Gene Symbol | Bcat1 |
Entrez Gene ID | 12035 |
Full Name | branched chain aminotransferase 1, cytosolic |
Synonyms | BCATc,Eca39 |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Bcat1 cDNA ORF clone
Gene Symbol | Bcat1 |
Entrez Gene ID | 12035 |
Full Name | branched chain aminotransferase 1, cytosolic |
Synonyms | BCATc,Eca39 |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_011241566.3 | XP_011239868.1 | branched-chain-amino-acid aminotransferase, cytosolic isoform X1 |
XM_011241566.2 | XP_011239868.1 | branched-chain-amino-acid aminotransferase, cytosolic isoform X1 |
NM_001024468.3 | NP_001019639.1 | branched-chain-amino-acid aminotransferase, cytosolic isoform 1 |
NM_007532.5 | NP_031558.3 | branched-chain-amino-acid aminotransferase, cytosolic isoform 2 |
Bos taurus (cattle) | BCAT1 | NP_001077113.1 |
Mus musculus (house mouse) | Bcat1 | NP_001019639.1 |
Caenorhabditis elegans (roundworm) | bcat-1 | NP_510144.1 |
Oryza sativa (rice) | Os05g0558400 | NP_001056295.1 |
Pan troglodytes (chimpanzee) | BCAT1 | XP_001143074.2 |
BCAT1 | XP_001101021.2 | |
Danio rerio (zebrafish) | bcat1 | NP_956358.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP000011 | XP_311149.3 |
Oryza sativa (rice) | Os03g0106400 | NP_001048685.1 |
Canis lupus familiaris (dog) | BCAT1 | XP_005637110.1 |
Gallus gallus (chicken) | BCAT1 | XP_004938031.1 |
Xenopus tropicalis (tropical clawed frog) | bcat1 | NP_001096227.1 |
Drosophila melanogaster (fruit fly) | CG1673 | NP_572884.1 |
Arabidopsis thaliana (thale cress) | ATBCAT-5 | NP_201379.2 |
Arabidopsis thaliana (thale cress) | BCAT3 | NP_566923.1 |
Homo sapiens (human) | BCAT1 | NP_001171564.1 |
Rattus norvegicus (Norway rat) | Bcat1 | NP_058949.1 |
Saccharomyces cerevisiae (baker's yeast) | BAT2 | NP_012682.1 |
Eremothecium gossypii | AGOS_ADL384W | NP_983712.1 |
BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases.
Papathanassiu AE, Ko JH, Imprialou M, Bagnati M, Srivastava PK, Vu HA, Cucchi D, McAdoo SP, Ananieva EA, Mauro C, Behmoaras J
Nature communications816040(2017 Jul)
Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism.
Koike H, Zhang RR, Ueno Y, Sekine K, Zheng YW, Takebe T, Taniguchi H
Development (Cambridge, England)144(6)1018-1024(2017 03)
Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse.
Yu C, Ji SY, Dang YJ, Sha QQ, Yuan YF, Zhou JJ, Yan LY, Qiao J, Tang F, Fan HY
Cell research26(3)275-87(2016 Mar)
Global deletion of BCATm increases expression of skeletal muscle genes associated with protein turnover.
Lynch CJ, Kimball SR, Xu Y, Salzberg AC, Kawasawa YI
Physiological genomics47(11)569-80(2015 Nov)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
results suggest transcriptional adaptations occur in BCATm KO mice that along with altered nutrient signaling may contribute to their previously reported protein turnover, metabolic and exercise phenotypes
Title: Global deletion of BCATm increases expression of skeletal muscle genes associated with protein turnover.
BCATc as a novel regulator of T cell activation and metabolism
Title: Cytosolic branched chain aminotransferase (BCATc) regulates mTORC1 signaling and glycolytic metabolism in CD4+ T cells.
leucine supplementation increased the expression of enzymes (BCAT1, BCAT2 and BCKDK) that metabolize branched-chain amino acids.
Title: Oral leucine supplementation is sensed by the brain but neither reduces food intake nor induces an anorectic pattern of gene expression in the hypothalamus.
analysis of the biochemical mechanism of BCATm (branched-chain aminotransferase) catalysis of reversible transamination of leucine and alpha-ketoglutarate to KIC and glutamate
Title: Transamination is required for {alpha}-ketoisocaproate but not leucine to stimulate insulin secretion.
BCATm(-/-) mice had elevated plasma branched-chain amino acids & decreased adiposity & body weight, despite eating more food, along with increased energy expenditure, improvements in glucose & insulin tolerance & protection from diet-induced obesity
Title: Disruption of BCATm in mice leads to increased energy expenditure associated with the activation of a futile protein turnover cycle.
The following Bcat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bcat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu00341 | |
Clone ID Related Accession (Same CDS sequence) | NM_007532.5 | |
Accession Version | NM_007532.5 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-27 | |
Organism | Mus musculus(house mouse) | |
Product | branched-chain-amino-acid aminotransferase, cytosolic isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY781109.1, AK167232.1, AC140416.2 and AW491045.1. On Oct 15, 2008 this sequence version replaced NM_007532.4. Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, compared to variant 1. The resulting protein (isoform 2) has a shorter and unique N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK168249.1, AK166971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGAAGGACT GCAGTAATGG ATGCTCCGCG CCGTTTGCTG GAGAAAGAGG ATCAGAAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_031558.3 |
CDS | 264..1424 |
Translation |
Target ORF information:
Target ORF information:
|
NM_007532.5 |
1 | ATGAAGGACT GCAGTAATGG ATGCTCCGCG CCGTTTGCTG GAGAAAGAGG ATCAGAAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu11838 | |
Clone ID Related Accession (Same CDS sequence) | NM_001024468.3 | |
Accession Version | NM_001024468.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-21 | |
Organism | Mus musculus(house mouse) | |
Product | branched-chain-amino-acid aminotransferase, cytosolic isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK036309.1, AC140416.2 and AW491045.1. On Oct 15, 2008 this sequence version replaced NM_001024468.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036309.1, AK013888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## |
1 | ATGCTGCATC GGCTGCGCTC GCCCCTGCGC CGGGTGCTTG CTGGTGGCTC TCCCATCGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001019639.1 |
CDS | 44..1405 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001024468.3 |
1 | ATGCTGCATC GGCTGCGCTC GCCCCTGCGC CGGGTGCTTG CTGGTGGCTC TCCCATCGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu67196 | |
Clone ID Related Accession (Same CDS sequence) | XM_011241566.3 , XM_011241566.2 | |
Accession Version | XM_011241566.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | branched-chain-amino-acid aminotransferase, cytosolic isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACTGCA GTAATGGATG CTCCGCGCCG TTTGCTGGAG AAAGAGGATC AGAAGAAGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011239868.1 |
CDS | 608..1765 |
Translation |
Target ORF information:
Target ORF information:
|
XM_011241566.2 |
1 | ATGGACTGCA GTAATGGATG CTCCGCGCCG TTTGCTGGAG AAAGAGGATC AGAAGAAGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu67196 | |
Clone ID Related Accession (Same CDS sequence) | XM_011241566.3 , XM_011241566.2 | |
Accession Version | XM_011241566.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | branched-chain-amino-acid aminotransferase, cytosolic isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241566.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGACTGCA GTAATGGATG CTCCGCGCCG TTTGCTGGAG AAAGAGGATC AGAAGAAGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011239868.1 |
CDS | 983..2140 |
Translation |
Target ORF information:
Target ORF information:
|
XM_011241566.3 |
1 | ATGGACTGCA GTAATGGATG CTCCGCGCCG TTTGCTGGAG AAAGAGGATC AGAAGAAGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases. |
Nutritional modulation of mouse and human liver bud growth through a branched-chain amino acid metabolism. |
Oocyte-expressed yes-associated protein is a key activator of the early zygotic genome in mouse. |
Global deletion of BCATm increases expression of skeletal muscle genes associated with protein turnover. |