Axin1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Axin1
Entrez Gene ID	12005
Full Name	axin 1
Synonyms	AI316800,Axin,Fu,Kb,Ki,fused,kinky,knobbly
General protein information	Preferred Names axin 1 Names axin-1 axis inhibition protein 1 protein Fused
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 13.07 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_030249451.1	XP_030105311.1	axin-1 isoform X2
XM_030249452.1	XP_030105312.1	axin-1 isoform X2
XM_006523515.3	XP_006523578.1	axin-1 isoform X1
NM_009733.2	NP_033863.2	axin-1 isoform 2
XM_006523515.4	XP_006523578.1	axin-1 isoform X1
XM_006523516.2	XP_006523579.1	axin-1 isoform X3
NM_001159598.1	NP_001153070.1	axin-1 isoform 1

Pathways from BioSystems

Homologies

	AXIN1	XP_002802391.1
Danio rerio (zebrafish)	axin1	NP_571578.2
Pan troglodytes (chimpanzee)	AXIN1	XP_001152990.3
Canis lupus familiaris (dog)	AXIN1	XP_852321.3
Bos taurus (cattle)	AXIN1	NP_001178327.1
Mus musculus (house mouse)	Axin1	NP_033863.2
Rattus norvegicus (Norway rat)	Axin1	NP_077381.1
Gallus gallus (chicken)	AXIN1	NP_990275.1
Homo sapiens (human)	AXIN1	NP_003493.1
Xenopus tropicalis (tropical clawed frog)	axin1	XP_002935881.1

Gene Ontology
Bibliography

Related articles in PubMed

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.
Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC
Nature548(7665)112-116(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VDR is important for the maintenance of physiological level of Axin1
Title: Vitamin D receptor is a novel transcriptional regulator for Axin1.

Axin-1 is critical for spindle organization and cell cycle progression during meiotic maturation in mouse oocytes.
Title: Axin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes.

Axin1 and Axin2 do not have equivalent functions in satellite cells, but are both involved in repression of Wnt/beta-catenin signalling to maintain proliferation and contribute to controlling timely myogenic differentiation.
Title: Coordinated action of Axin1 and Axin2 suppresses ?-catenin to regulate muscle stem cell function.

Study reports that NRF2 participates in the formation of a protein complex with Axin1/GSK-3/beta-TrCP that is disrupted by WNT-3A, a prototypic canonical WNT ligand, and leads to upregulation of the NRF2 transcriptional signature.
Title: WNT-3A regulates an Axin1/NRF2 complex that regulates antioxidant metabolism in hepatocytes.

Axin1 promotes dendritic spine stabilization through Cdc42-dependent cytoskeletal reorganization.
Title: Axin Regulates Dendritic Spine Morphogenesis through Cdc42-Dependent Signaling.

The following Axin1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Axin1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu53032	XM_006523516.2 Latest version!	Mus musculus axin 1 (Axin1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu18295	NM_001159598.1 Latest version!	Mus musculus axin 1 (Axin1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$489.30 $699.00
OMu22495	NM_009733.2 Latest version!	Mus musculus axin 1 (Axin1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu18295	XM_030249451.1 Latest version!	Mus musculus axin 1 (Axin1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu18295	XM_030249452.1 Latest version!	Mus musculus axin 1 (Axin1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu22495	XM_006523515.4 Latest version!	Mus musculus axin 1 (Axin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu22495	XM_006523515.3	Mus musculus axin 1 (Axin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Axin1 ( XM_006523516.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523579.1 Mus musculus axin 1 (Axin1), transcript variant X4, mRNA.

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CloneID	OMu53032
Clone ID Related Accession (Same CDS sequence)	XM_006523516.2
Accession Version	XM_006523516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	axin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523579.1
CDS	272..1798
Translation	MQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	XM_006523516.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523516.2

ORF Insert Sequence:

ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axin1 ( NM_001159598.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153070.1 Mus musculus axin 1 (Axin1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18295
Clone ID Related Accession (Same CDS sequence)	XM_030249451.1 , XM_030249452.1 , NM_001159598.1
Accession Version	NM_001159598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	axin-1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC140047.8 and AC126438.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC113171.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGACAAA ATCACAAAGA 
AAGGCAGGTG GCGGGAGTGC ACCACCATGT GACAGCATTG TTGTGGCCTA CTATTTCTGT 
GGGGAACCCA TCCCCTACCG GACCCTGGTG AGGGGCCGTG CTGTCACCCT GGGCCAGTTC 
AAGGAGCTGC TAACCAAGAA GGGGAGCTAC AGATACTACT TTAAGAAAGT GAGTGATGAG 
TTTGACTGTG GTGTGGTATT TGAGGAAGTA CGGGAGGATG AGGCCGTCTT GCCTGTCTTT 
GAAGAAAAGA TCATCGGCAA GGTGGAAAAG GTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153070.1
CDS	423..2921
Translation	MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	NM_001159598.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159598.1

ORF Insert Sequence:

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGACAAA ATCACAAAGA 
AAGGCAGGTG GCGGGAGTGC ACCACCATGT GACAGCATTG TTGTGGCCTA CTATTTCTGT 
GGGGAACCCA TCCCCTACCG GACCCTGGTG AGGGGCCGTG CTGTCACCCT GGGCCAGTTC 
AAGGAGCTGC TAACCAAGAA GGGGAGCTAC AGATACTACT TTAAGAAAGT GAGTGATGAG 
TTTGACTGTG GTGTGGTATT TGAGGAAGTA CGGGAGGATG AGGCCGTCTT GCCTGTCTTT 
GAAGAAAAGA TCATCGGCAA GGTGGAAAAG GTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axin1 ( NM_009733.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033863.2 Mus musculus axin 1 (Axin1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22495
Clone ID Related Accession (Same CDS sequence)	XM_006523515.3 , NM_009733.2 , XM_006523515.4
Accession Version	NM_009733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Mus musculus(house mouse)
Product	axin-1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ060582.1, AF009011.1, BG803725.1, AA259709.1 and AW061082.1. On Apr 23, 2009 this sequence version replaced NM_009733.1. Transcript Variant: This variant contains an alternate in-frame exon in the 3' coding region compared to variant 1, resulting in a longer protein (isoform 2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF009011.1, BC011544.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033863.2
CDS	423..3029
Translation	MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	NM_009733.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009733.2

ORF Insert Sequence:

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axin1 ( XM_006523515.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523578.1 Mus musculus axin 1 (Axin1), transcript variant X1, mRNA.

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CloneID	OMu22495
Clone ID Related Accession (Same CDS sequence)	XM_006523515.3 , NM_009733.2 , XM_006523515.4
Accession Version	XM_006523515.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	axin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523515.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523578.1
CDS	451..3057
Translation	MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	XM_006523515.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523515.3

ORF Insert Sequence:

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axin1 ( XM_030249451.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105311.1 Mus musculus axin 1 (Axin1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu18295
Clone ID Related Accession (Same CDS sequence)	XM_030249451.1 , XM_030249452.1 , NM_001159598.1
Accession Version	XM_030249451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	axin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGACAAA ATCACAAAGA 
AAGGCAGGTG GCGGGAGTGC ACCACCATGT GACAGCATTG TTGTGGCCTA CTATTTCTGT 
GGGGAACCCA TCCCCTACCG GACCCTGGTG AGGGGCCGTG CTGTCACCCT GGGCCAGTTC 
AAGGAGCTGC TAACCAAGAA GGGGAGCTAC AGATACTACT TTAAGAAAGT GAGTGATGAG 
TTTGACTGTG GTGTGGTATT TGAGGAAGTA CGGGAGGATG AGGCCGTCTT GCCTGTCTTT 
GAAGAAAAGA TCATCGGCAA GGTGGAAAAG GTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105311.1
CDS	444..2942
Translation	MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	XM_030249451.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249451.1

ORF Insert Sequence:

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGACAAA ATCACAAAGA 
AAGGCAGGTG GCGGGAGTGC ACCACCATGT GACAGCATTG TTGTGGCCTA CTATTTCTGT 
GGGGAACCCA TCCCCTACCG GACCCTGGTG AGGGGCCGTG CTGTCACCCT GGGCCAGTTC 
AAGGAGCTGC TAACCAAGAA GGGGAGCTAC AGATACTACT TTAAGAAAGT GAGTGATGAG 
TTTGACTGTG GTGTGGTATT TGAGGAAGTA CGGGAGGATG AGGCCGTCTT GCCTGTCTTT 
GAAGAAAAGA TCATCGGCAA GGTGGAAAAG GTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axin1 ( XM_030249452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030105312.1 Mus musculus axin 1 (Axin1), transcript variant X3, mRNA.

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CloneID	OMu18295
Clone ID Related Accession (Same CDS sequence)	XM_030249451.1 , XM_030249452.1 , NM_001159598.1
Accession Version	XM_030249452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	axin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGACAAA ATCACAAAGA 
AAGGCAGGTG GCGGGAGTGC ACCACCATGT GACAGCATTG TTGTGGCCTA CTATTTCTGT 
GGGGAACCCA TCCCCTACCG GACCCTGGTG AGGGGCCGTG CTGTCACCCT GGGCCAGTTC 
AAGGAGCTGC TAACCAAGAA GGGGAGCTAC AGATACTACT TTAAGAAAGT GAGTGATGAG 
TTTGACTGTG GTGTGGTATT TGAGGAAGTA CGGGAGGATG AGGCCGTCTT GCCTGTCTTT 
GAAGAAAAGA TCATCGGCAA GGTGGAAAAG GTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030105312.1
CDS	439..2937
Translation	MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	XM_030249452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030249452.1

ORF Insert Sequence:

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGACAAA ATCACAAAGA 
AAGGCAGGTG GCGGGAGTGC ACCACCATGT GACAGCATTG TTGTGGCCTA CTATTTCTGT 
GGGGAACCCA TCCCCTACCG GACCCTGGTG AGGGGCCGTG CTGTCACCCT GGGCCAGTTC 
AAGGAGCTGC TAACCAAGAA GGGGAGCTAC AGATACTACT TTAAGAAAGT GAGTGATGAG 
TTTGACTGTG GTGTGGTATT TGAGGAAGTA CGGGAGGATG AGGCCGTCTT GCCTGTCTTT 
GAAGAAAAGA TCATCGGCAA GGTGGAAAAG GTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axin1 ( XM_006523515.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523578.1 Mus musculus axin 1 (Axin1), transcript variant X1, mRNA.

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CloneID	OMu22495
Clone ID Related Accession (Same CDS sequence)	XM_006523515.3 , NM_009733.2 , XM_006523515.4
Accession Version	XM_006523515.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	axin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523515.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523578.1
CDS	447..3053
Translation	MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD

Target ORF information:

RefSeq Version	XM_006523515.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus axin 1 (Axin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523515.4

ORF Insert Sequence:

ATGCAGAGTC CCAAAATGAA TGTCCAGGAG CAGGGTTTCC CCTTGGACCT CGGAGCAAGT 
TTCACCGAAG ATGCCCCCCG GCCCCCAGTG CCTGGAGAAG AGGGAGAACT GGTATCTACT 
GATTCGAGGC CTGTCAACCA CAGTTTCTGT TCTGGGAAAG GTACCAGCAT TAAAAGTGAG 
ACCTCAACAG CCACCCCAAG ACGTTCAGAT CTGGATCTGG GATATGAGCC CGAGGGCAGT 
GCCTCCCCCA CCCCACCATA TTTGAGGTGG GCTGAGTCAC TGCATTCCTT ACTGGATGAC 
CAAGATGGGA TCAGCCTGTT CAGGACTTTC CTGAAGCAGG AGGGCTGTGC TGACCTGCTG 
GACTTCTGGT TTGCCTGCAG TGGCTTCAGG AAGCTTGAGC CCTGTGACTC AAATGAGGAA 
AAGAGGCTGA AGCTGGCAAG AGCCATCTAC CGAAAGTACA TCCTGGATAG CAATGGCATT 
GTGTCCAGAC AAACCAAGCC AGCCACTAAG AGCTTCATAA AGGACTGTGT CATGAAGCAG 
CAGATAGATC CTGCCATGTT TGACCAGGCA CAGACAGAAA TCCAGTCCAC CATGGAGGAG 
AATACCTACC CTTCCTTTCT TAAGTCTGAC ATTTATTTGG AGTACACAAG GACAGGCTCA 
GAGAGTCCGA AGGTCTGCAG TGACCAGAGC TCAGGGTCTG GAACAGGGAA GGGCATGTCT 
GGATACCTGC CCACTTTGAA TGAGGATGAA GAATGGAAAT GTGACCAAGA TGCAGATGAG 
GATGATGGCC GAGACCCTCT CCCCCCCAGC AGGCTCACCC AGAAGCTGCT ATTGGAGACT 
GCTGCCCCGA GGGCCCCCTC AAGTAGACGG TACAACGAAG GCAGAGAGCT CAGGTATGGA 
TCTTGGAGGG AGCCCGTCAA CCCCTACTAC GTCAACTCTG GCTATGCCCT GGCCCCAGCC 
ACCAGTGCCA ATGACAGTGA GCAGCAGAGC CTGTCCAGTG ATGCTGACAC GCTATCCCTT 
ACGGACAGTA GTGTGGATGG AATCCCCCCA TACAGGATCC GTAAGCAGCA CCGAAGGGAG 
ATGCAGGAGA GTATCCAAGT CAATGGGCGG GTACCTCTAC CTCACATTCC TCGCACTTAC 
CGAATGCCAA AGGAGATCCG GGTAGAGCCA CAGAAATTTG CTGAAGAGCT TATTCACCGT 
CTAGAGGCTG TCCAGCGCAC TCGAGAGGCT GAAGAAAAGT TGGAGGAACG GCTGAAGCGT 
GTACGCATGG AGGAAGAAGG GGAGGATGGT GAAATGCCTT CTGGCCCCAT GGCAAGTCAC 
AAGCTGCCTT CTGTCCCAGC TTGGCACCAT TTCCCACCCC GCTATGTGGA TATGGGCTGC 
TCTGGACTGC GGGATGCCCA TGAGGAGAAT CCTGAGAGCA TCCTGGATGA GCACGTGCAA 
AGGGTCATGA GGACACCTGG CTGCCAGTCA CCTGGCCCAG GCCACCGCTC TCCTGACAGT 
GGGCATGTGG CTAAGACTGC AGTGCTAGGG GGTACAGCCT CCGGGCATGG GAAGCATGTT 
CCTAAGTTAG GGTTGAAGCT GGATACAGCT GGCCTGCACC ATCATAGACA TGTCCACCAC 
CATGTTCACC ATAATTCAGC TAGACCTAAG GAGCAAATGG AGGCTGAAGT TGCCCGCAGG 
GTCCAGAGCA GCTTCTCGTG GGGCCCAGAA ACACATGGTC ATGCCAAGCC CCGGAGCTAT 
TCCGAGAACG CAGGCACCAC CCTCAGTGCT GGGGATTTGG CCTTTGGTGG TAAAACTAGT 
GCACCTTCCA AAAGAAACAC CAAGAAGGCT GAATCTGGGA AGAATGCCAA TGCTGAGGTA 
CCCAGTACCA CAGAGGACGC TGAGAAGAAC CAGAAGATCA TGCAGTGGAT CATTGAGGGA 
GAGAAGGAGA TCAGTAGACA CCGGAAGGCA GGCCATGGGT CTTCTGGGTT GAGGAAGCAG 
CAGGCCCATG AAAGCTCCAG GCCCTTGTCC ATCGAGCGTC CTGGGGCCGT GCACCCCTGG 
GTCAGCGCTC AGCTTCGGAA TTCTGTCCAG CCTTCTCATC TTTTCATCCA AGATCCCACA 
ATGCCACCCA ATCCAGCCCC TAATCCCCTG ACCCAGCTGG AAGAGGCCCG CAGGCGTTTG 
GAAGAAGAAG AAAAGAGAGC AAACAAACTG CCCTCCAAGC AGAGGTATGT GCAGGCAGTC 
ATGCAGCGGG GACGCACCTG TGTCAGGCCA GCTTGTGCAC CGGTGCTGAG TGTGGTACCA 
GCCGTGTCGG ACTTGGAACT CTCCGAGACA GAGACAAAAT CACAAAGAAA GGCAGGTGGC 
GGGAGTGCAC CACCATGTGA CAGCATTGTT GTGGCCTACT ATTTCTGTGG GGAACCCATC 
CCCTACCGGA CCCTGGTGAG GGGCCGTGCT GTCACCCTGG GCCAGTTCAA GGAGCTGCTA 
ACCAAGAAGG GGAGCTACAG ATACTACTTT AAGAAAGTGA GTGATGAGTT TGACTGTGGT 
GTGGTATTTG AGGAAGTACG GGAGGATGAG GCCGTCTTGC CTGTCTTTGA AGAAAAGATC 
ATCGGCAAGG TGGAAAAGGT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.
Nature548(7665)112-116(2017 08)
Zhang CS,Hawley SA,Zong Y,Li M,Wang Z,Gray A,Ma T,Cui J,Feng JW,Zhu M,Wu YQ,Li TY,Ye Z,Lin SY,Yin H,Piao HL,Hardie DG,Lin SC

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Axin1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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