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Gas8 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Gas8
Entrez Gene ID 104346
Full Name growth arrest specific 8
Synonyms Gas11
General protein information
Preferred Names

growth arrest specific 8

Names

growth arrest-specific protein 8
GAS-11
GAS-8
beta growth arrest specific protein 11
growth arrest specific 11
growth arrest specific protein 11
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

8

8|8 E1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006530552.1 XP_006530615.1 growth arrest-specific protein 8 isoform X3
XM_030243229.1 XP_030099089.1 dynein regulatory complex subunit 4 isoform X3
XM_006530552.2 XP_006530615.1 dynein regulatory complex subunit 4 isoform X4
XM_011248259.2 XP_011246561.1 growth arrest-specific protein 8 isoform X1
XM_030243228.1 XP_030099088.1 dynein regulatory complex subunit 4 isoform X1
XM_006530551.1 XP_006530614.1 growth arrest-specific protein 8 isoform X2
NM_018855.2 NP_061343.2 dynein regulatory complex subunit 4
XM_006530551.2 XP_006530614.1 dynein regulatory complex subunit 4 isoform X2

Pathways from BioSystems


Homologies


Danio rerio (zebrafish) gas8 NP_955928.1
Drosophila melanogaster (fruit fly) Gas8 NP_570065.2
Homo sapiens (human) GAS8 NP_001472.1
Canis lupus familiaris (dog) GAS8 XP_005620585.1
Mus musculus (house mouse) Gas8 NP_061343.2
Gallus gallus (chicken) GAS8 NP_001264546.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005850 XP_001237728.2
Pan troglodytes (chimpanzee) GAS8 XP_511182.2
Xenopus tropicalis (tropical clawed frog) gas8 NP_001106441.1
GAS8 XP_001093935.1
Bos taurus (cattle) GAS8 NP_001091038.1
Rattus norvegicus (Norway rat) Gas8 NP_001034119.1

Gene Ontology


Bibliography

Related articles in PubMed

A mutation in
Abdelhamed Z, Vuong SM, Hill L, Shula C, Timms A, Beier D, Campbell K, Mangano FT, Stottmann RW, Goto J
Development (Cambridge, England)145(1)(2018 Jan)

Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease.
Lewis WR, Malarkey EB, Tritschler D, Bower R, Pasek RC, Porath JD, Birket SE, Saunier S, Antignac C, Knowles MR, Leigh MW, Zariwala MA, Challa AK, Kesterson RA, Rowe SM, Drummond IA, Parant JM, Hildebrandt F, Porter ME, Yoder BK, Berbari NF
PLoS genetics12(7)e1006220(2016 07)

Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
Evron T, Philipp M, Lu J, Meloni AR, Burkhalter M, Chen W, Caron MG
The Journal of biological chemistry286(31)27676-86(2011 Aug)

Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells.
Nishimura N, Araki K, Shinahara W, Nakano Y, Nishimura K, Higashio H, Sasaki T
Archives of biochemistry and biophysics474(1)136-42(2008 Jun)

Direct interaction of Gas11 with microtubules: implications for the dynein regulatory complex.
Bekker JM, Colantonio JR, Stephens AD, Clarke WT, King SJ, Hill KL, Crosbie RH
Cell motility and the cytoskeleton64(6)461-73(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, we generate the first mouse with a Gas8 mutation and show that it causes severe Primary Ciliary Dyskinesia (PCD)phenotypes; however, there were no overt Hh pathway phenotypes. In addition, we identified two human patients with missense variants in Gas8
Title: Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease.

results identify Gas8 as a positive regulator of Hh signaling that cooperates with GRK2 to control Smo signaling
Title: Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.

Gas8 was specifically interacted with the GTP-bound form of Rab3B and co-localized with Rab3B at the Golgi in NIH 3T3 cells
Title: Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells.

Gas11, the mammalian trypanin/PF2 homologue, associates with microtubules in vitro and in vivo.
Title: Direct interaction of Gas11 with microtubules: implications for the dynein regulatory complex.

a growth arrest specific gene regulated during male gametogensis
Title: Isolation and properties of Gas8, a growth arrest-specific gene regulated during male gametogenesis to produce a protein associated with the sperm motility apparatus.

The following Gas8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gas8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu72083 XM_011248259.2
Latest version!		 Mus musculus growth arrest specific 8 (Gas8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu11098 NM_018855.2
Latest version!		 Mus musculus growth arrest specific 8 (Gas8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OMu41138 XM_006530551.2
Latest version!		 Mus musculus growth arrest specific 8 (Gas8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu41139 XM_006530552.2
Latest version!		 Mus musculus growth arrest specific 8 (Gas8), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu89728 XM_030243228.1
Latest version!		 Mus musculus growth arrest specific 8 (Gas8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu89729 XM_030243229.1
Latest version!		 Mus musculus growth arrest specific 8 (Gas8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu41139 XM_006530552.1 Mus musculus growth arrest specific 8 (Gas8), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu41138 XM_006530551.1 Mus musculus growth arrest specific 8 (Gas8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu41139
Clone ID Related Accession (Same CDS sequence) XM_006530552.1 , XM_006530552.2
Accession Version XM_006530552.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product growth arrest-specific protein 8 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

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481
541
601
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721
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841
ATGAAGATGC TTAGAGACGA ACTTGACCTG CGGAGGAAGA CTGAGATCCA TGAGGTAGAG 
GAGAGGAAAA ACGGCCAGAT CAGCACTCTG ATGCAGCGCC ACGAGGAGGC CTTCACGGAC 
ATCAAGAACT ACTACAACGA CATCACTCTC AACAACCTGG CCCTCATCAA CTCCCTCAAG 
GAGCAGATGG AGGACATGCG TAAGAAGGAG GAACACATGG AGAGGGAGAT GGCGGAGGTG 
ACTCTGCAGA ACCGGCGGCT GGCCGACCCG CTGCAGAAAG CCAAGGACGA GATGAACGAG 
ATGCAGAAGA GACTGGGGAA CCACGAGAGG GACAAGCAGA TTCTGGTCTG TACAAAGGCA 
CGTTTGAAGG TGGCTGAGAG GGAGCTAAAG GACCTGAAAT GGGAACATGA GGTCCTGGAG 
CAGAGGTTCA TCAAGGTGCA GCAGGAGAGG GAAGAGCTCT ATCGGAAGTT TGCAGACGCC 
ATCCAGGAGG TGCAGCAGAA GACGGGCTTC AAGAACCTGC TGCTGGAGCG GAAGCTGCAG 
GCCCTGAACG CTGCTGTGGA GAAGAGAGAG GTTCAGTTCA ATGAGGTGCT GGCCGCCTCC 
AACCTGGACC CCACAGCATT AACGCTTGTG TCCCGCAAAC TTGAGGATGT TCTAGAATCG 
AAGAATACCA CCATCAAGGA CTTACAGTAT GAGCTGGCCC GGGTCTGCAA GGCCCACAAT 
GACCTGCTGC GTACATATGA GGCGAAGTTA CTGGCCTTCG GGATCCCCCT GGACAATGTG 
GGGTTCAAGC CCCTGGAGAC AGCAGTGATT GGACAGACAC TGGGACAGGG CCCTGCAGGA 
CTTGTGGGTG CCCCTACATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006530615.1
CDS117..977
Translation

Target ORF information:

RefSeq Version XM_006530552.1
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006530552.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAAGATGC TTAGAGACGA ACTTGACCTG CGGAGGAAGA CTGAGATCCA TGAGGTAGAG 
GAGAGGAAAA ACGGCCAGAT CAGCACTCTG ATGCAGCGCC ACGAGGAGGC CTTCACGGAC 
ATCAAGAACT ACTACAACGA CATCACTCTC AACAACCTGG CCCTCATCAA CTCCCTCAAG 
GAGCAGATGG AGGACATGCG TAAGAAGGAG GAACACATGG AGAGGGAGAT GGCGGAGGTG 
ACTCTGCAGA ACCGGCGGCT GGCCGACCCG CTGCAGAAAG CCAAGGACGA GATGAACGAG 
ATGCAGAAGA GACTGGGGAA CCACGAGAGG GACAAGCAGA TTCTGGTCTG TACAAAGGCA 
CGTTTGAAGG TGGCTGAGAG GGAGCTAAAG GACCTGAAAT GGGAACATGA GGTCCTGGAG 
CAGAGGTTCA TCAAGGTGCA GCAGGAGAGG GAAGAGCTCT ATCGGAAGTT TGCAGACGCC 
ATCCAGGAGG TGCAGCAGAA GACGGGCTTC AAGAACCTGC TGCTGGAGCG GAAGCTGCAG 
GCCCTGAACG CTGCTGTGGA GAAGAGAGAG GTTCAGTTCA ATGAGGTGCT GGCCGCCTCC 
AACCTGGACC CCACAGCATT AACGCTTGTG TCCCGCAAAC TTGAGGATGT TCTAGAATCG 
AAGAATACCA CCATCAAGGA CTTACAGTAT GAGCTGGCCC GGGTCTGCAA GGCCCACAAT 
GACCTGCTGC GTACATATGA GGCGAAGTTA CTGGCCTTCG GGATCCCCCT GGACAATGTG 
GGGTTCAAGC CCCTGGAGAC AGCAGTGATT GGACAGACAC TGGGACAGGG CCCTGCAGGA 
CTTGTGGGTG CCCCTACATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu41138
Clone ID Related Accession (Same CDS sequence) XM_006530551.1 , XM_006530551.2
Accession Version XM_006530551.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 900bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product growth arrest-specific protein 8 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATTGA GGCCAAGTAT GATAAGAAGA TGAAGATGCT TAGAGACGAA 
CTTGACCTGC GGAGGAAGAC TGAGATCCAT GAGGTAGAGG AGAGGAAAAA CGGCCAGATC 
AGCACTCTGA TGCAGCGCCA CGAGGAGGCC TTCACGGACA TCAAGAACTA CTACAACGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAC TCCCTCAAGG AGCAGATGGA GGACATGCGT 
AAGAAGGAGG AACACATGGA GAGGGAGATG GCGGAGGTGA CTCTGCAGAA CCGGCGGCTG 
GCCGACCCGC TGCAGAAAGC CAAGGACGAG ATGAACGAGA TGCAGAAGAG ACTGGGGAAC 
CACGAGAGGG ACAAGCAGAT TCTGGTCTGT ACAAAGGCAC GTTTGAAGGT GGCTGAGAGG 
GAGCTAAAGG ACCTGAAATG GGAACATGAG GTCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTT GCAGACGCCA TCCAGGAGGT GCAGCAGAAG 
ACGGGCTTCA AGAACCTGCT GCTGGAGCGG AAGCTGCAGG CCCTGAACGC TGCTGTGGAG 
AAGAGAGAGG TTCAGTTCAA TGAGGTGCTG GCCGCCTCCA ACCTGGACCC CACAGCATTA 
ACGCTTGTGT CCCGCAAACT TGAGGATGTT CTAGAATCGA AGAATACCAC CATCAAGGAC 
TTACAGTATG AGCTGGCCCG GGTCTGCAAG GCCCACAATG ACCTGCTGCG TACATATGAG 
GCGAAGTTAC TGGCCTTCGG GATCCCCCTG GACAATGTGG GGTTCAAGCC CCTGGAGACA 
GCAGTGATTG GACAGACACT GGGACAGGGC CCTGCAGGAC TTGTGGGTGC CCCTACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006530614.1
CDS542..1441
Translation

Target ORF information:

RefSeq Version XM_006530551.1
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006530551.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATTGA GGCCAAGTAT GATAAGAAGA TGAAGATGCT TAGAGACGAA 
CTTGACCTGC GGAGGAAGAC TGAGATCCAT GAGGTAGAGG AGAGGAAAAA CGGCCAGATC 
AGCACTCTGA TGCAGCGCCA CGAGGAGGCC TTCACGGACA TCAAGAACTA CTACAACGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAC TCCCTCAAGG AGCAGATGGA GGACATGCGT 
AAGAAGGAGG AACACATGGA GAGGGAGATG GCGGAGGTGA CTCTGCAGAA CCGGCGGCTG 
GCCGACCCGC TGCAGAAAGC CAAGGACGAG ATGAACGAGA TGCAGAAGAG ACTGGGGAAC 
CACGAGAGGG ACAAGCAGAT TCTGGTCTGT ACAAAGGCAC GTTTGAAGGT GGCTGAGAGG 
GAGCTAAAGG ACCTGAAATG GGAACATGAG GTCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTT GCAGACGCCA TCCAGGAGGT GCAGCAGAAG 
ACGGGCTTCA AGAACCTGCT GCTGGAGCGG AAGCTGCAGG CCCTGAACGC TGCTGTGGAG 
AAGAGAGAGG TTCAGTTCAA TGAGGTGCTG GCCGCCTCCA ACCTGGACCC CACAGCATTA 
ACGCTTGTGT CCCGCAAACT TGAGGATGTT CTAGAATCGA AGAATACCAC CATCAAGGAC 
TTACAGTATG AGCTGGCCCG GGTCTGCAAG GCCCACAATG ACCTGCTGCG TACATATGAG 
GCGAAGTTAC TGGCCTTCGG GATCCCCCTG GACAATGTGG GGTTCAAGCC CCTGGAGACA 
GCAGTGATTG GACAGACACT GGGACAGGGC CCTGCAGGAC TTGTGGGTGC CCCTACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu72083
Clone ID Related Accession (Same CDS sequence) XM_011248259.2
Accession Version XM_011248259.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1083bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product growth arrest-specific protein 8 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011248259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCACCCA AAAAGAAAGG GAAGAAGGGG AAAGCCAAAG GGACCGCGAT TGTTGATGGC 
GTCGCACCAG AGGACATGAC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACA GGGAGAGAGA GGAGCGTAAC TACTTCCAGC TGGAGCGCGA CAAGATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT ATACAAGCAG 
AAGGTGAAGC ACCTGCTGTA CGAGCACCAG AACAACTTGG CTGAGGTGAA GGCTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCACCC AGGAGGGCGC TCTGCGCAAG 
GACATGCGGG TGCTGAAGGT GGAGCTCAAG GAGCAGGAGC TGGCTAACGA GGTGGTCATA 
AAGAACCTGT GCCTGAAACA AGCCGAGGAG ATCACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATTGAGGC CAAGTATGAT AAGAAGATGA AGATGCTTAG AGACGAACTT 
GACCTGCGGA GGAAGACTGA GATCCATGAG GTAGAGGAGA GGAAAAACGG CCAGATCAGC 
ACTCTGATGC AGCGCCACGA GGAGGCCTTC ACGGACATCA AGAACTACTA CAACGACATC 
ACTCTCAACA ACCTGGCCCT CATCAACTCC CTCAAGGAGC AGATGGAGGA CATGCGTAAG 
AAGGAGGAAC ACATGGAGAG GGAGATGGCG GAGGTGACTC TGCAGAACCG GCGGCTGGCC 
GACCCGCTGC AGAAAGCCAA GGACGAGATG AACGAGATGC AGAAGAGACT GGGGAACCAC 
GAGAGGGACA AGCAGATTCT GGTCTGTACA AAGGCACGTT TGAAGGTGGC TGAGAGGGAG 
CTAAAGGACC TGAAATGGGA ACATGAGGTC CTGGAGCAGA GGTTCATCAA GGGTCTCACC 
GTCCCATGTG GCCAGGCCTT ACTCCTGGGA CCCTGCCTCC TCCTGCTCCA GCCTTCTGAG 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011246561.1
CDS106..1188
Translation

Target ORF information:

RefSeq Version XM_011248259.2
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011248259.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCACCCA AAAAGAAAGG GAAGAAGGGG AAAGCCAAAG GGACCGCGAT TGTTGATGGC 
GTCGCACCAG AGGACATGAC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACA GGGAGAGAGA GGAGCGTAAC TACTTCCAGC TGGAGCGCGA CAAGATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT ATACAAGCAG 
AAGGTGAAGC ACCTGCTGTA CGAGCACCAG AACAACTTGG CTGAGGTGAA GGCTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCACCC AGGAGGGCGC TCTGCGCAAG 
GACATGCGGG TGCTGAAGGT GGAGCTCAAG GAGCAGGAGC TGGCTAACGA GGTGGTCATA 
AAGAACCTGT GCCTGAAACA AGCCGAGGAG ATCACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATTGAGGC CAAGTATGAT AAGAAGATGA AGATGCTTAG AGACGAACTT 
GACCTGCGGA GGAAGACTGA GATCCATGAG GTAGAGGAGA GGAAAAACGG CCAGATCAGC 
ACTCTGATGC AGCGCCACGA GGAGGCCTTC ACGGACATCA AGAACTACTA CAACGACATC 
ACTCTCAACA ACCTGGCCCT CATCAACTCC CTCAAGGAGC AGATGGAGGA CATGCGTAAG 
AAGGAGGAAC ACATGGAGAG GGAGATGGCG GAGGTGACTC TGCAGAACCG GCGGCTGGCC 
GACCCGCTGC AGAAAGCCAA GGACGAGATG AACGAGATGC AGAAGAGACT GGGGAACCAC 
GAGAGGGACA AGCAGATTCT GGTCTGTACA AAGGCACGTT TGAAGGTGGC TGAGAGGGAG 
CTAAAGGACC TGAAATGGGA ACATGAGGTC CTGGAGCAGA GGTTCATCAA GGGTCTCACC 
GTCCCATGTG GCCAGGCCTT ACTCCTGGGA CCCTGCCTCC TCCTGCTCCA GCCTTCTGAG 
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu11098
Clone ID Related Accession (Same CDS sequence) NM_018855.2
Accession Version NM_018855.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-05
Organism Mus musculus(house mouse)
Product dynein regulatory complex subunit 4
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158362.5 and AY692443.1. On Feb 24, 2007 this sequence version replaced NM_018855.1. ##Evidence-Data-START## Transcript exon combination :: AY692443.1, U19859.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCACCCA AAAAGAAAGG GAAGAAGGGG AAAGCCAAAG GGACCGCGAT TGTTGATGGC 
GTCGCACCAG AGGACATGAC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACA GGGAGAGAGA GGAGCGTAAC TACTTCCAGC TGGAGCGCGA CAAGATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT ATACAAGCAG 
AAGGTGAAGC ACCTGCTGTA CGAGCACCAG AACAACTTGG CTGAGGTGAA GGCTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCACCC AGGAGGGCGC TCTGCGCAAG 
GACATGCGGG TGCTGAAGGT GGAGCTCAAG GAGCAGGAGC TGGCTAACGA GGTGGTCATA 
AAGAACCTGT GCCTGAAACA AGCCGAGGAG ATCACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATTGAGGC CAAGTATGAT AAGAAGATGA AGATGCTTAG AGACGAACTT 
GACCTGCGGA GGAAGACTGA GATCCATGAG GTAGAGGAGA GGAAAAACGG CCAGATCAGC 
ACTCTGATGC AGCGCCACGA GGAGGCCTTC ACGGACATCA AGAACTACTA CAACGACATC 
ACTCTCAACA ACCTGGCCCT CATCAACTCC CTCAAGGAGC AGATGGAGGA CATGCGTAAG 
AAGGAGGAAC ACATGGAGAG GGAGATGGCG GAGGTGACTC TGCAGAACCG GCGGCTGGCC 
GACCCGCTGC AGAAAGCCAA GGACGAGATG AACGAGATGC AGAAGAGACT GGGGAACCAC 
GAGAGGGACA AGCAGATTCT GGTCTGTACA AAGGCACGTT TGAAGGTGGC TGAGAGGGAG 
CTAAAGGACC TGAAATGGGA ACATGAGGTC CTGGAGCAGA GGTTCATCAA GGTGCAGCAG 
GAGAGGGAAG AGCTCTATCG GAAGTTTGCA GACGCCATCC AGGAGGTGCA GCAGAAGACG 
GGCTTCAAGA ACCTGCTGCT GGAGCGGAAG CTGCAGGCCC TGAACGCTGC TGTGGAGAAG 
AGAGAGGTTC AGTTCAATGA GGTGCTGGCC GCCTCCAACC TGGACCCCAC AGCATTAACG 
CTTGTGTCCC GCAAACTTGA GGATGTTCTA GAATCGAAGA ATACCACCAT CAAGGACTTA 
CAGTATGAGC TGGCCCGGGT CTGCAAGGCC CACAATGACC TGCTGCGTAC ATATGAGGCG 
AAGTTACTGG CCTTCGGGAT CCCCCTGGAC AATGTGGGGT TCAAGCCCCT GGAGACAGCA 
GTGATTGGAC AGACACTGGG ACAGGGCCCT GCAGGACTTG TGGGTGCCCC TACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_061343.2
CDS116..1552
Translation

Target ORF information:

RefSeq Version NM_018855.2
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_018855.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCACCCA AAAAGAAAGG GAAGAAGGGG AAAGCCAAAG GGACCGCGAT TGTTGATGGC 
GTCGCACCAG AGGACATGAC CAAGGAGCAG GTGGAGGAAC ATGTCGCCCG CATCCGGGAG 
GAGCTGGACA GGGAGAGAGA GGAGCGTAAC TACTTCCAGC TGGAGCGCGA CAAGATCCAC 
ACCTTCTGGG AGATCACGCG GAGGCAGCTG GAGGAGAAGA AGGCTGAGCT GCGCAACAAA 
GACCGGGAGA TGGAGGAGGC CGAGGAGAGG CACCAGGTGG AGATCAAGGT ATACAAGCAG 
AAGGTGAAGC ACCTGCTGTA CGAGCACCAG AACAACTTGG CTGAGGTGAA GGCTGAGGGC 
ACCGTGGTCA TGAAGCTGGC CCAGAAGGAG CACCGCACCC AGGAGGGCGC TCTGCGCAAG 
GACATGCGGG TGCTGAAGGT GGAGCTCAAG GAGCAGGAGC TGGCTAACGA GGTGGTCATA 
AAGAACCTGT GCCTGAAACA AGCCGAGGAG ATCACCAAGA TGCGAAATGA CTTTGAAAGG 
CAAGTTCGAG AAATTGAGGC CAAGTATGAT AAGAAGATGA AGATGCTTAG AGACGAACTT 
GACCTGCGGA GGAAGACTGA GATCCATGAG GTAGAGGAGA GGAAAAACGG CCAGATCAGC 
ACTCTGATGC AGCGCCACGA GGAGGCCTTC ACGGACATCA AGAACTACTA CAACGACATC 
ACTCTCAACA ACCTGGCCCT CATCAACTCC CTCAAGGAGC AGATGGAGGA CATGCGTAAG 
AAGGAGGAAC ACATGGAGAG GGAGATGGCG GAGGTGACTC TGCAGAACCG GCGGCTGGCC 
GACCCGCTGC AGAAAGCCAA GGACGAGATG AACGAGATGC AGAAGAGACT GGGGAACCAC 
GAGAGGGACA AGCAGATTCT GGTCTGTACA AAGGCACGTT TGAAGGTGGC TGAGAGGGAG 
CTAAAGGACC TGAAATGGGA ACATGAGGTC CTGGAGCAGA GGTTCATCAA GGTGCAGCAG 
GAGAGGGAAG AGCTCTATCG GAAGTTTGCA GACGCCATCC AGGAGGTGCA GCAGAAGACG 
GGCTTCAAGA ACCTGCTGCT GGAGCGGAAG CTGCAGGCCC TGAACGCTGC TGTGGAGAAG 
AGAGAGGTTC AGTTCAATGA GGTGCTGGCC GCCTCCAACC TGGACCCCAC AGCATTAACG 
CTTGTGTCCC GCAAACTTGA GGATGTTCTA GAATCGAAGA ATACCACCAT CAAGGACTTA 
CAGTATGAGC TGGCCCGGGT CTGCAAGGCC CACAATGACC TGCTGCGTAC ATATGAGGCG 
AAGTTACTGG CCTTCGGGAT CCCCCTGGAC AATGTGGGGT TCAAGCCCCT GGAGACAGCA 
GTGATTGGAC AGACACTGGG ACAGGGCCCT GCAGGACTTG TGGGTGCCCC TACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu41138
Clone ID Related Accession (Same CDS sequence) XM_006530551.1 , XM_006530551.2
Accession Version XM_006530551.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 900bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product dynein regulatory complex subunit 4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATTGA GGCCAAGTAT GATAAGAAGA TGAAGATGCT TAGAGACGAA 
CTTGACCTGC GGAGGAAGAC TGAGATCCAT GAGGTAGAGG AGAGGAAAAA CGGCCAGATC 
AGCACTCTGA TGCAGCGCCA CGAGGAGGCC TTCACGGACA TCAAGAACTA CTACAACGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAC TCCCTCAAGG AGCAGATGGA GGACATGCGT 
AAGAAGGAGG AACACATGGA GAGGGAGATG GCGGAGGTGA CTCTGCAGAA CCGGCGGCTG 
GCCGACCCGC TGCAGAAAGC CAAGGACGAG ATGAACGAGA TGCAGAAGAG ACTGGGGAAC 
CACGAGAGGG ACAAGCAGAT TCTGGTCTGT ACAAAGGCAC GTTTGAAGGT GGCTGAGAGG 
GAGCTAAAGG ACCTGAAATG GGAACATGAG GTCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTT GCAGACGCCA TCCAGGAGGT GCAGCAGAAG 
ACGGGCTTCA AGAACCTGCT GCTGGAGCGG AAGCTGCAGG CCCTGAACGC TGCTGTGGAG 
AAGAGAGAGG TTCAGTTCAA TGAGGTGCTG GCCGCCTCCA ACCTGGACCC CACAGCATTA 
ACGCTTGTGT CCCGCAAACT TGAGGATGTT CTAGAATCGA AGAATACCAC CATCAAGGAC 
TTACAGTATG AGCTGGCCCG GGTCTGCAAG GCCCACAATG ACCTGCTGCG TACATATGAG 
GCGAAGTTAC TGGCCTTCGG GATCCCCCTG GACAATGTGG GGTTCAAGCC CCTGGAGACA 
GCAGTGATTG GACAGACACT GGGACAGGGC CCTGCAGGAC TTGTGGGTGC CCCTACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006530614.1
CDS616..1515
Translation

Target ORF information:

RefSeq Version XM_006530551.2
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006530551.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATTGA GGCCAAGTAT GATAAGAAGA TGAAGATGCT TAGAGACGAA 
CTTGACCTGC GGAGGAAGAC TGAGATCCAT GAGGTAGAGG AGAGGAAAAA CGGCCAGATC 
AGCACTCTGA TGCAGCGCCA CGAGGAGGCC TTCACGGACA TCAAGAACTA CTACAACGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAC TCCCTCAAGG AGCAGATGGA GGACATGCGT 
AAGAAGGAGG AACACATGGA GAGGGAGATG GCGGAGGTGA CTCTGCAGAA CCGGCGGCTG 
GCCGACCCGC TGCAGAAAGC CAAGGACGAG ATGAACGAGA TGCAGAAGAG ACTGGGGAAC 
CACGAGAGGG ACAAGCAGAT TCTGGTCTGT ACAAAGGCAC GTTTGAAGGT GGCTGAGAGG 
GAGCTAAAGG ACCTGAAATG GGAACATGAG GTCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTT GCAGACGCCA TCCAGGAGGT GCAGCAGAAG 
ACGGGCTTCA AGAACCTGCT GCTGGAGCGG AAGCTGCAGG CCCTGAACGC TGCTGTGGAG 
AAGAGAGAGG TTCAGTTCAA TGAGGTGCTG GCCGCCTCCA ACCTGGACCC CACAGCATTA 
ACGCTTGTGT CCCGCAAACT TGAGGATGTT CTAGAATCGA AGAATACCAC CATCAAGGAC 
TTACAGTATG AGCTGGCCCG GGTCTGCAAG GCCCACAATG ACCTGCTGCG TACATATGAG 
GCGAAGTTAC TGGCCTTCGG GATCCCCCTG GACAATGTGG GGTTCAAGCC CCTGGAGACA 
GCAGTGATTG GACAGACACT GGGACAGGGC CCTGCAGGAC TTGTGGGTGC CCCTACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu41139
Clone ID Related Accession (Same CDS sequence) XM_006530552.1 , XM_006530552.2
Accession Version XM_006530552.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product dynein regulatory complex subunit 4 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAAGATGC TTAGAGACGA ACTTGACCTG CGGAGGAAGA CTGAGATCCA TGAGGTAGAG 
GAGAGGAAAA ACGGCCAGAT CAGCACTCTG ATGCAGCGCC ACGAGGAGGC CTTCACGGAC 
ATCAAGAACT ACTACAACGA CATCACTCTC AACAACCTGG CCCTCATCAA CTCCCTCAAG 
GAGCAGATGG AGGACATGCG TAAGAAGGAG GAACACATGG AGAGGGAGAT GGCGGAGGTG 
ACTCTGCAGA ACCGGCGGCT GGCCGACCCG CTGCAGAAAG CCAAGGACGA GATGAACGAG 
ATGCAGAAGA GACTGGGGAA CCACGAGAGG GACAAGCAGA TTCTGGTCTG TACAAAGGCA 
CGTTTGAAGG TGGCTGAGAG GGAGCTAAAG GACCTGAAAT GGGAACATGA GGTCCTGGAG 
CAGAGGTTCA TCAAGGTGCA GCAGGAGAGG GAAGAGCTCT ATCGGAAGTT TGCAGACGCC 
ATCCAGGAGG TGCAGCAGAA GACGGGCTTC AAGAACCTGC TGCTGGAGCG GAAGCTGCAG 
GCCCTGAACG CTGCTGTGGA GAAGAGAGAG GTTCAGTTCA ATGAGGTGCT GGCCGCCTCC 
AACCTGGACC CCACAGCATT AACGCTTGTG TCCCGCAAAC TTGAGGATGT TCTAGAATCG 
AAGAATACCA CCATCAAGGA CTTACAGTAT GAGCTGGCCC GGGTCTGCAA GGCCCACAAT 
GACCTGCTGC GTACATATGA GGCGAAGTTA CTGGCCTTCG GGATCCCCCT GGACAATGTG 
GGGTTCAAGC CCCTGGAGAC AGCAGTGATT GGACAGACAC TGGGACAGGG CCCTGCAGGA 
CTTGTGGGTG CCCCTACATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006530615.1
CDS117..977
Translation

Target ORF information:

RefSeq Version XM_006530552.2
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006530552.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGAAGATGC TTAGAGACGA ACTTGACCTG CGGAGGAAGA CTGAGATCCA TGAGGTAGAG 
GAGAGGAAAA ACGGCCAGAT CAGCACTCTG ATGCAGCGCC ACGAGGAGGC CTTCACGGAC 
ATCAAGAACT ACTACAACGA CATCACTCTC AACAACCTGG CCCTCATCAA CTCCCTCAAG 
GAGCAGATGG AGGACATGCG TAAGAAGGAG GAACACATGG AGAGGGAGAT GGCGGAGGTG 
ACTCTGCAGA ACCGGCGGCT GGCCGACCCG CTGCAGAAAG CCAAGGACGA GATGAACGAG 
ATGCAGAAGA GACTGGGGAA CCACGAGAGG GACAAGCAGA TTCTGGTCTG TACAAAGGCA 
CGTTTGAAGG TGGCTGAGAG GGAGCTAAAG GACCTGAAAT GGGAACATGA GGTCCTGGAG 
CAGAGGTTCA TCAAGGTGCA GCAGGAGAGG GAAGAGCTCT ATCGGAAGTT TGCAGACGCC 
ATCCAGGAGG TGCAGCAGAA GACGGGCTTC AAGAACCTGC TGCTGGAGCG GAAGCTGCAG 
GCCCTGAACG CTGCTGTGGA GAAGAGAGAG GTTCAGTTCA ATGAGGTGCT GGCCGCCTCC 
AACCTGGACC CCACAGCATT AACGCTTGTG TCCCGCAAAC TTGAGGATGT TCTAGAATCG 
AAGAATACCA CCATCAAGGA CTTACAGTAT GAGCTGGCCC GGGTCTGCAA GGCCCACAAT 
GACCTGCTGC GTACATATGA GGCGAAGTTA CTGGCCTTCG GGATCCCCCT GGACAATGTG 
GGGTTCAAGC CCCTGGAGAC AGCAGTGATT GGACAGACAC TGGGACAGGG CCCTGCAGGA 
CTTGTGGGTG CCCCTACATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu89728
Clone ID Related Accession (Same CDS sequence) XM_030243228.1
Accession Version XM_030243228.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product dynein regulatory complex subunit 4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAAGCTGG CCCAGAAGGA GCACCGCACC CAGGAGGGCG CTCTGCGCAA GGACATGCGG 
GTGCTGAAGG TGGAGCTCAA GGAGCAGGAG CTGGCTAACG AGGTGGTCAT AAAGAACCTG 
TGCCTGAAAC AAGCCGAGGA GATCACCAAG ATGCGAAATG ACTTTGAAAG GCAAGTTCGA 
GAAATTGAGG CCAAGTATGA TAAGAAGATG AAGATGCTTA GAGACGAACT TGACCTGCGG 
AGGAAGACTG AGATCCATGA GGTAGAGGAG AGGAAAAACG GCCAGATCAG CACTCTGATG 
CAGCGCCACG AGGAGGCCTT CACGGACATC AAGAACTACT ACAACGACAT CACTCTCAAC 
AACCTGGCCC TCATCAACTC CCTCAAGGAG CAGATGGAGG ACATGCGTAA GAAGGAGGAA 
CACATGGAGA GGGAGATGGC GGAGGTGACT CTGCAGAACC GGCGGCTGGC CGACCCGCTG 
CAGAAAGCCA AGGACGAGAT GAACGAGATG CAGAAGAGAC TGGGGAACCA CGAGAGGGAC 
AAGCAGATTC TGGTCTGTAC AAAGGCACGT TTGAAGGTGG CTGAGAGGGA GCTAAAGGAC 
CTGAAATGGG AACATGAGGT CCTGGAGCAG AGGTTCATCA AGGTGCAGCA GGAGAGGGAA 
GAGCTCTATC GGAAGTTTGC AGACGCCATC CAGGAGGTGC AGCAGAAGAC GGGCTTCAAG 
AACCTGCTGC TGGAGCGGAA GCTGCAGGCC CTGAACGCTG CTGTGGAGAA GAGAGAGGTT 
CAGTTCAATG AGGTGCTGGC CGCCTCCAAC CTGGACCCCA CAGCATTAAC GCTTGTGTCC 
CGCAAACTTG AGGATGTTCT AGAATCGAAG AATACCACCA TCAAGGACTT ACAGTATGAG 
CTGGCCCGGG TCTGCAAGGC CCACAATGAC CTGCTGCGTA CATATGAGGC GAAGTTACTG 
GCCTTCGGGA TCCCCCTGGA CAATGTGGGG TTCAAGCCCC TGGAGACAGC AGTGATTGGA 
CAGACACTGG GACAGGGCCC TGCAGGACTT GTGGGTGCCC CTACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030099088.1
CDS509..1576
Translation

Target ORF information:

RefSeq Version XM_030243228.1
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030243228.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAAGCTGG CCCAGAAGGA GCACCGCACC CAGGAGGGCG CTCTGCGCAA GGACATGCGG 
GTGCTGAAGG TGGAGCTCAA GGAGCAGGAG CTGGCTAACG AGGTGGTCAT AAAGAACCTG 
TGCCTGAAAC AAGCCGAGGA GATCACCAAG ATGCGAAATG ACTTTGAAAG GCAAGTTCGA 
GAAATTGAGG CCAAGTATGA TAAGAAGATG AAGATGCTTA GAGACGAACT TGACCTGCGG 
AGGAAGACTG AGATCCATGA GGTAGAGGAG AGGAAAAACG GCCAGATCAG CACTCTGATG 
CAGCGCCACG AGGAGGCCTT CACGGACATC AAGAACTACT ACAACGACAT CACTCTCAAC 
AACCTGGCCC TCATCAACTC CCTCAAGGAG CAGATGGAGG ACATGCGTAA GAAGGAGGAA 
CACATGGAGA GGGAGATGGC GGAGGTGACT CTGCAGAACC GGCGGCTGGC CGACCCGCTG 
CAGAAAGCCA AGGACGAGAT GAACGAGATG CAGAAGAGAC TGGGGAACCA CGAGAGGGAC 
AAGCAGATTC TGGTCTGTAC AAAGGCACGT TTGAAGGTGG CTGAGAGGGA GCTAAAGGAC 
CTGAAATGGG AACATGAGGT CCTGGAGCAG AGGTTCATCA AGGTGCAGCA GGAGAGGGAA 
GAGCTCTATC GGAAGTTTGC AGACGCCATC CAGGAGGTGC AGCAGAAGAC GGGCTTCAAG 
AACCTGCTGC TGGAGCGGAA GCTGCAGGCC CTGAACGCTG CTGTGGAGAA GAGAGAGGTT 
CAGTTCAATG AGGTGCTGGC CGCCTCCAAC CTGGACCCCA CAGCATTAAC GCTTGTGTCC 
CGCAAACTTG AGGATGTTCT AGAATCGAAG AATACCACCA TCAAGGACTT ACAGTATGAG 
CTGGCCCGGG TCTGCAAGGC CCACAATGAC CTGCTGCGTA CATATGAGGC GAAGTTACTG 
GCCTTCGGGA TCCCCCTGGA CAATGTGGGG TTCAAGCCCC TGGAGACAGC AGTGATTGGA 
CAGACACTGG GACAGGGCCC TGCAGGACTT GTGGGTGCCC CTACATAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu89729
Clone ID Related Accession (Same CDS sequence) XM_030243229.1
Accession Version XM_030243229.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product dynein regulatory complex subunit 4 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATTGA GGCCAAGTAT GATAAGAAGA TGAAGATGCT TAGAGACGAA 
CTTGACCTGC GGAGGAAGAC TGAGATCCAT GAGGTAGAGG AGAGGAAAAA CGGCCAGATC 
AGCACTCTGA TGCAGCGCCA CGAGGAGGCC TTCACGGACA TCAAGAACTA CTACAACGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAC TCCCTCAAGG AGCAGATGGA GGACATGCGT 
AAGAAGGAGG AACACATGGA GAGGGAGATG GCGGAGGTGA CTCTGCAGAA CCGGCGGCTG 
GCCGACCCGC TGCAGAAAGC CAAGGACGAG ATGAACGAGA TGCAGAAGAG ACTGGGGAAC 
CACGAGAGGG ACAAGCAGAT TCTGGTCTGT ACAAAGGCAC GTTTGAAGGT GGCTGAGAGG 
GAGCTAAAGG ACCTGAAATG GGAACATGAG GTCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTT GCAGACGCCA TCCAGGAGGT GCAGCAGAAG 
ACGGGCTTCA AGAACCTGCT GCTGGAGCGG AAGCTGCAGG CCCTGAACGC TGCTGTGGAG 
AAGAGAGAGG TTCAGTTCAA TGAGGTGCTG GCCGCCTCCA ACCTGGACCC CACAGCATTA 
ACGCTTGTGT CCCGCAAACT TGAGGATGTT CTAGAATCGA AGAATACCAC CATCAAGGAC 
TTACAGTATG AGCTGGCCCG GGTCTGCAAG GTGAGGCTAA TCCCCCCATC CGCAGGGCTC 
CACCTTCTCC TCCCTCAGAG CTGTGCCCTG TGCCCAGCTT CACCTGATGA ACATTACAGA 
CAAAGGGTCC CCGGGATCTT ACTCAGGAGG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030099089.1
CDS585..1457
Translation

Target ORF information:

RefSeq Version XM_030243229.1
Organism Mus musculus(house mouse)
Definition Mus musculus growth arrest specific 8 (Gas8), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030243229.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGACTTTGA AAGAAATTGA GGCCAAGTAT GATAAGAAGA TGAAGATGCT TAGAGACGAA 
CTTGACCTGC GGAGGAAGAC TGAGATCCAT GAGGTAGAGG AGAGGAAAAA CGGCCAGATC 
AGCACTCTGA TGCAGCGCCA CGAGGAGGCC TTCACGGACA TCAAGAACTA CTACAACGAC 
ATCACTCTCA ACAACCTGGC CCTCATCAAC TCCCTCAAGG AGCAGATGGA GGACATGCGT 
AAGAAGGAGG AACACATGGA GAGGGAGATG GCGGAGGTGA CTCTGCAGAA CCGGCGGCTG 
GCCGACCCGC TGCAGAAAGC CAAGGACGAG ATGAACGAGA TGCAGAAGAG ACTGGGGAAC 
CACGAGAGGG ACAAGCAGAT TCTGGTCTGT ACAAAGGCAC GTTTGAAGGT GGCTGAGAGG 
GAGCTAAAGG ACCTGAAATG GGAACATGAG GTCCTGGAGC AGAGGTTCAT CAAGGTGCAG 
CAGGAGAGGG AAGAGCTCTA TCGGAAGTTT GCAGACGCCA TCCAGGAGGT GCAGCAGAAG 
ACGGGCTTCA AGAACCTGCT GCTGGAGCGG AAGCTGCAGG CCCTGAACGC TGCTGTGGAG 
AAGAGAGAGG TTCAGTTCAA TGAGGTGCTG GCCGCCTCCA ACCTGGACCC CACAGCATTA 
ACGCTTGTGT CCCGCAAACT TGAGGATGTT CTAGAATCGA AGAATACCAC CATCAAGGAC 
TTACAGTATG AGCTGGCCCG GGTCTGCAAG GTGAGGCTAA TCCCCCCATC CGCAGGGCTC 
CACCTTCTCC TCCCTCAGAG CTGTGCCCTG TGCCCAGCTT CACCTGATGA ACATTACAGA 
CAAAGGGTCC CCGGGATCTT ACTCAGGAGG TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A mutation in
Development (Cambridge, England)145(1)(2018 Jan)
Abdelhamed Z,Vuong SM,Hill L,Shula C,Timms A,Beier D,Campbell K,Mangano FT,Stottmann RW,Goto J


book

Mutation of Growth Arrest Specific 8 Reveals a Role in Motile Cilia Function and Human Disease.
PLoS genetics12(7)e1006220(2016 07)
Lewis WR,Malarkey EB,Tritschler D,Bower R,Pasek RC,Porath JD,Birket SE,Saunier S,Antignac C,Knowles MR,Leigh MW,Zariwala MA,Challa AK,Kesterson RA,Rowe SM,Drummond IA,Parant JM,Hildebrandt F,Porter ME,Yoder BK,Berbari NF


book

Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling.
The Journal of biological chemistry286(31)27676-86(2011 Aug)
Evron T,Philipp M,Lu J,Meloni AR,Burkhalter M,Chen W,Caron MG


book

Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells.
Archives of biochemistry and biophysics474(1)136-42(2008 Jun)
Nishimura N,Araki K,Shinahara W,Nakano Y,Nishimura K,Higashio H,Sasaki T


book

Direct interaction of Gas11 with microtubules: implications for the dynein regulatory complex.
Cell motility and the cytoskeleton64(6)461-73(2007 Jun)
Bekker JM,Colantonio JR,Stephens AD,Clarke WT,King SJ,Hill KL,Crosbie RH