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Capns1 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Capns1
Entrez Gene ID 12336
Full Name calpain, small subunit 1
Synonyms Capa-4,Capa4,Capn4,Cdps,Css1,D7Ertd146e
General protein information
Preferred Names

calpain, small subunit 1

Names

calpain small subunit 1
CANP small subunit
calcium-activated neutral proteinase small subunit
calcium-dependent protease small subunit 1
calpain 4
calpain regulatory subunit
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

7

7 B1|7 17.31 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006539488.2 XP_006539551.1 calpain small subunit 1 isoform X1
XM_011250419.2 XP_011248721.1 calpain small subunit 1 isoform X2
XM_011250419.3 XP_011248721.1 calpain small subunit 1 isoform X2
NM_009795.4 NP_033925.2 calpain small subunit 1
NM_009795.3 NP_033925.2 calpain small subunit 1
XM_006539488.3 XP_006539551.1 calpain small subunit 1 isoform X1
XM_011250420.1 XP_011248722.1 calpain small subunit 1 isoform X3

Pathways from BioSystems


Homologies


LOC712561 XP_002808235.1
Rattus norvegicus (Norway rat) LOC100911363 XP_003748884.1
Danio rerio (zebrafish) capns1a NP_001017899.2
Danio rerio (zebrafish) capns1b NP_001103176.1
Homo sapiens (human) CAPNS1 NP_001003962.1
Pan troglodytes (chimpanzee) CAPNS1 XP_003953505.1
Xenopus tropicalis (tropical clawed frog) capns2 NP_001016161.1
Canis lupus familiaris (dog) CAPNS1 XP_853634.1
Mus musculus (house mouse) Capns1 NP_033925.2
Rattus norvegicus (Norway rat) Capns1 NP_058814.1
Bos taurus (cattle) CAPNS1 NP_776686.1
Rattus norvegicus (Norway rat) LOC100912380 XP_003748875.2

Gene Ontology


Bibliography

Related articles in PubMed

Disruption of calpain reduces lipotoxicity-induced cardiac injury by preventing endoplasmic reticulum stress.
Li S, Zhang L, Ni R, Cao T, Zheng D, Xiong S, Greer PA, Fan GC, Peng T
Biochimica et biophysica acta1862(11)2023-2033(2016 Nov)

Calpain Genetic Disruption and HSP90 Inhibition Combine To Attenuate Mammary Tumorigenesis.
Grieve S, Gao Y, Hall C, Hu J, Greer PA
Molecular and cellular biology36(15)2078-88(2016 Aug)

Calpain-2 activates Akt via TGF-?1-mTORC2 pathway in pulmonary artery smooth muscle cells.
Abeyrathna P, Kovacs L, Han W, Su Y
American journal of physiology. Cell physiology311(1)C24-34(2016 Jul)

Activation of Calpain-2 by Mediators in Pulmonary Vascular Remodeling of Pulmonary Arterial Hypertension.
Kovacs L, Han W, Rafikov R, Bagi Z, Offermanns S, Saido TC, Black SM, Su Y
American journal of respiratory cell and molecular biology54(3)384-93(2016 Mar)

Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy.
Ni R, Zheng D, Xiong S, Hill DJ, Sun T, Gardiner RB, Fan GC, Lu Y, Abel ED, Greer PA, Peng T
Diabetes65(1)255-68(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mitochondrial calpain-1 disrupts ATP synthase, leading to mitochondrial reactive oxygen species generation, which promotes proinflammatory response and myocardial dysfunction during endotoxemia.
Title: Deletion of capn4 Protects the Heart Against Endotoxemic Injury by Preventing ATP Synthase Disruption and Inhibiting Mitochondrial Superoxide Generation.

Calpain in osteoblasts regulates cell proliferation through its regulation of p27Kip1. Osteoblast-specific capns1 knockout mice developed a high-fat diet-induced excess body weight gain and impaired glucose tolerance.
Title: Overview of calpain-mediated regulation of bone and fat mass in osteoblasts.

effects of Capn4 deletion may be mediated through prevention of IkappaB degradation and NF-kappaB activation, resulting in inhibition of inflammatory responses.
Title: Deficiency of Capn4 gene inhibits nuclear factor-?B (NF-?B) protein signaling/inflammation and reduces remodeling after myocardial infarction.

osteoblast-specific Capns1 knockout mice with osteoporosis and reduced bone resorptive function, exhibited reduced serum uncarboxylated osteocalcin levels and a lean phenotype under a normal chow diet.
Title: A high fat diet-induced impaired glucose metabolism in mice with targeted deletion of calpain in osteoblasts.

Calpain 4 is essential for the tyrosine phosphorylation of galectin-3, and its ultimate secretion from the cell, and its secretion probably interferes with the production of traction forces.
Title: Galectin-3 secretion and tyrosine phosphorylation is dependent on the calpain small subunit, Calpain 4.

The following Capns1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capns1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu67294 XM_011250420.1
Latest version!		 Mus musculus calpain, small subunit 1 (Capns1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $69.30
$99.00
OMu22049 NM_009795.4
Latest version!		 Mus musculus calpain, small subunit 1 (Capns1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu22049 XM_006539488.3
Latest version!		 Mus musculus calpain, small subunit 1 (Capns1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OMu67293 XM_011250419.3
Latest version!		 Mus musculus calpain, small subunit 1 (Capns1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu67293 XM_011250419.2 Mus musculus calpain, small subunit 1 (Capns1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OMu22049 NM_009795.3 Mus musculus calpain, small subunit 1 (Capns1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu22049 XM_006539488.2 Mus musculus calpain, small subunit 1 (Capns1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu67294
Clone ID Related Accession (Same CDS sequence) XM_011250420.1
Accession Version XM_011250420.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 471bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product calpain small subunit 1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
ATGGAGGTCA GCGCCACAGA ACTCATGAAT ATCCTCAACA AGGTCGTGAC CCGACACCCG 
GATCTGAAAA CTGATGGTTT TGGCATTGAC ACTTGTCGGA GCATGGTGGC CGTCATGGAT 
AGCGACACCA CAGGCAAGCT GGGCTTTGAG GAGTTCAAAT ACCTCTGGAA CAACATTAAA 
AAGTGGCAGG CTATATACAA ACGCTTTGAT ACTGACCGAT CAGGGACTAT CGGTAGCCAT 
GAACTTCCAG GGGCCTTTGA GGCAGCAGGA TTCCACCTGA ATGAGCATCT CTACAGCATG 
ATCATCCGGC GCTACGCAGA TGAGAGCGGG AACATGGATT TCGATAACTT CATCAGCTGC 
TTGGTCAGGC TAGATGCCAT GTTCCGCGCT TTCAAATCTC TTGACAAAAA TGGCACCGGA 
CAGATCCAAG TGAACATCCA GGAGTGGCTG CAGCTGACTA TGTATTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248722.1
CDS630..1100
Translation

Target ORF information:

RefSeq Version XM_011250420.1
Organism Mus musculus(house mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250420.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGAGGTCA GCGCCACAGA ACTCATGAAT ATCCTCAACA AGGTCGTGAC CCGACACCCG 
GATCTGAAAA CTGATGGTTT TGGCATTGAC ACTTGTCGGA GCATGGTGGC CGTCATGGAT 
AGCGACACCA CAGGCAAGCT GGGCTTTGAG GAGTTCAAAT ACCTCTGGAA CAACATTAAA 
AAGTGGCAGG CTATATACAA ACGCTTTGAT ACTGACCGAT CAGGGACTAT CGGTAGCCAT 
GAACTTCCAG GGGCCTTTGA GGCAGCAGGA TTCCACCTGA ATGAGCATCT CTACAGCATG 
ATCATCCGGC GCTACGCAGA TGAGAGCGGG AACATGGATT TCGATAACTT CATCAGCTGC 
TTGGTCAGGC TAGATGCCAT GTTCCGCGCT TTCAAATCTC TTGACAAAAA TGGCACCGGA 
CAGATCCAAG TGAACATCCA GGAGTGGCTG CAGCTGACTA TGTATTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu67293
Clone ID Related Accession (Same CDS sequence) XM_011250419.2 , XM_011250419.3
Accession Version XM_011250419.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 642bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product calpain small subunit 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGAGGGAA AGGAGGGGTC AGACCATTCT CTTGATTGGA CCAGCGAGGC TGCTGCGCAG 
TACAACCCGG AGCCTCCGCC TCCACGTAGT CATTACTCCA ATATTGAGGC CAACGAGAGT 
GAAGAAGTCC GTCAGTTCAG GAAACTTTTT GTCCAGCTGG CTGGAGACGA CATGGAGGTC 
AGCGCCACAG AACTCATGAA TATCCTCAAC AAGGTCGTGA CCCGACACCC GGATCTGAAA 
ACTGATGGTT TTGGCATTGA CACTTGTCGG AGCATGGTGG CCGTCATGGA TAGCGACACC 
ACAGGCAAGC TGGGCTTTGA GGAGTTCAAA TACCTCTGGA ACAACATTAA AAAGTGGCAG 
GCTATATACA AACGCTTTGA TACTGACCGA TCAGGGACTA TCGGTAGCCA TGAACTTCCA 
GGGGCCTTTG AGGCAGCAGG ATTCCACCTG AATGAGCATC TCTACAGCAT GATCATCCGG 
CGCTACGCAG ATGAGAGCGG GAACATGGAT TTCGATAACT TCATCAGCTG CTTGGTCAGG 
CTAGATGCCA TGTTCCGCGC TTTCAAATCT CTTGACAAAA ATGGCACCGG ACAGATCCAA 
GTGAACATCC AGGAGTGGCT GCAGCTGACT ATGTATTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248721.1
CDS115..756
Translation

Target ORF information:

RefSeq Version XM_011250419.2
Organism Mus musculus(house mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250419.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGGGAA AGGAGGGGTC AGACCATTCT CTTGATTGGA CCAGCGAGGC TGCTGCGCAG 
TACAACCCGG AGCCTCCGCC TCCACGTAGT CATTACTCCA ATATTGAGGC CAACGAGAGT 
GAAGAAGTCC GTCAGTTCAG GAAACTTTTT GTCCAGCTGG CTGGAGACGA CATGGAGGTC 
AGCGCCACAG AACTCATGAA TATCCTCAAC AAGGTCGTGA CCCGACACCC GGATCTGAAA 
ACTGATGGTT TTGGCATTGA CACTTGTCGG AGCATGGTGG CCGTCATGGA TAGCGACACC 
ACAGGCAAGC TGGGCTTTGA GGAGTTCAAA TACCTCTGGA ACAACATTAA AAAGTGGCAG 
GCTATATACA AACGCTTTGA TACTGACCGA TCAGGGACTA TCGGTAGCCA TGAACTTCCA 
GGGGCCTTTG AGGCAGCAGG ATTCCACCTG AATGAGCATC TCTACAGCAT GATCATCCGG 
CGCTACGCAG ATGAGAGCGG GAACATGGAT TTCGATAACT TCATCAGCTG CTTGGTCAGG 
CTAGATGCCA TGTTCCGCGC TTTCAAATCT CTTGACAAAA ATGGCACCGG ACAGATCCAA 
GTGAACATCC AGGAGTGGCT GCAGCTGACT ATGTATTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22049
Clone ID Related Accession (Same CDS sequence) XM_006539488.2 , NM_009795.4 , NM_009795.3 , XM_006539488.3
Accession Version NM_009795.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 807bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-28
Organism Mus musculus(house mouse)
Product calpain small subunit 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY133208.1, BY296628.1, BC090988.1 and BU700497.1. On Jan 19, 2017 this sequence version replaced NM_009795.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC090988.1, AF058298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_033925.2
CDS164..970
Translation

Target ORF information:

RefSeq Version NM_009795.4
Organism Mus musculus(house mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_009795.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22049
Clone ID Related Accession (Same CDS sequence) XM_006539488.2 , NM_009795.4 , NM_009795.3 , XM_006539488.3
Accession Version NM_009795.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 807bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-02-14
Organism Mus musculus(House mouse)
Product calpain small subunit 1
Comment 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_033925.2
Translation

Target ORF information:

RefSeq Version NM_009795.3
Organism Mus musculus(House mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_009795.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22049
Clone ID Related Accession (Same CDS sequence) XM_006539488.2 , NM_009795.4 , NM_009795.3 , XM_006539488.3
Accession Version XM_006539488.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 807bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product calpain small subunit 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006539488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539551.1
CDS212..1018
Translation

Target ORF information:

RefSeq Version XM_006539488.2
Organism Mus musculus(house mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539488.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu22049
Clone ID Related Accession (Same CDS sequence) XM_006539488.2 , NM_009795.4 , NM_009795.3 , XM_006539488.3
Accession Version XM_006539488.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 807bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product calpain small subunit 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539488.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006539551.1
CDS301..1107
Translation

Target ORF information:

RefSeq Version XM_006539488.3
Organism Mus musculus(house mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006539488.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGTTCTTGG TGAATTCGTT CTTGAAGGGC GGCGGTGGCG GTGGGGGAGG CGGGGGTCTG 
GGCGGGGGCC TGGGGAATGT GCTCGGAGGC CTGATCAGCG GAGCTGCTGG CGGCGGCGGC 
GGTGGCGGTG GAGGCATGGG ACTGGGAGGA GGTGGCGGAG GCGGTGGAAC TGCCATGCGC 
ATCCTGGGCG GAGTCATTAG CGCCATCAGC GAGGCTGCTG CGCAGTACAA CCCGGAGCCT 
CCGCCTCCAC GTAGTCATTA CTCCAATATT GAGGCCAACG AGAGTGAAGA AGTCCGTCAG 
TTCAGGAAAC TTTTTGTCCA GCTGGCTGGA GACGACATGG AGGTCAGCGC CACAGAACTC 
ATGAATATCC TCAACAAGGT CGTGACCCGA CACCCGGATC TGAAAACTGA TGGTTTTGGC 
ATTGACACTT GTCGGAGCAT GGTGGCCGTC ATGGATAGCG ACACCACAGG CAAGCTGGGC 
TTTGAGGAGT TCAAATACCT CTGGAACAAC ATTAAAAAGT GGCAGGCTAT ATACAAACGC 
TTTGATACTG ACCGATCAGG GACTATCGGT AGCCATGAAC TTCCAGGGGC CTTTGAGGCA 
GCAGGATTCC ACCTGAATGA GCATCTCTAC AGCATGATCA TCCGGCGCTA CGCAGATGAG 
AGCGGGAACA TGGATTTCGA TAACTTCATC AGCTGCTTGG TCAGGCTAGA TGCCATGTTC 
CGCGCTTTCA AATCTCTTGA CAAAAATGGC ACCGGACAGA TCCAAGTGAA CATCCAGGAG 
TGGCTGCAGC TGACTATGTA TTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu67293
Clone ID Related Accession (Same CDS sequence) XM_011250419.2 , XM_011250419.3
Accession Version XM_011250419.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 642bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product calpain small subunit 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250419.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
ATGGAGGGAA AGGAGGGGTC AGACCATTCT CTTGATTGGA CCAGCGAGGC TGCTGCGCAG 
TACAACCCGG AGCCTCCGCC TCCACGTAGT CATTACTCCA ATATTGAGGC CAACGAGAGT 
GAAGAAGTCC GTCAGTTCAG GAAACTTTTT GTCCAGCTGG CTGGAGACGA CATGGAGGTC 
AGCGCCACAG AACTCATGAA TATCCTCAAC AAGGTCGTGA CCCGACACCC GGATCTGAAA 
ACTGATGGTT TTGGCATTGA CACTTGTCGG AGCATGGTGG CCGTCATGGA TAGCGACACC 
ACAGGCAAGC TGGGCTTTGA GGAGTTCAAA TACCTCTGGA ACAACATTAA AAAGTGGCAG 
GCTATATACA AACGCTTTGA TACTGACCGA TCAGGGACTA TCGGTAGCCA TGAACTTCCA 
GGGGCCTTTG AGGCAGCAGG ATTCCACCTG AATGAGCATC TCTACAGCAT GATCATCCGG 
CGCTACGCAG ATGAGAGCGG GAACATGGAT TTCGATAACT TCATCAGCTG CTTGGTCAGG 
CTAGATGCCA TGTTCCGCGC TTTCAAATCT CTTGACAAAA ATGGCACCGG ACAGATCCAA 
GTGAACATCC AGGAGTGGCT GCAGCTGACT ATGTATTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011248721.1
CDS114..755
Translation

Target ORF information:

RefSeq Version XM_011250419.3
Organism Mus musculus(house mouse)
Definition Mus musculus calpain, small subunit 1 (Capns1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011250419.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGAGGGAA AGGAGGGGTC AGACCATTCT CTTGATTGGA CCAGCGAGGC TGCTGCGCAG 
TACAACCCGG AGCCTCCGCC TCCACGTAGT CATTACTCCA ATATTGAGGC CAACGAGAGT 
GAAGAAGTCC GTCAGTTCAG GAAACTTTTT GTCCAGCTGG CTGGAGACGA CATGGAGGTC 
AGCGCCACAG AACTCATGAA TATCCTCAAC AAGGTCGTGA CCCGACACCC GGATCTGAAA 
ACTGATGGTT TTGGCATTGA CACTTGTCGG AGCATGGTGG CCGTCATGGA TAGCGACACC 
ACAGGCAAGC TGGGCTTTGA GGAGTTCAAA TACCTCTGGA ACAACATTAA AAAGTGGCAG 
GCTATATACA AACGCTTTGA TACTGACCGA TCAGGGACTA TCGGTAGCCA TGAACTTCCA 
GGGGCCTTTG AGGCAGCAGG ATTCCACCTG AATGAGCATC TCTACAGCAT GATCATCCGG 
CGCTACGCAG ATGAGAGCGG GAACATGGAT TTCGATAACT TCATCAGCTG CTTGGTCAGG 
CTAGATGCCA TGTTCCGCGC TTTCAAATCT CTTGACAAAA ATGGCACCGG ACAGATCCAA 
GTGAACATCC AGGAGTGGCT GCAGCTGACT ATGTATTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Disruption of calpain reduces lipotoxicity-induced cardiac injury by preventing endoplasmic reticulum stress.
Biochimica et biophysica acta1862(11)2023-2033(2016 Nov)
Li S,Zhang L,Ni R,Cao T,Zheng D,Xiong S,Greer PA,Fan GC,Peng T


book

Calpain Genetic Disruption and HSP90 Inhibition Combine To Attenuate Mammary Tumorigenesis.
Molecular and cellular biology36(15)2078-88(2016 Aug)
Grieve S,Gao Y,Hall C,Hu J,Greer PA


book

Calpain-2 activates Akt via TGF-?1-mTORC2 pathway in pulmonary artery smooth muscle cells.
American journal of physiology. Cell physiology311(1)C24-34(2016 Jul)
Abeyrathna P,Kovacs L,Han W,Su Y


book

Activation of Calpain-2 by Mediators in Pulmonary Vascular Remodeling of Pulmonary Arterial Hypertension.
American journal of respiratory cell and molecular biology54(3)384-93(2016 Mar)
Kovacs L,Han W,Rafikov R,Bagi Z,Offermanns S,Saido TC,Black SM,Su Y


book

Mitochondrial Calpain-1 Disrupts ATP Synthase and Induces Superoxide Generation in Type 1 Diabetic Hearts: A Novel Mechanism Contributing to Diabetic Cardiomyopathy.
Diabetes65(1)255-68(2016 Jan)
Ni R,Zheng D,Xiong S,Hill DJ,Sun T,Gardiner RB,Fan GC,Lu Y,Abel ED,Greer PA,Peng T