCCTGCTAAG 
ACTTTTCAAA TTGTGCCAGA GGTTGAGTTT TCCTCTTCTT CAGATCTATT CCAGGACATT 
TCCCAGCAGG AGGATCAGAA AACAGAAGTC CCCAAGAAGA TACAGGATCA GAGCTTTGGG 
GAGTCCCATA TACCCTCCAG GCTGCCCCCA CTCCCACTGC ATATCCGAAT TCAGCAGGCC 
CTTACCAGCC CCCTTCCTGT GACCCCTCCC TTGGAAGGCA CTCACAGAGC CCATTCACTG 
CTTTTTGAGA ACAGTGACAG CTTCTCCGAG GACACTGGTA CCCTGGGTCG TACCAGGTCA 
CTTCCCATCA CGATTGAGAT GTTGAAAGTT CCAGATGATG AAGAGGAAGA GCAGACCTGC 
CCGTTTGTTG AAGACGTGAC GTCTACCTCA GCCACTCCTA GCCTACCACT GTGTCTGCGA 
GAGGAAGAGA AGGAGAGTGA CTCAGATTCA GAGGGTCCCA TTAAGTACCG AGATGAAGAA 
GAAGATGATG ATGATGATGA AAGCCATCAG AGTGCCCTGG CCAACAGAGT GAAGAGGAAG 
GACACGCTGG CAATGAAGCT GAGCAGCAGA CCCAGTGAAC CAGAGACCAA CCTGAATTCT 
TGGCCTCGTA AAAGCAAGGA GGAGTGGAAT GAAATACGGC ACCAGATCGG GAACACGCTG 
ATCCGGCGGC TGAGTCAGAG ACCAACAGCA GAAGAATTGG AGCAGCGGAA CATACTACAG 
CCTAAAAATG AAGCTGATCG GCAAGCAGAG AAACGGGAAA TCAAGCGTCG CCTCACTAGA 
AAGCTCAGTC AAAGGCCAAC TGTGGCTGAA CTGCTTGCCA GGAAGATTCT GCGGTTTAAT 
GAGTATGTGG AAGTAACTGA TGCTCATGAC TATGACCGGC GAGCAGACAA ACCATGGACC 
AAACTGACTC CTGCTGACAA GGCTGCCATA AGGAAAGAAT TAAATGAATT TAAGAGCTCG 
GAGATGGAGG TTCATGTAGA CAGCAAACAC TTTACACGCT ACCATCGCCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006538520.1
CDS2046..4079
Translation

Target ORF information:

RefSeq Version XM_006538457.4
Organism Mus musculus(house mouse)
Definition Mus musculus phosphatase and actin regulator 4 (Phactr4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006538457.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
ATGGGCCAAG CTGATGTCTC CAGACCGGTA AATCCAGATG CAGTTGAAGA AGCAGAGCAG 
CCCTCTGGAG ACCCAGGCAT GGGCATGGAC AGTGTGGAAG CAGGGGACAC AACACCTCCC 
ACCAAAAGGA AAAGCAAGTT CTCAGCGTTA GGCAAGATCT TCAAGCCGTG GAAATGGAGG 
AAAAAAAAAA GCAGTGATAA GTTCAAAGAG ACATCAGAAG ATGGTGAGGA TTCAGGAAAG 
CTGAGCCATG CTGCATTAAA GAACGGACAC ACCACCCCCA TAGGCAGTGC CAGGTCCTCT 
AGCCCAGTCC TGGTAGAAGA AGAGCCAGAG AGAAGTCTCA GGAACCTCAC TCCAGAAGAG 
GAGTCAAAGA AGAGACTTGG CTCAACTGGA AGCCAGCCTA ACTCTGAAGC CGAGCCTGGT 
CCTGAGCATG CACCTAAGCA GCCTCTGCTG CCCCCCAAGA GACCCTTGTC CTCTTCTTGT 
GAAGCAAAGG AGGTTCCGGC TGGCAGTACA GCAAGGTCCG TCTCCTCCAC CTCTGGCTCT 
ACCACCGTGA CATCTGCTGC CACCACTGCT GCCACAGACA TGACAAAGAC TGTTAAGTCC 
TTTGTGGGCC CCACCCCCGC TCCTGCCCCC GCCCCCAGGA CTCTGCCTGC TGCTCCTGCC 
AGTGCTAACA CTGCTGCCAC CACTACAGCC CCTGCCAAGC AACCCCCCAT CCCACCCCCT 
AAACCAGCTC AGAGAAACAG CAACCCCATC ATTGCTGAAC TATCCCAAGC AATGAACAGT 
GGCACAGTGC TGTCGAAACC ATCCCCACCC TTGCCACCCA AGAGAGGCAT TCCATCAACT 
TCGATACCCA GCTTGGAGCC TGCTGCTTCG TTCACAACTA AGACAGCAAA TGATCAAAGA 
GAAAAAACTG TCTCTTTGTG TTTGGAACCA CCACTGATAA TCCCGCCTTC GTCCCCATCA 
CCCCCACTGC CTACCCACAT ACCTCCCGAG CCTCCACGGT CCCCTCTAGT GCCTGCTAAG 
ACTTTTCAAA TTGTGCCAGA GGTTGAGTTT TCCTCTTCTT CAGATCTATT CCAGGACATT 
TCCCAGCAGG AGGATCAGAA AACAGAAGTC CCCAAGAAGA TACAGGATCA GAGCTTTGGG 
GAGTCCCATA TACCCTCCAG GCTGCCCCCA CTCCCACTGC ATATCCGAAT TCAGCAGGCC 
CTTACCAGCC CCCTTCCTGT GACCCCTCCC TTGGAAGGCA CTCACAGAGC CCATTCACTG 
CTTTTTGAGA ACAGTGACAG CTTCTCCGAG GACACTGGTA CCCTGGGTCG TACCAGGTCA 
CTTCCCATCA CGATTGAGAT GTTGAAAGTT CCAGATGATG AAGAGGAAGA GCAGACCTGC 
CCGTTTGTTG AAGACGTGAC GTCTACCTCA GCCACTCCTA GCCTACCACT GTGTCTGCGA 
GAGGAAGAGA AGGAGAGTGA CTCAGATTCA GAGGGTCCCA TTAAGTACCG AGATGAAGAA 
GAAGATGATG ATGATGATGA AAGCCATCAG AGTGCCCTGG CCAACAGAGT GAAGAGGAAG 
GACACGCTGG CAATGAAGCT GAGCAGCAGA CCCAGTGAAC CAGAGACCAA CCTGAATTCT 
TGGCCTCGTA AAAGCAAGGA GGAGTGGAAT GAAATACGGC ACCAGATCGG GAACACGCTG 
ATCCGGCGGC TGAGTCAGAG ACCAACAGCA GAAGAATTGG AGCAGCGGAA CATACTACAG 
CCTAAAAATG AAGCTGATCG GCAAGCAGAG AAACGGGAAA TCAAGCGTCG CCTCACTAGA 
AAGCTCAGTC AAAGGCCAAC TGTGGCTGAA CTGCTTGCCA GGAAGATTCT GCGGTTTAAT 
GAGTATGTGG AAGTAACTGA TGCTCATGAC TATGACCGGC GAGCAGACAA ACCATGGACC 
AAACTGACTC CTGCTGACAA GGCTGCCATA AGGAAAGAAT TAAATGAATT TAAGAGCTCG 
GAGATGGAGG TTCATGTAGA CAGCAAACAC TTTACACGCT ACCATCGCCC ATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.
Developmental cell43(3)318-331.e5(2017 11)
Rohacek AM,Bebee TW,Tilton RK,Radens CM,McDermott-Roe C,Peart N,Kaur M,Zaykaner M,Cieply B,Musunuru K,Barash Y,Germiller JA,Krantz ID,Carstens RP,Epstein DJ


book

Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes.
Histochemistry and cell biology142(4)411-9(2014 Oct)
Cho HM,Kim JY,Kim H,Sun W


book

Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool.
Journal of cell science126(Pt 2)497-507(2013 Jan)
Huet G,Rajakyl? EK,Viita T,Skarp KP,Crivaro M,Dopie J,Vartiainen MK


book

Different expression patterns of Phactr family members in normal and injured mouse brain.
Neuroscience22137-46(2012 Sep)
Kim JY,Choi SY,Moon Y,Kim HJ,Chin JH,Kim H,Sun W


book

Phactr4 regulates directional migration of enteric neural crest through PP1, integrin signaling, and cofilin activity.
Genes & development26(1)69-81(2012 Jan)
Zhang Y,Kim TH,Niswander L