E2f8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	E2f8
Entrez Gene ID	108961
Full Name	E2F transcription factor 8
Synonyms	4432406C08Rik,AA410048
General protein information	Preferred Names E2F transcription factor 8 Names transcription factor E2F8 E2F-8 E2f family member 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 B4

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540553.3	XP_006540616.1	transcription factor E2F8 isoform X1
NM_001360812.1	NP_001347741.1	transcription factor E2F8
NM_001013368.5	NP_001013386.2	transcription factor E2F8
XM_006540554.3	XP_006540617.1	transcription factor E2F8 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	E2F8	NP_001243300.1
	E2F8	XP_001095088.1
Bos taurus (cattle)	E2F8	NP_001178163.1
Pan troglodytes (chimpanzee)	E2F8	XP_508325.2
Gallus gallus (chicken)	E2F8	XP_420910.4
Mus musculus (house mouse)	E2f8	NP_001013386.2
Canis lupus familiaris (dog)	E2F8	XP_534087.2
Rattus norvegicus (Norway rat)	E2f8	XP_006229325.1
Danio rerio (zebrafish)	e2f8	XP_005168886.1
Xenopus tropicalis (tropical clawed frog)	e2f8	XP_002942881.1

Gene Ontology
Bibliography

Related articles in PubMed

Atypical E2f functions are critical for pancreas polyploidization.
Matondo RB, Moreno E, Toussaint MJM, Tooten PCJ, van Essen SC, van Liere EA, Youssef SA, Bongiovanni L, de Bruin A
PloS one13(1)e0190899(2018)

E2f8 mediates tumor suppression in postnatal liver development.
Kent LN, Rakijas JB, Pandit SK, Westendorp B, Chen HZ, Huntington JT, Tang X, Bae S, Srivastava A, Senapati S, Koivisto C, Martin CK, Cuitino MC, Perez M, Clouse JM, Chokshi V, Shinde N, Kladney R, Sun D, Perez-Castro A, Matondo RB, Nantasanti S, Mokry M, Huang K, Machiraju R, Fernandez S, Rosol TJ, Coppola V, Pohar KS, Pipas JM, Schmidt CR, de Bruin A, Leone G
The Journal of clinical investigation126(8)2955-69(2016 Aug)

Rb is critical in a mammalian tissue stem cell population.
Wenzel PL, Wu L, de Bruin A, Chong JL, Chen WY, Dureska G, Sites E, Pan T, Sharma A, Huang K, Ridgway R, Mosaliganti K, Sharp R, Machiraju R, Saltz J, Yamamoto H, Cross JC, Robinson ML, Leone G
Genes & development21(1)85-97(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E2F8-mediated transcriptional repression is a critical tumor suppressor mechanism during postnatal liver development
Title: E2f8 mediates tumor suppression in postnatal liver development.

transcription factor E2F8 is involved in the polyploidization during mouse and human decidualization
Title: Involvement of atypical transcription factor E2F8 in the polyploidization during mouse and human decidualization.

Rb and E2F8 collaborate to promote DNA replication.
Title: Inactivation of Rb and E2f8 synergizes to trigger stressed DNA replication during erythroid terminal differentiation.

The results identify E2F8 as a repressor and E2F1 as an activator of a transcriptional network controlling polyploidization in mammalian cells.
Title: E2F8 is essential for polyploidization in mammalian cells.

The specificity of E2F8 in mediating Rb function in erythropoiesis suggests a critical and overlapping roles of Rb and E2f8 in maintaining normal erythropoiesis and in preventing hemolysis.
Title: Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

The following E2f8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E2f8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15440	NM_001360812.1 Latest version!	Mus musculus E2F transcription factor 8 (E2f8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15440	NM_001013368.5 Latest version!	Mus musculus E2F transcription factor 8 (E2f8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15440	XM_006540553.3 Latest version!	Mus musculus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15440	XM_006540554.3 Latest version!	Mus musculus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E2f8 ( NM_001360812.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347741.1 Mus musculus E2F transcription factor 8 (E2f8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15440
Clone ID Related Accession (Same CDS sequence)	XM_006540553.3 , NM_001360812.1 , NM_001013368.5 , XM_006540554.3
Accession Version	NM_001360812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	transcription factor E2F8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC124977.8, BC086675.1 and BX528987.1. On Feb 15, 2018 this sequence version replaced XM_006540553.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY957576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347741.1
CDS	299..2881
Translation	MENQKENLFSEPHKRGLMKSPLHPSSKANMVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSALPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEYEQEFDFIKSCGIEDHVIKSHTGQNGHSDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKYKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPIMPLPASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSNKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKKVKVNLARSGHYKPLAPLDPTVNTELELLTPSLIQPLGVVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPPGLSPTVCPTQPSNATGSKDPTDAPAEKTATDAATTGSLQPAPERHGAKHRSKETTGDRGTKRMITAEDSGPSSVKKPKEDLKALENVPTPTPLFPSGYLIPLTQCSSLGPDSVLSNTENSGTPSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALNSGHPAPAQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPVSPRVEADNLSSRQRRATNHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAENFFRTPGGPTKPTSSPYTDFDGANKTSFGTLFVPQRKLEVSTEDIH

Target ORF information:

RefSeq Version	NM_001360812.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus E2F transcription factor 8 (E2f8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360812.1

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f8 ( NM_001013368.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001013386.2 Mus musculus E2F transcription factor 8 (E2f8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu15440
Clone ID Related Accession (Same CDS sequence)	XM_006540553.3 , NM_001360812.1 , NM_001013368.5 , XM_006540554.3
Accession Version	NM_001013368.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	transcription factor E2F8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK084513.1, AK160240.1 and BX528987.1. On Jul 30, 2009 this sequence version replaced NM_001013368.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK157235.1, AK160240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013386.2
CDS	491..3073
Translation	MENQKENLFSEPHKRGLMKSPLHPSSKANMVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSALPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEYEQEFDFIKSCGIEDHVIKSHTGQNGHSDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKYKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPIMPLPASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSNKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKKVKVNLARSGHYKPLAPLDPTVNTELELLTPSLIQPLGVVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPPGLSPTVCPTQPSNATGSKDPTDAPAEKTATDAATTGSLQPAPERHGAKHRSKETTGDRGTKRMITAEDSGPSSVKKPKEDLKALENVPTPTPLFPSGYLIPLTQCSSLGPDSVLSNTENSGTPSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALNSGHPAPAQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPVSPRVEADNLSSRQRRATNHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAENFFRTPGGPTKPTSSPYTDFDGANKTSFGTLFVPQRKLEVSTEDIH

Target ORF information:

RefSeq Version	NM_001013368.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus E2F transcription factor 8 (E2f8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013368.5

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f8 ( XM_006540553.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540616.1 Mus musculus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15440
Clone ID Related Accession (Same CDS sequence)	XM_006540553.3 , NM_001360812.1 , NM_001013368.5 , XM_006540554.3
Accession Version	XM_006540553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription factor E2F8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540616.1
CDS	299..2881
Translation	MENQKENLFSEPHKRGLMKSPLHPSSKANMVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSALPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEYEQEFDFIKSCGIEDHVIKSHTGQNGHSDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKYKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPIMPLPASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSNKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKKVKVNLARSGHYKPLAPLDPTVNTELELLTPSLIQPLGVVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPPGLSPTVCPTQPSNATGSKDPTDAPAEKTATDAATTGSLQPAPERHGAKHRSKETTGDRGTKRMITAEDSGPSSVKKPKEDLKALENVPTPTPLFPSGYLIPLTQCSSLGPDSVLSNTENSGTPSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALNSGHPAPAQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPVSPRVEADNLSSRQRRATNHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAENFFRTPGGPTKPTSSPYTDFDGANKTSFGTLFVPQRKLEVSTEDIH

Target ORF information:

RefSeq Version	XM_006540553.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540553.3

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E2f8 ( XM_006540554.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540617.1 Mus musculus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15440
Clone ID Related Accession (Same CDS sequence)	XM_006540553.3 , NM_001360812.1 , NM_001013368.5 , XM_006540554.3
Accession Version	XM_006540554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription factor E2F8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540617.1
CDS	194..2776
Translation	MENQKENLFSEPHKRGLMKSPLHPSSKANMVLAEIQPDLGPLTTPTKPKEVSQGEPWTPTANLKMLISAVSPEIRSRDQKRGLSDNRSALPEARDCLHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPKYPNPAVNNDICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLTKTLGTLKSVGEENKYAEQIMMIKRKEYEQEFDFIKSCGIEDHVIKSHTGQNGHSDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVSTPQIVSLEIAAKILIGEDHVEDLDKSKYKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEISPNNSGSSPIMPLPASLEAEQSAKENCAKNLFSTRGKPSFTRHPSLIKLVKSIENDRRKISSAPSSPVKSNKAESSQNSPPVPNKMAQLAAICKMQLEEQSSEPRKKVKVNLARSGHYKPLAPLDPTVNTELELLTPSLIQPLGVVPLIPSPLSSAVPVILPQAPSGPSYAIYLQPAQAQMLTPPPGLSPTVCPTQPSNATGSKDPTDAPAEKTATDAATTGSLQPAPERHGAKHRSKETTGDRGTKRMITAEDSGPSSVKKPKEDLKALENVPTPTPLFPSGYLIPLTQCSSLGPDSVLSNTENSGTPSPNHRIYGSPIAGVIPVASSELTAVNFPPFHVTPLKLMVSPTSMAAVPVGNSPALNSGHPAPAQNPSSAIVNFTLQHLGLISPGVQMSASPGPGAGTVPVSPRVEADNLSSRQRRATNHDSPVLGQSQLNGQPVAGTGAQQPVPVTPKGSQLVAENFFRTPGGPTKPTSSPYTDFDGANKTSFGTLFVPQRKLEVSTEDIH

Target ORF information:

RefSeq Version	XM_006540554.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus E2F transcription factor 8 (E2f8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540554.3

ORF Insert Sequence:

ATGGAGAACC AAAAGGAAAA CCTCTTTTCT GAGCCACATA AAAGGGGACT GATGAAAAGC 
CCCCTGCATC CGTCCTCGAA GGCCAACATG GTGTTGGCTG AGATCCAGCC TGACTTGGGC 
CCTCTAACCA CACCAACCAA GCCCAAGGAA GTCTCCCAAG GAGAGCCATG GACACCCACA 
GCCAACCTGA AAATGCTCAT CAGCGCCGTG AGCCCCGAGA TCCGAAGTCG AGATCAGAAA 
AGGGGCCTGT CTGACAACCG AAGTGCATTA CCTGAAGCTA GAGACTGTTT GCACGAACAC 
TTATCAGGAG ACGAATTTGA GAAATCCCAG CCGAGTCGGA AGGAGAAGAG CTTGGGGTTG 
CTATGCCACA AATTCCTAGC GCGGTACCCC AAGTACCCCA ACCCTGCTGT GAATAACGAC 
ATCTGCCTGG ACGAGGTGGC CGAGGAGCTC AATGTTGAAC GTCGGCGGAT TTATGACATT 
GTGAATGTCC TAGAGAGCCT GCACATGGTG AGCCGCCTTG CCAAAAACAG GTACACTTGG 
CATGGCCGGC ACAACCTCAC CAAAACCCTC GGGACGCTGA AGAGTGTCGG GGAAGAGAAC 
AAGTACGCTG AGCAGATCAT GATGATCAAA AGGAAAGAAT ATGAGCAAGA GTTTGATTTT 
ATCAAGAGCT GTGGCATAGA GGACCACGTG ATCAAGTCAC ACACTGGCCA GAATGGGCAT 
TCAGACATGT GCTTCGTAGA ACTCCCTGGA GTAGAATTCC GGGCAGCTTC TGTAAACAGC 
CGCAAAGACA AGTCCTTACG CGTGATGAGC CAGAAGTTTG TGATGCTGTT CTTGGTGTCG 
ACGCCTCAGA TAGTGAGCCT GGAAATTGCT GCCAAGATTT TAATTGGGGA AGACCACGTG 
GAAGATCTGG ATAAAAGCAA GTATAAAACA AAAATTAGGA GGCTGTATGA CATTGCTAAT 
GTCCTGAGTA GCTTGGATCT TATCAAGAAA GTCCATGTTA CAGAAGAAAG AGGTCGAAAA 
CCAGCTTTTA AATGGACGGG CCCAGAAATC AGCCCAAACA ACAGTGGTTC CAGCCCCATC 
ATGCCTCTTC CTGCCTCCTT AGAGGCTGAG CAGTCTGCAA AAGAGAACTG TGCCAAAAAC 
CTCTTCTCTA CGCGGGGGAA ACCCAGCTTC ACTCGACACC CGTCCCTCAT CAAGTTGGTA 
AAGAGCATAG AAAATGATCG GAGGAAGATC AGTTCCGCTC CCAGCAGCCC TGTCAAGAGC 
AACAAAGCTG AGAGTTCTCA AAATTCTCCA CCCGTCCCAA ACAAAATGGC TCAGCTCGCC 
GCTATTTGCA AGATGCAGTT GGAAGAGCAG TCAAGTGAAC CCAGGAAGAA AGTGAAAGTA 
AACCTAGCAA GATCTGGGCA CTACAAACCA CTGGCTCCCC TGGACCCTAC AGTGAACACT 
GAGCTAGAAC TGCTTACACC GTCCCTTATC CAGCCCCTGG GCGTGGTCCC CCTGATCCCT 
AGCCCATTGT CATCTGCAGT GCCTGTGATC CTACCTCAGG CCCCTTCGGG CCCATCCTAT 
GCCATCTACT TACAACCTGC CCAAGCCCAA ATGTTGACAC CACCCCCTGG CCTGAGCCCA 
ACCGTCTGCC CCACCCAGCC CTCTAATGCT ACTGGATCCA AAGACCCTAC AGACGCCCCT 
GCTGAGAAGA CGGCCACAGA CGCCGCCACA ACCGGAAGCT TGCAGCCAGC ACCAGAAAGG 
CATGGTGCTA AGCATCGAAG CAAGGAGACG ACCGGAGATC GGGGTACAAA GAGGATGATC 
ACCGCGGAGG ACAGTGGTCC CAGTTCTGTA AAGAAACCTA AAGAGGACCT GAAAGCGCTT 
GAGAACGTCC CCACCCCCAC GCCCCTGTTC CCATCAGGAT ACCTAATCCC TCTTACCCAG 
TGCTCATCCC TAGGGCCAGA CTCTGTGTTG TCTAACACTG AAAACTCAGG TACACCCTCT 
CCAAACCACC GGATCTATGG CTCCCCCATT GCAGGTGTGA TCCCAGTGGC ATCATCGGAA 
CTCACTGCTG TGAATTTTCC CCCATTCCAT GTGACACCTC TGAAACTTAT GGTCTCCCCA 
ACATCTATGG CGGCCGTACC TGTTGGGAAC AGCCCAGCCC TCAACTCCGG CCACCCTGCT 
CCCGCCCAGA ACCCAAGCTC GGCTATCGTA AACTTTACCC TGCAGCACTT GGGACTCATC 
TCCCCGGGCG TGCAGATGTC TGCCAGCCCG GGGCCTGGAG CTGGCACAGT CCCGGTATCC 
CCACGTGTAG AAGCAGATAA CTTGAGCTCT CGGCAAAGGA GGGCCACCAA CCATGACTCG 
CCAGTCCTGG GCCAGAGCCA ACTAAACGGA CAACCAGTCG CGGGGACAGG GGCACAGCAG 
CCTGTTCCTG TGACACCCAA AGGCTCCCAG TTGGTGGCTG AAAATTTCTT CCGTACTCCG 
GGTGGACCAA CTAAGCCTAC CAGCTCACCC TACACAGATT TCGATGGTGC TAACAAAACC 
TCCTTCGGAA CCCTCTTTGT CCCACAGCGC AAACTGGAAG TCTCAACTGA GGATATCCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Atypical E2f functions are critical for pancreas polyploidization.
PloS one13(1)e0190899(2018)
Matondo RB,Moreno E,Toussaint MJM,Tooten PCJ,van Essen SC,van Liere EA,Youssef SA,Bongiovanni L,de Bruin A

E2f8 mediates tumor suppression in postnatal liver development.
The Journal of clinical investigation126(8)2955-69(2016 Aug)
Kent LN,Rakijas JB,Pandit SK,Westendorp B,Chen HZ,Huntington JT,Tang X,Bae S,Srivastava A,Senapati S,Koivisto C,Martin CK,Cuitino MC,Perez M,Clouse JM,Chokshi V,Shinde N,Kladney R,Sun D,Perez-Castro A,Matondo RB,Nantasanti S,Mokry M,Huang K,Machiraju R,Fernandez S,Rosol TJ,Coppola V,Pohar KS,Pipas JM,Schmidt CR,de Bruin A,Leone G

Rb is critical in a mammalian tissue stem cell population.
Genes & development21(1)85-97(2007 Jan)
Wenzel PL,Wu L,de Bruin A,Chong JL,Chen WY,Dureska G,Sites E,Pan T,Sharma A,Huang K,Ridgway R,Mosaliganti K,Sharp R,Machiraju R,Saltz J,Yamamoto H,Cross JC,Robinson ML,Leone G

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E2f8 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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