Gene Symbol | Npnt |
Entrez Gene ID | 114249 |
Full Name | nephronectin |
Synonyms | 1110009H02Rik,AA682063,AI314031,Nctn,POEM |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Npnt cDNA ORF clone
gRNAs
Gene Symbol | Npnt |
Entrez Gene ID | 114249 |
Full Name | nephronectin |
Synonyms | 1110009H02Rik,AA682063,AI314031,Nctn,POEM |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
NM_001287103.1 | NP_001274032.1 | nephronectin isoform e |
NM_001287102.1 | NP_001274031.1 | nephronectin isoform d precursor |
NM_001029836.2 | NP_001025007.1 | nephronectin isoform b precursor |
XM_006500915.3 | XP_006500978.1 | nephronectin isoform X1 |
XM_006500915.4 | XP_006500978.1 | nephronectin isoform X1 |
NM_033525.3 | NP_277060.2 | nephronectin isoform a precursor |
NM_001287101.1 | NP_001274030.1 | nephronectin isoform c precursor |
Gallus gallus (chicken) | NPNT | XP_420498.3 |
Pan troglodytes (chimpanzee) | NPNT | XP_517382.3 |
NPNT | XP_001084904.2 | |
Bos taurus (cattle) | NPNT | XP_005207735.1 |
Homo sapiens (human) | NPNT | NP_001171619.1 |
Canis lupus familiaris (dog) | NPNT | XP_005639300.1 |
Danio rerio (zebrafish) | npnt | NP_001139052.1 |
Xenopus tropicalis (tropical clawed frog) | npnt | XP_004911145.1 |
Mus musculus (house mouse) | Npnt | NP_001274030.1 |
Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli.
Zimmerman SE, Hiremath C, Tsunezumi J, Yang Z, Finney B, Marciano DK
Journal of the American Society of Nephrology : JASN(2018 Jan)
Nephronectin is Decreased in Metastatic Breast Carcinoma and Related to Metastatic Organs.
Dilmac S, Erin N, Demir N, Tanriover G
Pathology oncology research : POR(2017 Aug)
Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains.
Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S
Scientific reports745181(2017 Mar)
The ECM protein nephronectin promotes kidney development via integrin alpha8beta1-mediated stimulation of Gdnf expression.
Linton JM, Martin GR, Reichardt LF
Development (Cambridge, England)134(13)2501-9(2007 Jul)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
These results suggest novel mechanisms for Wnt3a-induced osteoblast proliferation and cell survival via Npnt gene expression
Title: Wnt/?-catenin signaling activates nephronectin expression in osteoblasts.
we found that bone morphogenic protein-2 (BMP-2), known as an osteogenesis inducing cytokine, strongly up-regulated the expression of Npnt mRNA in a murine skeletal muscle cell line (C2C12) via the BMP-SMAD signaling pathway
Title: Nephronectin Expression Is Up-Regulated by BMP-2.
Nephronectin is capable of binding to heparan sulfate proteoglycans in basement membranes via the MAM domain.
Title: Nephronectin binds to heparan sulfate proteoglycans via its MAM domain.
These results indicate that Npnt plays an important role in the initiation of hepatitis by recruiting CD4(+) T cells or NKT cells into the foci of inflammation.
Title: Nephronectin is upregulated in acute and chronic hepatitis and aggravates liver injury by recruiting CD4 positive cells.
canonical TGF-b signaling through Smad2 regulates Npnt geneexpression in osteoblasts.
Title: Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells.
The following Npnt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npnt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu08941 | |
Clone ID Related Accession (Same CDS sequence) | NM_033525.3 | |
Accession Version | NM_033525.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP |
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Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-24 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform a precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139942.4. On Nov 28, 2013 this sequence version replaced NM_033525.2. Transcript Variant: This variant (1) lacks an in-frame exon in the 5' coding region compared to variant 3. The encoded isoform (a) is shorter than isoform c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY035899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_277060.2 |
CDS | 249..1985 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu12278 | |
Clone ID Related Accession (Same CDS sequence) | NM_001029836.2 | |
Accession Version | NM_001029836.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-27 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform b precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB059656.1, AK160311.1 and AC139942.4. On Nov 28, 2013 this sequence version replaced NM_001029836.1. Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region compared to variant 3. The encoded isoform (b) is shorter than isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB059656.1, BC068308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001025007.1 |
CDS | 249..1934 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu17048 | |
Clone ID Related Accession (Same CDS sequence) | NM_001287103.1 | |
Accession Version | NM_001287103.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-23 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform e | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158764.1, AK160311.1 and AC139942.4. Transcript Variant: This variant (5) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region compared to variant 3. The resulting isoform (e) has a shorter N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGAACACTT TCGGCAGCTA CAAGTGCTAC TGTCTCAACG GATACATGCT GCTGCCAGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001274032.1 |
CDS | 279..1652 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGAACACTT TCGGCAGCTA CAAGTGCTAC TGTCTCAACG GATACATGCT GCTGCCAGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu17342 | |
Clone ID Related Accession (Same CDS sequence) | NM_001287102.1 | |
Accession Version | NM_001287102.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-23 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform d precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB059656.1, AF397007.1, AK160311.1 and AC139942.4. Transcript Variant: This variant (4) lacks an in-frame exon in the 5' coding region compared to variant 3. The encoded isoform (d) is shorter than isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF397007.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001274031.1 |
CDS | 249..2027 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu17344 | |
Clone ID Related Accession (Same CDS sequence) | NM_001287101.1 | |
Accession Version | NM_001287101.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-09-24 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform c precursor | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB059656.1, AF397008.1, AK160311.1 and AC139942.4. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF397008.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001274030.1 |
CDS | 249..2078 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31567 | |
Clone ID Related Accession (Same CDS sequence) | XM_006500915.3 , XM_006500915.4 | |
Accession Version | XM_006500915.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006500915.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006500978.1 |
CDS | 234..1826 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31567 | |
Clone ID Related Accession (Same CDS sequence) | XM_006500915.3 , XM_006500915.4 | |
Accession Version | XM_006500915.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | nephronectin isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006500915.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006500978.1 |
CDS | 232..1824 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCTGTGC TCCTAGCGGC GGTGCTCGCG TCCTCGCTCT ACCTGCAGGT GGCTGCCGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Nephronectin Regulates Mesangial Cell Adhesion and Behavior in Glomeruli. |
Nephronectin is Decreased in Metastatic Breast Carcinoma and Related to Metastatic Organs. |
Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains. |
The ECM protein nephronectin promotes kidney development via integrin alpha8beta1-mediated stimulation of Gdnf expression. |