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Necab2 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Necab2
Entrez Gene ID 117148
Full Name N-terminal EF-hand calcium binding protein 2
Synonyms Efcbp2
General protein information
Preferred Names

N-terminal EF-hand calcium binding protein 2

Names

N-terminal EF-hand calcium-binding protein 2
EF hand calcium binding protein 2
neuronal calcium binding 2
neuronal calcium-binding protein 2
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

8

8|8 E1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006530583.1 XP_006530646.1 N-terminal EF-hand calcium-binding protein 2 isoform X1
XM_017312526.2 XP_017168015.1 N-terminal EF-hand calcium-binding protein 2 isoform X2
NM_054095.2 NP_473436.1 N-terminal EF-hand calcium-binding protein 2
XM_017312526.1 XP_017168015.1 N-terminal EF-hand calcium-binding protein 2 isoform X2
XM_030243249.1 XP_030099109.1 N-terminal EF-hand calcium-binding protein 2 isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) NECAB2 NP_061938.2
Bos taurus (cattle) NECAB2 NP_001092447.1
Danio rerio (zebrafish) necab2 NP_001025366.1
Gallus gallus (chicken) NECAB2 XP_414072.1
Xenopus tropicalis (tropical clawed frog) LOC101731915 XP_004913648.1
Pan troglodytes (chimpanzee) NECAB2 XP_001150555.1
LOC718169 XP_001110489.2
Mus musculus (house mouse) Necab2 NP_473436.1
Rattus norvegicus (Norway rat) Necab2 NP_596906.1
Canis lupus familiaris (dog) NECAB2 XP_005620942.1

Gene Ontology


Bibliography

Related articles in PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Histopathology reveals correlative and unique phenotypes in a high-throughput mouse phenotyping screen.
Adissu HA, Estabel J, Sunter D, Tuck E, Hooks Y, Carragher DM, Clarke K, Karp NA, , Newbigging S, Jones N, Morikawa L, White JK, McKerlie C
Disease models & mechanisms7(5)515-24(2014 May)

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Miller JA, Nathanson J, Franjic D, Shim S, Dalley RA, Shapouri S, Smith KA, Sunkin SM, Bernard A, Bennett JL, Lee CK, Hawrylycz MJ, Jones AR, Amaral DG, ?estan N, Gage FH, Lein ES
Development (Cambridge, England)140(22)4633-44(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The genes for the three Necab's, including Necab-2, were expressed in specific, non-overlapping areas of the hippocampus.
Title: Expression of the calcium binding proteins Necab-1,-2 and -3 in the adult mouse hippocampus and dentate gyrus.

The following Necab2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Necab2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu41160 XM_006530583.1
Latest version!		 Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu41160 XM_030243249.1
Latest version!		 Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OMu82327 XM_017312526.2
Latest version!		 Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OMu16341 NM_054095.2
Latest version!		 Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
View Old Accession Versions >>
OMu82327 XM_017312526.1 Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu41160
Clone ID Related Accession (Same CDS sequence) XM_006530583.1 , XM_030243249.1
Accession Version XM_006530583.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAGAGCC ACGTGGACAC CAAGGAACTG TGTGACTACT TTGTGGAGCA CATGGGAGAC 
TATGAAGATG TCCTGGCCTC CCTGGAGACT TTGAACCACT CAGTCCTGAA GGCCATGGGC 
TACACTAAGA AGGTGTATGA AGGTGGGAGC AATGTGGACC AGTTTGTGAC ACGCTTCCTT 
CTGAAGGAGA CAGCCAATCA GATCCAGTCG CTGCTCAGCT CTGTGGAAAG TGCTGTAGAA 
GCCATCGAAG AACAGACCAG CCAGATCCGA CAGGACCACT GCAAACCCAG CCATGCCGTC 
AACGAGAGCC GCTACGGAGG CCCTACTCCT CCCTACATCC CCAACCACAA GCTTGTGGCC 
CCGGAGCCGA TGAAGAGCCT GCCTGTGGCC ACAGGGGAGC CAAAGGAGGA TGGGCTGGAG 
GGCCAGATAA GCCGGTTGGC AGAGCTGATT GGGAGACTGG AGAGCAAGAC CCTCTCGTTT 
GACCTGCAAC AGCGCCTCTC GGATGAAGAA GGCACCAACA TGCACCTGCA GCTGGTCCGG 
CAGGAAATGG CCGTGTGCCC TGAGCAGCTG AGTGAGTTCT TGGATTCCCT GAGACAGTAC 
CTCCGGAGCA CGGCTGAAGA AAGGAACTGC TTCCATGTTG CCGCAGTGAG GATGGCAGAT 
GGCCTAACCT TCGTCATCTA CGAGTTCTGG GAGACAGAGG AGGAGTGGAA GAGGCACCTG 
CAGAGCCCTG TGTGCAAGGC CTTCCGACAC GTCAAGGTTG ACACGCTGAG CCAGCCTGAA 
GCACTCTCCC AGATCTCCGT GCCAGCTGCC TGGTGCACCT CTGGCCGAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006530646.1
CDS80..913
Translation

Target ORF information:

RefSeq Version XM_006530583.1
Organism Mus musculus(house mouse)
Definition Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006530583.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAGAGCC ACGTGGACAC CAAGGAACTG TGTGACTACT TTGTGGAGCA CATGGGAGAC 
TATGAAGATG TCCTGGCCTC CCTGGAGACT TTGAACCACT CAGTCCTGAA GGCCATGGGC 
TACACTAAGA AGGTGTATGA AGGTGGGAGC AATGTGGACC AGTTTGTGAC ACGCTTCCTT 
CTGAAGGAGA CAGCCAATCA GATCCAGTCG CTGCTCAGCT CTGTGGAAAG TGCTGTAGAA 
GCCATCGAAG AACAGACCAG CCAGATCCGA CAGGACCACT GCAAACCCAG CCATGCCGTC 
AACGAGAGCC GCTACGGAGG CCCTACTCCT CCCTACATCC CCAACCACAA GCTTGTGGCC 
CCGGAGCCGA TGAAGAGCCT GCCTGTGGCC ACAGGGGAGC CAAAGGAGGA TGGGCTGGAG 
GGCCAGATAA GCCGGTTGGC AGAGCTGATT GGGAGACTGG AGAGCAAGAC CCTCTCGTTT 
GACCTGCAAC AGCGCCTCTC GGATGAAGAA GGCACCAACA TGCACCTGCA GCTGGTCCGG 
CAGGAAATGG CCGTGTGCCC TGAGCAGCTG AGTGAGTTCT TGGATTCCCT GAGACAGTAC 
CTCCGGAGCA CGGCTGAAGA AAGGAACTGC TTCCATGTTG CCGCAGTGAG GATGGCAGAT 
GGCCTAACCT TCGTCATCTA CGAGTTCTGG GAGACAGAGG AGGAGTGGAA GAGGCACCTG 
CAGAGCCCTG TGTGCAAGGC CTTCCGACAC GTCAAGGTTG ACACGCTGAG CCAGCCTGAA 
GCACTCTCCC AGATCTCCGT GCCAGCTGCC TGGTGCACCT CTGGCCGAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu82327
Clone ID Related Accession (Same CDS sequence) XM_017312526.2 , XM_017312526.1
Accession Version XM_017312526.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 783bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGAGACT ATGAAGATGT CCTGGCCTCC CTGGAGACTT TGAACCACTC AGTCCTGAAG 
GCCATGGGCT ACACTAAGAA GGTGTATGAA GGTGGGAGCA ATGTGGACCA GTTTGTGACA 
CGCTTCCTTC TGAAGGAGAC AGCCAATCAG ATCCAGTCGC TGCTCAGCTC TGTGGAAAGT 
GCTGTAGAAG CCATCGAAGA ACAGACCAGC CAGATCCGAC AGGACCACTG CAAACCCAGC 
CATGCCGTCA ACGAGAGCCG CTACGGAGGC CCTACTCCTC CCTACATCCC CAACCACAAG 
CTTGTGGCCC CGGAGCCGAT GAAGAGCCTG CCTGTGGCCA CAGGGGAGCC AAAGGAGGAT 
GGGCTGGAGG GCCAGATAAG CCGGTTGGCA GAGCTGATTG GGAGACTGGA GAGCAAGACC 
CTCTCGTTTG ACCTGCAACA GCGCCTCTCG GATGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAAATGGC CGTGTGCCCT GAGCAGCTGA GTGAGTTCTT GGATTCCCTG 
AGACAGTACC TCCGGAGCAC GGCTGAAGAA AGGAACTGCT TCCATGTTGC CGCAGTGAGG 
ATGGCAGATG GCCTAACCTT CGTCATCTAC GAGTTCTGGG AGACAGAGGA GGAGTGGAAG 
AGGCACCTGC AGAGCCCTGT GTGCAAGGCC TTCCGACACG TCAAGGTTGA CACGCTGAGC 
CAGCCTGAAG CACTCTCCCA GATCTCCGTG CCAGCTGCCT GGTGCACCTC TGGCCGAGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017168015.1
CDS3193..3975
Translation

Target ORF information:

RefSeq Version XM_017312526.1
Organism Mus musculus(house mouse)
Definition Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017312526.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGAGACT ATGAAGATGT CCTGGCCTCC CTGGAGACTT TGAACCACTC AGTCCTGAAG 
GCCATGGGCT ACACTAAGAA GGTGTATGAA GGTGGGAGCA ATGTGGACCA GTTTGTGACA 
CGCTTCCTTC TGAAGGAGAC AGCCAATCAG ATCCAGTCGC TGCTCAGCTC TGTGGAAAGT 
GCTGTAGAAG CCATCGAAGA ACAGACCAGC CAGATCCGAC AGGACCACTG CAAACCCAGC 
CATGCCGTCA ACGAGAGCCG CTACGGAGGC CCTACTCCTC CCTACATCCC CAACCACAAG 
CTTGTGGCCC CGGAGCCGAT GAAGAGCCTG CCTGTGGCCA CAGGGGAGCC AAAGGAGGAT 
GGGCTGGAGG GCCAGATAAG CCGGTTGGCA GAGCTGATTG GGAGACTGGA GAGCAAGACC 
CTCTCGTTTG ACCTGCAACA GCGCCTCTCG GATGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAAATGGC CGTGTGCCCT GAGCAGCTGA GTGAGTTCTT GGATTCCCTG 
AGACAGTACC TCCGGAGCAC GGCTGAAGAA AGGAACTGCT TCCATGTTGC CGCAGTGAGG 
ATGGCAGATG GCCTAACCTT CGTCATCTAC GAGTTCTGGG AGACAGAGGA GGAGTGGAAG 
AGGCACCTGC AGAGCCCTGT GTGCAAGGCC TTCCGACACG TCAAGGTTGA CACGCTGAGC 
CAGCCTGAAG CACTCTCCCA GATCTCCGTG CCAGCTGCCT GGTGCACCTC TGGCCGAGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu41160
Clone ID Related Accession (Same CDS sequence) XM_006530583.1 , XM_030243249.1
Accession Version XM_030243249.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 834bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAGAGCC ACGTGGACAC CAAGGAACTG TGTGACTACT TTGTGGAGCA CATGGGAGAC 
TATGAAGATG TCCTGGCCTC CCTGGAGACT TTGAACCACT CAGTCCTGAA GGCCATGGGC 
TACACTAAGA AGGTGTATGA AGGTGGGAGC AATGTGGACC AGTTTGTGAC ACGCTTCCTT 
CTGAAGGAGA CAGCCAATCA GATCCAGTCG CTGCTCAGCT CTGTGGAAAG TGCTGTAGAA 
GCCATCGAAG AACAGACCAG CCAGATCCGA CAGGACCACT GCAAACCCAG CCATGCCGTC 
AACGAGAGCC GCTACGGAGG CCCTACTCCT CCCTACATCC CCAACCACAA GCTTGTGGCC 
CCGGAGCCGA TGAAGAGCCT GCCTGTGGCC ACAGGGGAGC CAAAGGAGGA TGGGCTGGAG 
GGCCAGATAA GCCGGTTGGC AGAGCTGATT GGGAGACTGG AGAGCAAGAC CCTCTCGTTT 
GACCTGCAAC AGCGCCTCTC GGATGAAGAA GGCACCAACA TGCACCTGCA GCTGGTCCGG 
CAGGAAATGG CCGTGTGCCC TGAGCAGCTG AGTGAGTTCT TGGATTCCCT GAGACAGTAC 
CTCCGGAGCA CGGCTGAAGA AAGGAACTGC TTCCATGTTG CCGCAGTGAG GATGGCAGAT 
GGCCTAACCT TCGTCATCTA CGAGTTCTGG GAGACAGAGG AGGAGTGGAA GAGGCACCTG 
CAGAGCCCTG TGTGCAAGGC CTTCCGACAC GTCAAGGTTG ACACGCTGAG CCAGCCTGAA 
GCACTCTCCC AGATCTCCGT GCCAGCTGCC TGGTGCACCT CTGGCCGAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030099109.1
CDS373..1206
Translation

Target ORF information:

RefSeq Version XM_030243249.1
Organism Mus musculus(house mouse)
Definition Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030243249.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCAGAGCC ACGTGGACAC CAAGGAACTG TGTGACTACT TTGTGGAGCA CATGGGAGAC 
TATGAAGATG TCCTGGCCTC CCTGGAGACT TTGAACCACT CAGTCCTGAA GGCCATGGGC 
TACACTAAGA AGGTGTATGA AGGTGGGAGC AATGTGGACC AGTTTGTGAC ACGCTTCCTT 
CTGAAGGAGA CAGCCAATCA GATCCAGTCG CTGCTCAGCT CTGTGGAAAG TGCTGTAGAA 
GCCATCGAAG AACAGACCAG CCAGATCCGA CAGGACCACT GCAAACCCAG CCATGCCGTC 
AACGAGAGCC GCTACGGAGG CCCTACTCCT CCCTACATCC CCAACCACAA GCTTGTGGCC 
CCGGAGCCGA TGAAGAGCCT GCCTGTGGCC ACAGGGGAGC CAAAGGAGGA TGGGCTGGAG 
GGCCAGATAA GCCGGTTGGC AGAGCTGATT GGGAGACTGG AGAGCAAGAC CCTCTCGTTT 
GACCTGCAAC AGCGCCTCTC GGATGAAGAA GGCACCAACA TGCACCTGCA GCTGGTCCGG 
CAGGAAATGG CCGTGTGCCC TGAGCAGCTG AGTGAGTTCT TGGATTCCCT GAGACAGTAC 
CTCCGGAGCA CGGCTGAAGA AAGGAACTGC TTCCATGTTG CCGCAGTGAG GATGGCAGAT 
GGCCTAACCT TCGTCATCTA CGAGTTCTGG GAGACAGAGG AGGAGTGGAA GAGGCACCTG 
CAGAGCCCTG TGTGCAAGGC CTTCCGACAC GTCAAGGTTG ACACGCTGAG CCAGCCTGAA 
GCACTCTCCC AGATCTCCGT GCCAGCTGCC TGGTGCACCT CTGGCCGAGA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu82327
Clone ID Related Accession (Same CDS sequence) XM_017312526.2 , XM_017312526.1
Accession Version XM_017312526.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 783bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product N-terminal EF-hand calcium-binding protein 2 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGAGACT ATGAAGATGT CCTGGCCTCC CTGGAGACTT TGAACCACTC AGTCCTGAAG 
GCCATGGGCT ACACTAAGAA GGTGTATGAA GGTGGGAGCA ATGTGGACCA GTTTGTGACA 
CGCTTCCTTC TGAAGGAGAC AGCCAATCAG ATCCAGTCGC TGCTCAGCTC TGTGGAAAGT 
GCTGTAGAAG CCATCGAAGA ACAGACCAGC CAGATCCGAC AGGACCACTG CAAACCCAGC 
CATGCCGTCA ACGAGAGCCG CTACGGAGGC CCTACTCCTC CCTACATCCC CAACCACAAG 
CTTGTGGCCC CGGAGCCGAT GAAGAGCCTG CCTGTGGCCA CAGGGGAGCC AAAGGAGGAT 
GGGCTGGAGG GCCAGATAAG CCGGTTGGCA GAGCTGATTG GGAGACTGGA GAGCAAGACC 
CTCTCGTTTG ACCTGCAACA GCGCCTCTCG GATGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAAATGGC CGTGTGCCCT GAGCAGCTGA GTGAGTTCTT GGATTCCCTG 
AGACAGTACC TCCGGAGCAC GGCTGAAGAA AGGAACTGCT TCCATGTTGC CGCAGTGAGG 
ATGGCAGATG GCCTAACCTT CGTCATCTAC GAGTTCTGGG AGACAGAGGA GGAGTGGAAG 
AGGCACCTGC AGAGCCCTGT GTGCAAGGCC TTCCGACACG TCAAGGTTGA CACGCTGAGC 
CAGCCTGAAG CACTCTCCCA GATCTCCGTG CCAGCTGCCT GGTGCACCTC TGGCCGAGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_017168015.1
CDS3192..3974
Translation

Target ORF information:

RefSeq Version XM_017312526.2
Organism Mus musculus(house mouse)
Definition Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017312526.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGGAGACT ATGAAGATGT CCTGGCCTCC CTGGAGACTT TGAACCACTC AGTCCTGAAG 
GCCATGGGCT ACACTAAGAA GGTGTATGAA GGTGGGAGCA ATGTGGACCA GTTTGTGACA 
CGCTTCCTTC TGAAGGAGAC AGCCAATCAG ATCCAGTCGC TGCTCAGCTC TGTGGAAAGT 
GCTGTAGAAG CCATCGAAGA ACAGACCAGC CAGATCCGAC AGGACCACTG CAAACCCAGC 
CATGCCGTCA ACGAGAGCCG CTACGGAGGC CCTACTCCTC CCTACATCCC CAACCACAAG 
CTTGTGGCCC CGGAGCCGAT GAAGAGCCTG CCTGTGGCCA CAGGGGAGCC AAAGGAGGAT 
GGGCTGGAGG GCCAGATAAG CCGGTTGGCA GAGCTGATTG GGAGACTGGA GAGCAAGACC 
CTCTCGTTTG ACCTGCAACA GCGCCTCTCG GATGAAGAAG GCACCAACAT GCACCTGCAG 
CTGGTCCGGC AGGAAATGGC CGTGTGCCCT GAGCAGCTGA GTGAGTTCTT GGATTCCCTG 
AGACAGTACC TCCGGAGCAC GGCTGAAGAA AGGAACTGCT TCCATGTTGC CGCAGTGAGG 
ATGGCAGATG GCCTAACCTT CGTCATCTAC GAGTTCTGGG AGACAGAGGA GGAGTGGAAG 
AGGCACCTGC AGAGCCCTGT GTGCAAGGCC TTCCGACACG TCAAGGTTGA CACGCTGAGC 
CAGCCTGAAG CACTCTCCCA GATCTCCGTG CCAGCTGCCT GGTGCACCTC TGGCCGAGAC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu16341
Clone ID Related Accession (Same CDS sequence) NM_054095.2
Accession Version NM_054095.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1170bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-09-30
Organism Mus musculus(house mouse)
Product N-terminal EF-hand calcium-binding protein 2
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC166781.7. On Nov 17, 2006 this sequence version replaced NM_054095.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF411253.1, AK082727.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCCGCAGG GCCGCGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGGGAA 
CCCCGTGCAC CGCTCGTCCC CGACATCCCC TCCGCGGACC CCGGCCCCGG GCCCGCCGCA 
TCGCGAGGGG GCACAGCCGT CATCCTGGAC ATTTTCCGCC GCGCCGATAA AAATGATGAT 
GGGAAGCTGT CCCTGGAGGA GTTTCAACTC TTCTTCGCAG ATGGCGTCCT TAATGAGAAG 
GAGCTGGAGG GCCTCTTCCA TACCATCGAT TCAGACAACA CCAACCACGT GGACACCAAG 
GAACTGTGTG ACTACTTTGT GGAGCACATG GGAGACTATG AAGATGTCCT GGCCTCCCTG 
GAGACTTTGA ACCACTCAGT CCTGAAGGCC ATGGGCTACA CTAAGAAGGT GTATGAAGGT 
GGGAGCAATG TGGACCAGTT TGTGACACGC TTCCTTCTGA AGGAGACAGC CAATCAGATC 
CAGTCGCTGC TCAGCTCTGT GGAAAGTGCT GTAGAAGCCA TCGAAGAACA GACCAGCCAG 
ATCCGACAGG ACCACTGCAA ACCCAGCCAT GCCGTCAACG AGAGCCGCTA CGGAGGCCCT 
ACTCCTCCCT ACATCCCCAA CCACAAGCTT GTGGCCCCGG AGCCGATGAA GAGCCTGCCT 
GTGGCCACAG GGGAGCCAAA GGAGGATGGG CTGGAGGGCC AGATAAGCCG GTTGGCAGAG 
CTGATTGGGA GACTGGAGAG CAAGACCCTC TCGTTTGACC TGCAACAGCG CCTCTCGGAT 
GAAGAAGGCA CCAACATGCA CCTGCAGCTG GTCCGGCAGG AAATGGCCGT GTGCCCTGAG 
CAGCTGAGTG AGTTCTTGGA TTCCCTGAGA CAGTACCTCC GGAGCACGGC TGAAGAAAGG 
AACTGCTTCC ATGTTGCCGC AGTGAGGATG GCAGATGGCC TAACCTTCGT CATCTACGAG 
TTCTGGGAGA CAGAGGAGGA GTGGAAGAGG CACCTGCAGA GCCCTGTGTG CAAGGCCTTC 
CGACACGTCA AGGTTGACAC GCTGAGCCAG CCTGAAGCAC TCTCCCAGAT CTCCGTGCCA 
GCTGCCTGGT GCACCTCTGG CCGAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_473436.1
CDS337..1506
Translation

Target ORF information:

RefSeq Version NM_054095.2
Organism Mus musculus(house mouse)
Definition Mus musculus N-terminal EF-hand calcium binding protein 2 (Necab2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_054095.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCCGCAGG GCCGCGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGGGAA 
CCCCGTGCAC CGCTCGTCCC CGACATCCCC TCCGCGGACC CCGGCCCCGG GCCCGCCGCA 
TCGCGAGGGG GCACAGCCGT CATCCTGGAC ATTTTCCGCC GCGCCGATAA AAATGATGAT 
GGGAAGCTGT CCCTGGAGGA GTTTCAACTC TTCTTCGCAG ATGGCGTCCT TAATGAGAAG 
GAGCTGGAGG GCCTCTTCCA TACCATCGAT TCAGACAACA CCAACCACGT GGACACCAAG 
GAACTGTGTG ACTACTTTGT GGAGCACATG GGAGACTATG AAGATGTCCT GGCCTCCCTG 
GAGACTTTGA ACCACTCAGT CCTGAAGGCC ATGGGCTACA CTAAGAAGGT GTATGAAGGT 
GGGAGCAATG TGGACCAGTT TGTGACACGC TTCCTTCTGA AGGAGACAGC CAATCAGATC 
CAGTCGCTGC TCAGCTCTGT GGAAAGTGCT GTAGAAGCCA TCGAAGAACA GACCAGCCAG 
ATCCGACAGG ACCACTGCAA ACCCAGCCAT GCCGTCAACG AGAGCCGCTA CGGAGGCCCT 
ACTCCTCCCT ACATCCCCAA CCACAAGCTT GTGGCCCCGG AGCCGATGAA GAGCCTGCCT 
GTGGCCACAG GGGAGCCAAA GGAGGATGGG CTGGAGGGCC AGATAAGCCG GTTGGCAGAG 
CTGATTGGGA GACTGGAGAG CAAGACCCTC TCGTTTGACC TGCAACAGCG CCTCTCGGAT 
GAAGAAGGCA CCAACATGCA CCTGCAGCTG GTCCGGCAGG AAATGGCCGT GTGCCCTGAG 
CAGCTGAGTG AGTTCTTGGA TTCCCTGAGA CAGTACCTCC GGAGCACGGC TGAAGAAAGG 
AACTGCTTCC ATGTTGCCGC AGTGAGGATG GCAGATGGCC TAACCTTCGT CATCTACGAG 
TTCTGGGAGA CAGAGGAGGA GTGGAAGAGG CACCTGCAGA GCCCTGTGTG CAAGGCCTTC 
CGACACGTCA AGGTTGACAC GCTGAGCCAG CCTGAAGCAC TCTCCCAGAT CTCCGTGCCA 
GCTGCCTGGT GCACCTCTGG CCGAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR


book

Histopathology reveals correlative and unique phenotypes in a high-throughput mouse phenotyping screen.
Disease models & mechanisms7(5)515-24(2014 May)
Adissu HA,Estabel J,Sunter D,Tuck E,Hooks Y,Carragher DM,Clarke K,Karp NA,,Newbigging S,Jones N,Morikawa L,White JK,McKerlie C


book

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Development (Cambridge, England)140(22)4633-44(2013 Nov)
Miller JA,Nathanson J,Franjic D,Shim S,Dalley RA,Shapouri S,Smith KA,Sunkin SM,Bernard A,Bennett JL,Lee CK,Hawrylycz MJ,Jones AR,Amaral DG,?estan N,Gage FH,Lein ES