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Cyp46a1 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Cyp46a1
Entrez Gene ID 13116
Full Name cytochrome P450, family 46, subfamily a, polypeptide 1
Synonyms Cyp46
General protein information
Preferred Names

cytochrome P450, family 46, subfamily a, polypeptide 1

Names

cholesterol 24-hydroxylase
CH24H
cholestrol 24-hydroxylase
cytochrome P450 46A1
cytochrome P450, 46 (cholesterol 24-hydroxylase)
cytochrome P450, 46a1 (cholesterol 24-hydroxylase)
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

12

12|12 F1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_010010.2 NP_034140.1 cholesterol 24-hydroxylase precursor
XM_030246535.1 XP_030102395.1 cholesterol 24-hydroxylase isoform X1
NM_010010.1 NP_034140.1 cholesterol 24-hydroxylase precursor
XM_006515456.3 XP_006515519.1 cholesterol 24-hydroxylase isoform X1

Pathways from BioSystems


Homologies


LOC705843 XP_001103455.2
Gallus gallus (chicken) CYP46A1 NP_001238956.1
Arabidopsis thaliana (thale cress) CYP97A3 NP_564384.1
Canis lupus familiaris (dog) CYP46A1 XP_005623990.1
Rattus norvegicus (Norway rat) Cyp46a1 NP_001102193.1
Magnaporthe oryzae (rice blast fungus) MGG_04469 XP_003710992.1
Xenopus tropicalis (tropical clawed frog) mgc108213 NP_001027517.1
Xenopus tropicalis (tropical clawed frog) mgc108077 NP_001027519.1
Mus musculus (house mouse) Cyp46a1 NP_034140.1
Xenopus tropicalis (tropical clawed frog) cyp46a1 NP_001072550.1
Homo sapiens (human) CYP46A1 NP_006659.1
Pan troglodytes (chimpanzee) CYP46A1 XP_510158.3
Bos taurus (cattle) CYP46A1 NP_001070278.1
Magnaporthe oryzae (rice blast fungus) MGG_14217 XP_003720504.1
Oryza sativa (rice) Os02g0817900 NP_001048526.1

Gene Ontology


Bibliography

Related articles in PubMed

Cholesterol-metabolizing enzyme cytochrome P450 46A1 as a pharmacologic target for Alzheimer's disease.
Mast N, Saadane A, Valencia-Olvera A, Constans J, Maxfield E, Arakawa H, Li Y, Landreth G, Pikuleva IA
Neuropharmacology123465-476(2017 Sep)

Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1).
Mast N, Lin JB, Anderson KW, Bjorkhem I, Pikuleva IA
PloS one12(10)e0187168(2017)

24S-hydroxycholesterol and 25-hydroxycholesterol differentially impact hippocampal neuronal survival following oxygen-glucose deprivation.
Sun MY, Taylor A, Zorumski CF, Mennerick S
PloS one12(3)e0174416(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study demonstrates the diverse potential effects of CYP46A1 deficiency on brain functions and identifies important proteins that could be affected by this deficiency
Title: Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1).

Increased expression of CYP46A1 in the striatum decreased neuronal atrophy in a Huntington's disease model.
Title: CYP46A1, the rate-limiting enzyme for cholesterol degradation, is neuroprotective in Huntington's disease.

under conditions of membrane cholesterol reduction by increased CYP46A1 expression, neurons increase isoprenoid synthesis and sGTPase prenylation. This leads to a reduction in liver X receptor activity
Title: Cholesterol 24S-Hydroxylase Overexpression Inhibits the Liver X Receptor (LXR) Pathway by Activating Small Guanosine Triphosphate-Binding Proteins (sGTPases) in Neuronal Cells.

EFV enhanced CYP46A1 activity and cerebral cholesterol turnover in animals with no effect on the levels of brain cholesterol.
Title: Pharmacologic stimulation of cytochrome P450 46A1 and cerebral cholesterol turnover in mice.

The expression of CYP46A1, as well as other cytochrome P450s involved in cholesterol homeostasis, are potently regulated by histone acetylation status.
Title: Transcriptional regulation of cholesterol 24-hydroxylase by histone deacetylase inhibitors.

The following Cyp46a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp46a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu17811 NM_010010.2
Latest version!		 Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OMu47568 XM_006515456.3
Latest version!		 Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu47568 XM_030246535.1
Latest version!		 Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OMu17811 NM_010010.1 Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu17811
Clone ID Related Accession (Same CDS sequence) NM_010010.2 , NM_010010.1
Accession Version NM_010010.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-26
Organism Mus musculus(house mouse)
Product cholesterol 24-hydroxylase precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY238426.1 and AF094479.1. On Apr 11, 2018 this sequence version replaced NM_010010.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018307.1, SRR1660813.62813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ATCGCGCTCG CAGCCGCTAT GAGCACATCC CCGGGCCGCC GCGGCCCAGC 
TTCCTTCTTG GACATCTCCC CTACTTTTGG AAAAAGGACG AAGATTGTGG CCGTGTGCTC 
CAAGATGTGT TTCTGGATTG GGCTAAGAAG TATGGTCCTG TTGTAAGAGT CAATGTCTTC 
TACAAGACCT CAGTCATTGT CACGAGTCCG GAGTCAGTCA AGAAGTTCCT GATGTCCACC 
AAGTACAACA AGGACTCCAA GATGTACCGC GCGCTTCAGA CTGTGTTTGG GGAGAGACTG 
TTTGGCCAGG GCTTGGTGTC TGAATGTGAC TATGGGCGCT GGTACAAGCA GAGGAAAGTC 
ATGGACTTGG CCTTCAGCCG CAGCTCCTTG GTCAGCCTGA TGGAGACGTT CAACGAGAAG 
GCGGAGCAGC TGGTGGAAAT CCTAGAAGCT AAGGCAGATG GACAGACACC CGTGTCCATG 
CAGGACATGC TGACCTGTGC CACCATCGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG 
ACCAGTATGT TACTGGGTGC CCAGAAACCG CTGTCCCAGG CAGTGAAGGT CATGCTGGAG 
GGCATCAGTG CATCCCGTAA CACCCTGGCA AAGTTCATGC CAGGGAAGAG AAAGCAGCTT 
CGGGAGATCC GCGAGAGCAT CCGTCTGCTG CGCCAGGTGG GGAAGGATTG GGTGCAGCGC 
CGCCGCGAAG CCCTGAAGAG GGGCGAGGAC ATGCCGGCTG ACATCCTCAC GCAGATCCTC 
AAAGCTGAAG AGGGAGCTCA GGACGATGAG GTTCTGCTGG ACAACTTTGT CACCTTCTTC 
ATTGCGGGTC ATGAGACTTC TGCCAACCAT CTGGCATTCA CAGTGATGGA GTTGTCTCGC 
CAGCCAGAGA TTGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTTGTCGG TTCCAAGAGG 
CACCTGGACT ACGAGGATCT GGGGAGACTG CAGTATCTGT CGCAGGTCCT CAAAGAGTCT 
CTGAGGCTGT ACCCGCCAGC GTGGGGCACC TTTCGCCTGC TGGAGGAGGA GACCTTGATT 
GATGGGGTCA GAGTCCCTGG CAATACTCCT CTCCTGTTCA GCACCTACGT CATGGGGAGA 
ATGGACACCT ACTTTGAAGA CCCATTGACT TTCAACCCTG ACCGCTTCGG CCCTGGAGCG 
CCCAAGCCAC GGTTCACTTA CTTTCCCTTT TCCCTGGGCC ACCGGTCCTG CATCGGCCAA 
CAGTTTGCTC AGATGGAGGT GAAAGTTGTC ATGGCCAAGC TGCTTCAGAG GATCGAGTTC 
CGGCTAGTGC CTGGGCAGCG CTTTGGGCTG CAAGAGCAGG CTACGCTCAA GCCATTGGAC 
CCCGTGCTGT GCACCCTGAG GCCCCGGGGC TGGCAGCCTG CACCCCCACC CCCACCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_034140.1
CDS131..1633
Translation

Target ORF information:

RefSeq Version NM_010010.2
Organism Mus musculus(house mouse)
Definition Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_010010.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ATCGCGCTCG CAGCCGCTAT GAGCACATCC CCGGGCCGCC GCGGCCCAGC 
TTCCTTCTTG GACATCTCCC CTACTTTTGG AAAAAGGACG AAGATTGTGG CCGTGTGCTC 
CAAGATGTGT TTCTGGATTG GGCTAAGAAG TATGGTCCTG TTGTAAGAGT CAATGTCTTC 
TACAAGACCT CAGTCATTGT CACGAGTCCG GAGTCAGTCA AGAAGTTCCT GATGTCCACC 
AAGTACAACA AGGACTCCAA GATGTACCGC GCGCTTCAGA CTGTGTTTGG GGAGAGACTG 
TTTGGCCAGG GCTTGGTGTC TGAATGTGAC TATGGGCGCT GGTACAAGCA GAGGAAAGTC 
ATGGACTTGG CCTTCAGCCG CAGCTCCTTG GTCAGCCTGA TGGAGACGTT CAACGAGAAG 
GCGGAGCAGC TGGTGGAAAT CCTAGAAGCT AAGGCAGATG GACAGACACC CGTGTCCATG 
CAGGACATGC TGACCTGTGC CACCATCGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG 
ACCAGTATGT TACTGGGTGC CCAGAAACCG CTGTCCCAGG CAGTGAAGGT CATGCTGGAG 
GGCATCAGTG CATCCCGTAA CACCCTGGCA AAGTTCATGC CAGGGAAGAG AAAGCAGCTT 
CGGGAGATCC GCGAGAGCAT CCGTCTGCTG CGCCAGGTGG GGAAGGATTG GGTGCAGCGC 
CGCCGCGAAG CCCTGAAGAG GGGCGAGGAC ATGCCGGCTG ACATCCTCAC GCAGATCCTC 
AAAGCTGAAG AGGGAGCTCA GGACGATGAG GTTCTGCTGG ACAACTTTGT CACCTTCTTC 
ATTGCGGGTC ATGAGACTTC TGCCAACCAT CTGGCATTCA CAGTGATGGA GTTGTCTCGC 
CAGCCAGAGA TTGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTTGTCGG TTCCAAGAGG 
CACCTGGACT ACGAGGATCT GGGGAGACTG CAGTATCTGT CGCAGGTCCT CAAAGAGTCT 
CTGAGGCTGT ACCCGCCAGC GTGGGGCACC TTTCGCCTGC TGGAGGAGGA GACCTTGATT 
GATGGGGTCA GAGTCCCTGG CAATACTCCT CTCCTGTTCA GCACCTACGT CATGGGGAGA 
ATGGACACCT ACTTTGAAGA CCCATTGACT TTCAACCCTG ACCGCTTCGG CCCTGGAGCG 
CCCAAGCCAC GGTTCACTTA CTTTCCCTTT TCCCTGGGCC ACCGGTCCTG CATCGGCCAA 
CAGTTTGCTC AGATGGAGGT GAAAGTTGTC ATGGCCAAGC TGCTTCAGAG GATCGAGTTC 
CGGCTAGTGC CTGGGCAGCG CTTTGGGCTG CAAGAGCAGG CTACGCTCAA GCCATTGGAC 
CCCGTGCTGT GCACCCTGAG GCCCCGGGGC TGGCAGCCTG CACCCCCACC CCCACCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu17811
Clone ID Related Accession (Same CDS sequence) NM_010010.2 , NM_010010.1
Accession Version NM_010010.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-09
Organism Mus musculus(house mouse)
Product cholesterol 24-hydroxylase precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF094479.1. On May 4, 2006 this sequence version replaced XM_981091.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF094479.1, AK140740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ATCGCGCTCG CAGCCGCTAT GAGCACATCC CCGGGCCGCC GCGGCCCAGC 
TTCCTTCTTG GACATCTCCC CTACTTTTGG AAAAAGGACG AAGATTGTGG CCGTGTGCTC 
CAAGATGTGT TTCTGGATTG GGCTAAGAAG TATGGTCCTG TTGTAAGAGT CAATGTCTTC 
TACAAGACCT CAGTCATTGT CACGAGTCCG GAGTCAGTCA AGAAGTTCCT GATGTCCACC 
AAGTACAACA AGGACTCCAA GATGTACCGC GCGCTTCAGA CTGTGTTTGG GGAGAGACTG 
TTTGGCCAGG GCTTGGTGTC TGAATGTGAC TATGGGCGCT GGTACAAGCA GAGGAAAGTC 
ATGGACTTGG CCTTCAGCCG CAGCTCCTTG GTCAGCCTGA TGGAGACGTT CAACGAGAAG 
GCGGAGCAGC TGGTGGAAAT CCTAGAAGCT AAGGCAGATG GACAGACACC CGTGTCCATG 
CAGGACATGC TGACCTGTGC CACCATCGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG 
ACCAGTATGT TACTGGGTGC CCAGAAACCG CTGTCCCAGG CAGTGAAGGT CATGCTGGAG 
GGCATCAGTG CATCCCGTAA CACCCTGGCA AAGTTCATGC CAGGGAAGAG AAAGCAGCTT 
CGGGAGATCC GCGAGAGCAT CCGTCTGCTG CGCCAGGTGG GGAAGGATTG GGTGCAGCGC 
CGCCGCGAAG CCCTGAAGAG GGGCGAGGAC ATGCCGGCTG ACATCCTCAC GCAGATCCTC 
AAAGCTGAAG AGGGAGCTCA GGACGATGAG GTTCTGCTGG ACAACTTTGT CACCTTCTTC 
ATTGCGGGTC ATGAGACTTC TGCCAACCAT CTGGCATTCA CAGTGATGGA GTTGTCTCGC 
CAGCCAGAGA TTGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTTGTCGG TTCCAAGAGG 
CACCTGGACT ACGAGGATCT GGGGAGACTG CAGTATCTGT CGCAGGTCCT CAAAGAGTCT 
CTGAGGCTGT ACCCGCCAGC GTGGGGCACC TTTCGCCTGC TGGAGGAGGA GACCTTGATT 
GATGGGGTCA GAGTCCCTGG CAATACTCCT CTCCTGTTCA GCACCTACGT CATGGGGAGA 
ATGGACACCT ACTTTGAAGA CCCATTGACT TTCAACCCTG ACCGCTTCGG CCCTGGAGCG 
CCCAAGCCAC GGTTCACTTA CTTTCCCTTT TCCCTGGGCC ACCGGTCCTG CATCGGCCAA 
CAGTTTGCTC AGATGGAGGT GAAAGTTGTC ATGGCCAAGC TGCTTCAGAG GATCGAGTTC 
CGGCTAGTGC CTGGGCAGCG CTTTGGGCTG CAAGAGCAGG CTACGCTCAA GCCATTGGAC 
CCCGTGCTGT GCACCCTGAG GCCCCGGGGC TGGCAGCCTG CACCCCCACC CCCACCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_034140.1
CDS66..1568
Translation

Target ORF information:

RefSeq Version NM_010010.1
Organism Mus musculus(house mouse)
Definition Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_010010.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAGCCCCG GGCTGCTGCT GCTCGGCAGC GCCGTCCTGC TCGCCTTCGG CCTCTGCTGC 
ACCTTCGTGC ATCGCGCTCG CAGCCGCTAT GAGCACATCC CCGGGCCGCC GCGGCCCAGC 
TTCCTTCTTG GACATCTCCC CTACTTTTGG AAAAAGGACG AAGATTGTGG CCGTGTGCTC 
CAAGATGTGT TTCTGGATTG GGCTAAGAAG TATGGTCCTG TTGTAAGAGT CAATGTCTTC 
TACAAGACCT CAGTCATTGT CACGAGTCCG GAGTCAGTCA AGAAGTTCCT GATGTCCACC 
AAGTACAACA AGGACTCCAA GATGTACCGC GCGCTTCAGA CTGTGTTTGG GGAGAGACTG 
TTTGGCCAGG GCTTGGTGTC TGAATGTGAC TATGGGCGCT GGTACAAGCA GAGGAAAGTC 
ATGGACTTGG CCTTCAGCCG CAGCTCCTTG GTCAGCCTGA TGGAGACGTT CAACGAGAAG 
GCGGAGCAGC TGGTGGAAAT CCTAGAAGCT AAGGCAGATG GACAGACACC CGTGTCCATG 
CAGGACATGC TGACCTGTGC CACCATCGAC ATCCTGGCCA AGGCAGCTTT TGGGATGGAG 
ACCAGTATGT TACTGGGTGC CCAGAAACCG CTGTCCCAGG CAGTGAAGGT CATGCTGGAG 
GGCATCAGTG CATCCCGTAA CACCCTGGCA AAGTTCATGC CAGGGAAGAG AAAGCAGCTT 
CGGGAGATCC GCGAGAGCAT CCGTCTGCTG CGCCAGGTGG GGAAGGATTG GGTGCAGCGC 
CGCCGCGAAG CCCTGAAGAG GGGCGAGGAC ATGCCGGCTG ACATCCTCAC GCAGATCCTC 
AAAGCTGAAG AGGGAGCTCA GGACGATGAG GTTCTGCTGG ACAACTTTGT CACCTTCTTC 
ATTGCGGGTC ATGAGACTTC TGCCAACCAT CTGGCATTCA CAGTGATGGA GTTGTCTCGC 
CAGCCAGAGA TTGTGGCAAG GCTGCAGGCC GAGGTGGATG AGGTTGTCGG TTCCAAGAGG 
CACCTGGACT ACGAGGATCT GGGGAGACTG CAGTATCTGT CGCAGGTCCT CAAAGAGTCT 
CTGAGGCTGT ACCCGCCAGC GTGGGGCACC TTTCGCCTGC TGGAGGAGGA GACCTTGATT 
GATGGGGTCA GAGTCCCTGG CAATACTCCT CTCCTGTTCA GCACCTACGT CATGGGGAGA 
ATGGACACCT ACTTTGAAGA CCCATTGACT TTCAACCCTG ACCGCTTCGG CCCTGGAGCG 
CCCAAGCCAC GGTTCACTTA CTTTCCCTTT TCCCTGGGCC ACCGGTCCTG CATCGGCCAA 
CAGTTTGCTC AGATGGAGGT GAAAGTTGTC ATGGCCAAGC TGCTTCAGAG GATCGAGTTC 
CGGCTAGTGC CTGGGCAGCG CTTTGGGCTG CAAGAGCAGG CTACGCTCAA GCCATTGGAC 
CCCGTGCTGT GCACCCTGAG GCCCCGGGGC TGGCAGCCTG CACCCCCACC CCCACCCTGC 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu47568
Clone ID Related Accession (Same CDS sequence) XM_030246535.1 , XM_006515456.3
Accession Version XM_006515456.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 807bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product cholesterol 24-hydroxylase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515456.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCCAGGGA AGAGAAAGCA GCTTCGGGAG ATCCGCGAGA GCATCCGTCT GCTGCGCCAG 
GTGGGGAAGG ATTGGGTGCA GCGCCGCCGC GAAGCCCTGA AGAGGGGCGA GGACATGCCG 
GCTGACATCC TCACGCAGAT CCTCAAAGCT GAAGAGGGAG CTCAGGACGA TGAGGTTCTG 
CTGGACAACT TTGTCACCTT CTTCATTGCG GGTCATGAGA CTTCTGCCAA CCATCTGGCA 
TTCACAGTGA TGGAGTTGTC TCGCCAGCCA GAGATTGTGG CAAGGCTGCA GGCCGAGGTG 
GATGAGGTTG TCGGTTCCAA GAGGCACCTG GACTACGAGG ATCTGGGGAG ACTGCAGTAT 
CTGTCGCAGG TCCTCAAAGA GTCTCTGAGG CTGTACCCGC CAGCGTGGGG CACCTTTCGC 
CTGCTGGAGG AGGAGACCTT GATTGATGGG GTCAGAGTCC CTGGCAATAC TCCTCTCCTG 
TTCAGCACCT ACGTCATGGG GAGAATGGAC ACCTACTTTG AAGACCCATT GACTTTCAAC 
CCTGACCGCT TCGGCCCTGG AGCGCCCAAG CCACGGTTCA CTTACTTTCC CTTTTCCCTG 
GGCCACCGGT CCTGCATCGG CCAACAGTTT GCTCAGATGG AGGTGAAAGT TGTCATGGCC 
AAGCTGCTTC AGAGGATCGA GTTCCGGCTA GTGCCTGGGC AGCGCTTTGG GCTGCAAGAG 
CAGGCTACGC TCAAGCCATT GGACCCCGTG CTGTGCACCC TGAGGCCCCG GGGCTGGCAG 
CCTGCACCCC CACCCCCACC CTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006515519.1
CDS1543..2349
Translation

Target ORF information:

RefSeq Version XM_006515456.3
Organism Mus musculus(house mouse)
Definition Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006515456.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCCAGGGA AGAGAAAGCA GCTTCGGGAG ATCCGCGAGA GCATCCGTCT GCTGCGCCAG 
GTGGGGAAGG ATTGGGTGCA GCGCCGCCGC GAAGCCCTGA AGAGGGGCGA GGACATGCCG 
GCTGACATCC TCACGCAGAT CCTCAAAGCT GAAGAGGGAG CTCAGGACGA TGAGGTTCTG 
CTGGACAACT TTGTCACCTT CTTCATTGCG GGTCATGAGA CTTCTGCCAA CCATCTGGCA 
TTCACAGTGA TGGAGTTGTC TCGCCAGCCA GAGATTGTGG CAAGGCTGCA GGCCGAGGTG 
GATGAGGTTG TCGGTTCCAA GAGGCACCTG GACTACGAGG ATCTGGGGAG ACTGCAGTAT 
CTGTCGCAGG TCCTCAAAGA GTCTCTGAGG CTGTACCCGC CAGCGTGGGG CACCTTTCGC 
CTGCTGGAGG AGGAGACCTT GATTGATGGG GTCAGAGTCC CTGGCAATAC TCCTCTCCTG 
TTCAGCACCT ACGTCATGGG GAGAATGGAC ACCTACTTTG AAGACCCATT GACTTTCAAC 
CCTGACCGCT TCGGCCCTGG AGCGCCCAAG CCACGGTTCA CTTACTTTCC CTTTTCCCTG 
GGCCACCGGT CCTGCATCGG CCAACAGTTT GCTCAGATGG AGGTGAAAGT TGTCATGGCC 
AAGCTGCTTC AGAGGATCGA GTTCCGGCTA GTGCCTGGGC AGCGCTTTGG GCTGCAAGAG 
CAGGCTACGC TCAAGCCATT GGACCCCGTG CTGTGCACCC TGAGGCCCCG GGGCTGGCAG 
CCTGCACCCC CACCCCCACC CTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu47568
Clone ID Related Accession (Same CDS sequence) XM_030246535.1 , XM_006515456.3
Accession Version XM_030246535.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 807bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product cholesterol 24-hydroxylase isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCCAGGGA AGAGAAAGCA GCTTCGGGAG ATCCGCGAGA GCATCCGTCT GCTGCGCCAG 
GTGGGGAAGG ATTGGGTGCA GCGCCGCCGC GAAGCCCTGA AGAGGGGCGA GGACATGCCG 
GCTGACATCC TCACGCAGAT CCTCAAAGCT GAAGAGGGAG CTCAGGACGA TGAGGTTCTG 
CTGGACAACT TTGTCACCTT CTTCATTGCG GGTCATGAGA CTTCTGCCAA CCATCTGGCA 
TTCACAGTGA TGGAGTTGTC TCGCCAGCCA GAGATTGTGG CAAGGCTGCA GGCCGAGGTG 
GATGAGGTTG TCGGTTCCAA GAGGCACCTG GACTACGAGG ATCTGGGGAG ACTGCAGTAT 
CTGTCGCAGG TCCTCAAAGA GTCTCTGAGG CTGTACCCGC CAGCGTGGGG CACCTTTCGC 
CTGCTGGAGG AGGAGACCTT GATTGATGGG GTCAGAGTCC CTGGCAATAC TCCTCTCCTG 
TTCAGCACCT ACGTCATGGG GAGAATGGAC ACCTACTTTG AAGACCCATT GACTTTCAAC 
CCTGACCGCT TCGGCCCTGG AGCGCCCAAG CCACGGTTCA CTTACTTTCC CTTTTCCCTG 
GGCCACCGGT CCTGCATCGG CCAACAGTTT GCTCAGATGG AGGTGAAAGT TGTCATGGCC 
AAGCTGCTTC AGAGGATCGA GTTCCGGCTA GTGCCTGGGC AGCGCTTTGG GCTGCAAGAG 
CAGGCTACGC TCAAGCCATT GGACCCCGTG CTGTGCACCC TGAGGCCCCG GGGCTGGCAG 
CCTGCACCCC CACCCCCACC CTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_030102395.1
CDS612..1418
Translation

Target ORF information:

RefSeq Version XM_030246535.1
Organism Mus musculus(house mouse)
Definition Mus musculus cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_030246535.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCCAGGGA AGAGAAAGCA GCTTCGGGAG ATCCGCGAGA GCATCCGTCT GCTGCGCCAG 
GTGGGGAAGG ATTGGGTGCA GCGCCGCCGC GAAGCCCTGA AGAGGGGCGA GGACATGCCG 
GCTGACATCC TCACGCAGAT CCTCAAAGCT GAAGAGGGAG CTCAGGACGA TGAGGTTCTG 
CTGGACAACT TTGTCACCTT CTTCATTGCG GGTCATGAGA CTTCTGCCAA CCATCTGGCA 
TTCACAGTGA TGGAGTTGTC TCGCCAGCCA GAGATTGTGG CAAGGCTGCA GGCCGAGGTG 
GATGAGGTTG TCGGTTCCAA GAGGCACCTG GACTACGAGG ATCTGGGGAG ACTGCAGTAT 
CTGTCGCAGG TCCTCAAAGA GTCTCTGAGG CTGTACCCGC CAGCGTGGGG CACCTTTCGC 
CTGCTGGAGG AGGAGACCTT GATTGATGGG GTCAGAGTCC CTGGCAATAC TCCTCTCCTG 
TTCAGCACCT ACGTCATGGG GAGAATGGAC ACCTACTTTG AAGACCCATT GACTTTCAAC 
CCTGACCGCT TCGGCCCTGG AGCGCCCAAG CCACGGTTCA CTTACTTTCC CTTTTCCCTG 
GGCCACCGGT CCTGCATCGG CCAACAGTTT GCTCAGATGG AGGTGAAAGT TGTCATGGCC 
AAGCTGCTTC AGAGGATCGA GTTCCGGCTA GTGCCTGGGC AGCGCTTTGG GCTGCAAGAG 
CAGGCTACGC TCAAGCCATT GGACCCCGTG CTGTGCACCC TGAGGCCCCG GGGCTGGCAG 
CCTGCACCCC CACCCCCACC CTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Cholesterol-metabolizing enzyme cytochrome P450 46A1 as a pharmacologic target for Alzheimer's disease.
Neuropharmacology123465-476(2017 Sep)
Mast N,Saadane A,Valencia-Olvera A,Constans J,Maxfield E,Arakawa H,Li Y,Landreth G,Pikuleva IA


book

Transcriptional and post-translational changes in the brain of mice deficient in cholesterol removal mediated by cytochrome P450 46A1 (CYP46A1).
PloS one12(10)e0187168(2017)
Mast N,Lin JB,Anderson KW,Bjorkhem I,Pikuleva IA


book

24S-hydroxycholesterol and 25-hydroxycholesterol differentially impact hippocampal neuronal survival following oxygen-glucose deprivation.
PloS one12(3)e0174416(2017)
Sun MY,Taylor A,Zorumski CF,Mennerick S