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Chrm4 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Chrm4
Entrez Gene ID 12672
Full Name cholinergic receptor, muscarinic 4
Synonyms Chrm-4,M4
General protein information
Preferred Names

cholinergic receptor, muscarinic 4

Names

muscarinic acetylcholine receptor M4
AChR M4
mm4 mAChR
muscarinic acetylcholine receptor 4
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

2

2 E1|2 50.63 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011239271.2 XP_011237573.1 muscarinic acetylcholine receptor M4 isoform X1
XM_006498654.2 XP_006498717.1 muscarinic acetylcholine receptor M4 isoform X2
NM_007699.2 NP_031725.1 muscarinic acetylcholine receptor M4
XM_006498654.3 XP_006498717.1 muscarinic acetylcholine receptor M4 isoform X1

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) CHRM4 XP_005631350.1
Rattus norvegicus (Norway rat) Chrm4 NP_113735.1
Gallus gallus (chicken) CHRM4 NP_001026362.1
Homo sapiens (human) CHRM4 NP_000732.2
Mus musculus (house mouse) Chrm4 NP_031725.1
Danio rerio (zebrafish) chrm4a XP_001922442.1
CHRM4 XP_001112370.1
Xenopus tropicalis (tropical clawed frog) chrm4 NP_001106514.1

Gene Ontology


Bibliography

Related articles in PubMed

Cholinergic Projections to the Substantia Nigra Pars Reticulata Inhibit Dopamine Modulation of Basal Ganglia through the M
Moehle MS, Pancani T, Byun N, Yohn SE, Wilson GH 3rd, Dickerson JW, Remke DH, Xiang Z, Niswender CM, Wess J, Jones CK, Lindsley CW, Rook JM, Conn PJ
Neuron96(6)1358-1372.e4(2017 Dec)

Effects of muscarinic receptor antagonists on cocaine discrimination in wild-type mice and in muscarinic receptor M
Joseph L, Thomsen M
Behavioural brain research32975-83(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the present study, the functional coupling of Gi/o protein-coupled receptors to GalR1, and the CB1 receptor subtype for endocannabinoids were analyzed in the 3xTg-AD mice model of Alzheimer's disease in the prodromal and advanced stages. In addition, the activity mediated by Gi/o protein-coupled M2/4 muscarinic receptor subtypes was also analyzed in brain areas involved in anxiety and cognition.
Title: Activity of muscarinic, galanin and cannabinoid receptors in the prodromal and advanced stages in the triple transgenic mice model of Alzheimer's disease.

The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings.
Title: Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction.

Allosteric activation of M4 muscarinic receptors improve behavioral and physiological alterations in early symptomatic Huntington's disease - YAC128 mice.
Title: Allosteric activation of M4 muscarinic receptors improve behavioral and physiological alterations in early symptomatic YAC128 mice.

Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin
Title: Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin.

the role of the M4 receptor in alcohol consumption
Title: Enhanced self-administration of alcohol in muscarinic acetylcholine M4 receptor knockout mice.

The following Chrm4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrm4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu00633 NM_007699.2
Latest version!		 Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $286.30
$409.00
OMu29963 XM_006498654.3
Latest version!		 Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OMu67368 XM_011239271.2
Latest version!		 Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $307.30
$439.00
View Old Accession Versions >>
OMu29963 XM_006498654.2 Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu29963
Clone ID Related Accession (Same CDS sequence) XM_006498654.2 , XM_006498654.3
Accession Version XM_006498654.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product muscarinic acetylcholine receptor M4 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006498654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGCCGG GAGATGGGCC CAAGGCCACT GCCCACCTGT CTCCAGTTGG GTTCAGCATG 
GCGAACTTCA CACCTGTCAA TGGCAGCTCA GCCAATCAGT CTGTGCGCCT GGTCACAACA 
GCCCACAACC ACCTGGAGAC AGTGGAGATG GTGTTCATTG CGACAGTGAC TGGTTCCCTG 
AGCCTGGTGA CTGTGGTGGG TAACATCCTG GTGATGCTGT CCATCAAGGT CAACAGGCAG 
TTGCAGACAG TCAACAACTA CTTCCTGTTC AGCCTGGCGT GTGCAGATCT CATCATAGGG 
GCGTTCTCTA TGAACCTTTA CACCTTATAC ATCATCAAGG GCTACTGGCC CCTGGGTGCC 
GTGGTCTGTG ACCTGTGGCT GGCCCTGGAC TATGTGGTGA GCAATGCCTC TGTCATGAAC 
CTTCTCATCA TCAGCTTTGA CCGCTATTTC TGCGTCACCA AGCCCCTCAC CTATCCAGCC 
CGCCGCACTA CTAAGATGGC AGGCCTCATG ATTGCAGCCG CCTGGGTCTT GTCCTTTGTA 
CTCTGGGCCC CTGCCATCTT GTTCTGGCAG TTTGTGGTGG GCAAGAGGAC AGTGCCTGAT 
AACCAGTGCT TCATCCAGTT CTTGTCCAAC CCGGCGGTGA CCTTCGGCAC AGCCATTGCT 
GCCTTCTACC TGCCTGTGGT CATCATGACG GTGCTGTATA TTCATATCTC GCTGGCCAGC 
CGCAGCCGTG TTCACAAGCA TCGACCCGAG GGCCCCAAGG AGAAGAAGGC CAAGACTCTG 
GCTTTCCTCA AGAGCCCTCT GATGAAGCCG AGCATTAAGA AACCTCCACC AGGGGGCGCT 
TCTCGAGAGG AACTGCGCAA CGGGAAGCTA GAAGAGGCTC CTCCGCCAGC CCTGCCCCCG 
CCTCCACGCC CAGTGGCTGA CAAGGACACT TCCAATGAGT CCAGCTCAGG CAGTGCCACC 
CAGAACACCA AGGAACGGCC ACCCACAGAG CTGTCCACCA CAGAGGCCGC CACCACACCA 
GCGCTGCCCG CTCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA GTGGTCCAAG 
ATCCAAATTG TGACAAAGCA GACAGGCAGT GAATGTGTGA CTGCCATCGA GATCGTACCT 
GCCACGCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATCGCT 
CGTAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAAAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACGAT CAACCCTGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006498717.1
CDS892..2388
Translation

Target ORF information:

RefSeq Version XM_006498654.2
Organism Mus musculus(house mouse)
Definition Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006498654.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGCCGG GAGATGGGCC CAAGGCCACT GCCCACCTGT CTCCAGTTGG GTTCAGCATG 
GCGAACTTCA CACCTGTCAA TGGCAGCTCA GCCAATCAGT CTGTGCGCCT GGTCACAACA 
GCCCACAACC ACCTGGAGAC AGTGGAGATG GTGTTCATTG CGACAGTGAC TGGTTCCCTG 
AGCCTGGTGA CTGTGGTGGG TAACATCCTG GTGATGCTGT CCATCAAGGT CAACAGGCAG 
TTGCAGACAG TCAACAACTA CTTCCTGTTC AGCCTGGCGT GTGCAGATCT CATCATAGGG 
GCGTTCTCTA TGAACCTTTA CACCTTATAC ATCATCAAGG GCTACTGGCC CCTGGGTGCC 
GTGGTCTGTG ACCTGTGGCT GGCCCTGGAC TATGTGGTGA GCAATGCCTC TGTCATGAAC 
CTTCTCATCA TCAGCTTTGA CCGCTATTTC TGCGTCACCA AGCCCCTCAC CTATCCAGCC 
CGCCGCACTA CTAAGATGGC AGGCCTCATG ATTGCAGCCG CCTGGGTCTT GTCCTTTGTA 
CTCTGGGCCC CTGCCATCTT GTTCTGGCAG TTTGTGGTGG GCAAGAGGAC AGTGCCTGAT 
AACCAGTGCT TCATCCAGTT CTTGTCCAAC CCGGCGGTGA CCTTCGGCAC AGCCATTGCT 
GCCTTCTACC TGCCTGTGGT CATCATGACG GTGCTGTATA TTCATATCTC GCTGGCCAGC 
CGCAGCCGTG TTCACAAGCA TCGACCCGAG GGCCCCAAGG AGAAGAAGGC CAAGACTCTG 
GCTTTCCTCA AGAGCCCTCT GATGAAGCCG AGCATTAAGA AACCTCCACC AGGGGGCGCT 
TCTCGAGAGG AACTGCGCAA CGGGAAGCTA GAAGAGGCTC CTCCGCCAGC CCTGCCCCCG 
CCTCCACGCC CAGTGGCTGA CAAGGACACT TCCAATGAGT CCAGCTCAGG CAGTGCCACC 
CAGAACACCA AGGAACGGCC ACCCACAGAG CTGTCCACCA CAGAGGCCGC CACCACACCA 
GCGCTGCCCG CTCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA GTGGTCCAAG 
ATCCAAATTG TGACAAAGCA GACAGGCAGT GAATGTGTGA CTGCCATCGA GATCGTACCT 
GCCACGCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATCGCT 
CGTAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAAAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACGAT CAACCCTGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu00633
Clone ID Related Accession (Same CDS sequence) NM_007699.2
Accession Version NM_007699.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1440bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-26
Organism Mus musculus(house mouse)
Product muscarinic acetylcholine receptor M4
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY250825.1, AL731772.9, BC120504.1, CX122754.1, CX241098.1 and BY652985.1. On Oct 12, 2007 this sequence version replaced NM_007699.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY250825.1, CJ145250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGAACT TCACACCTGT CAATGGCAGC TCAGCCAATC AGTCTGTGCG CCTGGTCACA 
ACAGCCCACA ACCACCTGGA GACAGTGGAG ATGGTGTTCA TTGCGACAGT GACTGGTTCC 
CTGAGCCTGG TGACTGTGGT GGGTAACATC CTGGTGATGC TGTCCATCAA GGTCAACAGG 
CAGTTGCAGA CAGTCAACAA CTACTTCCTG TTCAGCCTGG CGTGTGCAGA TCTCATCATA 
GGGGCGTTCT CTATGAACCT TTACACCTTA TACATCATCA AGGGCTACTG GCCCCTGGGT 
GCCGTGGTCT GTGACCTGTG GCTGGCCCTG GACTATGTGG TGAGCAATGC CTCTGTCATG 
AACCTTCTCA TCATCAGCTT TGACCGCTAT TTCTGCGTCA CCAAGCCCCT CACCTATCCA 
GCCCGCCGCA CTACTAAGAT GGCAGGCCTC ATGATTGCAG CCGCCTGGGT CTTGTCCTTT 
GTACTCTGGG CCCCTGCCAT CTTGTTCTGG CAGTTTGTGG TGGGCAAGAG GACAGTGCCT 
GATAACCAGT GCTTCATCCA GTTCTTGTCC AACCCGGCGG TGACCTTCGG CACAGCCATT 
GCTGCCTTCT ACCTGCCTGT GGTCATCATG ACGGTGCTGT ATATTCATAT CTCGCTGGCC 
AGCCGCAGCC GTGTTCACAA GCATCGACCC GAGGGCCCCA AGGAGAAGAA GGCCAAGACT 
CTGGCTTTCC TCAAGAGCCC TCTGATGAAG CCGAGCATTA AGAAACCTCC ACCAGGGGGC 
GCTTCTCGAG AGGAACTGCG CAACGGGAAG CTAGAAGAGG CTCCTCCGCC AGCCCTGCCC 
CCGCCTCCAC GCCCAGTGGC TGACAAGGAC ACTTCCAATG AGTCCAGCTC AGGCAGTGCC 
ACCCAGAACA CCAAGGAACG GCCACCCACA GAGCTGTCCA CCACAGAGGC CGCCACCACA 
CCAGCGCTGC CCGCTCCTAC CCTGCAGCCA CGAACCCTCA ACCCAGCCTC CAAGTGGTCC 
AAGATCCAAA TTGTGACAAA GCAGACAGGC AGTGAATGTG TGACTGCCAT CGAGATCGTA 
CCTGCCACGC CAGCTGGTAT GCGCCCAGCA GCCAATGTGG CCCGAAAGTT TGCCAGCATC 
GCTCGTAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACTCGG 
ACAATCTTTG CCATTCTGCT GGCCTTCATC CTCACCTGGA CACCCTACAA TGTCATGGTC 
CTGGTGAACA CCTTTTGCCA GAGCTGTATC CCCGAAAGGG TGTGGTCCAT CGGCTACTGG 
CTCTGCTACG TCAACAGCAC GATCAACCCT GCCTGCTATG CACTCTGCAA TGCCACTTTC 
AAAAAGACCT TCCGGCACCT TTTGCTGTGC CAGTATCGGA ACATCGGCAC AGCCAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_031725.1
CDS188..1627
Translation

Target ORF information:

RefSeq Version NM_007699.2
Organism Mus musculus(house mouse)
Definition Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_007699.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCGAACT TCACACCTGT CAATGGCAGC TCAGCCAATC AGTCTGTGCG CCTGGTCACA 
ACAGCCCACA ACCACCTGGA GACAGTGGAG ATGGTGTTCA TTGCGACAGT GACTGGTTCC 
CTGAGCCTGG TGACTGTGGT GGGTAACATC CTGGTGATGC TGTCCATCAA GGTCAACAGG 
CAGTTGCAGA CAGTCAACAA CTACTTCCTG TTCAGCCTGG CGTGTGCAGA TCTCATCATA 
GGGGCGTTCT CTATGAACCT TTACACCTTA TACATCATCA AGGGCTACTG GCCCCTGGGT 
GCCGTGGTCT GTGACCTGTG GCTGGCCCTG GACTATGTGG TGAGCAATGC CTCTGTCATG 
AACCTTCTCA TCATCAGCTT TGACCGCTAT TTCTGCGTCA CCAAGCCCCT CACCTATCCA 
GCCCGCCGCA CTACTAAGAT GGCAGGCCTC ATGATTGCAG CCGCCTGGGT CTTGTCCTTT 
GTACTCTGGG CCCCTGCCAT CTTGTTCTGG CAGTTTGTGG TGGGCAAGAG GACAGTGCCT 
GATAACCAGT GCTTCATCCA GTTCTTGTCC AACCCGGCGG TGACCTTCGG CACAGCCATT 
GCTGCCTTCT ACCTGCCTGT GGTCATCATG ACGGTGCTGT ATATTCATAT CTCGCTGGCC 
AGCCGCAGCC GTGTTCACAA GCATCGACCC GAGGGCCCCA AGGAGAAGAA GGCCAAGACT 
CTGGCTTTCC TCAAGAGCCC TCTGATGAAG CCGAGCATTA AGAAACCTCC ACCAGGGGGC 
GCTTCTCGAG AGGAACTGCG CAACGGGAAG CTAGAAGAGG CTCCTCCGCC AGCCCTGCCC 
CCGCCTCCAC GCCCAGTGGC TGACAAGGAC ACTTCCAATG AGTCCAGCTC AGGCAGTGCC 
ACCCAGAACA CCAAGGAACG GCCACCCACA GAGCTGTCCA CCACAGAGGC CGCCACCACA 
CCAGCGCTGC CCGCTCCTAC CCTGCAGCCA CGAACCCTCA ACCCAGCCTC CAAGTGGTCC 
AAGATCCAAA TTGTGACAAA GCAGACAGGC AGTGAATGTG TGACTGCCAT CGAGATCGTA 
CCTGCCACGC CAGCTGGTAT GCGCCCAGCA GCCAATGTGG CCCGAAAGTT TGCCAGCATC 
GCTCGTAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACTCGG 
ACAATCTTTG CCATTCTGCT GGCCTTCATC CTCACCTGGA CACCCTACAA TGTCATGGTC 
CTGGTGAACA CCTTTTGCCA GAGCTGTATC CCCGAAAGGG TGTGGTCCAT CGGCTACTGG 
CTCTGCTACG TCAACAGCAC GATCAACCCT GCCTGCTATG CACTCTGCAA TGCCACTTTC 
AAAAAGACCT TCCGGCACCT TTTGCTGTGC CAGTATCGGA ACATCGGCAC AGCCAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu29963
Clone ID Related Accession (Same CDS sequence) XM_006498654.2 , XM_006498654.3
Accession Version XM_006498654.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product muscarinic acetylcholine receptor M4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGCCGG GAGATGGGCC CAAGGCCACT GCCCACCTGT CTCCAGTTGG GTTCAGCATG 
GCGAACTTCA CACCTGTCAA TGGCAGCTCA GCCAATCAGT CTGTGCGCCT GGTCACAACA 
GCCCACAACC ACCTGGAGAC AGTGGAGATG GTGTTCATTG CGACAGTGAC TGGTTCCCTG 
AGCCTGGTGA CTGTGGTGGG TAACATCCTG GTGATGCTGT CCATCAAGGT CAACAGGCAG 
TTGCAGACAG TCAACAACTA CTTCCTGTTC AGCCTGGCGT GTGCAGATCT CATCATAGGG 
GCGTTCTCTA TGAACCTTTA CACCTTATAC ATCATCAAGG GCTACTGGCC CCTGGGTGCC 
GTGGTCTGTG ACCTGTGGCT GGCCCTGGAC TATGTGGTGA GCAATGCCTC TGTCATGAAC 
CTTCTCATCA TCAGCTTTGA CCGCTATTTC TGCGTCACCA AGCCCCTCAC CTATCCAGCC 
CGCCGCACTA CTAAGATGGC AGGCCTCATG ATTGCAGCCG CCTGGGTCTT GTCCTTTGTA 
CTCTGGGCCC CTGCCATCTT GTTCTGGCAG TTTGTGGTGG GCAAGAGGAC AGTGCCTGAT 
AACCAGTGCT TCATCCAGTT CTTGTCCAAC CCGGCGGTGA CCTTCGGCAC AGCCATTGCT 
GCCTTCTACC TGCCTGTGGT CATCATGACG GTGCTGTATA TTCATATCTC GCTGGCCAGC 
CGCAGCCGTG TTCACAAGCA TCGACCCGAG GGCCCCAAGG AGAAGAAGGC CAAGACTCTG 
GCTTTCCTCA AGAGCCCTCT GATGAAGCCG AGCATTAAGA AACCTCCACC AGGGGGCGCT 
TCTCGAGAGG AACTGCGCAA CGGGAAGCTA GAAGAGGCTC CTCCGCCAGC CCTGCCCCCG 
CCTCCACGCC CAGTGGCTGA CAAGGACACT TCCAATGAGT CCAGCTCAGG CAGTGCCACC 
CAGAACACCA AGGAACGGCC ACCCACAGAG CTGTCCACCA CAGAGGCCGC CACCACACCA 
GCGCTGCCCG CTCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA GTGGTCCAAG 
ATCCAAATTG TGACAAAGCA GACAGGCAGT GAATGTGTGA CTGCCATCGA GATCGTACCT 
GCCACGCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATCGCT 
CGTAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAAAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACGAT CAACCCTGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006498717.1
CDS891..2387
Translation

Target ORF information:

RefSeq Version XM_006498654.3
Organism Mus musculus(house mouse)
Definition Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006498654.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGAGCCGG GAGATGGGCC CAAGGCCACT GCCCACCTGT CTCCAGTTGG GTTCAGCATG 
GCGAACTTCA CACCTGTCAA TGGCAGCTCA GCCAATCAGT CTGTGCGCCT GGTCACAACA 
GCCCACAACC ACCTGGAGAC AGTGGAGATG GTGTTCATTG CGACAGTGAC TGGTTCCCTG 
AGCCTGGTGA CTGTGGTGGG TAACATCCTG GTGATGCTGT CCATCAAGGT CAACAGGCAG 
TTGCAGACAG TCAACAACTA CTTCCTGTTC AGCCTGGCGT GTGCAGATCT CATCATAGGG 
GCGTTCTCTA TGAACCTTTA CACCTTATAC ATCATCAAGG GCTACTGGCC CCTGGGTGCC 
GTGGTCTGTG ACCTGTGGCT GGCCCTGGAC TATGTGGTGA GCAATGCCTC TGTCATGAAC 
CTTCTCATCA TCAGCTTTGA CCGCTATTTC TGCGTCACCA AGCCCCTCAC CTATCCAGCC 
CGCCGCACTA CTAAGATGGC AGGCCTCATG ATTGCAGCCG CCTGGGTCTT GTCCTTTGTA 
CTCTGGGCCC CTGCCATCTT GTTCTGGCAG TTTGTGGTGG GCAAGAGGAC AGTGCCTGAT 
AACCAGTGCT TCATCCAGTT CTTGTCCAAC CCGGCGGTGA CCTTCGGCAC AGCCATTGCT 
GCCTTCTACC TGCCTGTGGT CATCATGACG GTGCTGTATA TTCATATCTC GCTGGCCAGC 
CGCAGCCGTG TTCACAAGCA TCGACCCGAG GGCCCCAAGG AGAAGAAGGC CAAGACTCTG 
GCTTTCCTCA AGAGCCCTCT GATGAAGCCG AGCATTAAGA AACCTCCACC AGGGGGCGCT 
TCTCGAGAGG AACTGCGCAA CGGGAAGCTA GAAGAGGCTC CTCCGCCAGC CCTGCCCCCG 
CCTCCACGCC CAGTGGCTGA CAAGGACACT TCCAATGAGT CCAGCTCAGG CAGTGCCACC 
CAGAACACCA AGGAACGGCC ACCCACAGAG CTGTCCACCA CAGAGGCCGC CACCACACCA 
GCGCTGCCCG CTCCTACCCT GCAGCCACGA ACCCTCAACC CAGCCTCCAA GTGGTCCAAG 
ATCCAAATTG TGACAAAGCA GACAGGCAGT GAATGTGTGA CTGCCATCGA GATCGTACCT 
GCCACGCCAG CTGGTATGCG CCCAGCAGCC AATGTGGCCC GAAAGTTTGC CAGCATCGCT 
CGTAACCAGG TGCGCAAGAA GCGGCAGATG GCGGCCCGGG AGCGCAAAGT GACTCGGACA 
ATCTTTGCCA TTCTGCTGGC CTTCATCCTC ACCTGGACAC CCTACAATGT CATGGTCCTG 
GTGAACACCT TTTGCCAGAG CTGTATCCCC GAAAGGGTGT GGTCCATCGG CTACTGGCTC 
TGCTACGTCA ACAGCACGAT CAACCCTGCC TGCTATGCAC TCTGCAATGC CACTTTCAAA 
AAGACCTTCC GGCACCTTTT GCTGTGCCAG TATCGGAACA TCGGCACAGC CAGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu67368
Clone ID Related Accession (Same CDS sequence) XM_011239271.2
Accession Version XM_011239271.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product muscarinic acetylcholine receptor M4 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGACAACGC GGACAGCCCC CACCCCACCC CCCGGAGGGC AGAGCCGGAG GCTAATCCTC 
CCTGGCGACT GTGGGCGGTG CCCTCGGTGC TGGAGTCCGG AGTTGCGGGC CATCTCCCGG 
GAAGGGTGTG AGCATCAAGG GTGCCTGACA CTCCAGGTGG TGACCCCCGC GCGTCGCCGC 
TGGGTGACCG GCCCCGGGCG GGCGGTGCGT CCCCACCACG ATTGGCTGCT TCCCTGTGAC 
ATCACGCGGC TCGGAGAAAA GTGCGAGCGT GAGCGCGAGT CGGAGCCACA GCGCCGGGAG 
CTGCAGGCGA AGCAGGGGCC GCCCCTTGGC ACCCTGTCCC CGCCCCCGGC CCCCGGCCCA 
GTCGCCCACC CCACCGACTC GCAGGGCCTC TGGCTAGTTC CGCCGTCTGT CCGGCGCCAG 
CGGAGCAAGA CAGAAGCAGC GGCCACTGCC CACCTGTCTC CAGTTGGGTT CAGCATGGCG 
AACTTCACAC CTGTCAATGG CAGCTCAGCC AATCAGTCTG TGCGCCTGGT CACAACAGCC 
CACAACCACC TGGAGACAGT GGAGATGGTG TTCATTGCGA CAGTGACTGG TTCCCTGAGC 
CTGGTGACTG TGGTGGGTAA CATCCTGGTG ATGCTGTCCA TCAAGGTCAA CAGGCAGTTG 
CAGACAGTCA ACAACTACTT CCTGTTCAGC CTGGCGTGTG CAGATCTCAT CATAGGGGCG 
TTCTCTATGA ACCTTTACAC CTTATACATC ATCAAGGGCT ACTGGCCCCT GGGTGCCGTG 
GTCTGTGACC TGTGGCTGGC CCTGGACTAT GTGGTGAGCA ATGCCTCTGT CATGAACCTT 
CTCATCATCA GCTTTGACCG CTATTTCTGC GTCACCAAGC CCCTCACCTA TCCAGCCCGC 
CGCACTACTA AGATGGCAGG CCTCATGATT GCAGCCGCCT GGGTCTTGTC CTTTGTACTC 
TGGGCCCCTG CCATCTTGTT CTGGCAGTTT GTGGTGGGCA AGAGGACAGT GCCTGATAAC 
CAGTGCTTCA TCCAGTTCTT GTCCAACCCG GCGGTGACCT TCGGCACAGC CATTGCTGCC 
TTCTACCTGC CTGTGGTCAT CATGACGGTG CTGTATATTC ATATCTCGCT GGCCAGCCGC 
AGCCGTGTTC ACAAGCATCG ACCCGAGGGC CCCAAGGAGA AGAAGGCCAA GACTCTGGCT 
TTCCTCAAGA GCCCTCTGAT GAAGCCGAGC ATTAAGAAAC CTCCACCAGG GGGCGCTTCT 
CGAGAGGAAC TGCGCAACGG GAAGCTAGAA GAGGCTCCTC CGCCAGCCCT GCCCCCGCCT 
CCACGCCCAG TGGCTGACAA GGACACTTCC AATGAGTCCA GCTCAGGCAG TGCCACCCAG 
AACACCAAGG AACGGCCACC CACAGAGCTG TCCACCACAG AGGCCGCCAC CACACCAGCG 
CTGCCCGCTC CTACCCTGCA GCCACGAACC CTCAACCCAG CCTCCAAGTG GTCCAAGATC 
CAAATTGTGA CAAAGCAGAC AGGCAGTGAA TGTGTGACTG CCATCGAGAT CGTACCTGCC 
ACGCCAGCTG GTATGCGCCC AGCAGCCAAT GTGGCCCGAA AGTTTGCCAG CATCGCTCGT 
AACCAGGTGC GCAAGAAGCG GCAGATGGCG GCCCGGGAGC GCAAAGTGAC TCGGACAATC 
TTTGCCATTC TGCTGGCCTT CATCCTCACC TGGACACCCT ACAATGTCAT GGTCCTGGTG 
AACACCTTTT GCCAGAGCTG TATCCCCGAA AGGGTGTGGT CCATCGGCTA CTGGCTCTGC 
TACGTCAACA GCACGATCAA CCCTGCCTGC TATGCACTCT GCAATGCCAC TTTCAAAAAG 
ACCTTCCGGC ACCTTTTGCT GTGCCAGTAT CGGAACATCG GCACAGCCAG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011237573.1
CDS206..2119
Translation

Target ORF information:

RefSeq Version XM_011239271.2
Organism Mus musculus(house mouse)
Definition Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011239271.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
ATGACAACGC GGACAGCCCC CACCCCACCC CCCGGAGGGC AGAGCCGGAG GCTAATCCTC 
CCTGGCGACT GTGGGCGGTG CCCTCGGTGC TGGAGTCCGG AGTTGCGGGC CATCTCCCGG 
GAAGGGTGTG AGCATCAAGG GTGCCTGACA CTCCAGGTGG TGACCCCCGC GCGTCGCCGC 
TGGGTGACCG GCCCCGGGCG GGCGGTGCGT CCCCACCACG ATTGGCTGCT TCCCTGTGAC 
ATCACGCGGC TCGGAGAAAA GTGCGAGCGT GAGCGCGAGT CGGAGCCACA GCGCCGGGAG 
CTGCAGGCGA AGCAGGGGCC GCCCCTTGGC ACCCTGTCCC CGCCCCCGGC CCCCGGCCCA 
GTCGCCCACC CCACCGACTC GCAGGGCCTC TGGCTAGTTC CGCCGTCTGT CCGGCGCCAG 
CGGAGCAAGA CAGAAGCAGC GGCCACTGCC CACCTGTCTC CAGTTGGGTT CAGCATGGCG 
AACTTCACAC CTGTCAATGG CAGCTCAGCC AATCAGTCTG TGCGCCTGGT CACAACAGCC 
CACAACCACC TGGAGACAGT GGAGATGGTG TTCATTGCGA CAGTGACTGG TTCCCTGAGC 
CTGGTGACTG TGGTGGGTAA CATCCTGGTG ATGCTGTCCA TCAAGGTCAA CAGGCAGTTG 
CAGACAGTCA ACAACTACTT CCTGTTCAGC CTGGCGTGTG CAGATCTCAT CATAGGGGCG 
TTCTCTATGA ACCTTTACAC CTTATACATC ATCAAGGGCT ACTGGCCCCT GGGTGCCGTG 
GTCTGTGACC TGTGGCTGGC CCTGGACTAT GTGGTGAGCA ATGCCTCTGT CATGAACCTT 
CTCATCATCA GCTTTGACCG CTATTTCTGC GTCACCAAGC CCCTCACCTA TCCAGCCCGC 
CGCACTACTA AGATGGCAGG CCTCATGATT GCAGCCGCCT GGGTCTTGTC CTTTGTACTC 
TGGGCCCCTG CCATCTTGTT CTGGCAGTTT GTGGTGGGCA AGAGGACAGT GCCTGATAAC 
CAGTGCTTCA TCCAGTTCTT GTCCAACCCG GCGGTGACCT TCGGCACAGC CATTGCTGCC 
TTCTACCTGC CTGTGGTCAT CATGACGGTG CTGTATATTC ATATCTCGCT GGCCAGCCGC 
AGCCGTGTTC ACAAGCATCG ACCCGAGGGC CCCAAGGAGA AGAAGGCCAA GACTCTGGCT 
TTCCTCAAGA GCCCTCTGAT GAAGCCGAGC ATTAAGAAAC CTCCACCAGG GGGCGCTTCT 
CGAGAGGAAC TGCGCAACGG GAAGCTAGAA GAGGCTCCTC CGCCAGCCCT GCCCCCGCCT 
CCACGCCCAG TGGCTGACAA GGACACTTCC AATGAGTCCA GCTCAGGCAG TGCCACCCAG 
AACACCAAGG AACGGCCACC CACAGAGCTG TCCACCACAG AGGCCGCCAC CACACCAGCG 
CTGCCCGCTC CTACCCTGCA GCCACGAACC CTCAACCCAG CCTCCAAGTG GTCCAAGATC 
CAAATTGTGA CAAAGCAGAC AGGCAGTGAA TGTGTGACTG CCATCGAGAT CGTACCTGCC 
ACGCCAGCTG GTATGCGCCC AGCAGCCAAT GTGGCCCGAA AGTTTGCCAG CATCGCTCGT 
AACCAGGTGC GCAAGAAGCG GCAGATGGCG GCCCGGGAGC GCAAAGTGAC TCGGACAATC 
TTTGCCATTC TGCTGGCCTT CATCCTCACC TGGACACCCT ACAATGTCAT GGTCCTGGTG 
AACACCTTTT GCCAGAGCTG TATCCCCGAA AGGGTGTGGT CCATCGGCTA CTGGCTCTGC 
TACGTCAACA GCACGATCAA CCCTGCCTGC TATGCACTCT GCAATGCCAC TTTCAAAAAG 
ACCTTCCGGC ACCTTTTGCT GTGCCAGTAT CGGAACATCG GCACAGCCAG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Cholinergic Projections to the Substantia Nigra Pars Reticulata Inhibit Dopamine Modulation of Basal Ganglia through the M
Neuron96(6)1358-1372.e4(2017 Dec)
Moehle MS,Pancani T,Byun N,Yohn SE,Wilson GH 3rd,Dickerson JW,Remke DH,Xiang Z,Niswender CM,Wess J,Jones CK,Lindsley CW,Rook JM,Conn PJ


book

Effects of muscarinic receptor antagonists on cocaine discrimination in wild-type mice and in muscarinic receptor M
Behavioural brain research32975-83(2017 Jun)
Joseph L,Thomsen M